Tan0020634 (gene) Snake gourd v1

Overview
NameTan0020634
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptiontRNA-intron lyase
LocationLG05: 2166909 .. 2169690 (+)
RNA-Seq ExpressionTan0020634
SyntenyTan0020634
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GGAAATTAGTATCTCCAATCTTCATCTTCTTCGCAACACTCCCCCTTCGTCTCTGTGTTCGCTGGTCTTCTTCCTTCTTTAGGTTTAAGGTATTCTCTCAATCTCCAATTTTGGCTTCATTTCCTCCTCAAAACCGACGAAAGTTGAAACCCCCCTCTAGCTTCTCTCTCCTAAACCGATCAAATCGAACCAAATCAATTCTTGTTAGTCGACATTCGAAGGCACACATTTAAACCCTCGATTTTACGGTTTCAACTCCTTCAGGTCGGCTTTAATGGGGCCAAGATGGAAAGGGAAGGGTTCAGAAGCCAAAGCTTTAGCAGATCCCATGTCGAAGATTGTACTTCAGCTTCAATCTTCTCTAACACAGTCGGACGCTCAAGGATTATTATCTAGTTCTAGTGTACTTTATCAGGCGAATGAAGAACAAGCCGAACTTCTTAATCGCGCTTGTTTCGGTCGACCAATTGTTTCCGCCGAGAAGGATAAGCAGTGGTTTCAATTGGGCATGGAGGAGGCTTTTTACTTGTCCTATCATTTGAATTGCCTTAAAATTGTCGACGGACAGGATTGTGAAAAGAATTTCGAAGAACTCTGGCAATATATGAAGTCCCAAAAGGCTACATTCCCTGAGTTTTACAAGGCTTATCAAAATCTTCGGATGAAGAATTGGGTGGTGAGACCTGGGTCTCAATATGGGGTTGATTTTGTGGCTTACCGTCACCATCCGGCTCTCGTCCACTCTGAATTTGCGGTGCTTGTGTTGTCGGAAGGAGGAGGCAGTAATGAAAATGGGCGATTGAGAGTTTGGTCTGATTTTCTTTGCACAATTCGGCTTTGTGGAAGTGTTGCAAAAACGCTGCTAGTTCTCACCGTCAATGATAATGGAAGCGGTTCTGTTTCTCCTTCGTGTTTGGACAGTTACTCCATCGAAGAGCGCACAGTTACAAGATGGAGTCCAGAACAGTGCCGCGAAAATCCTGCAAAAGTGTGAAAAATATGCCGAGTAAGATCCGATCTTTATTTGTTATTTGGATAATTTCTTATTGTTCTTCACTTACAATGAAAGTGGACTAGTCAGAGCTACATAACTGGTTTAGTTTCAGATGGGTTTTGAAGATGATATAGTCACAATCATGTTCTTCCAATTAATTCCAACTTGAAATTGCTCAAATACTCTTTCAGGAATGACTACAATTACACAACTAAACAATGTTGAATGTAAAAATCCAAAACCTGAAAATACATACACAGATATCATATACTTCAGTAAAAGATTGGTTGATGTTAAACTACACCAAATCCAAAGAGCAAACCTTAGCATAAGATCTGAGAAATGACTATCAATAGTAGGAGTTTGAAGGTTGAGTTATTGCTAAACTCAAAAGTTTATGTTGATTAGGTAGAGTATATGTAATCTTTTATTTATATTCTTAACATTTGAATAAGTATCTAGTTGGGTTGTGAGTTCTCCATCCGTACTTTCCACCGGGAGAATTCATCTTTATTGATCATTAGTTTTTCCTTAATGTCCCAATGGGAATGTCATAAACAAGCAATTGAATGATTAAGACAATGTTGCTTGCCAGAGACATGTTCCTTCTGCTCTTCTGGGACCCTCCATGCAAGAAATGTAGACATAGTAGAGTTCTCCTGAAAATCGCTCCACTTTGGATTGGCTAACTGGCCAGTTCAAAATGTCTCATTATGTAGACATAGCCTCTCTTACATTCTCTGCATCAAAGACTGAACAAACCAGATTGAAAATATTAGAATGCAGCAAGCAATTATCTTCTGAACATAATAAAGCCATTACTCAAAGTTCGACTTGCAAGTATCATTGTTCTTTTTTGCATACAAACATACGTTGTACATAATTGAATGACTAGAAAAGTGGGTGTACTGAAAATATCATGATCTTGTAAGTATCATATAATTGAATGAAAAAGGGAGCATCCCTTTTTCTGAAATAGGCCTCTTACAGTCTAATGTTTGTATGCAGTCAAGTTAACCTCTTACAAGATGAAGAATTGTGTGGGGAAATGATATTGTCTGTTTTAATGGAGGCTGCCCCAGATATGCTATATTTCCATGGAAGGAAGACCGGGTGAAGGACTAGGATGATCATCTCCCCTGTTTCATTATCAATCCTTATCTGCAATCTTTCTTGGCTATTGAATGAAGCTAGTCAGTTTATTGGTTTTTATTTATCAGGCAAAATGTCATTTTTTTCTTAGACACAACAGCTTGCAAAATTGGCGTCTGAATCTTATAGTTCTGCAATGCATTGAGGATTTGATGGAAGAGTGCAGAAATAAACAGGACAATCACAGTTATGATGGGCTGTTACGACTGCACGACGATCATCCTACAGCTGTTGTGCAGGAGGGATGAGGAAGCAAAGCGTTGGAATATCTTTAGCTAGAGGAGGTGAAGGAAACTTTCATTCAGTGCACATTGTAGAGTTGCTGAGAAGTGAGTTTCTTTGTAGCCTTGACTACCAAAGTTTCAACACCCAAAAGACAAAATTCAAAGGAAAAATCCACATATCTCCCTCCCTCAAGCTTGTATGAGCCAGAAAAAAAACCATAAATTGCCTCTGTGGCTGTTGTTTAGCTGCGGATAGTTTATCCCATTTGAACTACAGTTTGGAAGGGATGGTTTGATGATTGCTTGAAGAATTAAACATGGGGTTTTCGATGATCCTCTTTTGTTGCTAGAACTATCTTTTGGTTGATTTAAGAGGATGTAAGAGGATTTTATTTGTGCTGTCCTTTGCGGC

mRNA sequence

GGAAATTAGTATCTCCAATCTTCATCTTCTTCGCAACACTCCCCCTTCGTCTCTGTGTTCGCTGGTCTTCTTCCTTCTTTAGGTTTAAGGTATTCTCTCAATCTCCAATTTTGGCTTCATTTCCTCCTCAAAACCGACGAAAGTTGAAACCCCCCTCTAGCTTCTCTCTCCTAAACCGATCAAATCGAACCAAATCAATTCTTGTTAGTCGACATTCGAAGGCACACATTTAAACCCTCGATTTTACGGTTTCAACTCCTTCAGGTCGGCTTTAATGGGGCCAAGATGGAAAGGGAAGGGTTCAGAAGCCAAAGCTTTAGCAGATCCCATGTCGAAGATTGTACTTCAGCTTCAATCTTCTCTAACACAGTCGGACGCTCAAGGATTATTATCTAGTTCTAGTGTACTTTATCAGGCGAATGAAGAACAAGCCGAACTTCTTAATCGCGCTTGTTTCGGTCGACCAATTGTTTCCGCCGAGAAGGATAAGCAGTGGTTTCAATTGGGCATGGAGGAGGCTTTTTACTTGTCCTATCATTTGAATTGCCTTAAAATTGTCGACGGACAGGATTGTGAAAAGAATTTCGAAGAACTCTGGCAATATATGAAGTCCCAAAAGGCTACATTCCCTGAGTTTTACAAGGCTTATCAAAATCTTCGGATGAAGAATTGGGTGGTGAGACCTGGGTCTCAATATGGGGTTGATTTTGTGGCTTACCGTCACCATCCGGCTCTCGTCCACTCTGAATTTGCGGTGCTTGTGTTGTCGGAAGGAGGAGGCAGTAATGAAAATGGGCGATTGAGAGTTTGGTCTGATTTTCTTTGCACAATTCGGCTTTGTGGAAGTGTTGCAAAAACGCTGCTAGTTCTCACCGTCAATGATAATGGAAGCGGTTCTGTTTCTCCTTCGTGTTTGGACAGTTACTCCATCGAAGAGCGCACAGTTACAAGATGGAGTCCAGAACAGTGCCGCGAAAATCCTGCAAAAGTGTGAAAAATATGCCGATCAAGTTAACCTCTTACAAGATGAAGAATTGTGTGGGGAAATGATATTGTCTGTTTTAATGGAGGCTGCCCCAGATATGCTATATTTCCATGGAAGGAAGACCGGGTGAAGGACTAGGATGATCATCTCCCCTGTTTCATTATCAATCCTTATCTGCAATCTTTCTTGGCTATTGAATGAAGCTAGTCAGTTTATTGGTTTTTATTTATCAGGCAAAATGTCATTTTTTTCTTAGACACAACAGCTTGCAAAATTGGCGTCTGAATCTTATAGTTCTGCAATGCATTGAGGATTTGATGGAAGAGTGCAGAAATAAACAGGACAATCACAGTTATGATGGGCTGTTACGACTGCACGACGATCATCCTACAGCTGTTGTGCAGGAGGGATGAGGAAGCAAAGCGTTGGAATATCTTTAGCTAGAGGAGGTGAAGGAAACTTTCATTCAGTGCACATTGTAGAGTTGCTGAGAAGTGAGTTTCTTTGTAGCCTTGACTACCAAAGTTTCAACACCCAAAAGACAAAATTCAAAGGAAAAATCCACATATCTCCCTCCCTCAAGCTTGTATGAGCCAGAAAAAAAACCATAAATTGCCTCTGTGGCTGTTGTTTAGCTGCGGATAGTTTATCCCATTTGAACTACAGTTTGGAAGGGATGGTTTGATGATTGCTTGAAGAATTAAACATGGGGTTTTCGATGATCCTCTTTTGTTGCTAGAACTATCTTTTGGTTGATTTAAGAGGATGTAAGAGGATTTTATTTGTGCTGTCCTTTGCGGC

Coding sequence (CDS)

ATGGGGCCAAGATGGAAAGGGAAGGGTTCAGAAGCCAAAGCTTTAGCAGATCCCATGTCGAAGATTGTACTTCAGCTTCAATCTTCTCTAACACAGTCGGACGCTCAAGGATTATTATCTAGTTCTAGTGTACTTTATCAGGCGAATGAAGAACAAGCCGAACTTCTTAATCGCGCTTGTTTCGGTCGACCAATTGTTTCCGCCGAGAAGGATAAGCAGTGGTTTCAATTGGGCATGGAGGAGGCTTTTTACTTGTCCTATCATTTGAATTGCCTTAAAATTGTCGACGGACAGGATTGTGAAAAGAATTTCGAAGAACTCTGGCAATATATGAAGTCCCAAAAGGCTACATTCCCTGAGTTTTACAAGGCTTATCAAAATCTTCGGATGAAGAATTGGGTGGTGAGACCTGGGTCTCAATATGGGGTTGATTTTGTGGCTTACCGTCACCATCCGGCTCTCGTCCACTCTGAATTTGCGGTGCTTGTGTTGTCGGAAGGAGGAGGCAGTAATGAAAATGGGCGATTGAGAGTTTGGTCTGATTTTCTTTGCACAATTCGGCTTTGTGGAAGTGTTGCAAAAACGCTGCTAGTTCTCACCGTCAATGATAATGGAAGCGGTTCTGTTTCTCCTTCGTGTTTGGACAGTTACTCCATCGAAGAGCGCACAGTTACAAGATGGAGTCCAGAACAGTGCCGCGAAAATCCTGCAAAAGTGTGA

Protein sequence

MGPRWKGKGSEAKALADPMSKIVLQLQSSLTQSDAQGLLSSSSVLYQANEEQAELLNRACFGRPIVSAEKDKQWFQLGMEEAFYLSYHLNCLKIVDGQDCEKNFEELWQYMKSQKATFPEFYKAYQNLRMKNWVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEGGGSNENGRLRVWSDFLCTIRLCGSVAKTLLVLTVNDNGSGSVSPSCLDSYSIEERTVTRWSPEQCRENPAKV
Homology
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BLAST of Tan0020634 vs. ExPASy Swiss-Prot
Match: Q9M1E8 (tRNA-splicing endonuclease subunit Sen2-1 OS=Arabidopsis thaliana OX=3702 GN=SEN1 PE=2 SV=1)

HSP 1 Score: 268.1 bits (684), Expect = 9.7e-71
Identity = 140/241 (58.09%), Postives = 173/241 (71.78%), Query Frame = 0 

Query: 1   MGPRWKGKGSEAKALADPMSKIVLQLQSSLTQSDAQGLLSSSSVLYQANEEQAELLNRAC 60
           M PRWK KG+EAKALA+P+SK V +L+SSLTQ++A G LSS +VL     E+AELL+R C
Sbjct: 1   MAPRWKWKGAEAKALAEPVSKTVSELRSSLTQTEALGFLSSCNVLLSVESEEAELLDRCC 60

Query: 61  FGRPIVSAEKDKQWFQLGMEEAFYLSYHLNCLKI-VDGQDCEKNFEELWQYMKSQKATFP 120
           FGR +V AEKDK+W QL  EEAF+L Y L C+KI + G+  E N  +LW+ M S K  F 
Sbjct: 61  FGRLVVGAEKDKRWIQLSFEEAFFLFYKLKCIKICLHGRSLE-NEVDLWRSMSSFKQDFA 120

Query: 121 EFYKAYQNLRMKNWVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEGGGSNENGRLRVW 180
             YKAY +LR KNW+VR G QYGVDFV YRHHP+LVHSE+AVLV S GG    N RL+VW
Sbjct: 121 ILYKAYSHLRSKNWIVRSGLQYGVDFVVYRHHPSLVHSEYAVLVQSIGG----NDRLKVW 180

Query: 181 SDFLCTIRLCGSVAKTLLVLTVND--NGSGSVSPSCLDSYSIEERTVTRWSPEQCRENPA 239
           SD  C++RL GSVAK+LLVL VN   N      P CL+ Y++EE+T+ RWSPE  RE+  
Sbjct: 181 SDIHCSVRLTGSVAKSLLVLYVNRKVNTEKMNLPLCLEDYTVEEQTIRRWSPELSREDET 236

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BLAST of Tan0020634 vs. ExPASy Swiss-Prot
Match: Q9LSS3 (tRNA-splicing endonuclease subunit Sen2-2 OS=Arabidopsis thaliana OX=3702 GN=SEN2 PE=2 SV=1)

HSP 1 Score: 266.9 bits (681), Expect = 2.2e-70
Identity = 134/237 (56.54%), Postives = 172/237 (72.57%), Query Frame = 0 

Query: 1   MGPRWKGKGSEAKALADPMSKIVLQLQSSLTQSDAQGLLSSSSVLYQANEEQAELLNRAC 60
           M PRWK KG+EAKALA+P+SK V +LQ SL ++++ G LSS +VL     EQAELL+R C
Sbjct: 1   MAPRWKWKGAEAKALAEPISKSVSELQLSLAETESSGTLSSCNVLLAVEPEQAELLDRCC 60

Query: 61  FGRPIVSAEKDKQWFQLGMEEAFYLSYHLNCLKIVDGQDCEKNFEELWQYMKSQKATFPE 120
           FGR ++SAEK K+W QL  EEAF+L Y+L C+KI     C +N  + W YMKS++  FP 
Sbjct: 61  FGRLVLSAEKIKKWIQLSFEEAFFLHYNLKCIKISLQGRCLENEVDTWLYMKSKRPNFPM 120

Query: 121 FYKAYQNLRMKNWVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEGGGSNENGRLRVWS 180
           F+KAY +LR KNWV+R G QYGVDFVAYRHHP+LVHSE++VLV      S ++ RLRVWS
Sbjct: 121 FFKAYSHLRSKNWVLRSGLQYGVDFVAYRHHPSLVHSEYSVLV-----QSGDSDRLRVWS 180

Query: 181 DFLCTIRLCGSVAKTLLVLTVNDNGSGSVSP--SCLDSYSIEERTVTRWSPEQCREN 236
           D  C +RL GSVAKTLL L VN N  G       CL+++++EE+T++RWSPE  RE+
Sbjct: 181 DIHCAVRLSGSVAKTLLTLYVNGNFKGEDVNLLVCLENFTVEEQTISRWSPELSRED 232

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BLAST of Tan0020634 vs. ExPASy Swiss-Prot
Match: Q5Z6B1 (Probable tRNA-splicing endonuclease subunit Sen2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g0530700 PE=2 SV=1)

HSP 1 Score: 238.0 bits (606), Expect = 1.1e-61
Identity = 125/241 (51.87%), Postives = 156/241 (64.73%), Query Frame = 0 

Query: 2   GPRWK----GKGSEAKALADPMSKIVLQLQSSLTQSDAQGLLS--SSSVLYQANEEQAEL 61
           GPRWK    GK   + A A+PMS IV +L++S   S    +LS    S +     EQA +
Sbjct: 5   GPRWKKGKDGKDFASLAAANPMSAIVSELKASFISSKPVAILSGPGGSAVLGVGPEQAVI 64

Query: 62  LNRACFGRPIVSAEKDKQWFQLGMEEAFYLSYHLNCLKIVDGQDCEKNFEELWQYMKSQK 121
           LNRA FG  I +A   K WFQL  EE FYL + LNC+++    + + +  ELW Y +S  
Sbjct: 65  LNRAAFGHAIENATAQKHWFQLSPEEVFYLCHALNCIRVDSLDNKQMSEIELWDYFRSGS 124

Query: 122 ATFPEFYKAYQNLRMKNWVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEGGG-SNENG 181
            +FPE YKAY +LR+KNWVVR G QYG DFVAYRHHPALVHSEFAV+V+ EG    N  G
Sbjct: 125 ESFPEMYKAYAHLRLKNWVVRSGLQYGADFVAYRHHPALVHSEFAVVVVPEGAEFGNRCG 184

Query: 182 RLRVWSDFLCTIRLCGSVAKTLLVLTVNDNGSGSV-SPSCLDSYSIEERTVTRWSPEQCR 235
           RL VWSD LC +R  GSVAKTLLVLT++ +    + SP CL+   + ERT+TRW  +QCR
Sbjct: 185 RLEVWSDLLCALRASGSVAKTLLVLTISSSSKCELSSPDCLEQLVVHERTITRWILQQCR 244

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BLAST of Tan0020634 vs. ExPASy Swiss-Prot
Match: Q9P6Y2 (Probable tRNA-splicing endonuclease subunit tsp-2 OS=Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) OX=367110 GN=tsp-2 PE=3 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 1.2e-09
Identity = 51/192 (26.56%), Postives = 90/192 (46.88%), Query Frame = 0 

Query: 71  DKQWFQLGMEEAFYLSYHLNCLKIVDG-QDCEKNFEELWQYMK-------------SQKA 130
           DK+ FQL  EEAF+L++ L  L++VD   +   + E+L   ++             S + 
Sbjct: 444 DKEHFQLAPEEAFFLTFGLGALRVVDPVTEAPISNEQLLIKLRANSYFPPRSVDNLSPED 503

Query: 131 TFPEFYKAYQNLRMKNWVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEGGG------S 190
            F   Y  Y + R   WV R G ++GVD++ Y+  P   HSEF ++V+            
Sbjct: 504 PFLVQYAVYHHFRSLGWVPRHGIKFGVDWIIYQRGPVFDHSEFGIMVVPSFSDPRWSEFE 563

Query: 191 NENGRLRVWSDFLCTIRLCGSVAKTLLVLTVN---------DNGSGSVSPSCLDSYSIEE 234
           +E  + + WS  +   R+   V K+L+++ V+         +   G ++ + L  ++I E
Sbjct: 564 HEESK-KTWSWLMGVNRVLSHVLKSLVLVYVDVPPPPVFDEEMKKGGIA-AALKKFTIRE 623

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BLAST of Tan0020634 vs. ExPASy Swiss-Prot
Match: Q97ZY3 (tRNA-splicing endonuclease OS=Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) OX=273057 GN=endA PE=3 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 4.7e-09
Identity = 39/134 (29.10%), Postives = 68/134 (50.75%), Query Frame = 0 

Query: 36  QGLLSSSSVLYQANEEQAELLNRACFGRPI-VSAEKDK----QWFQLGMEEAFYLSYHLN 95
           + LL  S VL  + +E   L +   +G+PI +S  K      +  +L + E+ YL+    
Sbjct: 3   KALLVGSKVLVPSIDESRYLYSNGFYGKPIGISKPKGPKDIVRPLELSLIESVYLT-KKG 62

Query: 96  CLKIVDGQDCEKNFEELWQYMKSQKATFPEFYKAYQNLRMKNWVVRPGSQYGVDFVAYRH 155
            + +VD       +++L++Y   +   F   YK Y++LR K ++VR G +YG DF  Y  
Sbjct: 63  LINVVDKNGDLLEYKKLYEYSAMKINKFEILYKVYEDLREKGFIVRSGVKYGADFAVYTL 122

Query: 156 HPALVHSEFAVLVL 165
            P L H+ + V+ +
Sbjct: 123 GPGLEHAPYVVIAV 135

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BLAST of Tan0020634 vs. NCBI nr
Match: XP_023532286.1 (tRNA-splicing endonuclease subunit Sen2-1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 469.5 bits (1207), Expect = 1.7e-128
Identity = 228/237 (96.20%), Postives = 231/237 (97.47%), Query Frame = 0 

Query: 1   MGPRWKGKGSEAKALADPMSKIVLQLQSSLTQSDAQGLLSSSSVLYQANEEQAELLNRAC 60
           MGPRWKGKGSEAKALADPMSKIVLQLQSSLTQSDAQGLLSS SVLYQA+EEQAELLNRAC
Sbjct: 1   MGPRWKGKGSEAKALADPMSKIVLQLQSSLTQSDAQGLLSSCSVLYQADEEQAELLNRAC 60

Query: 61  FGRPIVSAEKDKQWFQLGMEEAFYLSYHLNCLKIVDGQDCEKNFEELWQYMKSQKATFPE 120
           FGRPIVSAEKDKQWFQ GMEEAFYLSYHLNCLKIVDGQDCEKNF+ELW YMKSQKATFPE
Sbjct: 61  FGRPIVSAEKDKQWFQFGMEEAFYLSYHLNCLKIVDGQDCEKNFDELWHYMKSQKATFPE 120

Query: 121 FYKAYQNLRMKNWVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEGGGSNENGRLRVWS 180
           FYKAYQNLRMKNWVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEG GSNENGRLRVWS
Sbjct: 121 FYKAYQNLRMKNWVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEGEGSNENGRLRVWS 180

Query: 181 DFLCTIRLCGSVAKTLLVLTVNDNGSGSVSPSCLDSYSIEERTVTRWSPEQCRENPA 238
           DFLCTIRLCGSVAKTLLVLTVN NG+ SVSPSCLDSYSIEERTVTRWSPEQCRENPA
Sbjct: 181 DFLCTIRLCGSVAKTLLVLTVNSNGNRSVSPSCLDSYSIEERTVTRWSPEQCRENPA 237

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BLAST of Tan0020634 vs. NCBI nr
Match: XP_022947593.1 (tRNA-splicing endonuclease subunit Sen2-1-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 464.9 bits (1195), Expect = 4.1e-127
Identity = 226/237 (95.36%), Postives = 231/237 (97.47%), Query Frame = 0 

Query: 1   MGPRWKGKGSEAKALADPMSKIVLQLQSSLTQSDAQGLLSSSSVLYQANEEQAELLNRAC 60
           MGPRWKGKGSEAKALADP+SKIVLQLQSSLTQSDAQGLLSS SVLYQA+EEQAELLNRAC
Sbjct: 1   MGPRWKGKGSEAKALADPVSKIVLQLQSSLTQSDAQGLLSSCSVLYQADEEQAELLNRAC 60

Query: 61  FGRPIVSAEKDKQWFQLGMEEAFYLSYHLNCLKIVDGQDCEKNFEELWQYMKSQKATFPE 120
           FGRPIVSAEKDKQWFQ GMEEAFYLSY+LNCLKIVDGQDCEKNF+ELW YMKSQKATFPE
Sbjct: 61  FGRPIVSAEKDKQWFQFGMEEAFYLSYYLNCLKIVDGQDCEKNFDELWLYMKSQKATFPE 120

Query: 121 FYKAYQNLRMKNWVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEGGGSNENGRLRVWS 180
           FYKAYQNLRMKNWVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEG GSNENGRLRVWS
Sbjct: 121 FYKAYQNLRMKNWVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEGEGSNENGRLRVWS 180

Query: 181 DFLCTIRLCGSVAKTLLVLTVNDNGSGSVSPSCLDSYSIEERTVTRWSPEQCRENPA 238
           DFLCTIRLCGSVAKTLLVLTVN NG+ SVSPSCLDSYSIEERTVTRWSPEQCRENPA
Sbjct: 181 DFLCTIRLCGSVAKTLLVLTVNSNGNRSVSPSCLDSYSIEERTVTRWSPEQCRENPA 237

[Close]
BLAST of Tan0020634 vs. NCBI nr
Match: XP_022947592.1 (tRNA-splicing endonuclease subunit Sen2-1-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 464.9 bits (1195), Expect = 4.1e-127
Identity = 226/237 (95.36%), Postives = 231/237 (97.47%), Query Frame = 0 

Query: 1   MGPRWKGKGSEAKALADPMSKIVLQLQSSLTQSDAQGLLSSSSVLYQANEEQAELLNRAC 60
           MGPRWKGKGSEAKALADP+SKIVLQLQSSLTQSDAQGLLSS SVLYQA+EEQAELLNRAC
Sbjct: 1   MGPRWKGKGSEAKALADPVSKIVLQLQSSLTQSDAQGLLSSCSVLYQADEEQAELLNRAC 60

Query: 61  FGRPIVSAEKDKQWFQLGMEEAFYLSYHLNCLKIVDGQDCEKNFEELWQYMKSQKATFPE 120
           FGRPIVSAEKDKQWFQ GMEEAFYLSY+LNCLKIVDGQDCEKNF+ELW YMKSQKATFPE
Sbjct: 61  FGRPIVSAEKDKQWFQFGMEEAFYLSYYLNCLKIVDGQDCEKNFDELWLYMKSQKATFPE 120

Query: 121 FYKAYQNLRMKNWVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEGGGSNENGRLRVWS 180
           FYKAYQNLRMKNWVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEG GSNENGRLRVWS
Sbjct: 121 FYKAYQNLRMKNWVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEGEGSNENGRLRVWS 180

Query: 181 DFLCTIRLCGSVAKTLLVLTVNDNGSGSVSPSCLDSYSIEERTVTRWSPEQCRENPA 238
           DFLCTIRLCGSVAKTLLVLTVN NG+ SVSPSCLDSYSIEERTVTRWSPEQCRENPA
Sbjct: 181 DFLCTIRLCGSVAKTLLVLTVNSNGNRSVSPSCLDSYSIEERTVTRWSPEQCRENPA 237

[Close]
BLAST of Tan0020634 vs. NCBI nr
Match: KAG6605517.1 (tRNA-splicing endonuclease subunit Sen2-2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 462.6 bits (1189), Expect = 2.0e-126
Identity = 226/237 (95.36%), Postives = 229/237 (96.62%), Query Frame = 0 

Query: 1   MGPRWKGKGSEAKALADPMSKIVLQLQSSLTQSDAQGLLSSSSVLYQANEEQAELLNRAC 60
           MG RWKGKGSEAKALADPMSKIVLQLQSSLTQSDAQGLLSS SVLYQA+EEQAELLNRAC
Sbjct: 1   MGLRWKGKGSEAKALADPMSKIVLQLQSSLTQSDAQGLLSSCSVLYQADEEQAELLNRAC 60

Query: 61  FGRPIVSAEKDKQWFQLGMEEAFYLSYHLNCLKIVDGQDCEKNFEELWQYMKSQKATFPE 120
           FGRPIVSAEKDKQWFQ GMEEAFYLSYHLNCLKIVDGQDCEKNF+ELW YMKSQKATFPE
Sbjct: 61  FGRPIVSAEKDKQWFQFGMEEAFYLSYHLNCLKIVDGQDCEKNFDELWLYMKSQKATFPE 120

Query: 121 FYKAYQNLRMKNWVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEGGGSNENGRLRVWS 180
           FYKAYQNLRMKNWVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEG G NENGRLRVWS
Sbjct: 121 FYKAYQNLRMKNWVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEGEGINENGRLRVWS 180

Query: 181 DFLCTIRLCGSVAKTLLVLTVNDNGSGSVSPSCLDSYSIEERTVTRWSPEQCRENPA 238
           DFLCTIRLCGSVAKTLLVLTVN NG+ SVSPSCLDSYSIEERTVTRWSPEQCRENPA
Sbjct: 181 DFLCTIRLCGSVAKTLLVLTVNSNGNRSVSPSCLDSYSIEERTVTRWSPEQCRENPA 237

[Close]
BLAST of Tan0020634 vs. NCBI nr
Match: KAG7035455.1 (tRNA-splicing endonuclease subunit Sen2-2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 462.6 bits (1189), Expect = 2.0e-126
Identity = 226/237 (95.36%), Postives = 229/237 (96.62%), Query Frame = 0 

Query: 1   MGPRWKGKGSEAKALADPMSKIVLQLQSSLTQSDAQGLLSSSSVLYQANEEQAELLNRAC 60
           MG RWKGKGSEAKALADPMSKIVLQLQSSLTQSDAQGLLSS SVLYQA+EEQAELLNRAC
Sbjct: 1   MGLRWKGKGSEAKALADPMSKIVLQLQSSLTQSDAQGLLSSCSVLYQADEEQAELLNRAC 60

Query: 61  FGRPIVSAEKDKQWFQLGMEEAFYLSYHLNCLKIVDGQDCEKNFEELWQYMKSQKATFPE 120
           FGRPIVSAEKDKQWFQ GMEEAFYLSYHLNCLKIVDGQDCEKNF+ELW YMKSQKATFPE
Sbjct: 61  FGRPIVSAEKDKQWFQFGMEEAFYLSYHLNCLKIVDGQDCEKNFDELWLYMKSQKATFPE 120

Query: 121 FYKAYQNLRMKNWVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEGGGSNENGRLRVWS 180
           FYKAYQNLRMKNWVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEG G NENGRLRVWS
Sbjct: 121 FYKAYQNLRMKNWVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEGEGINENGRLRVWS 180

Query: 181 DFLCTIRLCGSVAKTLLVLTVNDNGSGSVSPSCLDSYSIEERTVTRWSPEQCRENPA 238
           DFLCTIRLCGSVAKTLLVLTVN NG+ SVSPSCLDSYSIEERTVTRWSPEQCRENPA
Sbjct: 181 DFLCTIRLCGSVAKTLLVLTVNSNGNRSVSPSCLDSYSIEERTVTRWSPEQCRENPA 237

[Close]
BLAST of Tan0020634 vs. ExPASy TrEMBL
Match: A0A6J1G716 (tRNA-intron lyase OS=Cucurbita moschata OX=3662 GN=LOC111451414 PE=3 SV=1)

HSP 1 Score: 464.9 bits (1195), Expect = 2.0e-127
Identity = 226/237 (95.36%), Postives = 231/237 (97.47%), Query Frame = 0 

Query: 1   MGPRWKGKGSEAKALADPMSKIVLQLQSSLTQSDAQGLLSSSSVLYQANEEQAELLNRAC 60
           MGPRWKGKGSEAKALADP+SKIVLQLQSSLTQSDAQGLLSS SVLYQA+EEQAELLNRAC
Sbjct: 1   MGPRWKGKGSEAKALADPVSKIVLQLQSSLTQSDAQGLLSSCSVLYQADEEQAELLNRAC 60

Query: 61  FGRPIVSAEKDKQWFQLGMEEAFYLSYHLNCLKIVDGQDCEKNFEELWQYMKSQKATFPE 120
           FGRPIVSAEKDKQWFQ GMEEAFYLSY+LNCLKIVDGQDCEKNF+ELW YMKSQKATFPE
Sbjct: 61  FGRPIVSAEKDKQWFQFGMEEAFYLSYYLNCLKIVDGQDCEKNFDELWLYMKSQKATFPE 120

Query: 121 FYKAYQNLRMKNWVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEGGGSNENGRLRVWS 180
           FYKAYQNLRMKNWVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEG GSNENGRLRVWS
Sbjct: 121 FYKAYQNLRMKNWVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEGEGSNENGRLRVWS 180

Query: 181 DFLCTIRLCGSVAKTLLVLTVNDNGSGSVSPSCLDSYSIEERTVTRWSPEQCRENPA 238
           DFLCTIRLCGSVAKTLLVLTVN NG+ SVSPSCLDSYSIEERTVTRWSPEQCRENPA
Sbjct: 181 DFLCTIRLCGSVAKTLLVLTVNSNGNRSVSPSCLDSYSIEERTVTRWSPEQCRENPA 237

[Close]
BLAST of Tan0020634 vs. ExPASy TrEMBL
Match: A0A6J1G6W2 (tRNA-intron lyase OS=Cucurbita moschata OX=3662 GN=LOC111451414 PE=3 SV=1)

HSP 1 Score: 464.9 bits (1195), Expect = 2.0e-127
Identity = 226/237 (95.36%), Postives = 231/237 (97.47%), Query Frame = 0 

Query: 1   MGPRWKGKGSEAKALADPMSKIVLQLQSSLTQSDAQGLLSSSSVLYQANEEQAELLNRAC 60
           MGPRWKGKGSEAKALADP+SKIVLQLQSSLTQSDAQGLLSS SVLYQA+EEQAELLNRAC
Sbjct: 1   MGPRWKGKGSEAKALADPVSKIVLQLQSSLTQSDAQGLLSSCSVLYQADEEQAELLNRAC 60

Query: 61  FGRPIVSAEKDKQWFQLGMEEAFYLSYHLNCLKIVDGQDCEKNFEELWQYMKSQKATFPE 120
           FGRPIVSAEKDKQWFQ GMEEAFYLSY+LNCLKIVDGQDCEKNF+ELW YMKSQKATFPE
Sbjct: 61  FGRPIVSAEKDKQWFQFGMEEAFYLSYYLNCLKIVDGQDCEKNFDELWLYMKSQKATFPE 120

Query: 121 FYKAYQNLRMKNWVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEGGGSNENGRLRVWS 180
           FYKAYQNLRMKNWVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEG GSNENGRLRVWS
Sbjct: 121 FYKAYQNLRMKNWVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEGEGSNENGRLRVWS 180

Query: 181 DFLCTIRLCGSVAKTLLVLTVNDNGSGSVSPSCLDSYSIEERTVTRWSPEQCRENPA 238
           DFLCTIRLCGSVAKTLLVLTVN NG+ SVSPSCLDSYSIEERTVTRWSPEQCRENPA
Sbjct: 181 DFLCTIRLCGSVAKTLLVLTVNSNGNRSVSPSCLDSYSIEERTVTRWSPEQCRENPA 237

[Close]
BLAST of Tan0020634 vs. ExPASy TrEMBL
Match: A0A6J1L2V2 (tRNA-intron lyase OS=Cucurbita maxima OX=3661 GN=LOC111499907 PE=3 SV=1)

HSP 1 Score: 457.6 bits (1176), Expect = 3.2e-125
Identity = 223/237 (94.09%), Postives = 225/237 (94.94%), Query Frame = 0 

Query: 1   MGPRWKGKGSEAKALADPMSKIVLQLQSSLTQSDAQGLLSSSSVLYQANEEQAELLNRAC 60
           MGPRWKGKGSEAKALADPMSKIV QLQSSL QSDAQGLLSS SVLYQA+EEQAELLNRAC
Sbjct: 1   MGPRWKGKGSEAKALADPMSKIVCQLQSSLIQSDAQGLLSSCSVLYQADEEQAELLNRAC 60

Query: 61  FGRPIVSAEKDKQWFQLGMEEAFYLSYHLNCLKIVDGQDCEKNFEELWQYMKSQKATFPE 120
           FGRPIVSAEKDKQWFQ GMEEAFYL YHLNCLKIVDGQDCE NFEELW YMKSQ ATFPE
Sbjct: 61  FGRPIVSAEKDKQWFQFGMEEAFYLCYHLNCLKIVDGQDCETNFEELWHYMKSQNATFPE 120

Query: 121 FYKAYQNLRMKNWVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEGGGSNENGRLRVWS 180
           FYKAYQNLRMKNWVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEG GSNENGRLRVWS
Sbjct: 121 FYKAYQNLRMKNWVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEGEGSNENGRLRVWS 180

Query: 181 DFLCTIRLCGSVAKTLLVLTVNDNGSGSVSPSCLDSYSIEERTVTRWSPEQCRENPA 238
           DFLCTIRLCGSVAKTLLVLTVN NG+ SVSPSCLDSYSIEERTVT WSPEQCRENPA
Sbjct: 181 DFLCTIRLCGSVAKTLLVLTVNSNGNRSVSPSCLDSYSIEERTVTGWSPEQCRENPA 237

[Close]
BLAST of Tan0020634 vs. ExPASy TrEMBL
Match: A0A6J1L4U3 (tRNA-intron lyase OS=Cucurbita maxima OX=3661 GN=LOC111499907 PE=3 SV=1)

HSP 1 Score: 457.6 bits (1176), Expect = 3.2e-125
Identity = 223/237 (94.09%), Postives = 225/237 (94.94%), Query Frame = 0 

Query: 1   MGPRWKGKGSEAKALADPMSKIVLQLQSSLTQSDAQGLLSSSSVLYQANEEQAELLNRAC 60
           MGPRWKGKGSEAKALADPMSKIV QLQSSL QSDAQGLLSS SVLYQA+EEQAELLNRAC
Sbjct: 1   MGPRWKGKGSEAKALADPMSKIVCQLQSSLIQSDAQGLLSSCSVLYQADEEQAELLNRAC 60

Query: 61  FGRPIVSAEKDKQWFQLGMEEAFYLSYHLNCLKIVDGQDCEKNFEELWQYMKSQKATFPE 120
           FGRPIVSAEKDKQWFQ GMEEAFYL YHLNCLKIVDGQDCE NFEELW YMKSQ ATFPE
Sbjct: 61  FGRPIVSAEKDKQWFQFGMEEAFYLCYHLNCLKIVDGQDCETNFEELWHYMKSQNATFPE 120

Query: 121 FYKAYQNLRMKNWVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEGGGSNENGRLRVWS 180
           FYKAYQNLRMKNWVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEG GSNENGRLRVWS
Sbjct: 121 FYKAYQNLRMKNWVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEGEGSNENGRLRVWS 180

Query: 181 DFLCTIRLCGSVAKTLLVLTVNDNGSGSVSPSCLDSYSIEERTVTRWSPEQCRENPA 238
           DFLCTIRLCGSVAKTLLVLTVN NG+ SVSPSCLDSYSIEERTVT WSPEQCRENPA
Sbjct: 181 DFLCTIRLCGSVAKTLLVLTVNSNGNRSVSPSCLDSYSIEERTVTGWSPEQCRENPA 237

[Close]
BLAST of Tan0020634 vs. ExPASy TrEMBL
Match: A0A6J1CYW3 (tRNA-intron lyase OS=Momordica charantia OX=3673 GN=LOC111015540 PE=3 SV=1)

HSP 1 Score: 449.9 bits (1156), Expect = 6.6e-123
Identity = 220/240 (91.67%), Postives = 230/240 (95.83%), Query Frame = 0 

Query: 1   MGPRWKGKGSEAKALADPMSKIVLQLQSSLTQSDAQGLLSSSSVLYQANEEQAELLNRAC 60
           MGPRWKGKGSEAKALADPMSKIVLQLQSSLTQSDAQGLLS  SVLYQA+EEQ +L NRAC
Sbjct: 1   MGPRWKGKGSEAKALADPMSKIVLQLQSSLTQSDAQGLLSGCSVLYQADEEQTDLFNRAC 60

Query: 61  FGRPIVSAEKDKQWFQLGMEEAFYLSYHLNCLKIVDGQDCEKNFEELWQYMKSQKATFPE 120
           FGRPIV+A+KDKQWFQLG+EEAFYLS HL CLKIVDGQDCEKN E+LW+YMKSQKATFPE
Sbjct: 61  FGRPIVTADKDKQWFQLGVEEAFYLSCHLKCLKIVDGQDCEKNAEQLWEYMKSQKATFPE 120

Query: 121 FYKAYQNLRMKNWVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEGGG-SNENGRLRVW 180
           FYKAYQ+LRMKNWVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEGGG SNENGRLRVW
Sbjct: 121 FYKAYQHLRMKNWVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEGGGDSNENGRLRVW 180

Query: 181 SDFLCTIRLCGSVAKTLLVLTVNDNGSGSVSPSCLDSYSIEERTVTRWSPEQCRENPAKV 240
           SDFLCTIRLCGSVAKTLLVL+VN+NGS SVSPS LDSYSIEERTVTRWSPEQCRENPAKV
Sbjct: 181 SDFLCTIRLCGSVAKTLLVLSVNNNGSSSVSPSFLDSYSIEERTVTRWSPEQCRENPAKV 240

[Close]
BLAST of Tan0020634 vs. TAIR 10
Match: AT3G45590.1 (splicing endonuclease 1 )

HSP 1 Score: 268.1 bits (684), Expect = 6.9e-72
Identity = 140/241 (58.09%), Postives = 173/241 (71.78%), Query Frame = 0 

Query: 1   MGPRWKGKGSEAKALADPMSKIVLQLQSSLTQSDAQGLLSSSSVLYQANEEQAELLNRAC 60
           M PRWK KG+EAKALA+P+SK V +L+SSLTQ++A G LSS +VL     E+AELL+R C
Sbjct: 1   MAPRWKWKGAEAKALAEPVSKTVSELRSSLTQTEALGFLSSCNVLLSVESEEAELLDRCC 60

Query: 61  FGRPIVSAEKDKQWFQLGMEEAFYLSYHLNCLKI-VDGQDCEKNFEELWQYMKSQKATFP 120
           FGR +V AEKDK+W QL  EEAF+L Y L C+KI + G+  E N  +LW+ M S K  F 
Sbjct: 61  FGRLVVGAEKDKRWIQLSFEEAFFLFYKLKCIKICLHGRSLE-NEVDLWRSMSSFKQDFA 120

Query: 121 EFYKAYQNLRMKNWVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEGGGSNENGRLRVW 180
             YKAY +LR KNW+VR G QYGVDFV YRHHP+LVHSE+AVLV S GG    N RL+VW
Sbjct: 121 ILYKAYSHLRSKNWIVRSGLQYGVDFVVYRHHPSLVHSEYAVLVQSIGG----NDRLKVW 180

Query: 181 SDFLCTIRLCGSVAKTLLVLTVND--NGSGSVSPSCLDSYSIEERTVTRWSPEQCRENPA 239
           SD  C++RL GSVAK+LLVL VN   N      P CL+ Y++EE+T+ RWSPE  RE+  
Sbjct: 181 SDIHCSVRLTGSVAKSLLVLYVNRKVNTEKMNLPLCLEDYTVEEQTIRRWSPELSREDET 236

[Close]
BLAST of Tan0020634 vs. TAIR 10
Match: AT3G45590.2 (splicing endonuclease 1 )

HSP 1 Score: 268.1 bits (684), Expect = 6.9e-72
Identity = 140/241 (58.09%), Postives = 173/241 (71.78%), Query Frame = 0 

Query: 1   MGPRWKGKGSEAKALADPMSKIVLQLQSSLTQSDAQGLLSSSSVLYQANEEQAELLNRAC 60
           M PRWK KG+EAKALA+P+SK V +L+SSLTQ++A G LSS +VL     E+AELL+R C
Sbjct: 1   MAPRWKWKGAEAKALAEPVSKTVSELRSSLTQTEALGFLSSCNVLLSVESEEAELLDRCC 60

Query: 61  FGRPIVSAEKDKQWFQLGMEEAFYLSYHLNCLKI-VDGQDCEKNFEELWQYMKSQKATFP 120
           FGR +V AEKDK+W QL  EEAF+L Y L C+KI + G+  E N  +LW+ M S K  F 
Sbjct: 61  FGRLVVGAEKDKRWIQLSFEEAFFLFYKLKCIKICLHGRSLE-NEVDLWRSMSSFKQDFA 120

Query: 121 EFYKAYQNLRMKNWVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEGGGSNENGRLRVW 180
             YKAY +LR KNW+VR G QYGVDFV YRHHP+LVHSE+AVLV S GG    N RL+VW
Sbjct: 121 ILYKAYSHLRSKNWIVRSGLQYGVDFVVYRHHPSLVHSEYAVLVQSIGG----NDRLKVW 180

Query: 181 SDFLCTIRLCGSVAKTLLVLTVND--NGSGSVSPSCLDSYSIEERTVTRWSPEQCRENPA 239
           SD  C++RL GSVAK+LLVL VN   N      P CL+ Y++EE+T+ RWSPE  RE+  
Sbjct: 181 SDIHCSVRLTGSVAKSLLVLYVNRKVNTEKMNLPLCLEDYTVEEQTIRRWSPELSREDET 236

[Close]
BLAST of Tan0020634 vs. TAIR 10
Match: AT5G60230.1 (splicing endonuclease 2 )

HSP 1 Score: 266.9 bits (681), Expect = 1.5e-71
Identity = 134/237 (56.54%), Postives = 172/237 (72.57%), Query Frame = 0 

Query: 1   MGPRWKGKGSEAKALADPMSKIVLQLQSSLTQSDAQGLLSSSSVLYQANEEQAELLNRAC 60
           M PRWK KG+EAKALA+P+SK V +LQ SL ++++ G LSS +VL     EQAELL+R C
Sbjct: 1   MAPRWKWKGAEAKALAEPISKSVSELQLSLAETESSGTLSSCNVLLAVEPEQAELLDRCC 60

Query: 61  FGRPIVSAEKDKQWFQLGMEEAFYLSYHLNCLKIVDGQDCEKNFEELWQYMKSQKATFPE 120
           FGR ++SAEK K+W QL  EEAF+L Y+L C+KI     C +N  + W YMKS++  FP 
Sbjct: 61  FGRLVLSAEKIKKWIQLSFEEAFFLHYNLKCIKISLQGRCLENEVDTWLYMKSKRPNFPM 120

Query: 121 FYKAYQNLRMKNWVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEGGGSNENGRLRVWS 180
           F+KAY +LR KNWV+R G QYGVDFVAYRHHP+LVHSE++VLV      S ++ RLRVWS
Sbjct: 121 FFKAYSHLRSKNWVLRSGLQYGVDFVAYRHHPSLVHSEYSVLV-----QSGDSDRLRVWS 180

Query: 181 DFLCTIRLCGSVAKTLLVLTVNDNGSGSVSP--SCLDSYSIEERTVTRWSPEQCREN 236
           D  C +RL GSVAKTLL L VN N  G       CL+++++EE+T++RWSPE  RE+
Sbjct: 181 DIHCAVRLSGSVAKTLLTLYVNGNFKGEDVNLLVCLENFTVEEQTISRWSPELSRED 232

[Close]
BLAST of Tan0020634 vs. TAIR 10
Match: AT5G60230.2 (splicing endonuclease 2 )

HSP 1 Score: 266.9 bits (681), Expect = 1.5e-71
Identity = 134/237 (56.54%), Postives = 172/237 (72.57%), Query Frame = 0 

Query: 1   MGPRWKGKGSEAKALADPMSKIVLQLQSSLTQSDAQGLLSSSSVLYQANEEQAELLNRAC 60
           M PRWK KG+EAKALA+P+SK V +LQ SL ++++ G LSS +VL     EQAELL+R C
Sbjct: 6   MAPRWKWKGAEAKALAEPISKSVSELQLSLAETESSGTLSSCNVLLAVEPEQAELLDRCC 65

Query: 61  FGRPIVSAEKDKQWFQLGMEEAFYLSYHLNCLKIVDGQDCEKNFEELWQYMKSQKATFPE 120
           FGR ++SAEK K+W QL  EEAF+L Y+L C+KI     C +N  + W YMKS++  FP 
Sbjct: 66  FGRLVLSAEKIKKWIQLSFEEAFFLHYNLKCIKISLQGRCLENEVDTWLYMKSKRPNFPM 125

Query: 121 FYKAYQNLRMKNWVVRPGSQYGVDFVAYRHHPALVHSEFAVLVLSEGGGSNENGRLRVWS 180
           F+KAY +LR KNWV+R G QYGVDFVAYRHHP+LVHSE++VLV      S ++ RLRVWS
Sbjct: 126 FFKAYSHLRSKNWVLRSGLQYGVDFVAYRHHPSLVHSEYSVLV-----QSGDSDRLRVWS 185

Query: 181 DFLCTIRLCGSVAKTLLVLTVNDNGSGSVSP--SCLDSYSIEERTVTRWSPEQCREN 236
           D  C +RL GSVAKTLL L VN N  G       CL+++++EE+T++RWSPE  RE+
Sbjct: 186 DIHCAVRLSGSVAKTLLTLYVNGNFKGEDVNLLVCLENFTVEEQTISRWSPELSRED 237

[Close]
BLAST of Tan0020634 vs. TAIR 10
Match: AT3G45577.1 (tRNA-intron endonucleases )

HSP 1 Score: 88.2 bits (217), Expect = 9.8e-18
Identity = 49/98 (50.00%), Postives = 60/98 (61.22%), Query Frame = 0 

Query: 35  AQGLLSSSSVLYQANEEQAELLNRACFGRPIVSAEKDKQWFQLGMEEAFYLSYHLNCLKI 94
           A G LSS +V      EQAELL+R CFGR +  AEKDK+W QL  EEAF+L Y L C+KI
Sbjct: 24  ALGFLSSCNVFL---SEQAELLDRYCFGRLVFGAEKDKRWIQLSYEEAFFLFYKLKCIKI 83

Query: 95  VDGQDCEKNFEELWQYMKSQKATFPEFYKAYQNLRMKN 133
                   N  +LW+ ++S K  F   YKAY +LR KN
Sbjct: 84  SLHGRSLVNGVDLWRSIRSFKPNFTILYKAYSHLRSKN 118

The following BLAST results are available for this feature:
BLAST of Tan0020634 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9M1E89.7e-7158.09tRNA-splicing endonuclease subunit Sen2-1 OS=Arabidopsis thaliana OX=3702 GN=SEN... [more]
Q9LSS32.2e-7056.54tRNA-splicing endonuclease subunit Sen2-2 OS=Arabidopsis thaliana OX=3702 GN=SEN... [more]
Q5Z6B11.1e-6151.87Probable tRNA-splicing endonuclease subunit Sen2 OS=Oryza sativa subsp. japonica... [more]
Q9P6Y21.2e-0926.56Probable tRNA-splicing endonuclease subunit tsp-2 OS=Neurospora crassa (strain A... [more]
Q97ZY34.7e-0929.10tRNA-splicing endonuclease OS=Saccharolobus solfataricus (strain ATCC 35092 / DS... [more]
BLAST of Tan0020634 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_023532286.11.7e-12896.20tRNA-splicing endonuclease subunit Sen2-1-like [Cucurbita pepo subsp. pepo][more]
XP_022947593.14.1e-12795.36tRNA-splicing endonuclease subunit Sen2-1-like isoform X2 [Cucurbita moschata][more]
XP_022947592.14.1e-12795.36tRNA-splicing endonuclease subunit Sen2-1-like isoform X1 [Cucurbita moschata][more]
KAG6605517.12.0e-12695.36tRNA-splicing endonuclease subunit Sen2-2, partial [Cucurbita argyrosperma subsp... [more]
KAG7035455.12.0e-12695.36tRNA-splicing endonuclease subunit Sen2-2, partial [Cucurbita argyrosperma subsp... [more]
BLAST of Tan0020634 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1G7162.0e-12795.36tRNA-intron lyase OS=Cucurbita moschata OX=3662 GN=LOC111451414 PE=3 SV=1[more]
A0A6J1G6W22.0e-12795.36tRNA-intron lyase OS=Cucurbita moschata OX=3662 GN=LOC111451414 PE=3 SV=1[more]
A0A6J1L2V23.2e-12594.09tRNA-intron lyase OS=Cucurbita maxima OX=3661 GN=LOC111499907 PE=3 SV=1[more]
A0A6J1L4U33.2e-12594.09tRNA-intron lyase OS=Cucurbita maxima OX=3661 GN=LOC111499907 PE=3 SV=1[more]
A0A6J1CYW36.6e-12391.67tRNA-intron lyase OS=Momordica charantia OX=3673 GN=LOC111015540 PE=3 SV=1[more]
BLAST of Tan0020634 vs. TAIR 10
Match NameE-valueIdentityDescription
AT3G45590.16.9e-7258.09splicing endonuclease 1 [more]
AT3G45590.26.9e-7258.09splicing endonuclease 1 [more]
AT5G60230.11.5e-7156.54splicing endonuclease 2 [more]
AT5G60230.21.5e-7156.54splicing endonuclease 2 [more]
AT3G45577.19.8e-1850.00tRNA-intron endonucleases [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006677tRNA intron endonuclease, catalytic domain-likePFAMPF01974tRNA_int_endocoord: 121..205
e-value: 1.3E-19
score: 70.0
IPR011856tRNA endonuclease-like domain superfamilyGENE3D3.40.1350.10coord: 117..215
e-value: 1.2E-22
score: 81.7
NoneNo IPR availableGENE3D3.40.1170.20coord: 27..115
e-value: 2.9E-6
score: 28.8
NoneNo IPR availablePANTHERPTHR21227:SF2TRNA-SPLICING ENDONUCLEASE SUBUNIT SEN2-1-LIKEcoord: 1..238
IPR006678tRNA intron endonuclease, N-terminalPFAMPF02778tRNA_int_endo_Ncoord: 36..108
e-value: 3.6E-15
score: 55.3
IPR006676tRNA-splicing endonucleasePANTHERPTHR21227TRNA-SPLICING ENDONUCLEASE SUBUNIT SEN2coord: 1..238
IPR036167tRNA intron endonuclease, catalytic domain-like superfamilySUPERFAMILY53032tRNA-intron endonuclease catalytic domain-likecoord: 118..206

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0020634.1Tan0020634.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0090502 RNA phosphodiester bond hydrolysis, endonucleolytic
biological_process GO:0000379 tRNA-type intron splice site recognition and cleavage
biological_process GO:0006388 tRNA splicing, via endonucleolytic cleavage and ligation
cellular_component GO:0000214 tRNA-intron endonuclease complex
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0000213 tRNA-intron endonuclease activity
molecular_function GO:0004518 nuclease activity