Tan0019495 (gene) Snake gourd v1

BLAST of Tan0019495 vs. ExPASy Swiss-Prot
Match: P83948 (Pectinesterase 3 OS=Citrus sinensis OX=2711 PE=1 SV=1)

HSP 1 Score: 856.7 bits (2212), Expect = 1.6e-247
Identity = 438/575 (76.17%), Postives = 489/575 (85.04%), Query Frame = 0

Query: 17  NSGEQKTKKPNKNKKLIILALGSTLLLVGAVVGIVAGVSNSKTSGDRPSEA-HAILKSTC 76
           +S  Q      K KK + LAL +TLL+V AV+GIVAGV++ K SGD  +E  HAILKS+C
Sbjct: 14  SSTNQNISNIPKKKKKLFLALFATLLVVAAVIGIVAGVNSRKNSGDNGNEPHHAILKSSC 73

Query: 77  SSTLYPDLCFSSISENEGALKKVTNQKEVIELSINITVKAVEENYFRVQKLAALKNITHR 136
           SST YPDLCFS+I+    A KKVT+QK+VIE+S+NIT  AVE NYF +QKL    N+T R
Sbjct: 74  SSTRYPDLCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKLLKRTNLTKR 133

Query: 137 EKIALHDCLETIDETLDELHKAIVDLHEYPNKKSLAQHADDLKTLLSSAITNQETCLDGF 196
           EK+ALHDCLETIDETLDELHKA+ DL EYPNKKSL+QHADDLKTL+S+A+TNQ TCLDGF
Sbjct: 134 EKVALHDCLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMTNQGTCLDGF 193

Query: 197 SHDEADKNLRDKLKEGQTHVEHMCSNALAMIKNMTDTDIANYEAKMGTANRKLKEENDDH 256
           SHD+A+K++RD L +GQ HVE MCSNALAMIKNMTDTD+        + NRKL EE    
Sbjct: 194 SHDDANKHVRDALSDGQVHVEKMCSNALAMIKNMTDTDMMIMRT---SNNRKLIEETSTV 253

Query: 257 GIQWPEWLSTGDRRLLQSSSVTPDVVVAADGSGNFKTVSAAVAAAPVKSSKRYVIRIKAG 316
              WP WLSTGDRRLLQSSSVTP+VVVAADGSGNFKTV+A+VAAAP   +KRY+IRIKAG
Sbjct: 254 D-GWPAWLSTGDRRLLQSSSVTPNVVVAADGSGNFKTVAASVAAAPQGGTKRYIIRIKAG 313

Query: 317 VYRENVEVPKKKTNIMFMGDGRSNTIITGSRNVVDGSTTFNSATVAAVGEGFLARDITFE 376
           VYRENVEV KK  NIMF+GDGR+ TIITGSRNVVDGSTTF SATVA VGEGFLARDITF+
Sbjct: 314 VYRENVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATVAVVGEGFLARDITFQ 373

Query: 377 NTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINCLVAGTVDFIFGNAA 436
           NTAGPSKHQAVALRVGADLSAFY CDMLAYQDTLYVHSNRQF++NCL+AGTVDFIFGNAA
Sbjct: 374 NTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNCLIAGTVDFIFGNAA 433

Query: 437 AVFQDCDIHARKPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATSDLRPVQKNFPTFLG 496
           AV Q+CDIHARKPNSGQKNMVTAQGR+DPNQNTGIVIQKSRIGATSDL+PVQ +FPT+LG
Sbjct: 434 AVLQNCDIHARKPNSGQKNMVTAQGRADPNQNTGIVIQKSRIGATSDLKPVQGSFPTYLG 493

Query: 497 RPWKEYSRTVIMQSSISDVIDPKGWHEWSGNFALNTLFYGEYQNTGPGASTGGRVTWKGF 556
           RPWKEYSRTVIMQSSI+DVI P GWHEW GNFALNTLFYGE+QN G GA T GRV WKGF
Sbjct: 494 RPWKEYSRTVIMQSSITDVIHPAGWHEWDGNFALNTLFYGEHQNAGAGAGTSGRVKWKGF 553

Query: 557 RIIRSATEAEGFTAGKFIGGGSWLRATGFPFSLGL 591
           R+I SATEA+ FT G FI G SWL +TGFPFSLGL
Sbjct: 554 RVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584

BLAST of Tan0019495 vs. ExPASy Swiss-Prot
Match: O04886 (Pectinesterase 1 OS=Citrus sinensis OX=2711 GN=PECS-1.1 PE=2 SV=1)

HSP 1 Score: 853.6 bits (2204), Expect = 1.3e-246
Identity = 437/576 (75.87%), Postives = 490/576 (85.07%), Query Frame = 0

Query: 16  SNSGEQKTKKPNKNKKLIILALGSTLLLVGAVVGIVAGVSNSKTSGDRPSEA-HAILKST 75
           S+S +  +  P K KKL  LAL +TLL+V AV+GIVAGV++ K SGD  +E  HAILKS+
Sbjct: 14  SSSNQNISNIPKKKKKL-FLALFATLLVVAAVIGIVAGVNSRKNSGDNGNEPHHAILKSS 73

Query: 76  CSSTLYPDLCFSSISENEGALKKVTNQKEVIELSINITVKAVEENYFRVQKLAALKNITH 135
           CSST YPDLCFS+I+    A KKVT+QK+VIE+S+NIT  AVE NYF +QKL    N+T 
Sbjct: 74  CSSTRYPDLCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKLLKRTNLTK 133

Query: 136 REKIALHDCLETIDETLDELHKAIVDLHEYPNKKSLAQHADDLKTLLSSAITNQETCLDG 195
           REK+ALHDCLETIDETLDELHKA+ DL EYPNKKSL+QHADDLKTL+S+A+TNQ TCLDG
Sbjct: 134 REKVALHDCLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMTNQGTCLDG 193

Query: 196 FSHDEADKNLRDKLKEGQTHVEHMCSNALAMIKNMTDTDIANYEAKMGTANRKLKEENDD 255
           FSHD+A+K++RD L +GQ HVE MCSNALAMIKNMTDTD+        + NRKL EE   
Sbjct: 194 FSHDDANKHVRDALSDGQVHVEKMCSNALAMIKNMTDTDMMIMRT---SNNRKLTEETST 253

Query: 256 HGIQWPEWLSTGDRRLLQSSSVTPDVVVAADGSGNFKTVSAAVAAAPVKSSKRYVIRIKA 315
               WP WLS GDRRLLQSSSVTP+ VVAADGSGNFKTV+AAVAAAP   +KRY+IRIKA
Sbjct: 254 VD-GWPAWLSPGDRRLLQSSSVTPNAVVAADGSGNFKTVAAAVAAAPQGGTKRYIIRIKA 313

Query: 316 GVYRENVEVPKKKTNIMFMGDGRSNTIITGSRNVVDGSTTFNSATVAAVGEGFLARDITF 375
           GVYRENVEV KK  NIMF+GDGR+ TIITGSRNVVDGSTTF SAT A VGEGFLARDITF
Sbjct: 314 GVYRENVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATAAVVGEGFLARDITF 373

Query: 376 ENTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINCLVAGTVDFIFGNA 435
           +NTAGPSKHQAVALRVGADLSAFY CDMLAYQDTLYVHSNRQF++NCL+AGTVDFIFGNA
Sbjct: 374 QNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNCLIAGTVDFIFGNA 433

Query: 436 AAVFQDCDIHARKPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATSDLRPVQKNFPTFL 495
           AAV Q+CDIHARKPNSGQKNMVTAQGR+DPNQNTGIVIQKSRIGATSDL+PVQ +FPT+L
Sbjct: 434 AAVLQNCDIHARKPNSGQKNMVTAQGRTDPNQNTGIVIQKSRIGATSDLKPVQGSFPTYL 493

Query: 496 GRPWKEYSRTVIMQSSISDVIDPKGWHEWSGNFALNTLFYGEYQNTGPGASTGGRVTWKG 555
           GRPWKEYSRTVIMQSSI+D+I P GWHEW GNFALNTLFYGE+QN+G GA T GRV WKG
Sbjct: 494 GRPWKEYSRTVIMQSSITDLIHPAGWHEWDGNFALNTLFYGEHQNSGAGAGTSGRVKWKG 553

Query: 556 FRIIRSATEAEGFTAGKFIGGGSWLRATGFPFSLGL 591
           FR+I SATEA+ FT G FI G SWL +TGFPFSLGL
Sbjct: 554 FRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584

BLAST of Tan0019495 vs. ExPASy Swiss-Prot
Match: Q43143 (Pectinesterase/pectinesterase inhibitor U1 OS=Solanum lycopersicum OX=4081 GN=PMEU1 PE=2 SV=1)

HSP 1 Score: 819.3 bits (2115), Expect = 2.8e-236
Identity = 425/573 (74.17%), Postives = 481/573 (83.94%), Query Frame = 0

Query: 28  KNKKLIILALGSTLLLVGAVVGIVAGV-SNSKTSGDR------PSEAHAILKSTCSSTLY 87
           K KK I LA+ +++LLV AV+G+VAGV S+SK S D        S AHAI+KS CS+TL+
Sbjct: 16  KRKKKIYLAIVASVLLVAAVIGVVAGVKSHSKNSDDHADIMAISSSAHAIVKSACSNTLH 75

Query: 88  PDLCFSSISENEGALKKVTNQKEVIELSINITVKAVEENYFRVQKL-AALKNITHREKIA 147
           P+LC+S+I       KKVT+QK+VIELS+NITVKAV  NY+ V++L    K +T REK+A
Sbjct: 76  PELCYSAIVNVSDFSKKVTSQKDVIELSLNITVKAVRRNYYAVKELIKTRKGLTPREKVA 135

Query: 148 LHDCLETIDETLDELHKAIVDLHEYPNKKSLAQHADDLKTLLSSAITNQETCLDGFSHDE 207
           LHDCLET+DETLDELH A+ DL  YPNKKSL +H +DLKTL+SSAITNQETCLDGFSHDE
Sbjct: 136 LHDCLETMDETLDELHTAVEDLELYPNKKSLKEHVEDLKTLISSAITNQETCLDGFSHDE 195

Query: 208 ADKNLRDKLKEGQTHVEHMCSNALAMIKNMTDTDIANYEAKMGTA--NRKLKEENDDHGI 267
           ADK +R  L +GQ HVE MCSNALAMI NMTDTDIAN E K+     NRKL E+N     
Sbjct: 196 ADKKVRKVLLKGQKHVEKMCSNALAMICNMTDTDIAN-EMKLSAPANNRKLVEDNG---- 255

Query: 268 QWPEWLSTGDRRLLQSSSVTPDVVVAADGSGNFKTVSAAVAAAPVKSSKRYVIRIKAGVY 327
           +WPEWLS GDRRLLQSS+VTPDVVVAADGSG++KTVS AV  AP KSSKRYVIRIKAGVY
Sbjct: 256 EWPEWLSAGDRRLLQSSTVTPDVVVAADGSGDYKTVSEAVRKAPEKSSKRYVIRIKAGVY 315

Query: 328 RENVEVPKKKTNIMFMGDGRSNTIITGSRNVVDGSTTFNSATVAAVGEGFLARDITFENT 387
           RENV+VPKKKTNIMFMGDG+SNTIIT SRNV DGSTTF+SATV  V    LARDITF+NT
Sbjct: 316 RENVDVPKKKTNIMFMGDGKSNTIITASRNVQDGSTTFHSATVVRVAGKVLARDITFQNT 375

Query: 388 AGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINCLVAGTVDFIFGNAAAV 447
           AG SKHQAVAL VG+DLSAFY+CDMLAYQDTLYVHSNRQF++ CLVAGTVDFIFGN AAV
Sbjct: 376 AGASKHQAVALCVGSDLSAFYRCDMLAYQDTLYVHSNRQFFVQCLVAGTVDFIFGNGAAV 435

Query: 448 FQDCDIHARKPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATSDLRPVQKNFPTFLGRP 507
           FQDCDIHAR+P SGQKNMVTAQGR+DPNQNTGIVIQK RIGATSDLRPVQK+FPT+LGRP
Sbjct: 436 FQDCDIHARRPGSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATSDLRPVQKSFPTYLGRP 495

Query: 508 WKEYSRTVIMQSSISDVIDPKGWHEWSGNFALNTLFYGEYQNTGPGASTGGRVTWKGFRI 567
           WKEYSRTVIMQSSI+DVI P GWHEW+GNFAL+TLFYGEY NTG GA T GRV WKG ++
Sbjct: 496 WKEYSRTVIMQSSITDVIQPAGWHEWNGNFALDTLFYGEYANTGAGAPTSGRVKWKGHKV 555

Query: 568 IRSATEAEGFTAGKFIGGGSWLRATGFPFSLGL 591
           I S+TEA+ +T G+FI GGSWL +TGFPFSLGL
Sbjct: 556 ITSSTEAQAYTPGRFIAGGSWLSSTGFPFSLGL 583

BLAST of Tan0019495 vs. ExPASy Swiss-Prot
Match: O49006 (Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PME3 PE=1 SV=2)

HSP 1 Score: 799.7 bits (2064), Expect = 2.3e-230
Identity = 408/579 (70.47%), Postives = 468/579 (80.83%), Query Frame = 0

Query: 28  KNKKLIILALGSTLLLVGAVVGIVAGVSNSKTSGDRPSEAHAILKSTCSSTLYPDLCFSS 87
           KNKKL++L+    LL V AV GI AG S +         +HA+L+S+CSST YP+LC S+
Sbjct: 16  KNKKLVLLSAAVALLFVAAVAGISAGASKANEKRTLSPSSHAVLRSSCSSTRYPELCISA 75

Query: 88  ISENEGALKKVTNQKEVIELSINITVKAVEENYFRVQKL-AALKNITHREKIALHDCLET 147
           +    G   ++T+QK+VIE S+N+T+ AVE NYF V+KL    K +T REK ALHDCLET
Sbjct: 76  VVTAGGV--ELTSQKDVIEASVNLTITAVEHNYFTVKKLIKKRKGLTPREKTALHDCLET 135

Query: 148 IDETLDELHKAIVDLHEYPNKKSLAQHADDLKTLLSSAITNQETCLDGFSHDEADKNLRD 207
           IDETLDELH+ + DLH YP KK+L +HA DLKTL+SSAITNQETCLDGFSHD+ADK +R 
Sbjct: 136 IDETLDELHETVEDLHLYPTKKTLREHAGDLKTLISSAITNQETCLDGFSHDDADKQVRK 195

Query: 208 KLKEGQTHVEHMCSNALAMIKNMTDTDIANYE--AKMGTANRKLKEENDDHGIQ------ 267
            L +GQ HVEHMCSNALAMIKNMTDTDIAN+E  AK+ + NRKLKEEN +  +       
Sbjct: 196 ALLKGQIHVEHMCSNALAMIKNMTDTDIANFEQKAKITSNNRKLKEENQETTVAVDIAGA 255

Query: 268 -------WPEWLSTGDRRLLQSSSVTPDVVVAADGSGNFKTVSAAVAAAPVKSSKRYVIR 327
                  WP WLS GDRRLLQ S V  D  VAADGSG FKTV+AAVAAAP  S+KRYVI 
Sbjct: 256 GELDSEGWPTWLSAGDRRLLQGSGVKADATVAADGSGTFKTVAAAVAAAPENSNKRYVIH 315

Query: 328 IKAGVYRENVEVPKKKTNIMFMGDGRSNTIITGSRNVVDGSTTFNSATVAAVGEGFLARD 387
           IKAGVYRENVEV KKK NIMFMGDGR+ TIITGSRNVVDGSTTF+SATVAAVGE FLARD
Sbjct: 316 IKAGVYRENVEVAKKKKNIMFMGDGRTRTIITGSRNVVDGSTTFHSATVAAVGERFLARD 375

Query: 388 ITFENTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINCLVAGTVDFIF 447
           ITF+NTAGPSKHQAVALRVG+D SAFY CDMLAYQDTLYVHSNRQF++ CL+AGTVDFIF
Sbjct: 376 ITFQNTAGPSKHQAVALRVGSDFSAFYNCDMLAYQDTLYVHSNRQFFVKCLIAGTVDFIF 435

Query: 448 GNAAAVFQDCDIHARKPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATSDLRPVQKNFP 507
           GNAA V QDCDIHAR+PNSGQKNMVTAQGR+DPNQNTGIVIQK RIGATSDL+ V+ +FP
Sbjct: 436 GNAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATSDLQSVKGSFP 495

Query: 508 TFLGRPWKEYSRTVIMQSSISDVIDPKGWHEWSGNFALNTLFYGEYQNTGPGASTGGRVT 567
           T+LGRPWKEYS+TVIMQS+ISDVI P+GW EW+G FALNTL Y EY NTG GA T  RV 
Sbjct: 496 TYLGRPWKEYSQTVIMQSAISDVIRPEGWSEWTGTFALNTLTYREYSNTGAGAGTANRVK 555

Query: 568 WKGFRIIRSATEAEGFTAGKFIGGGSWLRATGFPFSLGL 591
           W+GF++I +A EA+ +TAG+FIGGG WL +TGFPFSLGL
Sbjct: 556 WRGFKVITAAAEAQKYTAGQFIGGGGWLSSTGFPFSLGL 592

BLAST of Tan0019495 vs. ExPASy Swiss-Prot
Match: Q42534 (Pectinesterase 2 OS=Arabidopsis thaliana OX=3702 GN=PME2 PE=2 SV=2)

HSP 1 Score: 780.8 bits (2015), Expect = 1.1e-224
Identity = 403/573 (70.33%), Postives = 463/573 (80.80%), Query Frame = 0

Query: 29  NKKLIILALGSTLLLVGAVVGIVAGVSNSKTS---GDRPSEAHAILKSTCSSTLYPDLCF 88
           NKKLI+ +    LLL+ ++VGI A  +N   +       S +HAILKS CSSTLYP+LCF
Sbjct: 17  NKKLILSSAAIALLLLASIVGIAATTTNQNKNQKITTLSSTSHAILKSVCSSTLYPELCF 76

Query: 89  SSISENEGALKKVTNQKEVIELSINITVKAVEENYFRVQKL-AALKNITHREKIALHDCL 148
           S+++   G  K++T+QKEVIE S+N+T KAV+ NYF V+KL A  K +T RE  ALHDCL
Sbjct: 77  SAVAATGG--KELTSQKEVIEASLNLTTKAVKHNYFAVKKLIAKRKGLTPREVTALHDCL 136

Query: 149 ETIDETLDELHKAIVDLHEYPNKKSLAQHADDLKTLLSSAITNQETCLDGFSHDEADKNL 208
           ETIDETLDELH A+ DLH+YP +KSL +HADDLKTL+SSAITNQ TCLDGFS+D+AD+ +
Sbjct: 137 ETIDETLDELHVAVEDLHQYPKQKSLRKHADDLKTLISSAITNQGTCLDGFSYDDADRKV 196

Query: 209 RDKLKEGQTHVEHMCSNALAMIKNMTDTDIANYEAK------MGTANRKLKEENDD-HGI 268
           R  L +GQ HVEHMCSNALAMIKNMT+TDIAN+E +          NRKLKE   D    
Sbjct: 197 RKALLKGQVHVEHMCSNALAMIKNMTETDIANFELRDKSSTFTNNNNRKLKEVTGDLDSD 256

Query: 269 QWPEWLSTGDRRLLQSSSVTPDVVVAADGSGNFKTVSAAVAAAPVKSSKRYVIRIKAGVY 328
            WP+WLS GDRRLLQ S++  D  VA DGSG+F TV+AAVAAAP KS+KR+VI IKAGVY
Sbjct: 257 GWPKWLSVGDRRLLQGSTIKADATVADDGSGDFTTVAAAVAAAPEKSNKRFVIHIKAGVY 316

Query: 329 RENVEVPKKKTNIMFMGDGRSNTIITGSRNVVDGSTTFNSATVAAVGEGFLARDITFENT 388
           RENVEV KKKTNIMF+GDGR  TIITGSRNVVDGSTTF+SATVAAVGE FLARDITF+NT
Sbjct: 317 RENVEVTKKKTNIMFLGDGRGKTIITGSRNVVDGSTTFHSATVAAVGERFLARDITFQNT 376

Query: 389 AGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINCLVAGTVDFIFGNAAAV 448
           AGPSKHQAVALRVG+D SAFYQCDM AYQDTLYVHSNRQF++ C + GTVDFIFGNAAAV
Sbjct: 377 AGPSKHQAVALRVGSDFSAFYQCDMFAYQDTLYVHSNRQFFVKCHITGTVDFIFGNAAAV 436

Query: 449 FQDCDIHARKPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATSDLRPVQKNFPTFLGRP 508
            QDCDI+AR+PNSGQKNMVTAQGRSDPNQNTGIVIQ  RIG TSDL  V+  FPT+LGRP
Sbjct: 437 LQDCDINARRPNSGQKNMVTAQGRSDPNQNTGIVIQNCRIGGTSDLLAVKGTFPTYLGRP 496

Query: 509 WKEYSRTVIMQSSISDVIDPKGWHEWSGNFALNTLFYGEYQNTGPGASTGGRVTWKGFRI 568
           WKEYSRTVIMQS ISDVI P+GWHEWSG+FAL+TL Y EY N G GA T  RV WKG+++
Sbjct: 497 WKEYSRTVIMQSDISDVIRPEGWHEWSGSFALDTLTYREYLNRGGGAGTANRVKWKGYKV 556

Query: 569 IRSATEAEGFTAGKFIGGGSWLRATGFPFSLGL 591
           I S TEA+ FTAG+FIGGG WL +TGFPFSL L
Sbjct: 557 ITSDTEAQPFTAGQFIGGGGWLASTGFPFSLSL 587

BLAST of Tan0019495 vs. NCBI nr
Match: XP_022992216.1 (pectinesterase-like [Cucurbita maxima])

HSP 1 Score: 1013.8 bits (2620), Expect = 5.9e-292
Identity = 518/585 (88.55%), Postives = 545/585 (93.16%), Query Frame = 0

Query: 7   MEKPIAAGISNSGEQKTKKPNKNKKLIILALGSTLLLVGAVVGIVAGVSNSKTSGDRPSE 66
           ME+PIAAGIS+SG    K     KKLIILA+ STLLL    VGIVAGV +S+ +G   S+
Sbjct: 1   MEQPIAAGISSSGNHLPKPKYNRKKLIILAVSSTLLL----VGIVAGVVSSRNAGGEESK 60

Query: 67  AHAILKSTCSSTLYPDLCFSSISENEGALKKVTNQKEVIELSINITVKAVEENYFRVQKL 126
           +HAI+KSTCSSTLYPDLCFSSI +N+ A+KKV+N+K+VIE+SINITVKAVEENYF+V KL
Sbjct: 61  SHAIVKSTCSSTLYPDLCFSSILKNDEAMKKVSNKKDVIEVSINITVKAVEENYFKVLKL 120

Query: 127 AALKNITHREKIALHDCLETIDETLDELHKAIVDLHEYPNKKSLAQHADDLKTLLSSAIT 186
             LKNI+HREKIALHDCLETIDETLDELHKA VDLHEYPNKKSL Q ADDLKTLLSSAIT
Sbjct: 121 YELKNISHREKIALHDCLETIDETLDELHKATVDLHEYPNKKSLTQQADDLKTLLSSAIT 180

Query: 187 NQETCLDGFSHDEADKNLRDKLKEGQTHVEHMCSNALAMIKNMTDTDIANYEAKMGT-AN 246
           NQETCLDGFSHDEADKNLRDKLKEGQ  VE MCSNALAMIKNMTDTDIANYE KMGT AN
Sbjct: 181 NQETCLDGFSHDEADKNLRDKLKEGQIEVERMCSNALAMIKNMTDTDIANYETKMGTAAN 240

Query: 247 RKLKEENDDHGIQWPEWLSTGDRRLLQSSSVTPDVVVAADGSGNFKTVSAAVAAAPVKSS 306
           RKLKEEN+D  IQWPEWLS GDRRLLQSSSV PDVVVAADGSGNFKTV+AAVAAAPVKSS
Sbjct: 241 RKLKEENNDEQIQWPEWLSAGDRRLLQSSSVKPDVVVAADGSGNFKTVAAAVAAAPVKSS 300

Query: 307 KRYVIRIKAGVYRENVEVPKKKTNIMFMGDGRSNTIITGSRNVVDGSTTFNSATVAAVGE 366
           KRYVIRIKAGVYRENV+V KKKTNIMFMGDGRSNTIITGSRNVVDGSTTFNSATVAAVGE
Sbjct: 301 KRYVIRIKAGVYRENVDVQKKKTNIMFMGDGRSNTIITGSRNVVDGSTTFNSATVAAVGE 360

Query: 367 GFLARDITFENTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINCLVAG 426
           GFLARDITF+NTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINCL++G
Sbjct: 361 GFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINCLISG 420

Query: 427 TVDFIFGNAAAVFQDCDIHARKPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATSDLRP 486
           TVDFIFGNAAAVFQDCDIHAR+PNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATSDLR 
Sbjct: 421 TVDFIFGNAAAVFQDCDIHARRPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATSDLRS 480

Query: 487 VQKNFPTFLGRPWKEYSRTVIMQSSISDVIDPKGWHEWSGNFALNTLFYGEYQNTGPGAS 546
           VQK+FPTFLGRPWKEYSRTVIMQSSISDVID KGWHEWSGNFALNTLFYGEYQNTGPGAS
Sbjct: 481 VQKSFPTFLGRPWKEYSRTVIMQSSISDVIDSKGWHEWSGNFALNTLFYGEYQNTGPGAS 540

Query: 547 TGGRVTWKGFRIIRSATEAEGFTAGKFIGGGSWLRATGFPFSLGL 591
           TGGRVTWKGFR+IRSATEAEGFT GKFIGGGSWLRATGFPFSLGL
Sbjct: 541 TGGRVTWKGFRVIRSATEAEGFTPGKFIGGGSWLRATGFPFSLGL 581

BLAST of Tan0019495 vs. NCBI nr
Match: KAG7013952.1 (hypothetical protein SDJN02_24121 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1012.7 bits (2617), Expect = 1.3e-291
Identity = 517/585 (88.38%), Postives = 545/585 (93.16%), Query Frame = 0

Query: 7   MEKPIAAGISNSGEQKTKKPNKNKKLIILALGSTLLLVGAVVGIVAGVSNSKTSGDRPSE 66
           ME+PIAAGIS+SG    K  N  KKLIILA+ STLLL    VGIVAGV +S+  G   ++
Sbjct: 1   MEQPIAAGISSSGNHLPKPKNNRKKLIILAVSSTLLL----VGIVAGVVSSRNGGGEEAK 60

Query: 67  AHAILKSTCSSTLYPDLCFSSISENEGALKKVTNQKEVIELSINITVKAVEENYFRVQKL 126
            HAI+KSTCSSTLYPDLCFSSI +++GA+K+V ++K+VIE+SINITVKAVEENYF+V KL
Sbjct: 61  RHAIVKSTCSSTLYPDLCFSSILKSDGAMKRVGSKKDVIEVSINITVKAVEENYFKVLKL 120

Query: 127 AALKNITHREKIALHDCLETIDETLDELHKAIVDLHEYPNKKSLAQHADDLKTLLSSAIT 186
             LKNI+HREKIALHDCLETIDETLDELHKA VDLHEYPNKKSL QHADDLKTLLSSAIT
Sbjct: 121 YELKNISHREKIALHDCLETIDETLDELHKATVDLHEYPNKKSLNQHADDLKTLLSSAIT 180

Query: 187 NQETCLDGFSHDEADKNLRDKLKEGQTHVEHMCSNALAMIKNMTDTDIANYEAKMG-TAN 246
           NQETCLDGFSHDEADKNLRDKLKEGQ  VE MCSNALAMIKNMTDTDIANYEAKMG TAN
Sbjct: 181 NQETCLDGFSHDEADKNLRDKLKEGQIEVERMCSNALAMIKNMTDTDIANYEAKMGTTAN 240

Query: 247 RKLKEENDDHGIQWPEWLSTGDRRLLQSSSVTPDVVVAADGSGNFKTVSAAVAAAPVKSS 306
           RKLKEE +D  IQWPEWLS GDRRLLQSSSV PDVVVAADGSGNFKTV+AAVAAAPVKSS
Sbjct: 241 RKLKEEINDEQIQWPEWLSAGDRRLLQSSSVKPDVVVAADGSGNFKTVAAAVAAAPVKSS 300

Query: 307 KRYVIRIKAGVYRENVEVPKKKTNIMFMGDGRSNTIITGSRNVVDGSTTFNSATVAAVGE 366
           KRYVIRIKAGVYRENV+VPKKKTNIMFMGDGRSNTIITGSRNVVDGSTTFNSATVAAVGE
Sbjct: 301 KRYVIRIKAGVYRENVDVPKKKTNIMFMGDGRSNTIITGSRNVVDGSTTFNSATVAAVGE 360

Query: 367 GFLARDITFENTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINCLVAG 426
           GFLARDITF+NTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINCL++G
Sbjct: 361 GFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINCLISG 420

Query: 427 TVDFIFGNAAAVFQDCDIHARKPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATSDLRP 486
           TVDFIFGNAAAVFQDCDIHAR+PNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATSDLR 
Sbjct: 421 TVDFIFGNAAAVFQDCDIHARRPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATSDLRS 480

Query: 487 VQKNFPTFLGRPWKEYSRTVIMQSSISDVIDPKGWHEWSGNFALNTLFYGEYQNTGPGAS 546
           VQK+FPTFLGRPWKEYSRTVIMQSSISDVID KGWHEWSGNFALNTLFYGEYQNTGPGAS
Sbjct: 481 VQKSFPTFLGRPWKEYSRTVIMQSSISDVIDSKGWHEWSGNFALNTLFYGEYQNTGPGAS 540

Query: 547 TGGRVTWKGFRIIRSATEAEGFTAGKFIGGGSWLRATGFPFSLGL 591
           T GRVTWKGFR+IRSATEAEGFT GKFIGGGSWLRATGFPFSLGL
Sbjct: 541 TSGRVTWKGFRVIRSATEAEGFTPGKFIGGGSWLRATGFPFSLGL 581

BLAST of Tan0019495 vs. NCBI nr
Match: XP_022953820.1 (pectinesterase-like [Cucurbita moschata])

HSP 1 Score: 1011.9 bits (2615), Expect = 2.2e-291
Identity = 516/585 (88.21%), Postives = 546/585 (93.33%), Query Frame = 0

Query: 7   MEKPIAAGISNSGEQKTKKPNKNKKLIILALGSTLLLVGAVVGIVAGVSNSKTSGDRPSE 66
           ME+PIAAGIS+SG    K  N  KKLIILA+ STLLL    VGIVAGV +S+  G   ++
Sbjct: 1   MEQPIAAGISSSGNHLPKPKNNRKKLIILAVSSTLLL----VGIVAGVVSSRNGGGEEAK 60

Query: 67  AHAILKSTCSSTLYPDLCFSSISENEGALKKVTNQKEVIELSINITVKAVEENYFRVQKL 126
           +HAI+KSTCSSTLYPDLCFSSI +++GA+K+V ++K+VIE+SINITVKAVEENYF+V KL
Sbjct: 61  SHAIVKSTCSSTLYPDLCFSSILKSDGAMKRVGSKKDVIEVSINITVKAVEENYFKVLKL 120

Query: 127 AALKNITHREKIALHDCLETIDETLDELHKAIVDLHEYPNKKSLAQHADDLKTLLSSAIT 186
             LKNI+HREKIALHDCLETIDETLDELHKA VDLHEYPNKKSL QHADDLKTLLSSAIT
Sbjct: 121 YELKNISHREKIALHDCLETIDETLDELHKATVDLHEYPNKKSLNQHADDLKTLLSSAIT 180

Query: 187 NQETCLDGFSHDEADKNLRDKLKEGQTHVEHMCSNALAMIKNMTDTDIANYEAKMG-TAN 246
           NQETCLDGFSHDEADKNLRDKLKEGQ  VE MCSNALAMIKNMTDTDIANYEAKMG TAN
Sbjct: 181 NQETCLDGFSHDEADKNLRDKLKEGQIEVERMCSNALAMIKNMTDTDIANYEAKMGTTAN 240

Query: 247 RKLKEENDDHGIQWPEWLSTGDRRLLQSSSVTPDVVVAADGSGNFKTVSAAVAAAPVKSS 306
           RKLKEE +D  IQWPEWLS GDRRLLQSSSV PDVVVAADGSGNFKTV+AAVAAAPVKSS
Sbjct: 241 RKLKEEINDEQIQWPEWLSAGDRRLLQSSSVKPDVVVAADGSGNFKTVAAAVAAAPVKSS 300

Query: 307 KRYVIRIKAGVYRENVEVPKKKTNIMFMGDGRSNTIITGSRNVVDGSTTFNSATVAAVGE 366
           KR+VIRIKAGVYRENV+VPKKKTNIMFMGDGRSNTIITGSRNVVDGSTTFNSATVAAVGE
Sbjct: 301 KRFVIRIKAGVYRENVDVPKKKTNIMFMGDGRSNTIITGSRNVVDGSTTFNSATVAAVGE 360

Query: 367 GFLARDITFENTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINCLVAG 426
           GFLARDITF+NTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINCL++G
Sbjct: 361 GFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINCLISG 420

Query: 427 TVDFIFGNAAAVFQDCDIHARKPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATSDLRP 486
           TVDFIFGNAAAVFQDCDIHAR+PNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATSDLR 
Sbjct: 421 TVDFIFGNAAAVFQDCDIHARRPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATSDLRS 480

Query: 487 VQKNFPTFLGRPWKEYSRTVIMQSSISDVIDPKGWHEWSGNFALNTLFYGEYQNTGPGAS 546
           VQK+FPTFLGRPWKEYSRTVIMQSSISDVID KGWHEWSGNFALNTLFYGEYQNTGPGAS
Sbjct: 481 VQKSFPTFLGRPWKEYSRTVIMQSSISDVIDSKGWHEWSGNFALNTLFYGEYQNTGPGAS 540

Query: 547 TGGRVTWKGFRIIRSATEAEGFTAGKFIGGGSWLRATGFPFSLGL 591
           T GRVTWKGFR+IRSATEAEGFT GKFIGGGSWLRATGFPFSLGL
Sbjct: 541 TSGRVTWKGFRVIRSATEAEGFTPGKFIGGGSWLRATGFPFSLGL 581

BLAST of Tan0019495 vs. NCBI nr
Match: XP_004141734.1 (pectinesterase [Cucumis sativus] >KGN45437.1 hypothetical protein Csa_016439 [Cucumis sativus])

HSP 1 Score: 1010.0 bits (2610), Expect = 8.5e-291
Identity = 518/598 (86.62%), Postives = 558/598 (93.31%), Query Frame = 0

Query: 1   MKETDNMEKPIA--AGISNSGE-QKTKKPNKNKKLIILALGSTLLLVGAVVGIVAGVSNS 60
           MKETD ME+PIA  AG+SNS + QKT+K +K KKL+I+ L STLL+VGAV+GIVAG   +
Sbjct: 1   MKETDAMEQPIAAGAGVSNSSDLQKTQKSSK-KKLLIVTLCSTLLIVGAVIGIVAG--TT 60

Query: 61  KTSGDRPSEAHAILKSTCSSTLYPDLCFSSISENEGALKKVTNQKEVIELSINITVKAVE 120
           K+S     E+HAI+KSTCSSTLYPD+C+S+IS +EGA+ KV NQK+VIE+SINITVKAVE
Sbjct: 61  KSSSKYSGESHAIVKSTCSSTLYPDMCYSAISRSEGAMLKVKNQKDVIEISINITVKAVE 120

Query: 121 ENYFRVQKLAALKNITHREKIALHDCLETIDETLDELHKAIVDLHEYPNKKSLAQHADDL 180
           ENYFRV+KL  LKNI+HRE+IALHDCLETIDETLDELHKAIVDL+EYPNKKSL QHADDL
Sbjct: 121 ENYFRVKKLCGLKNISHRERIALHDCLETIDETLDELHKAIVDLNEYPNKKSLNQHADDL 180

Query: 181 KTLLSSAITNQETCLDGFSHDEADKNLRDKLKEGQTHVEHMCSNALAMIKNMTDTDIANY 240
           KTLLSSAITNQETCLDGFSHD+ADKNLRDKLKEGQ  VEHMCSNALAMIKNMTDTDIANY
Sbjct: 181 KTLLSSAITNQETCLDGFSHDDADKNLRDKLKEGQMEVEHMCSNALAMIKNMTDTDIANY 240

Query: 241 EAKMG-TANRKLKEENDDH----GIQWPEWLSTGDRRLLQSSSVTPDVVVAADGSGNFKT 300
           EAKMG T NRKL  E DD+    GIQWPEWLS GDRRLLQSSSVTP+VVVAADGSGNF+T
Sbjct: 241 EAKMGITKNRKLMAEEDDNNNDDGIQWPEWLSAGDRRLLQSSSVTPNVVVAADGSGNFRT 300

Query: 301 VSAAVAAAPVKSSKRYVIRIKAGVYRENVEVPKKKTNIMFMGDGRSNTIITGSRNVVDGS 360
           V+AAVAAAPV+SSKRYVIRIKAGVYRENVEVPKKKTNIMF+GDGR NTIITGSRNVVDGS
Sbjct: 301 VAAAVAAAPVRSSKRYVIRIKAGVYRENVEVPKKKTNIMFIGDGRRNTIITGSRNVVDGS 360

Query: 361 TTFNSATVAAVGEGFLARDITFENTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVH 420
           TTFNSAT+AAVGEGFLARDITF+NTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVH
Sbjct: 361 TTFNSATMAAVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVH 420

Query: 421 SNRQFYINCLVAGTVDFIFGNAAAVFQDCDIHARKPNSGQKNMVTAQGRSDPNQNTGIVI 480
           SNRQFYINCLV+GTVDFIFGNAAA+FQDCDIHARKPNSGQKNMVTAQGRSDPNQNTGIVI
Sbjct: 421 SNRQFYINCLVSGTVDFIFGNAAAIFQDCDIHARKPNSGQKNMVTAQGRSDPNQNTGIVI 480

Query: 481 QKSRIGATSDLRPVQKNFPTFLGRPWKEYSRTVIMQSSISDVIDPKGWHEWSGNFALNTL 540
           QKSRIGATSDLRPVQK+FPTFLGRPWKEYSRTVIMQ +ISDVIDPKGWHEWSG+FALNTL
Sbjct: 481 QKSRIGATSDLRPVQKSFPTFLGRPWKEYSRTVIMQCTISDVIDPKGWHEWSGSFALNTL 540

Query: 541 FYGEYQNTGPGASTGGRVTWKGFRIIRSATEAEGFTAGKFIGGGSWLRATGFPFSLGL 591
           FYGEYQNTG GASTGGRVTWKGFR+IRSATEAE FTAGKFIGGGSWL +TGFPFSLGL
Sbjct: 541 FYGEYQNTGAGASTGGRVTWKGFRVIRSATEAESFTAGKFIGGGSWLSSTGFPFSLGL 595

BLAST of Tan0019495 vs. NCBI nr
Match: XP_023549002.1 (pectinesterase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1007.3 bits (2603), Expect = 5.5e-290
Identity = 519/588 (88.27%), Postives = 543/588 (92.35%), Query Frame = 0

Query: 7   MEKPIAAGISNSGEQKTKKPNKNKKLIILALGSTLLLVGAVVGIVAGVSNSKTSGDRPSE 66
           ME+ IAAGIS+SG Q  K  N  KKLIILA+ STLLL    VGIVAGV +S+  G    E
Sbjct: 1   MEQIIAAGISSSGNQLPKPKNNRKKLIILAVSSTLLL----VGIVAGVVSSRNGGGEEEE 60

Query: 67  A--HAILKSTCSSTLYPDLCFSSISENEGALKKVTNQKEVIELSINITVKAVEENYFRVQ 126
           A  HAI+KSTCSSTLYPDLCFSSI  ++GA+K+V N+K+VIE+SINITVKAVEENYFRV 
Sbjct: 61  AKSHAIVKSTCSSTLYPDLCFSSILRSDGAMKRVGNKKDVIEVSINITVKAVEENYFRVL 120

Query: 127 KLAALKNITHREKIALHDCLETIDETLDELHKAIVDLHEYPNKKSLAQHADDLKTLLSSA 186
           KL  LK+I+HREKIALHDCLETIDETLDELHKA VDLHEYPNKKSL Q ADDLKTLLSSA
Sbjct: 121 KLCELKDISHREKIALHDCLETIDETLDELHKATVDLHEYPNKKSLNQRADDLKTLLSSA 180

Query: 187 ITNQETCLDGFSHDEADKNLRDKLKEGQTHVEHMCSNALAMIKNMTDTDIANYEAKMG-- 246
           ITNQETCLDGFSHDEADKNLRDKLKEGQ  VE MCSNALAMIKNMTDTDIANYEAKMG  
Sbjct: 181 ITNQETCLDGFSHDEADKNLRDKLKEGQIEVERMCSNALAMIKNMTDTDIANYEAKMGTT 240

Query: 247 TANRKLKEENDDHGIQWPEWLSTGDRRLLQSSSVTPDVVVAADGSGNFKTVSAAVAAAPV 306
           TANRKLKEEN+D  IQWPEWLS GDRRLLQSSSV  DVVVAADGSGNFKTV+AAVAAAPV
Sbjct: 241 TANRKLKEENNDEQIQWPEWLSAGDRRLLQSSSVKADVVVAADGSGNFKTVAAAVAAAPV 300

Query: 307 KSSKRYVIRIKAGVYRENVEVPKKKTNIMFMGDGRSNTIITGSRNVVDGSTTFNSATVAA 366
           KSSKRYVIRIKAGVYRENV+VPKKKTNIMFMGDGRSNTIITGSRNVVDGSTTFNSATVAA
Sbjct: 301 KSSKRYVIRIKAGVYRENVDVPKKKTNIMFMGDGRSNTIITGSRNVVDGSTTFNSATVAA 360

Query: 367 VGEGFLARDITFENTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINCL 426
           VGEGFLARDITF+NTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINCL
Sbjct: 361 VGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINCL 420

Query: 427 VAGTVDFIFGNAAAVFQDCDIHARKPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATSD 486
           ++GTVDFIFGNAAAVFQDCDIHAR+PNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATSD
Sbjct: 421 ISGTVDFIFGNAAAVFQDCDIHARRPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATSD 480

Query: 487 LRPVQKNFPTFLGRPWKEYSRTVIMQSSISDVIDPKGWHEWSGNFALNTLFYGEYQNTGP 546
           LR VQK+FPTFLGRPWKEYSRTVIMQSSISDVID KGWHEWSGNFALNTLFYGEYQNTGP
Sbjct: 481 LRSVQKSFPTFLGRPWKEYSRTVIMQSSISDVIDSKGWHEWSGNFALNTLFYGEYQNTGP 540

Query: 547 GASTGGRVTWKGFRIIRSATEAEGFTAGKFIGGGSWLRATGFPFSLGL 591
           GAST GRVTWKGFR+IRSATEAEGFT GKFIGGGSWLRATGFPFSLGL
Sbjct: 541 GASTSGRVTWKGFRVIRSATEAEGFTPGKFIGGGSWLRATGFPFSLGL 584

BLAST of Tan0019495 vs. ExPASy TrEMBL
Match: A0A6J1JSY6 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111488604 PE=3 SV=1)

HSP 1 Score: 1013.8 bits (2620), Expect = 2.8e-292
Identity = 518/585 (88.55%), Postives = 545/585 (93.16%), Query Frame = 0

Query: 7   MEKPIAAGISNSGEQKTKKPNKNKKLIILALGSTLLLVGAVVGIVAGVSNSKTSGDRPSE 66
           ME+PIAAGIS+SG    K     KKLIILA+ STLLL    VGIVAGV +S+ +G   S+
Sbjct: 1   MEQPIAAGISSSGNHLPKPKYNRKKLIILAVSSTLLL----VGIVAGVVSSRNAGGEESK 60

Query: 67  AHAILKSTCSSTLYPDLCFSSISENEGALKKVTNQKEVIELSINITVKAVEENYFRVQKL 126
           +HAI+KSTCSSTLYPDLCFSSI +N+ A+KKV+N+K+VIE+SINITVKAVEENYF+V KL
Sbjct: 61  SHAIVKSTCSSTLYPDLCFSSILKNDEAMKKVSNKKDVIEVSINITVKAVEENYFKVLKL 120

Query: 127 AALKNITHREKIALHDCLETIDETLDELHKAIVDLHEYPNKKSLAQHADDLKTLLSSAIT 186
             LKNI+HREKIALHDCLETIDETLDELHKA VDLHEYPNKKSL Q ADDLKTLLSSAIT
Sbjct: 121 YELKNISHREKIALHDCLETIDETLDELHKATVDLHEYPNKKSLTQQADDLKTLLSSAIT 180

Query: 187 NQETCLDGFSHDEADKNLRDKLKEGQTHVEHMCSNALAMIKNMTDTDIANYEAKMGT-AN 246
           NQETCLDGFSHDEADKNLRDKLKEGQ  VE MCSNALAMIKNMTDTDIANYE KMGT AN
Sbjct: 181 NQETCLDGFSHDEADKNLRDKLKEGQIEVERMCSNALAMIKNMTDTDIANYETKMGTAAN 240

Query: 247 RKLKEENDDHGIQWPEWLSTGDRRLLQSSSVTPDVVVAADGSGNFKTVSAAVAAAPVKSS 306
           RKLKEEN+D  IQWPEWLS GDRRLLQSSSV PDVVVAADGSGNFKTV+AAVAAAPVKSS
Sbjct: 241 RKLKEENNDEQIQWPEWLSAGDRRLLQSSSVKPDVVVAADGSGNFKTVAAAVAAAPVKSS 300

Query: 307 KRYVIRIKAGVYRENVEVPKKKTNIMFMGDGRSNTIITGSRNVVDGSTTFNSATVAAVGE 366
           KRYVIRIKAGVYRENV+V KKKTNIMFMGDGRSNTIITGSRNVVDGSTTFNSATVAAVGE
Sbjct: 301 KRYVIRIKAGVYRENVDVQKKKTNIMFMGDGRSNTIITGSRNVVDGSTTFNSATVAAVGE 360

Query: 367 GFLARDITFENTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINCLVAG 426
           GFLARDITF+NTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINCL++G
Sbjct: 361 GFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINCLISG 420

Query: 427 TVDFIFGNAAAVFQDCDIHARKPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATSDLRP 486
           TVDFIFGNAAAVFQDCDIHAR+PNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATSDLR 
Sbjct: 421 TVDFIFGNAAAVFQDCDIHARRPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATSDLRS 480

Query: 487 VQKNFPTFLGRPWKEYSRTVIMQSSISDVIDPKGWHEWSGNFALNTLFYGEYQNTGPGAS 546
           VQK+FPTFLGRPWKEYSRTVIMQSSISDVID KGWHEWSGNFALNTLFYGEYQNTGPGAS
Sbjct: 481 VQKSFPTFLGRPWKEYSRTVIMQSSISDVIDSKGWHEWSGNFALNTLFYGEYQNTGPGAS 540

Query: 547 TGGRVTWKGFRIIRSATEAEGFTAGKFIGGGSWLRATGFPFSLGL 591
           TGGRVTWKGFR+IRSATEAEGFT GKFIGGGSWLRATGFPFSLGL
Sbjct: 541 TGGRVTWKGFRVIRSATEAEGFTPGKFIGGGSWLRATGFPFSLGL 581

BLAST of Tan0019495 vs. ExPASy TrEMBL
Match: A0A6J1GQR0 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111456237 PE=3 SV=1)

HSP 1 Score: 1011.9 bits (2615), Expect = 1.1e-291
Identity = 516/585 (88.21%), Postives = 546/585 (93.33%), Query Frame = 0

Query: 7   MEKPIAAGISNSGEQKTKKPNKNKKLIILALGSTLLLVGAVVGIVAGVSNSKTSGDRPSE 66
           ME+PIAAGIS+SG    K  N  KKLIILA+ STLLL    VGIVAGV +S+  G   ++
Sbjct: 1   MEQPIAAGISSSGNHLPKPKNNRKKLIILAVSSTLLL----VGIVAGVVSSRNGGGEEAK 60

Query: 67  AHAILKSTCSSTLYPDLCFSSISENEGALKKVTNQKEVIELSINITVKAVEENYFRVQKL 126
           +HAI+KSTCSSTLYPDLCFSSI +++GA+K+V ++K+VIE+SINITVKAVEENYF+V KL
Sbjct: 61  SHAIVKSTCSSTLYPDLCFSSILKSDGAMKRVGSKKDVIEVSINITVKAVEENYFKVLKL 120

Query: 127 AALKNITHREKIALHDCLETIDETLDELHKAIVDLHEYPNKKSLAQHADDLKTLLSSAIT 186
             LKNI+HREKIALHDCLETIDETLDELHKA VDLHEYPNKKSL QHADDLKTLLSSAIT
Sbjct: 121 YELKNISHREKIALHDCLETIDETLDELHKATVDLHEYPNKKSLNQHADDLKTLLSSAIT 180

Query: 187 NQETCLDGFSHDEADKNLRDKLKEGQTHVEHMCSNALAMIKNMTDTDIANYEAKMG-TAN 246
           NQETCLDGFSHDEADKNLRDKLKEGQ  VE MCSNALAMIKNMTDTDIANYEAKMG TAN
Sbjct: 181 NQETCLDGFSHDEADKNLRDKLKEGQIEVERMCSNALAMIKNMTDTDIANYEAKMGTTAN 240

Query: 247 RKLKEENDDHGIQWPEWLSTGDRRLLQSSSVTPDVVVAADGSGNFKTVSAAVAAAPVKSS 306
           RKLKEE +D  IQWPEWLS GDRRLLQSSSV PDVVVAADGSGNFKTV+AAVAAAPVKSS
Sbjct: 241 RKLKEEINDEQIQWPEWLSAGDRRLLQSSSVKPDVVVAADGSGNFKTVAAAVAAAPVKSS 300

Query: 307 KRYVIRIKAGVYRENVEVPKKKTNIMFMGDGRSNTIITGSRNVVDGSTTFNSATVAAVGE 366
           KR+VIRIKAGVYRENV+VPKKKTNIMFMGDGRSNTIITGSRNVVDGSTTFNSATVAAVGE
Sbjct: 301 KRFVIRIKAGVYRENVDVPKKKTNIMFMGDGRSNTIITGSRNVVDGSTTFNSATVAAVGE 360

Query: 367 GFLARDITFENTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINCLVAG 426
           GFLARDITF+NTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINCL++G
Sbjct: 361 GFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINCLISG 420

Query: 427 TVDFIFGNAAAVFQDCDIHARKPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATSDLRP 486
           TVDFIFGNAAAVFQDCDIHAR+PNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATSDLR 
Sbjct: 421 TVDFIFGNAAAVFQDCDIHARRPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATSDLRS 480

Query: 487 VQKNFPTFLGRPWKEYSRTVIMQSSISDVIDPKGWHEWSGNFALNTLFYGEYQNTGPGAS 546
           VQK+FPTFLGRPWKEYSRTVIMQSSISDVID KGWHEWSGNFALNTLFYGEYQNTGPGAS
Sbjct: 481 VQKSFPTFLGRPWKEYSRTVIMQSSISDVIDSKGWHEWSGNFALNTLFYGEYQNTGPGAS 540

Query: 547 TGGRVTWKGFRIIRSATEAEGFTAGKFIGGGSWLRATGFPFSLGL 591
           T GRVTWKGFR+IRSATEAEGFT GKFIGGGSWLRATGFPFSLGL
Sbjct: 541 TSGRVTWKGFRVIRSATEAEGFTPGKFIGGGSWLRATGFPFSLGL 581

BLAST of Tan0019495 vs. ExPASy TrEMBL
Match: A0A0A0KAM6 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_7G447990 PE=3 SV=1)

HSP 1 Score: 1010.0 bits (2610), Expect = 4.1e-291
Identity = 518/598 (86.62%), Postives = 558/598 (93.31%), Query Frame = 0

Query: 1   MKETDNMEKPIA--AGISNSGE-QKTKKPNKNKKLIILALGSTLLLVGAVVGIVAGVSNS 60
           MKETD ME+PIA  AG+SNS + QKT+K +K KKL+I+ L STLL+VGAV+GIVAG   +
Sbjct: 1   MKETDAMEQPIAAGAGVSNSSDLQKTQKSSK-KKLLIVTLCSTLLIVGAVIGIVAG--TT 60

Query: 61  KTSGDRPSEAHAILKSTCSSTLYPDLCFSSISENEGALKKVTNQKEVIELSINITVKAVE 120
           K+S     E+HAI+KSTCSSTLYPD+C+S+IS +EGA+ KV NQK+VIE+SINITVKAVE
Sbjct: 61  KSSSKYSGESHAIVKSTCSSTLYPDMCYSAISRSEGAMLKVKNQKDVIEISINITVKAVE 120

Query: 121 ENYFRVQKLAALKNITHREKIALHDCLETIDETLDELHKAIVDLHEYPNKKSLAQHADDL 180
           ENYFRV+KL  LKNI+HRE+IALHDCLETIDETLDELHKAIVDL+EYPNKKSL QHADDL
Sbjct: 121 ENYFRVKKLCGLKNISHRERIALHDCLETIDETLDELHKAIVDLNEYPNKKSLNQHADDL 180

Query: 181 KTLLSSAITNQETCLDGFSHDEADKNLRDKLKEGQTHVEHMCSNALAMIKNMTDTDIANY 240
           KTLLSSAITNQETCLDGFSHD+ADKNLRDKLKEGQ  VEHMCSNALAMIKNMTDTDIANY
Sbjct: 181 KTLLSSAITNQETCLDGFSHDDADKNLRDKLKEGQMEVEHMCSNALAMIKNMTDTDIANY 240

Query: 241 EAKMG-TANRKLKEENDDH----GIQWPEWLSTGDRRLLQSSSVTPDVVVAADGSGNFKT 300
           EAKMG T NRKL  E DD+    GIQWPEWLS GDRRLLQSSSVTP+VVVAADGSGNF+T
Sbjct: 241 EAKMGITKNRKLMAEEDDNNNDDGIQWPEWLSAGDRRLLQSSSVTPNVVVAADGSGNFRT 300

Query: 301 VSAAVAAAPVKSSKRYVIRIKAGVYRENVEVPKKKTNIMFMGDGRSNTIITGSRNVVDGS 360
           V+AAVAAAPV+SSKRYVIRIKAGVYRENVEVPKKKTNIMF+GDGR NTIITGSRNVVDGS
Sbjct: 301 VAAAVAAAPVRSSKRYVIRIKAGVYRENVEVPKKKTNIMFIGDGRRNTIITGSRNVVDGS 360

Query: 361 TTFNSATVAAVGEGFLARDITFENTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVH 420
           TTFNSAT+AAVGEGFLARDITF+NTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVH
Sbjct: 361 TTFNSATMAAVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVH 420

Query: 421 SNRQFYINCLVAGTVDFIFGNAAAVFQDCDIHARKPNSGQKNMVTAQGRSDPNQNTGIVI 480
           SNRQFYINCLV+GTVDFIFGNAAA+FQDCDIHARKPNSGQKNMVTAQGRSDPNQNTGIVI
Sbjct: 421 SNRQFYINCLVSGTVDFIFGNAAAIFQDCDIHARKPNSGQKNMVTAQGRSDPNQNTGIVI 480

Query: 481 QKSRIGATSDLRPVQKNFPTFLGRPWKEYSRTVIMQSSISDVIDPKGWHEWSGNFALNTL 540
           QKSRIGATSDLRPVQK+FPTFLGRPWKEYSRTVIMQ +ISDVIDPKGWHEWSG+FALNTL
Sbjct: 481 QKSRIGATSDLRPVQKSFPTFLGRPWKEYSRTVIMQCTISDVIDPKGWHEWSGSFALNTL 540

Query: 541 FYGEYQNTGPGASTGGRVTWKGFRIIRSATEAEGFTAGKFIGGGSWLRATGFPFSLGL 591
           FYGEYQNTG GASTGGRVTWKGFR+IRSATEAE FTAGKFIGGGSWL +TGFPFSLGL
Sbjct: 541 FYGEYQNTGAGASTGGRVTWKGFRVIRSATEAESFTAGKFIGGGSWLSSTGFPFSLGL 595

BLAST of Tan0019495 vs. ExPASy TrEMBL
Match: A0A5A7V0J0 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold347G001680 PE=3 SV=1)

HSP 1 Score: 1005.7 bits (2599), Expect = 7.7e-290
Identity = 519/598 (86.79%), Postives = 554/598 (92.64%), Query Frame = 0

Query: 1   MKETDNMEKPIA--AGISNSGEQKTKKPNKNKKLIILALGSTLLLVGAVVGIVAGVS-NS 60
           MKETD ME+PIA  AGISNS + K K    +KKL+IL L STLL+VGAV+GIVAG + +S
Sbjct: 1   MKETDAMEQPIAAGAGISNSSDLK-KNKKASKKLLILTLSSTLLIVGAVIGIVAGTTKSS 60

Query: 61  KTSGDRPSEAHAILKSTCSSTLYPDLCFSSISENEGALKKVTNQKEVIELSINITVKAVE 120
           K SG    ++HAI+KSTCSSTLYPD+C+S+IS++EGA+ KV +QK+VIE+SINITVKAVE
Sbjct: 61  KDSG----KSHAIVKSTCSSTLYPDMCYSAISKSEGAMLKVKSQKDVIEVSINITVKAVE 120

Query: 121 ENYFRVQKLAALKNITHREKIALHDCLETIDETLDELHKAIVDLHEYPNKKSLAQHADDL 180
           ENYFRV+KL  LKNI+HREKIALHDCLETIDETLDELHKAIVDL+EYPNKKSL QHADDL
Sbjct: 121 ENYFRVKKLCGLKNISHREKIALHDCLETIDETLDELHKAIVDLNEYPNKKSLNQHADDL 180

Query: 181 KTLLSSAITNQETCLDGFSHDEADKNLRDKLKEGQTHVEHMCSNALAMIKNMTDTDIANY 240
           KTLLSSAITNQETCLDGFSHD ADK LRDKLK GQ  VEHMCSNALAMIKNMTDTDIANY
Sbjct: 181 KTLLSSAITNQETCLDGFSHDNADKTLRDKLKAGQMEVEHMCSNALAMIKNMTDTDIANY 240

Query: 241 EAKMG-TANRKLKEENDDHG----IQWPEWLSTGDRRLLQSSSVTPDVVVAADGSGNFKT 300
           EAKMG T NRKL  E +D+     IQWPEWLS GDRRLLQSSSVTP+VVVAADGSGNF+T
Sbjct: 241 EAKMGITKNRKLMAEEEDNNNNDEIQWPEWLSAGDRRLLQSSSVTPNVVVAADGSGNFRT 300

Query: 301 VSAAVAAAPVKSSKRYVIRIKAGVYRENVEVPKKKTNIMFMGDGRSNTIITGSRNVVDGS 360
           V+AAVAAAP KSSKRYVIRIKAGVYRENVEVPKKKTNIMFMGDGR NTIITGSRNVVDGS
Sbjct: 301 VAAAVAAAPEKSSKRYVIRIKAGVYRENVEVPKKKTNIMFMGDGRRNTIITGSRNVVDGS 360

Query: 361 TTFNSATVAAVGEGFLARDITFENTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVH 420
           TTF SATVAAVGEGFLARDITF+NTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVH
Sbjct: 361 TTFKSATVAAVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVH 420

Query: 421 SNRQFYINCLVAGTVDFIFGNAAAVFQDCDIHARKPNSGQKNMVTAQGRSDPNQNTGIVI 480
           SNRQFYINCLV+GTVDFIFGNAAA+FQDCDIHARKPNSGQKNMVTAQGRSDPNQNTGIVI
Sbjct: 421 SNRQFYINCLVSGTVDFIFGNAAAIFQDCDIHARKPNSGQKNMVTAQGRSDPNQNTGIVI 480

Query: 481 QKSRIGATSDLRPVQKNFPTFLGRPWKEYSRTVIMQSSISDVIDPKGWHEWSGNFALNTL 540
           QKSRIGATSDLRPVQK+FPTFLGRPWKEYSRTVIMQ SISDVIDPKGWHEWSG+FALNTL
Sbjct: 481 QKSRIGATSDLRPVQKSFPTFLGRPWKEYSRTVIMQCSISDVIDPKGWHEWSGSFALNTL 540

Query: 541 FYGEYQNTGPGASTGGRVTWKGFRIIRSATEAEGFTAGKFIGGGSWLRATGFPFSLGL 591
           FYGEYQNTGPGASTGGRVTWKGFR+IRSATEAEGFTAGKFIGGGSWLR+TGFPFSLGL
Sbjct: 541 FYGEYQNTGPGASTGGRVTWKGFRVIRSATEAEGFTAGKFIGGGSWLRSTGFPFSLGL 593

BLAST of Tan0019495 vs. ExPASy TrEMBL
Match: A0A6J1DGG5 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111020634 PE=3 SV=1)

HSP 1 Score: 1001.5 bits (2588), Expect = 1.5e-288
Identity = 513/590 (86.95%), Postives = 548/590 (92.88%), Query Frame = 0

Query: 7   MEKPIAAG--ISNSGEQKTKKP-NKN-KKLIILALGSTLLLVGAVVGIVAGVSNSKTSGD 66
           MEKPI AG  IS+SG + T  P N+N KK ++LAL + LL+VGAVVGIVAGV+ S+ SG 
Sbjct: 1   MEKPIVAGMEISDSGHRSTPVPRNRNSKKTLLLALSAALLVVGAVVGIVAGVTKSRNSGR 60

Query: 67  RPSEAHAILKSTCSSTLYPDLCFSSISENEGALKKVTNQKEVIELSINITVKAVEENYFR 126
           +  EAHAI+KSTCS+TLYP+LCFS+IS +EGA+KKV+NQK+VIELSINITVKAVEENYFR
Sbjct: 61  QSEEAHAIVKSTCSATLYPELCFSTISRSEGAMKKVSNQKDVIELSINITVKAVEENYFR 120

Query: 127 VQKLAALKNITHREKIALHDCLETIDETLDELHKAIVDLHEYPNKKSLAQHADDLKTLLS 186
           VQKLAALKNITHRE+IALHDCLETIDETLDELHK+IVDLH+YPNKKSL QHADDLKTLLS
Sbjct: 121 VQKLAALKNITHRERIALHDCLETIDETLDELHKSIVDLHQYPNKKSLDQHADDLKTLLS 180

Query: 187 SAITNQETCLDGFSHDEADKNLRDKLKEGQTHVEHMCSNALAMIKNMTDTDIANYEAKMG 246
           SAITNQETCLDGFSHD+ADK+LR KL+EGQ  VEHMCSNALAMIKNMTDTDIANY AKMG
Sbjct: 181 SAITNQETCLDGFSHDDADKSLRKKLEEGQKEVEHMCSNALAMIKNMTDTDIANYHAKMG 240

Query: 247 TANRKLKEEND--DHGIQWPEWLSTGDRRLLQSSSVTPDVVVAADGSGNFKTVSAAVAAA 306
            ANR L E  D  D  I WP WLS GDRRLLQSSSVTPD VVAADGSGNF+TVSAAV+AA
Sbjct: 241 -ANRNLIENADAGDAAIIWPHWLSPGDRRLLQSSSVTPDAVVAADGSGNFRTVSAAVSAA 300

Query: 307 PVKSSKRYVIRIKAGVYRENVEVPKKKTNIMFMGDGRSNTIITGSRNVVDGSTTFNSATV 366
           P KS KRY+IRIKAGVYRENVE+PKKKTNIMF+GDGRSNTIITGSRNVVDGSTTFNSATV
Sbjct: 301 PDKSKKRYIIRIKAGVYRENVEIPKKKTNIMFVGDGRSNTIITGSRNVVDGSTTFNSATV 360

Query: 367 AAVGEGFLARDITFENTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYIN 426
           AAVGEGFLARDITF+NTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYIN
Sbjct: 361 AAVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYIN 420

Query: 427 CLVAGTVDFIFGNAAAVFQDCDIHARKPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGAT 486
           CLV+GTVDFIFGNAAAVFQDCDIHAR+PNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGAT
Sbjct: 421 CLVSGTVDFIFGNAAAVFQDCDIHARRPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGAT 480

Query: 487 SDLRPVQKNFPTFLGRPWKEYSRTVIMQSSISDVIDPKGWHEWSGNFALNTLFYGEYQNT 546
           SDLR VQKNFPTFLGRPWKEYSRTVIMQSSISDVIDPKGWHEWSGNFALNTLFY EYQNT
Sbjct: 481 SDLRSVQKNFPTFLGRPWKEYSRTVIMQSSISDVIDPKGWHEWSGNFALNTLFYAEYQNT 540

Query: 547 GPGASTGGRVTWKGFRIIRSATEAEGFTAGKFIGGGSWLRATGFPFSLGL 591
           GPGAST GRVTWKGF++IRSATEAE FTAG+FIGGGSWLRATGFPFSLGL
Sbjct: 541 GPGASTAGRVTWKGFKVIRSATEAEPFTAGRFIGGGSWLRATGFPFSLGL 589

BLAST of Tan0019495 vs. TAIR 10
Match: AT3G14310.1 (pectin methylesterase 3 )

HSP 1 Score: 799.7 bits (2064), Expect = 1.6e-231
Identity = 408/579 (70.47%), Postives = 468/579 (80.83%), Query Frame = 0

Query: 28  KNKKLIILALGSTLLLVGAVVGIVAGVSNSKTSGDRPSEAHAILKSTCSSTLYPDLCFSS 87
           KNKKL++L+    LL V AV GI AG S +         +HA+L+S+CSST YP+LC S+
Sbjct: 16  KNKKLVLLSAAVALLFVAAVAGISAGASKANEKRTLSPSSHAVLRSSCSSTRYPELCISA 75

Query: 88  ISENEGALKKVTNQKEVIELSINITVKAVEENYFRVQKL-AALKNITHREKIALHDCLET 147
           +    G   ++T+QK+VIE S+N+T+ AVE NYF V+KL    K +T REK ALHDCLET
Sbjct: 76  VVTAGGV--ELTSQKDVIEASVNLTITAVEHNYFTVKKLIKKRKGLTPREKTALHDCLET 135

Query: 148 IDETLDELHKAIVDLHEYPNKKSLAQHADDLKTLLSSAITNQETCLDGFSHDEADKNLRD 207
           IDETLDELH+ + DLH YP KK+L +HA DLKTL+SSAITNQETCLDGFSHD+ADK +R 
Sbjct: 136 IDETLDELHETVEDLHLYPTKKTLREHAGDLKTLISSAITNQETCLDGFSHDDADKQVRK 195

Query: 208 KLKEGQTHVEHMCSNALAMIKNMTDTDIANYE--AKMGTANRKLKEENDDHGIQ------ 267
            L +GQ HVEHMCSNALAMIKNMTDTDIAN+E  AK+ + NRKLKEEN +  +       
Sbjct: 196 ALLKGQIHVEHMCSNALAMIKNMTDTDIANFEQKAKITSNNRKLKEENQETTVAVDIAGA 255

Query: 268 -------WPEWLSTGDRRLLQSSSVTPDVVVAADGSGNFKTVSAAVAAAPVKSSKRYVIR 327
                  WP WLS GDRRLLQ S V  D  VAADGSG FKTV+AAVAAAP  S+KRYVI 
Sbjct: 256 GELDSEGWPTWLSAGDRRLLQGSGVKADATVAADGSGTFKTVAAAVAAAPENSNKRYVIH 315

Query: 328 IKAGVYRENVEVPKKKTNIMFMGDGRSNTIITGSRNVVDGSTTFNSATVAAVGEGFLARD 387
           IKAGVYRENVEV KKK NIMFMGDGR+ TIITGSRNVVDGSTTF+SATVAAVGE FLARD
Sbjct: 316 IKAGVYRENVEVAKKKKNIMFMGDGRTRTIITGSRNVVDGSTTFHSATVAAVGERFLARD 375

Query: 388 ITFENTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINCLVAGTVDFIF 447
           ITF+NTAGPSKHQAVALRVG+D SAFY CDMLAYQDTLYVHSNRQF++ CL+AGTVDFIF
Sbjct: 376 ITFQNTAGPSKHQAVALRVGSDFSAFYNCDMLAYQDTLYVHSNRQFFVKCLIAGTVDFIF 435

Query: 448 GNAAAVFQDCDIHARKPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATSDLRPVQKNFP 507
           GNAA V QDCDIHAR+PNSGQKNMVTAQGR+DPNQNTGIVIQK RIGATSDL+ V+ +FP
Sbjct: 436 GNAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATSDLQSVKGSFP 495

Query: 508 TFLGRPWKEYSRTVIMQSSISDVIDPKGWHEWSGNFALNTLFYGEYQNTGPGASTGGRVT 567
           T+LGRPWKEYS+TVIMQS+ISDVI P+GW EW+G FALNTL Y EY NTG GA T  RV 
Sbjct: 496 TYLGRPWKEYSQTVIMQSAISDVIRPEGWSEWTGTFALNTLTYREYSNTGAGAGTANRVK 555

Query: 568 WKGFRIIRSATEAEGFTAGKFIGGGSWLRATGFPFSLGL 591
           W+GF++I +A EA+ +TAG+FIGGG WL +TGFPFSLGL
Sbjct: 556 WRGFKVITAAAEAQKYTAGQFIGGGGWLSSTGFPFSLGL 592

BLAST of Tan0019495 vs. TAIR 10
Match: AT1G53830.1 (pectin methylesterase 2 )

HSP 1 Score: 780.8 bits (2015), Expect = 7.8e-226
Identity = 403/573 (70.33%), Postives = 463/573 (80.80%), Query Frame = 0

Query: 29  NKKLIILALGSTLLLVGAVVGIVAGVSNSKTS---GDRPSEAHAILKSTCSSTLYPDLCF 88
           NKKLI+ +    LLL+ ++VGI A  +N   +       S +HAILKS CSSTLYP+LCF
Sbjct: 17  NKKLILSSAAIALLLLASIVGIAATTTNQNKNQKITTLSSTSHAILKSVCSSTLYPELCF 76

Query: 89  SSISENEGALKKVTNQKEVIELSINITVKAVEENYFRVQKL-AALKNITHREKIALHDCL 148
           S+++   G  K++T+QKEVIE S+N+T KAV+ NYF V+KL A  K +T RE  ALHDCL
Sbjct: 77  SAVAATGG--KELTSQKEVIEASLNLTTKAVKHNYFAVKKLIAKRKGLTPREVTALHDCL 136

Query: 149 ETIDETLDELHKAIVDLHEYPNKKSLAQHADDLKTLLSSAITNQETCLDGFSHDEADKNL 208
           ETIDETLDELH A+ DLH+YP +KSL +HADDLKTL+SSAITNQ TCLDGFS+D+AD+ +
Sbjct: 137 ETIDETLDELHVAVEDLHQYPKQKSLRKHADDLKTLISSAITNQGTCLDGFSYDDADRKV 196

Query: 209 RDKLKEGQTHVEHMCSNALAMIKNMTDTDIANYEAK------MGTANRKLKEENDD-HGI 268
           R  L +GQ HVEHMCSNALAMIKNMT+TDIAN+E +          NRKLKE   D    
Sbjct: 197 RKALLKGQVHVEHMCSNALAMIKNMTETDIANFELRDKSSTFTNNNNRKLKEVTGDLDSD 256

Query: 269 QWPEWLSTGDRRLLQSSSVTPDVVVAADGSGNFKTVSAAVAAAPVKSSKRYVIRIKAGVY 328
            WP+WLS GDRRLLQ S++  D  VA DGSG+F TV+AAVAAAP KS+KR+VI IKAGVY
Sbjct: 257 GWPKWLSVGDRRLLQGSTIKADATVADDGSGDFTTVAAAVAAAPEKSNKRFVIHIKAGVY 316

Query: 329 RENVEVPKKKTNIMFMGDGRSNTIITGSRNVVDGSTTFNSATVAAVGEGFLARDITFENT 388
           RENVEV KKKTNIMF+GDGR  TIITGSRNVVDGSTTF+SATVAAVGE FLARDITF+NT
Sbjct: 317 RENVEVTKKKTNIMFLGDGRGKTIITGSRNVVDGSTTFHSATVAAVGERFLARDITFQNT 376

Query: 389 AGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINCLVAGTVDFIFGNAAAV 448
           AGPSKHQAVALRVG+D SAFYQCDM AYQDTLYVHSNRQF++ C + GTVDFIFGNAAAV
Sbjct: 377 AGPSKHQAVALRVGSDFSAFYQCDMFAYQDTLYVHSNRQFFVKCHITGTVDFIFGNAAAV 436

Query: 449 FQDCDIHARKPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATSDLRPVQKNFPTFLGRP 508
            QDCDI+AR+PNSGQKNMVTAQGRSDPNQNTGIVIQ  RIG TSDL  V+  FPT+LGRP
Sbjct: 437 LQDCDINARRPNSGQKNMVTAQGRSDPNQNTGIVIQNCRIGGTSDLLAVKGTFPTYLGRP 496

Query: 509 WKEYSRTVIMQSSISDVIDPKGWHEWSGNFALNTLFYGEYQNTGPGASTGGRVTWKGFRI 568
           WKEYSRTVIMQS ISDVI P+GWHEWSG+FAL+TL Y EY N G GA T  RV WKG+++
Sbjct: 497 WKEYSRTVIMQSDISDVIRPEGWHEWSGSFALDTLTYREYLNRGGGAGTANRVKWKGYKV 556

Query: 569 IRSATEAEGFTAGKFIGGGSWLRATGFPFSLGL 591
           I S TEA+ FTAG+FIGGG WL +TGFPFSL L
Sbjct: 557 ITSDTEAQPFTAGQFIGGGGWLASTGFPFSLSL 587

BLAST of Tan0019495 vs. TAIR 10
Match: AT5G53370.1 (pectin methylesterase PCR fragment F )

HSP 1 Score: 444.1 bits (1141), Expect = 1.7e-124
Identity = 254/590 (43.05%), Postives = 357/590 (60.51%), Query Frame = 0

Query: 17  NSGEQKTKKPNKNK----KLIILALGSTLLLVGAV-VGIVAG---VSNSKTSGDRPSEAH 76
           N  +  T  P   K    KLI+  L   +L+VG V  GI AG   V + KT      +  
Sbjct: 15  NQKDPATSLPELQKKTKTKLILFTL--AVLVVGVVCFGIFAGIRAVDSGKTEPKLTRKPT 74

Query: 77  AILKSTCSSTLYPDLCFSSISENEGALKKVTNQKEVIELSINITVKAVEENYFRVQKLAA 136
             +  TCS +LYP+LC  ++ +  G+L    ++ E+I +S N T++   +  +       
Sbjct: 75  QAISRTCSKSLYPNLCIDTLLDFPGSL--TADENELIHISFNATLQKFSKALY-TSSTIT 134

Query: 137 LKNITHREKIALHDCLETIDETLDELHKAIVDLHEYPNKKSLAQHADDLKTLLSSAITNQ 196
              +  R + A   CLE +D+++D L +A+  +      +S      D+ T LSSA+TN 
Sbjct: 135 YTQMPPRVRSAYDSCLELLDDSVDALTRALSSVVVVSGDES----HSDVMTWLSSAMTNH 194

Query: 197 ETCLDGFSHDEAD-KNLRDKLKEGQTHVEHMCSNALAM----IKNMTDTDIANYEAKMGT 256
           +TC DGF   E     ++D++      +  M SN LA+    +K+++   + N    +GT
Sbjct: 195 DTCTDGFDEIEGQGGEVKDQVIGAVKDLSEMVSNCLAIFAGKVKDLSGVPVVNNRKLLGT 254

Query: 257 ANRKLKEENDDHGIQWPEWLSTGDRRLL--QSSSVTPDVVVAADGSGNFKTVSAAVAAAP 316
                 EE +    + P WL   DR LL   +S++  D+ V+ DGSG FKT++ A+  AP
Sbjct: 255 ------EETE----ELPNWLKREDRELLGTPTSAIQADITVSKDGSGTFKTIAEAIKKAP 314

Query: 317 VKSSKRYVIRIKAGVY-RENVEVPKKKTNIMFMGDGRSNTIITGSRNVVDGSTTFNSATV 376
             SS+R+VI +KAG Y  EN++V +KKTN+MF+GDG+  T+ITG +++ D  TTF++AT 
Sbjct: 315 EHSSRRFVIYVKAGRYEEENLKVGRKKTNLMFIGDGKGKTVITGGKSIADDLTTFHTATF 374

Query: 377 AAVGEGFLARDITFENTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYIN 436
           AA G GF+ RD+TFEN AGP+KHQAVALRVG D +  Y+C+++ YQD LYVHSNRQF+  
Sbjct: 375 AATGAGFIVRDMTFENYAGPAKHQAVALRVGGDHAVVYRCNIIGYQDALYVHSNRQFFRE 434

Query: 437 CLVAGTVDFIFGNAAAVFQDCDIHARKPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGAT 496
           C + GTVDFIFGNAA + Q C+I+ARKP + QK  +TAQ R DPNQNTGI I   ++ AT
Sbjct: 435 CEIYGTVDFIFGNAAVILQSCNIYARKPMAQQKITITAQNRKDPNQNTGISIHACKLLAT 494

Query: 497 SDLRPVQKNFPTFLGRPWKEYSRTVIMQSSISDVIDPKGWHEWSGNFALNTLFYGEYQNT 556
            DL   + ++PT+LGRPWK YSR V M S + D IDP+GW EW+G FAL++L+YGEY N 
Sbjct: 495 PDLEASKGSYPTYLGRPWKLYSRVVYMMSDMGDHIDPRGWLEWNGPFALDSLYYGEYMNK 554

Query: 557 GPGASTGGRVTWKGFRIIRSATEAEGFTAGKFIGGGSWLRATGFPFSLGL 591
           G G+  G RV W G+ +I S  EA  FT  +FI G SWL +TG  F  GL
Sbjct: 555 GLGSGIGQRVKWPGYHVITSTVEASKFTVAQFISGSSWLPSTGVSFFSGL 585

BLAST of Tan0019495 vs. TAIR 10
Match: AT1G11580.1 (methylesterase PCR A )

HSP 1 Score: 441.0 bits (1133), Expect = 1.5e-123
Identity = 259/576 (44.97%), Postives = 353/576 (61.28%), Query Frame = 0

Query: 15  ISNSGEQKTKKPNKNKKLIILALGSTLLLVGAVVGIVAGVSNSKTSGDRPSEAHAILKST 74
           +SNS +    KP   K   +  + S + ++G+V    A + +  T+ +   ++       
Sbjct: 1   MSNSNQPLLSKPKSLKHKNLCLVLSFVAILGSVAFFTAQLISVNTNNN--DDSLLTTSQI 60

Query: 75  CSSTLYPDLCFSSISENEGALKKVTNQKEVIELSINITVKAVEENYFRVQKLAALKNITH 134
           C      D C + +SE         N+ +++ + +  +V  +E     V + A +++   
Sbjct: 61  CHGAHDQDSCQALLSEFTTLSLSKLNRLDLLHVFLKNSVWRLESTMTMVSE-ARIRSNGV 120

Query: 135 REKIALHDCLETIDETLDELHKAIVDLHEYPNKKSLAQHADDLKTLLSSAITNQETCLDG 194
           R+K    DC E +D + D +  ++ +L       +L  ++ ++ T LSS +TN  TCL+ 
Sbjct: 121 RDKAGFADCEEMMDVSKDRMMSSMEELR--GGNYNLESYS-NVHTWLSSVLTNYMTCLES 180

Query: 195 FSHDEADKNLRDKLKEGQTHVEHMCSNALAMIKNMTDTDIANYEAKMGTANRKLKEENDD 254
            S  +   N +  +K     +E + S A   +        A  + KM  +NR        
Sbjct: 181 IS--DVSVNSKQIVK---PQLEDLVSRARVALAIFVSVLPARDDLKMIISNR-------- 240

Query: 255 HGIQWPEWLSTGDRRLLQSS----SVTPDVVVAADGSGNFKTVSAAVAAAPVKSSKRYVI 314
               +P WL+  DR+LL+SS     VT +VVVA DG+G FKTV+ AVAAAP  S+ RYVI
Sbjct: 241 ----FPSWLTALDRKLLESSPKTLKVTANVVVAKDGTGKFKTVNEAVAAAPENSNTRYVI 300

Query: 315 RIKAGVYRENVEVPKKKTNIMFMGDGRSNTIITGSRNVVDGSTTFNSATVAAVGEGFLAR 374
            +K GVY+E +++ KKK N+M +GDG+  TIITGS NV+DGSTTF SATVAA G+GF+A+
Sbjct: 301 YVKKGVYKETIDIGKKKKNLMLVGDGKDATIITGSLNVIDGSTTFRSATVAANGDGFMAQ 360

Query: 375 DITFENTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINCLVAGTVDFI 434
           DI F+NTAGP+KHQAVALRV AD +   +C + AYQDTLY H+ RQFY +  + GTVDFI
Sbjct: 361 DIWFQNTAGPAKHQAVALRVSADQTVINRCRIDAYQDTLYTHTLRQFYRDSYITGTVDFI 420

Query: 435 FGNAAAVFQDCDIHARKPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATSDLRPVQKNF 494
           FGN+A VFQ+CDI AR P +GQKNM+TAQGR D NQNT I IQK +I A+SDL PV+ + 
Sbjct: 421 FGNSAVVFQNCDIVARNPGAGQKNMLTAQGREDQNQNTAISIQKCKITASSDLAPVKGSV 480

Query: 495 PTFLGRPWKEYSRTVIMQSSISDVIDPKGWHEWSGNFALNTLFYGEYQNTGPGASTGGRV 554
            TFLGRPWK YSRTVIMQS I + IDP GW  W G FAL+TL+YGEY NTGPGA T  RV
Sbjct: 481 KTFLGRPWKLYSRTVIMQSFIDNHIDPAGWFPWDGEFALSTLYYGEYANTGPGADTSKRV 540

Query: 555 TWKGFRIIRSATEAEGFTAGKFIGGGSWLRATGFPF 587
            WKGF++I+ + EAE FT  K I GG WL+ TG  F
Sbjct: 541 NWKGFKVIKDSKEAEQFTVAKLIQGGLWLKPTGVTF 553

BLAST of Tan0019495 vs. TAIR 10
Match: AT2G45220.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 439.9 bits (1130), Expect = 3.3e-123
Identity = 255/520 (49.04%), Postives = 329/520 (63.27%), Query Frame = 0

Query: 71  LKSTCSSTLYPDLCFSSISENEGALKKVTNQKEVIELSINITVKAVEENYFRVQKLAALK 130
           +K+ CS T  P  C   ++ N    + + ++ E +++S+ + +            L    
Sbjct: 30  VKAWCSQTPNPKPCEYFLTHNSNN-EPIKSESEFLKISMKLVLDRAILAKTHAFTLGPKC 89

Query: 131 NITHREKIALHDCLETIDETLDELHKAIVDLHEYPNKKSLAQHADDLKTLLSSAITNQET 190
             T REK A  DC++  D T+ ++++ +      PN K       D +T LS+A+TN +T
Sbjct: 90  RDT-REKAAWEDCIKLYDLTVSKINETM-----DPNVKCSKL---DAQTWLSTALTNLDT 149

Query: 191 CLDGFSHDEADKNLRDKLKEGQTHVEHMCSNALAMIKNMTDTDIANYEAKMGTANRKLKE 250
           C  GF            L+ G T +       L ++ N     + N  A           
Sbjct: 150 CRAGF------------LELGVTDI------VLPLMSNNVSNLLCNTLAINKVPFNYTPP 209

Query: 251 ENDDHGIQWPEWLSTGDRRLLQSSSVTPDVVVAADGSGNFKTVSAAVAAAPVKSSKRYVI 310
           E D     +P W+  GDR+LLQSS+   + VVA DGSGNFKT+  A+ AA    S R+VI
Sbjct: 210 EKDG----FPSWVKPGDRKLLQSSTPKDNAVVAKDGSGNFKTIKEAIDAA--SGSGRFVI 269

Query: 311 RIKAGVYRENVEVPKKKTNIMFMGDGRSNTIITGSRNVVDGSTTFNSATVAAVGEGFLAR 370
            +K GVY EN+E+ KK  N+M  GDG   TIITGS++V  G+TTFNSATVAAVG+GF+AR
Sbjct: 270 YVKQGVYSENLEIRKK--NVMLRGDGIGKTIITGSKSVGGGTTTFNSATVAAVGDGFIAR 329

Query: 371 DITFENTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINCLVAGTVDFI 430
            ITF NTAG S  QAVALR G+DLS FYQC   AYQDTLYVHSNRQFY +C V GTVDFI
Sbjct: 330 GITFRNTAGASNEQAVALRSGSDLSVFYQCSFEAYQDTLYVHSNRQFYRDCDVYGTVDFI 389

Query: 431 FGNAAAVFQDCDIHARKPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATSDLRPVQKNF 490
           FGNAAAV Q+C+I AR+P S + N +TAQGRSDPNQNTGI+I  SR+ A SDLRPV  + 
Sbjct: 390 FGNAAAVLQNCNIFARRPRS-KTNTITAQGRSDPNQNTGIIIHNSRVTAASDLRPVLGST 449

Query: 491 PTFLGRPWKEYSRTVIMQSSISDVIDPKGWHEWSGNFALNTLFYGEYQNTGPGASTGGRV 550
            T+LGRPW++YSRTV M++S+  +IDP+GW EW GNFAL TLFY E+QNTGPGAST GRV
Sbjct: 450 KTYLGRPWRQYSRTVFMKTSLDSLIDPRGWLEWDGNFALKTLFYAEFQNTGPGASTSGRV 509

Query: 551 TWKGFRIIRSATEAEGFTAGKFIGGGSWLRATGFPFSLGL 591
           TW GFR++ SA+EA  FT G F+ GGSW+  +  PF+ GL
Sbjct: 510 TWPGFRVLGSASEASKFTVGTFLAGGSWI-PSSVPFTSGL 511