Bhi07M000047 (mRNA) Wax gourd (B227) v1

Overview


Name	Bhi07M000047
Type	mRNA
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	calmodulin-like
Location	chr7: 2795086 .. 2795704 (+)
Sequence length	619
RNA-Seq Expression	Bhi07M000047
Synteny	Bhi07M000047

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTCTCCTAGAAACAAAGATAGCACGATTACAATAATGTGCATCCCACGTCTTCAAGTTTTGATGGCCAAGTATGAAGCAAATAAGTTGGTCAAGAAGCATGAAAAAAATGGTGAAGGTGTTAAACTAAAACGTCTCAATGCCATTGCTCGAGGTTCAACTCAAATCAACAATAATCAAGGAATGGAAACGAAAATATTAAAAGCTAACGACCATGTTGCGCTCAAGTTTAGTTCAAATGTTCATATAAAACCAAATAAGCAAATAGGAGGAAAAGCTCACGTACCTACCTCAAGAGTTCTTGATATAAAGGCGTCTAGAATGCAATTTAGTCGAGAGCAGATAAAGGAGATTTTTCAATACCATGATAGCGACAATGATGGTTTTCTCAACATAAAGGAGGTAACAAAAGCTTTTGGTTTCCTTGGCTCAATTCTTCCATTCTACAAAGCCTACCATGGGATGGTGTATGCTGATACTAATAATGATGGCCTTATCAGTGAGGATGAACTCGAAAAACTCATTGATTATGCGATTAAATTTATCAATAAGAAATGATTTTTTGAGCTACATAAACACGAATTTTATGTACTAAAGTAAATTTGCATTTTCAAGTCAAC

mRNA sequence

ATTCTCCTAGAAACAAAGATAGCACGATTACAATAATGTGCATCCCACGTCTTCAAGTTTTGATGGCCAAGTATGAAGCAAATAAGTTGGTCAAGAAGCATGAAAAAAATGGTGAAGGTGTTAAACTAAAACGTCTCAATGCCATTGCTCGAGGTTCAACTCAAATCAACAATAATCAAGGAATGGAAACGAAAATATTAAAAGCTAACGACCATGTTGCGCTCAAGTTTAGTTCAAATGTTCATATAAAACCAAATAAGCAAATAGGAGGAAAAGCTCACGTACCTACCTCAAGAGTTCTTGATATAAAGGCGTCTAGAATGCAATTTAGTCGAGAGCAGATAAAGGAGATTTTTCAATACCATGATAGCGACAATGATGGTTTTCTCAACATAAAGGAGGTAACAAAAGCTTTTGGTTTCCTTGGCTCAATTCTTCCATTCTACAAAGCCTACCATGGGATGGTGTATGCTGATACTAATAATGATGGCCTTATCAGTGAGGATGAACTCGAAAAACTCATTGATTATGCGATTAAATTTATCAATAAGAAATGATTTTTTGAGCTACATAAACACGAATTTTATGTACTAAAGTAAATTTGCATTTTCAAGTCAAC

Coding sequence (CDS)

TCTCCTAGAAACAAAGATAGCACGATTACAATAATGTGCATCCCACGTCTTCAAGTTTTGATGGCCAAGTATGAAGCAAATAAGTTGGTCAAGAAGCATGAAAAAAATGGTGAAGGTGTTAAACTAAAACGTCTCAATGCCATTGCTCGAGGTTCAACTCAAATCAACAATAATCAAGGAATGGAAACGAAAATATTAAAAGCTAACGACCATGTTGCGCTCAAGTTTAGTTCAAATGTTCATATAAAACCAAATAAGCAAATAGGAGGAAAAGCTCACGTACCTACCTCAAGAGTTCTTGATATAAAGGCGTCTAGAATGCAATTTAGTCGAGAGCAGATAAAGGAGATTTTTCAATACCATGATAGCGACAATGATGGTTTTCTCAACATAAAGGAGGTAACAAAAGCTTTTGGTTTCCTTGGCTCAATTCTTCCATTCTACAAAGCCTACCATGGGATGGTGTATGCTGATACTAATAATGATGGCCTTATCAGTGAGGATGAACTCGAAAAACTCATTGATTATGCGATTAAATTTATCAATAAGAAATGA

