CSPI03G05210 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI03G05210
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	fatty acid desaturase 4, chloroplastic
Location	Chr3: 4228228 .. 4229169 (+)
RNA-Seq Expression	CSPI03G05210
Synteny	CSPI03G05210

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCCATCTTAGCTCAAAACAAGTTCCTTCTGAGCTCTCGGTCCCATGTTAATCCATGCAGCACTCCGATTCCACGCAGAGTTGTCTACTGCATAGCTAATACCACCGTCAATGGTGTTGCTACCAGTCCGAAGCATGCTGATGGTCAACTAGTCATTGAGCGTGAGGTTATATCTCCTACTCCACTTACATTTGTCAATTCCTCCACCTATTATTCTGTTCTTAATGACCCGAGTTTACGATCAACTTGGAGTCATCGTGCGTGGGTGGCTGGTGGGTGCACCACAGTGCTAATCTCTTTGGCAAAATCAATAGTGGGTGCAGCCAGTTCACAAATCTGGCTTGAGCCTGCTTTAGCAGCATTGGTAGGCTACATTTTAGCTGACCTTGGATCCGGAGTATATCATTGGGGCATCGATAACTATGGTGATGCGTCAACCCCAATCTTCGGTGCTCAAATAGAAGCATTCCAAGGCCATCACAAGTGGCCCTGGACAATCACGAAGCGACAGTTCGCCAACAATTTGTACGCTTTGGCACGAGCTGTGACTTTTGCTGTACTTCCTGTAAGCCTTGCATTTGATGATCCCATTCTACATGGTTTTGTTGGTATGTGCTCAGGTTGCATAATGTTTAGCCAACAGTTTCATGCATGGGCTCACAGTAAAAAGAGTGAACTACCTCCACTCGTGGTGGCAATGCAAAATGCTGGCCTGCTCGTGTCACGAACACAGCATTCAACACATCATCGTCCACCCTATAACAACAACTATTGCATAGTAAGTGGAATATGGAATAAGCTTTTGGATGAAACAAAGCTTTTTGAGGCATTGGAGATGATCTTGTACTTCAAACTTGGGCTGAGACCAAGATCTTGGACTGAGCCTAATTCTGAATGGACTGAAGAGACCGAGATTCTTGAACAAACTACAGCCCATTAA

mRNA sequence

ATGTCCATCTTAGCTCAAAACAAGTTCCTTCTGAGCTCTCGGTCCCATGTTAATCCATGCAGCACTCCGATTCCACGCAGAGTTGTCTACTGCATAGCTAATACCACCGTCAATGGTGTTGCTACCAGTCCGAAGCATGCTGATGGTCAACTAGTCATTGAGCGTGAGGTTATATCTCCTACTCCACTTACATTTGTCAATTCCTCCACCTATTATTCTGTTCTTAATGACCCGAGTTTACGATCAACTTGGAGTCATCGTGCGTGGGTGGCTGGTGGGTGCACCACAGTGCTAATCTCTTTGGCAAAATCAATAGTGGGTGCAGCCAGTTCACAAATCTGGCTTGAGCCTGCTTTAGCAGCATTGGTAGGCTACATTTTAGCTGACCTTGGATCCGGAGTATATCATTGGGGCATCGATAACTATGGTGATGCGTCAACCCCAATCTTCGGTGCTCAAATAGAAGCATTCCAAGGCCATCACAAGTGGCCCTGGACAATCACGAAGCGACAGTTCGCCAACAATTTGTACGCTTTGGCACGAGCTGTGACTTTTGCTGTACTTCCTGTAAGCCTTGCATTTGATGATCCCATTCTACATGGTTTTGTTGGTATGTGCTCAGGTTGCATAATGTTTAGCCAACAGTTTCATGCATGGGCTCACAGTAAAAAGAGTGAACTACCTCCACTCGTGGTGGCAATGCAAAATGCTGGCCTGCTCGTGTCACGAACACAGCATTCAACACATCATCGTCCACCCTATAACAACAACTATTGCATAGTAAGTGGAATATGGAATAAGCTTTTGGATGAAACAAAGCTTTTTGAGGCATTGGAGATGATCTTGTACTTCAAACTTGGGCTGAGACCAAGATCTTGGACTGAGCCTAATTCTGAATGGACTGAAGAGACCGAGATTCTTGAACAAACTACAGCCCATTAA

Coding sequence (CDS)

ATGTCCATCTTAGCTCAAAACAAGTTCCTTCTGAGCTCTCGGTCCCATGTTAATCCATGCAGCACTCCGATTCCACGCAGAGTTGTCTACTGCATAGCTAATACCACCGTCAATGGTGTTGCTACCAGTCCGAAGCATGCTGATGGTCAACTAGTCATTGAGCGTGAGGTTATATCTCCTACTCCACTTACATTTGTCAATTCCTCCACCTATTATTCTGTTCTTAATGACCCGAGTTTACGATCAACTTGGAGTCATCGTGCGTGGGTGGCTGGTGGGTGCACCACAGTGCTAATCTCTTTGGCAAAATCAATAGTGGGTGCAGCCAGTTCACAAATCTGGCTTGAGCCTGCTTTAGCAGCATTGGTAGGCTACATTTTAGCTGACCTTGGATCCGGAGTATATCATTGGGGCATCGATAACTATGGTGATGCGTCAACCCCAATCTTCGGTGCTCAAATAGAAGCATTCCAAGGCCATCACAAGTGGCCCTGGACAATCACGAAGCGACAGTTCGCCAACAATTTGTACGCTTTGGCACGAGCTGTGACTTTTGCTGTACTTCCTGTAAGCCTTGCATTTGATGATCCCATTCTACATGGTTTTGTTGGTATGTGCTCAGGTTGCATAATGTTTAGCCAACAGTTTCATGCATGGGCTCACAGTAAAAAGAGTGAACTACCTCCACTCGTGGTGGCAATGCAAAATGCTGGCCTGCTCGTGTCACGAACACAGCATTCAACACATCATCGTCCACCCTATAACAACAACTATTGCATAGTAAGTGGAATATGGAATAAGCTTTTGGATGAAACAAAGCTTTTTGAGGCATTGGAGATGATCTTGTACTTCAAACTTGGGCTGAGACCAAGATCTTGGACTGAGCCTAATTCTGAATGGACTGAAGAGACCGAGATTCTTGAACAAACTACAGCCCATTAA

Protein sequence

MSILAQNKFLLSSRSHVNPCSTPIPRRVVYCIANTTVNGVATSPKHADGQLVIEREVISPTPLTFVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSQIWLEPALAALVGYILADLGSGVYHWGIDNYGDASTPIFGAQIEAFQGHHKWPWTITKRQFANNLYALARAVTFAVLPVSLAFDDPILHGFVGMCSGCIMFSQQFHAWAHSKKSELPPLVVAMQNAGLLVSRTQHSTHHRPPYNNNYCIVSGIWNKLLDETKLFEALEMILYFKLGLRPRSWTEPNSEWTEETEILEQTTAH*

Homology

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BLAST of CSPI03G05210 vs. ExPASy Swiss-Prot
Match: Q9SZ42 (Fatty acid desaturase 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FAD4 PE=1 SV=1)

HSP 1 Score: 387.5 bits (994), Expect = 1.4e-106
Identity = 183/292 (62.67%), Postives = 221/292 (75.68%), Query Frame = 0


Query: 23  PIPRRVVYCIANTTVN--------GVATSPKHADGQLVIEREVISPTPLTFVNSSTYYSV 82
           P P R    +   TVN          +T P+    +LV+E+ + SP PL+          
Sbjct: 39  PQPNREKLLVEQRTVNLPLSNDQSLQSTKPRPNREKLVVEQRLASP-PLS---------- 98

Query: 83  LNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSQIWLEPALAALVGYILADLGSGV 142
            NDP+L+STW+HR WVA GCTT+ +SLAKS++G   S + LEPALA   GYILADLGSGV
Sbjct: 99  -NDPTLKSTWTHRLWVAAGCTTLFVSLAKSVIGGFDSHLCLEPALAGYAGYILADLGSGV 158

Query: 143 YHWGIDNYGDASTPIFGAQIEAFQGHHKWPWTITKRQFANNLYALARAVTFAVLPVSLAF 202
           YHW IDNYGD STP+ G QIEAFQGHHKWPWTIT+RQFANNL+ALA+ +TF VLP+ LAF
Sbjct: 159 YHWAIDNYGDESTPVVGTQIEAFQGHHKWPWTITRRQFANNLHALAQVITFTVLPLDLAF 218

Query: 203 DDPILHGFVGMCSGCIMFSQQFHAWAHSKKSELPPLVVAMQNAGLLVSRTQHSTHHRPPY 262
           +DP+ HGFV   + CI+FSQQFHAWAH  KS+LPPLVVA+Q+ GLLVSR QH+ HHR PY
Sbjct: 219 NDPVFHGFVCTFAFCILFSQQFHAWAHGTKSKLPPLVVALQDMGLLVSRRQHAEHHRAPY 278

Query: 263 NNNYCIVSGIWNKLLDETKLFEALEMILYFKLGLRPRSWTEPNSEWTEETEI 307
           NNNYCIVSG WN +LDE+K+FEALEM+ YF+LG+RPRSW+EPNS+W EETEI
Sbjct: 279 NNNYCIVSGAWNNVLDESKVFEALEMVFYFQLGVRPRSWSEPNSDWIEETEI 318

[Close]

BLAST of CSPI03G05210 vs. ExPASy Swiss-Prot
Match: O04584 (Fatty acid desaturase 4-like 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FAD4L1 PE=3 SV=1)

HSP 1 Score: 360.1 bits (923), Expect = 2.5e-98
Identity = 168/284 (59.15%), Postives = 209/284 (73.59%), Query Frame = 0


Query: 22  TPIPRRVVYCIANTTVNGVATSPKHADGQLVIEREVISPTPLTFVNSSTYYSVLNDPSLR 81
           T IPR         T +   T+P+     LV+E+ +++P PL         S  NDP+L+
Sbjct: 15  TNIPRSYGPTRVRVTCSVTTTNPQLNHENLVVEKRLVNP-PL---------SKNNDPTLQ 74

Query: 82  STWSHRAWVAGGCTTVLISLAKSIVGAASSQIWLEPALAALVGYILADLGSGVYHWGIDN 141
           STW+HR WVA G TT+  S AKSI+G   S +WL+PALA   GY+ ADLGSGVYHW IDN
Sbjct: 75  STWTHRLWVAAGSTTIFASFAKSIIGGFGSHLWLQPALACYAGYVFADLGSGVYHWAIDN 134

Query: 142 YGDASTPIFGAQIEAFQGHHKWPWTITKRQFANNLYALARAVTFAVLPVSLAFDDPILHG 201
           YG ASTPI GAQ+EA QGHHK+PWTITKRQFANN Y +ARA+TF VLP++LA ++P+ H 
Sbjct: 135 YGGASTPIVGAQLEASQGHHKYPWTITKRQFANNSYTIARAITFIVLPLNLAINNPLFHS 194

Query: 202 FVGMCSGCIMFSQQFHAWAHSKKSELPPLVVAMQNAGLLVSRTQHSTHHRPPYNNNYCIV 261
           FV   + CI+ SQQFHAWAH  KS+LPPLV+A+Q+ GLLVSR  H  HH+ PYN+NYC+V
Sbjct: 195 FVSTFAFCILLSQQFHAWAHGTKSKLPPLVMALQDMGLLVSRKDHPGHHQAPYNSNYCVV 254

Query: 262 SGIWNKLLDETKLFEALEMILYFKLGLRPRSWTEPNSEWTEETE 306
           SG WNK+LDE+ LF+ALEM L+F+ G+RP SW EPNS+WTEETE
Sbjct: 255 SGAWNKVLDESNLFKALEMALFFQFGVRPNSWNEPNSDWTEETE 288

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BLAST of CSPI03G05210 vs. ExPASy Swiss-Prot
Match: O81006 (Fatty acid desaturase 4-like 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FAD4L2 PE=2 SV=1)

HSP 1 Score: 353.2 bits (905), Expect = 3.0e-96
Identity = 167/291 (57.39%), Postives = 212/291 (72.85%), Query Frame = 0


Query: 11  LSSRSHVNPCSTPIPR--RVVYCIANTTVNGVATSPKHADGQLVIEREVISPTPLTFVNS 70
           L ++  +NP +  IPR  R  +    +T N   + P H + +LV+E+ +++P PL+    
Sbjct: 5   LQTKYTLNPITNNIPRSHRPSFLRVTSTTN---SQPNH-EMKLVVEQRLVNP-PLS---- 64

Query: 71  STYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSQIWLEPALAALVGYILA 130
                  NDP+L+STW+HR WVA GCTTV +S +KSI+GA  S +WLEP+LA   GYILA
Sbjct: 65  -------NDPTLQSTWTHRLWVAAGCTTVFVSFSKSIIGAFGSHLWLEPSLAGFAGYILA 124

Query: 131 DLGSGVYHWGIDNYGDASTPIFGAQIEAFQGHHKWPWTITKRQFANNLYALARAVTFAVL 190
           DLGSGVYHW  DNYGD STP+ G  IE  Q HHK PWTITKRQFANNL+ +AR  T  VL
Sbjct: 125 DLGSGVYHWATDNYGDESTPLVGIHIEDSQDHHKCPWTITKRQFANNLHFMARGTTLIVL 184

