Tan0016285 (gene) Snake gourd v1

Overview
NameTan0016285
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
Descriptionbasic 7S globulin 2-like
LocationLG06: 8214782 .. 8216410 (+)
RNA-Seq ExpressionTan0016285
SyntenyTan0016285
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AATGGTCAGTCGTGTGTTCTTCTTCTTCACTTTCTCCCTCATTTCTCTTCCTCCTCTCTCACTCCAAACAGCCTTCGTCGCTCCCATTCACAAAGACCACAACTCCCTTCTGTACTCCATCTCCGTCCACCTCAAAACTCCACTCCGGCCGGCCAACCTCCACCTCGACCTCGGCGGCGCCTTCTCCTGGATCGACTGCTACAACCATTACAATTCTTCCTCTTACCAACTCGTCAAATGCAATTCTCCTCTTTCCAATTCTTTGGACCACGGCATTTGCGGCTCCTGTATCCAAGCTCCAACTCCAATCTGCGCCAACGACACCATTTTCTCCTACCTCTACCCCGAGAAACCTTCCATTCGAGATCAATTCGTCGATTATGATCACCCCAAGCTCACCGATTCCGAGAATTTAATCACAGATGTTCTCGCTCTCTCCACCACCGACGGTTTACTCCGTCGATTCTCCAACATTCCTTTTGCCTGCGTCAAGACTGATTTCCTCCGAGGACTGGCTAAGAATGTAATCGGCCTGGCCGCGCTCGGCCGTGCCAACGAATCGATTCCGTCCATGATTAGCTCGAAGTTCAATAGCCCTAGAATCTTCGCGATTTGCTTATCGGGAACGAGATTGGGATCTGGTGTGGCCTTTTTCGGATCTAAAGGACCGTACAGATTTTCCCCCAATGTCGATCTCTCCAAATCCCTAATTTACACTCCATTGCTCTTCAATCCCCATAGTGCCTCGATTTACACTCATTGGTTGCCGTCTTACGAATATTACATCGGACTCACCGCCATCAGAATCAACGGCGAGGCCGTCCGGTTCAATACTTCTCTGTTGGCGTTTGAGCCGGTTCACGGCCGCGGCGGAGCGAAGATCAGCACCTCCACTACCTACGCGCTGCTGCAGAGCAGTATTTACAGAGCATTCGCGACGGCGTTCATGAAGGAAGCGGCGGTGTTGAACTTCACGTTGACGAACGCAGTGGAGCCGTTCGGCGTGTGCTATGCCGCGGAGAGCATTGCGGTGACGGCGACGGGACCGACGGCGCCGGTTTTGGATTTGGAGATGGAGAAGGGGAAAGTGGTGTGGAAATTGGGGGGTAGGAATTCGATGGTGAGGATTAAGAAGATGGGAGTGGATGCGTGGTGCTTGGGATTTATCAATGGCGGGGAGTTTCCAAGGACGCCGATCGTGATCGGAGGTCTGCAAATGGAGGATCATTTGTTGCAGTTTGATCTTGAAAATTACAGATTTGGATTCAGTTCTTCGGCGTTGCTGCAAGGGACTTCGTGTTCCCAGTTCAACTTCACTTCTGTCGAGAGTTTTCTATAATAATACGATGGTTAAAATGACGATGTTAATTGATCTTCTCAAACCAGTTGATTCCATCTCCTATTTCTACTGATGTTGAACTAAAATCAAATTACTACCTAGATCTAGATATTTCTAATGAAAAATATTAAATAGGTTAATTTACTAGTTTAGTTCATAAATTTTATGTTTGTATCGATTTAGTCTCTTAATTTTTAAAAGTATTAAATAGGTCCCTAAACTTTAAATTTTGTGTTTAATAAGTATATGAACTTTAAAAATATTTAATAAATCCTCCAACTTCAATTTTG

mRNA sequence

AATGGTCAGTCGTGTGTTCTTCTTCTTCACTTTCTCCCTCATTTCTCTTCCTCCTCTCTCACTCCAAACAGCCTTCGTCGCTCCCATTCACAAAGACCACAACTCCCTTCTGTACTCCATCTCCGTCCACCTCAAAACTCCACTCCGGCCGGCCAACCTCCACCTCGACCTCGGCGGCGCCTTCTCCTGGATCGACTGCTACAACCATTACAATTCTTCCTCTTACCAACTCGTCAAATGCAATTCTCCTCTTTCCAATTCTTTGGACCACGGCATTTGCGGCTCCTGTATCCAAGCTCCAACTCCAATCTGCGCCAACGACACCATTTTCTCCTACCTCTACCCCGAGAAACCTTCCATTCGAGATCAATTCGTCGATTATGATCACCCCAAGCTCACCGATTCCGAGAATTTAATCACAGATGTTCTCGCTCTCTCCACCACCGACGGTTTACTCCGTCGATTCTCCAACATTCCTTTTGCCTGCGTCAAGACTGATTTCCTCCGAGGACTGGCTAAGAATGTAATCGGCCTGGCCGCGCTCGGCCGTGCCAACGAATCGATTCCGTCCATGATTAGCTCGAAGTTCAATAGCCCTAGAATCTTCGCGATTTGCTTATCGGGAACGAGATTGGGATCTGGTGTGGCCTTTTTCGGATCTAAAGGACCGTACAGATTTTCCCCCAATGTCGATCTCTCCAAATCCCTAATTTACACTCCATTGCTCTTCAATCCCCATAGTGCCTCGATTTACACTCATTGGTTGCCGTCTTACGAATATTACATCGGACTCACCGCCATCAGAATCAACGGCGAGGCCGTCCGGTTCAATACTTCTCTGTTGGCGTTTGAGCCGGTTCACGGCCGCGGCGGAGCGAAGATCAGCACCTCCACTACCTACGCGCTGCTGCAGAGCAGTATTTACAGAGCATTCGCGACGGCGTTCATGAAGGAAGCGGCGGTGTTGAACTTCACGTTGACGAACGCAGTGGAGCCGTTCGGCGTGTGCTATGCCGCGGAGAGCATTGCGGTGACGGCGACGGGACCGACGGCGCCGGTTTTGGATTTGGAGATGGAGAAGGGGAAAGTGGTGTGGAAATTGGGGGGTAGGAATTCGATGGTGAGGATTAAGAAGATGGGAGTGGATGCGTGGTGCTTGGGATTTATCAATGGCGGGGAGTTTCCAAGGACGCCGATCGTGATCGGAGGTCTGCAAATGGAGGATCATTTGTTGCAGTTTGATCTTGAAAATTACAGATTTGGATTCAGTTCTTCGGCGTTGCTGCAAGGGACTTCGTGTTCCCAGTTCAACTTCACTTCTGTCGAGAGTTTTCTATAATAATACGATGGTTAAAATGACGATGTTAATTGATCTTCTCAAACCAGTTGATTCCATCTCCTATTTCTACTGATGTTGAACTAAAATCAAATTACTACCTAGATCTAGATATTTCTAATGAAAAATATTAAATAGGTTAATTTACTAGTTTAGTTCATAAATTTTATGTTTGTATCGATTTAGTCTCTTAATTTTTAAAAGTATTAAATAGGTCCCTAAACTTTAAATTTTGTGTTTAATAAGTATATGAACTTTAAAAATATTTAATAAATCCTCCAACTTCAATTTTG

Coding sequence (CDS)

ATGGTCAGTCGTGTGTTCTTCTTCTTCACTTTCTCCCTCATTTCTCTTCCTCCTCTCTCACTCCAAACAGCCTTCGTCGCTCCCATTCACAAAGACCACAACTCCCTTCTGTACTCCATCTCCGTCCACCTCAAAACTCCACTCCGGCCGGCCAACCTCCACCTCGACCTCGGCGGCGCCTTCTCCTGGATCGACTGCTACAACCATTACAATTCTTCCTCTTACCAACTCGTCAAATGCAATTCTCCTCTTTCCAATTCTTTGGACCACGGCATTTGCGGCTCCTGTATCCAAGCTCCAACTCCAATCTGCGCCAACGACACCATTTTCTCCTACCTCTACCCCGAGAAACCTTCCATTCGAGATCAATTCGTCGATTATGATCACCCCAAGCTCACCGATTCCGAGAATTTAATCACAGATGTTCTCGCTCTCTCCACCACCGACGGTTTACTCCGTCGATTCTCCAACATTCCTTTTGCCTGCGTCAAGACTGATTTCCTCCGAGGACTGGCTAAGAATGTAATCGGCCTGGCCGCGCTCGGCCGTGCCAACGAATCGATTCCGTCCATGATTAGCTCGAAGTTCAATAGCCCTAGAATCTTCGCGATTTGCTTATCGGGAACGAGATTGGGATCTGGTGTGGCCTTTTTCGGATCTAAAGGACCGTACAGATTTTCCCCCAATGTCGATCTCTCCAAATCCCTAATTTACACTCCATTGCTCTTCAATCCCCATAGTGCCTCGATTTACACTCATTGGTTGCCGTCTTACGAATATTACATCGGACTCACCGCCATCAGAATCAACGGCGAGGCCGTCCGGTTCAATACTTCTCTGTTGGCGTTTGAGCCGGTTCACGGCCGCGGCGGAGCGAAGATCAGCACCTCCACTACCTACGCGCTGCTGCAGAGCAGTATTTACAGAGCATTCGCGACGGCGTTCATGAAGGAAGCGGCGGTGTTGAACTTCACGTTGACGAACGCAGTGGAGCCGTTCGGCGTGTGCTATGCCGCGGAGAGCATTGCGGTGACGGCGACGGGACCGACGGCGCCGGTTTTGGATTTGGAGATGGAGAAGGGGAAAGTGGTGTGGAAATTGGGGGGTAGGAATTCGATGGTGAGGATTAAGAAGATGGGAGTGGATGCGTGGTGCTTGGGATTTATCAATGGCGGGGAGTTTCCAAGGACGCCGATCGTGATCGGAGGTCTGCAAATGGAGGATCATTTGTTGCAGTTTGATCTTGAAAATTACAGATTTGGATTCAGTTCTTCGGCGTTGCTGCAAGGGACTTCGTGTTCCCAGTTCAACTTCACTTCTGTCGAGAGTTTTCTATAA

Protein sequence

MVSRVFFFFTFSLISLPPLSLQTAFVAPIHKDHNSLLYSISVHLKTPLRPANLHLDLGGAFSWIDCYNHYNSSSYQLVKCNSPLSNSLDHGICGSCIQAPTPICANDTIFSYLYPEKPSIRDQFVDYDHPKLTDSENLITDVLALSTTDGLLRRFSNIPFACVKTDFLRGLAKNVIGLAALGRANESIPSMISSKFNSPRIFAICLSGTRLGSGVAFFGSKGPYRFSPNVDLSKSLIYTPLLFNPHSASIYTHWLPSYEYYIGLTAIRINGEAVRFNTSLLAFEPVHGRGGAKISTSTTYALLQSSIYRAFATAFMKEAAVLNFTLTNAVEPFGVCYAAESIAVTATGPTAPVLDLEMEKGKVVWKLGGRNSMVRIKKMGVDAWCLGFINGGEFPRTPIVIGGLQMEDHLLQFDLENYRFGFSSSALLQGTSCSQFNFTSVESFL
Homology
BLAST of Tan0016285 vs. ExPASy Swiss-Prot
Match: I1JNS6 (Probable aspartic proteinase GIP1 OS=Glycine max OX=3847 GN=GIP1 PE=1 SV=2)

HSP 1 Score: 337.0 bits (863), Expect = 3.2e-91
Identity = 184/419 (43.91%), Postives = 253/419 (60.38%), Query Frame = 0

Query: 20  SLQTAFVAPIHKDHNSLLYSISVHLKTPLRPANLHLDLGGAFSWIDCYNHYNSSSYQLVK 79
           + Q   +API KD  + LY++SV LKTPL+P  LHL LG + SW+ C + Y SSS   + 
Sbjct: 23  TFQIPLIAPISKDDTTQLYTLSVFLKTPLQPTKLHLHLGSSLSWVLCDSTYTSSSSHHIP 82

Query: 80  CNSPLSNSLDHGICGSCIQAPTPICANDTIFSYLYPEKPSIRDQFVDYDHPKLTDSENLI 139
           CN+PL NS            P+  C+N++    L+PE P  R+  +D            +
Sbjct: 83  CNTPLCNSF-----------PSNACSNNSSLCALFPENPVTRNTLLD----------TAL 142

Query: 140 TDVLALSTTD--GLLRRFSNIPFACVKTDFLRGLAKNVIGLAALGRANESIPSMISSKFN 199
            D LAL T D    L   S+  F+C     L+GLA N +GLA+LGR+N S+P+ IS+   
Sbjct: 143 IDSLALPTYDASSSLVLISDFIFSCATAHLLQGLAANALGLASLGRSNYSLPAQISTSLT 202

Query: 200 SPRIFAICLSGTRLGSGVAFFGS-KGPYRFSPNVDLSKSLIYTPLLFNPHSASIYT-HWL 259
           SPR F +CL  +   +G A F S    + FS  +DL+    YT L+ NP + ++ T +  
Sbjct: 203 SPRSFTLCLPASSANTGAAIFASTASSFLFSSKIDLT----YTQLIVNPVADTVVTDNPQ 262

Query: 260 PSYEYYIGLTAIRINGEAVRFNTSLLAFEPVHGRGGAKISTSTTYALLQSSIYRAFATAF 319
           PS EY+I LT+I+ING+ +  N+S+L  +   G GG KIST+  Y +L++SIYR F   F
Sbjct: 263 PSDEYFINLTSIKINGKPLYINSSILTVDQT-GFGGTKISTAEPYTVLETSIYRLFVQRF 322

Query: 320 MKEAAVLNFTLTNAVEPFGVCYAAESIAVTATGPTAPVLDLEMEKGKVVWKLGGRNSMVR 379
           + E++  N T+T AVEPFGVCY A  +  T  GP  P +DL M    V W++ G NSMVR
Sbjct: 323 VNESSAFNLTVTEAVEPFGVCYPAGDLTETRVGPAVPTVDLVMHSEDVFWRIFGGNSMVR 382

Query: 380 IKKMGVDAWCLGFINGGEFPRTPIVIGGLQMEDHLLQFDLENYRFGFSSSALLQGTSCS 435
           + K GVD WCLGF++GG   RTPIVIGG Q+ED+L+QFDL++ RFGF+S+ LLQ   CS
Sbjct: 383 VAKGGVDVWCLGFVDGGTRGRTPIVIGGHQLEDNLMQFDLDSNRFGFTSTLLLQDAKCS 415

BLAST of Tan0016285 vs. ExPASy Swiss-Prot
Match: P0DO21 (Probable aspartic proteinase GIP2 OS=Nicotiana benthamiana OX=4100 GN=GIP2 PE=1 SV=1)

HSP 1 Score: 290.4 bits (742), Expect = 3.4e-77
Identity = 173/423 (40.90%), Postives = 241/423 (56.97%), Query Frame = 0

Query: 26  VAPIHKDHNSLLYSISVHLKTPLRPANLHLDLGGAFSWIDCYNHYNSSSYQLVKCNSPLS 85
           + PI KD  +L Y   +  +TPL P +L LDLGG F W+DC   Y SS+Y+  +C S   
Sbjct: 35  ILPITKDALTLQYLTQIQQRTPLVPVSLTLDLGGQFLWVDCDQGYVSSTYRPARCRSAQC 94

Query: 86  NSLDHGI-CGSCIQAPTPICANDTIFSYLYPEKPSIRDQFVDYDHPKLTDSENLITDVLA 145
           +    G  CG C   P P C N+T    L P+    R             S  L +D + 
Sbjct: 95  SLAGAGSGCGQCFSPPKPGCNNNT--CGLLPDNTITR----------TATSGELASDTVQ 154

Query: 146 LSTTDGLL--RRFS--NIPFACVKTDFLRGLAKNVIGLAALGRANESIPSMISSKFNSPR 205
           + +++G    R  S  +  F C  T  L GLA  V G+A LGR   S+PS  S++F+ PR
Sbjct: 155 VQSSNGKNPGRHVSDKDFLFVCGSTFLLEGLASGVKGMAGLGRTRISLPSQFSAEFSFPR 214

Query: 206 IFAICLSGTRLGSGVAFFGSKGPYRFSPNVDLSKS-LIYTPLLFNPHS-ASIYTHWLPSY 265
            FA+CLS +    GV  FG  GPY F PN + + +   YTPL  NP S AS ++   PS 
Sbjct: 215 KFAVCLSSSTNSKGVVLFGD-GPYTFLPNREFANNDFSYTPLFINPVSTASAFSSREPSS 274

Query: 266 EYYIGLTAIRINGEAVRFNTSLLAFEPVHGRGGAKISTSTTYALLQSSIYRAFATAFMKE 325
           EY+IG+ +I+IN + V  NT+LL+ +   G GG KIST   Y +L++SIY A    F+KE
Sbjct: 275 EYFIGVKSIKINEKVVPINTTLLSIDN-QGVGGTKISTVNPYTILETSIYNAVTNFFVKE 334

Query: 326 AAVLNFTLTNAVEPFGVCYAAESIAVTATGPTAPVLDLEMEKGKVVWKLGGRNSMVRIKK 385
             ++N T   +V PF  C+ + +IA T  GP  P +DL ++   V W++ G NSMV++ +
Sbjct: 335 --LVNITRVASVAPFRACFDSRNIASTRVGPAVPSIDLVLQNENVFWRIFGANSMVQVSE 394

Query: 386 MGVDAWCLGFINGGEFPRTPIVIGGLQMEDHLLQFDLENYRFGFSSSALLQGTSCSQFNF 442
              +  CLGF++GG  PRT IV+GG  +ED+LLQFDL   R GF+SS L + T+C+ FNF
Sbjct: 395 ---NVLCLGFVDGGVSPRTSIVVGGYTIEDNLLQFDLARSRLGFTSSILFRQTTCANFNF 438

BLAST of Tan0016285 vs. ExPASy Swiss-Prot
Match: P82952 (Gamma conglutin 1 OS=Prunus dulcis OX=3755 GN=Cgamma1 PE=1 SV=2)

HSP 1 Score: 219.9 bits (559), Expect = 5.6e-56
Identity = 153/450 (34.00%), Postives = 226/450 (50.22%), Query Frame = 0

Query: 5   VFFFFTFSLISLPPLSLQTAFVAPI---------HKDHNSLLYSISVHLKTPLRPANLHL 64
           + FF + SLI L   + ++    PI          KD  + L+ + +H +TPL      +
Sbjct: 9   LLFFCSLSLIILTSSATKSQTHVPIRPNKLVLKVQKDRATNLHVVQIHKRTPLVQFPFVI 68

Query: 65  DLGGAFSWIDCYNHYNSSSYQLVKCNSPLSNSLDHGICGSCIQAPT-PICANDTIFSYLY 124
           DL G F  ++C N Y SS+Y+   C+S      +   C +C  + T P C   T    L 
Sbjct: 69  DLTGRFLSVNCENQYTSSTYKAPVCHSSQCARANSHTCRTCSSSKTRPGC--HTNACGLL 128

Query: 125 PEKPSIRDQFVDYDHPKLTDSENLITDVLALSTTDGL----LRRFSNIPFACVKTDFL-R 184
              P  +           +    L  DVL + +T G     +  + +  FAC  ++ L +
Sbjct: 129 TTNPVTQQ----------SAQGELAEDVLKIPSTQGSSPGPMVTYPHFLFACAPSNILQK 188

Query: 185 GLAKNVIGLAALGRANESIPSMISSKFNSPRIFAICLSGTRLGSGVAFFGSKGPYRFSPN 244
           GL KNV G+A LG +  S+P  ++S F  P  FA+CL+ +   +G  FFG +GPY   P 
Sbjct: 189 GLPKNVQGVAGLGHSPISLPYQLASHFGFPPKFAVCLTSSPGKNGAVFFG-EGPYFMKPG 248

Query: 245 VDLSKSLIYTPLLFNPHSASIYTHWLPSYEYYIGLTAIRINGEAVRFNTSLLAFEPVHGR 304
           +D+S+ L Y P                  EYY       IN ++ + N ++L   P  G 
Sbjct: 249 IDVSRQLTYAPFTIGQQG-----------EYY-------INVQSFKINNAMLPSIPKGGF 308

Query: 305 GGAKISTSTTYALLQSSIYRAFATAFMKEAAVLNFTLTNAVEPFGVCYAAESIAVTATGP 364
           GGA IST+T Y  LQ+ I+RA    FM +  +        V PFG C+ A  I  +  GP
Sbjct: 309 GGAMISTTTPYTTLQTPIFRALNQLFMNQ--LRGVPHVKPVAPFGACFDANRIPTSKMGP 368

Query: 365 TAPVLDLEME-KGKVVWKLGGRNSMVRIKKMGVDAWCLGFINGGEFPRTPIVIGGLQMED 424
           T P +DL ++ K  ++W++ G N+M++  + GV   CL F++GG  P+ PIVIG  Q+ED
Sbjct: 369 TVPSIDLVLDNKKNIMWRIFGANAMIQ-PRPGV--MCLAFVDGGMRPKAPIVIGTQQLED 422

Query: 425 HLLQFDLENYRFGFSSSALLQGTSCSQFNF 439
           +LLQFDL N R GFSSS L + T+C+ FNF
Sbjct: 429 NLLQFDLMNSRLGFSSSLLFRRTNCANFNF 422

BLAST of Tan0016285 vs. ExPASy Swiss-Prot
Match: Q9FSH9 (Gamma conglutin 1 OS=Lupinus albus OX=3870 GN=Cgamma PE=1 SV=1)

HSP 1 Score: 174.1 bits (440), Expect = 3.5e-42
Identity = 137/430 (31.86%), Postives = 205/430 (47.67%), Query Frame = 0

Query: 26  VAPIHKDHNSLLYSISVHLKTPLRPANLHLDLGGAFSWIDCYNHYNSSSYQLVKCNSPLS 85
           V PI +D ++ L+  ++  +TPL    + LDL G   W+ C  HY+SS+YQ   C+S   
Sbjct: 49  VLPIQQDASTKLHWGNILKRTPLMQVPVLLDLNGKHLWVTCSQHYSSSTYQAPFCHSTQC 108

Query: 86  NSLDHGICGSCIQAPT--PICANDTIFSYLYPEKPSIRDQFVDYDHPKLTDSENLITDVL 145
           +  +   C +C  + T  P C N+T    L    P  ++  +            L  DVL
Sbjct: 109 SRANTHQCFTCTDSTTSRPGCHNNT--CGLISSNPVTQESGLG----------ELAQDVL 168

Query: 146 ALSTTD----GLLRRFSNIPFACVKTDFL--RGLAKNVIGLAALGRANESIPSMISSKFN 205
           AL +T     G L +     F+C  T FL  +GL  NV G   LG A  S+P+ + S F 
Sbjct: 169 ALHSTHGSKLGSLVKIPQFLFSCAPT-FLTQKGLPNNVQGALGLGHAPISLPNQLFSHFG 228

Query: 206 SPRIFAICLSGTRLGSGVAFFGS----KGPYRFSPNVDLSKSLIYTPLLFNPHSASIYTH 265
             R F +CLS     +G   FG             ++D+   ++YTPL  +         
Sbjct: 229 LKRQFTMCLSSYPTSNGAILFGDINDPNNNNYIHNSLDVLHDMVYTPLTISKQG------ 288

Query: 266 WLPSYEYYIGLTAIRINGEAV-------RF-NTSLLAFEPVHGRGGAKISTSTTYALLQS 325
                EY+I ++AIR+N   V        F ++S  ++      GGA I+T+  Y +L+ 
Sbjct: 289 -----EYFIQVSAIRVNKHMVIPTKNPSMFPSSSSSSYHESSEIGGAMITTTNPYTVLRH 348

Query: 326 SIYRAFATAFMKEAAVLNFTLTNAVEPFGVCYAAESIAVTATGPTAPVLDLEMEKGKVVW 385
           SI+  F   F     V       AV PFG+CY  + I+        P +DL M+K  VVW
Sbjct: 349 SIFEVFTQVFANN--VPKQAQVKAVGPFGLCYDTKKIS-----GGVPSVDLIMDKSDVVW 408

Query: 386 KLGGRNSMVRIKKMGVDAWCLGFINGGEFPRTPIVIGGLQMEDHLLQFDLENYRFGFSSS 435
           ++ G N MV+ +  GV   CLGF++GG   R  I +G  Q+E++L+ FDL   R GF+++
Sbjct: 409 RISGENLMVQAQD-GVS--CLGFVDGGVHTRAGIALGTHQLEENLVVFDLARSRVGFNTN 444

BLAST of Tan0016285 vs. ExPASy Swiss-Prot
Match: Q8RVH5 (Basic 7S globulin 2 OS=Glycine max OX=3847 PE=1 SV=1)

HSP 1 Score: 172.2 bits (435), Expect = 1.3e-41
Identity = 138/451 (30.60%), Postives = 211/451 (46.78%), Query Frame = 0

Query: 6   FFFFTFSLISLP-----PLSLQTAFVAPIHKDHNSLLYSISVHLKTPLRPANLHLDLGGA 65
           F FF    + +P     P       V P+  D ++ L+  ++  +TPL    + +DL G 
Sbjct: 17  FLFFLSDSVPIPQHHTNPTKPINLLVLPVQNDASTGLHWANLQKRTPLMQVPVLVDLNGN 76

Query: 66  FSWIDCYNHYNSSSYQLVKCNSPLSNSLDHGICGSCIQAPTPICANDTIFSYLYPEKPSI 125
             W++C  HY+S +YQ   C+S   +  +   C SC  A  P C  +T    L    P  
Sbjct: 77  HLWVNCEQHYSSKTYQAPFCHSTQCSRANTHQCLSCPAASRPGCHKNT--CGLMSTNPIT 136

Query: 126 RDQFVDYDHPKLTDSENLITDVLALSTTDGLLRRFS---NIP---FACVKTDFL-RGLAK 185
           +           T    L  DVLA+  T G  ++      +P   F+C  +  L +GL +
Sbjct: 137 QQ----------TGLGELGQDVLAIHATQGSTQQLGPLVTVPQFLFSCAPSFLLQKGLPR 196

Query: 186 NVIGLAALGRANESIPSMISSKFNSPRIFAICLSGTRLGSGVAFFG-SKGPYRFSPNVDL 245
           N+ G+A LG A  S+P+ ++S F     F  CLS      G   FG +    +   N D+
Sbjct: 197 NIQGVAGLGHAPISLPNQLASHFGLQHQFTTCLSRYPTSKGALIFGDAPNNMQQFHNQDI 256

Query: 246 SKSLIYTPLLFNPHSASIYTHWLPSYEYYIGLTAIRINGEAVRFNTSLLAFEPVHGRGGA 305
              L +TPL   P             EY + +++IRIN  +V F  + ++   V   GG 
Sbjct: 257 FHDLAFTPLTVTPQG-----------EYNVRVSSIRINQHSV-FPPNKISSTIVGSSGGT 316

Query: 306 KISTSTTYALLQSSIYRAFATAFMKEAAVLNFTLTNAVEPFGVCYAAESIAVTATGPTAP 365
            ISTST + +LQ S+Y+AF   F ++  +       +V PFG+C+ +  I         P
Sbjct: 317 MISTSTPHMVLQQSLYQAFTQVFAQQ--LEKQAQVKSVAPFGLCFNSNKI------NAYP 376

Query: 366 VLDLEMEK-GKVVWKLGGRNSMVRIKKMGVDAWCLGFINGGEFPRTPIVIGGLQMEDHLL 425
            +DL M+K    VW++ G + MV+ +  GV   CLG +NGG  PR  + +G  Q+E+ L+
Sbjct: 377 SVDLVMDKPNGPVWRISGEDLMVQAQP-GVT--CLGVMNGGMQPRAEVTLGTRQLEEKLM 432

Query: 426 QFDLENYRFGFSSSAL-LQGTSCSQ-FNFTS 441
            FDL   R GFS+S+L   G  C   FNF +
Sbjct: 437 VFDLARSRVGFSTSSLHSHGVKCGDLFNFAN 432

BLAST of Tan0016285 vs. NCBI nr
Match: XP_022959602.1 (basic 7S globulin 2-like [Cucurbita moschata])

HSP 1 Score: 776.2 bits (2003), Expect = 1.6e-220
Identity = 381/444 (85.81%), Postives = 411/444 (92.57%), Query Frame = 0

Query: 6   FFFFTFSLISLPPLSLQTAFVAPIHKDHNSLLYSISVHLKTPLRPANLHLDLGGAFSWID 65
           F FFTFSL+S P LSLQTAF+APIHKDHNS LYSISVHLKTPLRPANLHLDLGGAFSWID
Sbjct: 7   FVFFTFSLLSFPFLSLQTAFIAPIHKDHNSGLYSISVHLKTPLRPANLHLDLGGAFSWID 66

Query: 66  CYNHYNSSSYQLVKCNSPLSNSLDHGICGSCIQAPTPICANDTIFSYLYPEKPSIRDQFV 125
           CYNHYNSSSY+LVKCNSPLS+S +HG+CGSCIQAPTPICANDTIFSY+YPEKPSIRD++V
Sbjct: 67  CYNHYNSSSYRLVKCNSPLSDSFNHGVCGSCIQAPTPICANDTIFSYVYPEKPSIRDEYV 126

Query: 126 DYDHPKLTDSENLITDVLALSTTD----GLLRRFSNIPFACVKTDFLRGLAKNVIGLAAL 185
           DY + KLTDSEN++TDVLALSTTD    G LRR SN+PFACVKT+FLRGLA+NVIGLAAL
Sbjct: 127 DYLNAKLTDSENVVTDVLALSTTDGSRSGSLRRISNMPFACVKTNFLRGLARNVIGLAAL 186

Query: 186 GRANESIPSMISSKFNSPRIFAICLSGTRLGSGVAFFGSKGPYRFSPNVDLSKSLIYTPL 245
           GRANESIP  IS+KFNSPRIFAICLSGTRLGSGVAF GSKGPY FSPNVDLSKSLIYTPL
Sbjct: 187 GRANESIPLTISAKFNSPRIFAICLSGTRLGSGVAFIGSKGPYAFSPNVDLSKSLIYTPL 246

Query: 246 LFNPHSASIYTHWLPSYEYYIGLTAIRINGEAVRFNTSLLAFEPVHGRGGAKISTSTTYA 305
           LFNP S SIYT+WLPSYEYYIGL+AIRIN EAVRFNTSLL FEPVHGRGGAKISTSTTYA
Sbjct: 247 LFNPQSGSIYTNWLPSYEYYIGLSAIRINNEAVRFNTSLLQFEPVHGRGGAKISTSTTYA 306

Query: 306 LLQSSIYRAFATAFMKEAAVLNFTLTNAVEPFGVCYAAESIAVTATGPTAPVLDLEMEKG 365
           LLQSSIYRAFA AFMKEAA+LNFTL NAVEPFGVC+   S+ +TA GP APV+ LEMEKG
Sbjct: 307 LLQSSIYRAFAMAFMKEAAMLNFTLANAVEPFGVCFEGSSVEMTAAGPRAPVVYLEMEKG 366

Query: 366 KVVWKLGGRNSMVRIKKMGVDAWCLGFINGGEFPRTPIVIGGLQMEDHLLQFDLENYRFG 425
           KVVWKLGGRNSMVRIKK+GVD WCLG++NGGEFPRTPIVIGGLQMEDHLLQFDLE YRFG
Sbjct: 367 KVVWKLGGRNSMVRIKKLGVDLWCLGYVNGGEFPRTPIVIGGLQMEDHLLQFDLEKYRFG 426

Query: 426 FSSSALLQGTSCSQFNFTSVESFL 446
           FSSSALLQGTSCS+FNF+S ++FL
Sbjct: 427 FSSSALLQGTSCSKFNFSSSDNFL 450

BLAST of Tan0016285 vs. NCBI nr
Match: XP_023004536.1 (basic 7S globulin 2-like [Cucurbita maxima])

HSP 1 Score: 764.6 bits (1973), Expect = 4.7e-217
Identity = 374/444 (84.23%), Postives = 407/444 (91.67%), Query Frame = 0

Query: 6   FFFFTFSLISLPPLSLQTAFVAPIHKDHNSLLYSISVHLKTPLRPANLHLDLGGAFSWID 65
           F FFTFSL+S P  SLQTAF+APIHKDHNSLLYSISVH+KTPLRPANLHLDLGGAFSWID
Sbjct: 7   FVFFTFSLLSFPFFSLQTAFIAPIHKDHNSLLYSISVHVKTPLRPANLHLDLGGAFSWID 66

Query: 66  CYNHYNSSSYQLVKCNSPLSNSLDHGICGSCIQAPTPICANDTIFSYLYPEKPSIRDQFV 125
           CYNHYNSSSY+LVKCNSPLS+S +HG+CGSCIQAPTP C NDTIF+Y+YPEKPSIRD++V
Sbjct: 67  CYNHYNSSSYRLVKCNSPLSDSFNHGVCGSCIQAPTPTCGNDTIFTYVYPEKPSIRDEYV 126

Query: 126 DYDHPKLTDSENLITDVLALSTTD----GLLRRFSNIPFACVKTDFLRGLAKNVIGLAAL 185
           DY + KLTDSEN++TDVLALSTTD    G LR+ S +PFACVKT+FLRGLA+NVIGLAAL
Sbjct: 127 DYLNAKLTDSENVVTDVLALSTTDGSRSGSLRQVSYMPFACVKTNFLRGLARNVIGLAAL 186

Query: 186 GRANESIPSMISSKFNSPRIFAICLSGTRLGSGVAFFGSKGPYRFSPNVDLSKSLIYTPL 245
           GRANESIP  IS+KFNSPRIFAICLSGTRLGSGVAFFGSKGPY FSPNVDLSKSLIYTPL
Sbjct: 187 GRANESIPLTISAKFNSPRIFAICLSGTRLGSGVAFFGSKGPYIFSPNVDLSKSLIYTPL 246

Query: 246 LFNPHSASIYTHWLPSYEYYIGLTAIRINGEAVRFNTSLLAFEPVHGRGGAKISTSTTYA 305
           LFNP S SIYT+WLPSYEYYIGL+AIRIN E VRFNTSLL FEPVHGRGGAKISTSTTYA
Sbjct: 247 LFNPQSGSIYTNWLPSYEYYIGLSAIRINNEVVRFNTSLLQFEPVHGRGGAKISTSTTYA 306

Query: 306 LLQSSIYRAFATAFMKEAAVLNFTLTNAVEPFGVCYAAESIAVTATGPTAPVLDLEMEKG 365
           LLQSSIYRAFA AFMKEAA LNFTL NAVEPFGVC+   S+ +TA GP APV+ LEMEKG
Sbjct: 307 LLQSSIYRAFAMAFMKEAARLNFTLANAVEPFGVCFEGSSVEMTAAGPRAPVVYLEMEKG 366

Query: 366 KVVWKLGGRNSMVRIKKMGVDAWCLGFINGGEFPRTPIVIGGLQMEDHLLQFDLENYRFG 425
           KVVWKLGGRNSMVRIKK+GVD WCLG++NGGEFPRTPIVIGGLQMEDHLLQFDLE YRFG
Sbjct: 367 KVVWKLGGRNSMVRIKKLGVDLWCLGYVNGGEFPRTPIVIGGLQMEDHLLQFDLEKYRFG 426

Query: 426 FSSSALLQGTSCSQFNFTSVESFL 446
           FSSSAL+QGTSCS+FNF+S ++FL
Sbjct: 427 FSSSALMQGTSCSKFNFSSSDNFL 450

BLAST of Tan0016285 vs. NCBI nr
Match: KAG7027263.1 (hypothetical protein SDJN02_11275, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 764.2 bits (1972), Expect = 6.1e-217
Identity = 376/444 (84.68%), Postives = 409/444 (92.12%), Query Frame = 0

Query: 6   FFFFTFSLISLPPLSLQTAFVAPIHKDHNSLLYSISVHLKTPLRPANLHLDLGGAFSWID 65
           F FFTFSL+S P LSLQTAF+APIHKDHNS LYSISVH+KTPLRPANLHLDLGGAFSWID
Sbjct: 7   FVFFTFSLLSFPFLSLQTAFIAPIHKDHNSGLYSISVHVKTPLRPANLHLDLGGAFSWID 66

Query: 66  CYNHYNSSSYQLVKCNSPLSNSLDHGICGSCIQAPTPICANDTIFSYLYPEKPSIRDQFV 125
           CYNHYNSSSY+LVKCNSPLS+S +H +C SCIQAPT ICAND IFS +YPEKPSIRD++V
Sbjct: 67  CYNHYNSSSYRLVKCNSPLSDSFNHRVCSSCIQAPTSICANDAIFSDVYPEKPSIRDEYV 126

Query: 126 DYDHPKLTDSENLITDVLALSTTD----GLLRRFSNIPFACVKTDFLRGLAKNVIGLAAL 185
           DY +PKLTDSEN++TDVLALSTTD    GLLRR SN+PFACVKT+FLRGLA+NVIGLAAL
Sbjct: 127 DYLNPKLTDSENVVTDVLALSTTDGSRPGLLRRISNMPFACVKTNFLRGLARNVIGLAAL 186

Query: 186 GRANESIPSMISSKFNSPRIFAICLSGTRLGSGVAFFGSKGPYRFSPNVDLSKSLIYTPL 245
           GRANESIP  IS+KFNSPRIFAICLSGTRLGSGVAF GSKGPY FSPNVDLSKSLIYTPL
Sbjct: 187 GRANESIPLTISAKFNSPRIFAICLSGTRLGSGVAFIGSKGPYVFSPNVDLSKSLIYTPL 246

Query: 246 LFNPHSASIYTHWLPSYEYYIGLTAIRINGEAVRFNTSLLAFEPVHGRGGAKISTSTTYA 305
           LFNP S SIYT+WLPSYEYYIGL+AIRIN EAVRFNTSLL FEPVHGRGGAKISTSTTYA
Sbjct: 247 LFNPQSGSIYTNWLPSYEYYIGLSAIRINNEAVRFNTSLLQFEPVHGRGGAKISTSTTYA 306

Query: 306 LLQSSIYRAFATAFMKEAAVLNFTLTNAVEPFGVCYAAESIAVTATGPTAPVLDLEMEKG 365
           LL+SSIYRAFATAFMKEAA+LNFTL NAVEPFGVC+   S+A+TA GP APV+ LEMEKG
Sbjct: 307 LLESSIYRAFATAFMKEAAMLNFTLANAVEPFGVCFEGSSVAMTAAGPRAPVVYLEMEKG 366

Query: 366 KVVWKLGGRNSMVRIKKMGVDAWCLGFINGGEFPRTPIVIGGLQMEDHLLQFDLENYRFG 425
           KVVWKLGGRNSMVRIKK+GVD WCLG++NGGEFPRTPIVIGGLQMEDHLLQFDLE +RFG
Sbjct: 367 KVVWKLGGRNSMVRIKKLGVDLWCLGYVNGGEFPRTPIVIGGLQMEDHLLQFDLEKHRFG 426

Query: 426 FSSSALLQGTSCSQFNFTSVESFL 446
           FSSSALLQGTSCS+FNF+S ++ L
Sbjct: 427 FSSSALLQGTSCSKFNFSSSDNLL 450

BLAST of Tan0016285 vs. NCBI nr
Match: XP_023514746.1 (basic 7S globulin 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 761.1 bits (1964), Expect = 5.2e-216
Identity = 374/444 (84.23%), Postives = 408/444 (91.89%), Query Frame = 0

Query: 6   FFFFTFSLISLPPLSLQTAFVAPIHKDHNSLLYSISVHLKTPLRPANLHLDLGGAFSWID 65
           F FFTFSL+  P LSLQTAF+APIHKDHNS LYSISVH+KTPLRPANLHLDLGGAFSWID
Sbjct: 7   FVFFTFSLLPFPFLSLQTAFIAPIHKDHNSGLYSISVHVKTPLRPANLHLDLGGAFSWID 66

Query: 66  CYNHYNSSSYQLVKCNSPLSNSLDHGICGSCIQAPTPICANDTIFSYLYPEKPSIRDQFV 125
           CYNHYNSSSY+LVKCNSPLS+S +H +C SCIQAPT ICAND IFS +YPEKPSIRD++V
Sbjct: 67  CYNHYNSSSYRLVKCNSPLSDSFNHRVCSSCIQAPTSICANDAIFSDVYPEKPSIRDEYV 126

Query: 126 DYDHPKLTDSENLITDVLALSTTD----GLLRRFSNIPFACVKTDFLRGLAKNVIGLAAL 185
           DY +PKLTDSEN++TDVLALSTTD    GLLRR SN+PFACVKT+FLRGLA+NVIGLAAL
Sbjct: 127 DYLNPKLTDSENVVTDVLALSTTDGSRSGLLRRISNMPFACVKTNFLRGLARNVIGLAAL 186

Query: 186 GRANESIPSMISSKFNSPRIFAICLSGTRLGSGVAFFGSKGPYRFSPNVDLSKSLIYTPL 245
           GRANESIP  IS+KFNSPRIFAICLSGTRLGSGVAF GSKGPY FSPNVDLSKSLIYTPL
Sbjct: 187 GRANESIPLTISAKFNSPRIFAICLSGTRLGSGVAFIGSKGPYVFSPNVDLSKSLIYTPL 246

Query: 246 LFNPHSASIYTHWLPSYEYYIGLTAIRINGEAVRFNTSLLAFEPVHGRGGAKISTSTTYA 305
           LFNP S SIYT+WLPSYEYYIGL+AIRIN EAVRFNTSLL FEPVHGRGGAKISTSTTYA
Sbjct: 247 LFNPQSGSIYTNWLPSYEYYIGLSAIRINNEAVRFNTSLLQFEPVHGRGGAKISTSTTYA 306

Query: 306 LLQSSIYRAFATAFMKEAAVLNFTLTNAVEPFGVCYAAESIAVTATGPTAPVLDLEMEKG 365
           LL+SSIYRAFATAFMKEAA+LNFTL NAVEPFGVC+   S+A+TA GP APV+ LEMEKG
Sbjct: 307 LLESSIYRAFATAFMKEAAMLNFTLANAVEPFGVCFEGSSVAMTAAGPRAPVVYLEMEKG 366

Query: 366 KVVWKLGGRNSMVRIKKMGVDAWCLGFINGGEFPRTPIVIGGLQMEDHLLQFDLENYRFG 425
           KVVWKLGGRNSMVRIKK+GVD WCLG++NGGEFPRTPIVIGGLQMEDHLLQFDLE +RFG
Sbjct: 367 KVVWKLGGRNSMVRIKKLGVDLWCLGYVNGGEFPRTPIVIGGLQMEDHLLQFDLEKHRFG 426

Query: 426 FSSSALLQGTSCSQFNFTSVESFL 446
           F+SSALLQGTSCS+FNF+S ++ L
Sbjct: 427 FTSSALLQGTSCSKFNFSSSDNLL 450

BLAST of Tan0016285 vs. NCBI nr
Match: KAG6593130.1 (putative aspartic proteinase GIP1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 756.9 bits (1953), Expect = 9.8e-215
Identity = 372/444 (83.78%), Postives = 407/444 (91.67%), Query Frame = 0

Query: 6   FFFFTFSLISLPPLSLQTAFVAPIHKDHNSLLYSISVHLKTPLRPANLHLDLGGAFSWID 65
           F FFTFSL+S P LSLQTAF+APIHKDHNS LYSISVH+KTPLRPANLHLDLGGAFSWID
Sbjct: 7   FVFFTFSLLSFPFLSLQTAFIAPIHKDHNSGLYSISVHVKTPLRPANLHLDLGGAFSWID 66

Query: 66  CYNHYNSSSYQLVKCNSPLSNSLDHGICGSCIQAPTPICANDTIFSYLYPEKPSIRDQFV 125
           CYNHYNSSSY+LVKCNSPLS+S +H +C SCIQAPT ICAND IFS +YPEKPSIRD++V
Sbjct: 67  CYNHYNSSSYRLVKCNSPLSDSFNHRVCSSCIQAPTSICANDAIFSDVYPEKPSIRDEYV 126

Query: 126 DYDHPKLTDSENLITDVLALSTTD----GLLRRFSNIPFACVKTDFLRGLAKNVIGLAAL 185
           DY +PKLTDSEN++TDVLALSTTD    G LRR SN+PFACVKT+FLRGLA+N+IGLAAL
Sbjct: 127 DYLNPKLTDSENVVTDVLALSTTDGSRSGSLRRISNMPFACVKTNFLRGLARNIIGLAAL 186

Query: 186 GRANESIPSMISSKFNSPRIFAICLSGTRLGSGVAFFGSKGPYRFSPNVDLSKSLIYTPL 245
           GRANESIP  IS++FNSPRIFAICLSGTRLGSGVAF GSKGPY FS NVDLSKSLIYTPL
Sbjct: 187 GRANESIPLTISAEFNSPRIFAICLSGTRLGSGVAFIGSKGPYVFSTNVDLSKSLIYTPL 246

Query: 246 LFNPHSASIYTHWLPSYEYYIGLTAIRINGEAVRFNTSLLAFEPVHGRGGAKISTSTTYA 305
           LFNP S SIYT+WLPSYEYYIGL+AIRIN EAVRFNTSLL FEPVHGRGGAKISTSTTYA
Sbjct: 247 LFNPQSGSIYTNWLPSYEYYIGLSAIRINNEAVRFNTSLLQFEPVHGRGGAKISTSTTYA 306

Query: 306 LLQSSIYRAFATAFMKEAAVLNFTLTNAVEPFGVCYAAESIAVTATGPTAPVLDLEMEKG 365
           LL+SSIYRAFATAFMKEAA+LNFTL NAVEPFGVC+   S+A+TA GP APV+ LEMEKG
Sbjct: 307 LLESSIYRAFATAFMKEAAMLNFTLANAVEPFGVCFEGSSVAMTAAGPRAPVVYLEMEKG 366

Query: 366 KVVWKLGGRNSMVRIKKMGVDAWCLGFINGGEFPRTPIVIGGLQMEDHLLQFDLENYRFG 425
           KVVWKLGGRNSMVRIKK+GVD WCLG++NGGEFPRTPIVIGGLQMEDHLLQFDLE +RFG
Sbjct: 367 KVVWKLGGRNSMVRIKKLGVDLWCLGYVNGGEFPRTPIVIGGLQMEDHLLQFDLEKHRFG 426

Query: 426 FSSSALLQGTSCSQFNFTSVESFL 446
           FSSSALLQGTSCS+FNF+S ++ L
Sbjct: 427 FSSSALLQGTSCSKFNFSSSDNLL 450

BLAST of Tan0016285 vs. ExPASy TrEMBL
Match: A0A6J1H501 (basic 7S globulin 2-like OS=Cucurbita moschata OX=3662 GN=LOC111460627 PE=4 SV=1)

HSP 1 Score: 776.2 bits (2003), Expect = 7.5e-221
Identity = 381/444 (85.81%), Postives = 411/444 (92.57%), Query Frame = 0

Query: 6   FFFFTFSLISLPPLSLQTAFVAPIHKDHNSLLYSISVHLKTPLRPANLHLDLGGAFSWID 65
           F FFTFSL+S P LSLQTAF+APIHKDHNS LYSISVHLKTPLRPANLHLDLGGAFSWID
Sbjct: 7   FVFFTFSLLSFPFLSLQTAFIAPIHKDHNSGLYSISVHLKTPLRPANLHLDLGGAFSWID 66

Query: 66  CYNHYNSSSYQLVKCNSPLSNSLDHGICGSCIQAPTPICANDTIFSYLYPEKPSIRDQFV 125
           CYNHYNSSSY+LVKCNSPLS+S +HG+CGSCIQAPTPICANDTIFSY+YPEKPSIRD++V
Sbjct: 67  CYNHYNSSSYRLVKCNSPLSDSFNHGVCGSCIQAPTPICANDTIFSYVYPEKPSIRDEYV 126

Query: 126 DYDHPKLTDSENLITDVLALSTTD----GLLRRFSNIPFACVKTDFLRGLAKNVIGLAAL 185
           DY + KLTDSEN++TDVLALSTTD    G LRR SN+PFACVKT+FLRGLA+NVIGLAAL
Sbjct: 127 DYLNAKLTDSENVVTDVLALSTTDGSRSGSLRRISNMPFACVKTNFLRGLARNVIGLAAL 186

Query: 186 GRANESIPSMISSKFNSPRIFAICLSGTRLGSGVAFFGSKGPYRFSPNVDLSKSLIYTPL 245
           GRANESIP  IS+KFNSPRIFAICLSGTRLGSGVAF GSKGPY FSPNVDLSKSLIYTPL
Sbjct: 187 GRANESIPLTISAKFNSPRIFAICLSGTRLGSGVAFIGSKGPYAFSPNVDLSKSLIYTPL 246

Query: 246 LFNPHSASIYTHWLPSYEYYIGLTAIRINGEAVRFNTSLLAFEPVHGRGGAKISTSTTYA 305
           LFNP S SIYT+WLPSYEYYIGL+AIRIN EAVRFNTSLL FEPVHGRGGAKISTSTTYA
Sbjct: 247 LFNPQSGSIYTNWLPSYEYYIGLSAIRINNEAVRFNTSLLQFEPVHGRGGAKISTSTTYA 306

Query: 306 LLQSSIYRAFATAFMKEAAVLNFTLTNAVEPFGVCYAAESIAVTATGPTAPVLDLEMEKG 365
           LLQSSIYRAFA AFMKEAA+LNFTL NAVEPFGVC+   S+ +TA GP APV+ LEMEKG
Sbjct: 307 LLQSSIYRAFAMAFMKEAAMLNFTLANAVEPFGVCFEGSSVEMTAAGPRAPVVYLEMEKG 366

Query: 366 KVVWKLGGRNSMVRIKKMGVDAWCLGFINGGEFPRTPIVIGGLQMEDHLLQFDLENYRFG 425
           KVVWKLGGRNSMVRIKK+GVD WCLG++NGGEFPRTPIVIGGLQMEDHLLQFDLE YRFG
Sbjct: 367 KVVWKLGGRNSMVRIKKLGVDLWCLGYVNGGEFPRTPIVIGGLQMEDHLLQFDLEKYRFG 426

Query: 426 FSSSALLQGTSCSQFNFTSVESFL 446
           FSSSALLQGTSCS+FNF+S ++FL
Sbjct: 427 FSSSALLQGTSCSKFNFSSSDNFL 450

BLAST of Tan0016285 vs. ExPASy TrEMBL
Match: A0A6J1KSD2 (basic 7S globulin 2-like OS=Cucurbita maxima OX=3661 GN=LOC111497811 PE=4 SV=1)

HSP 1 Score: 764.6 bits (1973), Expect = 2.3e-217
Identity = 374/444 (84.23%), Postives = 407/444 (91.67%), Query Frame = 0

Query: 6   FFFFTFSLISLPPLSLQTAFVAPIHKDHNSLLYSISVHLKTPLRPANLHLDLGGAFSWID 65
           F FFTFSL+S P  SLQTAF+APIHKDHNSLLYSISVH+KTPLRPANLHLDLGGAFSWID
Sbjct: 7   FVFFTFSLLSFPFFSLQTAFIAPIHKDHNSLLYSISVHVKTPLRPANLHLDLGGAFSWID 66

Query: 66  CYNHYNSSSYQLVKCNSPLSNSLDHGICGSCIQAPTPICANDTIFSYLYPEKPSIRDQFV 125
           CYNHYNSSSY+LVKCNSPLS+S +HG+CGSCIQAPTP C NDTIF+Y+YPEKPSIRD++V
Sbjct: 67  CYNHYNSSSYRLVKCNSPLSDSFNHGVCGSCIQAPTPTCGNDTIFTYVYPEKPSIRDEYV 126

Query: 126 DYDHPKLTDSENLITDVLALSTTD----GLLRRFSNIPFACVKTDFLRGLAKNVIGLAAL 185
           DY + KLTDSEN++TDVLALSTTD    G LR+ S +PFACVKT+FLRGLA+NVIGLAAL
Sbjct: 127 DYLNAKLTDSENVVTDVLALSTTDGSRSGSLRQVSYMPFACVKTNFLRGLARNVIGLAAL 186

Query: 186 GRANESIPSMISSKFNSPRIFAICLSGTRLGSGVAFFGSKGPYRFSPNVDLSKSLIYTPL 245
           GRANESIP  IS+KFNSPRIFAICLSGTRLGSGVAFFGSKGPY FSPNVDLSKSLIYTPL
Sbjct: 187 GRANESIPLTISAKFNSPRIFAICLSGTRLGSGVAFFGSKGPYIFSPNVDLSKSLIYTPL 246

Query: 246 LFNPHSASIYTHWLPSYEYYIGLTAIRINGEAVRFNTSLLAFEPVHGRGGAKISTSTTYA 305
           LFNP S SIYT+WLPSYEYYIGL+AIRIN E VRFNTSLL FEPVHGRGGAKISTSTTYA
Sbjct: 247 LFNPQSGSIYTNWLPSYEYYIGLSAIRINNEVVRFNTSLLQFEPVHGRGGAKISTSTTYA 306

Query: 306 LLQSSIYRAFATAFMKEAAVLNFTLTNAVEPFGVCYAAESIAVTATGPTAPVLDLEMEKG 365
           LLQSSIYRAFA AFMKEAA LNFTL NAVEPFGVC+   S+ +TA GP APV+ LEMEKG
Sbjct: 307 LLQSSIYRAFAMAFMKEAARLNFTLANAVEPFGVCFEGSSVEMTAAGPRAPVVYLEMEKG 366

Query: 366 KVVWKLGGRNSMVRIKKMGVDAWCLGFINGGEFPRTPIVIGGLQMEDHLLQFDLENYRFG 425
           KVVWKLGGRNSMVRIKK+GVD WCLG++NGGEFPRTPIVIGGLQMEDHLLQFDLE YRFG
Sbjct: 367 KVVWKLGGRNSMVRIKKLGVDLWCLGYVNGGEFPRTPIVIGGLQMEDHLLQFDLEKYRFG 426

Query: 426 FSSSALLQGTSCSQFNFTSVESFL 446
           FSSSAL+QGTSCS+FNF+S ++FL
Sbjct: 427 FSSSALMQGTSCSKFNFSSSDNFL 450

BLAST of Tan0016285 vs. ExPASy TrEMBL
Match: A0A5D3D4L4 (Basic 7S globulin 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold2032G00050 PE=4 SV=1)

HSP 1 Score: 710.7 bits (1833), Expect = 3.9e-201
Identity = 350/448 (78.12%), Postives = 390/448 (87.05%), Query Frame = 0

Query: 1   MVSRVFFFFTFSLISLPPLSLQTAFVAPIHKDHNSLLYSISVHLKTPLRPANLHLDLGGA 60
           M + + FFF F LIS P  SLQTA VAP+ KDH SLLYSIS+HLKTPLRPA L+LDLGG 
Sbjct: 1   MSTPLLFFFFFFLISFPSYSLQTASVAPLFKDHISLLYSISLHLKTPLRPATLYLDLGGP 60

Query: 61  FSWIDCYNHYNSSSYQLVKCNSPLSNSLDHGICGSCIQAPTPICANDTIFSYLYPEKPSI 120
           FSWIDCY +YNSSSY+L+ CN+PLSNS + GICGSC+QAP+P CANDTIF+Y YP+ PS+
Sbjct: 61  FSWIDCYQNYNSSSYKLLLCNTPLSNSFNQGICGSCVQAPSPTCANDTIFTYAYPQNPSL 120

Query: 121 RDQFVDYDHPKLTDSENLITDVLALSTTDGL----LRRFSNIPFACVKTDFLRGLAKNVI 180
           RDQFVDYD P+LTDSEN+ITDVLALSTTDG     LRR S IPFACVKT+FLRGLAKNVI
Sbjct: 121 RDQFVDYDRPELTDSENVITDVLALSTTDGSKSGPLRRISEIPFACVKTNFLRGLAKNVI 180

Query: 181 GLAALGRANESIPSMISSKFNSPRIFAICLSGTRLGSGVAFFGSKGPYRFSPNVDLSKSL 240
           GLAALGR+N SIPS+IS+KFNSP+ FAICLSG R G GVAFFGSKGPYRFSPNVDLSKSL
Sbjct: 181 GLAALGRSNLSIPSVISAKFNSPKFFAICLSGARSGPGVAFFGSKGPYRFSPNVDLSKSL 240

Query: 241 IYTPLLFNPHSASIYTHWLPSYEYYIGLTAIRINGEAVRFNTSLLAFEPVHGRGGAKIST 300
            YTPLLFNP S SI+T+WLPSYEYY+GL+AIRING+ V FNTSLL FEP+HG GGAKIST
Sbjct: 241 TYTPLLFNPASGSIHTYWLPSYEYYVGLSAIRINGKVVPFNTSLLPFEPIHGNGGAKIST 300

Query: 301 STTYALLQSSIYRAFATAFMKEAAVLNFTLTNAVEPFGVCYAAESIAVTATG-PTAPVLD 360
           ST Y LL+SSIYRAFA  FMKEAA LNF L NAV+PFGVCYAA+S+ VTA G   APV+D
Sbjct: 301 STNYGLLESSIYRAFARVFMKEAAALNFKLINAVKPFGVCYAAKSVGVTAEGHAKAPVVD 360

Query: 361 LEMEKGKVVWKLGGRNSMVRIKKMGVDAWCLGFINGGEFPRTPIVIGGLQMEDHLLQFDL 420
           L MEKGKVVWKLGGRN+MVRIKK GVDAWCLGFINGGEFPRTPIV+GGLQMEDHLLQFDL
Sbjct: 361 LVMEKGKVVWKLGGRNTMVRIKKKGVDAWCLGFINGGEFPRTPIVMGGLQMEDHLLQFDL 420

Query: 421 ENYRFGFSSSALLQGTSCSQFNFTSVES 444
           E +RFGFSSSAL +GTSCS+FNF S+ +
Sbjct: 421 EKFRFGFSSSALTEGTSCSKFNFNSINN 448

BLAST of Tan0016285 vs. ExPASy TrEMBL
Match: A0A0A0K506 (Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G390050 PE=4 SV=1)

HSP 1 Score: 708.4 bits (1827), Expect = 1.9e-200
Identity = 350/442 (79.19%), Postives = 387/442 (87.56%), Query Frame = 0

Query: 7   FFFTFSLISLPPLSLQTAFVAPIHKDHNSLLYSISVHLKTPLRPANLHLDLGGAFSWIDC 66
           FFF F LIS P  SLQTA +AP++K H SLLYSIS+HLKTPLRPA+L+LDLGGAFSWI C
Sbjct: 7   FFFFFFLISFPLYSLQTALIAPLYKHHTSLLYSISLHLKTPLRPASLYLDLGGAFSWIHC 66

Query: 67  YNHYNSSSYQLVKCNSPLSNSLDHGICGSCIQAPTPICANDTIFSYLYPEKPSIRDQFVD 126
           Y +YNSSSY+ V CN+PLSNS +  ICGSC+QAP+PICANDTIFSY YPE PS+RD FVD
Sbjct: 67  YQNYNSSSYKFVLCNTPLSNSFNQAICGSCVQAPSPICANDTIFSYAYPENPSLRDHFVD 126

Query: 127 YDHPKLTDSENLITDVLALSTTDGL----LRRFSNIPFACVKTDFLRGLAKNVIGLAALG 186
           YDHPKLTDSEN+ITDVLALSTT G     LRR    PFACVKT+FLR +AKNVIGLAALG
Sbjct: 127 YDHPKLTDSENVITDVLALSTTGGSTSAPLRRIPEFPFACVKTNFLREVAKNVIGLAALG 186

Query: 187 RANESIPSMISSKFNSPRIFAICLSGTRLGSGVAFFGSKGPYRFSPNVDLSKSLIYTPLL 246
           R+N SIPS+IS+KF+SP+ FAICLSG R G GVAFFGSKGPYRFSPNVDLSKSL YTPLL
Sbjct: 187 RSNLSIPSVISAKFSSPKYFAICLSGARSGPGVAFFGSKGPYRFSPNVDLSKSLTYTPLL 246

Query: 247 FNPHSASIYTHWLPSYEYYIGLTAIRINGEAVRFNTSLLAFEPVHGRGGAKISTSTTYAL 306
           FNP SASIYT+WLPSYEYY+GL+AIRING+ V FNTSLL+FEP+HGRGGAKISTST YAL
Sbjct: 247 FNPVSASIYTYWLPSYEYYVGLSAIRINGKVVPFNTSLLSFEPIHGRGGAKISTSTNYAL 306

Query: 307 LQSSIYRAFATAFMKEAAVLNFTLTNAVEPFGVCYAAESIAVTATGPT-APVLDLEMEKG 366
           L+SSIYRAFAT FMKEA VLNF L NAVEPFGVCY A+S+ VTA G   APV+DL MEK 
Sbjct: 307 LRSSIYRAFATVFMKEAVVLNFKLINAVEPFGVCYEAKSVGVTAEGQAKAPVVDLVMEKE 366

Query: 367 KVVWKLGGRNSMVRIKKMGVDAWCLGFINGGEFPRTPIVIGGLQMEDHLLQFDLENYRFG 426
           KVVWKLGGRN+MVRIKK GVDAWCLGFINGGEFPRTPIVIGGLQMEDHLLQFDLEN+RFG
Sbjct: 367 KVVWKLGGRNTMVRIKKKGVDAWCLGFINGGEFPRTPIVIGGLQMEDHLLQFDLENFRFG 426

Query: 427 FSSSALLQGTSCSQFNFTSVES 444
           FSSSAL +GTSCS+F+FTS  +
Sbjct: 427 FSSSALKEGTSCSKFDFTSANN 448

BLAST of Tan0016285 vs. ExPASy TrEMBL
Match: A0A6J1D7R8 (LOW QUALITY PROTEIN: basic 7S globulin 2 OS=Momordica charantia OX=3673 GN=LOC111018418 PE=4 SV=1)

HSP 1 Score: 578.6 bits (1490), Expect = 2.3e-161
Identity = 291/409 (71.15%), Postives = 330/409 (80.68%), Query Frame = 0

Query: 44  LKTPLRPANLHLDLGGAFSWIDCYNHYNSSSYQLVKCNSPLSNSLDHGICGSCIQAPTPI 103
           +KTPLRP  LHLDLGGAFSWIDCYNHYNSSSY+LV   S L NS+   I G C+ APTP 
Sbjct: 1   MKTPLRPTKLHLDLGGAFSWIDCYNHYNSSSYRLVDSESALCNSMHPNIAGPCVDAPTPF 60

Query: 104 CAND--TIFSYLYPEK-PSIRDQFVDYDHPKLTDSENLITDVLALSTTDGL----LRRFS 163
           C+N+  T+F Y+YPEK PSIRD  VDYDH KLTDSENLI DVLALSTTDG     LR+  
Sbjct: 61  CSNNGTTLFCYVYPEKPPSIRDS-VDYDHVKLTDSENLIVDVLALSTTDGSNPGPLRQIP 120

Query: 164 NIPFACVKTDFLRGLAKNVIGLAALGRANESIPSMISSKFNSPRIFAICLSGTRLGSGVA 223
           ++P +C           +VIGL ALG ANESIPSM+S+ F   + FA+CL G R G GVA
Sbjct: 121 HLPISC----------XDVIGLVALGXANESIPSMVSTTFGRSKYFAVCLPGARSGPGVA 180

Query: 224 FFGSKGPYRFSPNVDLSKSLIYTPLLFNPHSASIYTHWLPSYEYYIGLTAIRINGEAVRF 283
           FFGSKGPY+FSPNVDLSKSLIYTPLLFNP SASIYT+WLPSYEYYI L+AIRIN + V F
Sbjct: 181 FFGSKGPYKFSPNVDLSKSLIYTPLLFNPVSASIYTYWLPSYEYYIALSAIRINAKTVPF 240

Query: 284 NTSLLAFEPVHGRGGAKISTSTTYALLQSSIYRAFATAFMKEAAVLNFTLTNAVEPFGVC 343
           NTSLL FEP+HG GG KISTS TYALLQ+SIYRAFA AF KE+A LNFT T+ V+PFG+C
Sbjct: 241 NTSLLPFEPIHGGGGTKISTSATYALLQTSIYRAFAAAFAKESAALNFTATDPVKPFGLC 300

Query: 344 YAAESIAVTATGPTAPVLDLEMEKGKVVWKLGGRNSMVRIKKMGVDAWCLGFINGGEFPR 403
           YAAES+A+TA GP AP +DL ME GK  W+LGGRNSMVR+KK+GVDAWCLGFI+G E PR
Sbjct: 301 YAAESVAMTAAGPAAPAVDLVMEGGKSAWRLGGRNSMVRMKKVGVDAWCLGFIDGVENPR 360

Query: 404 TPIVIGGLQMEDHLLQFDLENYRFGFSSSALLQGTSCSQFNFTSVESFL 446
           TPIVIGGLQMED LLQFDLEN RFGF SS LLQGTSCS+FNFTS+++F+
Sbjct: 361 TPIVIGGLQMEDQLLQFDLENSRFGFGSSVLLQGTSCSKFNFTSLDNFI 398

BLAST of Tan0016285 vs. TAIR 10
Match: AT1G03220.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 297.7 bits (761), Expect = 1.5e-80
Identity = 179/450 (39.78%), Postives = 260/450 (57.78%), Query Frame = 0

Query: 1   MVSRVFFFFTFSLISLPPLSLQTAF-----VAPIHKDHNSLLYSISVHLKTPLRPANLHL 60
           ++  V   F FSL S    S QT F     + P+ KD ++L Y+  ++ +TPL PA++  
Sbjct: 6   IIFSVLLLFIFSLSS----SAQTPFRPKALLLPVTKDQSTLQYTTVINQRTPLVPASVVF 65

Query: 61  DLGGAFSWIDCYNHYNSSSYQLVKCNSPLSNSLDHGICGSCIQAPTPICANDTIFSYLYP 120
           DLGG   W+DC   Y SS+YQ  +CNS + +      CG+C   P P C+N+T       
Sbjct: 66  DLGGRELWVDCDKGYVSSTYQSPRCNSAVCSRAGSTSCGTCFSPPRPGCSNNTC------ 125

Query: 121 EKPSIRDQFVDYDHPKLTDSENLITDVLALSTTD----GLLRRFSNIPFACVKTDFLRGL 180
               I D  V         S     DV+++ +T+    G + +  N+ F C  T  L+GL
Sbjct: 126 --GGIPDNTV----TGTATSGEFALDVVSIQSTNGSNPGRVVKIPNLIFDCGATFLLKGL 185

Query: 181 AKNVIGLAALGRANESIPSMISSKFNSPRIFAICLSGTRLGSGVAFFGSKGPYRFSPNVD 240
           AK  +G+A +GR N  +PS  ++ F+  R FA+CL+    G GVAFFG+ GPY F P + 
Sbjct: 186 AKGTVGMAGMGRHNIGLPSQFAAAFSFHRKFAVCLTS---GKGVAFFGN-GPYVFLPGIQ 245

Query: 241 LSKSLIYTPLLFNPHS-ASIYTHWLPSYEYYIGLTAIRINGEAVRFNTSLLAFEPVHGRG 300
           +S SL  TPLL NP S AS ++    S EY+IG+TAI+I  + V  N +LL      G G
Sbjct: 246 IS-SLQTTPLLINPVSTASAFSQGEKSSEYFIGVTAIQIVEKTVPINPTLLKINASTGIG 305

Query: 301 GAKISTSTTYALLQSSIYRAFATAFMKEAAVLNFTLTNAVEPFGVCYAAESIAVTATGPT 360
           G KIS+   Y +L+SSIY AF + F+K+AA  +     +V+PFG C++ +++ VT  G  
Sbjct: 306 GTKISSVNPYTVLESSIYNAFTSEFVKQAAARSIKRVASVKPFGACFSTKNVGVTRLGYA 365

Query: 361 APVLDLEMEKGKVVWKLGGRNSMVRIKKMGVDAWCLGFINGGEFPRTPIVIGGLQMEDHL 420
            P ++L +    VVW++ G NSMV +     D  CLGF++GG   RT +VIGG Q+ED+L
Sbjct: 366 VPEIELVLHSKDVVWRIFGANSMVSVSD---DVICLGFVDGGVNARTSVVIGGFQLEDNL 425

Query: 421 LQFDLENYRFGFSSSALLQGTSCSQFNFTS 441
           ++FDL + +FGFSS+ L + T+C+ FNFTS
Sbjct: 426 IEFDLASNKFGFSSTLLGRQTNCANFNFTS 431

BLAST of Tan0016285 vs. TAIR 10
Match: AT1G03230.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 282.0 bits (720), Expect = 8.6e-76
Identity = 168/452 (37.17%), Postives = 255/452 (56.42%), Query Frame = 0

Query: 1   MVSRVFFFFTFSLISLPPLSLQT-------AFVAPIHKDHNSLLYSISVHLKTPLRPANL 60
           M S     F+  L+S+  LS          A + P+ KD ++L Y+  ++ +TPL PA++
Sbjct: 1   MASSRIIIFSVLLLSIFSLSSSAQPSFRPKALLLPVTKDPSTLQYTTVINQRTPLVPASV 60

Query: 61  HLDLGGAFSWIDCYNHYNSSSYQLVKCNSPLSNSLDHGICGSCIQAPTPICANDTIFSYL 120
             DLGG   W+DC   Y S++Y+  +CNS + +      CG+C   P P C+N+T     
Sbjct: 61  VFDLGGREFWVDCDQGYVSTTYRSPRCNSAVCSRAGSIACGTCFSPPRPGCSNNTC---- 120

Query: 121 YPEKPSIRDQFVDYDHPKLTDSENLITDVLALSTTDGL-LRRFSNIP---FACVKTDFLR 180
                     F D        S     DV+++ +T+G    RF  IP   F+C  T  L+
Sbjct: 121 --------GAFPDNSITGWATSGEFALDVVSIQSTNGSNPGRFVKIPNLIFSCGSTSLLK 180

Query: 181 GLAKNVIGLAALGRANESIPSMISSKFNSPRIFAICLSGTRLGSGVAFFGSKGPYRFSPN 240
           GLAK  +G+A +GR N  +P   ++ F+  R FA+CL+    G GVAFFG+ GPY F P 
Sbjct: 181 GLAKGAVGMAGMGRHNIGLPLQFAAAFSFNRKFAVCLTS---GRGVAFFGN-GPYVFLPG 240

Query: 241 VDLSKSLIYTPLLFNPHSASI-YTHWLPSYEYYIGLTAIRINGEAVRFNTSLLAFEPVHG 300
           + +S+ L  TPLL NP +    ++    S EY+IG+TAI+I  + +  + +LL      G
Sbjct: 241 IQISR-LQKTPLLINPGTTVFEFSKGEKSPEYFIGVTAIKIVEKTLPIDPTLLKINASTG 300

Query: 301 RGGAKISTSTTYALLQSSIYRAFATAFMKEAAVLNFTLTNAVEPFGVCYAAESIAVTATG 360
            GG KIS+   Y +L+SSIY+AF + F+++AA  +     +V+PFG C++ +++ VT  G
Sbjct: 301 IGGTKISSVNPYTVLESSIYKAFTSEFIRQAAARSIKRVASVKPFGACFSTKNVGVTRLG 360

Query: 361 PTAPVLDLEMEKGKVVWKLGGRNSMVRIKKMGVDAWCLGFINGGEFPRTPIVIGGLQMED 420
              P + L +    VVW++ G NSMV +     D  CLGF++GG  P   +VIGG Q+ED
Sbjct: 361 YAVPEIQLVLHSKDVVWRIFGANSMVSVSD---DVICLGFVDGGVNPGASVVIGGFQLED 420

Query: 421 HLLQFDLENYRFGFSSSALLQGTSCSQFNFTS 441
           +L++FDL + +FGFSS+ L + T+C+ FNFTS
Sbjct: 421 NLIEFDLASNKFGFSSTLLGRQTNCANFNFTS 432

BLAST of Tan0016285 vs. TAIR 10
Match: AT5G19110.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 163.3 bits (412), Expect = 4.4e-40
Identity = 128/434 (29.49%), Postives = 201/434 (46.31%), Query Frame = 0

Query: 12  SLISLPPLSLQTAFVAPIHKDHNSLLYSISVHLKTPLR-PANLHLDLGGAFSWIDCYNHY 71
           S+ +   L   + ++ PI K   + L+  + ++ +  + P NL LDLG   +W+DC    
Sbjct: 13  SIFAAIALKSNSQYLLPITKHEPTNLFYTTFNVGSAAKSPVNLLLDLGTNLTWLDCRKLK 72

Query: 72  NSSSYQLVKCNSPLSNSLDHGICG--SCI-QAPTPICANDTIFSYLYPEKPSIRDQFVDY 131
           + SS +LV C S    S+    C   SC+ + P P+  N  +                  
Sbjct: 73  SLSSLRLVTCQSSTCKSIPGNGCAGKSCLYKQPNPLGQNPVV------------------ 132

Query: 132 DHPKLTDSENLITDVLALSTTDG--LLRRFS--NIPFACVKTDFLRGLAKNVIGLAALGR 191
                  +  ++ D  +L TTDG   L + S  +  F+C     L+GL   V G+ AL  
Sbjct: 133 -------TGRVVQDRASLYTTDGGKFLSQVSVRHFTFSCAGEKALQGLPPPVDGVLALSP 192

Query: 192 ANESIPSMISSKFNSPRIFAICLSGTRLGSGVAFFGSKGPYRFSPNVDLSKSLIYTPLLF 251
            + S    ++S FN    F++CL      SG   F   G + F P  + S + I  P   
Sbjct: 193 GSSSFTKQVTSAFNVIPKFSLCLP----SSGTGHFYIAGIHYFIPPFNSSDNPI--PRTL 252

Query: 252 NPHSASIYTHWLPSYEYYIGLTAIRINGEAVRFNTSLLAFEPVHGRGGAKISTSTTYALL 311
            P   +       S +Y I + +I + G A++ N  LL        GGAK+ST   Y +L
Sbjct: 253 TPIKGT------DSGDYLITVKSIYVGGTALKLNPDLLT-------GGAKLSTVVHYTVL 312

Query: 312 QSSIYRAFATAFMKEAAVLNFTLTNAVEPFGVCYAAESIAVTAT-GPTAPVLDLEM--EK 371
           Q+ IY A A +F  +A  +      +V PF  C+ + +     T GP  PV+++ +    
Sbjct: 313 QTDIYNALAQSFTLKAKAMGIAKVPSVAPFKHCFDSRTAGKNLTAGPNVPVIEIGLPGRI 372

Query: 372 GKVVWKLGGRNSMVRIKKMGVDAWCLGFINGGEFPRTPIVIGGLQMEDHLLQFDLENYRF 431
           G+V W   G N++V++K+      CL FI+GG+ P+  +VIG  Q++DH+L+FD      
Sbjct: 373 GEVKWGFYGANTVVKVKE---TVMCLAFIDGGKTPKDLMVIGTHQLQDHMLEFDFSGTVL 399

Query: 432 GFSSSALLQGTSCS 435
            FS S LL  TSCS
Sbjct: 433 AFSESLLLHNTSCS 399

BLAST of Tan0016285 vs. TAIR 10
Match: AT5G19100.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 150.6 bits (379), Expect = 3.0e-36
Identity = 140/441 (31.75%), Postives = 207/441 (46.94%), Query Frame = 0

Query: 1   MVSRVFFFFTFSLISLPPLSLQTAFVAPIHKDHNSLLYSISVHLKTPLRPANLHLDLGGA 60
           ++S VF +   +  SL       +F+ PI+KD    +Y+I + + +        LDL GA
Sbjct: 9   LLSLVFLYLANTSHSLRKFQ---SFLHPIYKDTAKNIYTIPLSIGS-TSSEKFVLDLNGA 68

Query: 61  FSWI-DCYNHYNSSSYQLVKCNSPLSNSLDHGICGSCIQA-PTPICANDTIFSYLYPEKP 120
              + +C     S++Y  ++C S             C  A P   C N+ I         
Sbjct: 69  APLLQNCPTAAKSTTYHPIRCGST-----------RCKYANPNFPCPNNVI--------A 128

Query: 121 SIRDQFVDYDHPKL-TDSENLITDVLALSTTDGLLRRFSNIPFACVKTDFLRGLAKNVIG 180
             R   +  D+ +L  D+  L+     + T D  +   S++   C  TD    L +  IG
Sbjct: 129 KKRTVCLSSDNSRLFRDTVPLLYTFNGVYTRDSEMS--SSLTLTC--TDGAPALKQRTIG 188

Query: 181 LAALGRANESIPSMISSKFNSPRIFAICLSGTRLG---SGVAFFGSKGPYRFSP-NVDLS 240
           LA     + SIPS + S +  P   A+CL  T      +G  + G KG Y + P + D+S
Sbjct: 189 LA---NTHLSIPSQLISMYQLPHKIALCLPSTERSQSHNGDLWIG-KGEYYYLPYDKDVS 248

Query: 241 KSLIYTPLLFNPHSASIYTHWLPSYEYYIGLTAIRINGEAVRFNTSLLAFEPVHGRGGAK 300
           K    TPL+ N  S           EY I + +I+I  + V          P+   G  K
Sbjct: 249 KIFASTPLIGNGKSG----------EYLIDVKSIQIGAKTV----------PI-PYGATK 308

Query: 301 ISTSTTYALLQSSIYRAFATAFMKEAAVLNFTLTNAVEPFGVCYAAESIAVTATGPTAPV 360
           IST   Y + Q+S+Y+A  TAF +    +      AV+PFG C+ +        G   PV
Sbjct: 309 ISTLAPYTVFQTSLYKALLTAFTEN---IKIAKAPAVKPFGACFYSNG------GRGVPV 368

Query: 361 LDLEMEKGKVVWKLGGRNSMVRIKKMGVDAWCLGFINGGEFPRTPIVIGGLQMEDHLLQF 420
           +DL +  G   W++ G NS+V++ K  V   CLGF++GG  P+ PIVIGG QMED+L++F
Sbjct: 369 IDLVL-SGGAKWRIYGSNSLVKVNKNVV---CLGFVDGGVKPKYPIVIGGFQMEDNLVEF 384

Query: 421 DLENYRFGFSSSALLQGTSCS 435
           DLE  +F FSSS LL  TSCS
Sbjct: 429 DLEASKFSFSSSLLLHNTSCS 384

BLAST of Tan0016285 vs. TAIR 10
Match: AT5G19120.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 149.4 bits (376), Expect = 6.6e-36
Identity = 132/434 (30.41%), Postives = 192/434 (44.24%), Query Frame = 0

Query: 7   FFFTF---SLISLPPLS-LQTAFVAPIHKDHNSLLYSISVHLKTPLRPANLHLDLGGAFS 66
           FFF+F    +IS   +S      V P+ KD  +  Y   + L     P  L +DL G+  
Sbjct: 10  FFFSFLSALIISKSQISDSVNGVVFPVVKDLPTGQYLAQIRLGDSPDPVKLVVDLAGSIL 69

Query: 67  WIDCYNHYNSSSYQLVKCNSPLSNSLDHGICGSCIQAPTPICANDTIFSYLYPEKPSIRD 126
           W DC + + SSS  L+            G    C++A      N+ + S     K    D
Sbjct: 70  WFDCSSRHVSSSRNLIS-----------GSSSGCLKAKV---GNERVSSSSSSRKDQNAD 129

Query: 127 --QFVDYDHPKLTDSENLITDVLALS--TTDGLLRRFSNIPFACVKTDFLRGLAKNVIGL 186
               V  D   +T    L +DV+++   T+ G +    ++ FAC     LRGLA    G+
Sbjct: 130 CELLVKNDAFGITARGELFSDVMSVGSVTSPGTV----DLLFACTPPWLLRGLASGAQGV 189

Query: 187 AALGRANESIPSMISSKFNSPRIFAICLSGTRLGSGVAFFGSKGPYRFSPNVDLSKSLIY 246
             LGRA  S+PS ++++ N  R   + LS     +GV    S         V  S+SL+Y
Sbjct: 190 MGLGRAQISLPSQLAAETNERRRLTVYLSPL---NGVV---STSSVEEVFGVAASRSLVY 249

Query: 247 TPLLFNPHSASIYTHWLPSYEYYIGLTAIRINGEAVRFNTSLLAFEPVHGRGGAKISTST 306
           TPLL              S  Y I + +IR+NGE +           V G    ++ST  
Sbjct: 250 TPLLTG-----------SSGNYVINVKSIRVNGEKL----------SVEGPLAVELSTVV 309

Query: 307 TYALLQSSIYRAFATAFMKEAAVLNFTLTNAVEPFGVCYAAESIAVTATGPTAPVLDLEM 366
            Y +L+SSIY+ FA A+ K A     T    V PFG+C+ ++           P +DL +
Sbjct: 310 PYTILESSIYKVFAEAYAKAAG--EATSVPPVAPFGLCFTSD--------VDFPAVDLAL 369

Query: 367 EKGKVVWKLGGRNSMVRIKKMGVDAWCLGFINGGEFPRTPIVIGGLQMEDHLLQFDLENY 426
           +   V W++ G+N MV +   G    C G ++GG     PIV+GGLQ+E  +L FDL N 
Sbjct: 370 QSEMVRWRIHGKNLMVDV---GGGVRCSGIVDGGSSRVNPIVMGGLQLEGFILDFDLGNS 385

Query: 427 RFGFSSSALLQGTS 433
             GF        TS
Sbjct: 430 MMGFGQRTRSDSTS 385

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
I1JNS63.2e-9143.91Probable aspartic proteinase GIP1 OS=Glycine max OX=3847 GN=GIP1 PE=1 SV=2[more]
P0DO213.4e-7740.90Probable aspartic proteinase GIP2 OS=Nicotiana benthamiana OX=4100 GN=GIP2 PE=1 ... [more]
P829525.6e-5634.00Gamma conglutin 1 OS=Prunus dulcis OX=3755 GN=Cgamma1 PE=1 SV=2[more]
Q9FSH93.5e-4231.86Gamma conglutin 1 OS=Lupinus albus OX=3870 GN=Cgamma PE=1 SV=1[more]
Q8RVH51.3e-4130.60Basic 7S globulin 2 OS=Glycine max OX=3847 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_022959602.11.6e-22085.81basic 7S globulin 2-like [Cucurbita moschata][more]
XP_023004536.14.7e-21784.23basic 7S globulin 2-like [Cucurbita maxima][more]
KAG7027263.16.1e-21784.68hypothetical protein SDJN02_11275, partial [Cucurbita argyrosperma subsp. argyro... [more]
XP_023514746.15.2e-21684.23basic 7S globulin 2-like [Cucurbita pepo subsp. pepo][more]
KAG6593130.19.8e-21583.78putative aspartic proteinase GIP1, partial [Cucurbita argyrosperma subsp. sorori... [more]
Match NameE-valueIdentityDescription
A0A6J1H5017.5e-22185.81basic 7S globulin 2-like OS=Cucurbita moschata OX=3662 GN=LOC111460627 PE=4 SV=1[more]
A0A6J1KSD22.3e-21784.23basic 7S globulin 2-like OS=Cucurbita maxima OX=3661 GN=LOC111497811 PE=4 SV=1[more]
A0A5D3D4L43.9e-20178.13Basic 7S globulin 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold2032... [more]
A0A0A0K5061.9e-20079.19Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G39005... [more]
A0A6J1D7R82.3e-16171.15LOW QUALITY PROTEIN: basic 7S globulin 2 OS=Momordica charantia OX=3673 GN=LOC11... [more]
Match NameE-valueIdentityDescription
AT1G03220.11.5e-8039.78Eukaryotic aspartyl protease family protein [more]
AT1G03230.18.6e-7637.17Eukaryotic aspartyl protease family protein [more]
AT5G19110.14.4e-4029.49Eukaryotic aspartyl protease family protein [more]
AT5G19100.13.0e-3631.75Eukaryotic aspartyl protease family protein [more]
AT5G19120.16.6e-3630.41Eukaryotic aspartyl protease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR032799Xylanase inhibitor, C-terminalPFAMPF14541TAXi_Ccoord: 259..423
e-value: 4.8E-46
score: 156.5
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 18..220
e-value: 1.4E-37
score: 131.7
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 222..440
e-value: 7.7E-60
score: 203.8
IPR021109Aspartic peptidase domain superfamilySUPERFAMILY50630Acid proteasescoord: 30..432
IPR032861Xylanase inhibitor, N-terminalPFAMPF14543TAXi_Ncoord: 38..220
e-value: 9.1E-26
score: 91.2
IPR001461Aspartic peptidase A1 familyPANTHERPTHR47965ASPARTYL PROTEASE-RELATEDcoord: 7..438
NoneNo IPR availablePANTHERPTHR47965:SF6OS05G0403000 PROTEINcoord: 7..438
IPR033121Peptidase family A1 domainPROSITEPS51767PEPTIDASE_A1coord: 38..423
score: 19.500565

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0016285.1Tan0016285.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0004190 aspartic-type endopeptidase activity