Protein sequence

SPRNKDSTITIMCIPRLQVLMAKYEANKLVKKHEKNGEGVKLKRLNAIARGSTQINNNQGMETKILKANDHVALKFSSNVHIKPNKQIGGKAHVPTSRVLDIKASRMQFSREQIKEIFQYHDSDNDGFLNIKEVTKAFGFLGSILPFYKAYHGMVYADTNNDGLISEDELEKLIDYAIKFINKK

Homology

[Close]

BLAST of Bhi07M000047 vs. TAIR 10
Match: AT3G50770.1 (calmodulin-like 41 )

HSP 1 Score: 42.0 bits (97), Expect = 6.2e-04
Identity = 32/120 (26.67%), Postives = 54/120 (45.00%), Query Frame = 0


Query: 56  NNNQGMETKILKANDHVALKFSSNVHIKPNKQIGGKAHVPTSRVLDIKASRM-QFSREQI 115
           N+ +   TK LK N    L F  N    P        + P S   D    +  Q S+E++
Sbjct: 11  NSFKWFSTKTLKLN----LSF-QNRRRSPKSNSSSTLNSPRSNSDDNNNIKSHQASKEEL 70

Query: 116 KEIFQYHDSDNDGFLNIKEVTKAFGFLGSILPFYKAYHGMVYADTNNDGLISEDELEKLI 175
           +++F + DSD DG ++  E+   FG +G  +    A   +   DT+ DG +  ++   L+
Sbjct: 71  RQVFSHFDSDGDGKISAFELRHYFGSVGEYISHEAAQEAINEVDTDADGSLGFEDFVGLM 125

[Close]

BLAST of Bhi07M000047 vs. ExPASy TrEMBL
Match: A0A0A0KLF3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G067670 PE=4 SV=1)

HSP 1 Score: 236.1 bits (601), Expect = 1.2e-58
Identity = 123/173 (71.10%), Postives = 136/173 (78.61%), Query Frame = 0


Query: 12  MCIPRLQVLMAKYEANKLVKKHEKNGEGVKLKRLNAIARGSTQINNNQGMETKILKANDH 71
           M IP+LQ+  AKYEA+KL+KK+E+NGEGV L   N  ARGSTQIN N+ M TK LK ND 
Sbjct: 28  MHIPQLQMSKAKYEASKLMKKYEQNGEGVILSAFNDNARGSTQINTNREMATKKLKPNDD 87

Query: 72  VALKFSSNVHIKPNKQIGGKAHVPTSRVLDIKASRMQFSREQIKEIFQYHDSDNDGFLNI 131
           VA K SSN  +K N QI GKA VP  RV D KAS MQFSREQIKEIFQY+DSD DGFLNI
Sbjct: 88  VAHKLSSNAQLKSNNQIRGKAQVPIKRVYDTKASNMQFSREQIKEIFQYYDSDRDGFLNI 147

Query: 132 KEVTKAFGFLGSILPFYKAYHGMVYADTNNDGLISEDELEKLIDYAIKFINKK 185
           +EVTKAF  LGSI PF KAYHGMVYADTN DGLI EDEL+KLI+YA KF+ KK
Sbjct: 148 REVTKAFALLGSIFPFNKAYHGMVYADTNKDGLIGEDELDKLINYANKFMKKK 200

[Close]

BLAST of Bhi07M000047 vs. ExPASy TrEMBL
Match: A0A0A0KIY4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G068170 PE=4 SV=1)

HSP 1 Score: 230.3 bits (586), Expect = 6.4e-57
Identity = 122/176 (69.32%), Postives = 140/176 (79.55%), Query Frame = 0