Query: 191 PVSLAFDDPILHGFVGMCSGCIMFSQQFHAWAHSKKSELPPLVVAMQNAGLLVSRTQHST 250
           P+ LAFDD ++HGFV M + C++F Q FHAWAH  KS+LPPLVV +Q+ GLLVSR  H  
Sbjct: 185 PLDLAFDDHVVHGFVSMFAFCVLFCQLFHAWAHGTKSKLPPLVVGLQDIGLLVSRIHHMN 244

Query: 251 HHRPPYNNNYCIVSGIWNKLLDETKLFEALEMILYFKLGLRPRSWTEPNSE 300
           HHR PYNNNYC+VSG+WNK+LDE+ +F+A+EM+LY +LG+RPRSWTEPN E
Sbjct: 245 HHRAPYNNNYCVVSGVWNKVLDESNVFKAMEMVLYIQLGVRPRSWTEPNYE 279

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BLAST of CSPI03G05210 vs. ExPASy Swiss-Prot
Match: Q9AE87 (Plasmanylethanolamine desaturase OS=Myxococcus xanthus OX=34 GN=carF PE=1 SV=1)

HSP 1 Score: 95.9 bits (237), Expect = 8.7e-19
Identity = 65/199 (32.66%), Postives = 89/199 (44.72%), Query Frame = 0


Query: 99  ISLAKSIVGAASSQIWLEPALAALVGYILADLGSGVYHWGIDNYGDASTPIFG-AQIEAF 158
           ++L   + G   +  WL  + A L+GY+ AD  SG  HW  D +G    P+ G A I  F
Sbjct: 42  VALVYRLWGTPYAGTWLLLS-AVLLGYLAADFVSGFVHWMGDTWGSTEMPVLGKALIRPF 101

Query: 159 QGHHKWPWTITKRQFA-----NNLYALARAVTFAVLPVSLAFDDPILHGFVGMCSGCIMF 218
           + HH     IT+  F      N L +L  A+    LP+S          F+G     +M 
Sbjct: 102 REHHVDEKAITRHDFVETNGNNCLISLPVAIIALCLPMS-GPGWVFCASFLGAMIFWVMA 161

Query: 219 SQQFHAWAHSKKSELPPLVVAMQNAGLLVSRTQHSTHHRPPYNNNYCIVSGIWNKLLDET 278
           + QFH W+H      P LV  +Q   L++    H  HH  PYN  YCI  G  NK L   
Sbjct: 162 TNQFHKWSHMDSP--PALVGFLQRVHLILPPDHHRIHHTKPYNKYYCITVGWMNKPLTMV 221

Query: 279 KLFEALEMILYFKLGLRPR 292
             F   E ++ +  GL PR
Sbjct: 222 HFFPTAERLITWATGLLPR 236

[Close]

BLAST of CSPI03G05210 vs. ExPASy Swiss-Prot
Match: Q5UR78 (Probable fatty acid desaturase MIMI_L630 OS=Acanthamoeba polyphaga mimivirus OX=212035 GN=MIMI_L630 PE=3 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 1.7e-11
Identity = 47/179 (26.26%), Postives = 78/179 (43.58%), Query Frame = 0


Query: 115 LEPALAALVGYILADLGSGVYHWGIDNYGDASTPIFGAQ-IEAFQGHHKWPWTITKRQFA 174
           ++  +  ++GY+L+D   G+YHW  D Y    TPI G   I   + HH  P  + +    
Sbjct: 6   IDKFIQIVLGYLLSDFIMGIYHWIKDTYFSPFTPIIGKTFIWGSRLHHVRPRYVLEFTDK 65

Query: 175 NNLYALARAVTFAVLPVSLAFDDPILHGFVGMCSGCIMFSQQFHAWAHSKKSELPPLVVA 234
           + +   A+     + P+   F    L  F+      I  +   H + H    E P     
Sbjct: 66  DLIIDSAKWTLSWIGPLFFWFG---LTPFLVTMFIMISLNDVIHKYTHEIDHERPMWATI 125

Query: 235 MQNAGLLVSRTQHSTHHRPPYNNNYCIVSGIWNKLLDETKLFEALEMILYFKLGLRPRS 293
           +Q  G   S  +H  HH  P+  NYC V+   N  L++  L+  LE  + +  G++PR+
Sbjct: 126 LQRIGFFQSHDEHHLHHIAPHEINYCPVTPYVNIWLEKINLWRKLESFVEYLTGVKPRA 181

[Close]

BLAST of CSPI03G05210 vs. ExPASy TrEMBL
Match: A0A0A0L6B9 (TMEM189_B_dmain domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G077710 PE=3 SV=1)

HSP 1 Score: 642.1 bits (1655), Expect = 1.2e-180
Identity = 310/313 (99.04%), Postives = 310/313 (99.04%), Query Frame = 0


Query: 1   MSILAQNKFLLSSRSHVNPCSTPIPRRVVYCIANTTVNGVATSPKHADGQLVIEREVISP 60
           MSILAQNKFLLSSRSHVNPCSTPIPRRVVYCIANTTVNGVATSPKHADGQLVIEREVISP
Sbjct: 1   MSILAQNKFLLSSRSHVNPCSTPIPRRVVYCIANTTVNGVATSPKHADGQLVIEREVISP 60

Query: 61  TPLTFVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSQIWLEPALA 120
           TPLTFVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASS IWLEPALA
Sbjct: 61  TPLTFVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSHIWLEPALA 120

Query: 121 ALVGYILADLGSGVYHWGIDNYGDASTPIFGAQIEAFQGHHKWPWTITKRQFANNLYALA 180
           ALVGYILADLGSGVYHWGIDNYGDASTPIFG QIEAFQGHHKWPWTITKRQFANNLYALA
Sbjct: 121 ALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKWPWTITKRQFANNLYALA 180

Query: 181 RAVTFAVLPVSLAFDDPILHGFVGMCSGCIMFSQQFHAWAHSKKSELPPLVVAMQNAGLL 240
           RAVTFAVLPVSLA DDPILHGFVGMCSGCIMFSQQFHAWAHSKKSELPPLVVAMQNAGLL
Sbjct: 181 RAVTFAVLPVSLAIDDPILHGFVGMCSGCIMFSQQFHAWAHSKKSELPPLVVAMQNAGLL 240

Query: 241 VSRTQHSTHHRPPYNNNYCIVSGIWNKLLDETKLFEALEMILYFKLGLRPRSWTEPNSEW 300
           VSRTQHSTHHRPPYNNNYCIVSGIWNKLLDETKLFEALEMILYFKLGLRPRSWTEPNSEW
Sbjct: 241 VSRTQHSTHHRPPYNNNYCIVSGIWNKLLDETKLFEALEMILYFKLGLRPRSWTEPNSEW 300

