Tan0016743 (gene) Snake gourd v1

Overview
NameTan0016743
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptionDUF4220 domain-containing protein
LocationLG08: 7811899 .. 7814661 (+)
RNA-Seq ExpressionTan0016743
SyntenyTan0016743
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CAAAAGTTATCTCCAAGGTAAGCAATTCTTATTCTTTTAAAAGGAATGAGGAGTGAAGATGCCAATTTCATACAAGATACATGGAAACTAAAATGTAAGAAAATTTTGCTTGCAATTTAAGCATATGTCGGAGAAGATTGCTCCTATCTTGTTATATATCTGATGTATCTTTTCTAAATTCATTAGCTTCTTCGGACAGTTTTATAACTTCTATTCCTATTCATTTTTCTGGTTTTAGAGATGGAGCCTTCTTCTTTTTGAAGTCCCCAAGCTATCTCTTCAGGTATGACCTTCAATTCCAGCTTCTCTCTTCTTTCTTGGAACTCAATATATTTTCCCACCTTTCAAATAGGTGTTTTTTTCCTTTAAGTTTTATTACTGCAAGGATAGAGAATATAAGTTTTGTTCAACTTCTTATAAAGCCTTTTTTTTAGGGTGGGGTGAGTGCTCTGGAAGGAAGGAGATGATTCAGAGATAACAATGGAAATCTATTCTATCTTCTAGCAGTTTTCAAGAATTCTTCACTATTCGACATATCTATTAGAAAGGGAGAACAGGTACCATGTTTTCTGACATATTTGAAAAATTTATACCGAGAAATATCAGTTCCCTATGGAGTTACTGGGGCATTGAGTTGCTAGTATTGGCAAACTTCCTGTTCCAAATTATCCTAACTTTCAATGGAAGTCGTAGAAGGCACACACCGGGATATAAGCTCAGCTTAACTGTTTGGTTTTCCTACTTACTTGCTGCTAAAATTGCAACGGTTGTTCTGGGAAAGCTAACGACAATCGAAATAGGCAAAGACCAGCGTAACACGCACACCCAAATTCAGGCATTGTTGGCACCTTTGATGTTCATGCAAATAGGAAATCCAGATACAATCACAGCCTACTCGATTGAAGACAATCAACTAGGAGTGAGGCAAGTTTTCAGCATGGTGATCCAAGTGGCAATTATGTTTTATATTCTTATAAGGTCATGGACACCGTCCACAACATCATTTCTTTATTTGCCGATGTCTTTGGCTGGGATAATCAAGTATGGTGAGACTTCATGGGCTCTAAAATCAGCACTCAATGGCAACTTCGGCTTCACAATTGCAGATTTCTTCAAATATCATGAAATAGCTCGTTTGTTTGACAAATTGCCCCAGGGAGAGAATGAACTCCCAGAGGCAAGATTGATCTTAAGAGCTTATTATAGGTTTTGCTGCCTAAAACCTCATCTTGAGAATTGGCTTTACTATCCTCCAACTGATTGTGAAAACGAAAAACTATACATCAAGGACTGTGAGTATGAAGATGTGTTCAGAATTACAGATTCTGAACTGGGTTTCATGTACGATGCGCTCTACACTAAGGCACCAGTTGTATATACTCGTAAGGGTTTAATTCTTCGTTTTATTAGCTTGCTTAGCTTGATTGCCACACTAGTTGGGTTTTCTGTCTTGTTCAAGGATGCATTTGTGTATAATATTAGTGCTGGCTTTATCAATTTTGTGTTGATTGCATCTTTGATCATTGAGGTCTATCAGATCTTGAGGCTACCATTCACAGATTGGGCTATAATACAGATGATAAGGCACTATGAAACTTTTCCTTTCCTTCTGGGTTTCTTGAAGTCTCTAGCCCCTCAATCAGCAACTTGGAGAAGGTGGTCTAACACAATGGGGCAGTTCAATCTTTTAGATTTCTGCTTGCAAACCAAGCATCGGAACTACAGCAGAATCAAAATTCTCCGATATTGGGGCATGGATATGAAACTCCGAAAGCAATTGAGTTTGGATCGAGTTGAAGTCCATCCGAAAGTGAAAGAGCTTGTGGTTACAGAACTGAGAGAGATAGATACGATCAAAGGACTAGAAGAATTTGATCAAAGAGGCCAATGGACAATCCAAAGGTATCGAGGAAAACTCAAACTCAGTGATGAAAGCTCATTGATCCAAGCACTTGAAACGACTGTACAAAAGAGACCTTTCGATAAGAGCATCTTCATATGGCACATCACAACAAATATTTTCTATCACATCCGACGTTTTCATGATACTACGGAAGTTACTAAAATGGAAGCTATCATGAATCTATCAGATTATATGATGTACCTTTTAGTGACTCGTTCCCATGTGCTATCATCAACAACTGGAGATATTATATTTGACCATTCATGCGTGAAGCTCGGGAGGTTCACAAGAACTGGACGTCTAAACAAAGAGGATGCCTGCAAAGAACTTTTGGAGTTACAGAAAGAAGGTATGTTTGAGGTAAAGGGGCCAAATGAACCACCTGAATCAGAAGCAGAGAAAGTAGTTGTAGGGAATTGGAATCTGCTGAAAGATGTAAAAGAACTTGCAGATAGCTTATCAACTCTGAGCAAGGAAAACATATGGAAGATAATTGGCAGTATGTGGGTTGAGATGTTGGGATATGCTGCAAGTCACTGTGAAATGGAATATCATTCAGAACACATCAGACAAGGTGGTGAATTGATCACTCATGTTTGGCTTTTGGTAGCCCATAATGTTACCAAATACAGTTCATATGAATATCACCTGAGCAGTCAAGATGAAGAGACTCCAACTACTTCTCGTTAGTTTTTCAACTTCTTTTTCTTGAGAAATGAAATTCGATTGCTTGTAAGGAAGCTTTATGTATTCTCATTTTTTTGGTTCAATAATTTATTTTGTAAAAAGCATAATGCATGGAATTCCCCCAATCTAAATGCTGTATAACAATTTCTAATAAAAAGAAAATTATAATTCAT

mRNA sequence

CAAAAGTTATCTCCAAGGTAAGCAATTCTTATTCTTTTAAAAGGAATGAGGAGTGAAGATGCCAATTTCATACAAGATACATGGAAACTAAAATGTAAGAAAATTTTGCTTGCAATTTAAGCATATGTCGGAGAAGATTGCTCCTATCTTGTTATATATCTGATGTATCTTTTCTAAATTCATTAGCTTCTTCGGACAGTTTTATAACTTCTATTCCTATTCATTTTTCTGGTTTTAGAGATGGAGCCTTCTTCTTTTTGAAGTCCCCAAGCTATCTCTTCAGGGTGGGGTGAGTGCTCTGGAAGGAAGGAGATGATTCAGAGATAACAATGGAAATCTATTCTATCTTCTAGCAGTTTTCAAGAATTCTTCACTATTCGACATATCTATTAGAAAGGGAGAACAGGTACCATGTTTTCTGACATATTTGAAAAATTTATACCGAGAAATATCAGTTCCCTATGGAGTTACTGGGGCATTGAGTTGCTAGTATTGGCAAACTTCCTGTTCCAAATTATCCTAACTTTCAATGGAAGTCGTAGAAGGCACACACCGGGATATAAGCTCAGCTTAACTGTTTGGTTTTCCTACTTACTTGCTGCTAAAATTGCAACGGTTGTTCTGGGAAAGCTAACGACAATCGAAATAGGCAAAGACCAGCGTAACACGCACACCCAAATTCAGGCATTGTTGGCACCTTTGATGTTCATGCAAATAGGAAATCCAGATACAATCACAGCCTACTCGATTGAAGACAATCAACTAGGAGTGAGGCAAGTTTTCAGCATGGTGATCCAAGTGGCAATTATGTTTTATATTCTTATAAGGTCATGGACACCGTCCACAACATCATTTCTTTATTTGCCGATGTCTTTGGCTGGGATAATCAAGTATGGTGAGACTTCATGGGCTCTAAAATCAGCACTCAATGGCAACTTCGGCTTCACAATTGCAGATTTCTTCAAATATCATGAAATAGCTCGTTTGTTTGACAAATTGCCCCAGGGAGAGAATGAACTCCCAGAGGCAAGATTGATCTTAAGAGCTTATTATAGGTTTTGCTGCCTAAAACCTCATCTTGAGAATTGGCTTTACTATCCTCCAACTGATTGTGAAAACGAAAAACTATACATCAAGGACTGTGAGTATGAAGATGTGTTCAGAATTACAGATTCTGAACTGGGTTTCATGTACGATGCGCTCTACACTAAGGCACCAGTTGTATATACTCGTAAGGGTTTAATTCTTCGTTTTATTAGCTTGCTTAGCTTGATTGCCACACTAGTTGGGTTTTCTGTCTTGTTCAAGGATGCATTTGTGTATAATATTAGTGCTGGCTTTATCAATTTTGTGTTGATTGCATCTTTGATCATTGAGGTCTATCAGATCTTGAGGCTACCATTCACAGATTGGGCTATAATACAGATGATAAGGCACTATGAAACTTTTCCTTTCCTTCTGGGTTTCTTGAAGTCTCTAGCCCCTCAATCAGCAACTTGGAGAAGGTGGTCTAACACAATGGGGCAGTTCAATCTTTTAGATTTCTGCTTGCAAACCAAGCATCGGAACTACAGCAGAATCAAAATTCTCCGATATTGGGGCATGGATATGAAACTCCGAAAGCAATTGAGTTTGGATCGAGTTGAAGTCCATCCGAAAGTGAAAGAGCTTGTGGTTACAGAACTGAGAGAGATAGATACGATCAAAGGACTAGAAGAATTTGATCAAAGAGGCCAATGGACAATCCAAAGGTATCGAGGAAAACTCAAACTCAGTGATGAAAGCTCATTGATCCAAGCACTTGAAACGACTGTACAAAAGAGACCTTTCGATAAGAGCATCTTCATATGGCACATCACAACAAATATTTTCTATCACATCCGACGTTTTCATGATACTACGGAAGTTACTAAAATGGAAGCTATCATGAATCTATCAGATTATATGATGTACCTTTTAGTGACTCGTTCCCATGTGCTATCATCAACAACTGGAGATATTATATTTGACCATTCATGCGTGAAGCTCGGGAGGTTCACAAGAACTGGACGTCTAAACAAAGAGGATGCCTGCAAAGAACTTTTGGAGTTACAGAAAGAAGGTATGTTTGAGGTAAAGGGGCCAAATGAACCACCTGAATCAGAAGCAGAGAAAGTAGTTGTAGGGAATTGGAATCTGCTGAAAGATGTAAAAGAACTTGCAGATAGCTTATCAACTCTGAGCAAGGAAAACATATGGAAGATAATTGGCAGTATGTGGGTTGAGATGTTGGGATATGCTGCAAGTCACTGTGAAATGGAATATCATTCAGAACACATCAGACAAGGTGGTGAATTGATCACTCATGTTTGGCTTTTGGTAGCCCATAATGTTACCAAATACAGTTCATATGAATATCACCTGAGCAGTCAAGATGAAGAGACTCCAACTACTTCTCGTTAGTTTTTCAACTTCTTTTTCTTGAGAAATGAAATTCGATTGCTTGTAAGGAAGCTTTATGTATTCTCATTTTTTTGGTTCAATAATTTATTTTGTAAAAAGCATAATGCATGGAATTCCCCCAATCTAAATGCTGTATAACAATTTCTAATAAAAAGAAAATTATAATTCAT

Coding sequence (CDS)

