CSPI01G00490.1 (mRNA) Cucumber (PI 183967) v1

Overview


Name	CSPI01G00490.1
Type	mRNA
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	mediator of RNA polymerase II transcription subunit 30
Location	Chr1: 261464 .. 263256 (-)
Sequence length	952
RNA-Seq Expression	CSPI01G00490.1
Synteny	CSPI01G00490.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CTGGGGTTTCTCGATTACTAATGAGAGAGTTAGGGTTTCGTGAACATCACAAACTCAAGTGTAATTGAAAGTTGGAATTGGGAATTTTGAAGATTCAGAACTCTCTCTCTTTACAAATTCGGTGCATTTTCGAATACATTATGGAAGAGAAATCGACTAATGTTTTGGCTCTTCCCCAGACAACTCAAGAGCTTGCAATGGAGGGCATCAAGCATCTTGAAGAAACCATAGAATCCGCTTTCCAGATTCTCTACTCCATGAATGACGAGCTCTGTAATCCCACATTATGGTCCACCACTTCTTCAACGGCAACCACGGCTACTTCTGGTTTAACAATTGCCAGCCCAAGTGGTCCATCTTCGCATTCTGCTAATGGAGTTGTAAATGGTGATGCTAGTTCCGAAAGCACTAGCCATCACACCGATTCCAGCGGCGGCGGAGGCAGTGGTAGTGGGGGAGCCCTTGAGGAGGCACGAGTTCGCTACAAGAACTCAGTTCTTGCACTTCGTGCTATTCTTGCTGCAATCCCCAATTCTCAGAAGGTGAAATTACTGACTGATCTTCCATTTATCCATCTAGTTCAATAGCCAATGTTTATCATTTCGAATTTGATTTTTATCTGGGAAAATTGAGATCGATTTGGAGATGTTGGGTTCTCCCCTATGGTACGAAATGCTATAAGTGTCGGAACATCGAATCTTGGTATCCATTTCTGACGAATTCCTTAAATTCTTCCTGAATTTGATAGATTGCAGATGCTACATTTGATCTGGTTTTCATTTTCACTGTTGCTAATTGATGCGTGTACTTACTCCATGTACTTGTAATTTGATAGATTGCTAACTGCATATACTTGTTCTAAACCTATGCTGATTCAATGATTTTAAGTAGAGCTTCGTGTTTATTGATAGGCAAAAGCATACGAATCGGGTACGAGTGGTCATGCATCGCCTGCAGACCAATCTGAAATTGATAAGTTGGAACAACACCTCTCTAATCTCAGAAAGGTATTTTTGTTCTCTTTAGCTTGTGAATTCATGTCAGTAATTTATTTTTTCTTCAATACTTTATGGATGTGGCGTGAACACTTCAATGGTTTCTGGAAATTATTTACTTATTAAGATTTTGGAGTCTACTTAGCTTCAACAACGCAGTACTGTCATTTTTCGATATATCTGTGTAGAATTTACTTAAAAGTTAAAATATATACAATGAAACTGAGAAGGTAACGTCTCTACAAATTTCAAGATAACAGTTCTGTTTTCTTCTTATTTGTGGTATAGTATATCCAAGGAAAGATTTTGAAACTAAACGGACAATAGAGATAATCAAATATTGAAACTTTATAATATTTTAGAGGAATACGTGATAGGTATTATCGATAGTATGCTTTTTCTTTAGCCTTTAAGGTCCTAGAAATGACATTTTTTTCCCTTCAACTACTATCTTACAAGGGTCCTATCTTCGGTTTCTTTGCAGGAACTTGTGGTTAAAAATTTGCATATCAAGCTTCTGATAGATCAACTACGAGATCTTGTTGCTGATGTATCTACATGGCAAAGTCCTTGTTCTGTGTGAAAAAACGTGCTAGCTTTGACTTTAGAGTCTCCTCGCTAATTGAGCTGTTGAAGTTTATAATTGCGATGTTCTGTTTCTTGAAAAGATGAGTTGTTATTAGAACGTTTCTTTGCATCTCTTCATTGAAAGTGGTGGCTATACAAATTTGCCATTGCCTATAAATTGTCAATATAGAGTAAGGCTCTGATAAATCAAGTGCCAAAGGAATTAAAGGT