Query: 301 TEETEILEQTTAH 314
           TEETEILEQTTAH
Sbjct: 301 TEETEILEQTTAH 313

[Close]

BLAST of CSPI03G05210 vs. ExPASy TrEMBL
Match: A0A5A7SXQ7 (Fatty acid desaturase 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold925G00380 PE=3 SV=1)

HSP 1 Score: 619.4 bits (1596), Expect = 8.3e-174
Identity = 297/313 (94.89%), Postives = 305/313 (97.44%), Query Frame = 0


Query: 1   MSILAQNKFLLSSRSHVNPCSTPIPRRVVYCIANTTVNGVATSPKHADGQLVIEREVISP 60
           MSILAQNKFLLSSRSHVNPCSTP PRRVVYCIANTTVNGVATS KH DGQLVIEREVISP
Sbjct: 1   MSILAQNKFLLSSRSHVNPCSTPTPRRVVYCIANTTVNGVATSSKHGDGQLVIEREVISP 60

Query: 61  TPLTFVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSQIWLEPALA 120
           +PLT VNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASS+IWLEPALA
Sbjct: 61  SPLTSVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSRIWLEPALA 120

Query: 121 ALVGYILADLGSGVYHWGIDNYGDASTPIFGAQIEAFQGHHKWPWTITKRQFANNLYALA 180
           ALVGYILADLGSGVYHWGIDNYG ASTPIFG QIEAFQGHHKWPWTITKRQFANNLYALA
Sbjct: 121 ALVGYILADLGSGVYHWGIDNYGGASTPIFGTQIEAFQGHHKWPWTITKRQFANNLYALA 180

Query: 181 RAVTFAVLPVSLAFDDPILHGFVGMCSGCIMFSQQFHAWAHSKKSELPPLVVAMQNAGLL 240
           RAVTFAVLPVSLAFDDP LHGFVG+CSGCIMFSQQFHAWAH KKSE+PPLVVAMQ+AGLL
Sbjct: 181 RAVTFAVLPVSLAFDDPTLHGFVGVCSGCIMFSQQFHAWAHGKKSEIPPLVVAMQDAGLL 240

Query: 241 VSRTQHSTHHRPPYNNNYCIVSGIWNKLLDETKLFEALEMILYFKLGLRPRSWTEPNSEW 300
           VSRTQH+THHRPPYNNNYCIVSGIWN+LLDETK+FEALEMILYFKLGLRPRSWTEPNSEW
Sbjct: 241 VSRTQHATHHRPPYNNNYCIVSGIWNQLLDETKVFEALEMILYFKLGLRPRSWTEPNSEW 300

Query: 301 TEETEILEQTTAH 314
           TEETEILEQTTAH
Sbjct: 301 TEETEILEQTTAH 313

[Close]

BLAST of CSPI03G05210 vs. ExPASy TrEMBL
Match: A0A1S3CJN9 (fatty acid desaturase 4, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103501595 PE=3 SV=1)

HSP 1 Score: 617.5 bits (1591), Expect = 3.2e-173
Identity = 295/313 (94.25%), Postives = 304/313 (97.12%), Query Frame = 0


Query: 1   MSILAQNKFLLSSRSHVNPCSTPIPRRVVYCIANTTVNGVATSPKHADGQLVIEREVISP 60
           MSILAQNKFLLSSRSHVNPCSTP PRRVVYCIANTTVNGV TS KH DGQLVIEREVISP
Sbjct: 1   MSILAQNKFLLSSRSHVNPCSTPTPRRVVYCIANTTVNGVGTSSKHGDGQLVIEREVISP 60

Query: 61  TPLTFVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSQIWLEPALA 120
           +PLT VNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASS+IWLEPALA
Sbjct: 61  SPLTSVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSRIWLEPALA 120

Query: 121 ALVGYILADLGSGVYHWGIDNYGDASTPIFGAQIEAFQGHHKWPWTITKRQFANNLYALA 180
           ALVGYILADLGSG+YHWGIDNYG ASTPIFG QIEAFQGHHKWPWTITKRQFANNLYALA
Sbjct: 121 ALVGYILADLGSGIYHWGIDNYGGASTPIFGTQIEAFQGHHKWPWTITKRQFANNLYALA 180

Query: 181 RAVTFAVLPVSLAFDDPILHGFVGMCSGCIMFSQQFHAWAHSKKSELPPLVVAMQNAGLL 240
           RAVTFAVLPVSLAFDDP LHGFVG+CSGCIMFSQQFHAWAH KKSE+PPLVVAMQ+AGLL
Sbjct: 181 RAVTFAVLPVSLAFDDPTLHGFVGVCSGCIMFSQQFHAWAHGKKSEIPPLVVAMQDAGLL 240

Query: 241 VSRTQHSTHHRPPYNNNYCIVSGIWNKLLDETKLFEALEMILYFKLGLRPRSWTEPNSEW 300
           VSRTQH+THHRPPYNNNYCIVSGIWN+LLDETK+FEALEMILYFKLGLRPRSWTEPNSEW
Sbjct: 241 VSRTQHATHHRPPYNNNYCIVSGIWNQLLDETKVFEALEMILYFKLGLRPRSWTEPNSEW 300

Query: 301 TEETEILEQTTAH 314
           TEETEILEQTTAH
Sbjct: 301 TEETEILEQTTAH 313

[Close]

BLAST of CSPI03G05210 vs. ExPASy TrEMBL
Match: A0A6J1DD70 (fatty acid desaturase 4, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111019014 PE=3 SV=1)

HSP 1 Score: 550.8 bits (1418), Expect = 3.6e-153
Identity = 263/316 (83.23%), Postives = 283/316 (89.56%), Query Frame = 0


Query: 1   MSILAQNKFLLSSRSHVNPCSTPIPRRVVYCIANT----TVNGVATSPKHADGQLVIERE 60
           MSILAQNKFLLSSR+ VNP S PIPR++VYC+ANT          T PKHADGQLVI+RE
Sbjct: 1   MSILAQNKFLLSSRTRVNPLSPPIPRKIVYCVANTVDVAAAGATTTKPKHADGQLVIKRE 60

Query: 61  VISPTPLTFVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSQIWLE 120
            +SP PLT   +STY+ VLNDPSLRSTW HRAWVA GCTTVLISLAKSIVGAASS IWLE
Sbjct: 61  FVSP-PLTLGKASTYHYVLNDPSLRSTWGHRAWVASGCTTVLISLAKSIVGAASSHIWLE 120

Query: 121 PALAALVGYILADLGSGVYHWGIDNYGDASTPIFGAQIEAFQGHHKWPWTITKRQFANNL 180
           P LAALVGYILADLGSGVYHWGIDNYGDASTPIFG QIEAFQGHHKWPWTITKRQFANNL
Sbjct: 121 PTLAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKWPWTITKRQFANNL 180

Query: 181 YALARAVTFAVLPVSLAFDDPILHGFVGMCSGCIMFSQQFHAWAHSKKSELPPLVVAMQN 240
           YALARAVTF VLP++LAF DPILHGFV +CSGCIMFSQQFHAWAH KKS+LPPLVVAMQ+
Sbjct: 181 YALARAVTFVVLPINLAFGDPILHGFVSVCSGCIMFSQQFHAWAHDKKSQLPPLVVAMQD 240

Query: 241 AGLLVSRTQHSTHHRPPYNNNYCIVSGIWNKLLDETKLFEALEMILYFKLGLRPRSWTEP 300
           AGLLVSR QHS HHRPPYNNNYCIVSGIWN+LLDETK+FEA EMIL+FKLG+RPRSW+EP
Sbjct: 241 AGLLVSRMQHSAHHRPPYNNNYCIVSGIWNQLLDETKVFEAFEMILFFKLGVRPRSWSEP 300

Query: 301 NSEWTEETEILEQTTA 313
           N+EWTEETE +EQTTA
Sbjct: 301 NTEWTEETETVEQTTA 315

[Close]

BLAST of CSPI03G05210 vs. ExPASy TrEMBL
Match: A0A6J1IGF1 (fatty acid desaturase 4, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111476701 PE=3 SV=1)

HSP 1 Score: 539.7 bits (1389), Expect = 8.4e-150
Identity = 261/318 (82.08%), Postives = 280/318 (88.05%), Query Frame = 0


Query: 1   MSILAQNKFLLSSRSHVNPCSTPIPRRVVYCIANTTVN-----GVATSPKHADGQLVIER 60
           MSIL QNKFLLSS++HV+  S  +PR+ V CIANTTVN       A  PKH DGQLVIER
Sbjct: 1   MSILVQNKFLLSSKTHVSLWSPAVPRKFVCCIANTTVNVAAATTAAAKPKHGDGQLVIER 60

Query: 61  EVISPTPLTFVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSQIWL 120
           E +SPT     NSSTYYSVLNDPSL+STWSHR WV  GCT VLISLAKSI GAASS  WL
Sbjct: 61  EPLSPTTPVLPNSSTYYSVLNDPSLQSTWSHRVWVTSGCTIVLISLAKSIAGAASSHTWL 120

Query: 121 EPALAALVGYILADLGSGVYHWGIDNYGDASTPIFGAQIEAFQGHHKWPWTITKRQFANN 180
           EPALAALVGYILADLGSGVYHWGIDNYGDASTPIFG QIEAFQGHHK PWTITKRQFANN
Sbjct: 121 EPALAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKRPWTITKRQFANN 180

Query: 181 LYALARAVTFAVLPVSLAFDDPILHGFVGMCSGCIMFSQQFHAWAHSKKSELPPLVVAMQ 240
           LYALARAVTF VLP++LA +DPILHGFV +CSGCIMFSQQFHAWAH KKS+LPPLVVAMQ
Sbjct: 181 LYALARAVTFTVLPITLAVNDPILHGFVAVCSGCIMFSQQFHAWAHDKKSQLPPLVVAMQ 240

Query: 241 NAGLLVSRTQHSTHHRPPYNNNYCIVSGIWNKLLDETKLFEALEMILYFKLGLRPRSWTE 300
           +AGLLVSR QHS HHRPPYNNNYCIVSGIWNKLLDETK+FEALEMIL+FKLG+RPRSWTE
Sbjct: 241 DAGLLVSRLQHSAHHRPPYNNNYCIVSGIWNKLLDETKVFEALEMILFFKLGVRPRSWTE 300

Query: 301 PNSEWTEETEILEQTTAH 314
           P+SEWTEETEI+EQTTA+
Sbjct: 301 PHSEWTEETEIIEQTTAY 318

[Close]

BLAST of CSPI03G05210 vs. NCBI nr
Match: XP_004152938.1 (fatty acid desaturase 4, chloroplastic [Cucumis sativus] >KGN56137.1 hypothetical protein Csa_011564 [Cucumis sativus])

HSP 1 Score: 642.1 bits (1655), Expect = 2.5e-180
Identity = 310/313 (99.04%), Postives = 310/313 (99.04%), Query Frame = 0


Query: 1   MSILAQNKFLLSSRSHVNPCSTPIPRRVVYCIANTTVNGVATSPKHADGQLVIEREVISP 60
           MSILAQNKFLLSSRSHVNPCSTPIPRRVVYCIANTTVNGVATSPKHADGQLVIEREVISP
Sbjct: 1   MSILAQNKFLLSSRSHVNPCSTPIPRRVVYCIANTTVNGVATSPKHADGQLVIEREVISP 60

Query: 61  TPLTFVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSQIWLEPALA 120
           TPLTFVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASS IWLEPALA
Sbjct: 61  TPLTFVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSHIWLEPALA 120

Query: 121 ALVGYILADLGSGVYHWGIDNYGDASTPIFGAQIEAFQGHHKWPWTITKRQFANNLYALA 180
           ALVGYILADLGSGVYHWGIDNYGDASTPIFG QIEAFQGHHKWPWTITKRQFANNLYALA
Sbjct: 121 ALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKWPWTITKRQFANNLYALA 180

Query: 181 RAVTFAVLPVSLAFDDPILHGFVGMCSGCIMFSQQFHAWAHSKKSELPPLVVAMQNAGLL 240
           RAVTFAVLPVSLA DDPILHGFVGMCSGCIMFSQQFHAWAHSKKSELPPLVVAMQNAGLL
Sbjct: 181 RAVTFAVLPVSLAIDDPILHGFVGMCSGCIMFSQQFHAWAHSKKSELPPLVVAMQNAGLL 240

Query: 241 VSRTQHSTHHRPPYNNNYCIVSGIWNKLLDETKLFEALEMILYFKLGLRPRSWTEPNSEW 300
           VSRTQHSTHHRPPYNNNYCIVSGIWNKLLDETKLFEALEMILYFKLGLRPRSWTEPNSEW
Sbjct: 241 VSRTQHSTHHRPPYNNNYCIVSGIWNKLLDETKLFEALEMILYFKLGLRPRSWTEPNSEW 300

Query: 301 TEETEILEQTTAH 314
           TEETEILEQTTAH
Sbjct: 301 TEETEILEQTTAH 313

[Close]

BLAST of CSPI03G05210 vs. NCBI nr
Match: KAA0033959.1 (fatty acid desaturase 4 [Cucumis melo var. makuwa] >TYK02585.1 fatty acid desaturase 4 [Cucumis melo var. makuwa])

HSP 1 Score: 619.4 bits (1596), Expect = 1.7e-173
Identity = 297/313 (94.89%), Postives = 305/313 (97.44%), Query Frame = 0


Query: 1   MSILAQNKFLLSSRSHVNPCSTPIPRRVVYCIANTTVNGVATSPKHADGQLVIEREVISP 60
           MSILAQNKFLLSSRSHVNPCSTP PRRVVYCIANTTVNGVATS KH DGQLVIEREVISP
Sbjct: 1   MSILAQNKFLLSSRSHVNPCSTPTPRRVVYCIANTTVNGVATSSKHGDGQLVIEREVISP 60

Query: 61  TPLTFVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSQIWLEPALA 120
           +PLT VNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASS+IWLEPALA
Sbjct: 61  SPLTSVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSRIWLEPALA 120

Query: 121 ALVGYILADLGSGVYHWGIDNYGDASTPIFGAQIEAFQGHHKWPWTITKRQFANNLYALA 180
           ALVGYILADLGSGVYHWGIDNYG ASTPIFG QIEAFQGHHKWPWTITKRQFANNLYALA
Sbjct: 121 ALVGYILADLGSGVYHWGIDNYGGASTPIFGTQIEAFQGHHKWPWTITKRQFANNLYALA 180

Query: 181 RAVTFAVLPVSLAFDDPILHGFVGMCSGCIMFSQQFHAWAHSKKSELPPLVVAMQNAGLL 240
           RAVTFAVLPVSLAFDDP LHGFVG+CSGCIMFSQQFHAWAH KKSE+PPLVVAMQ+AGLL
Sbjct: 181 RAVTFAVLPVSLAFDDPTLHGFVGVCSGCIMFSQQFHAWAHGKKSEIPPLVVAMQDAGLL 240

Query: 241 VSRTQHSTHHRPPYNNNYCIVSGIWNKLLDETKLFEALEMILYFKLGLRPRSWTEPNSEW 300
           VSRTQH+THHRPPYNNNYCIVSGIWN+LLDETK+FEALEMILYFKLGLRPRSWTEPNSEW
Sbjct: 241 VSRTQHATHHRPPYNNNYCIVSGIWNQLLDETKVFEALEMILYFKLGLRPRSWTEPNSEW 300

Query: 301 TEETEILEQTTAH 314
           TEETEILEQTTAH
Sbjct: 301 TEETEILEQTTAH 313

[Close]

BLAST of CSPI03G05210 vs. NCBI nr
Match: XP_008463431.1 (PREDICTED: fatty acid desaturase 4, chloroplastic [Cucumis melo])

HSP 1 Score: 617.5 bits (1591), Expect = 6.5e-173
Identity = 295/313 (94.25%), Postives = 304/313 (97.12%), Query Frame = 0


Query: 1   MSILAQNKFLLSSRSHVNPCSTPIPRRVVYCIANTTVNGVATSPKHADGQLVIEREVISP 60
           MSILAQNKFLLSSRSHVNPCSTP PRRVVYCIANTTVNGV TS KH DGQLVIEREVISP
Sbjct: 1   MSILAQNKFLLSSRSHVNPCSTPTPRRVVYCIANTTVNGVGTSSKHGDGQLVIEREVISP 60

Query: 61  TPLTFVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSQIWLEPALA 120
           +PLT VNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASS+IWLEPALA
Sbjct: 61  SPLTSVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSRIWLEPALA 120

Query: 121 ALVGYILADLGSGVYHWGIDNYGDASTPIFGAQIEAFQGHHKWPWTITKRQFANNLYALA 180
           ALVGYILADLGSG+YHWGIDNYG ASTPIFG QIEAFQGHHKWPWTITKRQFANNLYALA
Sbjct: 121 ALVGYILADLGSGIYHWGIDNYGGASTPIFGTQIEAFQGHHKWPWTITKRQFANNLYALA 180

Query: 181 RAVTFAVLPVSLAFDDPILHGFVGMCSGCIMFSQQFHAWAHSKKSELPPLVVAMQNAGLL 240
           RAVTFAVLPVSLAFDDP LHGFVG+CSGCIMFSQQFHAWAH KKSE+PPLVVAMQ+AGLL
Sbjct: 181 RAVTFAVLPVSLAFDDPTLHGFVGVCSGCIMFSQQFHAWAHGKKSEIPPLVVAMQDAGLL 240

Query: 241 VSRTQHSTHHRPPYNNNYCIVSGIWNKLLDETKLFEALEMILYFKLGLRPRSWTEPNSEW 300
           VSRTQH+THHRPPYNNNYCIVSGIWN+LLDETK+FEALEMILYFKLGLRPRSWTEPNSEW
Sbjct: 241 VSRTQHATHHRPPYNNNYCIVSGIWNQLLDETKVFEALEMILYFKLGLRPRSWTEPNSEW 300

Query: 301 TEETEILEQTTAH 314
           TEETEILEQTTAH
Sbjct: 301 TEETEILEQTTAH 313

[Close]

BLAST of CSPI03G05210 vs. NCBI nr
Match: XP_038905900.1 (fatty acid desaturase 4, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 601.7 bits (1550), Expect = 3.7e-168
Identity = 288/317 (90.85%), Postives = 301/317 (94.95%), Query Frame = 0


Query: 1   MSILAQNKFLLSSRSHVNPCSTPIPRRVVYCIANTTVNGVA----TSPKHADGQLVIERE 60
           MSILAQNKFLLSSRSHVNPCSTPIPRRV YCIAN+TVN  A    T+PKHADGQL+IERE
Sbjct: 1   MSILAQNKFLLSSRSHVNPCSTPIPRRVFYCIANSTVNVAAGATTTNPKHADGQLLIERE 60

Query: 61  VISPTPLTFVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSQIWLE 120
           VISPTPLT VNSSTYYSVLNDPSLRSTWSHRAWVA GCTTVL+SLAKSIVGAASS IWLE
Sbjct: 61  VISPTPLTLVNSSTYYSVLNDPSLRSTWSHRAWVASGCTTVLVSLAKSIVGAASSHIWLE 120

Query: 121 PALAALVGYILADLGSGVYHWGIDNYGDASTPIFGAQIEAFQGHHKWPWTITKRQFANNL 180
           PALAALVGYILADLGSGVYHWGIDNYG+ STPIFG QIEAFQGHHKWPWTITKRQFANN+
Sbjct: 121 PALAALVGYILADLGSGVYHWGIDNYGNVSTPIFGTQIEAFQGHHKWPWTITKRQFANNM 180

Query: 181 YALARAVTFAVLPVSLAFDDPILHGFVGMCSGCIMFSQQFHAWAHSKKSELPPLVVAMQN 240
           YALARAVTFAVLPVSLAFDDPILHGFVG+CSGCIMFSQQFHAWAH KKSELPPLVVAMQ+
Sbjct: 181 YALARAVTFAVLPVSLAFDDPILHGFVGVCSGCIMFSQQFHAWAHGKKSELPPLVVAMQD 240

Query: 241 AGLLVSRTQHSTHHRPPYNNNYCIVSGIWNKLLDETKLFEALEMILYFKLGLRPRSWTEP 300
           AGLLVSR QHS HHRPPYNNNYCIVSGIWNK+LDETK+FEALEMIL+FKLG+RPRSWTEP
Sbjct: 241 AGLLVSRRQHSAHHRPPYNNNYCIVSGIWNKILDETKVFEALEMILFFKLGVRPRSWTEP 300

Query: 301 NSEWTEETEILEQTTAH 314
           NS WTEETEI+EQTTAH
Sbjct: 301 NSAWTEETEIIEQTTAH 317

[Close]

BLAST of CSPI03G05210 vs. NCBI nr
Match: XP_022150986.1 (fatty acid desaturase 4, chloroplastic [Momordica charantia])

HSP 1 Score: 550.8 bits (1418), Expect = 7.5e-153
Identity = 263/316 (83.23%), Postives = 283/316 (89.56%), Query Frame = 0


Query: 1   MSILAQNKFLLSSRSHVNPCSTPIPRRVVYCIANT----TVNGVATSPKHADGQLVIERE 60
           MSILAQNKFLLSSR+ VNP S PIPR++VYC+ANT          T PKHADGQLVI+RE
Sbjct: 1   MSILAQNKFLLSSRTRVNPLSPPIPRKIVYCVANTVDVAAAGATTTKPKHADGQLVIKRE 60

Query: 61  VISPTPLTFVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSQIWLE 120
            +SP PLT   +STY+ VLNDPSLRSTW HRAWVA GCTTVLISLAKSIVGAASS IWLE
Sbjct: 61  FVSP-PLTLGKASTYHYVLNDPSLRSTWGHRAWVASGCTTVLISLAKSIVGAASSHIWLE 120

Query: 121 PALAALVGYILADLGSGVYHWGIDNYGDASTPIFGAQIEAFQGHHKWPWTITKRQFANNL 180
           P LAALVGYILADLGSGVYHWGIDNYGDASTPIFG QIEAFQGHHKWPWTITKRQFANNL
Sbjct: 121 PTLAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKWPWTITKRQFANNL 180

Query: 181 YALARAVTFAVLPVSLAFDDPILHGFVGMCSGCIMFSQQFHAWAHSKKSELPPLVVAMQN 240
           YALARAVTF VLP++LAF DPILHGFV +CSGCIMFSQQFHAWAH KKS+LPPLVVAMQ+
Sbjct: 181 YALARAVTFVVLPINLAFGDPILHGFVSVCSGCIMFSQQFHAWAHDKKSQLPPLVVAMQD 240

Query: 241 AGLLVSRTQHSTHHRPPYNNNYCIVSGIWNKLLDETKLFEALEMILYFKLGLRPRSWTEP 300
           AGLLVSR QHS HHRPPYNNNYCIVSGIWN+LLDETK+FEA EMIL+FKLG+RPRSW+EP
Sbjct: 241 AGLLVSRMQHSAHHRPPYNNNYCIVSGIWNQLLDETKVFEAFEMILFFKLGVRPRSWSEP 300

Query: 301 NSEWTEETEILEQTTA 313
           N+EWTEETE +EQTTA
Sbjct: 301 NTEWTEETETVEQTTA 315

[Close]

BLAST of CSPI03G05210 vs. TAIR 10
Match: AT4G27030.1 (fatty acid desaturase A )

HSP 1 Score: 387.5 bits (994), Expect = 1.0e-107
Identity = 183/292 (62.67%), Postives = 221/292 (75.68%), Query Frame = 0


Query: 23  PIPRRVVYCIANTTVN--------GVATSPKHADGQLVIEREVISPTPLTFVNSSTYYSV 82
           P P R    +   TVN          +T P+    +LV+E+ + SP PL+          
Sbjct: 39  PQPNREKLLVEQRTVNLPLSNDQSLQSTKPRPNREKLVVEQRLASP-PLS---------- 98

Query: 83  LNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSQIWLEPALAALVGYILADLGSGV 142
            NDP+L+STW+HR WVA GCTT+ +SLAKS++G   S + LEPALA   GYILADLGSGV
Sbjct: 99  -NDPTLKSTWTHRLWVAAGCTTLFVSLAKSVIGGFDSHLCLEPALAGYAGYILADLGSGV 158

Query: 143 YHWGIDNYGDASTPIFGAQIEAFQGHHKWPWTITKRQFANNLYALARAVTFAVLPVSLAF 202
           YHW IDNYGD STP+ G QIEAFQGHHKWPWTIT+RQFANNL+ALA+ +TF VLP+ LAF
Sbjct: 159 YHWAIDNYGDESTPVVGTQIEAFQGHHKWPWTITRRQFANNLHALAQVITFTVLPLDLAF 218

Query: 203 DDPILHGFVGMCSGCIMFSQQFHAWAHSKKSELPPLVVAMQNAGLLVSRTQHSTHHRPPY 262
           +DP+ HGFV   + CI+FSQQFHAWAH  KS+LPPLVVA+Q+ GLLVSR QH+ HHR PY
Sbjct: 219 NDPVFHGFVCTFAFCILFSQQFHAWAHGTKSKLPPLVVALQDMGLLVSRRQHAEHHRAPY 278

Query: 263 NNNYCIVSGIWNKLLDETKLFEALEMILYFKLGLRPRSWTEPNSEWTEETEI 307
           NNNYCIVSG WN +LDE+K+FEALEM+ YF+LG+RPRSW+EPNS+W EETEI
Sbjct: 279 NNNYCIVSGAWNNVLDESKVFEALEMVFYFQLGVRPRSWSEPNSDWIEETEI 318

[Close]

BLAST of CSPI03G05210 vs. TAIR 10
Match: AT1G62190.1 (Kua-ubiquitin conjugating enzyme hybrid localisation domain )

HSP 1 Score: 360.1 bits (923), Expect = 1.7e-99
Identity = 168/284 (59.15%), Postives = 209/284 (73.59%), Query Frame = 0


Query: 22  TPIPRRVVYCIANTTVNGVATSPKHADGQLVIEREVISPTPLTFVNSSTYYSVLNDPSLR 81
           T IPR         T +   T+P+     LV+E+ +++P PL         S  NDP+L+
Sbjct: 15  TNIPRSYGPTRVRVTCSVTTTNPQLNHENLVVEKRLVNP-PL---------SKNNDPTLQ 74

Query: 82  STWSHRAWVAGGCTTVLISLAKSIVGAASSQIWLEPALAALVGYILADLGSGVYHWGIDN 141
           STW+HR WVA G TT+  S AKSI+G   S +WL+PALA   GY+ ADLGSGVYHW IDN
Sbjct: 75  STWTHRLWVAAGSTTIFASFAKSIIGGFGSHLWLQPALACYAGYVFADLGSGVYHWAIDN 134

Query: 142 YGDASTPIFGAQIEAFQGHHKWPWTITKRQFANNLYALARAVTFAVLPVSLAFDDPILHG 201
           YG ASTPI GAQ+EA QGHHK+PWTITKRQFANN Y +ARA+TF VLP++LA ++P+ H 
Sbjct: 135 YGGASTPIVGAQLEASQGHHKYPWTITKRQFANNSYTIARAITFIVLPLNLAINNPLFHS 194

Query: 202 FVGMCSGCIMFSQQFHAWAHSKKSELPPLVVAMQNAGLLVSRTQHSTHHRPPYNNNYCIV 261
           FV   + CI+ SQQFHAWAH  KS+LPPLV+A+Q+ GLLVSR  H  HH+ PYN+NYC+V
Sbjct: 195 FVSTFAFCILLSQQFHAWAHGTKSKLPPLVMALQDMGLLVSRKDHPGHHQAPYNSNYCVV 254

Query: 262 SGIWNKLLDETKLFEALEMILYFKLGLRPRSWTEPNSEWTEETE 306
           SG WNK+LDE+ LF+ALEM L+F+ G+RP SW EPNS+WTEETE
Sbjct: 255 SGAWNKVLDESNLFKALEMALFFQFGVRPNSWNEPNSDWTEETE 288

[Close]

BLAST of CSPI03G05210 vs. TAIR 10
Match: AT2G22890.1 (Kua-ubiquitin conjugating enzyme hybrid localisation domain )

HSP 1 Score: 353.2 bits (905), Expect = 2.1e-97
Identity = 167/291 (57.39%), Postives = 212/291 (72.85%), Query Frame = 0


Query: 11  LSSRSHVNPCSTPIPR--RVVYCIANTTVNGVATSPKHADGQLVIEREVISPTPLTFVNS 70
           L ++  +NP +  IPR  R  +    +T N   + P H + +LV+E+ +++P PL+    
Sbjct: 5   LQTKYTLNPITNNIPRSHRPSFLRVTSTTN---SQPNH-EMKLVVEQRLVNP-PLS---- 64

Query: 71  STYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSQIWLEPALAALVGYILA 130
                  NDP+L+STW+HR WVA GCTTV +S +KSI+GA  S +WLEP+LA   GYILA
Sbjct: 65  -------NDPTLQSTWTHRLWVAAGCTTVFVSFSKSIIGAFGSHLWLEPSLAGFAGYILA 124

Query: 131 DLGSGVYHWGIDNYGDASTPIFGAQIEAFQGHHKWPWTITKRQFANNLYALARAVTFAVL 190
           DLGSGVYHW  DNYGD STP+ G  IE  Q HHK PWTITKRQFANNL+ +AR  T  VL
Sbjct: 125 DLGSGVYHWATDNYGDESTPLVGIHIEDSQDHHKCPWTITKRQFANNLHFMARGTTLIVL 184

Query: 191 PVSLAFDDPILHGFVGMCSGCIMFSQQFHAWAHSKKSELPPLVVAMQNAGLLVSRTQHST 250
           P+ LAFDD ++HGFV M + C++F Q FHAWAH  KS+LPPLVV +Q+ GLLVSR  H  
Sbjct: 185 PLDLAFDDHVVHGFVSMFAFCVLFCQLFHAWAHGTKSKLPPLVVGLQDIGLLVSRIHHMN 244

Query: 251 HHRPPYNNNYCIVSGIWNKLLDETKLFEALEMILYFKLGLRPRSWTEPNSE 300
           HHR PYNNNYC+VSG+WNK+LDE+ +F+A+EM+LY +LG+RPRSWTEPN E
Sbjct: 245 HHRAPYNNNYCVVSGVWNKVLDESNVFKAMEMVLYIQLGVRPRSWTEPNYE 279

The following BLAST results are available for this feature:

BLAST of CSPI03G05210 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SZ42	1.4e-106	62.67	Fatty acid desaturase 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FAD4 P...	[more]
O04584	2.5e-98	59.15	Fatty acid desaturase 4-like 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN...	[more]
O81006	3.0e-96	57.39	Fatty acid desaturase 4-like 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN...	[more]
Q9AE87	8.7e-19	32.66	Plasmanylethanolamine desaturase OS=Myxococcus xanthus OX=34 GN=carF PE=1 SV=1	[more]
Q5UR78	1.7e-11	26.26	Probable fatty acid desaturase MIMI_L630 OS=Acanthamoeba polyphaga mimivirus OX=...	[more]

BLAST of CSPI03G05210 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L6B9	1.2e-180	99.04	TMEM189_B_dmain domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G07...	[more]
A0A5A7SXQ7	8.3e-174	94.89	Fatty acid desaturase 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold...	[more]
A0A1S3CJN9	3.2e-173	94.25	fatty acid desaturase 4, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103501595 P...	[more]
A0A6J1DD70	3.6e-153	83.23	fatty acid desaturase 4, chloroplastic OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A6J1IGF1	8.4e-150	82.08	fatty acid desaturase 4, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC1114767...	[more]

BLAST of CSPI03G05210 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004152938.1	2.5e-180	99.04	fatty acid desaturase 4, chloroplastic [Cucumis sativus] >KGN56137.1 hypothetica...	[more]
KAA0033959.1	1.7e-173	94.89	fatty acid desaturase 4 [Cucumis melo var. makuwa] >TYK02585.1 fatty acid desatu...	[more]
XP_008463431.1	6.5e-173	94.25	PREDICTED: fatty acid desaturase 4, chloroplastic [Cucumis melo]	[more]
XP_038905900.1	3.7e-168	90.85	fatty acid desaturase 4, chloroplastic-like [Benincasa hispida]	[more]
XP_022150986.1	7.5e-153	83.23	fatty acid desaturase 4, chloroplastic [Momordica charantia]	[more]

BLAST of CSPI03G05210 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G27030.1	1.0e-107	62.67	fatty acid desaturase A	[more]
AT1G62190.1	1.7e-99	59.15	Kua-ubiquitin conjugating enzyme hybrid localisation domain	[more]
AT2G22890.1	2.1e-97	57.39	Kua-ubiquitin conjugating enzyme hybrid localisation domain	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR019547	B domain of TMEM189, localisation domain	PFAM	PF10520	TMEM189_B_dmain	coord: 125..293 e-value: 1.5E-64 score: 217.3
None	No IPR available	PANTHER	PTHR48140	FAMILY NOT NAMED	coord: 1..309

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G05210.1	CSPI03G05210.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006631	fatty acid metabolic process
cellular_component	GO:0016021	integral component of membrane