CSPI01G15070.1 (mRNA) Cucumber (PI 183967) v1

Overview


Name	CSPI01G15070.1
Type	mRNA
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Poly(A)-specific ribonuclease
Location	Chr1: 10682257 .. 10683732 (-)
Sequence length	1476
RNA-Seq Expression	CSPI01G15070.1
Synteny	CSPI01G15070.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CATCCACTTACTCCGAAATACACGTCACTATTCATTTATAAATATTTTCAATTAAAGAAAAAACGTTTTAGGCAGAACCCAAATTAGACCGAGTCGACTCGCTTACTGTGTGAGTCAAGGACTAATGAAAAAAACGCGTTGAATCAAATACTCTGAACTTTACTACTCTCTCGATCCTCCCACTTTCCTTTTATATTCCTTTTCCTTTTCCATTCTCTATATTAATCTCTCCTTTCTTCTTCTTTTCCATCCAAATCCCTCACTACTTCAATCCCATCTTATTTTAGTTTTTATTTTATTTATTTTATTTAACTTTTACACCATGCTTATCCACGATCTCAACATCAACCACAAATCCATCCAAATTCGTGAAGTTTGGGCTTCCAATCTCGAATCCGAATTTCGTTTGATCCGCGATCTCATCGACCAGTTCCCTTGCGTTTCAATGGACACGGAGTTCCCCGGAGTCATTTTCCCCCAACAAGCCAAACCCAAATCTCCAAATCACCCCTCCCATCACTACCAATTCCTCAAATCCAACGTCGACCTCCTCCATCTCATCCAGATCGGAATCACTCTCTCCGATTCTGACGGAAACTTGCCAGATCTCGGCACCGGCGACACCCGATTCATCTGGGAGTTCAACTTTAAGGACTTTGACGTGGCGCGTGACCTCCACGCTCCGAACTCCATCGAGCTTCTACGCCGTCAGGGCATCGACTTCGACCGGAATCGAGAAGAAGGGATCGATTCCTCCCGCTTCGCCGAGTTGATGATGTCATCGGGACTCGTCTGTAACGACTCGGTCAGTTGGGTGACGTTTCACAGTGCCTATGATTTTGGGTATTTGGTTAAGATTTTAACCCACCAAAAACTTCCAAATGATTTAGAACAGTTCCTCGGCGTTGTTAGGCTCTTCTTCGGCGATAACGTGTACGACATGAAGTACTTGATGCGCTTCTGCCAGAGTCTATACGGCGGTTTGGACCGGATTGCGAAAACTATGAATGTGAACCGGGCGGTTGGAAAGTGCCATCAAGCCGGCTCAGATAGCTTGCTAACGTGGCATGCCTTTCAAAAGATGAGAGATACCTTTTTTGTACAGACTGAAATGCACAAACATGCTGGGGTTTTGTACGGATTAGAGGTCTTCTAATCTAAATTGTACCTTAAATAAATTTAATTTAAATACTATATACCCATTTATTATTAATTACTAATTTCCACTTCTTTTATTTAATTTCTTGAAAGGTAAAGTCTATGGAATGAAGCTATAGATGCAGGTCATTTTGGAGTCAATATTATTCAAAATATAACTACATTACTATAATGTATAATAAGTAAATATTATAGCCTCCGTTTGATAACCCTTATGGCTTCAAGACAATGGTGCATCCAAACACTCATCTTCCTAAATCTCTATCTAAGTTACCTATTATGAAATATCTTCCAACATTTTCATCCCTTACAATTAAC

mRNA sequence

CATCCACTTACTCCGAAATACACGTCACTATTCATTTATAAATATTTTCAATTAAAGAAAAAACGTTTTAGGCAGAACCCAAATTAGACCGAGTCGACTCGCTTACTGTGTGAGTCAAGGACTAATGAAAAAAACGCGTTGAATCAAATACTCTGAACTTTACTACTCTCTCGATCCTCCCACTTTCCTTTTATATTCCTTTTCCTTTTCCATTCTCTATATTAATCTCTCCTTTCTTCTTCTTTTCCATCCAAATCCCTCACTACTTCAATCCCATCTTATTTTAGTTTTTATTTTATTTATTTTATTTAACTTTTACACCATGCTTATCCACGATCTCAACATCAACCACAAATCCATCCAAATTCGTGAAGTTTGGGCTTCCAATCTCGAATCCGAATTTCGTTTGATCCGCGATCTCATCGACCAGTTCCCTTGCGTTTCAATGGACACGGAGTTCCCCGGAGTCATTTTCCCCCAACAAGCCAAACCCAAATCTCCAAATCACCCCTCCCATCACTACCAATTCCTCAAATCCAACGTCGACCTCCTCCATCTCATCCAGATCGGAATCACTCTCTCCGATTCTGACGGAAACTTGCCAGATCTCGGCACCGGCGACACCCGATTCATCTGGGAGTTCAACTTTAAGGACTTTGACGTGGCGCGTGACCTCCACGCTCCGAACTCCATCGAGCTTCTACGCCGTCAGGGCATCGACTTCGACCGGAATCGAGAAGAAGGGATCGATTCCTCCCGCTTCGCCGAGTTGATGATGTCATCGGGACTCGTCTGTAACGACTCGGTCAGTTGGGTGACGTTTCACAGTGCCTATGATTTTGGGTATTTGGTTAAGATTTTAACCCACCAAAAACTTCCAAATGATTTAGAACAGTTCCTCGGCGTTGTTAGGCTCTTCTTCGGCGATAACGTGTACGACATGAAGTACTTGATGCGCTTCTGCCAGAGTCTATACGGCGGTTTGGACCGGATTGCGAAAACTATGAATGTGAACCGGGCGGTTGGAAAGTGCCATCAAGCCGGCTCAGATAGCTTGCTAACGTGGCATGCCTTTCAAAAGATGAGAGATACCTTTTTTGTACAGACTGAAATGCACAAACATGCTGGGGTTTTGTACGGATTAGAGGTCTTCTAATCTAAATTGTACCTTAAATAAATTTAATTTAAATACTATATACCCATTTATTATTAATTACTAATTTCCACTTCTTTTATTTAATTTCTTGAAAGGTAAAGTCTATGGAATGAAGCTATAGATGCAGGTCATTTTGGAGTCAATATTATTCAAAATATAACTACATTACTATAATGTATAATAAGTAAATATTATAGCCTCCGTTTGATAACCCTTATGGCTTCAAGACAATGGTGCATCCAAACACTCATCTTCCTAAATCTCTATCTAAGTTACCTATTATGAAATATCTTCCAACATTTTCATCCCTTACAATTAAC

Coding sequence (CDS)

ATGCTTATCCACGATCTCAACATCAACCACAAATCCATCCAAATTCGTGAAGTTTGGGCTTCCAATCTCGAATCCGAATTTCGTTTGATCCGCGATCTCATCGACCAGTTCCCTTGCGTTTCAATGGACACGGAGTTCCCCGGAGTCATTTTCCCCCAACAAGCCAAACCCAAATCTCCAAATCACCCCTCCCATCACTACCAATTCCTCAAATCCAACGTCGACCTCCTCCATCTCATCCAGATCGGAATCACTCTCTCCGATTCTGACGGAAACTTGCCAGATCTCGGCACCGGCGACACCCGATTCATCTGGGAGTTCAACTTTAAGGACTTTGACGTGGCGCGTGACCTCCACGCTCCGAACTCCATCGAGCTTCTACGCCGTCAGGGCATCGACTTCGACCGGAATCGAGAAGAAGGGATCGATTCCTCCCGCTTCGCCGAGTTGATGATGTCATCGGGACTCGTCTGTAACGACTCGGTCAGTTGGGTGACGTTTCACAGTGCCTATGATTTTGGGTATTTGGTTAAGATTTTAACCCACCAAAAACTTCCAAATGATTTAGAACAGTTCCTCGGCGTTGTTAGGCTCTTCTTCGGCGATAACGTGTACGACATGAAGTACTTGATGCGCTTCTGCCAGAGTCTATACGGCGGTTTGGACCGGATTGCGAAAACTATGAATGTGAACCGGGCGGTTGGAAAGTGCCATCAAGCCGGCTCAGATAGCTTGCTAACGTGGCATGCCTTTCAAAAGATGAGAGATACCTTTTTTGTACAGACTGAAATGCACAAACATGCTGGGGTTTTGTACGGATTAGAGGTCTTCTAA

Protein sequence

MLIHDLNINHKSIQIREVWASNLESEFRLIRDLIDQFPCVSMDTEFPGVIFPQQAKPKSPNHPSHHYQFLKSNVDLLHLIQIGITLSDSDGNLPDLGTGDTRFIWEFNFKDFDVARDLHAPNSIELLRRQGIDFDRNREEGIDSSRFAELMMSSGLVCNDSVSWVTFHSAYDFGYLVKILTHQKLPNDLEQFLGVVRLFFGDNVYDMKYLMRFCQSLYGGLDRIAKTMNVNRAVGKCHQAGSDSLLTWHAFQKMRDTFFVQTEMHKHAGVLYGLEVF*

Homology

[Close]

BLAST of CSPI01G15070.1 vs. ExPASy Swiss-Prot
Match: Q9FMS6 (Probable CCR4-associated factor 1 homolog 11 OS=Arabidopsis thaliana OX=3702 GN=CAF1-11 PE=2 SV=1)

HSP 1 Score: 390.2 bits (1001), Expect = 2.0e-107
Identity = 185/267 (69.29%), Postives = 222/267 (83.15%), Query Frame = 0


Query: 13  IQIREVWASNLESEFRLIRDLIDQFPCVSMDTEFPGVIFPQQAKPKSPNHPSHHYQFLKS 72
           I IR+VWA NLESEF LIR +++ +P +SMDTEFPGVI+          +P++ Y  LKS
Sbjct: 12  IVIRDVWAYNLESEFDLIRGIVEDYPFISMDTEFPGVIYKADLDVLRRGNPNYLYNLLKS 71

Query: 73  NVDLLHLIQIGITLSDSDGNLPDL-GTGDTRFIWEFNFKDFDVARDLHAPNSIELLRRQG 132
           NVD L LIQ+G+TLSD+DGNLPDL G  + R+IWEFNF+DFDV RD HAP+SIELLRR G
Sbjct: 72  NVDALSLIQVGLTLSDADGNLPDLGGQKNRRYIWEFNFRDFDVERDPHAPDSIELLRRHG 131

Query: 133 IDFDRNREEGIDSSRFAELMMSSGLVCNDSVSWVTFHSAYDFGYLVKILTHQKLPNDLEQ 192
           IDF+RNR EG++S RFAELMMSSGL+CN+SVSWVTFHSAYDFGYLVKILT ++LP  L +
Sbjct: 132 IDFERNRREGVESERFAELMMSSGLICNESVSWVTFHSAYDFGYLVKILTRRQLPVALRE 191

Query: 193 FLGVVRLFFGDNVYDMKYLMRFC-QSLYGGLDRIAKTMNVNRAVGKCHQAGSDSLLTWHA 252
           FLG++R FFGD VYD+K++MRFC Q LYGGLDR+A+++ VNRAVGKCHQAGSDSLLTW A
Sbjct: 192 FLGLLRAFFGDRVYDVKHIMRFCEQRLYGGLDRVARSLEVNRAVGKCHQAGSDSLLTWQA 251

Query: 253 FQKMRDTFFVQTEMHKHAGVLYGLEVF 278
           FQ+MRD +FV+    KHAGVLYGLEVF
Sbjct: 252 FQRMRDLYFVEDGAEKHAGVLYGLEVF 278

[Close]

BLAST of CSPI01G15070.1 vs. ExPASy Swiss-Prot
Match: Q9LXM2 (Probable CCR4-associated factor 1 homolog 9 OS=Arabidopsis thaliana OX=3702 GN=CAF1-9 PE=2 SV=1)

HSP 1 Score: 377.9 bits (969), Expect = 1.0e-103
Identity = 183/275 (66.55%), Postives = 221/275 (80.36%), Query Frame = 0


Query: 5   DLNINHKSIQIREVWASNLESEFRLIRDLIDQFPCVSMDTEFPGVIFPQQAKPKSPNHPS 64
           DL  +  ++  REVWA NLESEF LI ++ID +P +SMDTEFPGVIF    +     +P 
Sbjct: 9   DLKPDGVTVVTREVWAENLESEFELISEIIDDYPFISMDTEFPGVIFKSDLR---FTNPD 68

Query: 65  HHYQFLKSNVDLLHLIQIGITLSDSDGNLPDLGTGDTR-FIWEFNFKDFDVARDLHAPNS 124
             Y  LK+NVD L LIQ+G+TLSD +GNLPDLG    R FIWEFNF+DFDVARD HAP+S
Sbjct: 69  DLYTLLKANVDALSLIQVGLTLSDVNGNLPDLGDDLHRGFIWEFNFRDFDVARDAHAPDS 128

Query: 125 IELLRRQGIDFDRNREEGIDSSRFAELMMSSGLVCNDSVSWVTFHSAYDFGYLVKILTHQ 184
           IELLRRQGIDF+RN  +G++S RFAELMMSSGLVCN+ VSWVTFHSAYDFGYL+KILT +
Sbjct: 129 IELLRRQGIDFERNCRDGVESERFAELMMSSGLVCNEEVSWVTFHSAYDFGYLMKILTRR 188

Query: 185 KLPNDLEQFLGVVRLFFGDNVYDMKYLMRFCQ-SLYGGLDRIAKTMNVNRAVGKCHQAGS 244
           +LP  L +F  V+R+ FG+ VYD+K++M+FC+  L+GGLDR+A+T+ VNRAVGKCHQAGS
Sbjct: 189 ELPGALGEFKRVMRVLFGERVYDVKHMMKFCERRLFGGLDRVARTLEVNRAVGKCHQAGS 248

Query: 245 DSLLTWHAFQKMRDTFFVQTEMHKHAGVLYGLEVF 278
           DSLLTWHAFQ+MRD +FVQ    KHAGVLYGLEVF
Sbjct: 249 DSLLTWHAFQRMRDLYFVQDGPEKHAGVLYGLEVF 280

[Close]

BLAST of CSPI01G15070.1 vs. ExPASy Swiss-Prot
Match: Q9SKZ2 (Probable CCR4-associated factor 1 homolog 7 OS=Arabidopsis thaliana OX=3702 GN=CAF1-7 PE=2 SV=2)

HSP 1 Score: 299.3 bits (765), Expect = 4.6e-80
Identity = 147/271 (54.24%), Postives = 202/271 (74.54%), Query Frame = 0


Query: 6   LNINHKSIQIREVWASNLESEFRLIRDLIDQFPCVSMDTEFPGVIFPQQAKPKSPNHPSH 65
           L +   SIQIREVW  NLESE  LIR+++D FP V+MDTEFPG++       K+  +  +
Sbjct: 3   LFLKDDSIQIREVWNDNLESEMALIREVVDDFPFVAMDTEFPGIVCRPVGTFKT--NTEY 62

Query: 66  HYQFLKSNVDLLHLIQIGITLSDSDGNLPDLGTGDTRFIWEFNFKDFDVARDLHAPNSIE 125
           HY+ LK+NV++L +IQ+G+T SD  GNLP  GT +   IW+FNF++FD+  D++A +SIE
Sbjct: 63  HYETLKTNVNILKMIQLGLTFSDEKGNLPTCGTDNKYCIWQFNFREFDLESDIYATDSIE 122

Query: 126 LLRRQGIDFDRNREEGIDSSRFAELMMSSGLVCNDSVSWVTFHSAYDFGYLVKILTHQKL 185
           LLR+ GIDF +N E GIDS RFAEL+MSSG+V N++V WVTFHS YDFGYL+K+LT Q L
Sbjct: 123 LLRQSGIDFVKNNEFGIDSKRFAELLMSSGIVLNENVHWVTFHSGYDFGYLLKLLTCQNL 182

Query: 186 PNDLEQFLGVVRLFFGDNVYDMKYLMRFCQSLYGGLDRIAKTMNVNRAVGKCHQAGSDSL 245
           P     F  ++ ++F   VYD+K+LM+FC SL+GGL+++A+ ++V R VG CHQAGSDSL
Sbjct: 183 PETQTGFFEMISVYF-PRVYDIKHLMKFCNSLHGGLNKLAELLDVER-VGICHQAGSDSL 242

Query: 246 LTWHAFQKMRDTFFVQTEMHKHAGVLYGLEV 277
           LT   F+K+++ FF+ + M K++GVLYGL V
Sbjct: 243 LTSCTFRKLQENFFIGS-MEKYSGVLYGLGV 268

[Close]

BLAST of CSPI01G15070.1 vs. ExPASy Swiss-Prot
Match: Q9SAI2 (Probable CCR4-associated factor 1 homolog 6 OS=Arabidopsis thaliana OX=3702 GN=CAF1-6 PE=1 SV=1)

HSP 1 Score: 292.0 bits (746), Expect = 7.3e-78
Identity = 145/271 (53.51%), Postives = 199/271 (73.43%), Query Frame = 0


Query: 6   LNINHKSIQIREVWASNLESEFRLIRDLIDQFPCVSMDTEFPGVIFPQQAKPKSPNHPSH 65
           L +   SIQIREVW  NL+ E  LIRD++D FP V+MDTEFPG++       KS  +  +
Sbjct: 3   LFLKDDSIQIREVWNDNLQEEMDLIRDVVDDFPYVAMDTEFPGIVVRPVGTFKS--NADY 62

Query: 66  HYQFLKSNVDLLHLIQIGITLSDSDGNLPDLGTGDTRFIWEFNFKDFDVARDLHAPNSIE 125
           HY+ LK+NV++L +IQ+G+T S+  GNLP  GT D   IW+FNF++FD+  D+ A +SIE
Sbjct: 63  HYETLKTNVNILKMIQLGLTFSNEQGNLPTCGT-DKYCIWQFNFREFDLDSDIFALDSIE 122

Query: 126 LLRRQGIDFDRNREEGIDSSRFAELMMSSGLVCNDSVSWVTFHSAYDFGYLVKILTHQKL 185
           LL++ GID  +N  +GIDS RFAEL+MSSG+V N++V WVTFHS YDFGYL+K+LT Q L
Sbjct: 123 LLKQSGIDLAKNTLDGIDSKRFAELLMSSGIVLNENVHWVTFHSGYDFGYLLKLLTCQNL 182

Query: 186 PNDLEQFLGVVRLFFGDNVYDMKYLMRFCQSLYGGLDRIAKTMNVNRAVGKCHQAGSDSL 245
           P+    F  ++ ++F   VYD+K+LM+FC SL+GGL+++A+ + V R VG CHQAGSDSL
Sbjct: 183 PDSQTDFFKLINVYF-PTVYDIKHLMKFCNSLHGGLNKLAELLEVER-VGICHQAGSDSL 242

Query: 246 LTWHAFQKMRDTFFVQTEMHKHAGVLYGLEV 277
           LT   F+K+++ FFV   +HK++GVLYGL V
Sbjct: 243 LTSCTFRKLKENFFV-GPLHKYSGVLYGLGV 267

[Close]

BLAST of CSPI01G15070.1 vs. ExPASy Swiss-Prot
Match: Q9LEU4 (Probable CCR4-associated factor 1 homolog 10 OS=Arabidopsis thaliana OX=3702 GN=CAF1-10 PE=2 SV=1)

HSP 1 Score: 283.1 bits (723), Expect = 3.4e-75
Identity = 145/269 (53.90%), Postives = 191/269 (71.00%), Query Frame = 0


Query: 8   INHKSIQIREVWASNLESEFRLIRDLIDQFPCVSMDTEFPGVIFPQQAKPKSPNHPSHHY 67
           +   SI IREVW  NL  EF LIR+++D+F  ++MDTEFPGV+    A  K  N    +Y
Sbjct: 5   LKEDSIMIREVWDYNLVEEFALIREIVDKFSYIAMDTEFPGVVLKPVATFKYNN--DLNY 64

Query: 68  QFLKSNVDLLHLIQIGITLSDSDGNLPDLGTGDTRFIWEFNFKDFDVARDLHAPNSIELL 127
           + LK NVDLL LIQ+G+T SD +GNLP  GT D   IW+FNF++F++  D++A  SIELL
Sbjct: 65  RTLKENVDLLKLIQVGLTFSDENGNLPTCGT-DKFCIWQFNFREFNIGEDIYASESIELL 124

Query: 128 RRQGIDFDRNREEGIDSSRFAELMMSSGLVCNDSVSWVTFHSAYDFGYLVKILTHQKLPN 187
           R+ GIDF +N E+GID  RF ELMMSSG+V ND++SWVTFH  YDFGYLVK+LT ++LP 
Sbjct: 125 RQCGIDFKKNIEKGIDVVRFGELMMSSGIVLNDAISWVTFHGGYDFGYLVKLLTCKELPL 184

Query: 188 DLEQFLGVVRLFFGDNVYDMKYLMRFCQSLYGGLDRIAKTMNVNRAVGKCHQAGSDSLLT 247
               F  ++ ++F   VYD+K+LM FC  L+GGL+R+A+ M V R VG CHQAGSDSLLT
Sbjct: 185 KQADFFKLLYVYF-PTVYDIKHLMTFCNGLFGGLNRLAELMGVER-VGICHQAGSDSLLT 244

Query: 248 WHAFQKMRDTFFVQTEMHKHAGVLYGLEV 277
             +F+K+++ +F      K+ GVLYGL V
Sbjct: 245 LGSFRKLKERYF-PGSTEKYTGVLYGLGV 267

[Close]

BLAST of CSPI01G15070.1 vs. ExPASy TrEMBL
Match: A0A0A0LYC8 (Poly(A)-specific ribonuclease OS=Cucumis sativus OX=3659 GN=Csa_1G168920 PE=3 SV=1)

HSP 1 Score: 568.5 bits (1464), Expect = 1.5e-158
Identity = 273/277 (98.56%), Postives = 273/277 (98.56%), Query Frame = 0


Query: 1   MLIHDLNINHKSIQIREVWASNLESEFRLIRDLIDQFPCVSMDTEFPGVIFPQQAKPKSP 60
           MLIHDLN NHKSIQIREVWASNLESEFRLIRDLIDQFPCVSMDTEFPGVIFPQQ  PKSP
Sbjct: 1   MLIHDLNTNHKSIQIREVWASNLESEFRLIRDLIDQFPCVSMDTEFPGVIFPQQDIPKSP 60

Query: 61  NHPSHHYQFLKSNVDLLHLIQIGITLSDSDGNLPDLGTGDTRFIWEFNFKDFDVARDLHA 120
           NHPSHHYQFLKSNVDLLHLIQIGITLSDSDGNLPDLGTGDTRFIWEFNFKDFDVARDLHA
Sbjct: 61  NHPSHHYQFLKSNVDLLHLIQIGITLSDSDGNLPDLGTGDTRFIWEFNFKDFDVARDLHA 120

Query: 121 PNSIELLRRQGIDFDRNREEGIDSSRFAELMMSSGLVCNDSVSWVTFHSAYDFGYLVKIL 180
           PNSIELLRRQGIDFDRNREEGIDSSRFAELMMSSGLVCNDSVSWVTFHSAYDFGYLVKIL
Sbjct: 121 PNSIELLRRQGIDFDRNREEGIDSSRFAELMMSSGLVCNDSVSWVTFHSAYDFGYLVKIL 180

Query: 181 THQKLPNDLEQFLGVVRLFFGDNVYDMKYLMRFCQSLYGGLDRIAKTMNVNRAVGKCHQA 240
           THQKLP DLEQFLGVVRLFFGDNVYDMKYLMRFCQSLYGGLDRIAKTMNVNRAVGKCHQA
Sbjct: 181 THQKLPKDLEQFLGVVRLFFGDNVYDMKYLMRFCQSLYGGLDRIAKTMNVNRAVGKCHQA 240

Query: 241 GSDSLLTWHAFQKMRDTFFVQTEMHKHAGVLYGLEVF 278
           GSDSLLTWHAFQKMRDTFFVQTEMHKHAGVLYGLEVF
Sbjct: 241 GSDSLLTWHAFQKMRDTFFVQTEMHKHAGVLYGLEVF 277

[Close]

BLAST of CSPI01G15070.1 vs. ExPASy TrEMBL
Match: A0A5A7T1W0 (Poly(A)-specific ribonuclease OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G001920 PE=3 SV=1)

HSP 1 Score: 536.6 bits (1381), Expect = 6.3e-149
Identity = 257/281 (91.46%), Postives = 269/281 (95.73%), Query Frame = 0


Query: 1   MLIHDLNINHKS----IQIREVWASNLESEFRLIRDLIDQFPCVSMDTEFPGVIFPQQAK 60
           MLIHDLNINH+S    IQIREVWASNL+SEFRLI DLID+FPCVSMDTEFPGVIFPQQ K
Sbjct: 1   MLIHDLNINHQSIHKPIQIREVWASNLDSEFRLISDLIDRFPCVSMDTEFPGVIFPQQTK 60

Query: 61  PKSPNHPSHHYQFLKSNVDLLHLIQIGITLSDSDGNLPDLGTGDTRFIWEFNFKDFDVAR 120
           PK PNHPS+HYQFLKSNVD+LHLIQIGITLSDSDGNLPDLGTGDTRFIWEFNFKDFDVAR
Sbjct: 61  PKFPNHPSYHYQFLKSNVDVLHLIQIGITLSDSDGNLPDLGTGDTRFIWEFNFKDFDVAR 120

Query: 121 DLHAPNSIELLRRQGIDFDRNREEGIDSSRFAELMMSSGLVCNDSVSWVTFHSAYDFGYL 180
           D HAP+SIELLRRQGIDF+RNREEGIDSSRFAELMMSSGLVCN+SVSWVTFHSAYDFGYL
Sbjct: 121 DPHAPDSIELLRRQGIDFERNREEGIDSSRFAELMMSSGLVCNESVSWVTFHSAYDFGYL 180

Query: 181 VKILTHQKLPNDLEQFLGVVRLFFGDNVYDMKYLMRFCQSLYGGLDRIAKTMNVNRAVGK 240
           VKILT QKLP  LE+FLGVVR+FFGDNVYDMKY+MRFCQSLYGGLDRIAKTMNVNRAVGK
Sbjct: 181 VKILTQQKLPKGLEEFLGVVRVFFGDNVYDMKYMMRFCQSLYGGLDRIAKTMNVNRAVGK 240

Query: 241 CHQAGSDSLLTWHAFQKMRDTFFVQTEMHKHAGVLYGLEVF 278
           CHQAGSDSLLTWHAFQKMRDTFFV+ EMHKHAGVLYGLEVF
Sbjct: 241 CHQAGSDSLLTWHAFQKMRDTFFVENEMHKHAGVLYGLEVF 281

[Close]

BLAST of CSPI01G15070.1 vs. ExPASy TrEMBL
Match: A0A1S3CJQ2 (Poly(A)-specific ribonuclease OS=Cucumis melo OX=3656 GN=LOC103501738 PE=3 SV=1)

HSP 1 Score: 536.6 bits (1381), Expect = 6.3e-149
Identity = 257/281 (91.46%), Postives = 269/281 (95.73%), Query Frame = 0


Query: 1   MLIHDLNINHKS----IQIREVWASNLESEFRLIRDLIDQFPCVSMDTEFPGVIFPQQAK 60
           MLIHDLNINH+S    IQIREVWASNL+SEFRLI DLID+FPCVSMDTEFPGVIFPQQ K
Sbjct: 1   MLIHDLNINHQSIHKPIQIREVWASNLDSEFRLISDLIDRFPCVSMDTEFPGVIFPQQTK 60

Query: 61  PKSPNHPSHHYQFLKSNVDLLHLIQIGITLSDSDGNLPDLGTGDTRFIWEFNFKDFDVAR 120
           PK PNHPS+HYQFLKSNVD+LHLIQIGITLSDSDGNLPDLGTGDTRFIWEFNFKDFDVAR
Sbjct: 61  PKFPNHPSYHYQFLKSNVDVLHLIQIGITLSDSDGNLPDLGTGDTRFIWEFNFKDFDVAR 120

Query: 121 DLHAPNSIELLRRQGIDFDRNREEGIDSSRFAELMMSSGLVCNDSVSWVTFHSAYDFGYL 180
           D HAP+SIELLRRQGIDF+RNREEGIDSSRFAELMMSSGLVCN+SVSWVTFHSAYDFGYL
Sbjct: 121 DPHAPDSIELLRRQGIDFERNREEGIDSSRFAELMMSSGLVCNESVSWVTFHSAYDFGYL 180

Query: 181 VKILTHQKLPNDLEQFLGVVRLFFGDNVYDMKYLMRFCQSLYGGLDRIAKTMNVNRAVGK 240
           VKILT QKLP  LE+FLGVVR+FFGDNVYDMKY+MRFCQSLYGGLDRIAKTMNVNRAVGK
Sbjct: 181 VKILTQQKLPKGLEEFLGVVRVFFGDNVYDMKYMMRFCQSLYGGLDRIAKTMNVNRAVGK 240

Query: 241 CHQAGSDSLLTWHAFQKMRDTFFVQTEMHKHAGVLYGLEVF 278
           CHQAGSDSLLTWHAFQKMRDTFFV+ EMHKHAGVLYGLEVF
Sbjct: 241 CHQAGSDSLLTWHAFQKMRDTFFVENEMHKHAGVLYGLEVF 281

[Close]

BLAST of CSPI01G15070.1 vs. ExPASy TrEMBL
Match: A0A6J1CN71 (Poly(A)-specific ribonuclease OS=Momordica charantia OX=3673 GN=LOC111012547 PE=3 SV=1)

HSP 1 Score: 473.4 bits (1217), Expect = 6.5e-130
Identity = 227/277 (81.95%), Postives = 248/277 (89.53%), Query Frame = 0


Query: 1   MLIHDLNINHKSIQIREVWASNLESEFRLIRDLIDQFPCVSMDTEFPGVIFPQQAKP-KS 60
           M++ DL  N K IQIREVWASNLESEF LIRDLIDQFPCVSMDTEFPGV+F   AKP   
Sbjct: 1   MVVQDLKNNSKPIQIREVWASNLESEFELIRDLIDQFPCVSMDTEFPGVVFTDPAKPYHR 60

Query: 61  PNHPSHHYQFLKSNVDLLHLIQIGITLSDSDGNLPDLGTGDTRFIWEFNFKDFDVARDLH 120
            N PS HY+ LKSNVD L+LIQIGITLSDSDGNLPDLGTG +RFIWEFNF DFDVARD+H
Sbjct: 61  QNRPSDHYRLLKSNVDALNLIQIGITLSDSDGNLPDLGTGGSRFIWEFNFMDFDVARDVH 120

Query: 121 APNSIELLRRQGIDFDRNREEGIDSSRFAELMMSSGLVCNDSVSWVTFHSAYDFGYLVKI 180
           AP+SIELLRRQGIDFDRNR EGIDS RFAELMMSSGLVCNDSVSWVTFHSAYDFGYLVK+
Sbjct: 121 APDSIELLRRQGIDFDRNRVEGIDSCRFAELMMSSGLVCNDSVSWVTFHSAYDFGYLVKV 180

Query: 181 LTHQKLPNDLEQFLGVVRLFFGDNVYDMKYLMRFCQSLYGGLDRIAKTMNVNRAVGKCHQ 240
           LT + LP+ LE+FLGV+R+FFGD VYD+KY+MRFC+SLYGGLDRIAK +NV+RAVGKCHQ
Sbjct: 181 LTRRSLPSRLEEFLGVLRVFFGDQVYDVKYMMRFCKSLYGGLDRIAKILNVSRAVGKCHQ 240

Query: 241 AGSDSLLTWHAFQKMRDTFFVQTEMHKHAGVLYGLEV 277
           AGSDSLLTWHAFQKMRDT+FVQ  + KHAGVLYGLEV
Sbjct: 241 AGSDSLLTWHAFQKMRDTYFVQNGLEKHAGVLYGLEV 277

[Close]

BLAST of CSPI01G15070.1 vs. ExPASy TrEMBL
Match: A0A6J1G223 (Poly(A)-specific ribonuclease OS=Cucurbita moschata OX=3662 GN=LOC111449978 PE=3 SV=1)

HSP 1 Score: 464.5 bits (1194), Expect = 3.0e-127
Identity = 223/280 (79.64%), Postives = 249/280 (88.93%), Query Frame = 0


Query: 1   MLIHDLNINH-KSIQIREVWASNLESEFRLIRDLIDQFPCVSMDTEFPGVIFPQQAKPKS 60
           M+ HDLN NH KSI+IREVWASNLESEF+LIRDLID FPCVSMDTEFPG++F     P +
Sbjct: 1   MVSHDLNNNHYKSIKIREVWASNLESEFQLIRDLIDHFPCVSMDTEFPGIVFTAPTAPFN 60

Query: 61  P--NHPSHHYQFLKSNVDLLHLIQIGITLSDSDGNLPDLGTGDTRFIWEFNFKDFDVARD 120
                 S HY+ LKSNVD L+LIQIGITLSDSDGNLPDLGT D+RFIWEFNF DFD+ARD
Sbjct: 61  ALNRSSSDHYKLLKSNVDALNLIQIGITLSDSDGNLPDLGTDDSRFIWEFNFMDFDLARD 120

Query: 121 LHAPNSIELLRRQGIDFDRNREEGIDSSRFAELMMSSGLVCNDSVSWVTFHSAYDFGYLV 180
           ++AP+SI+LLRRQGIDFDRNR EGIDS RFAELMMSSGLVCNDSVSWVTFHSAYDF YL+
Sbjct: 121 IYAPDSIDLLRRQGIDFDRNRVEGIDSCRFAELMMSSGLVCNDSVSWVTFHSAYDFAYLI 180

Query: 181 KILTHQKLPNDLEQFLGVVRLFFGDNVYDMKYLMRFCQSLYGGLDRIAKTMNVNRAVGKC 240
           KILT + LPN L++FLGV+R+ FGDNVYD+KY+MRFC+SLYGGLDRIAK++NVNRAVGKC
Sbjct: 181 KILTQKSLPNRLDEFLGVLRVLFGDNVYDVKYMMRFCKSLYGGLDRIAKSLNVNRAVGKC 240

Query: 241 HQAGSDSLLTWHAFQKMRDTFFVQTEMHKHAGVLYGLEVF 278
           HQAGSDSLLTWHAFQKMRDTFFVQ E+ KHAGVLYGLE F
Sbjct: 241 HQAGSDSLLTWHAFQKMRDTFFVQNEVEKHAGVLYGLEAF 280

[Close]

BLAST of CSPI01G15070.1 vs. NCBI nr
Match: XP_004139505.1 (LOW QUALITY PROTEIN: probable CCR4-associated factor 1 homolog 11 [Cucumis sativus] >XP_031745377.1 LOW QUALITY PROTEIN: probable CCR4-associated factor 1 homolog 11 [Cucumis sativus])

HSP 1 Score: 568.5 bits (1464), Expect = 3.1e-158
Identity = 273/277 (98.56%), Postives = 273/277 (98.56%), Query Frame = 0


Query: 1   MLIHDLNINHKSIQIREVWASNLESEFRLIRDLIDQFPCVSMDTEFPGVIFPQQAKPKSP 60
           MLIHDLN NHKSIQIREVWASNLESEFRLIRDLIDQFPCVSMDTEFPGVIFPQQ  PKSP
Sbjct: 1   MLIHDLNTNHKSIQIREVWASNLESEFRLIRDLIDQFPCVSMDTEFPGVIFPQQDIPKSP 60

Query: 61  NHPSHHYQFLKSNVDLLHLIQIGITLSDSDGNLPDLGTGDTRFIWEFNFKDFDVARDLHA 120
           NHPSHHYQFLKSNVDLLHLIQIGITLSDSDGNLPDLGTGDTRFIWEFNFKDFDVARDLHA
Sbjct: 61  NHPSHHYQFLKSNVDLLHLIQIGITLSDSDGNLPDLGTGDTRFIWEFNFKDFDVARDLHA 120

Query: 121 PNSIELLRRQGIDFDRNREEGIDSSRFAELMMSSGLVCNDSVSWVTFHSAYDFGYLVKIL 180
           PNSIELLRRQGIDFDRNREEGIDSSRFAELMMSSGLVCNDSVSWVTFHSAYDFGYLVKIL
Sbjct: 121 PNSIELLRRQGIDFDRNREEGIDSSRFAELMMSSGLVCNDSVSWVTFHSAYDFGYLVKIL 180

Query: 181 THQKLPNDLEQFLGVVRLFFGDNVYDMKYLMRFCQSLYGGLDRIAKTMNVNRAVGKCHQA 240
           THQKLP DLEQFLGVVRLFFGDNVYDMKYLMRFCQSLYGGLDRIAKTMNVNRAVGKCHQA
Sbjct: 181 THQKLPKDLEQFLGVVRLFFGDNVYDMKYLMRFCQSLYGGLDRIAKTMNVNRAVGKCHQA 240

Query: 241 GSDSLLTWHAFQKMRDTFFVQTEMHKHAGVLYGLEVF 278
           GSDSLLTWHAFQKMRDTFFVQTEMHKHAGVLYGLEVF
Sbjct: 241 GSDSLLTWHAFQKMRDTFFVQTEMHKHAGVLYGLEVF 277

[Close]

BLAST of CSPI01G15070.1 vs. NCBI nr
Match: XP_008463638.1 (PREDICTED: probable CCR4-associated factor 1 homolog 11 [Cucumis melo] >KAA0035571.1 putative CCR4-associated factor 1-like protein 11 [Cucumis melo var. makuwa] >TYK30965.1 putative CCR4-associated factor 1-like protein 11 [Cucumis melo var. makuwa])

HSP 1 Score: 536.6 bits (1381), Expect = 1.3e-148
Identity = 257/281 (91.46%), Postives = 269/281 (95.73%), Query Frame = 0


Query: 1   MLIHDLNINHKS----IQIREVWASNLESEFRLIRDLIDQFPCVSMDTEFPGVIFPQQAK 60
           MLIHDLNINH+S    IQIREVWASNL+SEFRLI DLID+FPCVSMDTEFPGVIFPQQ K
Sbjct: 1   MLIHDLNINHQSIHKPIQIREVWASNLDSEFRLISDLIDRFPCVSMDTEFPGVIFPQQTK 60

Query: 61  PKSPNHPSHHYQFLKSNVDLLHLIQIGITLSDSDGNLPDLGTGDTRFIWEFNFKDFDVAR 120
           PK PNHPS+HYQFLKSNVD+LHLIQIGITLSDSDGNLPDLGTGDTRFIWEFNFKDFDVAR
Sbjct: 61  PKFPNHPSYHYQFLKSNVDVLHLIQIGITLSDSDGNLPDLGTGDTRFIWEFNFKDFDVAR 120

Query: 121 DLHAPNSIELLRRQGIDFDRNREEGIDSSRFAELMMSSGLVCNDSVSWVTFHSAYDFGYL 180
           D HAP+SIELLRRQGIDF+RNREEGIDSSRFAELMMSSGLVCN+SVSWVTFHSAYDFGYL
Sbjct: 121 DPHAPDSIELLRRQGIDFERNREEGIDSSRFAELMMSSGLVCNESVSWVTFHSAYDFGYL 180

Query: 181 VKILTHQKLPNDLEQFLGVVRLFFGDNVYDMKYLMRFCQSLYGGLDRIAKTMNVNRAVGK 240
           VKILT QKLP  LE+FLGVVR+FFGDNVYDMKY+MRFCQSLYGGLDRIAKTMNVNRAVGK
Sbjct: 181 VKILTQQKLPKGLEEFLGVVRVFFGDNVYDMKYMMRFCQSLYGGLDRIAKTMNVNRAVGK 240

Query: 241 CHQAGSDSLLTWHAFQKMRDTFFVQTEMHKHAGVLYGLEVF 278
           CHQAGSDSLLTWHAFQKMRDTFFV+ EMHKHAGVLYGLEVF
Sbjct: 241 CHQAGSDSLLTWHAFQKMRDTFFVENEMHKHAGVLYGLEVF 281

[Close]

BLAST of CSPI01G15070.1 vs. NCBI nr
Match: XP_038877224.1 (probable CCR4-associated factor 1 homolog 11 [Benincasa hispida])

HSP 1 Score: 495.0 bits (1273), Expect = 4.3e-136
Identity = 236/278 (84.89%), Postives = 259/278 (93.17%), Query Frame = 0


Query: 1   MLIHDLNINHKSIQIREVWASNLESEFRLIRDLIDQFPCVSMDTEFPGVIFPQQAKP-KS 60
           M+IHDLNIN+KSI+IREVWASNLESEF+LIRDLIDQFPCVSMDTEFPG++F   A+P   
Sbjct: 1   MVIHDLNINNKSIKIREVWASNLESEFQLIRDLIDQFPCVSMDTEFPGIVFADPARPFNF 60

Query: 61  PNHPSHHYQFLKSNVDLLHLIQIGITLSDSDGNLPDLGTGDTRFIWEFNFKDFDVARDLH 120
            + PS HY+ LKSNVD LHLIQIGITLSDSDGNLPDLGT DTRFIWEFNF DFDVARDLH
Sbjct: 61  QSQPSDHYRLLKSNVDALHLIQIGITLSDSDGNLPDLGTDDTRFIWEFNFMDFDVARDLH 120

Query: 121 APNSIELLRRQGIDFDRNREEGIDSSRFAELMMSSGLVCNDSVSWVTFHSAYDFGYLVKI 180
           AP+SIELLRRQGIDF+RNR EGIDSSRFAELMMSSGLVCN+SVSWVTFHSAYDFGYLVKI
Sbjct: 121 APDSIELLRRQGIDFNRNRVEGIDSSRFAELMMSSGLVCNNSVSWVTFHSAYDFGYLVKI 180

Query: 181 LTHQKLPNDLEQFLGVVRLFFGDNVYDMKYLMRFCQSLYGGLDRIAKTMNVNRAVGKCHQ 240
           LT ++LP+ +++FLGV+R+FFGDNVYDMKYLMRFC+SLYGGLDRIAK +NVNRAVGKCHQ
Sbjct: 181 LTQRRLPDRVDEFLGVLRVFFGDNVYDMKYLMRFCESLYGGLDRIAKILNVNRAVGKCHQ 240

Query: 241 AGSDSLLTWHAFQKMRDTFFVQTEMHKHAGVLYGLEVF 278
           AGSDSLLTWHAFQKMRDT+FVQ EM KHAGVLYGLEVF
Sbjct: 241 AGSDSLLTWHAFQKMRDTYFVQNEMQKHAGVLYGLEVF 278

[Close]

BLAST of CSPI01G15070.1 vs. NCBI nr
Match: XP_022142408.1 (probable CCR4-associated factor 1 homolog 11 [Momordica charantia])

HSP 1 Score: 473.4 bits (1217), Expect = 1.3e-129
Identity = 227/277 (81.95%), Postives = 248/277 (89.53%), Query Frame = 0


Query: 1   MLIHDLNINHKSIQIREVWASNLESEFRLIRDLIDQFPCVSMDTEFPGVIFPQQAKP-KS 60
           M++ DL  N K IQIREVWASNLESEF LIRDLIDQFPCVSMDTEFPGV+F   AKP   
Sbjct: 1   MVVQDLKNNSKPIQIREVWASNLESEFELIRDLIDQFPCVSMDTEFPGVVFTDPAKPYHR 60

Query: 61  PNHPSHHYQFLKSNVDLLHLIQIGITLSDSDGNLPDLGTGDTRFIWEFNFKDFDVARDLH 120
            N PS HY+ LKSNVD L+LIQIGITLSDSDGNLPDLGTG +RFIWEFNF DFDVARD+H
Sbjct: 61  QNRPSDHYRLLKSNVDALNLIQIGITLSDSDGNLPDLGTGGSRFIWEFNFMDFDVARDVH 120

Query: 121 APNSIELLRRQGIDFDRNREEGIDSSRFAELMMSSGLVCNDSVSWVTFHSAYDFGYLVKI 180
           AP+SIELLRRQGIDFDRNR EGIDS RFAELMMSSGLVCNDSVSWVTFHSAYDFGYLVK+
Sbjct: 121 APDSIELLRRQGIDFDRNRVEGIDSCRFAELMMSSGLVCNDSVSWVTFHSAYDFGYLVKV 180

Query: 181 LTHQKLPNDLEQFLGVVRLFFGDNVYDMKYLMRFCQSLYGGLDRIAKTMNVNRAVGKCHQ 240
           LT + LP+ LE+FLGV+R+FFGD VYD+KY+MRFC+SLYGGLDRIAK +NV+RAVGKCHQ
Sbjct: 181 LTRRSLPSRLEEFLGVLRVFFGDQVYDVKYMMRFCKSLYGGLDRIAKILNVSRAVGKCHQ 240

Query: 241 AGSDSLLTWHAFQKMRDTFFVQTEMHKHAGVLYGLEV 277
           AGSDSLLTWHAFQKMRDT+FVQ  + KHAGVLYGLEV
Sbjct: 241 AGSDSLLTWHAFQKMRDTYFVQNGLEKHAGVLYGLEV 277

[Close]

BLAST of CSPI01G15070.1 vs. NCBI nr
Match: XP_022945861.1 (probable CCR4-associated factor 1 homolog 11 [Cucurbita moschata] >KAG6573637.1 putative CCR4-associated factor 1-like 11, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 464.5 bits (1194), Expect = 6.3e-127
Identity = 223/280 (79.64%), Postives = 249/280 (88.93%), Query Frame = 0


Query: 1   MLIHDLNINH-KSIQIREVWASNLESEFRLIRDLIDQFPCVSMDTEFPGVIFPQQAKPKS 60
           M+ HDLN NH KSI+IREVWASNLESEF+LIRDLID FPCVSMDTEFPG++F     P +
Sbjct: 1   MVSHDLNNNHYKSIKIREVWASNLESEFQLIRDLIDHFPCVSMDTEFPGIVFTAPTAPFN 60

Query: 61  P--NHPSHHYQFLKSNVDLLHLIQIGITLSDSDGNLPDLGTGDTRFIWEFNFKDFDVARD 120
                 S HY+ LKSNVD L+LIQIGITLSDSDGNLPDLGT D+RFIWEFNF DFD+ARD
Sbjct: 61  ALNRSSSDHYKLLKSNVDALNLIQIGITLSDSDGNLPDLGTDDSRFIWEFNFMDFDLARD 120

Query: 121 LHAPNSIELLRRQGIDFDRNREEGIDSSRFAELMMSSGLVCNDSVSWVTFHSAYDFGYLV 180
           ++AP+SI+LLRRQGIDFDRNR EGIDS RFAELMMSSGLVCNDSVSWVTFHSAYDF YL+
Sbjct: 121 IYAPDSIDLLRRQGIDFDRNRVEGIDSCRFAELMMSSGLVCNDSVSWVTFHSAYDFAYLI 180

Query: 181 KILTHQKLPNDLEQFLGVVRLFFGDNVYDMKYLMRFCQSLYGGLDRIAKTMNVNRAVGKC 240
           KILT + LPN L++FLGV+R+ FGDNVYD+KY+MRFC+SLYGGLDRIAK++NVNRAVGKC
Sbjct: 181 KILTQKSLPNRLDEFLGVLRVLFGDNVYDVKYMMRFCKSLYGGLDRIAKSLNVNRAVGKC 240

Query: 241 HQAGSDSLLTWHAFQKMRDTFFVQTEMHKHAGVLYGLEVF 278
           HQAGSDSLLTWHAFQKMRDTFFVQ E+ KHAGVLYGLE F
Sbjct: 241 HQAGSDSLLTWHAFQKMRDTFFVQNEVEKHAGVLYGLEAF 280

[Close]

BLAST of CSPI01G15070.1 vs. TAIR 10
Match: AT5G22250.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 390.2 bits (1001), Expect = 1.4e-108
Identity = 185/267 (69.29%), Postives = 222/267 (83.15%), Query Frame = 0


Query: 13  IQIREVWASNLESEFRLIRDLIDQFPCVSMDTEFPGVIFPQQAKPKSPNHPSHHYQFLKS 72
           I IR+VWA NLESEF LIR +++ +P +SMDTEFPGVI+          +P++ Y  LKS
Sbjct: 12  IVIRDVWAYNLESEFDLIRGIVEDYPFISMDTEFPGVIYKADLDVLRRGNPNYLYNLLKS 71

Query: 73  NVDLLHLIQIGITLSDSDGNLPDL-GTGDTRFIWEFNFKDFDVARDLHAPNSIELLRRQG 132
           NVD L LIQ+G+TLSD+DGNLPDL G  + R+IWEFNF+DFDV RD HAP+SIELLRR G
Sbjct: 72  NVDALSLIQVGLTLSDADGNLPDLGGQKNRRYIWEFNFRDFDVERDPHAPDSIELLRRHG 131

Query: 133 IDFDRNREEGIDSSRFAELMMSSGLVCNDSVSWVTFHSAYDFGYLVKILTHQKLPNDLEQ 192
           IDF+RNR EG++S RFAELMMSSGL+CN+SVSWVTFHSAYDFGYLVKILT ++LP  L +
Sbjct: 132 IDFERNRREGVESERFAELMMSSGLICNESVSWVTFHSAYDFGYLVKILTRRQLPVALRE 191

Query: 193 FLGVVRLFFGDNVYDMKYLMRFC-QSLYGGLDRIAKTMNVNRAVGKCHQAGSDSLLTWHA 252
           FLG++R FFGD VYD+K++MRFC Q LYGGLDR+A+++ VNRAVGKCHQAGSDSLLTW A
Sbjct: 192 FLGLLRAFFGDRVYDVKHIMRFCEQRLYGGLDRVARSLEVNRAVGKCHQAGSDSLLTWQA 251

Query: 253 FQKMRDTFFVQTEMHKHAGVLYGLEVF 278
           FQ+MRD +FV+    KHAGVLYGLEVF
Sbjct: 252 FQRMRDLYFVEDGAEKHAGVLYGLEVF 278

[Close]

BLAST of CSPI01G15070.1 vs. TAIR 10
Match: AT3G44260.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 377.9 bits (969), Expect = 7.2e-105
Identity = 183/275 (66.55%), Postives = 221/275 (80.36%), Query Frame = 0


Query: 5   DLNINHKSIQIREVWASNLESEFRLIRDLIDQFPCVSMDTEFPGVIFPQQAKPKSPNHPS 64
           DL  +  ++  REVWA NLESEF LI ++ID +P +SMDTEFPGVIF    +     +P 
Sbjct: 9   DLKPDGVTVVTREVWAENLESEFELISEIIDDYPFISMDTEFPGVIFKSDLR---FTNPD 68

Query: 65  HHYQFLKSNVDLLHLIQIGITLSDSDGNLPDLGTGDTR-FIWEFNFKDFDVARDLHAPNS 124
             Y  LK+NVD L LIQ+G+TLSD +GNLPDLG    R FIWEFNF+DFDVARD HAP+S
Sbjct: 69  DLYTLLKANVDALSLIQVGLTLSDVNGNLPDLGDDLHRGFIWEFNFRDFDVARDAHAPDS 128

Query: 125 IELLRRQGIDFDRNREEGIDSSRFAELMMSSGLVCNDSVSWVTFHSAYDFGYLVKILTHQ 184
           IELLRRQGIDF+RN  +G++S RFAELMMSSGLVCN+ VSWVTFHSAYDFGYL+KILT +
Sbjct: 129 IELLRRQGIDFERNCRDGVESERFAELMMSSGLVCNEEVSWVTFHSAYDFGYLMKILTRR 188

Query: 185 KLPNDLEQFLGVVRLFFGDNVYDMKYLMRFCQ-SLYGGLDRIAKTMNVNRAVGKCHQAGS 244
           +LP  L +F  V+R+ FG+ VYD+K++M+FC+  L+GGLDR+A+T+ VNRAVGKCHQAGS
Sbjct: 189 ELPGALGEFKRVMRVLFGERVYDVKHMMKFCERRLFGGLDRVARTLEVNRAVGKCHQAGS 248

Query: 245 DSLLTWHAFQKMRDTFFVQTEMHKHAGVLYGLEVF 278
           DSLLTWHAFQ+MRD +FVQ    KHAGVLYGLEVF
Sbjct: 249 DSLLTWHAFQRMRDLYFVQDGPEKHAGVLYGLEVF 280

[Close]

BLAST of CSPI01G15070.1 vs. TAIR 10
Match: AT2G32070.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 299.3 bits (765), Expect = 3.2e-81
Identity = 147/271 (54.24%), Postives = 202/271 (74.54%), Query Frame = 0


Query: 6   LNINHKSIQIREVWASNLESEFRLIRDLIDQFPCVSMDTEFPGVIFPQQAKPKSPNHPSH 65
           L +   SIQIREVW  NLESE  LIR+++D FP V+MDTEFPG++       K+  +  +
Sbjct: 3   LFLKDDSIQIREVWNDNLESEMALIREVVDDFPFVAMDTEFPGIVCRPVGTFKT--NTEY 62

Query: 66  HYQFLKSNVDLLHLIQIGITLSDSDGNLPDLGTGDTRFIWEFNFKDFDVARDLHAPNSIE 125
           HY+ LK+NV++L +IQ+G+T SD  GNLP  GT +   IW+FNF++FD+  D++A +SIE
Sbjct: 63  HYETLKTNVNILKMIQLGLTFSDEKGNLPTCGTDNKYCIWQFNFREFDLESDIYATDSIE 122

Query: 126 LLRRQGIDFDRNREEGIDSSRFAELMMSSGLVCNDSVSWVTFHSAYDFGYLVKILTHQKL 185
           LLR+ GIDF +N E GIDS RFAEL+MSSG+V N++V WVTFHS YDFGYL+K+LT Q L
Sbjct: 123 LLRQSGIDFVKNNEFGIDSKRFAELLMSSGIVLNENVHWVTFHSGYDFGYLLKLLTCQNL 182

Query: 186 PNDLEQFLGVVRLFFGDNVYDMKYLMRFCQSLYGGLDRIAKTMNVNRAVGKCHQAGSDSL 245
           P     F  ++ ++F   VYD+K+LM+FC SL+GGL+++A+ ++V R VG CHQAGSDSL
Sbjct: 183 PETQTGFFEMISVYF-PRVYDIKHLMKFCNSLHGGLNKLAELLDVER-VGICHQAGSDSL 242

Query: 246 LTWHAFQKMRDTFFVQTEMHKHAGVLYGLEV 277
           LT   F+K+++ FF+ + M K++GVLYGL V
Sbjct: 243 LTSCTFRKLQENFFIGS-MEKYSGVLYGLGV 268

[Close]

BLAST of CSPI01G15070.1 vs. TAIR 10
Match: AT1G80780.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 292.0 bits (746), Expect = 5.2e-79
Identity = 145/271 (53.51%), Postives = 199/271 (73.43%), Query Frame = 0


Query: 6   LNINHKSIQIREVWASNLESEFRLIRDLIDQFPCVSMDTEFPGVIFPQQAKPKSPNHPSH 65
           L +   SIQIREVW  NL+ E  LIRD++D FP V+MDTEFPG++       KS  +  +
Sbjct: 3   LFLKDDSIQIREVWNDNLQEEMDLIRDVVDDFPYVAMDTEFPGIVVRPVGTFKS--NADY 62

Query: 66  HYQFLKSNVDLLHLIQIGITLSDSDGNLPDLGTGDTRFIWEFNFKDFDVARDLHAPNSIE 125
           HY+ LK+NV++L +IQ+G+T S+  GNLP  GT D   IW+FNF++FD+  D+ A +SIE
Sbjct: 63  HYETLKTNVNILKMIQLGLTFSNEQGNLPTCGT-DKYCIWQFNFREFDLDSDIFALDSIE 122

Query: 126 LLRRQGIDFDRNREEGIDSSRFAELMMSSGLVCNDSVSWVTFHSAYDFGYLVKILTHQKL 185
           LL++ GID  +N  +GIDS RFAEL+MSSG+V N++V WVTFHS YDFGYL+K+LT Q L
Sbjct: 123 LLKQSGIDLAKNTLDGIDSKRFAELLMSSGIVLNENVHWVTFHSGYDFGYLLKLLTCQNL 182

Query: 186 PNDLEQFLGVVRLFFGDNVYDMKYLMRFCQSLYGGLDRIAKTMNVNRAVGKCHQAGSDSL 245
           P+    F  ++ ++F   VYD+K+LM+FC SL+GGL+++A+ + V R VG CHQAGSDSL
Sbjct: 183 PDSQTDFFKLINVYF-PTVYDIKHLMKFCNSLHGGLNKLAELLEVER-VGICHQAGSDSL 242

Query: 246 LTWHAFQKMRDTFFVQTEMHKHAGVLYGLEV 277
           LT   F+K+++ FFV   +HK++GVLYGL V
Sbjct: 243 LTSCTFRKLKENFFV-GPLHKYSGVLYGLGV 267

[Close]

BLAST of CSPI01G15070.1 vs. TAIR 10
Match: AT1G80780.2 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 292.0 bits (746), Expect = 5.2e-79
Identity = 145/271 (53.51%), Postives = 199/271 (73.43%), Query Frame = 0


Query: 6   LNINHKSIQIREVWASNLESEFRLIRDLIDQFPCVSMDTEFPGVIFPQQAKPKSPNHPSH 65
           L +   SIQIREVW  NL+ E  LIRD++D FP V+MDTEFPG++       KS  +  +
Sbjct: 3   LFLKDDSIQIREVWNDNLQEEMDLIRDVVDDFPYVAMDTEFPGIVVRPVGTFKS--NADY 62

Query: 66  HYQFLKSNVDLLHLIQIGITLSDSDGNLPDLGTGDTRFIWEFNFKDFDVARDLHAPNSIE 125
           HY+ LK+NV++L +IQ+G+T S+  GNLP  GT D   IW+FNF++FD+  D+ A +SIE
Sbjct: 63  HYETLKTNVNILKMIQLGLTFSNEQGNLPTCGT-DKYCIWQFNFREFDLDSDIFALDSIE 122

Query: 126 LLRRQGIDFDRNREEGIDSSRFAELMMSSGLVCNDSVSWVTFHSAYDFGYLVKILTHQKL 185
           LL++ GID  +N  +GIDS RFAEL+MSSG+V N++V WVTFHS YDFGYL+K+LT Q L
Sbjct: 123 LLKQSGIDLAKNTLDGIDSKRFAELLMSSGIVLNENVHWVTFHSGYDFGYLLKLLTCQNL 182

Query: 186 PNDLEQFLGVVRLFFGDNVYDMKYLMRFCQSLYGGLDRIAKTMNVNRAVGKCHQAGSDSL 245
           P+    F  ++ ++F   VYD+K+LM+FC SL+GGL+++A+ + V R VG CHQAGSDSL
Sbjct: 183 PDSQTDFFKLINVYF-PTVYDIKHLMKFCNSLHGGLNKLAELLEVER-VGICHQAGSDSL 242

Query: 246 LTWHAFQKMRDTFFVQTEMHKHAGVLYGLEV 277
           LT   F+K+++ FFV   +HK++GVLYGL V
Sbjct: 243 LTSCTFRKLKENFFV-GPLHKYSGVLYGLGV 267

The following BLAST results are available for this feature:

BLAST of CSPI01G15070.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FMS6	2.0e-107	69.29	Probable CCR4-associated factor 1 homolog 11 OS=Arabidopsis thaliana OX=3702 GN=...	[more]
Q9LXM2	1.0e-103	66.55	Probable CCR4-associated factor 1 homolog 9 OS=Arabidopsis thaliana OX=3702 GN=C...	[more]
Q9SKZ2	4.6e-80	54.24	Probable CCR4-associated factor 1 homolog 7 OS=Arabidopsis thaliana OX=3702 GN=C...	[more]
Q9SAI2	7.3e-78	53.51	Probable CCR4-associated factor 1 homolog 6 OS=Arabidopsis thaliana OX=3702 GN=C...	[more]
Q9LEU4	3.4e-75	53.90	Probable CCR4-associated factor 1 homolog 10 OS=Arabidopsis thaliana OX=3702 GN=...	[more]

BLAST of CSPI01G15070.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LYC8	1.5e-158	98.56	Poly(A)-specific ribonuclease OS=Cucumis sativus OX=3659 GN=Csa_1G168920 PE=3 SV...	[more]
A0A5A7T1W0	6.3e-149	91.46	Poly(A)-specific ribonuclease OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc...	[more]
A0A1S3CJQ2	6.3e-149	91.46	Poly(A)-specific ribonuclease OS=Cucumis melo OX=3656 GN=LOC103501738 PE=3 SV=1	[more]
A0A6J1CN71	6.5e-130	81.95	Poly(A)-specific ribonuclease OS=Momordica charantia OX=3673 GN=LOC111012547 PE=...	[more]
A0A6J1G223	3.0e-127	79.64	Poly(A)-specific ribonuclease OS=Cucurbita moschata OX=3662 GN=LOC111449978 PE=3...	[more]

BLAST of CSPI01G15070.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004139505.1	3.1e-158	98.56	LOW QUALITY PROTEIN: probable CCR4-associated factor 1 homolog 11 [Cucumis sativ...	[more]
XP_008463638.1	1.3e-148	91.46	PREDICTED: probable CCR4-associated factor 1 homolog 11 [Cucumis melo] >KAA00355...	[more]
XP_038877224.1	4.3e-136	84.89	probable CCR4-associated factor 1 homolog 11 [Benincasa hispida]	[more]
XP_022142408.1	1.3e-129	81.95	probable CCR4-associated factor 1 homolog 11 [Momordica charantia]	[more]
XP_022945861.1	6.3e-127	79.64	probable CCR4-associated factor 1 homolog 11 [Cucurbita moschata] >KAG6573637.1 ...	[more]

BLAST of CSPI01G15070.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G22250.1	1.4e-108	69.29	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT3G44260.1	7.2e-105	66.55	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT2G32070.1	3.2e-81	54.24	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT1G80780.1	5.2e-79	53.51	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT1G80780.2	5.2e-79	53.51	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 4..276 e-value: 3.0E-97 score: 327.0
IPR006941	Ribonuclease CAF1	PFAM	PF04857	CAF1	coord: 18..142 e-value: 2.6E-9 score: 36.8
IPR039637	CCR4-NOT transcription complex subunit 7/8/Pop2	PANTHER	PTHR10797	CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT	coord: 8..276
None	No IPR available	PANTHER	PTHR10797:SF10	CCR4-ASSOCIATED FACTOR 1 HOMOLOG 11-RELATED	coord: 8..276
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 15..274

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CSPI01G15070	CSPI01G15070	gene

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI01G15070.1.utr3p1	CSPI01G15070.1.utr3p1	three_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI01G15070.1.cds1	CSPI01G15070.1.cds1	CDS

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI01G15070.1.utr5p1	CSPI01G15070.1.utr5p1	five_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CSPI01G15070.1	CSPI01G15070.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043928	exonucleolytic catabolism of deadenylated mRNA
biological_process	GO:0090503	RNA phosphodiester bond hydrolysis, exonucleolytic
cellular_component	GO:0030014	CCR4-NOT complex
cellular_component	GO:0030015	CCR4-NOT core complex
cellular_component	GO:0005634	nucleus
cellular_component	GO:0000932	P-body
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004535	poly(A)-specific ribonuclease activity
molecular_function	GO:0016740	transferase activity