Bhi04M000769 (mRNA) Wax gourd (B227) v1

Overview


Name	Bhi04M000769
Type	mRNA
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	Elongation factor 1-gamma
Location	chr4: 23749034 .. 23749332 (-)
Sequence length	299
RNA-Seq Expression	Bhi04M000769
Synteny	Bhi04M000769

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GATCTTGGGACATGTACGATCCAGAGGGATATTCTCTTTGGTTCTGTGATTACAAGTACAATGATGAGAATACTGTTTCATTCGTGACTTTGAACAAGGTTGGTGGCTTTCTCCAACGAATGGATCTCGCTCGTAAATATGCTTTCAGGAAGATGTTAGTGATTGGATCAGAACCTCCATTTAAGGTGAAAGGTTTATGGCTTTTCCGTGGACAAGAGATCCCAAAATTCGTCCTGGACGAGTGCTACGACATTGAACTTTATGAATGGAGGAAGGTTGATATATCGAACAAGGGATAA

mRNA sequence

GATCTTGGGACATGTACGATCCAGAGGGATATTCTCTTTGGTTCTGTGATTACAAGTACAATGATGAGAATACTGTTTCATTCGTGACTTTGAACAAGGTTGGTGGCTTTCTCCAACGAATGGATCTCGCTCGTAAATATGCTTTCAGGAAGATGTTAGTGATTGGATCAGAACCTCCATTTAAGGTGAAAGGTTTATGGCTTTTCCGTGGACAAGAGATCCCAAAATTCGTCCTGGACGAGTGCTACGACATTGAACTTTATGAATGGAGGAAGGTTGATATATCGAACAAGGGATAA

Coding sequence (CDS)

GATCTTGGGACATGTACGATCCAGAGGGATATTCTCTTTGGTTCTGTGATTACAAGTACAATGATGAGAATACTGTTTCATTCGTGACTTTGAACAAGGTTGGTGGCTTTCTCCAACGAATGGATCTCGCTCGTAAATATGCTTTCAGGAAGATGTTAGTGATTGGATCAGAACCTCCATTTAAGGTGAAAGGTTTATGGCTTTTCCGTGGACAAGAGATCCCAAAATTCGTCCTGGACGAGTGCTACGACATTGAACTTTATGAATGGAGGAAGGTTGATATATCGAACAAGGGATAA

Protein sequence

SWDMYDPEGYSLWFCDYKYNDENTVSFVTLNKVGGFLQRMDLARKYAFRKMLVIGSEPPFKVKGLWLFRGQEIPKFVLDECYDIELYEWRKVDISNKG

Homology

[Close]

BLAST of Bhi04M000769 vs. TAIR 10
Match: AT1G09640.1 (Translation elongation factor EF1B, gamma chain )

HSP 1 Score: 186.4 bits (472), Expect = 1.1e-47
Identity = 82/96 (85.42%), Postives = 89/96 (92.71%), Query Frame = 0


Query: 2   WDMYDPEGYSLWFCDYKYNDENTVSFVTLNKVGGFLQRMDLARKYAFRKMLVIGSEPPFK 61
           WDMYDPEGYSLWFCDYKYNDEN VSFVTLNKVGGFLQRMDLARKY+F KML+ GSE PFK
Sbjct: 291 WDMYDPEGYSLWFCDYKYNDENMVSFVTLNKVGGFLQRMDLARKYSFGKMLICGSEGPFK 350

Query: 62  VKGLWLFRGQEIPKFVLDECYDIELYEWRKVDISNK 98
           VKGLWLFRG EIPKF++DE YD+ELYEW KVDIS++
Sbjct: 351 VKGLWLFRGPEIPKFIMDEVYDMELYEWTKVDISDE 386

[Close]

BLAST of Bhi04M000769 vs. TAIR 10
Match: AT1G57720.1 (Translation elongation factor EF1B, gamma chain )

HSP 1 Score: 186.4 bits (472), Expect = 1.1e-47
Identity = 82/96 (85.42%), Postives = 89/96 (92.71%), Query Frame = 0


Query: 2   WDMYDPEGYSLWFCDYKYNDENTVSFVTLNKVGGFLQRMDLARKYAFRKMLVIGSEPPFK 61
           WDMYDPEGYSLWFCDYKYNDEN VSFVTLNKVGGFLQRMDLARKY+F KML+ GSE PFK
Sbjct: 290 WDMYDPEGYSLWFCDYKYNDENMVSFVTLNKVGGFLQRMDLARKYSFGKMLICGSEGPFK 349

Query: 62  VKGLWLFRGQEIPKFVLDECYDIELYEWRKVDISNK 98
           VKGLWLFRG EIPKF++DE YD+ELYEW KVDIS++
Sbjct: 350 VKGLWLFRGPEIPKFIMDEVYDMELYEWTKVDISDE 385

[Close]

BLAST of Bhi04M000769 vs. TAIR 10
Match: AT1G57720.2 (Translation elongation factor EF1B, gamma chain )

HSP 1 Score: 186.4 bits (472), Expect = 1.1e-47
Identity = 82/96 (85.42%), Postives = 89/96 (92.71%), Query Frame = 0


Query: 2   WDMYDPEGYSLWFCDYKYNDENTVSFVTLNKVGGFLQRMDLARKYAFRKMLVIGSEPPFK 61
           WDMYDPEGYSLWFCDYKYNDEN VSFVTLNKVGGFLQRMDLARKY+F KML+ GSE PFK
Sbjct: 290 WDMYDPEGYSLWFCDYKYNDENMVSFVTLNKVGGFLQRMDLARKYSFGKMLICGSEGPFK 349

Query: 62  VKGLWLFRGQEIPKFVLDECYDIELYEWRKVDISNK 98
           VKGLWLFRG EIPKF++DE YD+ELYEW KVDIS++
Sbjct: 350 VKGLWLFRGPEIPKFIMDEVYDMELYEWTKVDISDE 385

[Close]

BLAST of Bhi04M000769 vs. TAIR 10
Match: AT1G09640.2 (Translation elongation factor EF1B, gamma chain )

HSP 1 Score: 72.8 bits (177), Expect = 1.7e-13
Identity = 32/50 (64.00%), Postives = 40/50 (80.00%), Query Frame = 0


Query: 49  RKMLVIGSEPPF-KVKGLWLFRGQEIPKFVLDECYDIELYEWRKVDISNK 98
           R    + ++P F KVKGLWLFRG EIPKF++DE YD+ELYEW KVDIS++
Sbjct: 188 RYFWTVVNQPNFTKVKGLWLFRGPEIPKFIMDEVYDMELYEWTKVDISDE 237

[Close]

BLAST of Bhi04M000769 vs. ExPASy Swiss-Prot
Match: Q9FUM1 (Elongation factor 1-gamma OS=Prunus avium OX=42229 PE=2 SV=1)

HSP 1 Score: 194.1 bits (492), Expect = 7.3e-49
Identity = 85/96 (88.54%), Postives = 92/96 (95.83%), Query Frame = 0


Query: 2   WDMYDPEGYSLWFCDYKYNDENTVSFVTLNKVGGFLQRMDLARKYAFRKMLVIGSEPPFK 61
           WDMYDPEGYSLWFC+YKYNDENTVSFVTLNKVGGFLQRMDLARKYAF KMLVIGSEPPFK
Sbjct: 299 WDMYDPEGYSLWFCEYKYNDENTVSFVTLNKVGGFLQRMDLARKYAFGKMLVIGSEPPFK 358

Query: 62  VKGLWLFRGQEIPKFVLDECYDIELYEWRKVDISNK 98
           VKGLWLFRGQEIP FV++ECYD+ELY W KVD+S++
Sbjct: 359 VKGLWLFRGQEIPPFVMEECYDMELYNWTKVDLSDE 394

[Close]

BLAST of Bhi04M000769 vs. ExPASy Swiss-Prot
Match: Q9ZRI7 (Elongation factor 1-gamma 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g0220600 PE=2 SV=1)

HSP 1 Score: 193.7 bits (491), Expect = 9.5e-49
Identity = 86/96 (89.58%), Postives = 91/96 (94.79%), Query Frame = 0


Query: 2   WDMYDPEGYSLWFCDYKYNDENTVSFVTLNKVGGFLQRMDLARKYAFRKMLVIGSEPPFK 61
           WDMYDPEGYSLWFCDYKYNDENTVSFVT+NKVGGFLQRMDL RKYAF KMLVIGSEPPFK
Sbjct: 295 WDMYDPEGYSLWFCDYKYNDENTVSFVTMNKVGGFLQRMDLCRKYAFGKMLVIGSEPPFK 354

Query: 62  VKGLWLFRGQEIPKFVLDECYDIELYEWRKVDISNK 98
           VKGLWLFRG EIPKFV+DE YD+ELYEW KVDIS++
Sbjct: 355 VKGLWLFRGPEIPKFVMDEVYDMELYEWTKVDISDE 390

[Close]

BLAST of Bhi04M000769 vs. ExPASy Swiss-Prot
Match: Q6YW46 (Elongation factor 1-gamma 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g0220500 PE=2 SV=2)

HSP 1 Score: 193.7 bits (491), Expect = 9.5e-49
Identity = 86/96 (89.58%), Postives = 91/96 (94.79%), Query Frame = 0


Query: 2   WDMYDPEGYSLWFCDYKYNDENTVSFVTLNKVGGFLQRMDLARKYAFRKMLVIGSEPPFK 61
           WDMYDPEGYSLWFCDYKYNDENTVSFVT+NKVGGFLQRMDL RKYAF KMLVIGSEPPFK
Sbjct: 295 WDMYDPEGYSLWFCDYKYNDENTVSFVTMNKVGGFLQRMDLCRKYAFGKMLVIGSEPPFK 354

Query: 62  VKGLWLFRGQEIPKFVLDECYDIELYEWRKVDISNK 98
           VKGLWLFRG EIPKFV+DE YD+ELYEW KVDIS++
Sbjct: 355 VKGLWLFRGPEIPKFVMDEVYDMELYEWTKVDISDE 390

[Close]

BLAST of Bhi04M000769 vs. ExPASy Swiss-Prot
Match: Q5Z627 (Elongation factor 1-gamma 3 OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g0571400 PE=2 SV=1)

HSP 1 Score: 191.8 bits (486), Expect = 3.6e-48
Identity = 84/96 (87.50%), Postives = 91/96 (94.79%), Query Frame = 0


Query: 2   WDMYDPEGYSLWFCDYKYNDENTVSFVTLNKVGGFLQRMDLARKYAFRKMLVIGSEPPFK 61
           WDMYDPEGYSLWFCDYKYNDENTVSFVT+NKVGGFLQRMDL RKYAF KMLVIGS PPFK
Sbjct: 293 WDMYDPEGYSLWFCDYKYNDENTVSFVTMNKVGGFLQRMDLCRKYAFGKMLVIGSTPPFK 352

Query: 62  VKGLWLFRGQEIPKFVLDECYDIELYEWRKVDISNK 98
           VKGLWLFRGQ+IPKFV+DE YD+ELYEW KVD+S++
Sbjct: 353 VKGLWLFRGQDIPKFVMDEVYDMELYEWTKVDLSDE 388

[Close]

BLAST of Bhi04M000769 vs. ExPASy Swiss-Prot
Match: O04487 (Probable elongation factor 1-gamma 1 OS=Arabidopsis thaliana OX=3702 GN=At1g09640 PE=1 SV=1)

HSP 1 Score: 186.4 bits (472), Expect = 1.5e-46
Identity = 82/96 (85.42%), Postives = 89/96 (92.71%), Query Frame = 0


Query: 2   WDMYDPEGYSLWFCDYKYNDENTVSFVTLNKVGGFLQRMDLARKYAFRKMLVIGSEPPFK 61
           WDMYDPEGYSLWFCDYKYNDEN VSFVTLNKVGGFLQRMDLARKY+F KML+ GSE PFK
Sbjct: 291 WDMYDPEGYSLWFCDYKYNDENMVSFVTLNKVGGFLQRMDLARKYSFGKMLICGSEGPFK 350

Query: 62  VKGLWLFRGQEIPKFVLDECYDIELYEWRKVDISNK 98
           VKGLWLFRG EIPKF++DE YD+ELYEW KVDIS++
Sbjct: 351 VKGLWLFRGPEIPKFIMDEVYDMELYEWTKVDISDE 386

[Close]

BLAST of Bhi04M000769 vs. ExPASy TrEMBL
Match: A0A5D3DRU3 (Elongation factor 1-gamma-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold14G00810 PE=4 SV=1)

HSP 1 Score: 202.6 bits (514), Expect = 7.6e-49
Identity = 90/96 (93.75%), Postives = 94/96 (97.92%), Query Frame = 0


Query: 2   WDMYDPEGYSLWFCDYKYNDENTVSFVTLNKVGGFLQRMDLARKYAFRKMLVIGSEPPFK 61
           WDMYDPEGYSLWFCDYKYNDEN VSFVTLNKVGGFLQRMDLARKYAF KMLVIGSEPPFK
Sbjct: 384 WDMYDPEGYSLWFCDYKYNDENIVSFVTLNKVGGFLQRMDLARKYAFGKMLVIGSEPPFK 443

Query: 62  VKGLWLFRGQEIPKFVLDECYDIELYEWRKVDISNK 98
           VKGLWLFRGQEIPKF+LDECYD+ELYEWRKVDIS++
Sbjct: 444 VKGLWLFRGQEIPKFILDECYDMELYEWRKVDISDE 479

[Close]

BLAST of Bhi04M000769 vs. ExPASy TrEMBL
Match: A0A1S3C550 (elongation factor 1-gamma-like OS=Cucumis melo OX=3656 GN=LOC103496769 PE=4 SV=1)

HSP 1 Score: 202.6 bits (514), Expect = 7.6e-49
Identity = 90/96 (93.75%), Postives = 94/96 (97.92%), Query Frame = 0


Query: 2   WDMYDPEGYSLWFCDYKYNDENTVSFVTLNKVGGFLQRMDLARKYAFRKMLVIGSEPPFK 61
           WDMYDPEGYSLWFCDYKYNDEN VSFVTLNKVGGFLQRMDLARKYAF KMLVIGSEPPFK
Sbjct: 299 WDMYDPEGYSLWFCDYKYNDENIVSFVTLNKVGGFLQRMDLARKYAFGKMLVIGSEPPFK 358

Query: 62  VKGLWLFRGQEIPKFVLDECYDIELYEWRKVDISNK 98
           VKGLWLFRGQEIPKF+LDECYD+ELYEWRKVDIS++
Sbjct: 359 VKGLWLFRGQEIPKFILDECYDMELYEWRKVDISDE 394

[Close]

BLAST of Bhi04M000769 vs. ExPASy TrEMBL
Match: A0A072USZ0 (Putative gamma class glutathione transferase EF1Bgamma2 OS=Medicago truncatula OX=3880 GN=25494379 PE=2 SV=1)

HSP 1 Score: 202.2 bits (513), Expect = 9.9e-49
Identity = 90/96 (93.75%), Postives = 94/96 (97.92%), Query Frame = 0


Query: 2   WDMYDPEGYSLWFCDYKYNDENTVSFVTLNKVGGFLQRMDLARKYAFRKMLVIGSEPPFK 61
           WDMYDPEGYSLWFCDYKYNDENTVSFVTLNKVGGFLQRMDLARKYAF KMLVIGSEPPFK
Sbjct: 296 WDMYDPEGYSLWFCDYKYNDENTVSFVTLNKVGGFLQRMDLARKYAFGKMLVIGSEPPFK 355

Query: 62  VKGLWLFRGQEIPKFVLDECYDIELYEWRKVDISNK 98
           VKGLWLFRGQEIPKFV+DECYD+ELYEW KVDIS++
Sbjct: 356 VKGLWLFRGQEIPKFVMDECYDMELYEWTKVDISDE 391

[Close]

BLAST of Bhi04M000769 vs. ExPASy TrEMBL
Match: A0A072UTU2 (Translation elongation factor EF1B, gamma chain OS=Medicago truncatula OX=3880 GN=25494379 PE=3 SV=1)

HSP 1 Score: 202.2 bits (513), Expect = 9.9e-49
Identity = 90/96 (93.75%), Postives = 94/96 (97.92%), Query Frame = 0


Query: 2   WDMYDPEGYSLWFCDYKYNDENTVSFVTLNKVGGFLQRMDLARKYAFRKMLVIGSEPPFK 61
           WDMYDPEGYSLWFCDYKYNDENTVSFVTLNKVGGFLQRMDLARKYAF KMLVIGSEPPFK
Sbjct: 261 WDMYDPEGYSLWFCDYKYNDENTVSFVTLNKVGGFLQRMDLARKYAFGKMLVIGSEPPFK 320

Query: 62  VKGLWLFRGQEIPKFVLDECYDIELYEWRKVDISNK 98
           VKGLWLFRGQEIPKFV+DECYD+ELYEW KVDIS++
Sbjct: 321 VKGLWLFRGQEIPKFVMDECYDMELYEWTKVDISDE 356

[Close]

BLAST of Bhi04M000769 vs. ExPASy TrEMBL
Match: A0A2Z6MV63 (Uncharacterized protein OS=Trifolium subterraneum OX=3900 GN=TSUD_282160 PE=4 SV=1)

HSP 1 Score: 202.2 bits (513), Expect = 9.9e-49
Identity = 90/96 (93.75%), Postives = 94/96 (97.92%), Query Frame = 0


Query: 2   WDMYDPEGYSLWFCDYKYNDENTVSFVTLNKVGGFLQRMDLARKYAFRKMLVIGSEPPFK 61
           WDMYDPEGYSLWFCDYKYNDENTVSFVTLNKVGGFLQRMDLARKYAF KMLVIGSEPPFK
Sbjct: 295 WDMYDPEGYSLWFCDYKYNDENTVSFVTLNKVGGFLQRMDLARKYAFGKMLVIGSEPPFK 354

Query: 62  VKGLWLFRGQEIPKFVLDECYDIELYEWRKVDISNK 98
           VKGLWLFRGQEIPKFV+DECYD+ELYEW KVDIS++
Sbjct: 355 VKGLWLFRGQEIPKFVMDECYDMELYEWTKVDISDE 390

The following BLAST results are available for this feature:

BLAST of Bhi04M000769 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G09640.1	1.1e-47	85.42	Translation elongation factor EF1B, gamma chain	[more]
AT1G57720.1	1.1e-47	85.42	Translation elongation factor EF1B, gamma chain	[more]
AT1G57720.2	1.1e-47	85.42	Translation elongation factor EF1B, gamma chain	[more]
AT1G09640.2	1.7e-13	64.00	Translation elongation factor EF1B, gamma chain	[more]

BLAST of Bhi04M000769 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FUM1	7.3e-49	88.54	Elongation factor 1-gamma OS=Prunus avium OX=42229 PE=2 SV=1	[more]
Q9ZRI7	9.5e-49	89.58	Elongation factor 1-gamma 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g022...	[more]
Q6YW46	9.5e-49	89.58	Elongation factor 1-gamma 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g022...	[more]
Q5Z627	3.6e-48	87.50	Elongation factor 1-gamma 3 OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g057...	[more]
O04487	1.5e-46	85.42	Probable elongation factor 1-gamma 1 OS=Arabidopsis thaliana OX=3702 GN=At1g0964...	[more]

BLAST of Bhi04M000769 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3DRU3	7.6e-49	93.75	Elongation factor 1-gamma-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s...	[more]
A0A1S3C550	7.6e-49	93.75	elongation factor 1-gamma-like OS=Cucumis melo OX=3656 GN=LOC103496769 PE=4 SV=1	[more]
A0A072USZ0	9.9e-49	93.75	Putative gamma class glutathione transferase EF1Bgamma2 OS=Medicago truncatula O...	[more]
A0A072UTU2	9.9e-49	93.75	Translation elongation factor EF1B, gamma chain OS=Medicago truncatula OX=3880 G...	[more]
A0A2Z6MV63	9.9e-49	93.75	Uncharacterized protein OS=Trifolium subterraneum OX=3900 GN=TSUD_282160 PE=4 SV...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001662	Elongation factor 1B gamma, C-terminal	SMART	SM01183	EF1G_2	coord: 1..72 e-value: 5.6E-23 score: 92.3
IPR001662	Elongation factor 1B gamma, C-terminal	PFAM	PF00647	EF1G	coord: 2..72 e-value: 2.7E-26 score: 91.6
IPR001662	Elongation factor 1B gamma, C-terminal	PROSITE	PS50040	EF1G_C	coord: 1..98 score: 48.195202
IPR036433	Elongation factor EF1B gamma, C-terminal domain superfamily	GENE3D	3.30.70.1010	Translation elongation factor EF1B, gamma chain, conserved domain	coord: 1..98 e-value: 2.4E-42 score: 146.3
IPR036433	Elongation factor EF1B gamma, C-terminal domain superfamily	SUPERFAMILY	89942	eEF1-gamma domain	coord: 2..96
IPR044628	Elongation factor 1-gamma, plant	PANTHER	PTHR44372	ELONGATION FACTOR 1-GAMMA 1-RELATED	coord: 2..96
None	No IPR available	PANTHER	PTHR44372:SF5	SUBFAMILY NOT NAMED	coord: 2..96

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Bhi04G000769	Bhi04G000769	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Bhi04M000769.exon1	Bhi04M000769.exon1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
cds.Bhi04M000769	cds.Bhi04M000769	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Bhi04M000769	Bhi04M000769-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006414	translational elongation
biological_process	GO:0006749	glutathione metabolic process
molecular_function	GO:0004364	glutathione transferase activity
molecular_function	GO:0003746	translation elongation factor activity