Query: 12  MCIPRLQVLMAKYEANKLVKKHEKNGEGVKLKRLNAIARGSTQINNNQGMETK-ILKAND 71
           M I  LQV  AKYEA+KL+KKHE N EGV LK  N I   ST+INN Q M T+ ILK ++
Sbjct: 1   MYISNLQVSKAKYEASKLMKKHENNDEGVPLKAFNGIPPASTKINNKQAMATEIILKPSN 60

Query: 72  H-VALKFSSNVHIKPNKQIG-GKAHVPTSRVLDIKASRMQFSREQIKEIFQYHDSDNDGF 131
           H VA   +SN ++ P+KQ+G GKA V T+ VLDIKAS MQFSREQIKEIFQYHDSDNDGF
Sbjct: 61  HPVAHYLTSNAYMIPSKQLGAGKAQVTTTSVLDIKASNMQFSREQIKEIFQYHDSDNDGF 120

Query: 132 LNIKEVTKAFGFLGSILPFYKAYHGMVYADTNNDGLISEDELEKLIDYAIKFINKK 185
           LN++EVTKAF FLG+ILP YKAYHGMVYAD N DGLIS+DELEKL+DYA KF NK+
Sbjct: 121 LNLREVTKAFAFLGTILPLYKAYHGMVYADANKDGLISKDELEKLVDYAYKFFNKR 176

[Close]

BLAST of Bhi07M000047 vs. ExPASy TrEMBL
Match: A0A5A7VI66 (Putative calcium-binding protein CML11 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold17G001550 PE=4 SV=1)

HSP 1 Score: 196.4 bits (498), Expect = 1.0e-46
Identity = 104/173 (60.12%), Postives = 127/173 (73.41%), Query Frame = 0


Query: 12  MCIPRLQVLMAKYEANKLVKKHEKNGEGVKLKRLNAIARGSTQINNNQGMETKILKANDH 71
           M I +LQ  MA YEA   +K +EK+G+ V++K  N + R STQI   QG+  KILK++D 
Sbjct: 1   MYISKLQASMAMYEARMFIKHYEKDGDAVQVKGFNDV-RSSTQI-GYQGIVPKILKSSDD 60

Query: 72  VALKFSSNVHIKPNKQIGGKAHVPTSRVLDIKASRMQFSREQIKEIFQYHDSDNDGFLNI 131
           VA K S   HI+P +Q GGK     +R LDIK+S M FSR+QIKEIF+YHD++NDGFLNI
Sbjct: 61  VAHKLSLEAHIQPTQQTGGKGKKSIARALDIKSSNMSFSRQQIKEIFRYHDNNNDGFLNI 120

Query: 132 KEVTKAFGFLGSILPFYKAYHGMVYADTNNDGLISEDELEKLIDYAIKFINKK 185
            E+TKAF FLGS+ PFYKA +GMVYAD N DGLISE EL+KLIDYA K I KK
Sbjct: 121 VELTKAFAFLGSMFPFYKACYGMVYADANADGLISEAELDKLIDYATKIIKKK 171

[Close]

BLAST of Bhi07M000047 vs. ExPASy TrEMBL
Match: A0A0A0KMQ1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G067410 PE=4 SV=1)

HSP 1 Score: 194.5 bits (493), Expect = 3.9e-46
Identity = 103/173 (59.54%), Postives = 127/173 (73.41%), Query Frame = 0


Query: 12  MCIPRLQVLMAKYEANKLVKKHEKNGEGVKLKRLNAIARGSTQINNNQGMETKILKANDH 71
           M I +LQ  MA YEA+ L+K++EKNG+ VK+K  + + R STQI N + +  KILK +D 
Sbjct: 1   MYISKLQASMAMYEASMLIKQYEKNGDAVKVKAFDDV-RSSTQIGNRESV-LKILKPSDG 60

Query: 72  VALKFSSNVHIKPNKQIGGKAHVPTSRVLDIKASRMQFSREQIKEIFQYHDSDNDGFLNI 131
           VA K  S  HI+P ++ GGK     +RVLD K+S M  SRE+IKEIFQYHD++NDGFLN 
Sbjct: 61  VAHKLRSEAHIQPTQRTGGKGKKSITRVLDRKSSNMSLSRERIKEIFQYHDNNNDGFLNR 120

Query: 132 KEVTKAFGFLGSILPFYKAYHGMVYADTNNDGLISEDELEKLIDYAIKFINKK 185
            E+TKAF FLGS+ PFYKA +GMVYAD N DGLISE EL+KLIDYA K I KK
Sbjct: 121 MELTKAFAFLGSMFPFYKACYGMVYADANEDGLISEAELDKLIDYASKIIKKK 171

[Close]

BLAST of Bhi07M000047 vs. ExPASy TrEMBL
Match: A0A6J1EP72 (calmodulin-2/4-like OS=Cucurbita moschata OX=3662 GN=LOC111436455 PE=4 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 1.9e-45
Identity = 102/173 (58.96%), Postives = 123/173 (71.10%), Query Frame = 0


Query: 12  MCIPRLQVLMAKYEANKLVKKHEKNGEGVKLKRLNAIARGSTQINNNQGMETKILKANDH 71
           M IP+LQ  MAKYEA++++KK  +NG+ V+LK  N IAR STQ+ NNQ M  +IL+ N +
Sbjct: 1   MFIPKLQASMAKYEASEMIKKSTRNGDNVQLKAFNGIARASTQV-NNQSMVPEILRDNKN 60

Query: 72  VALKFSSNVHIKPNKQIGGKAHVPTSRVLDIKASRMQFSREQIKEIFQYHDSDNDGFLNI 131
           V  KFSSN H                    +KAS MQFSREQ+KEIF+YHDSD DGFLNI
Sbjct: 61  VGPKFSSNTH--------------------LKASNMQFSREQMKEIFRYHDSDKDGFLNI 120

Query: 132 KEVTKAFGFLGSILPFYKAYHGMVYADTNNDGLISEDELEKLIDYAIKFINKK 185
           +E+TKAF FLGSI+PF KA +GM YAD N DGLISE EL+KL+DYA KFI KK
Sbjct: 121 RELTKAFAFLGSIIPFEKASYGMTYADANKDGLISEAELDKLVDYAQKFIKKK 152

The following BLAST results are available for this feature:

BLAST of Bhi07M000047 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G50770.1	6.2e-04	26.67	calmodulin-like 41	[more]

BLAST of Bhi07M000047 vs. TAIR 10


Match Name	E-value	Identity	Description

BLAST of Bhi07M000047 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KLF3	1.2e-58	71.10	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G067670 PE=4 SV=1	[more]
A0A0A0KIY4	6.4e-57	69.32	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G068170 PE=4 SV=1	[more]
A0A5A7VI66	1.0e-46	60.12	Putative calcium-binding protein CML11 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A0A0KMQ1	3.9e-46	59.54	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G067410 PE=4 SV=1	[more]
A0A6J1EP72	1.9e-45	58.96	calmodulin-2/4-like OS=Cucurbita moschata OX=3662 GN=LOC111436455 PE=4 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 111..173 e-value: 4.2E-8 score: 33.5
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 109..144 score: 12.449146
None	No IPR available	GENE3D	1.10.238.10		coord: 22..177 e-value: 1.1E-11 score: 46.9
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 122..134
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 158..170
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 105..173

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Bhi07G000047	Bhi07G000047	gene

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Bhi07M000047.utr5p1	Bhi07M000047.utr5p1	five_prime_UTR

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Bhi07M000047.exon1	Bhi07M000047.exon1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
cds.Bhi07M000047	cds.Bhi07M000047	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Bhi07M000047.utr3p1	Bhi07M000047.utr3p1	three_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Bhi07M000047	Bhi07M000047-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding