CSPI01G23470.2 (mRNA) Cucumber (PI 183967) v1

Overview


Name	CSPI01G23470.2
Type	mRNA
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Copper transporter
Location	Chr1: 19033540 .. 19034625 (-)
Sequence length	927
RNA-Seq Expression	CSPI01G23470.2
Synteny	CSPI01G23470.2

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAATTAAAAAAAAGAAAGAGTAGGTGTCAAATGGTGAGAGGTGAACACACATGAAAAATGATAAACCGGCGGTGCACCAAATCAGGCGTTTAGTCATAGAAGTCACAATGAGTGTACGAACAAATCCATTTAATACTGAAACAGACGGCGTTCTTATCACTTATCACAAACCAATCGCCCTTCCCAATACCACACACGAGAATTTCCCGGCACCGGAGCCACCATGGACGCTGCCACCGAAGCTCACAACCATCATATGCACGGCATGACACCGCCACCGGACTCCTCCACCGCATCGCCGGAAATGATGCACCACAAGATGATGATGCACATGACCTTCTTTTGGGGAACCAACGCCGAGATTCTGTTCCATCGCTGGCCTGGCGAGCGCTCCGGCATGTACGCCTTAGCCTTAATCTTCATCTTCGTGCTTGCTTTTCTCGTCGAATGGCTTACACATTGCCGATTGATTAAGGAAGATTCGAGCCGGGCAGCGGCAGGATTGATCCGGACTCTGATGCATACGGTTAGGGTTGGATTAGCGTATTTAGTGATGCTTGCGGTGATGTCGTTCAATGTTGGAGTGCTTTTGGTAGCAATCGGTGGACATTGCTTGGGATTTTTCTTGTTTGGGAGTAAATTTTTCAAGAGATCGGAAGCGGTTTCTGGTAAATCCTCTGATCTTCCTCCTCTTAGTTGTTGATGAAGGGATAATAATGTGGAAATCTGGAATTGAAATTGAATGTTATGATAGTGTATTGAATGTTGGTGATGAATTTTTCTTGGATTGGATTAGAAACGAAATTTGTTGTTGGAATTCTTTGCAGCTTATGTCAAGTTGTAAACGGAAGAGGGGAAATGGTAAATTGGAAATGCTTTGGATTCAGATTTCAGGGATAATTCATCTTGTAATTTTTCTATCTCACTGTTCATCGTTTTCATTTAGGATTTTAATTTTGTAATTGTAAGCAAGAACTATGAAGATATTTAGTGGTGTTTAGGATTCATAGTCGCTATCTAAACTTAGAATTCATGAGTGTTCATTCATATTGTGAAATTTTTCAATGATAATGTTAAATTTTTCTA

mRNA sequence

AAATTAAAAAAAAGAAAGAGTAGGTGTCAAATGGTGAGAGGTGAACACACATGAAAAATGATAAACCGGCGGTGCACCAAATCAGGCGTTTAGTCATAGAAGTCACAATGAGTGTACGAACAAATCCATTTAATACTGAAACAGACGGCGTTCTTATCACTTATCACAAACCAATCGCCCTTCCCAATACCACACACGAGAATTTCCCGGCACCGGAGCCACCATGGACGCTGCCACCGAAGCTCACAACCATCATATGCACGGCATGACACCGCCACCGGACTCCTCCACCGCATCGCCGGAAATGATGCACCACAAGATGATGATGCACATGACCTTCTTTTGGGGAACCAACGCCGAGATTCTGTTCCATCGCTGGCCTGGCGAGCGCTCCGGCATGTACGCCTTAGCCTTAATCTTCATCTTCGTGCTTGCTTTTCTCGTCGAATGGCTTACACATTGCCGATTGATTAAGGAAGATTCGAGCCGGGCAGCGGCAGGATTGATCCGGACTCTGATGCATACGGTTAGGGTTGGATTAGCGTATTTAGTGATGCTTGCGGTGATGTCGTTCAATGTTGGAGTGCTTTTGGTAGCAATCGGTGGACATTGCTTGGGATTTTTCTTGTTTGGGAGTAAATTTTTCAAGAGATCGGAAGCGGTTTCTGCTTATGTCAAGTTGTAAACGGAAGAGGGGAAATGGTAAATTGGAAATGCTTTGGATTCAGATTTCAGGGATAATTCATCTTGTAATTTTTCTATCTCACTGTTCATCGTTTTCATTTAGGATTTTAATTTTGTAATTGTAAGCAAGAACTATGAAGATATTTAGTGGTGTTTAGGATTCATAGTCGCTATCTAAACTTAGAATTCATGAGTGTTCATTCATATTGTGAAATTTTTCAATGATAATGTTAAATTTTTCTA

Coding sequence (CDS)

ATGGACGCTGCCACCGAAGCTCACAACCATCATATGCACGGCATGACACCGCCACCGGACTCCTCCACCGCATCGCCGGAAATGATGCACCACAAGATGATGATGCACATGACCTTCTTTTGGGGAACCAACGCCGAGATTCTGTTCCATCGCTGGCCTGGCGAGCGCTCCGGCATGTACGCCTTAGCCTTAATCTTCATCTTCGTGCTTGCTTTTCTCGTCGAATGGCTTACACATTGCCGATTGATTAAGGAAGATTCGAGCCGGGCAGCGGCAGGATTGATCCGGACTCTGATGCATACGGTTAGGGTTGGATTAGCGTATTTAGTGATGCTTGCGGTGATGTCGTTCAATGTTGGAGTGCTTTTGGTAGCAATCGGTGGACATTGCTTGGGATTTTTCTTGTTTGGGAGTAAATTTTTCAAGAGATCGGAAGCGGTTTCTGCTTATGTCAAGTTGTAA

Protein sequence

MDAATEAHNHHMHGMTPPPDSSTASPEMMHHKMMMHMTFFWGTNAEILFHRWPGERSGMYALALIFIFVLAFLVEWLTHCRLIKEDSSRAAAGLIRTLMHTVRVGLAYLVMLAVMSFNVGVLLVAIGGHCLGFFLFGSKFFKRSEAVSAYVKL*

Homology

[Close]

BLAST of CSPI01G23470.2 vs. ExPASy Swiss-Prot
Match: Q39065 (Copper transporter 1 OS=Arabidopsis thaliana OX=3702 GN=COPT1 PE=2 SV=2)

HSP 1 Score: 162.5 bits (410), Expect = 3.7e-39
Identity = 86/150 (57.33%), Postives = 103/150 (68.67%), Query Frame = 0


Query: 11  HMHGMTPPPDSSTASPEMM-------------HHKMMMHMTFFWGTNAEILFHRWPGERS 70
           HMHGM  P  SS++SP  M             H KMMMHMTFFWG N E+LF  WPG  S
Sbjct: 5   HMHGMPRPSSSSSSSPSSMMNNGSMNEGGGHHHMKMMMHMTFFWGKNTEVLFSGWPGTSS 64

Query: 71  GMYALALIFIFVLAFLVEWLTHCRLIK---EDSSRAAAGLIRTLMHTVRVGLAYLVMLAV 130
           GMYAL LIF+F LA L EWL H  L++    DS+  AAGLI+T ++T+R+GLAYLVMLAV
Sbjct: 65  GMYALCLIFVFFLAVLTEWLAHSSLLRGSTGDSANRAAGLIQTAVYTLRIGLAYLVMLAV 124

Query: 131 MSFNVGVLLVAIGGHCLGFFLFGSKFFKRS 145
           MSFN GV LVA+ GH +GF LFGS+ F+ +
Sbjct: 125 MSFNAGVFLVALAGHAVGFMLFGSQTFRNT 154

[Close]

BLAST of CSPI01G23470.2 vs. ExPASy Swiss-Prot
Match: Q9STG2 (Copper transporter 2 OS=Arabidopsis thaliana OX=3702 GN=COPT2 PE=2 SV=1)

HSP 1 Score: 151.8 bits (382), Expect = 6.5e-36
Identity = 79/135 (58.52%), Postives = 97/135 (71.85%), Query Frame = 0


Query: 11  HMHGMTPP-PDSSTASPEMMHHKMMMHMTFFWGTNAEILFHRWPGERSGMYALALIFIFV 70
           HMH M PP P SS+ S     H MMMHMTFFWG N E+LF  WPG  SGMYAL LI IF+
Sbjct: 5   HMHDMPPPSPSSSSMSNHTTPHMMMMHMTFFWGKNTEVLFSGWPGTSSGMYALCLIVIFL 64

Query: 71  LAFLVEWLTHCRLIK-EDSSRAAAGLIRTLMHTVRVGLAYLVMLAVMSFNVGVLLVAIGG 130
           LA + EWL H  +++   S+  AAGL +T ++T++ GL+YLVMLAVMSFN GV +VAI G
Sbjct: 65  LAVIAEWLAHSPILRVSGSTNRAAGLAQTAVYTLKTGLSYLVMLAVMSFNAGVFIVAIAG 124

Query: 131 HCLGFFLFGSKFFKR 144
           + +GFFLFGS  FK+
Sbjct: 125 YGVGFFLFGSTTFKK 139

[Close]

BLAST of CSPI01G23470.2 vs. ExPASy Swiss-Prot
Match: Q8GWP3 (Copper transporter 6 OS=Arabidopsis thaliana OX=3702 GN=COPT6 PE=2 SV=1)

HSP 1 Score: 134.0 bits (336), Expect = 1.4e-30
Identity = 70/131 (53.44%), Postives = 88/131 (67.18%), Query Frame = 0


Query: 13  HGMTPPPDSSTASPEMMHHKMMMHMTFFWGTNAEILFHRWPGERSGMYALALIFIFVLAF 72
           HG  PP   S+       + +MMHMTFFWG N EILF  WPG   GMY L LI +F+LA 
Sbjct: 3   HGNMPPSSPSSMVNHTNSNMIMMHMTFFWGKNTEILFSGWPGTSLGMYVLCLIVVFLLAV 62

Query: 73  LVEWLTHCRLIK-EDSSRAAAGLIRTLMHTVRVGLAYLVMLAVMSFNVGVLLVAIGGHCL 132
           +VEWL H  +++   S+  A GL++T ++T++ GLAYLVMLAVMSFN GV +VAI G  +
Sbjct: 63  IVEWLAHSSILRGRGSTSRAKGLVQTAVYTLKTGLAYLVMLAVMSFNGGVFIVAIAGFAV 122

Query: 133 GFFLFGSKFFK 143
           GF LFGS  FK
Sbjct: 123 GFMLFGSTAFK 133

[Close]

BLAST of CSPI01G23470.2 vs. ExPASy Swiss-Prot
Match: Q94EE4 (Copper transporter 1 OS=Oryza sativa subsp. japonica OX=39947 GN=COPT1 PE=2 SV=1)

HSP 1 Score: 124.8 bits (312), Expect = 8.5e-28
Identity = 72/134 (53.73%), Postives = 85/134 (63.43%), Query Frame = 0


Query: 10  HHMHGMTPPPDSSTASPEMMHHKMM--MHMTFFWGTNAEILFHRWPGERSGMYALALIFI 69
           H M GM+PP   + A   M   K M   HMTFFWG N+E+LF  WPG R GMYALALIF+
Sbjct: 6   HDMGGMSPPAAGAAAQGGMGAMKSMRYTHMTFFWGKNSEVLFTMWPGTRGGMYALALIFV 65

Query: 70  FVLAFLVEWL-----THCRLIKEDSSRAAAGLIRTLMHTVRVGLAYLVMLAVMSFNVGVL 129
           F LA +VE+L       C       + AA GL R  +HTVRVG+AYL+MLA+MSFN GV 
Sbjct: 66  FALAVIVEFLGSRRADACLAALARRAPAAGGLARAAVHTVRVGVAYLLMLALMSFNGGVF 125

Query: 130 LVAIGGHCLGFFLF 137
           LVA+ GH  GF  F
Sbjct: 126 LVAVAGHAAGFLAF 139

[Close]

BLAST of CSPI01G23470.2 vs. ExPASy Swiss-Prot
Match: Q8SAA5 (Copper transporter 4 OS=Arabidopsis thaliana OX=3702 GN=COPT4 PE=2 SV=2)

HSP 1 Score: 120.2 bits (300), Expect = 2.1e-26
Identity = 61/128 (47.66%), Postives = 82/128 (64.06%), Query Frame = 0


Query: 21  SSTASPEMMHHKMMMHMTFFWGTNAEILFHRWPGERSGMYALALIFIFVLAFLVEWLTHC 80
           ++T   +  H   ++H TF+WG N ++LF  WPG   GMYALALIF+F LAFL EWL  C
Sbjct: 16  TTTTQTQTPHRPSLLHPTFYWGYNCQVLFSGWPGSDRGMYALALIFVFFLAFLAEWLARC 75

Query: 81  ---RLIKEDSSRAAAGLIRTLMHTVRVGLAYLVMLAVMSFNVGVLLVAIGGHCLGFFLFG 140
                IK+ + + A    RT M+TV+ G +YLV+LAV+SFN GV L AI GH LGF +F 
Sbjct: 76  SDASSIKQGADKLAKVAFRTAMYTVKSGFSYLVILAVVSFNGGVFLAAIFGHALGFAVFR 135

Query: 141 SKFFKRSE 146
            + F+  +
Sbjct: 136 GRAFRNRD 143

[Close]

BLAST of CSPI01G23470.2 vs. ExPASy TrEMBL
Match: A0A0A0LVF3 (Copper transporter OS=Cucumis sativus OX=3659 GN=Csa_1G526830 PE=3 SV=1)

HSP 1 Score: 307.8 bits (787), Expect = 2.6e-80
Identity = 153/153 (100.00%), Postives = 153/153 (100.00%), Query Frame = 0


Query: 1   MDAATEAHNHHMHGMTPPPDSSTASPEMMHHKMMMHMTFFWGTNAEILFHRWPGERSGMY 60
           MDAATEAHNHHMHGMTPPPDSSTASPEMMHHKMMMHMTFFWGTNAEILFHRWPGERSGMY
Sbjct: 1   MDAATEAHNHHMHGMTPPPDSSTASPEMMHHKMMMHMTFFWGTNAEILFHRWPGERSGMY 60

Query: 61  ALALIFIFVLAFLVEWLTHCRLIKEDSSRAAAGLIRTLMHTVRVGLAYLVMLAVMSFNVG 120
           ALALIFIFVLAFLVEWLTHCRLIKEDSSRAAAGLIRTLMHTVRVGLAYLVMLAVMSFNVG
Sbjct: 61  ALALIFIFVLAFLVEWLTHCRLIKEDSSRAAAGLIRTLMHTVRVGLAYLVMLAVMSFNVG 120

Query: 121 VLLVAIGGHCLGFFLFGSKFFKRSEAVSAYVKL 154
           VLLVAIGGHCLGFFLFGSKFFKRSEAVSAYVKL
Sbjct: 121 VLLVAIGGHCLGFFLFGSKFFKRSEAVSAYVKL 153

[Close]

BLAST of CSPI01G23470.2 vs. ExPASy TrEMBL
Match: A0A1S3C5M2 (Copper transporter OS=Cucumis melo OX=3656 GN=LOC103497118 PE=3 SV=1)

HSP 1 Score: 300.8 bits (769), Expect = 3.2e-78
Identity = 149/153 (97.39%), Postives = 151/153 (98.69%), Query Frame = 0


Query: 1   MDAATEAHNHHMHGMTPPPDSSTASPEMMHHKMMMHMTFFWGTNAEILFHRWPGERSGMY 60
           MDAA EAHNHHMHGMTPPPDSSTASPEMMHHKMMMHMTFFWGTNAEILFHRWPGERSGMY
Sbjct: 1   MDAAAEAHNHHMHGMTPPPDSSTASPEMMHHKMMMHMTFFWGTNAEILFHRWPGERSGMY 60

Query: 61  ALALIFIFVLAFLVEWLTHCRLIKEDSSRAAAGLIRTLMHTVRVGLAYLVMLAVMSFNVG 120
           ALALI IFVLAFLVEWLTHCRLIKEDSS+AAAGLIRTLMHTVRVGLAYLVMLAVMSFNVG
Sbjct: 61  ALALILIFVLAFLVEWLTHCRLIKEDSSQAAAGLIRTLMHTVRVGLAYLVMLAVMSFNVG 120

Query: 121 VLLVAIGGHCLGFFLFGSKFFKRSEAVSAYVKL 154
           VLLVAIGGHCLGFFLFGS+FFKRSEAVSAYVKL
Sbjct: 121 VLLVAIGGHCLGFFLFGSRFFKRSEAVSAYVKL 153

[Close]

BLAST of CSPI01G23470.2 vs. ExPASy TrEMBL
Match: A0A5A7SLZ1 (Copper transporter OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold194G001730 PE=3 SV=1)

HSP 1 Score: 291.6 bits (745), Expect = 1.9e-75
Identity = 144/148 (97.30%), Postives = 146/148 (98.65%), Query Frame = 0


Query: 1   MDAATEAHNHHMHGMTPPPDSSTASPEMMHHKMMMHMTFFWGTNAEILFHRWPGERSGMY 60
           MDAA EAHNHHMHGMTPPPDSSTASPEMMHHKMMMHMTFFWGTNAEILFHRWPGERSGMY
Sbjct: 1   MDAAAEAHNHHMHGMTPPPDSSTASPEMMHHKMMMHMTFFWGTNAEILFHRWPGERSGMY 60

Query: 61  ALALIFIFVLAFLVEWLTHCRLIKEDSSRAAAGLIRTLMHTVRVGLAYLVMLAVMSFNVG 120
           ALALI IFVLAFLVEWLTHCRLIKEDSS+AAAGLIRTLMHTVRVGLAYLVMLAVMSFNVG
Sbjct: 61  ALALILIFVLAFLVEWLTHCRLIKEDSSQAAAGLIRTLMHTVRVGLAYLVMLAVMSFNVG 120

Query: 121 VLLVAIGGHCLGFFLFGSKFFKRSEAVS 149
           VLLVAIGGHCLGFFLFGS+FFKRSEAVS
Sbjct: 121 VLLVAIGGHCLGFFLFGSRFFKRSEAVS 148

[Close]

BLAST of CSPI01G23470.2 vs. ExPASy TrEMBL
Match: A0A6J1KFM2 (Copper transporter OS=Cucurbita maxima OX=3661 GN=LOC111493432 PE=3 SV=1)

HSP 1 Score: 250.8 bits (639), Expect = 3.8e-63
Identity = 126/153 (82.35%), Postives = 132/153 (86.27%), Query Frame = 0


Query: 1   MDAATEAHNHHMHGMTPPPDSSTASPEMMHHKMMMHMTFFWGTNAEILFHRWPGERSGMY 60
           M+A  E H+HHMHGM PPP SS ASP M  H  MMHMTFFWGTNAEILF  WPG+RSGMY
Sbjct: 1   MNATDETHHHHMHGMAPPPASSAASPGMTMHHRMMHMTFFWGTNAEILFRNWPGDRSGMY 60

Query: 61  ALALIFIFVLAFLVEWLTHCRLIKEDSSRAAAGLIRTLMHTVRVGLAYLVMLAVMSFNVG 120
           ALALI IFVLAF+VEWL+H RLIKEDSS AAAGLIRTLMHTVRVGLAYLVMLAVMSFNVG
Sbjct: 61  ALALILIFVLAFIVEWLSHSRLIKEDSSAAAAGLIRTLMHTVRVGLAYLVMLAVMSFNVG 120

Query: 121 VLLVAIGGHCLGFFLFGSKFFKRSEAVSAYVKL 154
           V LVAIGGHCLGFF FGS+FFK S A SAYVKL
Sbjct: 121 VFLVAIGGHCLGFFFFGSRFFKNSGAASAYVKL 153

[Close]

BLAST of CSPI01G23470.2 vs. ExPASy TrEMBL
Match: A0A6J1G9U6 (Copper transporter OS=Cucurbita moschata OX=3662 GN=LOC111452256 PE=3 SV=1)

HSP 1 Score: 245.7 bits (626), Expect = 1.2e-61
Identity = 124/153 (81.05%), Postives = 130/153 (84.97%), Query Frame = 0


Query: 1   MDAATEAHNHHMHGMTPPPDSSTASPEMMHHKMMMHMTFFWGTNAEILFHRWPGERSGMY 60
           MDA  E H+HHM GM PPP SS AS  M  H  MMHMTFFWGTNAEILF  WPG+RSGMY
Sbjct: 1   MDATDETHHHHMQGMAPPPASSAASSGMTMHHRMMHMTFFWGTNAEILFRNWPGDRSGMY 60

Query: 61  ALALIFIFVLAFLVEWLTHCRLIKEDSSRAAAGLIRTLMHTVRVGLAYLVMLAVMSFNVG 120
           ALALIFIFVLAFLVEWL+H RLI+EDSS  AAGLIRTLMHTVRVGLAYLVMLAVMSFNVG
Sbjct: 61  ALALIFIFVLAFLVEWLSHSRLIREDSSSVAAGLIRTLMHTVRVGLAYLVMLAVMSFNVG 120

Query: 121 VLLVAIGGHCLGFFLFGSKFFKRSEAVSAYVKL 154
           V LVAIGGHCLGFF FGS+FFK S A SAY+KL
Sbjct: 121 VFLVAIGGHCLGFFFFGSRFFKNSGAASAYLKL 153

[Close]

BLAST of CSPI01G23470.2 vs. NCBI nr
Match: XP_004145275.1 (copper transporter 1 [Cucumis sativus] >XP_031741185.1 copper transporter 1-like [Cucumis sativus])

HSP 1 Score: 307.8 bits (787), Expect = 5.4e-80
Identity = 153/153 (100.00%), Postives = 153/153 (100.00%), Query Frame = 0


Query: 1   MDAATEAHNHHMHGMTPPPDSSTASPEMMHHKMMMHMTFFWGTNAEILFHRWPGERSGMY 60
           MDAATEAHNHHMHGMTPPPDSSTASPEMMHHKMMMHMTFFWGTNAEILFHRWPGERSGMY
Sbjct: 1   MDAATEAHNHHMHGMTPPPDSSTASPEMMHHKMMMHMTFFWGTNAEILFHRWPGERSGMY 60

Query: 61  ALALIFIFVLAFLVEWLTHCRLIKEDSSRAAAGLIRTLMHTVRVGLAYLVMLAVMSFNVG 120
           ALALIFIFVLAFLVEWLTHCRLIKEDSSRAAAGLIRTLMHTVRVGLAYLVMLAVMSFNVG
Sbjct: 61  ALALIFIFVLAFLVEWLTHCRLIKEDSSRAAAGLIRTLMHTVRVGLAYLVMLAVMSFNVG 120

Query: 121 VLLVAIGGHCLGFFLFGSKFFKRSEAVSAYVKL 154
           VLLVAIGGHCLGFFLFGSKFFKRSEAVSAYVKL
Sbjct: 121 VLLVAIGGHCLGFFLFGSKFFKRSEAVSAYVKL 153

[Close]

BLAST of CSPI01G23470.2 vs. NCBI nr
Match: XP_008457427.1 (PREDICTED: copper transporter 1 [Cucumis melo])

HSP 1 Score: 300.8 bits (769), Expect = 6.6e-78
Identity = 149/153 (97.39%), Postives = 151/153 (98.69%), Query Frame = 0


Query: 1   MDAATEAHNHHMHGMTPPPDSSTASPEMMHHKMMMHMTFFWGTNAEILFHRWPGERSGMY 60
           MDAA EAHNHHMHGMTPPPDSSTASPEMMHHKMMMHMTFFWGTNAEILFHRWPGERSGMY
Sbjct: 1   MDAAAEAHNHHMHGMTPPPDSSTASPEMMHHKMMMHMTFFWGTNAEILFHRWPGERSGMY 60

Query: 61  ALALIFIFVLAFLVEWLTHCRLIKEDSSRAAAGLIRTLMHTVRVGLAYLVMLAVMSFNVG 120
           ALALI IFVLAFLVEWLTHCRLIKEDSS+AAAGLIRTLMHTVRVGLAYLVMLAVMSFNVG
Sbjct: 61  ALALILIFVLAFLVEWLTHCRLIKEDSSQAAAGLIRTLMHTVRVGLAYLVMLAVMSFNVG 120

Query: 121 VLLVAIGGHCLGFFLFGSKFFKRSEAVSAYVKL 154
           VLLVAIGGHCLGFFLFGS+FFKRSEAVSAYVKL
Sbjct: 121 VLLVAIGGHCLGFFLFGSRFFKRSEAVSAYVKL 153

[Close]

BLAST of CSPI01G23470.2 vs. NCBI nr
Match: KAE8653274.1 (hypothetical protein Csa_023259 [Cucumis sativus])

HSP 1 Score: 300.1 bits (767), Expect = 1.1e-77
Identity = 149/149 (100.00%), Postives = 149/149 (100.00%), Query Frame = 0


Query: 1   MDAATEAHNHHMHGMTPPPDSSTASPEMMHHKMMMHMTFFWGTNAEILFHRWPGERSGMY 60
           MDAATEAHNHHMHGMTPPPDSSTASPEMMHHKMMMHMTFFWGTNAEILFHRWPGERSGMY
Sbjct: 1   MDAATEAHNHHMHGMTPPPDSSTASPEMMHHKMMMHMTFFWGTNAEILFHRWPGERSGMY 60

Query: 61  ALALIFIFVLAFLVEWLTHCRLIKEDSSRAAAGLIRTLMHTVRVGLAYLVMLAVMSFNVG 120
           ALALIFIFVLAFLVEWLTHCRLIKEDSSRAAAGLIRTLMHTVRVGLAYLVMLAVMSFNVG
Sbjct: 61  ALALIFIFVLAFLVEWLTHCRLIKEDSSRAAAGLIRTLMHTVRVGLAYLVMLAVMSFNVG 120

Query: 121 VLLVAIGGHCLGFFLFGSKFFKRSEAVSA 150
           VLLVAIGGHCLGFFLFGSKFFKRSEAVSA
Sbjct: 121 VLLVAIGGHCLGFFLFGSKFFKRSEAVSA 149

[Close]

BLAST of CSPI01G23470.2 vs. NCBI nr
Match: KGN66186.2 (hypothetical protein Csa_019561 [Cucumis sativus])

HSP 1 Score: 296.6 bits (758), Expect = 1.2e-76
Identity = 147/152 (96.71%), Postives = 149/152 (98.03%), Query Frame = 0


Query: 1   MDAATEAHNHHMHGMTPPPDSSTASPEMMHHKMMMHMTFFWGTNAEILFHRWPGERSGMY 60
           MDAATEAHNHHMHGMTPPPDSSTASPEMMHHKMMMHMTFFWGTNAEILFHRWPGERSGMY
Sbjct: 1   MDAATEAHNHHMHGMTPPPDSSTASPEMMHHKMMMHMTFFWGTNAEILFHRWPGERSGMY 60

Query: 61  ALALIFIFVLAFLVEWLTHCRLIKEDSSRAAAGLIRTLMHTVRVGLAYLVMLAVMSFNVG 120
           ALALIFIFVLAFLVEWLTHCRLIKEDSSRAAAGLIRTLMHTVRVGLAYLVMLAVMSFNVG
Sbjct: 61  ALALIFIFVLAFLVEWLTHCRLIKEDSSRAAAGLIRTLMHTVRVGLAYLVMLAVMSFNVG 120

Query: 121 VLLVAIGGHCLGFFLFGSKFFKRSEAVSAYVK 153
           VLLVAIGGHCLGFFLFGSKFFKRSEA+ +  K
Sbjct: 121 VLLVAIGGHCLGFFLFGSKFFKRSEALMSSCK 152

[Close]

BLAST of CSPI01G23470.2 vs. NCBI nr
Match: KAA0031770.1 (copper transporter 1 [Cucumis melo var. makuwa] >TYJ97362.1 copper transporter 1 [Cucumis melo var. makuwa])

HSP 1 Score: 291.6 bits (745), Expect = 4.0e-75
Identity = 144/148 (97.30%), Postives = 146/148 (98.65%), Query Frame = 0


Query: 1   MDAATEAHNHHMHGMTPPPDSSTASPEMMHHKMMMHMTFFWGTNAEILFHRWPGERSGMY 60
           MDAA EAHNHHMHGMTPPPDSSTASPEMMHHKMMMHMTFFWGTNAEILFHRWPGERSGMY
Sbjct: 1   MDAAAEAHNHHMHGMTPPPDSSTASPEMMHHKMMMHMTFFWGTNAEILFHRWPGERSGMY 60

Query: 61  ALALIFIFVLAFLVEWLTHCRLIKEDSSRAAAGLIRTLMHTVRVGLAYLVMLAVMSFNVG 120
           ALALI IFVLAFLVEWLTHCRLIKEDSS+AAAGLIRTLMHTVRVGLAYLVMLAVMSFNVG
Sbjct: 61  ALALILIFVLAFLVEWLTHCRLIKEDSSQAAAGLIRTLMHTVRVGLAYLVMLAVMSFNVG 120

Query: 121 VLLVAIGGHCLGFFLFGSKFFKRSEAVS 149
           VLLVAIGGHCLGFFLFGS+FFKRSEAVS
Sbjct: 121 VLLVAIGGHCLGFFLFGSRFFKRSEAVS 148

[Close]

BLAST of CSPI01G23470.2 vs. TAIR 10
Match: AT5G59030.1 (copper transporter 1 )

HSP 1 Score: 162.5 bits (410), Expect = 2.6e-40
Identity = 86/150 (57.33%), Postives = 103/150 (68.67%), Query Frame = 0


Query: 11  HMHGMTPPPDSSTASPEMM-------------HHKMMMHMTFFWGTNAEILFHRWPGERS 70
           HMHGM  P  SS++SP  M             H KMMMHMTFFWG N E+LF  WPG  S
Sbjct: 5   HMHGMPRPSSSSSSSPSSMMNNGSMNEGGGHHHMKMMMHMTFFWGKNTEVLFSGWPGTSS 64

Query: 71  GMYALALIFIFVLAFLVEWLTHCRLIK---EDSSRAAAGLIRTLMHTVRVGLAYLVMLAV 130
           GMYAL LIF+F LA L EWL H  L++    DS+  AAGLI+T ++T+R+GLAYLVMLAV
Sbjct: 65  GMYALCLIFVFFLAVLTEWLAHSSLLRGSTGDSANRAAGLIQTAVYTLRIGLAYLVMLAV 124

Query: 131 MSFNVGVLLVAIGGHCLGFFLFGSKFFKRS 145
           MSFN GV LVA+ GH +GF LFGS+ F+ +
Sbjct: 125 MSFNAGVFLVALAGHAVGFMLFGSQTFRNT 154

[Close]

BLAST of CSPI01G23470.2 vs. TAIR 10
Match: AT3G46900.1 (copper transporter 2 )

HSP 1 Score: 151.8 bits (382), Expect = 4.6e-37
Identity = 79/135 (58.52%), Postives = 97/135 (71.85%), Query Frame = 0


Query: 11  HMHGMTPP-PDSSTASPEMMHHKMMMHMTFFWGTNAEILFHRWPGERSGMYALALIFIFV 70
           HMH M PP P SS+ S     H MMMHMTFFWG N E+LF  WPG  SGMYAL LI IF+
Sbjct: 5   HMHDMPPPSPSSSSMSNHTTPHMMMMHMTFFWGKNTEVLFSGWPGTSSGMYALCLIVIFL 64

Query: 71  LAFLVEWLTHCRLIK-EDSSRAAAGLIRTLMHTVRVGLAYLVMLAVMSFNVGVLLVAIGG 130
           LA + EWL H  +++   S+  AAGL +T ++T++ GL+YLVMLAVMSFN GV +VAI G
Sbjct: 65  LAVIAEWLAHSPILRVSGSTNRAAGLAQTAVYTLKTGLSYLVMLAVMSFNAGVFIVAIAG 124

Query: 131 HCLGFFLFGSKFFKR 144
           + +GFFLFGS  FK+
Sbjct: 125 YGVGFFLFGSTTFKK 139

[Close]

BLAST of CSPI01G23470.2 vs. TAIR 10
Match: AT2G26975.1 (Ctr copper transporter family )

HSP 1 Score: 134.0 bits (336), Expect = 1.0e-31
Identity = 70/131 (53.44%), Postives = 88/131 (67.18%), Query Frame = 0


Query: 13  HGMTPPPDSSTASPEMMHHKMMMHMTFFWGTNAEILFHRWPGERSGMYALALIFIFVLAF 72
           HG  PP   S+       + +MMHMTFFWG N EILF  WPG   GMY L LI +F+LA 
Sbjct: 3   HGNMPPSSPSSMVNHTNSNMIMMHMTFFWGKNTEILFSGWPGTSLGMYVLCLIVVFLLAV 62

Query: 73  LVEWLTHCRLIK-EDSSRAAAGLIRTLMHTVRVGLAYLVMLAVMSFNVGVLLVAIGGHCL 132
           +VEWL H  +++   S+  A GL++T ++T++ GLAYLVMLAVMSFN GV +VAI G  +
Sbjct: 63  IVEWLAHSSILRGRGSTSRAKGLVQTAVYTLKTGLAYLVMLAVMSFNGGVFIVAIAGFAV 122

Query: 133 GFFLFGSKFFK 143
           GF LFGS  FK
Sbjct: 123 GFMLFGSTAFK 133

[Close]

BLAST of CSPI01G23470.2 vs. TAIR 10
Match: AT2G37925.1 (copper transporter 4 )

HSP 1 Score: 120.2 bits (300), Expect = 1.5e-27
Identity = 61/128 (47.66%), Postives = 82/128 (64.06%), Query Frame = 0


Query: 21  SSTASPEMMHHKMMMHMTFFWGTNAEILFHRWPGERSGMYALALIFIFVLAFLVEWLTHC 80
           ++T   +  H   ++H TF+WG N ++LF  WPG   GMYALALIF+F LAFL EWL  C
Sbjct: 16  TTTTQTQTPHRPSLLHPTFYWGYNCQVLFSGWPGSDRGMYALALIFVFFLAFLAEWLARC 75

Query: 81  ---RLIKEDSSRAAAGLIRTLMHTVRVGLAYLVMLAVMSFNVGVLLVAIGGHCLGFFLFG 140
                IK+ + + A    RT M+TV+ G +YLV+LAV+SFN GV L AI GH LGF +F 
Sbjct: 76  SDASSIKQGADKLAKVAFRTAMYTVKSGFSYLVILAVVSFNGGVFLAAIFGHALGFAVFR 135

Query: 141 SKFFKRSE 146
            + F+  +
Sbjct: 136 GRAFRNRD 143

[Close]

BLAST of CSPI01G23470.2 vs. TAIR 10
Match: AT5G59040.1 (copper transporter 3 )

HSP 1 Score: 111.7 bits (278), Expect = 5.3e-25
Identity = 62/143 (43.36%), Postives = 84/143 (58.74%), Query Frame = 0


Query: 12  MHGMT------PPPDSSTASPEMMHHKMMMHMTFFWGTNAEILFHRWPGERSGMYALALI 71
           M+GM+      P P  S+       H  MMHMTFFWG   E+LF  WPG    MY + L 
Sbjct: 1   MNGMSGSSPAAPAPSPSSFFQHRHRHGGMMHMTFFWGKTTEVLFDGWPGTSLKMYWVCLA 60

Query: 72  FIFVLAFLVEWLTHCRLIKEDSSRAAAGLIRTLMHTVRVGLAYLVMLAVMSFNVGVLLVA 131
            IFV++   E L+ C  +K   +    GL++T ++TVR  L+YLVMLAVMSFN GV + A
Sbjct: 61  VIFVISAFSECLSRCGFMKSGPASLGGGLLQTAVYTVRAALSYLVMLAVMSFNGGVFVAA 120

Query: 132 IGGHCLGFFLFGSKFFKRSEAVS 149
           + G  LGF +FGS+ F+ + + S
Sbjct: 121 MAGFGLGFMIFGSRAFRATSSNS 143

The following BLAST results are available for this feature:

BLAST of CSPI01G23470.2 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q39065	3.7e-39	57.33	Copper transporter 1 OS=Arabidopsis thaliana OX=3702 GN=COPT1 PE=2 SV=2	[more]
Q9STG2	6.5e-36	58.52	Copper transporter 2 OS=Arabidopsis thaliana OX=3702 GN=COPT2 PE=2 SV=1	[more]
Q8GWP3	1.4e-30	53.44	Copper transporter 6 OS=Arabidopsis thaliana OX=3702 GN=COPT6 PE=2 SV=1	[more]
Q94EE4	8.5e-28	53.73	Copper transporter 1 OS=Oryza sativa subsp. japonica OX=39947 GN=COPT1 PE=2 SV=1	[more]
Q8SAA5	2.1e-26	47.66	Copper transporter 4 OS=Arabidopsis thaliana OX=3702 GN=COPT4 PE=2 SV=2	[more]

BLAST of CSPI01G23470.2 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LVF3	2.6e-80	100.00	Copper transporter OS=Cucumis sativus OX=3659 GN=Csa_1G526830 PE=3 SV=1	[more]
A0A1S3C5M2	3.2e-78	97.39	Copper transporter OS=Cucumis melo OX=3656 GN=LOC103497118 PE=3 SV=1	[more]
A0A5A7SLZ1	1.9e-75	97.30	Copper transporter OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold194G0...	[more]
A0A6J1KFM2	3.8e-63	82.35	Copper transporter OS=Cucurbita maxima OX=3661 GN=LOC111493432 PE=3 SV=1	[more]
A0A6J1G9U6	1.2e-61	81.05	Copper transporter OS=Cucurbita moschata OX=3662 GN=LOC111452256 PE=3 SV=1	[more]

BLAST of CSPI01G23470.2 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004145275.1	5.4e-80	100.00	copper transporter 1 [Cucumis sativus] >XP_031741185.1 copper transporter 1-like...	[more]
XP_008457427.1	6.6e-78	97.39	PREDICTED: copper transporter 1 [Cucumis melo]	[more]
KAE8653274.1	1.1e-77	100.00	hypothetical protein Csa_023259 [Cucumis sativus]	[more]
KGN66186.2	1.2e-76	96.71	hypothetical protein Csa_019561 [Cucumis sativus]	[more]
KAA0031770.1	4.0e-75	97.30	copper transporter 1 [Cucumis melo var. makuwa] >TYJ97362.1 copper transporter 1...	[more]

BLAST of CSPI01G23470.2 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G59030.1	2.6e-40	57.33	copper transporter 1	[more]
AT3G46900.1	4.6e-37	58.52	copper transporter 2	[more]
AT2G26975.1	1.0e-31	53.44	Ctr copper transporter family	[more]
AT2G37925.1	1.5e-27	47.66	copper transporter 4	[more]
AT5G59040.1	5.3e-25	43.36	copper transporter 3	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR007274	Ctr copper transporter	PFAM	PF04145	Ctr	coord: 87..136 e-value: 1.0E-10 score: 42.3 coord: 37..81 e-value: 6.4E-9 score: 36.5
IPR007274	Ctr copper transporter	PANTHER	PTHR12483	SOLUTE CARRIER FAMILY 31 COPPER TRANSPORTERS	coord: 7..146
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..25
None	No IPR available	PANTHER	PTHR12483:SF80	COPPER TRANSPORTER 6-LIKE	coord: 7..146

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CSPI01G23470	CSPI01G23470	gene

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI01G23470.2.utr3p1	CSPI01G23470.2.utr3p1	three_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI01G23470.2.cds2	CSPI01G23470.2.cds2	CDS
CSPI01G23470.2.cds1	CSPI01G23470.2.cds1	CDS

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI01G23470.2.utr5p1	CSPI01G23470.2.utr5p1	five_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CSPI01G23470.2	CSPI01G23470.2-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0035434	copper ion transmembrane transport
biological_process	GO:0030242	autophagy of peroxisome
biological_process	GO:0006878	cellular copper ion homeostasis
biological_process	GO:0045324	late endosome to vacuole transport
biological_process	GO:0016236	macroautophagy
biological_process	GO:0006468	protein phosphorylation
biological_process	GO:0006623	protein targeting to vacuole
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005770	late endosome
cellular_component	GO:0071561	nucleus-vacuole junction
cellular_component	GO:0034271	phosphatidylinositol 3-kinase complex, class III, type I
cellular_component	GO:0034272	phosphatidylinositol 3-kinase complex, class III, type II
cellular_component	GO:0005886	plasma membrane
molecular_function	GO:0005375	copper ion transmembrane transporter activity
molecular_function	GO:0004674	protein serine/threonine kinase activity