Tan0020796 (gene) Snake gourd v1

Overview
NameTan0020796
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
Descriptionpolygalacturonase inhibitor-like
LocationLG03: 48281235 .. 48282637 (+)
RNA-Seq ExpressionTan0020796
SyntenyTan0020796
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CACACATTCAAACACATCTTTAAAAATCGAAACCAAATCTCCAAAACTTACTCACTGAAGTCCATGGCTTCCAATCTCAAACTCCCATGGCTGCCCCTCTTTCTCTTGGTCTTCTTCATCTTCATCACCATTTCTTTTGCAGAACTCTGCAACCCAAATGACAAAAAAGCCCTCCTCAATATCAAAAAAGCCTTCAACAATCCTTACATCCTCACCTCCTGGGACCCTGAAACCGACTGCTGCCACTGGTACTGTGTCAAGTGCAATCGAACCACTCACCGGATCACTTCCCTTACCATCTTCGCCGACGACCGACTCTCCGGCCAAATCCCACCGGAAGTCGGCGACCTTCCATTTTTAGAAACTCTCATGCTTCACAAGCTCCCAAATCTCACCGGTCCCATCCAACCCACCACAGCAAAACTTCACAACCTTAAAATCCTCGACCTCAGCTGGAACAGTCTATCCGGTCCCATCCCTGATTTCCTCAGCACTCTCACCAATCTCTTTGTCCTCAGCCTCTCCTTCAACAACCTCATCGGAACCATCCCGAGTTCCCTCTCAAAACTCCCAAATCTCGGCGGTCTCCAATTGGACCGAAACAAATTAACCGGACAAATCCCAGATTCCTTCGGGTACTTCCCCGGAGAACAGGCCCCGTCTTTGTATCTTTCACACAACCAACTTTCTGGGAAAATCCCATCTTCCTTGTCGAGATTGAACTTAACTTCCATCGATTTCTCCCGCAACAAGCTCGTAGGCGATGCGTTTATGATATTTGGTTCGAATAAAACCGTCCAGATTGTTGATTTGTCGAGAAACCTGTTGGAGTTTAACTTGTCGGAGGTTGTGTTTCCTCAGAGCTTGACTTCGTTGGATCTTAATCATAATAAGGTCTTTGGGGGTTTGTCGCCGGAGATGATGAAGCTGAATTTTCAGTATTTGAATGTGAGTTATAATAGGCTGTGTGGTGAGATTCCTGTTGGTGGAGAGTTGCAGAAGTTTGATGTGTATTCTTATTTTCACAATAAGTGTTTGTGCGGCGCACCGCTTGGTAGCTGCAAATGATGGAGATGGGAACTTATCTCCAAGTTCCCAACTTTATTATTACCTCATTCTTTACACGCAACTTTGAGGATCAAATAAAGTCTGCTCGTTGAATTTTAACTGGGATTTGAAATGAGTTTCTTCGATGCAACTTATTAATTAAAAAGGTGTAGTCGATGGTATTTAGAGATGAATTTATGTTTAATTTAGGATAAATTACTATTTTTGTCAATTTTGGGATTTATTCAATTTTTAGTGTTTGCAATTTTAGACTTCAAATTGAGTTCATGTAACTTTCAATAAAACTTGATGTACATTCTCTTTTAGAAATTTGAAAAAACATATTGACATTGTGC

mRNA sequence

CACACATTCAAACACATCTTTAAAAATCGAAACCAAATCTCCAAAACTTACTCACTGAAGTCCATGGCTTCCAATCTCAAACTCCCATGGCTGCCCCTCTTTCTCTTGGTCTTCTTCATCTTCATCACCATTTCTTTTGCAGAACTCTGCAACCCAAATGACAAAAAAGCCCTCCTCAATATCAAAAAAGCCTTCAACAATCCTTACATCCTCACCTCCTGGGACCCTGAAACCGACTGCTGCCACTGGTACTGTGTCAAGTGCAATCGAACCACTCACCGGATCACTTCCCTTACCATCTTCGCCGACGACCGACTCTCCGGCCAAATCCCACCGGAAGTCGGCGACCTTCCATTTTTAGAAACTCTCATGCTTCACAAGCTCCCAAATCTCACCGGTCCCATCCAACCCACCACAGCAAAACTTCACAACCTTAAAATCCTCGACCTCAGCTGGAACAGTCTATCCGGTCCCATCCCTGATTTCCTCAGCACTCTCACCAATCTCTTTGTCCTCAGCCTCTCCTTCAACAACCTCATCGGAACCATCCCGAGTTCCCTCTCAAAACTCCCAAATCTCGGCGGTCTCCAATTGGACCGAAACAAATTAACCGGACAAATCCCAGATTCCTTCGGGTACTTCCCCGGAGAACAGGCCCCGTCTTTGTATCTTTCACACAACCAACTTTCTGGGAAAATCCCATCTTCCTTGTCGAGATTGAACTTAACTTCCATCGATTTCTCCCGCAACAAGCTCGTAGGCGATGCGTTTATGATATTTGGTTCGAATAAAACCGTCCAGATTGTTGATTTGTCGAGAAACCTGTTGGAGTTTAACTTGTCGGAGGTTGTGTTTCCTCAGAGCTTGACTTCGTTGGATCTTAATCATAATAAGGTCTTTGGGGGTTTGTCGCCGGAGATGATGAAGCTGAATTTTCAGTATTTGAATGTGAGTTATAATAGGCTGTGTGGTGAGATTCCTGTTGGTGGAGAGTTGCAGAAGTTTGATGTGTATTCTTATTTTCACAATAAGTGTTTGTGCGGCGCACCGCTTGGTAGCTGCAAATGATGGAGATGGGAACTTATCTCCAAGTTCCCAACTTTATTATTACCTCATTCTTTACACGCAACTTTGAGGATCAAATAAAGTCTGCTCGTTGAATTTTAACTGGGATTTGAAATGAGTTTCTTCGATGCAACTTATTAATTAAAAAGGTGTAGTCGATGGTATTTAGAGATGAATTTATGTTTAATTTAGGATAAATTACTATTTTTGTCAATTTTGGGATTTATTCAATTTTTAGTGTTTGCAATTTTAGACTTCAAATTGAGTTCATGTAACTTTCAATAAAACTTGATGTACATTCTCTTTTAGAAATTTGAAAAAACATATTGACATTGTGC

Coding sequence (CDS)

ATGGCTTCCAATCTCAAACTCCCATGGCTGCCCCTCTTTCTCTTGGTCTTCTTCATCTTCATCACCATTTCTTTTGCAGAACTCTGCAACCCAAATGACAAAAAAGCCCTCCTCAATATCAAAAAAGCCTTCAACAATCCTTACATCCTCACCTCCTGGGACCCTGAAACCGACTGCTGCCACTGGTACTGTGTCAAGTGCAATCGAACCACTCACCGGATCACTTCCCTTACCATCTTCGCCGACGACCGACTCTCCGGCCAAATCCCACCGGAAGTCGGCGACCTTCCATTTTTAGAAACTCTCATGCTTCACAAGCTCCCAAATCTCACCGGTCCCATCCAACCCACCACAGCAAAACTTCACAACCTTAAAATCCTCGACCTCAGCTGGAACAGTCTATCCGGTCCCATCCCTGATTTCCTCAGCACTCTCACCAATCTCTTTGTCCTCAGCCTCTCCTTCAACAACCTCATCGGAACCATCCCGAGTTCCCTCTCAAAACTCCCAAATCTCGGCGGTCTCCAATTGGACCGAAACAAATTAACCGGACAAATCCCAGATTCCTTCGGGTACTTCCCCGGAGAACAGGCCCCGTCTTTGTATCTTTCACACAACCAACTTTCTGGGAAAATCCCATCTTCCTTGTCGAGATTGAACTTAACTTCCATCGATTTCTCCCGCAACAAGCTCGTAGGCGATGCGTTTATGATATTTGGTTCGAATAAAACCGTCCAGATTGTTGATTTGTCGAGAAACCTGTTGGAGTTTAACTTGTCGGAGGTTGTGTTTCCTCAGAGCTTGACTTCGTTGGATCTTAATCATAATAAGGTCTTTGGGGGTTTGTCGCCGGAGATGATGAAGCTGAATTTTCAGTATTTGAATGTGAGTTATAATAGGCTGTGTGGTGAGATTCCTGTTGGTGGAGAGTTGCAGAAGTTTGATGTGTATTCTTATTTTCACAATAAGTGTTTGTGCGGCGCACCGCTTGGTAGCTGCAAATGA

Protein sequence

MASNLKLPWLPLFLLVFFIFITISFAELCNPNDKKALLNIKKAFNNPYILTSWDPETDCCHWYCVKCNRTTHRITSLTIFADDRLSGQIPPEVGDLPFLETLMLHKLPNLTGPIQPTTAKLHNLKILDLSWNSLSGPIPDFLSTLTNLFVLSLSFNNLIGTIPSSLSKLPNLGGLQLDRNKLTGQIPDSFGYFPGEQAPSLYLSHNQLSGKIPSSLSRLNLTSIDFSRNKLVGDAFMIFGSNKTVQIVDLSRNLLEFNLSEVVFPQSLTSLDLNHNKVFGGLSPEMMKLNFQYLNVSYNRLCGEIPVGGELQKFDVYSYFHNKCLCGAPLGSCK
Homology
BLAST of Tan0020796 vs. ExPASy Swiss-Prot
Match: Q05091 (Polygalacturonase inhibitor OS=Pyrus communis OX=23211 GN=PGIP PE=1 SV=1)

HSP 1 Score: 453.8 bits (1166), Expect = 1.7e-126
Identity = 221/320 (69.06%), Postives = 255/320 (79.69%), Query Frame = 0

Query: 15  LVFFIFITISFAELCNPNDKKALLNIKKAFNNPYILTSWDPETDCCHWYCVKCNRTTHRI 74
           L+F   +  + ++LCNP+DKK LL IKKAF +PY+L SW  +TDCC WYCV C+ TT+RI
Sbjct: 13  LLFSSVLNPALSDLCNPDDKKVLLQIKKAFGDPYVLASWKSDTDCCDWYCVTCDSTTNRI 72

Query: 75  TSLTIFADDRLSGQIPPEVGDLPFLETLMLHKLPNLTGPIQPTTAKLHNLKILDLSWNSL 134
            SLTIFA  ++SGQIP  VGDLP+LETL  HK PNLTGPIQP  AKL  LK L LSW +L
Sbjct: 73  NSLTIFA-GQVSGQIPALVGDLPYLETLEFHKQPNLTGPIQPAIAKLKGLKSLRLSWTNL 132

Query: 135 SGPIPDFLSTLTNLFVLSLSFNNLIGTIPSSLSKLPNLGGLQLDRNKLTGQIPDSFGYFP 194
           SG +PDFLS L NL  L LSFNNL G IPSSLS+LPNLG L+LDRNKLTG IP SFG F 
Sbjct: 133 SGSVPDFLSQLKNLTFLDLSFNNLTGAIPSSLSELPNLGALRLDRNKLTGHIPISFGQFI 192

Query: 195 GEQAPSLYLSHNQLSGKIPSSLSRLNLTSIDFSRNKLVGDAFMIFGSNKTVQIVDLSRNL 254
           G   P LYLSHNQLSG IP+S ++++ TSID SRNKL GDA +IFG NKT QIVDLSRNL
Sbjct: 193 G-NVPDLYLSHNQLSGNIPTSFAQMDFTSIDLSRNKLEGDASVIFGLNKTTQIVDLSRNL 252

Query: 255 LEFNLSEVVFPQSLTSLDLNHNKVFGGLSPEMMKLNFQYLNVSYNRLCGEIPVGGELQKF 314
           LEFNLS+V FP SLTSLD+NHNK++G +  E  +LNFQ+LNVSYNRLCG+IPVGG+LQ F
Sbjct: 253 LEFNLSKVEFPTSLTSLDINHNKIYGSIPVEFTQLNFQFLNVSYNRLCGQIPVGGKLQSF 312

Query: 315 DVYSYFHNKCLCGAPLGSCK 335
           D YSYFHN+CLCGAPL SCK
Sbjct: 313 DEYSYFHNRCLCGAPLPSCK 330

BLAST of Tan0020796 vs. ExPASy Swiss-Prot
Match: A7PW81 (Polygalacturonase inhibitor OS=Vitis vinifera OX=29760 GN=pgip PE=1 SV=1)

HSP 1 Score: 416.0 bits (1068), Expect = 4.0e-115
Identity = 205/311 (65.92%), Postives = 234/311 (75.24%), Query Frame = 0

Query: 24  SFAELCNPNDKKALLNIKKAFNNPYILTSWDPETDCCHWYCVKCNRTTHRITSLTIFADD 83
           S +E CNP DKK LL IKKA +NPYIL SW+P TDCC WYCV+C+ TTHRI SLTIF+  
Sbjct: 25  SLSERCNPKDKKVLLQIKKALDNPYILASWNPNTDCCGWYCVECDLTTHRINSLTIFS-G 84

Query: 84  RLSGQIPPEVGDLPFLETLMLHKLPNLTGPIQPTTAKLHNLKILDLSWNSLSGPIPDFLS 143
           +LSGQIP  VGDLPFLETL+  KL NLTG I P  AKL +LK++ LSW +LSGP+P F S
Sbjct: 85  QLSGQIPDAVGDLPFLETLIFRKLSNLTGQIPPAIAKLKHLKMVRLSWTNLSGPVPAFFS 144

Query: 144 TLTNLFVLSLSFNNLIGTIPSSLSKLPNLGGLQLDRNKLTGQIPDSFGYFPGEQAPSLYL 203
            L NL  L LSFNNL G IP SLS LPNLG L LDRN LTG IPDSFG F G   P L+L
Sbjct: 145 ELKNLTYLDLSFNNLSGPIPGSLSLLPNLGALHLDRNHLTGPIPDSFGKFAG-STPGLHL 204

Query: 204 SHNQLSGKIPSSLSRLNLTSIDFSRNKLVGDAFMIFGSNKTVQIVDLSRNLLEFNLSEVV 263
           SHNQLSGKIP S    +   +D SRNKL GD  + F +NK+ QIVD SRNL +F+LS V 
Sbjct: 205 SHNQLSGKIPYSFRGFDPNVMDLSRNKLEGDLSIFFNANKSTQIVDFSRNLFQFDLSRVE 264

Query: 264 FPQSLTSLDLNHNKVFGGLSPEMMKLNFQYLNVSYNRLCGEIPVGGELQKFDVYSYFHNK 323
           FP+SLTSLDL+HNK+ G L   M  L+ Q+LNVSYNRLCG+IPVGG+LQ FD  SYFHN+
Sbjct: 265 FPKSLTSLDLSHNKIAGSLPEMMTSLDLQFLNVSYNRLCGKIPVGGKLQSFDYDSYFHNR 324

Query: 324 CLCGAPLGSCK 335
           CLCGAPL SCK
Sbjct: 325 CLCGAPLQSCK 333

BLAST of Tan0020796 vs. ExPASy Swiss-Prot
Match: Q9M5J9 (Polygalacturonase inhibitor 1 OS=Arabidopsis thaliana OX=3702 GN=PGIP1 PE=1 SV=1)

HSP 1 Score: 403.7 bits (1036), Expect = 2.1e-111
Identity = 199/326 (61.04%), Postives = 241/326 (73.93%), Query Frame = 0

Query: 12  LFLLVFFIFITISFA-ELCNPNDKKALLNIKKAFNNPYILTSWDPETDCCHWYCVKCNRT 71
           L LL  F F+T   + +LCN NDK  LL IKK+ NNPY L SWDP+TDCC WYC++C   
Sbjct: 7   LCLLFLFTFLTTCLSKDLCNQNDKNTLLKIKKSLNNPYHLASWDPQTDCCSWYCLECGDA 66

Query: 72  T--HRITSLTIFADDRLSGQIPPEVGDLPFLETLMLHKLPNLTGPIQPTTAKLHNLKILD 131
           T  HR+T+LTIF+  ++SGQIP EVGDLP+LETL+  KL NLTG IQPT AKL NL++L 
Sbjct: 67  TVNHRVTALTIFS-GQISGQIPAEVGDLPYLETLVFRKLSNLTGTIQPTIAKLKNLRMLR 126

Query: 132 LSWNSLSGPIPDFLSTLTNLFVLSLSFNNLIGTIPSSLSKLPNLGGLQLDRNKLTGQIPD 191
           LSW +L+GPIPDF+S L NL  L LSFN+L G+IPSSLS LP +  L+L RNKLTG IP+
Sbjct: 127 LSWTNLTGPIPDFISQLKNLEFLELSFNDLSGSIPSSLSTLPKILALELSRNKLTGSIPE 186

Query: 192 SFGYFPGEQAPSLYLSHNQLSGKIPSSLSRLNLTSIDFSRNKLVGDAFMIFGSNKTVQIV 251
           SFG FPG   P L LSHNQLSG IP SL  ++   ID SRNKL GDA M+FGSNKT   +
Sbjct: 187 SFGSFPG-TVPDLRLSHNQLSGPIPKSLGNIDFNRIDLSRNKLQGDASMLFGSNKTTWSI 246

Query: 252 DLSRNLLEFNLSEVVFPQSLTSLDLNHNKVFGGLSPEMMKLNFQYLNVSYNRLCGEIPVG 311
           DLSRN+ +F++S+V  P++L  LDLNHN + G +  +  +   Q+ NVSYN+LCG IP G
Sbjct: 247 DLSRNMFQFDISKVDIPKTLGILDLNHNGITGNIPVQWTEAPLQFFNVSYNKLCGHIPTG 306

Query: 312 GELQKFDVYSYFHNKCLCGAPLGSCK 335
           G+LQ FD YSYFHNKCLCGAPL  CK
Sbjct: 307 GKLQTFDSYSYFHNKCLCGAPLEICK 330

BLAST of Tan0020796 vs. ExPASy Swiss-Prot
Match: Q9M5J8 (Polygalacturonase inhibitor 2 OS=Arabidopsis thaliana OX=3702 GN=PGIP2 PE=2 SV=2)

HSP 1 Score: 388.7 bits (997), Expect = 6.9e-107
Identity = 195/328 (59.45%), Postives = 240/328 (73.17%), Query Frame = 0

Query: 10  LPLFLLVFFIFITISFA-ELCNPNDKKALLNIKKAFNNPYILTSWDPETDCCHWYCVKCN 69
           + LFLL+  + +T S A +LC+ +DK  LL IKK+ NNPY L SWDP+TDCC WYC++C 
Sbjct: 5   MTLFLLLSTLLLTTSLAKDLCHKDDKTTLLKIKKSLNNPYHLASWDPKTDCCSWYCLECG 64

Query: 70  RTT--HRITSLTIFADDRLSGQIPPEVGDLPFLETLMLHKLPNLTGPIQPTTAKLHNLKI 129
             T  HR+TSL I  D  +SGQIPPEVGDLP+L +L+  KL NLTG IQPT AKL NL  
Sbjct: 65  DATVNHRVTSL-IIQDGEISGQIPPEVGDLPYLTSLIFRKLTNLTGHIQPTIAKLKNLTF 124

Query: 130 LDLSWNSLSGPIPDFLSTLTNLFVLSLSFNNLIGTIPSSLSKLPNLGGLQLDRNKLTGQI 189
           L LSW +L+GP+P+FLS L NL  + LSFN+L G+IPSSLS L  L  L+L RNKLTG I
Sbjct: 125 LRLSWTNLTGPVPEFLSQLKNLEYIDLSFNDLSGSIPSSLSSLRKLEYLELSRNKLTGPI 184

Query: 190 PDSFGYFPGEQAPSLYLSHNQLSGKIPSSLSRLNLTSIDFSRNKLVGDAFMIFGSNKTVQ 249
           P+SFG F G + PSL+LSHNQLSG IP SL   +   ID SRNKL GDA ++FG+ KT  
Sbjct: 185 PESFGTFSG-KVPSLFLSHNQLSGTIPKSLGNPDFYRIDLSRNKLQGDASILFGAKKTTW 244

Query: 250 IVDLSRNLLEFNLSEVVFPQSLTSLDLNHNKVFGGLSPEMMKLNFQYLNVSYNRLCGEIP 309
           IVD+SRN+ +F+LS+V   ++L +LD+NHN + G +  E  K  FQ LNVSYNRLCG IP
Sbjct: 245 IVDISRNMFQFDLSKVKLAKTLNNLDMNHNGITGSIPAEWSKAYFQLLNVSYNRLCGRIP 304

Query: 310 VGGELQKFDVYSYFHNKCLCGAPLGSCK 335
            G  +Q+FD YS+FHNKCLCGAPL SCK
Sbjct: 305 KGEYIQRFDSYSFFHNKCLCGAPLPSCK 330

BLAST of Tan0020796 vs. ExPASy Swiss-Prot
Match: Q9LH52 (Leucine-rich repeat protein FLOR 1 OS=Arabidopsis thaliana OX=3702 GN=FLR1 PE=1 SV=1)

HSP 1 Score: 313.2 bits (801), Expect = 3.7e-84
Identity = 165/323 (51.08%), Postives = 216/323 (66.87%), Query Frame = 0

Query: 14  LLVFF--IFITISFAELCNPNDKKALLNIKKAFNNPYILTSWDPETDCC-HWYCVKCNRT 73
           L +FF  +FIT+  +  C  NDK ALL IKKA  NP +L+SW+P TDCC  W  V+C  T
Sbjct: 7   LSIFFSILFITLPSSYSCTENDKNALLQIKKALGNPPLLSSWNPRTDCCTGWTGVEC--T 66

Query: 74  THRITSLTIFADDRLSGQIPPEVGDLPFLETLMLHKLPNLTGPIQPTTAKLHNLKILDLS 133
             R+T L++ + + +SGQI  ++GDL  L TL    LP+LTG I  T  KL NL  L L 
Sbjct: 67  NRRVTGLSVTSGE-VSGQISYQIGDLVDLRTLDFSYLPHLTGNIPRTITKLKNLNTLYLK 126

Query: 134 WNSLSGPIPDFLSTLTNLFVLSLSFNNLIGTIPSSLSKLPNLGGLQLDRNKLTGQIPDSF 193
             SLSGPIPD++S L +L  L LSFN   G IP SLS++P L  +Q++ NKLTG IP+SF
Sbjct: 127 HTSLSGPIPDYISELKSLTFLDLSFNQFTGPIPGSLSQMPKLEAIQINDNKLTGSIPNSF 186

Query: 194 GYFPGEQAPSLYLSHNQLSGKIPSSLSRLNLTSIDFSRNKLVGDAFMIFGSNKTVQIVDL 253
           G F G   P+LYLS+N+LSGKIP SLS+ +  ++D S N   GDAFM FG NKT   VDL
Sbjct: 187 GSFVG-NVPNLYLSNNKLSGKIPESLSKYDFNAVDLSGNGFEGDAFMFFGRNKTTVRVDL 246

Query: 254 SRNLLEFNLSEVVFPQSLTSLDLNHNKVFGGLSPEMMKLNFQYLNVSYNRLCGEIPVGGE 313
           SRN+  F+L +V F +S+ SLDL+ N ++G + P + KL+ ++ NVS N LCG+IP GG 
Sbjct: 247 SRNMFNFDLVKVKFARSIVSLDLSQNHIYGKIPPALTKLHLEHFNVSDNHLCGKIPSGGL 306

Query: 314 LQKFDVYSYFHNKCLCGAPLGSC 334
           LQ F+  ++ HN CLCG PL +C
Sbjct: 307 LQTFEPSAFAHNICLCGTPLKAC 325

BLAST of Tan0020796 vs. NCBI nr
Match: XP_023517351.1 (polygalacturonase inhibitor-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 607.8 bits (1566), Expect = 5.5e-170
Identity = 297/334 (88.92%), Postives = 305/334 (91.32%), Query Frame = 0

Query: 1   MASNLKLPWLPLFLLVFFIFITISFAELCNPNDKKALLNIKKAFNNPYILTSWDPETDCC 60
           MASNL LP     L +F IFI IS AELC+ +DKKALLNIKKAFNNPYILTSWDP+TDCC
Sbjct: 1   MASNLNLP----CLFLFSIFIAISVAELCHQDDKKALLNIKKAFNNPYILTSWDPQTDCC 60

Query: 61  HWYCVKCNRTTHRITSLTIFADDRLSGQIPPEVGDLPFLETLMLHKLPNLTGPIQPTTAK 120
           HWYCVKCNRTTHRITSLTIFADDRLSGQIPP VGDLPFLETLMLHKLPNLTGPI P  AK
Sbjct: 61  HWYCVKCNRTTHRITSLTIFADDRLSGQIPPYVGDLPFLETLMLHKLPNLTGPIHPNIAK 120

Query: 121 LHNLKILDLSWNSLSGPIPDFLSTLTNLFVLSLSFNNLIGTIPSSLSKLPNLGGLQLDRN 180
           LHNLK LDLSWNSLSGPIPDFLSTL+NLFVLSLSFNN  GTIP SLSKLPNLGGL+LDRN
Sbjct: 121 LHNLKFLDLSWNSLSGPIPDFLSTLSNLFVLSLSFNNFNGTIPISLSKLPNLGGLELDRN 180

Query: 181 KLTGQIPDSFGYFPGEQAPSLYLSHNQLSGKIPSSLSRLNLTSIDFSRNKLVGDAFMIFG 240
            LTG+IPDSFGYF GEQAPSLYLSHNQLSGKIPSSLSRLN TSID SRNKL GDAFMIFG
Sbjct: 181 NLTGEIPDSFGYFTGEQAPSLYLSHNQLSGKIPSSLSRLNFTSIDLSRNKLKGDAFMIFG 240

Query: 241 SNKTVQIVDLSRNLLEFNLSEVVFPQSLTSLDLNHNKVFGGLSPEMMKLNFQYLNVSYNR 300
           SNKTVQIVDLSRN LEFNLSE VFPQSLTSLD+NHNKVFGGL PEM KLNFQYLNVSYNR
Sbjct: 241 SNKTVQIVDLSRNQLEFNLSEAVFPQSLTSLDVNHNKVFGGLPPEMTKLNFQYLNVSYNR 300

Query: 301 LCGEIPVGGELQKFDVYSYFHNKCLCGAPLGSCK 335
           LCGEIPVGGELQKFDVY YFHNKCLCGAPLGSCK
Sbjct: 301 LCGEIPVGGELQKFDVYEYFHNKCLCGAPLGSCK 330

BLAST of Tan0020796 vs. NCBI nr
Match: KAG6595658.1 (Polygalacturonase inhibitor, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 607.4 bits (1565), Expect = 7.2e-170
Identity = 295/334 (88.32%), Postives = 307/334 (91.92%), Query Frame = 0

Query: 1   MASNLKLPWLPLFLLVFFIFITISFAELCNPNDKKALLNIKKAFNNPYILTSWDPETDCC 60
           MA NLKLPWL +FL  F I I IS AELC+ +DKKALLNIKKAFNNPYILTSWDP+TDCC
Sbjct: 1   MAPNLKLPWLYIFL--FSISIAISVAELCHQDDKKALLNIKKAFNNPYILTSWDPQTDCC 60

Query: 61  HWYCVKCNRTTHRITSLTIFADDRLSGQIPPEVGDLPFLETLMLHKLPNLTGPIQPTTAK 120
           HWYCVKCNRTTHRITSLTIFADDRL+GQIPP VGDLPFLETL+LHKLPNLTGPI P+ AK
Sbjct: 61  HWYCVKCNRTTHRITSLTIFADDRLTGQIPPYVGDLPFLETLILHKLPNLTGPIHPSIAK 120

Query: 121 LHNLKILDLSWNSLSGPIPDFLSTLTNLFVLSLSFNNLIGTIPSSLSKLPNLGGLQLDRN 180
           LHNLK LDLSWNSLSGPIPDFLSTL+NLFVLSLSFNN  GTIP+SLSKLPNLGGL+LDRN
Sbjct: 121 LHNLKYLDLSWNSLSGPIPDFLSTLSNLFVLSLSFNNFNGTIPNSLSKLPNLGGLELDRN 180

Query: 181 KLTGQIPDSFGYFPGEQAPSLYLSHNQLSGKIPSSLSRLNLTSIDFSRNKLVGDAFMIFG 240
            LTG+IPDSFGYF GEQ PSL+LSHNQLSGKIPSSLSRLN TSID SRNKL GDAFMIFG
Sbjct: 181 NLTGEIPDSFGYFTGEQGPSLFLSHNQLSGKIPSSLSRLNFTSIDLSRNKLKGDAFMIFG 240

Query: 241 SNKTVQIVDLSRNLLEFNLSEVVFPQSLTSLDLNHNKVFGGLSPEMMKLNFQYLNVSYNR 300
            NKTVQIVDLSRN LEFNLSE VFPQSLTSLDLNHNKVFGGL PEM KLNFQYLNVSYNR
Sbjct: 241 LNKTVQIVDLSRNQLEFNLSEAVFPQSLTSLDLNHNKVFGGLPPEMTKLNFQYLNVSYNR 300

Query: 301 LCGEIPVGGELQKFDVYSYFHNKCLCGAPLGSCK 335
           LCGEIPVGGELQKFDVY YFHNKCLCGAPLGSCK
Sbjct: 301 LCGEIPVGGELQKFDVYEYFHNKCLCGAPLGSCK 332

BLAST of Tan0020796 vs. NCBI nr
Match: XP_022966389.1 (polygalacturonase inhibitor-like [Cucurbita maxima])

HSP 1 Score: 604.4 bits (1557), Expect = 6.1e-169
Identity = 296/334 (88.62%), Postives = 305/334 (91.32%), Query Frame = 0

Query: 1   MASNLKLPWLPLFLLVFFIFITISFAELCNPNDKKALLNIKKAFNNPYILTSWDPETDCC 60
           MAS L LP L L L     FITIS AELC+ +DKKALLNIKKAFNNPYILTSWDP+TDCC
Sbjct: 1   MASKLNLPCLFLSL-----FITISVAELCHQDDKKALLNIKKAFNNPYILTSWDPQTDCC 60

Query: 61  HWYCVKCNRTTHRITSLTIFADDRLSGQIPPEVGDLPFLETLMLHKLPNLTGPIQPTTAK 120
           HWYCVKCNRTTHRITSLTIFADDRLSG+IPP VGDLPFLETL+LHKLPNLTGPI P  AK
Sbjct: 61  HWYCVKCNRTTHRITSLTIFADDRLSGEIPPYVGDLPFLETLILHKLPNLTGPIHPNIAK 120

Query: 121 LHNLKILDLSWNSLSGPIPDFLSTLTNLFVLSLSFNNLIGTIPSSLSKLPNLGGLQLDRN 180
           LHNLK LDLSWNSLSGPIPDFLSTL+NLFVLSLSFNN  GTIP+SLSKLPNLGGLQLDRN
Sbjct: 121 LHNLKFLDLSWNSLSGPIPDFLSTLSNLFVLSLSFNNFNGTIPNSLSKLPNLGGLQLDRN 180

Query: 181 KLTGQIPDSFGYFPGEQAPSLYLSHNQLSGKIPSSLSRLNLTSIDFSRNKLVGDAFMIFG 240
            LTG+IPDSFGYF GEQAPSLYLSHNQLSGKIPSSLSRLN TSID SRNKL GDAFMIFG
Sbjct: 181 NLTGEIPDSFGYFTGEQAPSLYLSHNQLSGKIPSSLSRLNFTSIDLSRNKLKGDAFMIFG 240

Query: 241 SNKTVQIVDLSRNLLEFNLSEVVFPQSLTSLDLNHNKVFGGLSPEMMKLNFQYLNVSYNR 300
           SNKTVQIVDLSRN LEFNLSE VFPQSLTSLD+NHNKVFGGL PEM KLNFQYLNVSYNR
Sbjct: 241 SNKTVQIVDLSRNQLEFNLSEAVFPQSLTSLDVNHNKVFGGLRPEMTKLNFQYLNVSYNR 300

Query: 301 LCGEIPVGGELQKFDVYSYFHNKCLCGAPLGSCK 335
           LCGEIPVGGELQKFDVY YFHNKCLCGAPLGSCK
Sbjct: 301 LCGEIPVGGELQKFDVYEYFHNKCLCGAPLGSCK 329

BLAST of Tan0020796 vs. NCBI nr
Match: XP_022925134.1 (polygalacturonase inhibitor-like [Cucurbita moschata])

HSP 1 Score: 602.8 bits (1553), Expect = 1.8e-168
Identity = 294/333 (88.29%), Postives = 304/333 (91.29%), Query Frame = 0

Query: 1   MASNLKLPWLPLFLLVFFIFITISFAELCNPNDKKALLNIKKAFNNPYILTSWDPETDCC 60
           MAS L L WL LFL  F I I IS AELC+ +DKKALLNIKKAFNNPYILTSWDP+TDCC
Sbjct: 1   MASKLNLRWLSLFL--FSISIAISVAELCHQDDKKALLNIKKAFNNPYILTSWDPQTDCC 60

Query: 61  HWYCVKCNRTTHRITSLTIFADDRLSGQIPPEVGDLPFLETLMLHKLPNLTGPIQPTTAK 120
           HWYCVKCNRTTHRITSLTIFADDRLSGQIPP VGDLPFLETL+LHKLPNLTGPI P  AK
Sbjct: 61  HWYCVKCNRTTHRITSLTIFADDRLSGQIPPYVGDLPFLETLILHKLPNLTGPIHPNIAK 120

Query: 121 LHNLKILDLSWNSLSGPIPDFLSTLTNLFVLSLSFNNLIGTIPSSLSKLPNLGGLQLDRN 180
           LHNLK LDLSWNSLSGPIPDFLSTL+NLFVLSLSFNN  GTIP+SLSKLPNLGGL+LDRN
Sbjct: 121 LHNLKYLDLSWNSLSGPIPDFLSTLSNLFVLSLSFNNFNGTIPNSLSKLPNLGGLELDRN 180

Query: 181 KLTGQIPDSFGYFPGEQAPSLYLSHNQLSGKIPSSLSRLNLTSIDFSRNKLVGDAFMIFG 240
            LTG+IPDSFGYF GEQ PSL+LSHNQLSGKIPSSLSRLN TSID SRNKL GDAFMIFG
Sbjct: 181 NLTGEIPDSFGYFTGEQGPSLFLSHNQLSGKIPSSLSRLNFTSIDLSRNKLKGDAFMIFG 240

Query: 241 SNKTVQIVDLSRNLLEFNLSEVVFPQSLTSLDLNHNKVFGGLSPEMMKLNFQYLNVSYNR 300
           SNKTVQIVDLSRN LEFNLSE VFPQSLTSLD+NHNKVFGGL PEM KLNFQYLNVSYNR
Sbjct: 241 SNKTVQIVDLSRNQLEFNLSEAVFPQSLTSLDVNHNKVFGGLRPEMTKLNFQYLNVSYNR 300

Query: 301 LCGEIPVGGELQKFDVYSYFHNKCLCGAPLGSC 334
           LCGEIPVGGELQKFDVY YFHNKCLCGAPLGSC
Sbjct: 301 LCGEIPVGGELQKFDVYEYFHNKCLCGAPLGSC 331

BLAST of Tan0020796 vs. NCBI nr
Match: KAA0044799.1 (polygalacturonase inhibitor-like [Cucumis melo var. makuwa])

HSP 1 Score: 589.0 bits (1517), Expect = 2.6e-164
Identity = 285/335 (85.07%), Postives = 300/335 (89.55%), Query Frame = 0

Query: 1   MASNL-KLPWLPLFLLVFFIFITISFAELCNPNDKKALLNIKKAFNNPYILTSWDPETDC 60
           MASNL KL WLP F   FF FITISFAELCNPNDKK LL IKKAFNNPYILTSWDP+TDC
Sbjct: 1   MASNLNKLSWLPFF---FFFFITISFAELCNPNDKKVLLKIKKAFNNPYILTSWDPQTDC 60

Query: 61  CHWYCVKCNRTTHRITSLTIFADDRLSGQIPPEVGDLPFLETLMLHKLPNLTGPIQPTTA 120
           CHWYCVKC+RTTHRI SLTIFADDRL+GQIPPEVGDLPFL+TLMLHKLPNLTGPIQPT A
Sbjct: 61  CHWYCVKCDRTTHRIISLTIFADDRLTGQIPPEVGDLPFLQTLMLHKLPNLTGPIQPTIA 120

Query: 121 KLHNLKILDLSWNSLSGPIPDFLSTLTNLFVLSLSFNNLIGTIPSSLSKLPNLGGLQLDR 180
           KLHNLK LDLSWN LSG IPD LSTL NLF+L+LSFN L G IPSSLS+LP+LGGL+LDR
Sbjct: 121 KLHNLKFLDLSWNGLSGEIPDSLSTLKNLFILTLSFNKLTGKIPSSLSELPSLGGLRLDR 180

Query: 181 NKLTGQIPDSFGYFPGEQAPSLYLSHNQLSGKIPSSLSRLNLTSIDFSRNKLVGDAFMIF 240
           N+LTGQIPDSFGYF GEQAP L LSHNQLSGKIPSSLSRLN TSID SRNKL GDAFM F
Sbjct: 181 NQLTGQIPDSFGYFAGEQAPDLILSHNQLSGKIPSSLSRLNFTSIDLSRNKLEGDAFMFF 240

Query: 241 GSNKTVQIVDLSRNLLEFNLSEVVFPQSLTSLDLNHNKVFGGLSPEMMKLNFQYLNVSYN 300
           G+NKT+QI+DLSRNLLEFNLSEVVFPQSLTSLDLNHNKVFGGL P M +L  QYLNVSYN
Sbjct: 241 GANKTIQILDLSRNLLEFNLSEVVFPQSLTSLDLNHNKVFGGLPPVMTELKLQYLNVSYN 300

Query: 301 RLCGEIPVGGELQKFDVYSYFHNKCLCGAPLGSCK 335
           RLCG IPVGG LQ FD+YSYFHNKCLCGAPLG CK
Sbjct: 301 RLCGPIPVGGNLQSFDLYSYFHNKCLCGAPLGDCK 332

BLAST of Tan0020796 vs. ExPASy TrEMBL
Match: A0A6J1HTN4 (polygalacturonase inhibitor-like OS=Cucurbita maxima OX=3661 GN=LOC111466052 PE=4 SV=1)

HSP 1 Score: 604.4 bits (1557), Expect = 2.9e-169
Identity = 296/334 (88.62%), Postives = 305/334 (91.32%), Query Frame = 0

Query: 1   MASNLKLPWLPLFLLVFFIFITISFAELCNPNDKKALLNIKKAFNNPYILTSWDPETDCC 60
           MAS L LP L L L     FITIS AELC+ +DKKALLNIKKAFNNPYILTSWDP+TDCC
Sbjct: 1   MASKLNLPCLFLSL-----FITISVAELCHQDDKKALLNIKKAFNNPYILTSWDPQTDCC 60

Query: 61  HWYCVKCNRTTHRITSLTIFADDRLSGQIPPEVGDLPFLETLMLHKLPNLTGPIQPTTAK 120
           HWYCVKCNRTTHRITSLTIFADDRLSG+IPP VGDLPFLETL+LHKLPNLTGPI P  AK
Sbjct: 61  HWYCVKCNRTTHRITSLTIFADDRLSGEIPPYVGDLPFLETLILHKLPNLTGPIHPNIAK 120

Query: 121 LHNLKILDLSWNSLSGPIPDFLSTLTNLFVLSLSFNNLIGTIPSSLSKLPNLGGLQLDRN 180
           LHNLK LDLSWNSLSGPIPDFLSTL+NLFVLSLSFNN  GTIP+SLSKLPNLGGLQLDRN
Sbjct: 121 LHNLKFLDLSWNSLSGPIPDFLSTLSNLFVLSLSFNNFNGTIPNSLSKLPNLGGLQLDRN 180

Query: 181 KLTGQIPDSFGYFPGEQAPSLYLSHNQLSGKIPSSLSRLNLTSIDFSRNKLVGDAFMIFG 240
            LTG+IPDSFGYF GEQAPSLYLSHNQLSGKIPSSLSRLN TSID SRNKL GDAFMIFG
Sbjct: 181 NLTGEIPDSFGYFTGEQAPSLYLSHNQLSGKIPSSLSRLNFTSIDLSRNKLKGDAFMIFG 240

Query: 241 SNKTVQIVDLSRNLLEFNLSEVVFPQSLTSLDLNHNKVFGGLSPEMMKLNFQYLNVSYNR 300
           SNKTVQIVDLSRN LEFNLSE VFPQSLTSLD+NHNKVFGGL PEM KLNFQYLNVSYNR
Sbjct: 241 SNKTVQIVDLSRNQLEFNLSEAVFPQSLTSLDVNHNKVFGGLRPEMTKLNFQYLNVSYNR 300

Query: 301 LCGEIPVGGELQKFDVYSYFHNKCLCGAPLGSCK 335
           LCGEIPVGGELQKFDVY YFHNKCLCGAPLGSCK
Sbjct: 301 LCGEIPVGGELQKFDVYEYFHNKCLCGAPLGSCK 329

BLAST of Tan0020796 vs. ExPASy TrEMBL
Match: A0A6J1EB92 (polygalacturonase inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111432468 PE=4 SV=1)

HSP 1 Score: 602.8 bits (1553), Expect = 8.6e-169
Identity = 294/333 (88.29%), Postives = 304/333 (91.29%), Query Frame = 0

Query: 1   MASNLKLPWLPLFLLVFFIFITISFAELCNPNDKKALLNIKKAFNNPYILTSWDPETDCC 60
           MAS L L WL LFL  F I I IS AELC+ +DKKALLNIKKAFNNPYILTSWDP+TDCC
Sbjct: 1   MASKLNLRWLSLFL--FSISIAISVAELCHQDDKKALLNIKKAFNNPYILTSWDPQTDCC 60

Query: 61  HWYCVKCNRTTHRITSLTIFADDRLSGQIPPEVGDLPFLETLMLHKLPNLTGPIQPTTAK 120
           HWYCVKCNRTTHRITSLTIFADDRLSGQIPP VGDLPFLETL+LHKLPNLTGPI P  AK
Sbjct: 61  HWYCVKCNRTTHRITSLTIFADDRLSGQIPPYVGDLPFLETLILHKLPNLTGPIHPNIAK 120

Query: 121 LHNLKILDLSWNSLSGPIPDFLSTLTNLFVLSLSFNNLIGTIPSSLSKLPNLGGLQLDRN 180
           LHNLK LDLSWNSLSGPIPDFLSTL+NLFVLSLSFNN  GTIP+SLSKLPNLGGL+LDRN
Sbjct: 121 LHNLKYLDLSWNSLSGPIPDFLSTLSNLFVLSLSFNNFNGTIPNSLSKLPNLGGLELDRN 180

Query: 181 KLTGQIPDSFGYFPGEQAPSLYLSHNQLSGKIPSSLSRLNLTSIDFSRNKLVGDAFMIFG 240
            LTG+IPDSFGYF GEQ PSL+LSHNQLSGKIPSSLSRLN TSID SRNKL GDAFMIFG
Sbjct: 181 NLTGEIPDSFGYFTGEQGPSLFLSHNQLSGKIPSSLSRLNFTSIDLSRNKLKGDAFMIFG 240

Query: 241 SNKTVQIVDLSRNLLEFNLSEVVFPQSLTSLDLNHNKVFGGLSPEMMKLNFQYLNVSYNR 300
           SNKTVQIVDLSRN LEFNLSE VFPQSLTSLD+NHNKVFGGL PEM KLNFQYLNVSYNR
Sbjct: 241 SNKTVQIVDLSRNQLEFNLSEAVFPQSLTSLDVNHNKVFGGLRPEMTKLNFQYLNVSYNR 300

Query: 301 LCGEIPVGGELQKFDVYSYFHNKCLCGAPLGSC 334
           LCGEIPVGGELQKFDVY YFHNKCLCGAPLGSC
Sbjct: 301 LCGEIPVGGELQKFDVYEYFHNKCLCGAPLGSC 331

BLAST of Tan0020796 vs. ExPASy TrEMBL
Match: A0A5D3CYT3 (Polygalacturonase inhibitor-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold21G004960 PE=4 SV=1)

HSP 1 Score: 589.0 bits (1517), Expect = 1.3e-164
Identity = 285/337 (84.57%), Postives = 301/337 (89.32%), Query Frame = 0

Query: 1   MASNL-KLPWLPLFLL--VFFIFITISFAELCNPNDKKALLNIKKAFNNPYILTSWDPET 60
           MASNL KL WLP F +   FF FITISFAELCNPNDKK LL IKKAFNNPYILTSWDP+T
Sbjct: 1   MASNLNKLSWLPFFFIFFFFFFFITISFAELCNPNDKKVLLKIKKAFNNPYILTSWDPQT 60

Query: 61  DCCHWYCVKCNRTTHRITSLTIFADDRLSGQIPPEVGDLPFLETLMLHKLPNLTGPIQPT 120
           DCCHWYCVKC+RTTHRI SLTIFADDRL+GQIPPEVGDLPFL+TLMLHKLPNLTGPIQPT
Sbjct: 61  DCCHWYCVKCDRTTHRIISLTIFADDRLTGQIPPEVGDLPFLQTLMLHKLPNLTGPIQPT 120

Query: 121 TAKLHNLKILDLSWNSLSGPIPDFLSTLTNLFVLSLSFNNLIGTIPSSLSKLPNLGGLQL 180
            AKLHNLK LDLSWN LSG IPD LSTL NLF+L+LSFN L G IPSSLS+LP+LGGL+L
Sbjct: 121 IAKLHNLKFLDLSWNGLSGEIPDSLSTLKNLFILTLSFNKLTGKIPSSLSELPSLGGLRL 180

Query: 181 DRNKLTGQIPDSFGYFPGEQAPSLYLSHNQLSGKIPSSLSRLNLTSIDFSRNKLVGDAFM 240
           DRN+LTGQIPDSFGYF GEQAP L LSHNQLSGKIPSSLSRLN TSID SRNKL GDAFM
Sbjct: 181 DRNQLTGQIPDSFGYFAGEQAPDLILSHNQLSGKIPSSLSRLNFTSIDLSRNKLEGDAFM 240

Query: 241 IFGSNKTVQIVDLSRNLLEFNLSEVVFPQSLTSLDLNHNKVFGGLSPEMMKLNFQYLNVS 300
            FG+NKT+QI+DLSRNLLEFNLSEVVFPQSLTSLDLNHNKVFGGL P M +L  QYLNVS
Sbjct: 241 FFGANKTIQILDLSRNLLEFNLSEVVFPQSLTSLDLNHNKVFGGLPPVMTELKLQYLNVS 300

Query: 301 YNRLCGEIPVGGELQKFDVYSYFHNKCLCGAPLGSCK 335
           YNRLCG IPVGG LQ FD+YSYFHNKCLCGAPLG CK
Sbjct: 301 YNRLCGPIPVGGNLQSFDLYSYFHNKCLCGAPLGDCK 337

BLAST of Tan0020796 vs. ExPASy TrEMBL
Match: A0A5A7TU82 (Polygalacturonase inhibitor-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold74G00900 PE=4 SV=1)

HSP 1 Score: 589.0 bits (1517), Expect = 1.3e-164
Identity = 285/335 (85.07%), Postives = 300/335 (89.55%), Query Frame = 0

Query: 1   MASNL-KLPWLPLFLLVFFIFITISFAELCNPNDKKALLNIKKAFNNPYILTSWDPETDC 60
           MASNL KL WLP F   FF FITISFAELCNPNDKK LL IKKAFNNPYILTSWDP+TDC
Sbjct: 1   MASNLNKLSWLPFF---FFFFITISFAELCNPNDKKVLLKIKKAFNNPYILTSWDPQTDC 60

Query: 61  CHWYCVKCNRTTHRITSLTIFADDRLSGQIPPEVGDLPFLETLMLHKLPNLTGPIQPTTA 120
           CHWYCVKC+RTTHRI SLTIFADDRL+GQIPPEVGDLPFL+TLMLHKLPNLTGPIQPT A
Sbjct: 61  CHWYCVKCDRTTHRIISLTIFADDRLTGQIPPEVGDLPFLQTLMLHKLPNLTGPIQPTIA 120

Query: 121 KLHNLKILDLSWNSLSGPIPDFLSTLTNLFVLSLSFNNLIGTIPSSLSKLPNLGGLQLDR 180
           KLHNLK LDLSWN LSG IPD LSTL NLF+L+LSFN L G IPSSLS+LP+LGGL+LDR
Sbjct: 121 KLHNLKFLDLSWNGLSGEIPDSLSTLKNLFILTLSFNKLTGKIPSSLSELPSLGGLRLDR 180

Query: 181 NKLTGQIPDSFGYFPGEQAPSLYLSHNQLSGKIPSSLSRLNLTSIDFSRNKLVGDAFMIF 240
           N+LTGQIPDSFGYF GEQAP L LSHNQLSGKIPSSLSRLN TSID SRNKL GDAFM F
Sbjct: 181 NQLTGQIPDSFGYFAGEQAPDLILSHNQLSGKIPSSLSRLNFTSIDLSRNKLEGDAFMFF 240

Query: 241 GSNKTVQIVDLSRNLLEFNLSEVVFPQSLTSLDLNHNKVFGGLSPEMMKLNFQYLNVSYN 300
           G+NKT+QI+DLSRNLLEFNLSEVVFPQSLTSLDLNHNKVFGGL P M +L  QYLNVSYN
Sbjct: 241 GANKTIQILDLSRNLLEFNLSEVVFPQSLTSLDLNHNKVFGGLPPVMTELKLQYLNVSYN 300

Query: 301 RLCGEIPVGGELQKFDVYSYFHNKCLCGAPLGSCK 335
           RLCG IPVGG LQ FD+YSYFHNKCLCGAPLG CK
Sbjct: 301 RLCGPIPVGGNLQSFDLYSYFHNKCLCGAPLGDCK 332

BLAST of Tan0020796 vs. ExPASy TrEMBL
Match: A0A1S3BU59 (polygalacturonase inhibitor-like OS=Cucumis melo OX=3656 GN=LOC103493193 PE=4 SV=1)

HSP 1 Score: 589.0 bits (1517), Expect = 1.3e-164
Identity = 285/337 (84.57%), Postives = 301/337 (89.32%), Query Frame = 0

Query: 1   MASNL-KLPWLPLFLL--VFFIFITISFAELCNPNDKKALLNIKKAFNNPYILTSWDPET 60
           MASNL KL WLP F +   FF FITISFAELCNPNDKK LL IKKAFNNPYILTSWDP+T
Sbjct: 1   MASNLNKLSWLPFFFIFFFFFFFITISFAELCNPNDKKVLLKIKKAFNNPYILTSWDPQT 60

Query: 61  DCCHWYCVKCNRTTHRITSLTIFADDRLSGQIPPEVGDLPFLETLMLHKLPNLTGPIQPT 120
           DCCHWYCVKC+RTTHRI SLTIFADDRL+GQIPPEVGDLPFL+TLMLHKLPNLTGPIQPT
Sbjct: 61  DCCHWYCVKCDRTTHRIISLTIFADDRLTGQIPPEVGDLPFLQTLMLHKLPNLTGPIQPT 120

Query: 121 TAKLHNLKILDLSWNSLSGPIPDFLSTLTNLFVLSLSFNNLIGTIPSSLSKLPNLGGLQL 180
            AKLHNLK LDLSWN LSG IPD LSTL NLF+L+LSFN L G IPSSLS+LP+LGGL+L
Sbjct: 121 IAKLHNLKFLDLSWNGLSGEIPDSLSTLKNLFILTLSFNKLTGKIPSSLSELPSLGGLRL 180

Query: 181 DRNKLTGQIPDSFGYFPGEQAPSLYLSHNQLSGKIPSSLSRLNLTSIDFSRNKLVGDAFM 240
           DRN+LTGQIPDSFGYF GEQAP L LSHNQLSGKIPSSLSRLN TSID SRNKL GDAFM
Sbjct: 181 DRNQLTGQIPDSFGYFAGEQAPDLILSHNQLSGKIPSSLSRLNFTSIDLSRNKLEGDAFM 240

Query: 241 IFGSNKTVQIVDLSRNLLEFNLSEVVFPQSLTSLDLNHNKVFGGLSPEMMKLNFQYLNVS 300
            FG+NKT+QI+DLSRNLLEFNLSEVVFPQSLTSLDLNHNKVFGGL P M +L  QYLNVS
Sbjct: 241 FFGANKTIQILDLSRNLLEFNLSEVVFPQSLTSLDLNHNKVFGGLPPVMTELKLQYLNVS 300

Query: 301 YNRLCGEIPVGGELQKFDVYSYFHNKCLCGAPLGSCK 335
           YNRLCG IPVGG LQ FD+YSYFHNKCLCGAPLG CK
Sbjct: 301 YNRLCGPIPVGGNLQSFDLYSYFHNKCLCGAPLGDCK 337

BLAST of Tan0020796 vs. TAIR 10
Match: AT5G06860.1 (polygalacturonase inhibiting protein 1 )

HSP 1 Score: 403.7 bits (1036), Expect = 1.5e-112
Identity = 199/326 (61.04%), Postives = 241/326 (73.93%), Query Frame = 0

Query: 12  LFLLVFFIFITISFA-ELCNPNDKKALLNIKKAFNNPYILTSWDPETDCCHWYCVKCNRT 71
           L LL  F F+T   + +LCN NDK  LL IKK+ NNPY L SWDP+TDCC WYC++C   
Sbjct: 7   LCLLFLFTFLTTCLSKDLCNQNDKNTLLKIKKSLNNPYHLASWDPQTDCCSWYCLECGDA 66

Query: 72  T--HRITSLTIFADDRLSGQIPPEVGDLPFLETLMLHKLPNLTGPIQPTTAKLHNLKILD 131
           T  HR+T+LTIF+  ++SGQIP EVGDLP+LETL+  KL NLTG IQPT AKL NL++L 
Sbjct: 67  TVNHRVTALTIFS-GQISGQIPAEVGDLPYLETLVFRKLSNLTGTIQPTIAKLKNLRMLR 126

Query: 132 LSWNSLSGPIPDFLSTLTNLFVLSLSFNNLIGTIPSSLSKLPNLGGLQLDRNKLTGQIPD 191
           LSW +L+GPIPDF+S L NL  L LSFN+L G+IPSSLS LP +  L+L RNKLTG IP+
Sbjct: 127 LSWTNLTGPIPDFISQLKNLEFLELSFNDLSGSIPSSLSTLPKILALELSRNKLTGSIPE 186

Query: 192 SFGYFPGEQAPSLYLSHNQLSGKIPSSLSRLNLTSIDFSRNKLVGDAFMIFGSNKTVQIV 251
           SFG FPG   P L LSHNQLSG IP SL  ++   ID SRNKL GDA M+FGSNKT   +
Sbjct: 187 SFGSFPG-TVPDLRLSHNQLSGPIPKSLGNIDFNRIDLSRNKLQGDASMLFGSNKTTWSI 246

Query: 252 DLSRNLLEFNLSEVVFPQSLTSLDLNHNKVFGGLSPEMMKLNFQYLNVSYNRLCGEIPVG 311
           DLSRN+ +F++S+V  P++L  LDLNHN + G +  +  +   Q+ NVSYN+LCG IP G
Sbjct: 247 DLSRNMFQFDISKVDIPKTLGILDLNHNGITGNIPVQWTEAPLQFFNVSYNKLCGHIPTG 306

Query: 312 GELQKFDVYSYFHNKCLCGAPLGSCK 335
           G+LQ FD YSYFHNKCLCGAPL  CK
Sbjct: 307 GKLQTFDSYSYFHNKCLCGAPLEICK 330

BLAST of Tan0020796 vs. TAIR 10
Match: AT5G06870.1 (polygalacturonase inhibiting protein 2 )

HSP 1 Score: 388.7 bits (997), Expect = 4.9e-108
Identity = 195/328 (59.45%), Postives = 240/328 (73.17%), Query Frame = 0

Query: 10  LPLFLLVFFIFITISFA-ELCNPNDKKALLNIKKAFNNPYILTSWDPETDCCHWYCVKCN 69
           + LFLL+  + +T S A +LC+ +DK  LL IKK+ NNPY L SWDP+TDCC WYC++C 
Sbjct: 5   MTLFLLLSTLLLTTSLAKDLCHKDDKTTLLKIKKSLNNPYHLASWDPKTDCCSWYCLECG 64

Query: 70  RTT--HRITSLTIFADDRLSGQIPPEVGDLPFLETLMLHKLPNLTGPIQPTTAKLHNLKI 129
             T  HR+TSL I  D  +SGQIPPEVGDLP+L +L+  KL NLTG IQPT AKL NL  
Sbjct: 65  DATVNHRVTSL-IIQDGEISGQIPPEVGDLPYLTSLIFRKLTNLTGHIQPTIAKLKNLTF 124

Query: 130 LDLSWNSLSGPIPDFLSTLTNLFVLSLSFNNLIGTIPSSLSKLPNLGGLQLDRNKLTGQI 189
           L LSW +L+GP+P+FLS L NL  + LSFN+L G+IPSSLS L  L  L+L RNKLTG I
Sbjct: 125 LRLSWTNLTGPVPEFLSQLKNLEYIDLSFNDLSGSIPSSLSSLRKLEYLELSRNKLTGPI 184

Query: 190 PDSFGYFPGEQAPSLYLSHNQLSGKIPSSLSRLNLTSIDFSRNKLVGDAFMIFGSNKTVQ 249
           P+SFG F G + PSL+LSHNQLSG IP SL   +   ID SRNKL GDA ++FG+ KT  
Sbjct: 185 PESFGTFSG-KVPSLFLSHNQLSGTIPKSLGNPDFYRIDLSRNKLQGDASILFGAKKTTW 244

Query: 250 IVDLSRNLLEFNLSEVVFPQSLTSLDLNHNKVFGGLSPEMMKLNFQYLNVSYNRLCGEIP 309
           IVD+SRN+ +F+LS+V   ++L +LD+NHN + G +  E  K  FQ LNVSYNRLCG IP
Sbjct: 245 IVDISRNMFQFDLSKVKLAKTLNNLDMNHNGITGSIPAEWSKAYFQLLNVSYNRLCGRIP 304

Query: 310 VGGELQKFDVYSYFHNKCLCGAPLGSCK 335
            G  +Q+FD YS+FHNKCLCGAPL SCK
Sbjct: 305 KGEYIQRFDSYSFFHNKCLCGAPLPSCK 330

BLAST of Tan0020796 vs. TAIR 10
Match: AT3G12145.1 (Leucine-rich repeat (LRR) family protein )

HSP 1 Score: 313.2 bits (801), Expect = 2.6e-85
Identity = 165/323 (51.08%), Postives = 216/323 (66.87%), Query Frame = 0

Query: 14  LLVFF--IFITISFAELCNPNDKKALLNIKKAFNNPYILTSWDPETDCC-HWYCVKCNRT 73
           L +FF  +FIT+  +  C  NDK ALL IKKA  NP +L+SW+P TDCC  W  V+C  T
Sbjct: 7   LSIFFSILFITLPSSYSCTENDKNALLQIKKALGNPPLLSSWNPRTDCCTGWTGVEC--T 66

Query: 74  THRITSLTIFADDRLSGQIPPEVGDLPFLETLMLHKLPNLTGPIQPTTAKLHNLKILDLS 133
             R+T L++ + + +SGQI  ++GDL  L TL    LP+LTG I  T  KL NL  L L 
Sbjct: 67  NRRVTGLSVTSGE-VSGQISYQIGDLVDLRTLDFSYLPHLTGNIPRTITKLKNLNTLYLK 126

Query: 134 WNSLSGPIPDFLSTLTNLFVLSLSFNNLIGTIPSSLSKLPNLGGLQLDRNKLTGQIPDSF 193
             SLSGPIPD++S L +L  L LSFN   G IP SLS++P L  +Q++ NKLTG IP+SF
Sbjct: 127 HTSLSGPIPDYISELKSLTFLDLSFNQFTGPIPGSLSQMPKLEAIQINDNKLTGSIPNSF 186

Query: 194 GYFPGEQAPSLYLSHNQLSGKIPSSLSRLNLTSIDFSRNKLVGDAFMIFGSNKTVQIVDL 253
           G F G   P+LYLS+N+LSGKIP SLS+ +  ++D S N   GDAFM FG NKT   VDL
Sbjct: 187 GSFVG-NVPNLYLSNNKLSGKIPESLSKYDFNAVDLSGNGFEGDAFMFFGRNKTTVRVDL 246

Query: 254 SRNLLEFNLSEVVFPQSLTSLDLNHNKVFGGLSPEMMKLNFQYLNVSYNRLCGEIPVGGE 313
           SRN+  F+L +V F +S+ SLDL+ N ++G + P + KL+ ++ NVS N LCG+IP GG 
Sbjct: 247 SRNMFNFDLVKVKFARSIVSLDLSQNHIYGKIPPALTKLHLEHFNVSDNHLCGKIPSGGL 306

Query: 314 LQKFDVYSYFHNKCLCGAPLGSC 334
           LQ F+  ++ HN CLCG PL +C
Sbjct: 307 LQTFEPSAFAHNICLCGTPLKAC 325

BLAST of Tan0020796 vs. TAIR 10
Match: AT3G12610.1 (Leucine-rich repeat (LRR) family protein )

HSP 1 Score: 184.5 bits (467), Expect = 1.4e-46
Identity = 122/368 (33.15%), Postives = 185/368 (50.27%), Query Frame = 0

Query: 6   KLPWLPLFLLVFFIFIT-ISFAELCNPNDKKALLNIKKAFNNPY--ILTSWDPETDCC-H 65
           KL   P   L+  +FI+ IS    C+P D+ AL   K + + P   I  +W   TDCC  
Sbjct: 3   KLLASPFSSLLAVVFISVISVVRCCSPKDQTALNAFKSSLSEPNLGIFNTWSENTDCCKE 62

Query: 66  WYCVKCNRTTHRITSLTIFADDR------------LSGQIPPEVGDLPFLETLMLHKLPN 125
           WY + C+  + R+T +++  +              +SG I P V DL  L +L+L     
Sbjct: 63  WYGISCDPDSGRVTDISLRGESEDAIFQKAGRSGYMSGSIDPAVCDLTALTSLVLADWKG 122

Query: 126 LTGPIQPTTAKLHNLKILDLSWNSLSGPIPDFLSTLTNLFVLSLSFNNLIGTIPSSLSKL 185
           +TG I P    L +L+ILDL+ N ++G IP  +  L+ L VL+L+ N + G IP+SL+ L
Sbjct: 123 ITGEIPPCITSLASLRILDLAGNKITGEIPAEIGKLSKLAVLNLAENQMSGEIPASLTSL 182

Query: 186 PNLGGLQLDRNKLTGQIPDSFGYFPGEQAPSLYLSHNQLSGKIPSSLSRL-NLTSIDFSR 245
             L  L+L  N +TG IP  FG    +    + L  N+L+G IP S+S +  L  +D S+
Sbjct: 183 IELKHLELTENGITGVIPADFGSL--KMLSRVLLGRNELTGSIPESISGMERLADLDLSK 242

Query: 246 NKLVG-----------------DAFMIFG-------SNKTVQIVDLSRNLLEFNLSEVVF 305
           N + G                 D   + G       SN  + + +LSRN LE  + +V  
Sbjct: 243 NHIEGPIPEWMGNMKVLSLLNLDCNSLTGPIPGSLLSNSGLDVANLSRNALEGTIPDVFG 302

Query: 306 PQS-LTSLDLNHNKVFGGLSPEMMKLNF-QYLNVSYNRLCGEIPVGGELQKFDVYSYFHN 331
            ++ L SLDL+HN + G +   +    F  +L++S+N+LCG IP G      +  S+  N
Sbjct: 303 SKTYLVSLDLSHNSLSGRIPDSLSSAKFVGHLDISHNKLCGRIPTGFPFDHLEATSFSDN 362

BLAST of Tan0020796 vs. TAIR 10
Match: AT3G20820.1 (Leucine-rich repeat (LRR) family protein )

HSP 1 Score: 174.1 bits (440), Expect = 1.9e-43
Identity = 118/365 (32.33%), Postives = 184/365 (50.41%), Query Frame = 0

Query: 12  LFLLVFFIFITISFAELCNPNDKKALLNIKKAFNNPY--ILTSWDPETDCCH-WYCVKCN 71
           L ++V  + + IS A  C P+D++ALL  + A + PY  I  SW  + DCCH WY + C+
Sbjct: 3   LNVVVLLLLLLISSATCCPPSDRRALLAFRSALHEPYLGIFNSWTGQ-DCCHNWYGISCD 62

Query: 72  RTTHRITSLTIFADDR-------------------------------------LSGQIPP 131
             THR+  + +  +                                       +SG+IP 
Sbjct: 63  SLTHRVADINLRGESEDPIFERAHRTGYMTGHISASICELTRLSAITIADWKGISGEIPK 122

Query: 132 EVGDLPFLETLMLHKLPNLTGPIQPTTAKLHNLKILDLSWNSLSGPIPDFLSTLTNLFVL 191
            +  LPFL TL L     ++G I     +L+ L +L+++ N +SG IP  L+ L++L  L
Sbjct: 123 CITRLPFLRTLDLIG-NQISGGIPYDIGRLNRLAVLNVADNRISGSIPKSLTNLSSLMHL 182

Query: 192 SLSFNNLIGTIPSSLSKLPNLGGLQLDRNKLTGQIPDSFGYFPGEQAPSLYLSHNQLSGK 251
            L  N + G IPS + +L  L    L  N++TG+IP+S       +   + LS NQL G 
Sbjct: 183 DLRNNLISGVIPSDVGRLKMLSRALLSGNRITGRIPESLTNI--YRLADVDLSGNQLYGT 242

Query: 252 IPSSLSRLN-LTSIDFSRNKLVGDAFMIFGSNKTVQIVDLSRNLLEFNLSEVVFPQS-LT 311
           IP SL R++ L +++   NK+ G+         +V  ++LSRNLL+  + E   P+S  T
Sbjct: 243 IPPSLGRMSVLATLNLDGNKISGEIPQTL-MTSSVMNLNLSRNLLQGKIPEGFGPRSYFT 302

Query: 312 SLDLNHNKVFGGLSPEMMKLNF-QYLNVSYNRLCGEIPVGGELQKFDVYSYFHNKCLCGA 334
            LDL++N + G +   +   +F  +L++S+N LCG IPVG      +  S+  N CLCG 
Sbjct: 303 VLDLSYNNLKGPIPRSISGASFIGHLDLSHNHLCGRIPVGSPFDHLEAASFMFNDCLCGK 362

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q050911.7e-12669.06Polygalacturonase inhibitor OS=Pyrus communis OX=23211 GN=PGIP PE=1 SV=1[more]
A7PW814.0e-11565.92Polygalacturonase inhibitor OS=Vitis vinifera OX=29760 GN=pgip PE=1 SV=1[more]
Q9M5J92.1e-11161.04Polygalacturonase inhibitor 1 OS=Arabidopsis thaliana OX=3702 GN=PGIP1 PE=1 SV=1[more]
Q9M5J86.9e-10759.45Polygalacturonase inhibitor 2 OS=Arabidopsis thaliana OX=3702 GN=PGIP2 PE=2 SV=2[more]
Q9LH523.7e-8451.08Leucine-rich repeat protein FLOR 1 OS=Arabidopsis thaliana OX=3702 GN=FLR1 PE=1 ... [more]
Match NameE-valueIdentityDescription
XP_023517351.15.5e-17088.92polygalacturonase inhibitor-like [Cucurbita pepo subsp. pepo][more]
KAG6595658.17.2e-17088.32Polygalacturonase inhibitor, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022966389.16.1e-16988.62polygalacturonase inhibitor-like [Cucurbita maxima][more]
XP_022925134.11.8e-16888.29polygalacturonase inhibitor-like [Cucurbita moschata][more]
KAA0044799.12.6e-16485.07polygalacturonase inhibitor-like [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
A0A6J1HTN42.9e-16988.62polygalacturonase inhibitor-like OS=Cucurbita maxima OX=3661 GN=LOC111466052 PE=... [more]
A0A6J1EB928.6e-16988.29polygalacturonase inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111432468 P... [more]
A0A5D3CYT31.3e-16484.57Polygalacturonase inhibitor-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A5A7TU821.3e-16485.07Polygalacturonase inhibitor-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27... [more]
A0A1S3BU591.3e-16484.57polygalacturonase inhibitor-like OS=Cucumis melo OX=3656 GN=LOC103493193 PE=4 SV... [more]
Match NameE-valueIdentityDescription
AT5G06860.11.5e-11261.04polygalacturonase inhibiting protein 1 [more]
AT5G06870.14.9e-10859.45polygalacturonase inhibiting protein 2 [more]
AT3G12145.12.6e-8551.08Leucine-rich repeat (LRR) family protein [more]
AT3G12610.11.4e-4633.15Leucine-rich repeat (LRR) family protein [more]
AT3G20820.11.9e-4332.33Leucine-rich repeat (LRR) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR032675Leucine-rich repeat domain superfamilyGENE3D3.80.10.10Ribonuclease Inhibitorcoord: 27..334
e-value: 3.3E-81
score: 274.8
IPR013210Leucine-rich repeat-containing N-terminal, plant-typePFAMPF08263LRRNT_2coord: 31..68
e-value: 1.7E-11
score: 44.2
IPR001611Leucine-rich repeatPFAMPF13516LRR_6coord: 266..278
e-value: 0.89
score: 9.8
IPR001611Leucine-rich repeatPFAMPF00560LRR_1coord: 123..143
e-value: 0.02
score: 15.4
coord: 200..219
e-value: 1.1
score: 10.1
NoneNo IPR availablePANTHERPTHR48059:SF10POLYGALACTURONASE INHIBITOR 2coord: 10..333
NoneNo IPR availablePANTHERPTHR48059POLYGALACTURONASE INHIBITOR 1coord: 10..333
NoneNo IPR availableSUPERFAMILY52058L domain-likecoord: 28..332

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0020796.1Tan0020796.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0043086 negative regulation of catalytic activity
molecular_function GO:0090353 polygalacturonase inhibitor activity
molecular_function GO:0005515 protein binding