mRNA sequence

CTGGGGTTTCTCGATTACTAATGAGAGAGTTAGGGTTTCGTGAACATCACAAACTCAAGTGTAATTGAAAGTTGGAATTGGGAATTTTGAAGATTCAGAACTCTCTCTCTTTACAAATTCGGTGCATTTTCGAATACATTATGGAAGAGAAATCGACTAATGTTTTGGCTCTTCCCCAGACAACTCAAGAGCTTGCAATGGAGGGCATCAAGCATCTTGAAGAAACCATAGAATCCGCTTTCCAGATTCTCTACTCCATGAATGACGAGCTCTGTAATCCCACATTATGGTCCACCACTTCTTCAACGGCAACCACGGCTACTTCTGGTTTAACAATTGCCAGCCCAAGTGGTCCATCTTCGCATTCTGCTAATGGAGTTGTAAATGGTGATGCTAGTTCCGAAAGCACTAGCCATCACACCGATTCCAGCGGCGGCGGAGGCAGTGGTAGTGGGGGAGCCCTTGAGGAGGCACGAGTTCGCTACAAGAACTCAGTTCTTGCACTTCGTGCTATTCTTGCTGCAATCCCCAATTCTCAGAAGGCAAAAGCATACGAATCGGGTACGAGTGGTCATGCATCGCCTGCAGACCAATCTGAAATTGATAAGTTGGAACAACACCTCTCTAATCTCAGAAAGGAACTTGTGGTTAAAAATTTGCATATCAAGCTTCTGATAGATCAACTACGAGATCTTGTTGCTGATGTATCTACATGGCAAAGTCCTTGTTCTGTGTGAAAAAACGTGCTAGCTTTGACTTTAGAGTCTCCTCGCTAATTGAGCTGTTGAAGTTTATAATTGCGATGTTCTGTTTCTTGAAAAGATGAGTTGTTATTAGAACGTTTCTTTGCATCTCTTCATTGAAAGTGGTGGCTATACAAATTTGCCATTGCCTATAAATTGTCAATATAGAGTAAGGCTCTGATAAATCAAGTGCCAAAGGAATTAAAGGT

Coding sequence (CDS)

ATGGAAGAGAAATCGACTAATGTTTTGGCTCTTCCCCAGACAACTCAAGAGCTTGCAATGGAGGGCATCAAGCATCTTGAAGAAACCATAGAATCCGCTTTCCAGATTCTCTACTCCATGAATGACGAGCTCTGTAATCCCACATTATGGTCCACCACTTCTTCAACGGCAACCACGGCTACTTCTGGTTTAACAATTGCCAGCCCAAGTGGTCCATCTTCGCATTCTGCTAATGGAGTTGTAAATGGTGATGCTAGTTCCGAAAGCACTAGCCATCACACCGATTCCAGCGGCGGCGGAGGCAGTGGTAGTGGGGGAGCCCTTGAGGAGGCACGAGTTCGCTACAAGAACTCAGTTCTTGCACTTCGTGCTATTCTTGCTGCAATCCCCAATTCTCAGAAGGCAAAAGCATACGAATCGGGTACGAGTGGTCATGCATCGCCTGCAGACCAATCTGAAATTGATAAGTTGGAACAACACCTCTCTAATCTCAGAAAGGAACTTGTGGTTAAAAATTTGCATATCAAGCTTCTGATAGATCAACTACGAGATCTTGTTGCTGATGTATCTACATGGCAAAGTCCTTGTTCTGTGTGA

Protein sequence

MEEKSTNVLALPQTTQELAMEGIKHLEETIESAFQILYSMNDELCNPTLWSTTSSTATTATSGLTIASPSGPSSHSANGVVNGDASSESTSHHTDSSGGGGSGSGGALEEARVRYKNSVLALRAILAAIPNSQKAKAYESGTSGHASPADQSEIDKLEQHLSNLRKELVVKNLHIKLLIDQLRDLVADVSTWQSPCSV*

Homology

[Close]

BLAST of CSPI01G00490.1 vs. ExPASy Swiss-Prot
Match: Q9FMV4 (Mediator of RNA polymerase II transcription subunit 30 OS=Arabidopsis thaliana OX=3702 GN=MED30 PE=1 SV=1)

HSP 1 Score: 183.0 bits (463), Expect = 3.4e-45
Identity = 105/188 (55.85%), Postives = 142/188 (75.53%), Query Frame = 0


Query: 14  TTQELAMEGIKHLEETIESAFQILYSMNDELCNPTLWSTTSSTATTATSGLTIASPSGPS 73
           TTQELAMEG K LEETIE+AFQI+ +MNDELCNP+LWST+++ ++ AT+          +
Sbjct: 13  TTQELAMEGEKQLEETIEAAFQIISAMNDELCNPSLWSTSATPSSAATT----------T 72

Query: 74  SHSANGVVNGDASS-ESTSHHTDSSGGGGSGSGG--ALEEARVRYKNSVLALRAILAAIP 133
             + + +V+ DA++ + TSHH++S+GGGG G  G   L+EA +RYKNSV +LRA+LAAIP
Sbjct: 73  GSNGSALVSADAAAIDGTSHHSESAGGGGGGGSGNSVLDEASLRYKNSVTSLRAVLAAIP 132

Query: 134 NSQKAKAYESGTSGHASPADQSEIDKLEQHLSNLRKELVVKNLHIKLLIDQLRDLVADVS 193
           NSQKAKA E   +G  SP  + EI+KLE+   +LR E+  KN+H+K LID+LR+L+AD+S
Sbjct: 133 NSQKAKASEM-QNGLGSPESEDEIEKLEEQALSLRMEIAKKNVHVKELIDKLRELIADIS 189

Query: 194 TWQSPCSV 199
           TWQSPCSV
Sbjct: 193 TWQSPCSV 189

[Close]

BLAST of CSPI01G00490.1 vs. ExPASy TrEMBL
Match: A0A0A0LNW4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G001500 PE=4 SV=1)

HSP 1 Score: 365.9 bits (938), Expect = 1.0e-97
Identity = 198/198 (100.00%), Postives = 198/198 (100.00%), Query Frame = 0


Query: 1   MEEKSTNVLALPQTTQELAMEGIKHLEETIESAFQILYSMNDELCNPTLWSTTSSTATTA 60
           MEEKSTNVLALPQTTQELAMEGIKHLEETIESAFQILYSMNDELCNPTLWSTTSSTATTA
Sbjct: 1   MEEKSTNVLALPQTTQELAMEGIKHLEETIESAFQILYSMNDELCNPTLWSTTSSTATTA 60

Query: 61  TSGLTIASPSGPSSHSANGVVNGDASSESTSHHTDSSGGGGSGSGGALEEARVRYKNSVL 120
           TSGLTIASPSGPSSHSANGVVNGDASSESTSHHTDSSGGGGSGSGGALEEARVRYKNSVL
Sbjct: 61  TSGLTIASPSGPSSHSANGVVNGDASSESTSHHTDSSGGGGSGSGGALEEARVRYKNSVL 120

Query: 121 ALRAILAAIPNSQKAKAYESGTSGHASPADQSEIDKLEQHLSNLRKELVVKNLHIKLLID 180
           ALRAILAAIPNSQKAKAYESGTSGHASPADQSEIDKLEQHLSNLRKELVVKNLHIKLLID
Sbjct: 121 ALRAILAAIPNSQKAKAYESGTSGHASPADQSEIDKLEQHLSNLRKELVVKNLHIKLLID 180

Query: 181 QLRDLVADVSTWQSPCSV 199
           QLRDLVADVSTWQSPCSV
Sbjct: 181 QLRDLVADVSTWQSPCSV 198

[Close]

BLAST of CSPI01G00490.1 vs. ExPASy TrEMBL
Match: A0A5A7USU3 (Mediator of RNA polymerase II transcription subunit 30 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold350G00490 PE=4 SV=1)

HSP 1 Score: 357.8 bits (917), Expect = 2.9e-95
Identity = 194/198 (97.98%), Postives = 195/198 (98.48%), Query Frame = 0


Query: 1   MEEKSTNVLALPQTTQELAMEGIKHLEETIESAFQILYSMNDELCNPTLWSTTSSTATTA 60
           MEEKST VLALPQTTQELAMEGIKHLEETIESAFQILYSMNDELCNPTLWSTTSSTATTA
Sbjct: 1   MEEKSTTVLALPQTTQELAMEGIKHLEETIESAFQILYSMNDELCNPTLWSTTSSTATTA 60

Query: 61  TSGLTIASPSGPSSHSANGVVNGDASSESTSHHTDSSGGGGSGSGGALEEARVRYKNSVL 120
           TSGLTIASP+GPSSHSANGVVNGDASSESTSHHTDSS GGGSGSGGALEEARVRYKNSVL
Sbjct: 61  TSGLTIASPNGPSSHSANGVVNGDASSESTSHHTDSSSGGGSGSGGALEEARVRYKNSVL 120

Query: 121 ALRAILAAIPNSQKAKAYESGTSGHASPADQSEIDKLEQHLSNLRKELVVKNLHIKLLID 180
           ALRAILAAIPNSQKAKAYESGTS HASPADQSEIDKLEQHLSNLRKELVVKNLHIKLLID
Sbjct: 121 ALRAILAAIPNSQKAKAYESGTSAHASPADQSEIDKLEQHLSNLRKELVVKNLHIKLLID 180

Query: 181 QLRDLVADVSTWQSPCSV 199
           QLRDLVADVSTWQSPCSV
Sbjct: 181 QLRDLVADVSTWQSPCSV 198

[Close]

BLAST of CSPI01G00490.1 vs. ExPASy TrEMBL
Match: A0A1S3CMZ7 (mediator of RNA polymerase II transcription subunit 30 OS=Cucumis melo OX=3656 GN=LOC103502315 PE=4 SV=1)

HSP 1 Score: 357.8 bits (917), Expect = 2.9e-95
Identity = 194/198 (97.98%), Postives = 195/198 (98.48%), Query Frame = 0


Query: 1   MEEKSTNVLALPQTTQELAMEGIKHLEETIESAFQILYSMNDELCNPTLWSTTSSTATTA 60
           MEEKST VLALPQTTQELAMEGIKHLEETIESAFQILYSMNDELCNPTLWSTTSSTATTA
Sbjct: 1   MEEKSTTVLALPQTTQELAMEGIKHLEETIESAFQILYSMNDELCNPTLWSTTSSTATTA 60

Query: 61  TSGLTIASPSGPSSHSANGVVNGDASSESTSHHTDSSGGGGSGSGGALEEARVRYKNSVL 120
           TSGLTIASP+GPSSHSANGVVNGDASSESTSHHTDSS GGGSGSGGALEEARVRYKNSVL
Sbjct: 61  TSGLTIASPNGPSSHSANGVVNGDASSESTSHHTDSSSGGGSGSGGALEEARVRYKNSVL 120

Query: 121 ALRAILAAIPNSQKAKAYESGTSGHASPADQSEIDKLEQHLSNLRKELVVKNLHIKLLID 180
           ALRAILAAIPNSQKAKAYESGTS HASPADQSEIDKLEQHLSNLRKELVVKNLHIKLLID
Sbjct: 121 ALRAILAAIPNSQKAKAYESGTSAHASPADQSEIDKLEQHLSNLRKELVVKNLHIKLLID 180

Query: 181 QLRDLVADVSTWQSPCSV 199
           QLRDLVADVSTWQSPCSV
Sbjct: 181 QLRDLVADVSTWQSPCSV 198

[Close]

BLAST of CSPI01G00490.1 vs. ExPASy TrEMBL
Match: A0A6J1JFZ2 (mediator of RNA polymerase II transcription subunit 30 OS=Cucurbita maxima OX=3661 GN=LOC111484777 PE=4 SV=1)

HSP 1 Score: 347.1 bits (889), Expect = 5.0e-92
Identity = 186/198 (93.94%), Postives = 193/198 (97.47%), Query Frame = 0


Query: 1   MEEKSTNVLALPQTTQELAMEGIKHLEETIESAFQILYSMNDELCNPTLWSTTSSTATTA 60
           MEEK TNVL+LPQTTQELAMEGIKHLEETIESAFQILYSMNDELCNPTLWSTTSSTATT 
Sbjct: 1   MEEKPTNVLSLPQTTQELAMEGIKHLEETIESAFQILYSMNDELCNPTLWSTTSSTATTT 60

Query: 61  TSGLTIASPSGPSSHSANGVVNGDASSESTSHHTDSSGGGGSGSGGALEEARVRYKNSVL 120
           TSGLTIASP+ PSSHS NGVVNGDASSEST+HHTD+S GGGSGSGGALEEARVRYKNSVL
Sbjct: 61  TSGLTIASPNAPSSHSTNGVVNGDASSESTNHHTDTSSGGGSGSGGALEEARVRYKNSVL 120

Query: 121 ALRAILAAIPNSQKAKAYESGTSGHASPADQSEIDKLEQHLSNLRKELVVKNLHIKLLID 180
           ALRAILAAIPNSQKAKA+ESGTSGHASPADQSEIDKLEQHLSNLRKEL+VKNLHIKLLID
Sbjct: 121 ALRAILAAIPNSQKAKAHESGTSGHASPADQSEIDKLEQHLSNLRKELMVKNLHIKLLID 180

Query: 181 QLRDLVADVSTWQSPCSV 199
           QLR+LVADVSTWQSPCSV
Sbjct: 181 QLRELVADVSTWQSPCSV 198

[Close]

BLAST of CSPI01G00490.1 vs. ExPASy TrEMBL
Match: A0A6J1E0F5 (mediator of RNA polymerase II transcription subunit 30 OS=Cucurbita moschata OX=3662 GN=LOC111429707 PE=4 SV=1)

HSP 1 Score: 347.1 bits (889), Expect = 5.0e-92
Identity = 186/198 (93.94%), Postives = 193/198 (97.47%), Query Frame = 0


Query: 1   MEEKSTNVLALPQTTQELAMEGIKHLEETIESAFQILYSMNDELCNPTLWSTTSSTATTA 60
           MEEK TNVL+LPQTTQELAMEGIKHLEETIESAFQILYSMNDELCNPTLWSTTSSTATT 
Sbjct: 1   MEEKPTNVLSLPQTTQELAMEGIKHLEETIESAFQILYSMNDELCNPTLWSTTSSTATTT 60

Query: 61  TSGLTIASPSGPSSHSANGVVNGDASSESTSHHTDSSGGGGSGSGGALEEARVRYKNSVL 120
           TSGLTIASP+ PSSHS NGVVNGDASSEST+HHTD+S GGGSGSGGALEEARVRYKNSVL
Sbjct: 61  TSGLTIASPNAPSSHSTNGVVNGDASSESTNHHTDTSSGGGSGSGGALEEARVRYKNSVL 120

Query: 121 ALRAILAAIPNSQKAKAYESGTSGHASPADQSEIDKLEQHLSNLRKELVVKNLHIKLLID 180
           ALRAILAAIPNSQKAKA+ESGTSGHASPADQSEIDKLEQHLSNLRKEL+VKNLHIKLLID
Sbjct: 121 ALRAILAAIPNSQKAKAHESGTSGHASPADQSEIDKLEQHLSNLRKELMVKNLHIKLLID 180

Query: 181 QLRDLVADVSTWQSPCSV 199
           QLR+LVADVSTWQSPCSV
Sbjct: 181 QLRELVADVSTWQSPCSV 198

[Close]

BLAST of CSPI01G00490.1 vs. NCBI nr
Match: XP_004138044.1 (mediator of RNA polymerase II transcription subunit 30 [Cucumis sativus] >KGN63478.1 hypothetical protein Csa_013518 [Cucumis sativus])

HSP 1 Score: 365.9 bits (938), Expect = 2.2e-97
Identity = 198/198 (100.00%), Postives = 198/198 (100.00%), Query Frame = 0


Query: 1   MEEKSTNVLALPQTTQELAMEGIKHLEETIESAFQILYSMNDELCNPTLWSTTSSTATTA 60
           MEEKSTNVLALPQTTQELAMEGIKHLEETIESAFQILYSMNDELCNPTLWSTTSSTATTA
Sbjct: 1   MEEKSTNVLALPQTTQELAMEGIKHLEETIESAFQILYSMNDELCNPTLWSTTSSTATTA 60

Query: 61  TSGLTIASPSGPSSHSANGVVNGDASSESTSHHTDSSGGGGSGSGGALEEARVRYKNSVL 120
           TSGLTIASPSGPSSHSANGVVNGDASSESTSHHTDSSGGGGSGSGGALEEARVRYKNSVL
Sbjct: 61  TSGLTIASPSGPSSHSANGVVNGDASSESTSHHTDSSGGGGSGSGGALEEARVRYKNSVL 120

Query: 121 ALRAILAAIPNSQKAKAYESGTSGHASPADQSEIDKLEQHLSNLRKELVVKNLHIKLLID 180
           ALRAILAAIPNSQKAKAYESGTSGHASPADQSEIDKLEQHLSNLRKELVVKNLHIKLLID
Sbjct: 121 ALRAILAAIPNSQKAKAYESGTSGHASPADQSEIDKLEQHLSNLRKELVVKNLHIKLLID 180

Query: 181 QLRDLVADVSTWQSPCSV 199
           QLRDLVADVSTWQSPCSV
Sbjct: 181 QLRDLVADVSTWQSPCSV 198

[Close]

BLAST of CSPI01G00490.1 vs. NCBI nr
Match: XP_008464424.1 (PREDICTED: mediator of RNA polymerase II transcription subunit 30 [Cucumis melo] >KAA0057767.1 mediator of RNA polymerase II transcription subunit 30 [Cucumis melo var. makuwa] >TYJ98448.1 mediator of RNA polymerase II transcription subunit 30 [Cucumis melo var. makuwa])

HSP 1 Score: 357.8 bits (917), Expect = 5.9e-95
Identity = 194/198 (97.98%), Postives = 195/198 (98.48%), Query Frame = 0


Query: 1   MEEKSTNVLALPQTTQELAMEGIKHLEETIESAFQILYSMNDELCNPTLWSTTSSTATTA 60
           MEEKST VLALPQTTQELAMEGIKHLEETIESAFQILYSMNDELCNPTLWSTTSSTATTA
Sbjct: 1   MEEKSTTVLALPQTTQELAMEGIKHLEETIESAFQILYSMNDELCNPTLWSTTSSTATTA 60

Query: 61  TSGLTIASPSGPSSHSANGVVNGDASSESTSHHTDSSGGGGSGSGGALEEARVRYKNSVL 120
           TSGLTIASP+GPSSHSANGVVNGDASSESTSHHTDSS GGGSGSGGALEEARVRYKNSVL
Sbjct: 61  TSGLTIASPNGPSSHSANGVVNGDASSESTSHHTDSSSGGGSGSGGALEEARVRYKNSVL 120

Query: 121 ALRAILAAIPNSQKAKAYESGTSGHASPADQSEIDKLEQHLSNLRKELVVKNLHIKLLID 180
           ALRAILAAIPNSQKAKAYESGTS HASPADQSEIDKLEQHLSNLRKELVVKNLHIKLLID
Sbjct: 121 ALRAILAAIPNSQKAKAYESGTSAHASPADQSEIDKLEQHLSNLRKELVVKNLHIKLLID 180

Query: 181 QLRDLVADVSTWQSPCSV 199
           QLRDLVADVSTWQSPCSV
Sbjct: 181 QLRDLVADVSTWQSPCSV 198

[Close]

BLAST of CSPI01G00490.1 vs. NCBI nr
Match: XP_022921424.1 (mediator of RNA polymerase II transcription subunit 30 [Cucurbita moschata] >XP_022987135.1 mediator of RNA polymerase II transcription subunit 30 [Cucurbita maxima] >XP_023515464.1 mediator of RNA polymerase II transcription subunit 30 [Cucurbita pepo subsp. pepo] >KAG6589379.1 Mediator of RNA polymerase II transcription subunit 30, partial [Cucurbita argyrosperma subsp. sororia] >KAG7023058.1 Mediator of RNA polymerase II transcription subunit 30 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 347.1 bits (889), Expect = 1.0e-91
Identity = 186/198 (93.94%), Postives = 193/198 (97.47%), Query Frame = 0


Query: 1   MEEKSTNVLALPQTTQELAMEGIKHLEETIESAFQILYSMNDELCNPTLWSTTSSTATTA 60
           MEEK TNVL+LPQTTQELAMEGIKHLEETIESAFQILYSMNDELCNPTLWSTTSSTATT 
Sbjct: 1   MEEKPTNVLSLPQTTQELAMEGIKHLEETIESAFQILYSMNDELCNPTLWSTTSSTATTT 60

Query: 61  TSGLTIASPSGPSSHSANGVVNGDASSESTSHHTDSSGGGGSGSGGALEEARVRYKNSVL 120
           TSGLTIASP+ PSSHS NGVVNGDASSEST+HHTD+S GGGSGSGGALEEARVRYKNSVL
Sbjct: 61  TSGLTIASPNAPSSHSTNGVVNGDASSESTNHHTDTSSGGGSGSGGALEEARVRYKNSVL 120

Query: 121 ALRAILAAIPNSQKAKAYESGTSGHASPADQSEIDKLEQHLSNLRKELVVKNLHIKLLID 180
           ALRAILAAIPNSQKAKA+ESGTSGHASPADQSEIDKLEQHLSNLRKEL+VKNLHIKLLID
Sbjct: 121 ALRAILAAIPNSQKAKAHESGTSGHASPADQSEIDKLEQHLSNLRKELMVKNLHIKLLID 180

Query: 181 QLRDLVADVSTWQSPCSV 199
           QLR+LVADVSTWQSPCSV
Sbjct: 181 QLRELVADVSTWQSPCSV 198

[Close]

BLAST of CSPI01G00490.1 vs. NCBI nr
Match: XP_038879540.1 (mediator of RNA polymerase II transcription subunit 30 [Benincasa hispida])

HSP 1 Score: 342.0 bits (876), Expect = 3.4e-90
Identity = 188/198 (94.95%), Postives = 190/198 (95.96%), Query Frame = 0


Query: 1   MEEKSTNVLALPQTTQELAMEGIKHLEETIESAFQILYSMNDELCNPTLWSTTSSTATTA 60
           MEEK TNVLALPQTTQELAMEGIKHLEETIESAFQILYSMNDELCNPTLWSTTSST TTA
Sbjct: 2   MEEKPTNVLALPQTTQELAMEGIKHLEETIESAFQILYSMNDELCNPTLWSTTSSTVTTA 61

Query: 61  TSGLTIASPSGPSSHSANGVVNGDASSESTSHHTDSSGGGGSGSGGALEEARVRYKNSVL 120
           TSGL IASP+GPSSHS NGVVNGDASSESTSHHTDSS GGGSGSGGALEEARVRYKNSVL
Sbjct: 62  TSGLAIASPNGPSSHSVNGVVNGDASSESTSHHTDSSSGGGSGSGGALEEARVRYKNSVL 121

Query: 121 ALRAILAAIPNSQKAKAYESGTSGHASPADQSEIDKLEQHLSNLRKELVVKNLHIKLLID 180
           ALRAILAAIPNSQKA AYESGTSG  SPADQSEIDKLEQHLS+LRKELVVKNLHIKLLID
Sbjct: 122 ALRAILAAIPNSQKATAYESGTSG--SPADQSEIDKLEQHLSSLRKELVVKNLHIKLLID 181

Query: 181 QLRDLVADVSTWQSPCSV 199
           QLRDLVADVSTWQSPCSV
Sbjct: 182 QLRDLVADVSTWQSPCSV 197

[Close]

BLAST of CSPI01G00490.1 vs. NCBI nr
Match: XP_022134927.1 (mediator of RNA polymerase II transcription subunit 30 [Momordica charantia])

HSP 1 Score: 322.0 bits (824), Expect = 3.6e-84
Identity = 173/198 (87.37%), Postives = 186/198 (93.94%), Query Frame = 0


Query: 1   MEEKSTNVLALPQTTQELAMEGIKHLEETIESAFQILYSMNDELCNPTLWSTTSSTATTA 60
           ME+K T++LALPQTTQELA+EGIKHLEETIESAFQILYSMNDELCNPTLWSTTSSTAT+A
Sbjct: 1   MEDKPTSILALPQTTQELAIEGIKHLEETIESAFQILYSMNDELCNPTLWSTTSSTATSA 60

Query: 61  TSGLTIASPSGPSSHSANGVVNGDASSESTSHHTDSSGGGGSGSGGALEEARVRYKNSVL 120
              L+IASP+GPS HS NGVVNGDASSEST+HH D+S GGG+GSGGALEEARVRY NSVL
Sbjct: 61  ---LSIASPNGPSPHSTNGVVNGDASSESTNHHADTSSGGGTGSGGALEEARVRYNNSVL 120

Query: 121 ALRAILAAIPNSQKAKAYESGTSGHASPADQSEIDKLEQHLSNLRKELVVKNLHIKLLID 180
           ALRAILAAIP+SQ AKAYESGTSGHASPADQSEIDKLEQHL NLRKELV KNLH+KLLID
Sbjct: 121 ALRAILAAIPSSQNAKAYESGTSGHASPADQSEIDKLEQHLCNLRKELVNKNLHLKLLID 180

Query: 181 QLRDLVADVSTWQSPCSV 199
           QLRDLVAD+STWQSPCSV
Sbjct: 181 QLRDLVADISTWQSPCSV 195

[Close]

BLAST of CSPI01G00490.1 vs. TAIR 10
Match: AT5G63480.1 (unknown protein; Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 183.0 bits (463), Expect = 2.4e-46
Identity = 105/188 (55.85%), Postives = 142/188 (75.53%), Query Frame = 0


Query: 14  TTQELAMEGIKHLEETIESAFQILYSMNDELCNPTLWSTTSSTATTATSGLTIASPSGPS 73
           TTQELAMEG K LEETIE+AFQI+ +MNDELCNP+LWST+++ ++ AT+          +
Sbjct: 13  TTQELAMEGEKQLEETIEAAFQIISAMNDELCNPSLWSTSATPSSAATT----------T 72

Query: 74  SHSANGVVNGDASS-ESTSHHTDSSGGGGSGSGG--ALEEARVRYKNSVLALRAILAAIP 133
             + + +V+ DA++ + TSHH++S+GGGG G  G   L+EA +RYKNSV +LRA+LAAIP
Sbjct: 73  GSNGSALVSADAAAIDGTSHHSESAGGGGGGGSGNSVLDEASLRYKNSVTSLRAVLAAIP 132

Query: 134 NSQKAKAYESGTSGHASPADQSEIDKLEQHLSNLRKELVVKNLHIKLLIDQLRDLVADVS 193
           NSQKAKA E   +G  SP  + EI+KLE+   +LR E+  KN+H+K LID+LR+L+AD+S
Sbjct: 133 NSQKAKASEM-QNGLGSPESEDEIEKLEEQALSLRMEIAKKNVHVKELIDKLRELIADIS 189

Query: 194 TWQSPCSV 199
           TWQSPCSV
Sbjct: 193 TWQSPCSV 189

The following BLAST results are available for this feature:

BLAST of CSPI01G00490.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FMV4	3.4e-45	55.85	Mediator of RNA polymerase II transcription subunit 30 OS=Arabidopsis thaliana O...	[more]

BLAST of CSPI01G00490.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LNW4	1.0e-97	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G001500 PE=4 SV=1	[more]
A0A5A7USU3	2.9e-95	97.98	Mediator of RNA polymerase II transcription subunit 30 OS=Cucumis melo var. maku...	[more]
A0A1S3CMZ7	2.9e-95	97.98	mediator of RNA polymerase II transcription subunit 30 OS=Cucumis melo OX=3656 G...	[more]
A0A6J1JFZ2	5.0e-92	93.94	mediator of RNA polymerase II transcription subunit 30 OS=Cucurbita maxima OX=36...	[more]
A0A6J1E0F5	5.0e-92	93.94	mediator of RNA polymerase II transcription subunit 30 OS=Cucurbita moschata OX=...	[more]

BLAST of CSPI01G00490.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004138044.1	2.2e-97	100.00	mediator of RNA polymerase II transcription subunit 30 [Cucumis sativus] >KGN634...	[more]
XP_008464424.1	5.9e-95	97.98	PREDICTED: mediator of RNA polymerase II transcription subunit 30 [Cucumis melo]...	[more]
XP_022921424.1	1.0e-91	93.94	mediator of RNA polymerase II transcription subunit 30 [Cucurbita moschata] >XP_...	[more]
XP_038879540.1	3.4e-90	94.95	mediator of RNA polymerase II transcription subunit 30 [Benincasa hispida]	[more]
XP_022134927.1	3.6e-84	87.37	mediator of RNA polymerase II transcription subunit 30 [Momordica charantia]	[more]

BLAST of CSPI01G00490.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G63480.1	2.4e-46	55.85	unknown protein; Has 30201 Blast hits to 17322 proteins in 780 species: Archae -...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 154..174
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 62..107
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 62..101
None	No IPR available	PANTHER	PTHR36406:SF2	MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 30	coord: 7..198
IPR034568	Mediator of RNA polymerase II transcription subunit 30	PANTHER	PTHR36406	MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 30	coord: 7..198

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CSPI01G00490	CSPI01G00490	gene

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI01G00490.1.utr3p1	CSPI01G00490.1.utr3p1	three_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI01G00490.1.cds3	CSPI01G00490.1.cds3	CDS
CSPI01G00490.1.cds2	CSPI01G00490.1.cds2	CDS
CSPI01G00490.1.cds1	CSPI01G00490.1.cds1	CDS

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI01G00490.1.utr5p1	CSPI01G00490.1.utr5p1	five_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CSPI01G00490.1	CSPI01G00490.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016592	mediator complex