ATGTTTTCTGACATATTTGAAAAATTTATACCGAGAAATATCAGTTCCCTATGGAGTTACTGGGGCATTGAGTTGCTAGTATTGGCAAACTTCCTGTTCCAAATTATCCTAACTTTCAATGGAAGTCGTAGAAGGCACACACCGGGATATAAGCTCAGCTTAACTGTTTGGTTTTCCTACTTACTTGCTGCTAAAATTGCAACGGTTGTTCTGGGAAAGCTAACGACAATCGAAATAGGCAAAGACCAGCGTAACACGCACACCCAAATTCAGGCATTGTTGGCACCTTTGATGTTCATGCAAATAGGAAATCCAGATACAATCACAGCCTACTCGATTGAAGACAATCAACTAGGAGTGAGGCAAGTTTTCAGCATGGTGATCCAAGTGGCAATTATGTTTTATATTCTTATAAGGTCATGGACACCGTCCACAACATCATTTCTTTATTTGCCGATGTCTTTGGCTGGGATAATCAAGTATGGTGAGACTTCATGGGCTCTAAAATCAGCACTCAATGGCAACTTCGGCTTCACAATTGCAGATTTCTTCAAATATCATGAAATAGCTCGTTTGTTTGACAAATTGCCCCAGGGAGAGAATGAACTCCCAGAGGCAAGATTGATCTTAAGAGCTTATTATAGGTTTTGCTGCCTAAAACCTCATCTTGAGAATTGGCTTTACTATCCTCCAACTGATTGTGAAAACGAAAAACTATACATCAAGGACTGTGAGTATGAAGATGTGTTCAGAATTACAGATTCTGAACTGGGTTTCATGTACGATGCGCTCTACACTAAGGCACCAGTTGTATATACTCGTAAGGGTTTAATTCTTCGTTTTATTAGCTTGCTTAGCTTGATTGCCACACTAGTTGGGTTTTCTGTCTTGTTCAAGGATGCATTTGTGTATAATATTAGTGCTGGCTTTATCAATTTTGTGTTGATTGCATCTTTGATCATTGAGGTCTATCAGATCTTGAGGCTACCATTCACAGATTGGGCTATAATACAGATGATAAGGCACTATGAAACTTTTCCTTTCCTTCTGGGTTTCTTGAAGTCTCTAGCCCCTCAATCAGCAACTTGGAGAAGGTGGTCTAACACAATGGGGCAGTTCAATCTTTTAGATTTCTGCTTGCAAACCAAGCATCGGAACTACAGCAGAATCAAAATTCTCCGATATTGGGGCATGGATATGAAACTCCGAAAGCAATTGAGTTTGGATCGAGTTGAAGTCCATCCGAAAGTGAAAGAGCTTGTGGTTACAGAACTGAGAGAGATAGATACGATCAAAGGACTAGAAGAATTTGATCAAAGAGGCCAATGGACAATCCAAAGGTATCGAGGAAAACTCAAACTCAGTGATGAAAGCTCATTGATCCAAGCACTTGAAACGACTGTACAAAAGAGACCTTTCGATAAGAGCATCTTCATATGGCACATCACAACAAATATTTTCTATCACATCCGACGTTTTCATGATACTACGGAAGTTACTAAAATGGAAGCTATCATGAATCTATCAGATTATATGATGTACCTTTTAGTGACTCGTTCCCATGTGCTATCATCAACAACTGGAGATATTATATTTGACCATTCATGCGTGAAGCTCGGGAGGTTCACAAGAACTGGACGTCTAAACAAAGAGGATGCCTGCAAAGAACTTTTGGAGTTACAGAAAGAAGGTATGTTTGAGGTAAAGGGGCCAAATGAACCACCTGAATCAGAAGCAGAGAAAGTAGTTGTAGGGAATTGGAATCTGCTGAAAGATGTAAAAGAACTTGCAGATAGCTTATCAACTCTGAGCAAGGAAAACATATGGAAGATAATTGGCAGTATGTGGGTTGAGATGTTGGGATATGCTGCAAGTCACTGTGAAATGGAATATCATTCAGAACACATCAGACAAGGTGGTGAATTGATCACTCATGTTTGGCTTTTGGTAGCCCATAATGTTACCAAATACAGTTCATATGAATATCACCTGAGCAGTCAAGATGAAGAGACTCCAACTACTTCTCGTTAG

Protein sequence

MFSDIFEKFIPRNISSLWSYWGIELLVLANFLFQIILTFNGSRRRHTPGYKLSLTVWFSYLLAAKIATVVLGKLTTIEIGKDQRNTHTQIQALLAPLMFMQIGNPDTITAYSIEDNQLGVRQVFSMVIQVAIMFYILIRSWTPSTTSFLYLPMSLAGIIKYGETSWALKSALNGNFGFTIADFFKYHEIARLFDKLPQGENELPEARLILRAYYRFCCLKPHLENWLYYPPTDCENEKLYIKDCEYEDVFRITDSELGFMYDALYTKAPVVYTRKGLILRFISLLSLIATLVGFSVLFKDAFVYNISAGFINFVLIASLIIEVYQILRLPFTDWAIIQMIRHYETFPFLLGFLKSLAPQSATWRRWSNTMGQFNLLDFCLQTKHRNYSRIKILRYWGMDMKLRKQLSLDRVEVHPKVKELVVTELREIDTIKGLEEFDQRGQWTIQRYRGKLKLSDESSLIQALETTVQKRPFDKSIFIWHITTNIFYHIRRFHDTTEVTKMEAIMNLSDYMMYLLVTRSHVLSSTTGDIIFDHSCVKLGRFTRTGRLNKEDACKELLELQKEGMFEVKGPNEPPESEAEKVVVGNWNLLKDVKELADSLSTLSKENIWKIIGSMWVEMLGYAASHCEMEYHSEHIRQGGELITHVWLLVAHNVTKYSSYEYHLSSQDEETPTTSR
Homology
BLAST of Tan0016743 vs. NCBI nr
Match: XP_023538013.1 (uncharacterized protein LOC111798897 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1237.6 bits (3201), Expect = 0.0e+00
Identity = 610/671 (90.91%), Postives = 639/671 (95.23%), Query Frame = 0

Query: 1   MFSDIFEKFIPRNISSLWSYWGIELLVLANFLFQIILTFNGSRRRHTPGYKLSLTVWFSY 60
           MFS++FEK IPR+ISSLWSYWGIELLVLANF+FQIILTFNG RRRHTPGYKLSLTVWFSY
Sbjct: 1   MFSELFEKIIPRDISSLWSYWGIELLVLANFMFQIILTFNGCRRRHTPGYKLSLTVWFSY 60

Query: 61  LLAAKIATVVLGKLTTIEIGKDQRNTHTQIQALLAPLMFMQIGNPDTITAYSIEDNQLGV 120
           LLAAK+ATVVLGKLTTIEIGKDQRNTHTQIQALLAPLMFMQIGNPDTITAYSIEDNQLGV
Sbjct: 61  LLAAKLATVVLGKLTTIEIGKDQRNTHTQIQALLAPLMFMQIGNPDTITAYSIEDNQLGV 120

Query: 121 RQVFSMVIQVAIMFYILIRSWTPSTTSFLYLPMSLAGIIKYGETSWALKSALNGNFGFTI 180
           RQ+FS+VIQV IMFYILIRSWT S TSFLYLPMSLAGIIKY ETSWALKSALNGNFGFTI
Sbjct: 121 RQIFSLVIQVTIMFYILIRSWTNSRTSFLYLPMSLAGIIKYAETSWALKSALNGNFGFTI 180

Query: 181 ADFFKYHEIARLFDKLPQGENELPEARLILRAYYRFCCLKPHLENWLYYPPTDCENEKLY 240
           ADFFKYHE+ARLFDKLPQGENELPEA+LILRAYYRFCCLKPHLENWLYYPPTDCE +KLY
Sbjct: 181 ADFFKYHEVARLFDKLPQGENELPEAKLILRAYYRFCCLKPHLENWLYYPPTDCEYQKLY 240

Query: 241 IKDCEYEDVFRITDSELGFMYDALYTKAPVVYTRKGLILRFISLLSLIATLVGFSVLFKD 300
           I DC+YEDVFRITDSELGFMYDALYTKAPVVYTRKGLILR ISLLSLIATLVGFSVLFKD
Sbjct: 241 IDDCDYEDVFRITDSELGFMYDALYTKAPVVYTRKGLILRCISLLSLIATLVGFSVLFKD 300

Query: 301 AFVYNISAGFINFVLIASLIIEVYQILRLPFTDWAIIQMIRHYETFPFLLGFLKSLAPQS 360
           AFVYNIS GFI+FVLIASLIIEVYQILRLP+TDWAIIQMIRHYETFPFL+GFL+SLAPQS
Sbjct: 301 AFVYNISVGFIHFVLIASLIIEVYQILRLPYTDWAIIQMIRHYETFPFLMGFLQSLAPQS 360

Query: 361 ATWRRWSNTMGQFNLLDFCLQTKHRNYSRIKILRYWGMDMKLRKQLSLDRVEVHPKVKEL 420
           ATWRRWSNTMGQFNLLDFCLQTKHRNYSRIKILRYWGMDMKLRKQLSLDR++V PKVKEL
Sbjct: 361 ATWRRWSNTMGQFNLLDFCLQTKHRNYSRIKILRYWGMDMKLRKQLSLDRIDVDPKVKEL 420

Query: 421 VVTELREIDTIKGLEEFDQRGQWTIQRYRGKLKLSDESSLIQALETTVQKRPFDKSIFIW 480
           VV ELREID IKG EEFDQRGQWTI RYR KLKL+D   +IQALETTV KRPFDKSIFIW
Sbjct: 421 VVAELREIDKIKGQEEFDQRGQWTIGRYREKLKLND---VIQALETTVDKRPFDKSIFIW 480

Query: 481 HITTNIFYHIRRFHDTTEVTKMEAIMNLSDYMMYLLVTRSHVLSSTTGDIIFDHSCVKLG 540
           HITTNIFYHI  FHDTT++ KMEAIMN+SDYMMYLLVTRSHVLSSTTGDIIFDHSCVKLG
Sbjct: 481 HITTNIFYHIESFHDTTDIAKMEAIMNISDYMMYLLVTRSHVLSSTTGDIIFDHSCVKLG 540

Query: 541 RFTRTGRLNKEDACKELLELQKEGMFEVKGPNEPPESEAEKVVVGNWNLLKDVKELADSL 600
           R TRTGRLNKE+ACK+LL LQKEG+ +VK P+ PPESEAEKVVVGNWNLLKDVKELADSL
Sbjct: 541 RLTRTGRLNKEEACKDLLGLQKEGILQVKEPHAPPESEAEKVVVGNWNLLKDVKELADSL 600

Query: 601 STLSKENIWKIIGSMWVEMLGYAASHCEMEYHSEHIRQGGELITHVWLLVAHNVTKYSSY 660
           ST+S ENIWK+IGSMWVEMLGYAASHCEMEYHSEHIR GGELITHVWLLVAHNVTKYSSY
Sbjct: 601 STMSNENIWKVIGSMWVEMLGYAASHCEMEYHSEHIRHGGELITHVWLLVAHNVTKYSSY 660

Query: 661 EYHLSSQDEET 672
           EYH  SQDEET
Sbjct: 661 EYHFGSQDEET 668

BLAST of Tan0016743 vs. NCBI nr
Match: XP_022969679.1 (uncharacterized protein LOC111468632 [Cucurbita maxima])

HSP 1 Score: 1237.2 bits (3200), Expect = 0.0e+00
Identity = 610/671 (90.91%), Postives = 641/671 (95.53%), Query Frame = 0

Query: 1   MFSDIFEKFIPRNISSLWSYWGIELLVLANFLFQIILTFNGSRRRHTPGYKLSLTVWFSY 60
           MFS++FEK IPR+ISSLWSYWGIELLVLANF+FQIILTFNG RRRHTPGYKLSLTVWFSY
Sbjct: 1   MFSELFEKIIPRDISSLWSYWGIELLVLANFMFQIILTFNGCRRRHTPGYKLSLTVWFSY 60

Query: 61  LLAAKIATVVLGKLTTIEIGKDQRNTHTQIQALLAPLMFMQIGNPDTITAYSIEDNQLGV 120
           LLAAK+ATVVLGKLTTIEIGKDQRNTHTQIQALLAPLMFMQIGNPDTITAYSIEDNQLGV
Sbjct: 61  LLAAKLATVVLGKLTTIEIGKDQRNTHTQIQALLAPLMFMQIGNPDTITAYSIEDNQLGV 120

Query: 121 RQVFSMVIQVAIMFYILIRSWTPSTTSFLYLPMSLAGIIKYGETSWALKSALNGNFGFTI 180
           RQ+FS+VIQV+IMFYILIRSWT S TSFLYLPMSLAGIIKY ETSWALKSALNGNFGFTI
Sbjct: 121 RQIFSLVIQVSIMFYILIRSWTNSRTSFLYLPMSLAGIIKYAETSWALKSALNGNFGFTI 180

Query: 181 ADFFKYHEIARLFDKLPQGENELPEARLILRAYYRFCCLKPHLENWLYYPPTDCENEKLY 240
           ADFFKYHE+ARLFDKLPQGENELPEA+LILRAYYRFCCLKPHLENWLYYPPTDCE +KLY
Sbjct: 181 ADFFKYHEVARLFDKLPQGENELPEAKLILRAYYRFCCLKPHLENWLYYPPTDCEYQKLY 240

Query: 241 IKDCEYEDVFRITDSELGFMYDALYTKAPVVYTRKGLILRFISLLSLIATLVGFSVLFKD 300
           I DC+YEDVFRITDSELGFMYDALYTKAPVVYTRKGLILR ISLLSLIATLVGFSVLFKD
Sbjct: 241 IDDCDYEDVFRITDSELGFMYDALYTKAPVVYTRKGLILRCISLLSLIATLVGFSVLFKD 300

Query: 301 AFVYNISAGFINFVLIASLIIEVYQILRLPFTDWAIIQMIRHYETFPFLLGFLKSLAPQS 360
           AFVYNIS GFI+FVLIASLIIEVYQILRLP+TDWAIIQMIRHYETFPFL+GFL+SLAPQS
Sbjct: 301 AFVYNISVGFIHFVLIASLIIEVYQILRLPYTDWAIIQMIRHYETFPFLMGFLQSLAPQS 360

Query: 361 ATWRRWSNTMGQFNLLDFCLQTKHRNYSRIKILRYWGMDMKLRKQLSLDRVEVHPKVKEL 420
           ATWRRWSNTMGQFNLLDFCLQTKHRNYSRIKILRYWGMDMKLRKQLSLDR++V PKVKEL
Sbjct: 361 ATWRRWSNTMGQFNLLDFCLQTKHRNYSRIKILRYWGMDMKLRKQLSLDRIDVDPKVKEL 420

Query: 421 VVTELREIDTIKGLEEFDQRGQWTIQRYRGKLKLSDESSLIQALETTVQKRPFDKSIFIW 480
           VV ELREID IKG EEFDQRGQWTI RYR KLKL+D   +IQALETTV KRPFDKSIFIW
Sbjct: 421 VVAELREIDKIKGQEEFDQRGQWTIGRYREKLKLND---VIQALETTVAKRPFDKSIFIW 480

Query: 481 HITTNIFYHIRRFHDTTEVTKMEAIMNLSDYMMYLLVTRSHVLSSTTGDIIFDHSCVKLG 540
           HITTNIFYHI+ FHDTT+ TKMEAIMN+SDYMMYLLVTRSHVLSSTTGDIIFDHSCVKLG
Sbjct: 481 HITTNIFYHIQSFHDTTDTTKMEAIMNISDYMMYLLVTRSHVLSSTTGDIIFDHSCVKLG 540

Query: 541 RFTRTGRLNKEDACKELLELQKEGMFEVKGPNEPPESEAEKVVVGNWNLLKDVKELADSL 600
           R TRTGRLNKE+AC++LL LQKEG+ +VK P+ PPESEAEKVVVGNWNLLKDVKELADSL
Sbjct: 541 RLTRTGRLNKEEACQDLLGLQKEGILQVKEPHAPPESEAEKVVVGNWNLLKDVKELADSL 600

Query: 601 STLSKENIWKIIGSMWVEMLGYAASHCEMEYHSEHIRQGGELITHVWLLVAHNVTKYSSY 660
           ST+S ENIWK+IGSMWVEMLGYAASHCEMEYHSEHIR GGELITHVWLLVAHNVTKYSSY
Sbjct: 601 STMSNENIWKVIGSMWVEMLGYAASHCEMEYHSEHIRHGGELITHVWLLVAHNVTKYSSY 660

Query: 661 EYHLSSQDEET 672
           EYH  SQDEET
Sbjct: 661 EYHFGSQDEET 668

BLAST of Tan0016743 vs. NCBI nr
Match: XP_022937890.1 (uncharacterized protein LOC111444144 [Cucurbita moschata] >KAG6586039.1 hypothetical protein SDJN03_18772, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1234.9 bits (3194), Expect = 0.0e+00
Identity = 609/671 (90.76%), Postives = 639/671 (95.23%), Query Frame = 0

Query: 1   MFSDIFEKFIPRNISSLWSYWGIELLVLANFLFQIILTFNGSRRRHTPGYKLSLTVWFSY 60
           MFS++FEK IPR+ISSLWSYWGIELLVLANF+FQIILTFNG RRRHTPGYKLSLTVWFSY
Sbjct: 1   MFSELFEKIIPRDISSLWSYWGIELLVLANFMFQIILTFNGCRRRHTPGYKLSLTVWFSY 60

Query: 61  LLAAKIATVVLGKLTTIEIGKDQRNTHTQIQALLAPLMFMQIGNPDTITAYSIEDNQLGV 120
           LLAAK+ATVVLGKLTTIEIGKDQRNTHTQIQALLAPLMFMQIGNPDTITAYSIEDNQLGV
Sbjct: 61  LLAAKLATVVLGKLTTIEIGKDQRNTHTQIQALLAPLMFMQIGNPDTITAYSIEDNQLGV 120

Query: 121 RQVFSMVIQVAIMFYILIRSWTPSTTSFLYLPMSLAGIIKYGETSWALKSALNGNFGFTI 180
           RQ+FS+VIQV IMFYILIRSWT S TSFLYLPMSLAGIIKY ETSWALKSALNGNFGFTI
Sbjct: 121 RQIFSLVIQVTIMFYILIRSWTNSRTSFLYLPMSLAGIIKYAETSWALKSALNGNFGFTI 180

Query: 181 ADFFKYHEIARLFDKLPQGENELPEARLILRAYYRFCCLKPHLENWLYYPPTDCENEKLY 240
           ADFFKYHE+ARLFDKLPQGENELPEA+LILRAYYRFCCLKPHLENWLYYPPTDCE +KLY
Sbjct: 181 ADFFKYHEVARLFDKLPQGENELPEAKLILRAYYRFCCLKPHLENWLYYPPTDCEYQKLY 240

Query: 241 IKDCEYEDVFRITDSELGFMYDALYTKAPVVYTRKGLILRFISLLSLIATLVGFSVLFKD 300
           I DC+YEDVFRITDSELGFMYDALYTKAPVVYTRKGLILR ISLLSLIATLVGFSVLFKD
Sbjct: 241 IDDCDYEDVFRITDSELGFMYDALYTKAPVVYTRKGLILRCISLLSLIATLVGFSVLFKD 300

Query: 301 AFVYNISAGFINFVLIASLIIEVYQILRLPFTDWAIIQMIRHYETFPFLLGFLKSLAPQS 360
           AFVYNIS GFI+FVLIASLIIEVYQILRLP+TDWAIIQMIRHYETFPFL+GFL+SLAPQS
Sbjct: 301 AFVYNISVGFIHFVLIASLIIEVYQILRLPYTDWAIIQMIRHYETFPFLMGFLQSLAPQS 360

Query: 361 ATWRRWSNTMGQFNLLDFCLQTKHRNYSRIKILRYWGMDMKLRKQLSLDRVEVHPKVKEL 420
           ATWRRWSNTMGQFNLLDFCLQTKHRNYSRIKILRYWGMDMKLRKQLSLDR++V PKVKEL
Sbjct: 361 ATWRRWSNTMGQFNLLDFCLQTKHRNYSRIKILRYWGMDMKLRKQLSLDRIDVDPKVKEL 420

Query: 421 VVTELREIDTIKGLEEFDQRGQWTIQRYRGKLKLSDESSLIQALETTVQKRPFDKSIFIW 480
           VV ELREID IKG EEFDQRGQWTI RYR KLKL+D   +IQALETTV KRPFDKSIFIW
Sbjct: 421 VVAELREIDKIKGQEEFDQRGQWTIGRYREKLKLND---VIQALETTVNKRPFDKSIFIW 480

Query: 481 HITTNIFYHIRRFHDTTEVTKMEAIMNLSDYMMYLLVTRSHVLSSTTGDIIFDHSCVKLG 540
           HITTNIFYHI+ FHDTT+  KMEAIMN+SDYMMYLLVTRSHVLSSTTGDIIFDHSCVKLG
Sbjct: 481 HITTNIFYHIQSFHDTTDNAKMEAIMNISDYMMYLLVTRSHVLSSTTGDIIFDHSCVKLG 540

Query: 541 RFTRTGRLNKEDACKELLELQKEGMFEVKGPNEPPESEAEKVVVGNWNLLKDVKELADSL 600
           R TRTGRLNKE+ACK+LL LQKEG+ +VK P+ PPESEAEKVVVGNWNLLKDVKELADSL
Sbjct: 541 RLTRTGRLNKEEACKDLLGLQKEGILQVKEPHAPPESEAEKVVVGNWNLLKDVKELADSL 600

Query: 601 STLSKENIWKIIGSMWVEMLGYAASHCEMEYHSEHIRQGGELITHVWLLVAHNVTKYSSY 660
           ST+S ENIWK++GSMWVEMLGYAASHCEMEYHSEHIR GGELITHVWLLVAHNVTKYSSY
Sbjct: 601 STMSNENIWKVLGSMWVEMLGYAASHCEMEYHSEHIRHGGELITHVWLLVAHNVTKYSSY 660

Query: 661 EYHLSSQDEET 672
           EYH  SQDEET
Sbjct: 661 EYHFGSQDEET 668

BLAST of Tan0016743 vs. NCBI nr
Match: KAG7020815.1 (hypothetical protein SDJN02_17503, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1177.9 bits (3046), Expect = 0.0e+00
Identity = 582/640 (90.94%), Postives = 609/640 (95.16%), Query Frame = 0

Query: 32  LFQIILTFNGSRRRHTPGYKLSLTVWFSYLLAAKIATVVLGKLTTIEIGKDQRNTHTQIQ 91
           +FQIILTFNG RRRHTPGYKLSLTVWFSYLLAAK+ATVVLGKLTTIEIGKDQRNTHTQIQ
Sbjct: 1   MFQIILTFNGCRRRHTPGYKLSLTVWFSYLLAAKLATVVLGKLTTIEIGKDQRNTHTQIQ 60

Query: 92  ALLAPLMFMQIGNPDTITAYSIEDNQLGVRQVFSMVIQVAIMFYILIRSWTPSTTSFLYL 151
           ALLAPLMFMQIGNPDTITAYSIEDNQLGVRQ+FS+VIQV IMFYILIRSWT S TSFLYL
Sbjct: 61  ALLAPLMFMQIGNPDTITAYSIEDNQLGVRQIFSLVIQVTIMFYILIRSWTNSRTSFLYL 120

Query: 152 PMSLAGIIKYGETSWALKSALNGNFGFTIADFFKYHEIARLFDKLPQGENELPEARLILR 211
           PMSLAGIIKY ETSWALKSALNGNFGFTIADFFKYHE+ARLFDKLPQGENELPEA+LILR
Sbjct: 121 PMSLAGIIKYAETSWALKSALNGNFGFTIADFFKYHEVARLFDKLPQGENELPEAKLILR 180

Query: 212 AYYRFCCLKPHLENWLYYPPTDCENEKLYIKDCEYEDVFRITDSELGFMYDALYTKAPVV 271
           AYYRFCCLKPHLENWLYYPPTDCE +KLYI DC+YEDVFRITDSELGFMYDALYTKAPVV
Sbjct: 181 AYYRFCCLKPHLENWLYYPPTDCEYQKLYIDDCDYEDVFRITDSELGFMYDALYTKAPVV 240

Query: 272 YTRKGLILRFISLLSLIATLVGFSVLFKDAFVYNISAGFINFVLIASLIIEVYQILRLPF 331
           YTRKGLILR ISLLSLIATLVGFSVLFKDAFVYNIS GFI+FVLIASLIIEVYQILRLP+
Sbjct: 241 YTRKGLILRCISLLSLIATLVGFSVLFKDAFVYNISVGFIHFVLIASLIIEVYQILRLPY 300

Query: 332 TDWAIIQMIRHYETFPFLLGFLKSLAPQSATWRRWSNTMGQFNLLDFCLQTKHRNYSRIK 391
           TDWAIIQMIRHYETFPFL+GFL+SLAPQSATWRRWSNTMGQFNLLDFCLQTKHRNYSRIK
Sbjct: 301 TDWAIIQMIRHYETFPFLMGFLQSLAPQSATWRRWSNTMGQFNLLDFCLQTKHRNYSRIK 360

Query: 392 ILRYWGMDMKLRKQLSLDRVEVHPKVKELVVTELREIDTIKGLEEFDQRGQWTIQRYRGK 451
           ILRYWGMDMKLRKQLSLDR++V PKVKELVV ELREID IKG EEFDQRGQWTI RYR K
Sbjct: 361 ILRYWGMDMKLRKQLSLDRIDVDPKVKELVVAELREIDKIKGQEEFDQRGQWTIGRYREK 420

Query: 452 LKLSDESSLIQALETTVQKRPFDKSIFIWHITTNIFYHIRRFHDTTEVTKMEAIMNLSDY 511
           LKL+D   +IQALETTV KRPFDKSIFIWHITTNIFYHI+ FHDTT+  KMEAIMN+SDY
Sbjct: 421 LKLND---VIQALETTVNKRPFDKSIFIWHITTNIFYHIQSFHDTTDNAKMEAIMNISDY 480

Query: 512 MMYLLVTRSHVLSSTTGDIIFDHSCVKLGRFTRTGRLNKEDACKELLELQKEGMFEVKGP 571
           MMYLLVTRSHVLSSTTGDIIFDHSCVKLGR TRTGRLNKE+ACK+LL LQKEG+ +VK P
Sbjct: 481 MMYLLVTRSHVLSSTTGDIIFDHSCVKLGRLTRTGRLNKEEACKDLLGLQKEGILQVKEP 540

Query: 572 NEPPESEAEKVVVGNWNLLKDVKELADSLSTLSKENIWKIIGSMWVEMLGYAASHCEMEY 631
           + PPESEAEKVVVGNWNLLKDVKELADSLST+S ENIWK++GSMWVEMLGYAASHCEMEY
Sbjct: 541 HAPPESEAEKVVVGNWNLLKDVKELADSLSTMSNENIWKVLGSMWVEMLGYAASHCEMEY 600

Query: 632 HSEHIRQGGELITHVWLLVAHNVTKYSSYEYHLSSQDEET 672
           HSEHIR GGELITHVWLLVAHNVTKYSSYEYH  SQDEET
Sbjct: 601 HSEHIRHGGELITHVWLLVAHNVTKYSSYEYHFGSQDEET 637

BLAST of Tan0016743 vs. NCBI nr
Match: XP_038889477.1 (uncharacterized protein LOC120079385 [Benincasa hispida])

HSP 1 Score: 1161.4 bits (3003), Expect = 0.0e+00
Identity = 565/675 (83.70%), Postives = 620/675 (91.85%), Query Frame = 0

Query: 1   MFSDIFEKFIPRNISSLWSYWGIELLVLANFLFQIILTFNGSRRRHTPGYKLSLTVWFSY 60
           MFS++FE  +P +IS +WSYWGIELLVLANF+FQ+ILTFNGSRRRHTPG KLSLTVWFSY
Sbjct: 1   MFSELFELIVPGHISFIWSYWGIELLVLANFVFQVILTFNGSRRRHTPGNKLSLTVWFSY 60

Query: 61  LLAAKIATVVLGKLTTIEIGKDQRNTHTQIQALLAPLMFMQIGNPDTITAYSIEDNQLGV 120
           LLAAKIATVVLGKLTTIEIG+++RNTHTQ+QALLAPLMFMQIGNPDTITAYSIEDNQLGV
Sbjct: 61  LLAAKIATVVLGKLTTIEIGREKRNTHTQVQALLAPLMFMQIGNPDTITAYSIEDNQLGV 120

Query: 121 RQVFSMVIQVAIMFYILIRSWTPSTTSFLYLPMSLAGIIKYGETSWALKSALNGNFGFTI 180
           RQVFSMVIQVAIMFYILIRSWT S TSFLY+PMS+AGIIKYGETSWALKSALNGNFGFTI
Sbjct: 121 RQVFSMVIQVAIMFYILIRSWTNSKTSFLYIPMSVAGIIKYGETSWALKSALNGNFGFTI 180

Query: 181 ADFFKYHEIARLFDKLPQGENELPEARLILRAYYRFCCLKPHLENWLYYPPTDCENEKLY 240
           ADFFKYHE+A LF KLPQGENELPEA LILRAYYRFCCLKPHLENWLYYPPTDC+ EKLY
Sbjct: 181 ADFFKYHEVADLFKKLPQGENELPEANLILRAYYRFCCLKPHLENWLYYPPTDCDQEKLY 240

Query: 241 IKDCEYEDVFRITDSELGFMYDALYTKAPVVYTRKGLILRFISLLSLIATLVGFSVLFKD 300
           I DC+YEDVFRITDSELGFMYDALYTKAPVVYTRKGLILRFISLLS+IATL GFSVLFKD
Sbjct: 241 IDDCKYEDVFRITDSELGFMYDALYTKAPVVYTRKGLILRFISLLSVIATLCGFSVLFKD 300

Query: 301 AFVYNISAGFINFVLIASLIIEVYQILRLPFTDWAIIQMIRHYETFPFLLGFLKSLAPQS 360
           AFVYNIS GFI+FVLIA+LIIE+YQILRLPFTDWAI+QM+RH+E FP L GFL+SLAPQS
Sbjct: 301 AFVYNISIGFIHFVLIAALIIEIYQILRLPFTDWAIVQMVRHHEAFPILGGFLRSLAPQS 360

Query: 361 ATWRRWSNTMGQFNLLDFCLQTKHRNYSRIKILRYWGMDMKLRKQLSLDRVEVHPKVKEL 420
           ATWRRWSNT+GQFNLLDFCLQTKHRNYSRIKILRYWGMDMKLRKQLSLDR+++ P+VKEL
Sbjct: 361 ATWRRWSNTLGQFNLLDFCLQTKHRNYSRIKILRYWGMDMKLRKQLSLDRIDIPPEVKEL 420

Query: 421 VVTELREIDTIKGLEEFDQRGQWTIQRYRGKLKLSDESSLIQALETTVQKRPFDKSIFIW 480
           VVTELREI+ IKG EEFDQRGQWTI RY+ KL  ++E+ LI+A+ETTV KRPFDK IFIW
Sbjct: 421 VVTELREIEKIKGQEEFDQRGQWTIDRYKKKLNFNNENKLIKAMETTVSKRPFDKCIFIW 480

Query: 481 HITTNIFYHIRRFHDTTEVTKMEAIMNLSDYMMYLLVTRSHVLSSTTGDIIFDHSCVKLG 540
           HITTNIFY+I  + D +   K EAIM+LSDYMMYLLVTRSHVLS+TT DIIFDHSCVKLG
Sbjct: 481 HITTNIFYNIECYRDASVGNKTEAIMSLSDYMMYLLVTRSHVLSTTTADIIFDHSCVKLG 540

Query: 541 RFTRTGRLNKEDACKELLELQKEGMFEVKGPNEPPESEAEKVVVGNWNLLKDVKELADSL 600
           +FTRTGRL KED C ++L LQKE +   + P+EP ESEAEKVVVGNW+L+KDVKELADSL
Sbjct: 541 KFTRTGRLKKEDVCNDILNLQKESILHAREPHEPIESEAEKVVVGNWHLMKDVKELADSL 600

Query: 601 STLSKENIWKIIGSMWVEMLGYAASHCEMEYHSEHIRQGGELITHVWLLVAHNVTKYSSY 660
            TLS E+ WK++GSMW EM+GYAAS CEMEYHSEHIRQGGELITHVWLL+AHNVTKYSSY
Sbjct: 601 LTLSNEDKWKLLGSMWFEMMGYAASKCEMEYHSEHIRQGGELITHVWLLIAHNVTKYSSY 660

Query: 661 EYHLSSQDEETPTTS 676
           EYH+  QDEETP  S
Sbjct: 661 EYHVGGQDEETPPAS 675

BLAST of Tan0016743 vs. ExPASy TrEMBL
Match: A0A6J1I3C0 (uncharacterized protein LOC111468632 OS=Cucurbita maxima OX=3661 GN=LOC111468632 PE=4 SV=1)

HSP 1 Score: 1237.2 bits (3200), Expect = 0.0e+00
Identity = 610/671 (90.91%), Postives = 641/671 (95.53%), Query Frame = 0

Query: 1   MFSDIFEKFIPRNISSLWSYWGIELLVLANFLFQIILTFNGSRRRHTPGYKLSLTVWFSY 60
           MFS++FEK IPR+ISSLWSYWGIELLVLANF+FQIILTFNG RRRHTPGYKLSLTVWFSY
Sbjct: 1   MFSELFEKIIPRDISSLWSYWGIELLVLANFMFQIILTFNGCRRRHTPGYKLSLTVWFSY 60

Query: 61  LLAAKIATVVLGKLTTIEIGKDQRNTHTQIQALLAPLMFMQIGNPDTITAYSIEDNQLGV 120
           LLAAK+ATVVLGKLTTIEIGKDQRNTHTQIQALLAPLMFMQIGNPDTITAYSIEDNQLGV
Sbjct: 61  LLAAKLATVVLGKLTTIEIGKDQRNTHTQIQALLAPLMFMQIGNPDTITAYSIEDNQLGV 120

Query: 121 RQVFSMVIQVAIMFYILIRSWTPSTTSFLYLPMSLAGIIKYGETSWALKSALNGNFGFTI 180
           RQ+FS+VIQV+IMFYILIRSWT S TSFLYLPMSLAGIIKY ETSWALKSALNGNFGFTI
Sbjct: 121 RQIFSLVIQVSIMFYILIRSWTNSRTSFLYLPMSLAGIIKYAETSWALKSALNGNFGFTI 180

Query: 181 ADFFKYHEIARLFDKLPQGENELPEARLILRAYYRFCCLKPHLENWLYYPPTDCENEKLY 240
           ADFFKYHE+ARLFDKLPQGENELPEA+LILRAYYRFCCLKPHLENWLYYPPTDCE +KLY
Sbjct: 181 ADFFKYHEVARLFDKLPQGENELPEAKLILRAYYRFCCLKPHLENWLYYPPTDCEYQKLY 240

Query: 241 IKDCEYEDVFRITDSELGFMYDALYTKAPVVYTRKGLILRFISLLSLIATLVGFSVLFKD 300
           I DC+YEDVFRITDSELGFMYDALYTKAPVVYTRKGLILR ISLLSLIATLVGFSVLFKD
Sbjct: 241 IDDCDYEDVFRITDSELGFMYDALYTKAPVVYTRKGLILRCISLLSLIATLVGFSVLFKD 300

Query: 301 AFVYNISAGFINFVLIASLIIEVYQILRLPFTDWAIIQMIRHYETFPFLLGFLKSLAPQS 360
           AFVYNIS GFI+FVLIASLIIEVYQILRLP+TDWAIIQMIRHYETFPFL+GFL+SLAPQS
Sbjct: 301 AFVYNISVGFIHFVLIASLIIEVYQILRLPYTDWAIIQMIRHYETFPFLMGFLQSLAPQS 360

Query: 361 ATWRRWSNTMGQFNLLDFCLQTKHRNYSRIKILRYWGMDMKLRKQLSLDRVEVHPKVKEL 420
           ATWRRWSNTMGQFNLLDFCLQTKHRNYSRIKILRYWGMDMKLRKQLSLDR++V PKVKEL
Sbjct: 361 ATWRRWSNTMGQFNLLDFCLQTKHRNYSRIKILRYWGMDMKLRKQLSLDRIDVDPKVKEL 420

Query: 421 VVTELREIDTIKGLEEFDQRGQWTIQRYRGKLKLSDESSLIQALETTVQKRPFDKSIFIW 480
           VV ELREID IKG EEFDQRGQWTI RYR KLKL+D   +IQALETTV KRPFDKSIFIW
Sbjct: 421 VVAELREIDKIKGQEEFDQRGQWTIGRYREKLKLND---VIQALETTVAKRPFDKSIFIW 480

Query: 481 HITTNIFYHIRRFHDTTEVTKMEAIMNLSDYMMYLLVTRSHVLSSTTGDIIFDHSCVKLG 540
           HITTNIFYHI+ FHDTT+ TKMEAIMN+SDYMMYLLVTRSHVLSSTTGDIIFDHSCVKLG
Sbjct: 481 HITTNIFYHIQSFHDTTDTTKMEAIMNISDYMMYLLVTRSHVLSSTTGDIIFDHSCVKLG 540

Query: 541 RFTRTGRLNKEDACKELLELQKEGMFEVKGPNEPPESEAEKVVVGNWNLLKDVKELADSL 600
           R TRTGRLNKE+AC++LL LQKEG+ +VK P+ PPESEAEKVVVGNWNLLKDVKELADSL
Sbjct: 541 RLTRTGRLNKEEACQDLLGLQKEGILQVKEPHAPPESEAEKVVVGNWNLLKDVKELADSL 600

Query: 601 STLSKENIWKIIGSMWVEMLGYAASHCEMEYHSEHIRQGGELITHVWLLVAHNVTKYSSY 660
           ST+S ENIWK+IGSMWVEMLGYAASHCEMEYHSEHIR GGELITHVWLLVAHNVTKYSSY
Sbjct: 601 STMSNENIWKVIGSMWVEMLGYAASHCEMEYHSEHIRHGGELITHVWLLVAHNVTKYSSY 660

Query: 661 EYHLSSQDEET 672
           EYH  SQDEET
Sbjct: 661 EYHFGSQDEET 668

BLAST of Tan0016743 vs. ExPASy TrEMBL
Match: A0A6J1FI30 (uncharacterized protein LOC111444144 OS=Cucurbita moschata OX=3662 GN=LOC111444144 PE=4 SV=1)

HSP 1 Score: 1234.9 bits (3194), Expect = 0.0e+00
Identity = 609/671 (90.76%), Postives = 639/671 (95.23%), Query Frame = 0

Query: 1   MFSDIFEKFIPRNISSLWSYWGIELLVLANFLFQIILTFNGSRRRHTPGYKLSLTVWFSY 60
           MFS++FEK IPR+ISSLWSYWGIELLVLANF+FQIILTFNG RRRHTPGYKLSLTVWFSY
Sbjct: 1   MFSELFEKIIPRDISSLWSYWGIELLVLANFMFQIILTFNGCRRRHTPGYKLSLTVWFSY 60

Query: 61  LLAAKIATVVLGKLTTIEIGKDQRNTHTQIQALLAPLMFMQIGNPDTITAYSIEDNQLGV 120
           LLAAK+ATVVLGKLTTIEIGKDQRNTHTQIQALLAPLMFMQIGNPDTITAYSIEDNQLGV
Sbjct: 61  LLAAKLATVVLGKLTTIEIGKDQRNTHTQIQALLAPLMFMQIGNPDTITAYSIEDNQLGV 120

Query: 121 RQVFSMVIQVAIMFYILIRSWTPSTTSFLYLPMSLAGIIKYGETSWALKSALNGNFGFTI 180
           RQ+FS+VIQV IMFYILIRSWT S TSFLYLPMSLAGIIKY ETSWALKSALNGNFGFTI
Sbjct: 121 RQIFSLVIQVTIMFYILIRSWTNSRTSFLYLPMSLAGIIKYAETSWALKSALNGNFGFTI 180

Query: 181 ADFFKYHEIARLFDKLPQGENELPEARLILRAYYRFCCLKPHLENWLYYPPTDCENEKLY 240
           ADFFKYHE+ARLFDKLPQGENELPEA+LILRAYYRFCCLKPHLENWLYYPPTDCE +KLY
Sbjct: 181 ADFFKYHEVARLFDKLPQGENELPEAKLILRAYYRFCCLKPHLENWLYYPPTDCEYQKLY 240

Query: 241 IKDCEYEDVFRITDSELGFMYDALYTKAPVVYTRKGLILRFISLLSLIATLVGFSVLFKD 300
           I DC+YEDVFRITDSELGFMYDALYTKAPVVYTRKGLILR ISLLSLIATLVGFSVLFKD
Sbjct: 241 IDDCDYEDVFRITDSELGFMYDALYTKAPVVYTRKGLILRCISLLSLIATLVGFSVLFKD 300

Query: 301 AFVYNISAGFINFVLIASLIIEVYQILRLPFTDWAIIQMIRHYETFPFLLGFLKSLAPQS 360
           AFVYNIS GFI+FVLIASLIIEVYQILRLP+TDWAIIQMIRHYETFPFL+GFL+SLAPQS
Sbjct: 301 AFVYNISVGFIHFVLIASLIIEVYQILRLPYTDWAIIQMIRHYETFPFLMGFLQSLAPQS 360

Query: 361 ATWRRWSNTMGQFNLLDFCLQTKHRNYSRIKILRYWGMDMKLRKQLSLDRVEVHPKVKEL 420
           ATWRRWSNTMGQFNLLDFCLQTKHRNYSRIKILRYWGMDMKLRKQLSLDR++V PKVKEL
Sbjct: 361 ATWRRWSNTMGQFNLLDFCLQTKHRNYSRIKILRYWGMDMKLRKQLSLDRIDVDPKVKEL 420

Query: 421 VVTELREIDTIKGLEEFDQRGQWTIQRYRGKLKLSDESSLIQALETTVQKRPFDKSIFIW 480
           VV ELREID IKG EEFDQRGQWTI RYR KLKL+D   +IQALETTV KRPFDKSIFIW
Sbjct: 421 VVAELREIDKIKGQEEFDQRGQWTIGRYREKLKLND---VIQALETTVNKRPFDKSIFIW 480

Query: 481 HITTNIFYHIRRFHDTTEVTKMEAIMNLSDYMMYLLVTRSHVLSSTTGDIIFDHSCVKLG 540
           HITTNIFYHI+ FHDTT+  KMEAIMN+SDYMMYLLVTRSHVLSSTTGDIIFDHSCVKLG
Sbjct: 481 HITTNIFYHIQSFHDTTDNAKMEAIMNISDYMMYLLVTRSHVLSSTTGDIIFDHSCVKLG 540

Query: 541 RFTRTGRLNKEDACKELLELQKEGMFEVKGPNEPPESEAEKVVVGNWNLLKDVKELADSL 600
           R TRTGRLNKE+ACK+LL LQKEG+ +VK P+ PPESEAEKVVVGNWNLLKDVKELADSL
Sbjct: 541 RLTRTGRLNKEEACKDLLGLQKEGILQVKEPHAPPESEAEKVVVGNWNLLKDVKELADSL 600

Query: 601 STLSKENIWKIIGSMWVEMLGYAASHCEMEYHSEHIRQGGELITHVWLLVAHNVTKYSSY 660
           ST+S ENIWK++GSMWVEMLGYAASHCEMEYHSEHIR GGELITHVWLLVAHNVTKYSSY
Sbjct: 601 STMSNENIWKVLGSMWVEMLGYAASHCEMEYHSEHIRHGGELITHVWLLVAHNVTKYSSY 660

Query: 661 EYHLSSQDEET 672
           EYH  SQDEET
Sbjct: 661 EYHFGSQDEET 668

BLAST of Tan0016743 vs. ExPASy TrEMBL
Match: A0A6J1KHN9 (uncharacterized protein LOC111493941 OS=Cucurbita maxima OX=3661 GN=LOC111493941 PE=4 SV=1)

HSP 1 Score: 1156.0 bits (2989), Expect = 0.0e+00
Identity = 566/676 (83.73%), Postives = 622/676 (92.01%), Query Frame = 0

Query: 1   MFSDIFEKFIPRNISSLWSYWGIELLVLANFLFQIILTFNGSRRRHTPGYKLSLTVWFSY 60
           MFSD+F + +P +IS +W+ WGIELLV ANF+FQ+ILT+NGSRRRHTPGYKLSLTVWFSY
Sbjct: 1   MFSDLFLRILPGHISFIWNNWGIELLVFANFMFQVILTYNGSRRRHTPGYKLSLTVWFSY 60

Query: 61  LLAAKIATVVLGKLTTIEIGKDQRNTHTQIQALLAPLMFMQIGNPDTITAYSIEDNQLGV 120
           LLAAKIATVVLGKLTTI+IG ++RNTHTQ+QALLAPLMFMQIGNPDTITAYSIEDNQLGV
Sbjct: 61  LLAAKIATVVLGKLTTIDIGHERRNTHTQVQALLAPLMFMQIGNPDTITAYSIEDNQLGV 120

Query: 121 RQVFSMVIQVAIMFYILIRSWTPSTTSFLYLPMSLAGIIKYGETSWALKSALNGNFGFTI 180
           RQVFSMVIQV IMFYIL+RSWT S TSFLYLPMSLAGIIKYGETSWALKSAL+GN GFTI
Sbjct: 121 RQVFSMVIQVTIMFYILVRSWTDSKTSFLYLPMSLAGIIKYGETSWALKSALSGNLGFTI 180

Query: 181 ADFFKYHEIARLFDKLPQGENELPEARLILRAYYRFCCLKPHLENWLYYPPTDCENEKLY 240
           ADFFKYHE+A LFDKLPQ  NELPEA+LILRAYYRFCCLKPHLENWLYYPPTDC+++KLY
Sbjct: 181 ADFFKYHEVANLFDKLPQ--NELPEAKLILRAYYRFCCLKPHLENWLYYPPTDCDHKKLY 240

Query: 241 IKDCEYEDVFRITDSELGFMYDALYTKAPVVYTRKGLILRFISLLSLIATLVGFSVLFKD 300
           I DCEYEDVFRITDSELGFMYDALYTKAPV+YTRKGLILRFISLLSLIATL GFSVLFKD
Sbjct: 241 IDDCEYEDVFRITDSELGFMYDALYTKAPVIYTRKGLILRFISLLSLIATLCGFSVLFKD 300

Query: 301 AFVYNISAGFINFVLIASLIIEVYQILRLPFTDWAIIQMIRHYETFPFLLGFLKSLAPQS 360
           AFVYNIS GFI++VLIASLIIE+YQI+R+PFTDWAI+QMIRH+ETFP L G L SLAPQS
Sbjct: 301 AFVYNISVGFIHYVLIASLIIEIYQIMRIPFTDWAIVQMIRHHETFPILRGLLHSLAPQS 360

Query: 361 ATWRRWSNTMGQFNLLDFCLQTKHRNYSRIKILRYWGMDMKLRKQLSLDRVEVHPKVKEL 420
           ATWRRWSNTMGQFNLLDFCLQTKHRNYSRIK+LR WG+DMKLRKQ+SLDR++VHP+VKEL
Sbjct: 361 ATWRRWSNTMGQFNLLDFCLQTKHRNYSRIKLLRTWGLDMKLRKQMSLDRIDVHPEVKEL 420

Query: 421 VVTELREIDTIKGLEEFDQRGQWTIQRYRGKLKLSDESSLIQALETTVQKRPFDKSIFIW 480
           VVTELREI+ IKG EEF+QRGQWTI RY+ KLK +D+S LI+ALETTV KRPFDKSIFIW
Sbjct: 421 VVTELREIEKIKGQEEFEQRGQWTIDRYKTKLKQNDDSILIKALETTVNKRPFDKSIFIW 480

Query: 481 HITTNIFYHIRRFHDTTEVTKMEAIMNLSDYMMYLLVTRSHVLSSTTGDIIFDHSCVKLG 540
           HITTNIFYHIRRFHDT+   KMEAIM++S+YMMYLLVTRSHVLS+TTG+IIFDHSCVKLG
Sbjct: 481 HITTNIFYHIRRFHDTSIGNKMEAIMSISNYMMYLLVTRSHVLSTTTGEIIFDHSCVKLG 540

Query: 541 RFTRTGRLNKEDACKELLELQKEGMFEVKGPNEPPESEAEKVVVGNWNLLKDVKELADSL 600
           +FTRTG LNKEDAC  LL LQKEG  + + P+EP  SEAEKVVVGNW+LLKDVK+LADSL
Sbjct: 541 KFTRTGHLNKEDACNALLNLQKEGTLQAEEPHEP--SEAEKVVVGNWHLLKDVKDLADSL 600

Query: 601 STLSKENIWKIIGSMWVEMLGYAASHCEMEYHSEHIRQGGELITHVWLLVAHNVTKYSSY 660
             LS EN W++IGSMW EMLGYAAS CEMEYHSEHIRQGGELITHVWLL+AHNVTKYSSY
Sbjct: 601 LFLSNENRWQLIGSMWFEMLGYAASKCEMEYHSEHIRQGGELITHVWLLIAHNVTKYSSY 660

Query: 661 EYHLSSQDEETPTTSR 677
           EYH+  QDEE    SR
Sbjct: 661 EYHVGGQDEEIAANSR 672

BLAST of Tan0016743 vs. ExPASy TrEMBL
Match: A0A1S4DZ97 (uncharacterized protein LOC103493508 OS=Cucumis melo OX=3656 GN=LOC103493508 PE=4 SV=1)

HSP 1 Score: 1144.4 bits (2959), Expect = 0.0e+00
Identity = 558/675 (82.67%), Postives = 611/675 (90.52%), Query Frame = 0

Query: 1   MFSDIFEKFIPRNISSLWSYWGIELLVLANFLFQIILTFNGSRRRHTPGYKLSLTVWFSY 60
           MFS++F+  +P +IS +W YWGIELLVLANF+FQ+ILTFNGSRRRHTPG +LSL VWFSY
Sbjct: 1   MFSELFDLIVPEHISFIWGYWGIELLVLANFVFQVILTFNGSRRRHTPGNRLSLIVWFSY 60

Query: 61  LLAAKIATVVLGKLTTIEIGKDQRNTHTQIQALLAPLMFMQIGNPDTITAYSIEDNQLGV 120
           LLAAKIATVVLGKLTTI+IG++QRNTHTQ+QALLAPLMFMQIGNPDTITAYSIEDNQLGV
Sbjct: 61  LLAAKIATVVLGKLTTIDIGREQRNTHTQVQALLAPLMFMQIGNPDTITAYSIEDNQLGV 120

Query: 121 RQVFSMVIQVAIMFYILIRSWTPSTTSFLYLPMSLAGIIKYGETSWALKSALNGNFGFTI 180
           RQVFSMVIQV IMFYIL+RSWT S TSFLYLPMSLAGIIKYGETSWALKSALNGNFGFTI
Sbjct: 121 RQVFSMVIQVGIMFYILVRSWTDSKTSFLYLPMSLAGIIKYGETSWALKSALNGNFGFTI 180

Query: 181 ADFFKYHEIARLFDKLPQGENELPEARLILRAYYRFCCLKPHLENWLYYPPTDCENEKLY 240
           ADFFKYHE+A LF+KLPQGENELPEA LILRAYYRFCCLKPHLENWLYYPPTDC+ +KLY
Sbjct: 181 ADFFKYHEVANLFNKLPQGENELPEANLILRAYYRFCCLKPHLENWLYYPPTDCDQDKLY 240

Query: 241 IKDCEYEDVFRITDSELGFMYDALYTKAPVVYTRKGLILRFISLLSLIATLVGFSVLFKD 300
           IKDC YEDVFRITD ELGFMYDALYTKAPVVYTRKGLILRFISLLS+IATL GFSVLFKD
Sbjct: 241 IKDCGYEDVFRITDVELGFMYDALYTKAPVVYTRKGLILRFISLLSVIATLCGFSVLFKD 300

Query: 301 AFVYNISAGFINFVLIASLIIEVYQILRLPFTDWAIIQMIRHYETFPFLLGFLKSLAPQS 360
           AFVYNIS GFI+FVLIA+LIIE+YQILRLPFTDWAI+QM+RH+E FP L GFL+SLAPQS
Sbjct: 301 AFVYNISIGFIHFVLIAALIIEIYQILRLPFTDWAIVQMVRHHEAFPILRGFLRSLAPQS 360

Query: 361 ATWRRWSNTMGQFNLLDFCLQTKHRNYSRIKILRYWGMDMKLRKQLSLDRVEVHPKVKEL 420
           ATWRRWSNTMGQFNLLDFCLQTKHRNYSRIKILRYWGMDMKLRKQLSLDR++VHP+V+E 
Sbjct: 361 ATWRRWSNTMGQFNLLDFCLQTKHRNYSRIKILRYWGMDMKLRKQLSLDRIDVHPEVREF 420

Query: 421 VVTELREIDTIKGLEEFDQRGQWTIQRYRGKLKLSDESSLIQALETTVQKRPFDKSIFIW 480
           +VTELREI+ IKG EEFD RGQWTI RY+ K     E+ LI+A+ETTV KRPFDK IFIW
Sbjct: 421 LVTELREIEEIKGQEEFDNRGQWTIDRYKTKF----ENKLIKAIETTVLKRPFDKCIFIW 480

Query: 481 HITTNIFYHIRRFHDTTEVTKMEAIMNLSDYMMYLLVTRSHVLSSTTGDIIFDHSCVKLG 540
           HITTNIFY+I  + D +   K EAIM LSDYMMYL+VTRSHVLS+TT DIIFDHSCVKLG
Sbjct: 481 HITTNIFYNIEGYRDNSVGNKTEAIMCLSDYMMYLVVTRSHVLSTTTADIIFDHSCVKLG 540

Query: 541 RFTRTGRLNKEDACKELLELQKEGMFEVKGPNEPPESEAEKVVVGNWNLLKDVKELADSL 600
           +FTRTGRL KED C ++L L+KE +   + P+EP ESEAEKVVVGNW+L+KDVKELAD L
Sbjct: 541 KFTRTGRLKKEDICNDILNLKKESILHAREPHEPIESEAEKVVVGNWHLMKDVKELADCL 600

Query: 601 STLSKENIWKIIGSMWVEMLGYAASHCEMEYHSEHIRQGGELITHVWLLVAHNVTKYSSY 660
            TLS E+ WK+IGSMW EMLGYAAS CEMEYHSEHIRQGGELITHVWLL+AHNVTKYSSY
Sbjct: 601 LTLSNEDKWKLIGSMWFEMLGYAASKCEMEYHSEHIRQGGELITHVWLLIAHNVTKYSSY 660

Query: 661 EYHLSSQDEETPTTS 676
           EYH   QDEETP TS
Sbjct: 661 EYHAGGQDEETPATS 671

BLAST of Tan0016743 vs. ExPASy TrEMBL
Match: A0A5A7TID1 (DUF4220 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold546G00210 PE=4 SV=1)

HSP 1 Score: 1144.4 bits (2959), Expect = 0.0e+00
Identity = 558/675 (82.67%), Postives = 611/675 (90.52%), Query Frame = 0

Query: 1   MFSDIFEKFIPRNISSLWSYWGIELLVLANFLFQIILTFNGSRRRHTPGYKLSLTVWFSY 60
           MFS++F+  +P +IS +W YWGIELLVLANF+FQ+ILTFNGSRRRHTPG +LSL VWFSY
Sbjct: 1   MFSELFDLIVPEHISFIWGYWGIELLVLANFVFQVILTFNGSRRRHTPGNRLSLIVWFSY 60

Query: 61  LLAAKIATVVLGKLTTIEIGKDQRNTHTQIQALLAPLMFMQIGNPDTITAYSIEDNQLGV 120
           LLAAKIATVVLGKLTTI+IG++QRNTHTQ+QALLAPLMFMQIGNPDTITAYSIEDNQLGV
Sbjct: 61  LLAAKIATVVLGKLTTIDIGREQRNTHTQVQALLAPLMFMQIGNPDTITAYSIEDNQLGV 120

Query: 121 RQVFSMVIQVAIMFYILIRSWTPSTTSFLYLPMSLAGIIKYGETSWALKSALNGNFGFTI 180
           RQVFSMVIQV IMFYIL+RSWT S TSFLYLPMSLAGIIKYGETSWALKSALNGNFGFTI
Sbjct: 121 RQVFSMVIQVGIMFYILVRSWTDSKTSFLYLPMSLAGIIKYGETSWALKSALNGNFGFTI 180

Query: 181 ADFFKYHEIARLFDKLPQGENELPEARLILRAYYRFCCLKPHLENWLYYPPTDCENEKLY 240
           ADFFKYHE+A LF+KLPQGENELPEA LILRAYYRFCCLKPHLENWLYYPPTDC+ +KLY
Sbjct: 181 ADFFKYHEVANLFNKLPQGENELPEANLILRAYYRFCCLKPHLENWLYYPPTDCDQDKLY 240

Query: 241 IKDCEYEDVFRITDSELGFMYDALYTKAPVVYTRKGLILRFISLLSLIATLVGFSVLFKD 300
           IKDC YEDVFRITD ELGFMYDALYTKAPVVYTRKGLILRFISLLS+IATL GFSVLFKD
Sbjct: 241 IKDCGYEDVFRITDVELGFMYDALYTKAPVVYTRKGLILRFISLLSVIATLCGFSVLFKD 300

Query: 301 AFVYNISAGFINFVLIASLIIEVYQILRLPFTDWAIIQMIRHYETFPFLLGFLKSLAPQS 360
           AFVYNIS GFI+FVLIA+LIIE+YQILRLPFTDWAI+QM+RH+E FP L GFL+SLAPQS
Sbjct: 301 AFVYNISIGFIHFVLIAALIIEIYQILRLPFTDWAIVQMVRHHEAFPILRGFLRSLAPQS 360

Query: 361 ATWRRWSNTMGQFNLLDFCLQTKHRNYSRIKILRYWGMDMKLRKQLSLDRVEVHPKVKEL 420
           ATWRRWSNTMGQFNLLDFCLQTKHRNYSRIKILRYWGMDMKLRKQLSLDR++VHP+V+E 
Sbjct: 361 ATWRRWSNTMGQFNLLDFCLQTKHRNYSRIKILRYWGMDMKLRKQLSLDRIDVHPEVREF 420

Query: 421 VVTELREIDTIKGLEEFDQRGQWTIQRYRGKLKLSDESSLIQALETTVQKRPFDKSIFIW 480
           +VTELREI+ IKG EEFD RGQWTI RY+ K     E+ LI+A+ETTV KRPFDK IFIW
Sbjct: 421 LVTELREIEEIKGQEEFDNRGQWTIDRYKTKF----ENKLIKAIETTVLKRPFDKCIFIW 480

Query: 481 HITTNIFYHIRRFHDTTEVTKMEAIMNLSDYMMYLLVTRSHVLSSTTGDIIFDHSCVKLG 540
           HITTNIFY+I  + D +   K EAIM LSDYMMYL+VTRSHVLS+TT DIIFDHSCVKLG
Sbjct: 481 HITTNIFYNIEGYRDNSVGNKTEAIMCLSDYMMYLVVTRSHVLSTTTADIIFDHSCVKLG 540

Query: 541 RFTRTGRLNKEDACKELLELQKEGMFEVKGPNEPPESEAEKVVVGNWNLLKDVKELADSL 600
           +FTRTGRL KED C ++L L+KE +   + P+EP ESEAEKVVVGNW+L+KDVKELAD L
Sbjct: 541 KFTRTGRLKKEDICNDILNLKKESILHAREPHEPIESEAEKVVVGNWHLMKDVKELADCL 600

Query: 601 STLSKENIWKIIGSMWVEMLGYAASHCEMEYHSEHIRQGGELITHVWLLVAHNVTKYSSY 660
            TLS E+ WK+IGSMW EMLGYAAS CEMEYHSEHIRQGGELITHVWLL+AHNVTKYSSY
Sbjct: 601 LTLSNEDKWKLIGSMWFEMLGYAASKCEMEYHSEHIRQGGELITHVWLLIAHNVTKYSSY 660

Query: 661 EYHLSSQDEETPTTS 676
           EYH   QDEETP TS
Sbjct: 661 EYHAGGQDEETPATS 671

BLAST of Tan0016743 vs. TAIR 10
Match: AT5G45540.1 (Protein of unknown function (DUF594) )

HSP 1 Score: 196.8 bits (499), Expect = 5.5e-50
Identity = 201/794 (25.31%), Postives = 335/794 (42.19%), Query Frame = 0

Query: 10  IPRNISSLWSYWGIELLVLANFLFQIILTFNGSRRRHTPGYKLSLTVWFSYLLAAKIATV 69
           IP ++  LW  W I  +++ +   Q IL F    RR T      + +W +YLLA   A  
Sbjct: 5   IPPHLRKLWDKWNIRGVIILSLFLQTILIFFAPSRRRTAKKLFLVLIWSAYLLADWAADY 64

Query: 70  VLGKLTTIEIGKDQRNTHTQIQALLA---PLMFMQIGNPDTITAYSIEDNQLGVRQVFSM 129
            +G+++  +  + + N  ++ + LLA   P + + +G PDTITA ++EDN+L  R +FS+
Sbjct: 65  AVGQISDSQEEEAESNKPSKNRELLAFWSPFLLLHLGGPDTITALALEDNELWDRHLFSL 124

Query: 130 VIQVAIMFYILIRSWTPSTTSFLYLPMSLAGIIKYGETSWALKSALNGNF---------- 189
           V Q     Y+++ S  P+      L M + G+IKY E + AL SA    F          
Sbjct: 125 VCQAVATVYVILLS-IPNRLLTPTLIMFVGGVIKYVERTAALFSASLDKFKDSMLDDPDP 184

Query: 190 GFTIADFFKYHEIARLFDKLP-------------------QGENELPEARLILRAYYRFC 249
           G   A   + +E AR    +P                   + +NEL   ++I  AY  F 
Sbjct: 185 GANYAKLMEEYE-ARKKMNMPTDVIVVKDPEKGREGNTPVRPDNELTALQVIQYAYKYFN 244

Query: 250 CLKPHLENWLYYPPTDCENEKLYIKDCEYEDVFRITDSELGFMYDALYTKAPVVYTRKGL 309
             K  + + ++      E+ K + K    E+  RI + ELG +YD L+TKA +++   G 
Sbjct: 245 IFKGLIVDLIFTNQERDESRKFFDK-LTAEEALRIIEVELGLIYDCLFTKAEILHNWTGA 304

Query: 310 ILRFISLLSLIATLVGFSVLFKDAFVYNISAGFINFVLIASLIIEVYQILRLPFTDWAII 369
           + RFI+L  L+A+L  F +  KD +           +LI  + ++   +L    +DW I 
Sbjct: 305 VFRFIALGCLVASLCLFKMNKKDQY-DGFDVVLTYALLICGIALDSIALLMFCVSDWTIA 364

Query: 370 QMIRHYE-----------TFPFLLGFL-------------KSLAPQSATWRRWSNTMGQF 429
           ++ +  E              ++L F                +  ++  +RRWS  +  +
Sbjct: 365 RLRKLKEDLEEKDTLTDRVLNWILDFKTLRWKRSKCSQDGHQVLNRNFMFRRWSEYVHAY 424

Query: 430 NLLDFCL--QTKHRNYSRIKILRYWGMDMKLRKQLSLDRV-------------------- 489
           NL+ FCL  + K  +Y++ KI  ++   + +   LS+D                      
Sbjct: 425 NLIGFCLGIRPKRIHYTKGKIHSFFHQTVHI---LSIDTAIENATRGTRQFHNWIGRFLS 484

Query: 490 ------------------------------------------EVHPKVKELVVTELREI- 549
                                                     E+   V + +  EL E  
Sbjct: 485 NLSKRDNSVIRTGLRWFLFFPQLLGLLIYNFLDFFGIKDLVEEIRFTVSDRLTRELWEFI 544

Query: 550 --------------DTIKGLEEFDQRGQWTIQRYRGKLKL--SDESSLIQALETTVQKRP 609
                         ++ KG+     RG WT+     K K   +D + L+Q     V ++ 
Sbjct: 545 FTEVQQKHRFAEDQESAKGIS--SARGNWTLLETSSKKKEDGTDHTKLLQ----YVTEKD 604

Query: 610 FDKSIFIWHITTNIFYH--------IRRFHDTT----EVTKMEAIMNLSDYMMYLLVTRS 653
           +D+SI +WHI T + Y          +  H T     E +  E    LSDYMMYLL+ + 
Sbjct: 605 YDQSILLWHIATELLYQKPIDKKVTEKEEHSTNREKEEHSNREFSKILSDYMMYLLIVQP 664

BLAST of Tan0016743 vs. TAIR 10
Match: AT5G45530.1 (Protein of unknown function (DUF594) )

HSP 1 Score: 168.3 bits (425), Expect = 2.1e-41
Identity = 191/794 (24.06%), Postives = 340/794 (42.82%), Query Frame = 0

Query: 10  IPRNISSLWSYWGIELLVLANFLFQIILTFNGSRRRHTPGYKLSLTVWFSYLLAAKIATV 69
           IP  I  +   W I  LV+ + LFQ  L F    R+ T    L+  +W +YLLA   A  
Sbjct: 4   IPPAIKKILDKWNIRGLVIMSLLFQTSLIFLAPMRKRTSKKLLAAVLWTAYLLADWTANY 63

Query: 70  VLGKLT-----TIEIGKDQRNTHTQIQALLAPLMFMQIGNPDTITAYSIEDNQLGVRQVF 129
            + ++T       E G   +N   ++ AL AP + + +G PDTITA ++EDN L  R +F
Sbjct: 64  AVSQITKNQGKETEPGDPPKN--KKLLALWAPFLLLHLGGPDTITALALEDNALWQRHLF 123

Query: 130 SMVIQVAIMFYILIRS-----WTPSTTSFLYLPMSLAGIIKYGETSWALKSALNGNFGFT 189
            +V Q     Y +++S     W P T  F      + G IKY E + AL SA    F   
Sbjct: 124 GLVSQALAGVYAVVQSLENVLWPPITLLF------ITGTIKYVERTRALYSASLDKFKDR 183

Query: 190 I-------ADFFKYHE--IARLFDKLP-------------------QGENELPEARLILR 249
           +       +++ K  E   +R    LP                   + + +L +  ++  
Sbjct: 184 MLQRADAGSNYAKLMEEFASRKMSNLPTEIFLTDEPDKHERPPTLVKPDRDLTDLEIVQY 243

Query: 250 AYYRFCCLKPHLENWLYYPPTDCENEKLYIKDCEYEDVFRITDSELGFMYDALYTKAPVV 309
            +  F   K  + + L +   + +  + + K+ +  +  RI ++ELGF+Y+++YTK  ++
Sbjct: 244 GFKFFNTFKGLVVD-LIFSFRERDESRDFFKELKPGEALRIIETELGFLYESMYTKTAIL 303

Query: 310 YTRKGLILRFISLLSLIATLVGFSVLFKDAFVYNISAGFINFVL-IASLIIEVYQILRLP 369
           +T  G + R IS  SL+++   F      +  ++ +   I +VL I  + +++  ++   
Sbjct: 304 HTGIGTLFRLISFGSLLSSFFVFHRRPLKSEDFHGADVVITYVLFIVGIALDLASMVIFL 363

Query: 370 FTDW--AIIQMIRH---------YETFPFLLGFLK--------------SLAPQSATWRR 429
            +DW  A+++ ++             F + L F K               +       RR
Sbjct: 364 LSDWTFAVLRNLKDDPEEKSTSIDSLFNWFLEFRKPRWKKHTCNGNQTHEVLSTGFFTRR 423

Query: 430 WSNTMGQFNLLDFCLQTK------HRNYS----------------RIKILRYW--GMDMK 489
           WS T+  FN + FCL+ K       RN +                RI+++  W   ++  
Sbjct: 424 WSGTIYGFNFIGFCLKAKVSRIHQKRNCNLLVWDYVVSLFDLVIRRIQMMIGWIKNVNRS 483

Query: 490 LR-------KQLSLDRVEVHP-------KVKELVVTELREIDTIKGLEEFDQ-------- 549
           +R       K+  + R  V+P        + E+     + ID I  +  +          
Sbjct: 484 IRSVLRQWSKKNPMIRCTVYPLYLVFFAGIPEVFRVLWKYIDRIFSVTSYLDGIRFISRE 543

Query: 550 ---RGQW---------------------TIQRYRGKLKLSDESSL-IQALETTVQKRPFD 609
              + QW                      +   RG+  L D   + +  L   ++K  +D
Sbjct: 544 PLTKNQWEFIFNEVKDKSGFAETPEVAKKVSWARGEWALRDSKLMEVDTLMRYIEKVDYD 603

Query: 610 KSIFIWHITTNIFYH----------IRRFHDTTEVTKMEAIMNLSDYMMYLLVTRSHVLS 653
           +S+ +WHI T + +            R  +D  E +K+     +SDYMMYLL+ R  ++S
Sbjct: 604 QSLLLWHIATELCFQKEEGGKMEKLSREGYDDREFSKI-----ISDYMMYLLIMRPKLMS 663

BLAST of Tan0016743 vs. TAIR 10
Match: AT5G45470.1 (Protein of unknown function (DUF594) )

HSP 1 Score: 157.1 bits (396), Expect = 4.8e-38
Identity = 201/857 (23.45%), Postives = 337/857 (39.32%), Query Frame = 0

Query: 10  IPRNISSLWSYWGIELLVLANFLFQIILTFNGSRRRHTPGYKLSLTVWFSYLLA---AKI 69
           IP++I  +W  W I   V+ +   Q IL      R+ TP   L + VW SYLLA   A  
Sbjct: 5   IPKHIKDVWDRWNIRGAVILSLTLQAILICFSPLRKRTPRRLLIVLVWSSYLLADWSANF 64

Query: 70  ATVVLGKLTTIEIGKDQRNTHTQIQALLAPLMFMQIGNPDTITAYSIEDNQLGVRQVFSM 129
           A  ++ K    ++  D      ++ AL AP + + +G PDTITA+++EDN L +R VF +
Sbjct: 65  AVGLISKNQGKDLKPDDPPQDKKVMALWAPFLLLHLGGPDTITAFALEDNALWLRHVFGL 124

Query: 130 VIQVAIMFYILIRSWTPSTTSFLYLPMSLAGIIKYGETSWALKSALNGNFGFT------- 189
           V Q     Y+++ S  P++   + + + ++G IKY E + AL SA    F  +       
Sbjct: 125 VFQAIAGVYVVVMS-LPNSLWVVIVLVFVSGTIKYLERTTALYSASLDKFRDSMIQAPDP 184

Query: 190 ------IADFFKYHEIARLFDKL-----PQGEN-----------------ELPEARLILR 249
                 + + +K  + ARL  K+     P  EN                 +L +  ++  
Sbjct: 185 GPNYAKLMEEYKAKKEARLPTKIVLIDEPDKENRPKKLEHPALASKKRKKDLTDLEIVQY 244

Query: 250 AYYRFCCLKPHLENWLYYPPTDCENEKLYIKDCEYEDVFRITDSELGFMYDALYTKAPVV 309
           AY  F   K  + N ++      E+ +++    + E+  RI + ELGF+YDAL+TK  ++
Sbjct: 245 AYKFFNTFKGLVVNLIFSFRERDESLEIFENLNDPEEALRIIEIELGFLYDALFTKIAIL 304

Query: 310 YTRKGLILRFISLLSLIATLVGFSVLFKDAFVYNISAGFINFVLIA-SLIIEVYQILRLP 369
           +T  G + R  +  +L+A  + F         ++ +   + + L A  L+++   IL   
Sbjct: 305 HTGIGTVSRVFASGTLVAAFIIFHKKPNKGTDFHGADVVVTYTLFAVGLVLDFISILLFL 364

Query: 370 FTDW--AIIQMIR---------HYETFPFLLGFLK-SLAPQSA----------------- 429
           F+DW  A    ++             F +LL F K    PQ                   
Sbjct: 365 FSDWTCAAYSSLKDDPDELQSWQERCFNWLLKFRKLRWTPQECHKTGMHKCTKEGLKPCI 424

Query: 430 --------------------------------------------TWRRWSNTMGQFNLLD 489
                                                         RRWS ++  FN + 
Sbjct: 425 MKGADKKEGGNKNEGGNKKEGDDKKEAADKCSLVDKHDVLTTRFFLRRWSGSINVFNFIA 484

Query: 490 FCLQT----------KHRNYS-----------RIKILRYWGMDMKL-------------- 549
           +  +             R YS              I + +G  +KL              
Sbjct: 485 YATKADVERIHDARGSFRRYSWKIITFPFKKLNFVIKKIFGSIVKLINEVHRRISHEVNA 544

Query: 550 --RKQL------------------------------------SLDRVE----VH--PKVK 609
             RK L                                    +LD V     VH  P  +
Sbjct: 545 LSRKHLWARRFLYPIYFEFISRIPHFIKSVWDILSEFFDISDTLDMVHKTLFVHGEPMTR 604

Query: 610 EL---VVTELREI----DTIKGLEEFD-QRGQWTIQRYRGKLKLSDESSLIQALETTVQK 653
           EL   +  EL+      D+ +  +     RG+WT+   R  L +  E    + L   V K
Sbjct: 605 ELWKFIFEELKNKSKYGDSPENAKRISLARGEWTL---RENLPVDAER---EKLVRYVTK 664

BLAST of Tan0016743 vs. TAIR 10
Match: AT4G19090.1 (Protein of unknown function (DUF594) )

HSP 1 Score: 124.0 bits (310), Expect = 4.5e-28
Identity = 161/702 (22.93%), Postives = 279/702 (39.74%), Query Frame = 0

Query: 82  DQRNTHTQIQALLAPLMFMQIGNPDTITAYSIEDNQLGVRQVFSMVIQVAIMFYILIRSW 141
           D    + ++ AL AP + + +G PDTITA+S+EDN L  R    +V Q     Y++++S 
Sbjct: 74  DDPPQNKKLVALWAPFLLLHLGGPDTITAFSLEDNALWNRHFLGLVFQALAGVYVVVQS- 133

Query: 142 TPSTTSFLYLPMSLAGIIKYGETSWALKSALNGNFGFTIADF----FKYHEIARLFDKLP 201
            P+  S + L + +AG  KY E + AL  A +  +  ++       F Y +  R  D   
Sbjct: 134 LPNVLSVIILLLFIAGTSKYLERTIALYLASSDKYRNSMLQASNSRFDYTDQTRDLDMDT 193

Query: 202 QGENEL--------PEARLILRAYYRFCCLKPHLENWLYYPPTDCENE-KLYIKDCEYED 261
           +  +E+        P+   +L+ +        HLE   Y    +  +E K +    + +D
Sbjct: 194 KLASEMNMKEHRGQPKPLKLLQPHKEL----THLEILQYAFFLELRDESKAFFSALQLKD 253

Query: 262 -VFRITDSELGFMYDALYTKAPVVYTRKGLILRFISLLSLIATLVGFSVLFKDAFVYNIS 321
             F I ++EL F+Y+ LYTK  V+++  GL+ RFISL SL++    +         ++ +
Sbjct: 254 EAFCIIEAELDFIYEGLYTKGSVLHSWVGLVSRFISLGSLLSAFTIYHYRHNKIQEFHKA 313

Query: 322 AGFINFVL-IASLIIEVYQILRLPFTDW--AIIQMIR-----HYETFPFLLGFLKSLAPQ 381
              I + L +  + ++V  I     +DW  AI+  ++      Y     +L ++  L   
Sbjct: 314 DIVITYTLFLVGIALDVISIHMFMVSDWTTAILAKLKDDPDERYSGKDHILNWILFLKRP 373

Query: 382 SATW------------------RRWSNTMGQFNLLDF----------------------- 441
              W                  RRW+ ++   N L +                       
Sbjct: 374 KWKWQTCREGDQQEVLNTPFLLRRWTGSITMLNFLTYSMKADTERIHDPRSRACQLLWKI 433

Query: 442 ----------CLQTKHRNYSRIKILRYW-----------GMDMKLRKQLSLDR--VEVHP 501
                      +QT   N ++     +W           G +   R  + + +  VE   
Sbjct: 434 AVCPFSFIFSIIQTWFSNIAKWFCALHWYIIHHVIILPPGQNQVARSIIGIFKAFVEFWF 493

Query: 502 KVKELVVTELREIDTIKGLEEFDQRGQWTIQRYRGKLKLSDESSLIQALETTVQKR---- 561
            V    V  +  +    G+ +           Y  +L L   S ++ +LE T  K     
Sbjct: 494 NVPNFFVYLVTSVINFLGINDL---------FYEIRL-LQKNSQILYSLENTTTKSSARR 553

Query: 562 ------------------------PFDKSIFIWHITTNIFYHIR----------RFHDTT 621
                                    ++ S+ IWHI T + Y              +H   
Sbjct: 554 DWASSHTQIQIADHDMLLHYITDVDYEHSLLIWHIATELCYQEEDSAKENCDKSEYHTNR 613

Query: 622 EVTKMEAIMNLSDYMMYLLVTRSHVLSSTT--GDIIFDHSCVKLGRFTRTGRLNKEDACK 653
           +++K+     +SDYMMYLL+ +  ++S     G I F  +  +  RF +   + ++    
Sbjct: 614 KISKI-----ISDYMMYLLIMQPKLMSEVAGIGKIRFRDTLAEADRFFKKMGIIRD---S 673

BLAST of Tan0016743 vs. TAIR 10
Match: AT5G45460.1 (unknown protein; BEST Arabidopsis thaliana protein match is: Protein of unknown function (DUF594) (TAIR:AT5G45470.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 123.6 bits (309), Expect = 5.9e-28
Identity = 109/415 (26.27%), Postives = 187/415 (45.06%), Query Frame = 0

Query: 10  IPRNISSLWSYWGIELLVLANFLFQIILTFNGSRRRHTPGYKLSLTVWFSYLLA---AKI 69
           IP++I   W  W I   +  +   Q  L      R+ TP   L + +W SYLLA   A  
Sbjct: 5   IPKHIKDAWDRWNIRGTIFLSLTLQAFLICFSPLRKRTPRRHLIIVIWSSYLLADWSANF 64

Query: 70  ATVVLGKLTTIEIGKDQRNTHTQIQALLAPLMFMQIGNPDTITAYSIEDNQLGVRQVFSM 129
           A  ++ K    ++  D      ++ AL AP + + +G PDTITA+++EDN L +R VF +
Sbjct: 65  AVGLISKNQGKDLKPDDPPQDKKLMALWAPFLLLHLGGPDTITAFALEDNALWLRNVFGL 124

Query: 130 VIQVAIMFYILIRSWTPSTTSFLYLPMSLAGIIKYGETSWALKSALNGNFGFT------- 189
           V Q     Y++++S  P++     L + ++G IKY E + AL SA    F  +       
Sbjct: 125 VFQAIAGVYVVLQS-LPNSLWVTILLVFISGTIKYLERTTALYSASLDKFRDSMIQGPDP 184

Query: 190 ------IADFFKYHEIARLFDKL-----PQGEN-----------------ELPEARLILR 249
                 + + +K  + A+L  K+     P  E+                 EL    +   
Sbjct: 185 GPNYAKLMEEYKAKKEAKLPTKIILIDEPDKEHRPKKLEHPSLASETKRKELTHLEIAQY 244

Query: 250 AYYRFCCLKPHLENWLYYPPTDCENEKLYIKDCEYEDVFRITDSELGFMYDALYTKAPVV 309
           AY  F   K  + N ++      ++ +++    + E+  RI + ELGF+YDAL+TK  V+
Sbjct: 245 AYKFFNTFKGLVVNLIFSFRERDQSIEIFQNLEDPEEALRIIEIELGFLYDALFTKNAVL 304

Query: 310 YTRKGLILRFISLLSLIATLVGFSVLFKDAFVYNISAGFINFVLIA-SLIIEVYQILRLP 369
           +T  G + R ++  SL+A  + F  +      ++ +   I ++L A  L+++   IL   
Sbjct: 305 HTVLGTVSRVVASGSLVAAFIIFHKISNKGRDFHGADVVITYILFAVGLVLDFISILLFL 364

Query: 370 FTDWAIIQMIRHYETFPFLLGFLKSLAPQSATWR-RWSNTMGQFNLLDFCLQTKH 385
           F+DW               L  LK    +  +W+ R+ N + +F  L + +Q  H
Sbjct: 365 FSDWTCA-----------ALSSLKDDPDEPLSWKDRFFNCLLEFRKLRWKMQECH 407

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
XP_023538013.10.0e+0090.91uncharacterized protein LOC111798897 [Cucurbita pepo subsp. pepo][more]
XP_022969679.10.0e+0090.91uncharacterized protein LOC111468632 [Cucurbita maxima][more]
XP_022937890.10.0e+0090.76uncharacterized protein LOC111444144 [Cucurbita moschata] >KAG6586039.1 hypothet... [more]
KAG7020815.10.0e+0090.94hypothetical protein SDJN02_17503, partial [Cucurbita argyrosperma subsp. argyro... [more]
XP_038889477.10.0e+0083.70uncharacterized protein LOC120079385 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A6J1I3C00.0e+0090.91uncharacterized protein LOC111468632 OS=Cucurbita maxima OX=3661 GN=LOC111468632... [more]
A0A6J1FI300.0e+0090.76uncharacterized protein LOC111444144 OS=Cucurbita moschata OX=3662 GN=LOC1114441... [more]
A0A6J1KHN90.0e+0083.73uncharacterized protein LOC111493941 OS=Cucurbita maxima OX=3661 GN=LOC111493941... [more]
A0A1S4DZ970.0e+0082.67uncharacterized protein LOC103493508 OS=Cucumis melo OX=3656 GN=LOC103493508 PE=... [more]
A0A5A7TID10.0e+0082.67DUF4220 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
Match NameE-valueIdentityDescription
AT5G45540.15.5e-5025.31Protein of unknown function (DUF594) [more]
AT5G45530.12.1e-4124.06Protein of unknown function (DUF594) [more]
AT5G45470.14.8e-3823.45Protein of unknown function (DUF594) [more]
AT4G19090.14.5e-2822.93Protein of unknown function (DUF594) [more]
AT5G45460.15.9e-2826.27unknown protein; BEST Arabidopsis thaliana protein match is: Protein of unknown ... [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR025315Domain of unknown function DUF4220PFAMPF13968DUF4220coord: 57..377
e-value: 1.3E-50
score: 172.7
IPR007658Protein of unknown function DUF594PFAMPF04578DUF594coord: 604..652
e-value: 4.2E-19
score: 67.9
NoneNo IPR availablePANTHERPTHR31325:SF221DUF594 FAMILY PROTEINcoord: 36..645
NoneNo IPR availablePANTHERPTHR31325OS01G0798800 PROTEIN-RELATEDcoord: 36..645

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0016743.1Tan0016743.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane