Tan0015157 (gene) Snake gourd v1

Overview


Name	Tan0015157
Type	gene
Organism	Trichosanthes anguina (Snake gourd v1)
Description	thermospermine synthase ACAULIS5-like
Location	LG08: 460468 .. 462911 (+)
RNA-Seq Expression	Tan0015157
Synteny	Tan0015157

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTTTCATCCTTCTGCTCTGCCCTTTCTGTTCTCTTTATCCCCAACTCCACTGCTCTCCCTCTTGACCATCTCCACCAAGTCTCTGAATGGTACGAGGAACAGCTTGAGGATGATCTCAAATGGTCCTTTGCAGTCAACAGGTCCATCCTATTTTCTTCATCTTTCTCTTGCTCATAACATGCTTGTAAAATGTCCTCCCCTTTTGTTGTTCGTGTAATTCATGTTGTATTCGAATTCTCTATAGGCCTTTCATTGTAGAGTGCAGGAAACGAATTTGATAGCATGTTTTCAGCTAAACTGATCCTGACATTCACTTCCTTTAGGATACTTCATGCCACAACAAGCGAGTTCCAAGAAATAGTTCTCTTGGATACCAAGCGATTTGGGAAGGTTTGCTTTTATCATACATCAGTTCCCTTTGGCCTTTCCAATTATACTTGATCAGTACAGGGCTGAAAATGAGTTGACATTCATTGCAGGCTCTATTGCTTGATGGGAAGCTGCAAAGTGCTGAAAAAGATGAATTCATTTACCATGAGTCCTTGGTTCATCCTGCTTTGTTACTGCATCATAAGTATGCTGTGTTCATATTTGGTTTAGTTCTTTAACTATCCTTTTTGTTGGTCTGCAATCCAAGCTGATTTTGTTCTTATCTTCCCTTTTGATTTTAGCCCCAAAACAGTTTTTATAATGGGAGGTGGAGAAGGGTGCACAGCCAGAGAGACATTAAAGCACAAAAATATTGAGAAAGTTGTCATGTGCGATATTGATAGGGTATGGTTCTCAAAATCCTAATGATGCACTAATTTGTAAATTCATGTGGCAAAGGATCGATGAAGTTGAGTTGTATGACTAAAAAAATTAATTGGTAGAGACATATTGGTGCAAGAAAAGAAGAAGAACAATGAGAAATGGAGTGACAAATTTTCTTGATGTGATAAAAAGCAATGCTAGTTTCATATGAAGGACATGTTTGAGAGTGATTTTGGAATACTTGAAGTTTTTGATTTATACTGTACTGATATCTTTAGAGGGCAACACATTGTAGGAGGTTGTCAATTTCTGCCGTGAACATCTAACAGAAAACCAAGATGCATTTCAGGACGAGAAGCTTCATATTATTTTTGACGATGCAAAGTGAGTAGCTTTTCCCTTCGGTTCATTCCATCTGCCATTTGCATTTAGTTATGCAATTTGTGATGTTAAGCTTGTTAAAACTACGTCTCGACTTTTTCTTGAATCAATGCATATTTCCAAAGGATTCGAGTTTTGTAAGTTTAGATTTGAAAGAACTAACCCGATGCAAACATATGTTGAAAAATGGAAAAGGGCTGGATTGGAGGGACAACCAGAGAAGTTTGATGTAATCATTGGGGATTTATCCGATCCACATGAAGGAGGACCATGTAATCATCTGTATACTAAATCCTTTTATGAGGAAGTGATTAAGCCTAAGCTCAACGACAATGGCATCTTTGTTACTCAGGTACATTATATATACACATACACATATTGAAAGCCCTGCGTTATTATTCCTGCTTACTACTGTTACTAAGATTTCATTTACTGTTACTACAATGCAGGCTGGCCCTGCTGGTATTCTCTCCCACAAGGTCTTCTCATCAATATACAACACCGTAAAGCACGTCTTCCGATGTAATAAGATATTATAATTCGCCTGAATTTTCCTTTACAAAAAGTTGGTCAAAATTCTACCCGTTAGATGTGGAAAAACCATTGATGTAGATATAATGCCTTAATGAATTGAGCTATCCTCACTGGCTTAGATTGGACCCAAGTCTAAAAACAAATTCATGTAGATCTAAATTCAAAGCCGGATATGTCCTATCAATATAAAGATAGGAGAGCTCCGTTTGGCGCCTAACATTCGTTTGTTATATCTGCACAGATGTGATTGCATACACAGCTCATGTTCCATCCTATGCAGATTCATGTGGATGGGTTTTGGTAAGCAACTAGAAAAAATTCCTTGTATGTCTTATTGCTTGAAAGGAAAAATAGAAAGAGTTGAAACATTATAAATCTGTAACGACAGGCTTGTGATCATCCTCTTAAACTGGACATTGAGGACCTCAACAACAAGATACGTGAGAGAGTCCGAGGCGAGCTACACTACTTGGATGGTGCATTCATCGTATCGTCGACTGTCATAAACAAAACCATTCGTACGTTGTAAGTATAAGATAGTTCTTCATCGTTGAATATAATCATGTATCTTCTTGAGTGACCATTGATTTTTGAATCATGTGTAAAGGGAGAATGTGTATGTGTTTGTGGTGGTGCAGGATGATGAATGAGACTCATTTGTTCACTGAGGAAGATGCAAGGTTTGCACATGGCCAAGGGCTGGCTGGCAATGCCTAGAAGCTCCTTGGCCTTGTTGGCCAACCTCAACCTCAAACCAATATATTAGAACTCAG

mRNA sequence

ATGTTTTCATCCTTCTGCTCTGCCCTTTCTGTTCTCTTTATCCCCAACTCCACTGCTCTCCCTCTTGACCATCTCCACCAAGTCTCTGAATGGTACGAGGAACAGCTTGAGGATGATCTCAAATGGTCCTTTGCAGTCAACAGGATACTTCATGCCACAACAAGCGAGTTCCAAGAAATAGTTCTCTTGGATACCAAGCGATTTGGGAAGGCTCTATTGCTTGATGGGAAGCTGCAAAGTGCTGAAAAAGATGAATTCATTTACCATGAGTCCTTGGTTCATCCTGCTTTGTTACTGCATCATAACCCCAAAACAGTTTTTATAATGGGAGGTGGAGAAGGGTGCACAGCCAGAGAGACATTAAAGCACAAAAATATTGAGAAAGTTGTCATGTGCGATATTGATAGGGAGGTTGTCAATTTCTGCCGTGAACATCTAACAGAAAACCAAGATGCATTTCAGGACGAGAAGCTTCATATTATTTTTGACGATGCAAAGGCTGGATTGGAGGGACAACCAGAGAAGTTTGATGTAATCATTGGGGATTTATCCGATCCACATGAAGGAGGACCATGTAATCATCTGTATACTAAATCCTTTTATGAGGAAGTGATTAAGCCTAAGCTCAACGACAATGGCATCTTTGTTACTCAGGCTGGCCCTGCTGGTATTCTCTCCCACAAGGTCTTCTCATCAATATACAACACCGTAAAGCACGTCTTCCGATATGTGATTGCATACACAGCTCATGTTCCATCCTATGCAGATTCATGTGGATGGGTTTTGGCTTGTGATCATCCTCTTAAACTGGACATTGAGGACCTCAACAACAAGATACGTGAGAGAGTCCGAGGCGAGCTACACTACTTGGATGGTGCATTCATCGTATCGTCGACTGTCATAAACAAAACCATTCGTACGTTGATGATGAATGAGACTCATTTGTTCACTGAGGAAGATGCAAGGTTTGCACATGGCCAAGGGCTGGCTGGCAATGCCTAGAAGCTCCTTGGCCTTGTTGGCCAACCTCAACCTCAAACCAATATATTAGAACTCAG

Coding sequence (CDS)

ATGTTTTCATCCTTCTGCTCTGCCCTTTCTGTTCTCTTTATCCCCAACTCCACTGCTCTCCCTCTTGACCATCTCCACCAAGTCTCTGAATGGTACGAGGAACAGCTTGAGGATGATCTCAAATGGTCCTTTGCAGTCAACAGGATACTTCATGCCACAACAAGCGAGTTCCAAGAAATAGTTCTCTTGGATACCAAGCGATTTGGGAAGGCTCTATTGCTTGATGGGAAGCTGCAAAGTGCTGAAAAAGATGAATTCATTTACCATGAGTCCTTGGTTCATCCTGCTTTGTTACTGCATCATAACCCCAAAACAGTTTTTATAATGGGAGGTGGAGAAGGGTGCACAGCCAGAGAGACATTAAAGCACAAAAATATTGAGAAAGTTGTCATGTGCGATATTGATAGGGAGGTTGTCAATTTCTGCCGTGAACATCTAACAGAAAACCAAGATGCATTTCAGGACGAGAAGCTTCATATTATTTTTGACGATGCAAAGGCTGGATTGGAGGGACAACCAGAGAAGTTTGATGTAATCATTGGGGATTTATCCGATCCACATGAAGGAGGACCATGTAATCATCTGTATACTAAATCCTTTTATGAGGAAGTGATTAAGCCTAAGCTCAACGACAATGGCATCTTTGTTACTCAGGCTGGCCCTGCTGGTATTCTCTCCCACAAGGTCTTCTCATCAATATACAACACCGTAAAGCACGTCTTCCGATATGTGATTGCATACACAGCTCATGTTCCATCCTATGCAGATTCATGTGGATGGGTTTTGGCTTGTGATCATCCTCTTAAACTGGACATTGAGGACCTCAACAACAAGATACGTGAGAGAGTCCGAGGCGAGCTACACTACTTGGATGGTGCATTCATCGTATCGTCGACTGTCATAAACAAAACCATTCGTACGTTGATGATGAATGAGACTCATTTGTTCACTGAGGAAGATGCAAGGTTTGCACATGGCCAAGGGCTGGCTGGCAATGCCTAG

Protein sequence

MFSSFCSALSVLFIPNSTALPLDHLHQVSEWYEEQLEDDLKWSFAVNRILHATTSEFQEIVLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGCTARETLKHKNIEKVVMCDIDREVVNFCREHLTENQDAFQDEKLHIIFDDAKAGLEGQPEKFDVIIGDLSDPHEGGPCNHLYTKSFYEEVIKPKLNDNGIFVTQAGPAGILSHKVFSSIYNTVKHVFRYVIAYTAHVPSYADSCGWVLACDHPLKLDIEDLNNKIRERVRGELHYLDGAFIVSSTVINKTIRTLMMNETHLFTEEDARFAHGQGLAGNA

Homology

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BLAST of Tan0015157 vs. ExPASy Swiss-Prot
Match: Q9S7X6 (Thermospermine synthase ACAULIS5 OS=Arabidopsis thaliana OX=3702 GN=ACL5 PE=1 SV=1)

HSP 1 Score: 426.8 bits (1096), Expect = 2.3e-118
Identity = 199/314 (63.38%), Postives = 248/314 (78.98%), Query Frame = 0


Query: 21  PLDHLH---QVSEWYEEQLEDDLKWSFAVNRILHATTSEFQEIVLLDTKRFGKALLLDGK 80
           P   LH   Q   WYEE ++DDLKWSFA+N +LH  TSE+Q+I LLDTKRFGK L++DGK
Sbjct: 22  PTQTLHSNQQDCHWYEETIDDDLKWSFALNSVLHQGTSEYQDIALLDTKRFGKVLVIDGK 81

Query: 81  LQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGCTARETLKHKNIEKVVMCDIDRE 140
           +QSAE+DEFIYHE L+HPALL H NPKTVFIMGGGEG  ARE LKH  IEKVVMCDID+E
Sbjct: 82  MQSAERDEFIYHECLIHPALLFHPNPKTVFIMGGGEGSAAREILKHTTIEKVVMCDIDQE 141

Query: 141 VVNFCREHLTENQDAFQDEKLHIIFDDAKAGLEGQPEKFDVIIGDLSDPHEGGPCNHLYT 200
           VV+FCR  LT N DAF ++KL ++  DAKA LE + EKFD+I+GDL+DP EGGPC  LYT
Sbjct: 142 VVDFCRRFLTVNSDAFCNKKLELVIKDAKAELEKREEKFDIIVGDLADPVEGGPCYQLYT 201

Query: 201 KSFYEEVIKPKLNDNGIFVTQAGPAGILSHK-VFSSIYNTVKHVFRYVIAYTAHVPSYAD 260
           KSFY+ ++KPKL+ NGIFVTQAGPAGI +HK VF+SIYNT+K VF+YV AYTAHVPS+AD
Sbjct: 202 KSFYQNILKPKLSPNGIFVTQAGPAGIFTHKEVFTSIYNTMKQVFKYVKAYTAHVPSFAD 261

Query: 261 SCGWVLACDHPLKLDIEDLNNKIRERVRGELHYLDGAFIVSSTVINKTIRTLMMNETHLF 320
           + GWV+A DH   +++++++ +I ERV GEL YL+    VS+  +NKTI   +  ET ++
Sbjct: 262 TWGWVMASDHEFDVEVDEMDRRIEERVNGELMYLNAPSFVSAATLNKTISLALEKETEVY 321

Query: 321 TEEDARFAHGQGLA 331
           +EE+ARF HG G+A
Sbjct: 322 SEENARFIHGHGVA 335

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BLAST of Tan0015157 vs. ExPASy Swiss-Prot
Match: Q975S5 (Polyamine aminopropyltransferase OS=Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) OX=273063 GN=speE PE=3 SV=1)

HSP 1 Score: 224.2 bits (570), Expect = 2.2e-57
Identity = 116/279 (41.58%), Postives = 174/279 (62.37%), Query Frame = 0


Query: 45  AVNRILHATTSEFQEIVLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPK 104
           A+ +++    + +Q  +L++  RFGKAL+LDGK+QS   DEFIYHE+LVHP LL  +NP+
Sbjct: 20  AITKVIAEEKTPYQRALLVELARFGKALILDGKIQSTITDEFIYHEALVHPLLLSINNPE 79

Query: 105 TVFIMGGGEGCTARETLKHKNIEKVVMCDIDREVVNFCREHLTE-NQDAFQDEKLHIIFD 164
            + I+GGGEG T RE LKHK I+ V M DID  V++F +++L E +Q AF + K  ++ +
Sbjct: 80  KILILGGGEGATLREVLKHKTIKNVTMVDIDPVVIDFAKKYLQEWHQGAFDNPKSKLVIE 139

Query: 165 DAKAGLEGQPEKFDVIIGDLSDPHEGGPCNHLYTKSFYEEVIKPKLNDNGIFVTQAGPAG 224
           D    ++   EKFD ++ DL+DP +  P   LYTK FYEEV   +++  GI      P+ 
Sbjct: 140 DGYKFIKETKEKFDAVVIDLTDPIKDSPSQMLYTKEFYEEV--KRISKWGIVTQATSPS- 199

Query: 225 ILSHKVFSSIYNTVKHVFRYVIAYTAHVPSYADSCGWVLACD--HPLKLDIEDLNNKIRE 284
             S + FS IYNT+KHVF+ V A   +VP++    G+V A D  +P +   E++NN+I+E
Sbjct: 200 -FSLETFSIIYNTIKHVFKKVSAGITYVPAFDGLWGFVYASDEVNPAEFSKEEINNRIKE 259

Query: 285 RVRGELHYLDGAFIVSSTVINKTIRTLMMNETHLFTEED 321
           R+ G L + DG   ++   I K IR ++  E  + T E+
Sbjct: 260 RIDGSLRFYDGETHITMFSIPKHIREVLEKENKISTREN 294

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BLAST of Tan0015157 vs. ExPASy Swiss-Prot
Match: Q72K55 (Polyamine aminopropyltransferase OS=Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27) OX=262724 GN=speE PE=3 SV=1)

HSP 1 Score: 212.2 bits (539), Expect = 8.8e-54
Identity = 115/292 (39.38%), Postives = 172/292 (58.90%), Query Frame = 0


Query: 46  VNRILHATTSEFQEIVLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKT 105
           + R++ +  + FQ+  L ++K FGK L+LD  +QS E+DE+IYHE+LVHPA+L H  PK 
Sbjct: 21  MERVIASGKTPFQDYFLFESKGFGKVLILDKDVQSTERDEYIYHETLVHPAMLTHPEPKR 80

Query: 106 VFIMGGGEGCTARETLKHKNIEKVVMCDIDREVVNFCREHLTE-NQDAFQDEKLHIIFDD 165
           V I+GGGEG T RE LKH  +EK VM DID E+V   + H+ E +Q AF D +  ++ DD
Sbjct: 81  VLIVGGGEGATLREVLKHPTVEKAVMVDIDGELVEVAKRHMPEWHQGAFDDPRAVLVIDD 140

Query: 166 AKAGLEGQPEKFDVIIGDLSDP-HEGGPCNHLYTKSFYEEVIKPKLNDNGIFVTQAGPAG 225
           A+A LE   E++DV+I DL+DP  E  P   LYT  FY  ++K  LN  G+   QAG   
Sbjct: 141 ARAYLERTEERYDVVIIDLTDPVGEDNPARLLYTVEFY-RLVKAHLNPGGVMGMQAGMIL 200

Query: 226 ILSHKVFSSIYNTVKHVFRYVIAYTAHVPSYADSCGWVLACD--HPLKLDIEDLNNKIRE 285
           +  H+V   ++ TV+  FRYV +Y  H+P +  + G++LA D   P       +  +IRE
Sbjct: 201 LTHHRVHPVVHRTVREAFRYVRSYKNHIPGFFLNFGFLLASDAFDPAAFSEGVIEARIRE 260

Query: 286 RVRGELHYLDGAFIVSSTVINKTIRTLMMNETHLFTEEDARFAHGQGLAGNA 334
           R    L +L   ++ +  V+ K +   +  ET + T+++  +   +G A  A
Sbjct: 261 R-NLALRHLTAPYLEAMFVLPKDLLEALEKETMVSTDQNPFYVTPEGEARQA 310

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BLAST of Tan0015157 vs. ExPASy Swiss-Prot
Match: Q5SK28 (Polyamine aminopropyltransferase OS=Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) OX=300852 GN=speE PE=1 SV=1)

HSP 1 Score: 212.2 bits (539), Expect = 8.8e-54
Identity = 115/292 (39.38%), Postives = 172/292 (58.90%), Query Frame = 0


Query: 46  VNRILHATTSEFQEIVLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKT 105
           + R++ +  + FQ+  L ++K FGK L+LD  +QS E+DE+IYHE+LVHPA+L H  PK 
Sbjct: 21  MERVIASGKTPFQDYFLFESKGFGKVLILDKDVQSTERDEYIYHETLVHPAMLTHPEPKR 80

Query: 106 VFIMGGGEGCTARETLKHKNIEKVVMCDIDREVVNFCREHLTE-NQDAFQDEKLHIIFDD 165
           V I+GGGEG T RE LKH  +EK VM DID E+V   + H+ E +Q AF D +  ++ DD
Sbjct: 81  VLIVGGGEGATLREVLKHPTVEKAVMVDIDGELVEVAKRHMPEWHQGAFDDPRAVLVIDD 140

Query: 166 AKAGLEGQPEKFDVIIGDLSDP-HEGGPCNHLYTKSFYEEVIKPKLNDNGIFVTQAGPAG 225
           A+A LE   E++DV+I DL+DP  E  P   LYT  FY  ++K  LN  G+   QAG   
Sbjct: 141 ARAYLERTEERYDVVIIDLTDPVGEDNPARLLYTVEFY-RLVKAHLNPGGVMGMQAGMIL 200

Query: 226 ILSHKVFSSIYNTVKHVFRYVIAYTAHVPSYADSCGWVLACD--HPLKLDIEDLNNKIRE 285
           +  H+V   ++ TV+  FRYV +Y  H+P +  + G++LA D   P       +  +IRE
Sbjct: 201 LTHHRVHPVVHRTVREAFRYVRSYKNHIPGFFLNFGFLLASDAFDPAAFSEGVIEARIRE 260

Query: 286 RVRGELHYLDGAFIVSSTVINKTIRTLMMNETHLFTEEDARFAHGQGLAGNA 334
           R    L +L   ++ +  V+ K +   +  ET + T+++  +   +G A  A
Sbjct: 261 R-NLALRHLTAPYLEAMFVLPKDLLEALEKETMVSTDQNPFYVTPEGEARQA 310

[Close]

BLAST of Tan0015157 vs. ExPASy Swiss-Prot
Match: Q9YE02 (Polyamine aminopropyltransferase OS=Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1) OX=272557 GN=speE PE=3 SV=2)

HSP 1 Score: 209.5 bits (532), Expect = 5.7e-53
Identity = 119/299 (39.80%), Postives = 174/299 (58.19%), Query Frame = 0


Query: 31  WYEEQLEDDLKWSFAVNRILHATTSEFQEIVLLDTKRFGKALLLDGKLQSAEKDEFIYHE 90
           W+ E L  D      +  ++    S FQEI ++     GK L+LDGK QS+E DEF+YHE
Sbjct: 15  WFLEWLTPDRATLKHIEDVIFQGRSRFQEIAVVRVSGEGKVLVLDGKTQSSESDEFMYHE 74

Query: 91  SLVHPALLLHHNPKTVFIMGGGEGCTARETLKHKNIEKVVMCDIDREVVNFCREHLTE-N 150
           +LVHPA++LH +P+ V I+GGGEG T RE LKH+++EK VM DID  VVN  REHL E +
Sbjct: 75  ALVHPAMILHGSPRKVLILGGGEGATLREVLKHRSVEKAVMVDIDETVVNVAREHLREWH 134

Query: 151 QDAFQDEKLHIIFDDAKAGLEGQPEK-FDVIIGDLSDPHEGGPCNHLYTKSFYEEVIKPK 210
           + AF D +  ++ DDA   +  + E  FDV+I DL DP E GP   LY++ +Y  ++K  
Sbjct: 135 RGAFDDPRAEVVIDDAWNYVASKAETGFDVVIADLVDPLEAGPATRLYSEEYY-RMVKDV 194

Query: 211 LNDNGIFVTQAGPAGILSHKVFSSIYNTVKHVFRYVIAYTAHVPSYADSCGWVLACDHPL 270
           +N  G+FVTQA     L+ +  + I NTV  VF++V +Y  +VPS+    G+V+A D   
Sbjct: 195 MNPGGVFVTQAVSISHLT-EYHAIIRNTVARVFKHVESYGVYVPSFDSMWGFVVASDDKD 254

Query: 271 KLDIED---LNNKIRERVRG-ELHYLDGAFIVSSTVINKTIRTLMMNETHLFTEEDARF 324
              + D      ++  +++G EL +LD A ++    I K  R  +  E    T ++  F
Sbjct: 255 PRILGDRGFFETRLSHQLQGAELRFLDYASMLHMLNIPKMYREAIAREKRYATLDNQVF 311

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BLAST of Tan0015157 vs. NCBI nr
Match: XP_038890496.1 (thermospermine synthase ACAULIS5-like [Benincasa hispida])

HSP 1 Score: 650.2 bits (1676), Expect = 9.6e-183
Identity = 308/333 (92.49%), Postives = 325/333 (97.60%), Query Frame = 0


Query: 1   MFSSFCSALSVLFIPNSTALPLDHLHQVSEWYEEQLEDDLKWSFAVNRILHATTSEFQEI 60
           MFSSFCSALSVLFIPNST+LPLDH+HQVSEW+EEQLEDDLKWSFAVNRILHA TSEFQ+I
Sbjct: 1   MFSSFCSALSVLFIPNSTSLPLDHIHQVSEWFEEQLEDDLKWSFAVNRILHAATSEFQDI 60

Query: 61  VLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGCTARET 120
           VLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEG TARET
Sbjct: 61  VLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGSTARET 120

Query: 121 LKHKNIEKVVMCDIDREVVNFCREHLTENQDAFQDEKLHIIFDDAKAGLEGQPEKFDVII 180
           LKHK+IEKVVMCDIDR+VVNFCR HL ENQDAFQDEKLHIIFDDAKAGLEG+PEKFDVII
Sbjct: 121 LKHKDIEKVVMCDIDRDVVNFCRAHLKENQDAFQDEKLHIIFDDAKAGLEGRPEKFDVII 180

Query: 181 GDLSDPHEGGPCNHLYTKSFYEEVIKPKLNDNGIFVTQAGPAGILSHKVFSSIYNTVKHV 240
           GDLSDPHEGGPCNHLYTKSFYEEVIKPKLNDNGIFVTQAGPAGILSH+VFSSIYNTVKHV
Sbjct: 181 GDLSDPHEGGPCNHLYTKSFYEEVIKPKLNDNGIFVTQAGPAGILSHEVFSSIYNTVKHV 240

Query: 241 FRYVIAYTAHVPSYADSCGWVLACDHPLKLDIEDLNNKIRERVRGELHYLDGAFIVSSTV 300
           F+YVIAYTAHVPSYADSCGWVLACDHP++LD+EDLNN+IRERVRGELHYLDGA+IVSSTV
Sbjct: 241 FKYVIAYTAHVPSYADSCGWVLACDHPIELDMEDLNNRIRERVRGELHYLDGAYIVSSTV 300

Query: 301 INKTIRTLMMNETHLFTEEDARFAHGQGLAGNA 334
           +NKTIR  MMNETHLFTEEDARFAHG+GL  +A
Sbjct: 301 VNKTIRASMMNETHLFTEEDARFAHGRGLVSDA 333

[Close]

BLAST of Tan0015157 vs. NCBI nr
Match: XP_004141174.1 (thermospermine synthase ACAULIS5 [Cucumis sativus] >KGN60515.1 hypothetical protein Csa_019446 [Cucumis sativus])

HSP 1 Score: 648.7 bits (1672), Expect = 2.8e-182
Identity = 306/333 (91.89%), Postives = 326/333 (97.90%), Query Frame = 0


Query: 1   MFSSFCSALSVLFIPNSTALPLDHLHQVSEWYEEQLEDDLKWSFAVNRILHATTSEFQEI 60
           MFSSFCSALSVLF+PNS++LPLDH+ Q+ +W+EE+LEDDLKWSFAVNR+LHATTSEFQ+I
Sbjct: 1   MFSSFCSALSVLFVPNSSSLPLDHIQQLPQWFEEELEDDLKWSFAVNRVLHATTSEFQDI 60

Query: 61  VLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGCTARET 120
           VLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGCTARET
Sbjct: 61  VLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGCTARET 120

Query: 121 LKHKNIEKVVMCDIDREVVNFCREHLTENQDAFQDEKLHIIFDDAKAGLEGQPEKFDVII 180
           LKHK+IEKVVMCDIDR+VVNFCR HL ENQDAFQDE+LHIIFDDAKAGLEG+PEKFDVII
Sbjct: 121 LKHKSIEKVVMCDIDRDVVNFCRAHLKENQDAFQDERLHIIFDDAKAGLEGRPEKFDVII 180

Query: 181 GDLSDPHEGGPCNHLYTKSFYEEVIKPKLNDNGIFVTQAGPAGILSHKVFSSIYNTVKHV 240
           GDLSDPHEGGPCNHLYTKSFYE+VIKPKL+DNGIFVTQAGPAGILSHKVFSSIYNTVKHV
Sbjct: 181 GDLSDPHEGGPCNHLYTKSFYEDVIKPKLSDNGIFVTQAGPAGILSHKVFSSIYNTVKHV 240

Query: 241 FRYVIAYTAHVPSYADSCGWVLACDHPLKLDIEDLNNKIRERVRGELHYLDGAFIVSSTV 300
           FRYVIAYTAHVPSYADSCGWVLA DHP+KLDIEDLNNKIRERV+GELHYLDGAFIVSSTV
Sbjct: 241 FRYVIAYTAHVPSYADSCGWVLASDHPIKLDIEDLNNKIRERVQGELHYLDGAFIVSSTV 300

Query: 301 INKTIRTLMMNETHLFTEEDARFAHGQGLAGNA 334
           INKTIRTLMMNETH+FTEEDARFAHG+GL  NA
Sbjct: 301 INKTIRTLMMNETHVFTEEDARFAHGRGLVANA 333

[Close]

BLAST of Tan0015157 vs. NCBI nr
Match: XP_008459675.1 (PREDICTED: thermospermine synthase ACAULIS5-like [Cucumis melo] >KAA0061473.1 thermospermine synthase ACAULIS5-like [Cucumis melo var. makuwa] >TYK10801.1 thermospermine synthase ACAULIS5-like [Cucumis melo var. makuwa])

HSP 1 Score: 640.2 bits (1650), Expect = 1.0e-179
Identity = 304/333 (91.29%), Postives = 323/333 (97.00%), Query Frame = 0


Query: 1   MFSSFCSALSVLFIPNSTALPLDHLHQVSEWYEEQLEDDLKWSFAVNRILHATTSEFQEI 60
           MFSSFCSALSVLFIPNS++LPLDH+HQ+ +W+EE+LEDDLKWSFAVNR+LHATTSEFQ+I
Sbjct: 1   MFSSFCSALSVLFIPNSSSLPLDHIHQLPQWFEEELEDDLKWSFAVNRVLHATTSEFQDI 60

Query: 61  VLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGCTARET 120
           VLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEG TARET
Sbjct: 61  VLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGSTARET 120

Query: 121 LKHKNIEKVVMCDIDREVVNFCREHLTENQDAFQDEKLHIIFDDAKAGLEGQPEKFDVII 180
           LKHK+IEKVVMCDIDR+VVNFCR HL ENQDAFQDEKLHIIFDDAKAGLEG+ EKFDVII
Sbjct: 121 LKHKSIEKVVMCDIDRDVVNFCRAHLKENQDAFQDEKLHIIFDDAKAGLEGRAEKFDVII 180

Query: 181 GDLSDPHEGGPCNHLYTKSFYEEVIKPKLNDNGIFVTQAGPAGILSHKVFSSIYNTVKHV 240
           GDLSDPHEGGPCNHLYTKSFYE+VIKPKL+DNGIFVTQAGPAGILSHKVFSSIYNTVKHV
Sbjct: 181 GDLSDPHEGGPCNHLYTKSFYEDVIKPKLSDNGIFVTQAGPAGILSHKVFSSIYNTVKHV 240

Query: 241 FRYVIAYTAHVPSYADSCGWVLACDHPLKLDIEDLNNKIRERVRGELHYLDGAFIVSSTV 300
           F+YVIAYTAHVPSYADSCGWVLA DHP++LD EDLNNKI ERVRGEL YLDGAFIVSSTV
Sbjct: 241 FKYVIAYTAHVPSYADSCGWVLASDHPIRLDKEDLNNKIHERVRGELRYLDGAFIVSSTV 300

Query: 301 INKTIRTLMMNETHLFTEEDARFAHGQGLAGNA 334
           INKTIRTLMMNETH+FTEEDARFAHG+GL GNA
Sbjct: 301 INKTIRTLMMNETHVFTEEDARFAHGRGLVGNA 333

[Close]

BLAST of Tan0015157 vs. NCBI nr
Match: XP_022946737.1 (thermospermine synthase ACAULIS5-like [Cucurbita moschata])

HSP 1 Score: 591.3 bits (1523), Expect = 5.3e-165
Identity = 288/335 (85.97%), Postives = 306/335 (91.34%), Query Frame = 0


Query: 1   MFSSFCSALSVLFIP--NSTALPLDHLHQVSEWYEEQLEDDLKWSFAVNRILHATTSEFQ 60
           M S  CSAL  L IP  NSTA+P      VSEW+EEQLEDDLKWSFAVNRILHA TS+FQ
Sbjct: 1   MLSPLCSALPALSIPTSNSTAVP-----HVSEWFEEQLEDDLKWSFAVNRILHAATSKFQ 60

Query: 61  EIVLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGCTAR 120
           +IVLLDTKRFGKALLLDGKLQSAEKDEFIYHES+VHPALLLHHNPKTVFI+GGGEG TAR
Sbjct: 61  DIVLLDTKRFGKALLLDGKLQSAEKDEFIYHESMVHPALLLHHNPKTVFILGGGEGSTAR 120

Query: 121 ETLKHKNIEKVVMCDIDREVVNFCREHLTENQDAFQDEKLHIIFDDAKAGLEGQPEKFDV 180
           ETLKHK+IEKVVMCDIDR+VVNFCR HL+ENQDAFQDEKLHIIFDDAK GLEGQPEKFDV
Sbjct: 121 ETLKHKSIEKVVMCDIDRDVVNFCRAHLSENQDAFQDEKLHIIFDDAKVGLEGQPEKFDV 180

Query: 181 IIGDLSDPHEGGPCNHLYTKSFYEEVIKPKLNDNGIFVTQAGPAGILSHKVFSSIYNTVK 240
           II DL+DPHEGGPCNHLYTKSFYEE IKPKLNDNGIFVTQAGP+GILS+ VFSSIYNTVK
Sbjct: 181 IISDLADPHEGGPCNHLYTKSFYEEGIKPKLNDNGIFVTQAGPSGILSYNVFSSIYNTVK 240

Query: 241 HVFRYVIAYTAHVPSYADSCGWVLACDHPLKLDIEDLNNKIRERVRGELHYLDGAFIVSS 300
           HVF+YVIAYTAH+PSYADS GWVLA DHP KLDIEDL+ +IRERVRGEL YLDGAFIVSS
Sbjct: 241 HVFKYVIAYTAHIPSYADSSGWVLASDHPFKLDIEDLDKRIRERVRGELRYLDGAFIVSS 300

Query: 301 TVINKTIRTLMMNETHLFTEEDARFAHGQGLAGNA 334
           TVINKTIRT MMNETH+FTEEDARF HGQGLA +A
Sbjct: 301 TVINKTIRTAMMNETHVFTEEDARFTHGQGLASHA 330

[Close]

BLAST of Tan0015157 vs. NCBI nr
Match: KAG7030499.1 (Thermospermine synthase ACAULIS5 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 590.5 bits (1521), Expect = 9.1e-165
Identity = 286/335 (85.37%), Postives = 306/335 (91.34%), Query Frame = 0


Query: 1   MFSSFCSALSVLFIP--NSTALPLDHLHQVSEWYEEQLEDDLKWSFAVNRILHATTSEFQ 60
           M S  CSAL  L IP  NSTA+P      VSEW+EEQLEDDLKWSFAVNRILHA TS+FQ
Sbjct: 1   MLSPLCSALPALSIPTSNSTAVP-----HVSEWFEEQLEDDLKWSFAVNRILHAATSKFQ 60

Query: 61  EIVLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGCTAR 120
           +IVLLDTKRFGKALLLDGKLQSAEKDEFIYHES+VHPALLLHHNPKTVFI+GGGEG TAR
Sbjct: 61  DIVLLDTKRFGKALLLDGKLQSAEKDEFIYHESMVHPALLLHHNPKTVFILGGGEGSTAR 120

Query: 121 ETLKHKNIEKVVMCDIDREVVNFCREHLTENQDAFQDEKLHIIFDDAKAGLEGQPEKFDV 180
           ETLKHK+IEKVVMCDIDR+VVNFCR HL+ENQDAFQDEKLHIIFDDAK GLEGQPEKFD+
Sbjct: 121 ETLKHKSIEKVVMCDIDRDVVNFCRAHLSENQDAFQDEKLHIIFDDAKVGLEGQPEKFDI 180

Query: 181 IIGDLSDPHEGGPCNHLYTKSFYEEVIKPKLNDNGIFVTQAGPAGILSHKVFSSIYNTVK 240
           II DL+DPHEGGPCNHLYTKSFYEE IKPKLNDNGIFVTQAGP+GILS+ VFSSIYNTVK
Sbjct: 181 IISDLADPHEGGPCNHLYTKSFYEEGIKPKLNDNGIFVTQAGPSGILSYNVFSSIYNTVK 240

Query: 241 HVFRYVIAYTAHVPSYADSCGWVLACDHPLKLDIEDLNNKIRERVRGELHYLDGAFIVSS 300
           HVF+YVIAYTAH+PSYADS GWVLA DHP KLD+EDL+ +IRERVRGEL YLDGAFIVSS
Sbjct: 241 HVFKYVIAYTAHIPSYADSSGWVLASDHPFKLDVEDLDKRIRERVRGELRYLDGAFIVSS 300

Query: 301 TVINKTIRTLMMNETHLFTEEDARFAHGQGLAGNA 334
           TVINKTIRT MMNETH+FTEEDARF HGQGLA +A
Sbjct: 301 TVINKTIRTAMMNETHVFTEEDARFTHGQGLASHA 330

[Close]

BLAST of Tan0015157 vs. ExPASy TrEMBL
Match: A0A0A0LET5 (PABS domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G000240 PE=3 SV=1)

HSP 1 Score: 648.7 bits (1672), Expect = 1.4e-182
Identity = 306/333 (91.89%), Postives = 326/333 (97.90%), Query Frame = 0


Query: 1   MFSSFCSALSVLFIPNSTALPLDHLHQVSEWYEEQLEDDLKWSFAVNRILHATTSEFQEI 60
           MFSSFCSALSVLF+PNS++LPLDH+ Q+ +W+EE+LEDDLKWSFAVNR+LHATTSEFQ+I
Sbjct: 1   MFSSFCSALSVLFVPNSSSLPLDHIQQLPQWFEEELEDDLKWSFAVNRVLHATTSEFQDI 60

Query: 61  VLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGCTARET 120
           VLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGCTARET
Sbjct: 61  VLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGCTARET 120

Query: 121 LKHKNIEKVVMCDIDREVVNFCREHLTENQDAFQDEKLHIIFDDAKAGLEGQPEKFDVII 180
           LKHK+IEKVVMCDIDR+VVNFCR HL ENQDAFQDE+LHIIFDDAKAGLEG+PEKFDVII
Sbjct: 121 LKHKSIEKVVMCDIDRDVVNFCRAHLKENQDAFQDERLHIIFDDAKAGLEGRPEKFDVII 180

Query: 181 GDLSDPHEGGPCNHLYTKSFYEEVIKPKLNDNGIFVTQAGPAGILSHKVFSSIYNTVKHV 240
           GDLSDPHEGGPCNHLYTKSFYE+VIKPKL+DNGIFVTQAGPAGILSHKVFSSIYNTVKHV
Sbjct: 181 GDLSDPHEGGPCNHLYTKSFYEDVIKPKLSDNGIFVTQAGPAGILSHKVFSSIYNTVKHV 240

Query: 241 FRYVIAYTAHVPSYADSCGWVLACDHPLKLDIEDLNNKIRERVRGELHYLDGAFIVSSTV 300
           FRYVIAYTAHVPSYADSCGWVLA DHP+KLDIEDLNNKIRERV+GELHYLDGAFIVSSTV
Sbjct: 241 FRYVIAYTAHVPSYADSCGWVLASDHPIKLDIEDLNNKIRERVQGELHYLDGAFIVSSTV 300

Query: 301 INKTIRTLMMNETHLFTEEDARFAHGQGLAGNA 334
           INKTIRTLMMNETH+FTEEDARFAHG+GL  NA
Sbjct: 301 INKTIRTLMMNETHVFTEEDARFAHGRGLVANA 333

[Close]

BLAST of Tan0015157 vs. ExPASy TrEMBL
Match: A0A5A7UZV1 (Thermospermine synthase ACAULIS5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold332G001170 PE=3 SV=1)

HSP 1 Score: 640.2 bits (1650), Expect = 4.8e-180
Identity = 304/333 (91.29%), Postives = 323/333 (97.00%), Query Frame = 0


Query: 1   MFSSFCSALSVLFIPNSTALPLDHLHQVSEWYEEQLEDDLKWSFAVNRILHATTSEFQEI 60
           MFSSFCSALSVLFIPNS++LPLDH+HQ+ +W+EE+LEDDLKWSFAVNR+LHATTSEFQ+I
Sbjct: 1   MFSSFCSALSVLFIPNSSSLPLDHIHQLPQWFEEELEDDLKWSFAVNRVLHATTSEFQDI 60

Query: 61  VLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGCTARET 120
           VLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEG TARET
Sbjct: 61  VLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGSTARET 120

Query: 121 LKHKNIEKVVMCDIDREVVNFCREHLTENQDAFQDEKLHIIFDDAKAGLEGQPEKFDVII 180
           LKHK+IEKVVMCDIDR+VVNFCR HL ENQDAFQDEKLHIIFDDAKAGLEG+ EKFDVII
Sbjct: 121 LKHKSIEKVVMCDIDRDVVNFCRAHLKENQDAFQDEKLHIIFDDAKAGLEGRAEKFDVII 180

Query: 181 GDLSDPHEGGPCNHLYTKSFYEEVIKPKLNDNGIFVTQAGPAGILSHKVFSSIYNTVKHV 240
           GDLSDPHEGGPCNHLYTKSFYE+VIKPKL+DNGIFVTQAGPAGILSHKVFSSIYNTVKHV
Sbjct: 181 GDLSDPHEGGPCNHLYTKSFYEDVIKPKLSDNGIFVTQAGPAGILSHKVFSSIYNTVKHV 240

Query: 241 FRYVIAYTAHVPSYADSCGWVLACDHPLKLDIEDLNNKIRERVRGELHYLDGAFIVSSTV 300
           F+YVIAYTAHVPSYADSCGWVLA DHP++LD EDLNNKI ERVRGEL YLDGAFIVSSTV
Sbjct: 241 FKYVIAYTAHVPSYADSCGWVLASDHPIRLDKEDLNNKIHERVRGELRYLDGAFIVSSTV 300

Query: 301 INKTIRTLMMNETHLFTEEDARFAHGQGLAGNA 334
           INKTIRTLMMNETH+FTEEDARFAHG+GL GNA
Sbjct: 301 INKTIRTLMMNETHVFTEEDARFAHGRGLVGNA 333

[Close]

BLAST of Tan0015157 vs. ExPASy TrEMBL
Match: A0A1S3CAR3 (thermospermine synthase ACAULIS5-like OS=Cucumis melo OX=3656 GN=LOC103498720 PE=3 SV=1)

HSP 1 Score: 640.2 bits (1650), Expect = 4.8e-180
Identity = 304/333 (91.29%), Postives = 323/333 (97.00%), Query Frame = 0


Query: 1   MFSSFCSALSVLFIPNSTALPLDHLHQVSEWYEEQLEDDLKWSFAVNRILHATTSEFQEI 60
           MFSSFCSALSVLFIPNS++LPLDH+HQ+ +W+EE+LEDDLKWSFAVNR+LHATTSEFQ+I
Sbjct: 1   MFSSFCSALSVLFIPNSSSLPLDHIHQLPQWFEEELEDDLKWSFAVNRVLHATTSEFQDI 60

Query: 61  VLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGCTARET 120
           VLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEG TARET
Sbjct: 61  VLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGSTARET 120

Query: 121 LKHKNIEKVVMCDIDREVVNFCREHLTENQDAFQDEKLHIIFDDAKAGLEGQPEKFDVII 180
           LKHK+IEKVVMCDIDR+VVNFCR HL ENQDAFQDEKLHIIFDDAKAGLEG+ EKFDVII
Sbjct: 121 LKHKSIEKVVMCDIDRDVVNFCRAHLKENQDAFQDEKLHIIFDDAKAGLEGRAEKFDVII 180

Query: 181 GDLSDPHEGGPCNHLYTKSFYEEVIKPKLNDNGIFVTQAGPAGILSHKVFSSIYNTVKHV 240
           GDLSDPHEGGPCNHLYTKSFYE+VIKPKL+DNGIFVTQAGPAGILSHKVFSSIYNTVKHV
Sbjct: 181 GDLSDPHEGGPCNHLYTKSFYEDVIKPKLSDNGIFVTQAGPAGILSHKVFSSIYNTVKHV 240

Query: 241 FRYVIAYTAHVPSYADSCGWVLACDHPLKLDIEDLNNKIRERVRGELHYLDGAFIVSSTV 300
           F+YVIAYTAHVPSYADSCGWVLA DHP++LD EDLNNKI ERVRGEL YLDGAFIVSSTV
Sbjct: 241 FKYVIAYTAHVPSYADSCGWVLASDHPIRLDKEDLNNKIHERVRGELRYLDGAFIVSSTV 300

Query: 301 INKTIRTLMMNETHLFTEEDARFAHGQGLAGNA 334
           INKTIRTLMMNETH+FTEEDARFAHG+GL GNA
Sbjct: 301 INKTIRTLMMNETHVFTEEDARFAHGRGLVGNA 333

[Close]

BLAST of Tan0015157 vs. ExPASy TrEMBL
Match: A0A6J1G4J8 (thermospermine synthase ACAULIS5-like OS=Cucurbita moschata OX=3662 GN=LOC111450716 PE=3 SV=1)

HSP 1 Score: 591.3 bits (1523), Expect = 2.6e-165
Identity = 288/335 (85.97%), Postives = 306/335 (91.34%), Query Frame = 0


Query: 1   MFSSFCSALSVLFIP--NSTALPLDHLHQVSEWYEEQLEDDLKWSFAVNRILHATTSEFQ 60
           M S  CSAL  L IP  NSTA+P      VSEW+EEQLEDDLKWSFAVNRILHA TS+FQ
Sbjct: 1   MLSPLCSALPALSIPTSNSTAVP-----HVSEWFEEQLEDDLKWSFAVNRILHAATSKFQ 60

Query: 61  EIVLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGCTAR 120
           +IVLLDTKRFGKALLLDGKLQSAEKDEFIYHES+VHPALLLHHNPKTVFI+GGGEG TAR
Sbjct: 61  DIVLLDTKRFGKALLLDGKLQSAEKDEFIYHESMVHPALLLHHNPKTVFILGGGEGSTAR 120

Query: 121 ETLKHKNIEKVVMCDIDREVVNFCREHLTENQDAFQDEKLHIIFDDAKAGLEGQPEKFDV 180
           ETLKHK+IEKVVMCDIDR+VVNFCR HL+ENQDAFQDEKLHIIFDDAK GLEGQPEKFDV
Sbjct: 121 ETLKHKSIEKVVMCDIDRDVVNFCRAHLSENQDAFQDEKLHIIFDDAKVGLEGQPEKFDV 180

Query: 181 IIGDLSDPHEGGPCNHLYTKSFYEEVIKPKLNDNGIFVTQAGPAGILSHKVFSSIYNTVK 240
           II DL+DPHEGGPCNHLYTKSFYEE IKPKLNDNGIFVTQAGP+GILS+ VFSSIYNTVK
Sbjct: 181 IISDLADPHEGGPCNHLYTKSFYEEGIKPKLNDNGIFVTQAGPSGILSYNVFSSIYNTVK 240

Query: 241 HVFRYVIAYTAHVPSYADSCGWVLACDHPLKLDIEDLNNKIRERVRGELHYLDGAFIVSS 300
           HVF+YVIAYTAH+PSYADS GWVLA DHP KLDIEDL+ +IRERVRGEL YLDGAFIVSS
Sbjct: 241 HVFKYVIAYTAHIPSYADSSGWVLASDHPFKLDIEDLDKRIRERVRGELRYLDGAFIVSS 300

Query: 301 TVINKTIRTLMMNETHLFTEEDARFAHGQGLAGNA 334
           TVINKTIRT MMNETH+FTEEDARF HGQGLA +A
Sbjct: 301 TVINKTIRTAMMNETHVFTEEDARFTHGQGLASHA 330

[Close]

BLAST of Tan0015157 vs. ExPASy TrEMBL
Match: A0A6J1KJ05 (thermospermine synthase ACAULIS5-like OS=Cucurbita maxima OX=3661 GN=LOC111493662 PE=3 SV=1)

HSP 1 Score: 586.3 bits (1510), Expect = 8.3e-164
Identity = 285/335 (85.07%), Postives = 306/335 (91.34%), Query Frame = 0


Query: 1   MFSSFCSALSVLFIP--NSTALPLDHLHQVSEWYEEQLEDDLKWSFAVNRILHATTSEFQ 60
           M S  CSAL  L IP  NSTA+P     QVSEW+EEQLEDDLKWSFAV RILHA TS+FQ
Sbjct: 1   MLSPLCSALPALSIPTSNSTAVP-----QVSEWFEEQLEDDLKWSFAVTRILHAATSKFQ 60

Query: 61  EIVLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGCTAR 120
           +IVLLDTKRFGKALLLDGKLQSAEKDEFIYHES+VHPALLLHHNPKTVFI+GGGEG TAR
Sbjct: 61  DIVLLDTKRFGKALLLDGKLQSAEKDEFIYHESMVHPALLLHHNPKTVFILGGGEGSTAR 120

Query: 121 ETLKHKNIEKVVMCDIDREVVNFCREHLTENQDAFQDEKLHIIFDDAKAGLEGQPEKFDV 180
           ETLKHK+IEKVVMCDIDR+VVNFCR HL+ENQDAFQDEKLHIIF+DAK GLEGQPEKFDV
Sbjct: 121 ETLKHKSIEKVVMCDIDRDVVNFCRAHLSENQDAFQDEKLHIIFNDAKVGLEGQPEKFDV 180

Query: 181 IIGDLSDPHEGGPCNHLYTKSFYEEVIKPKLNDNGIFVTQAGPAGILSHKVFSSIYNTVK 240
           II DL+DPHEGGPCNHLYTKSFYEE IKPKLNDNGIFVTQAGP+GILS+ VFSSIYNTVK
Sbjct: 181 IISDLADPHEGGPCNHLYTKSFYEEGIKPKLNDNGIFVTQAGPSGILSYNVFSSIYNTVK 240

Query: 241 HVFRYVIAYTAHVPSYADSCGWVLACDHPLKLDIEDLNNKIRERVRGELHYLDGAFIVSS 300
           HVF+YVIAYTAH+PSYADS GWVLA DHP +LD+EDL+ +IRERVRGEL YLDGAFIVSS
Sbjct: 241 HVFKYVIAYTAHIPSYADSSGWVLASDHPFELDMEDLDKRIRERVRGELRYLDGAFIVSS 300

Query: 301 TVINKTIRTLMMNETHLFTEEDARFAHGQGLAGNA 334
           TVINKTIRT MMNETH+FTEEDARF HGQGLA +A
Sbjct: 301 TVINKTIRTAMMNETHVFTEEDARFTHGQGLASHA 330

[Close]

BLAST of Tan0015157 vs. TAIR 10
Match: AT5G19530.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 426.8 bits (1096), Expect = 1.6e-119
Identity = 199/314 (63.38%), Postives = 248/314 (78.98%), Query Frame = 0


Query: 21  PLDHLH---QVSEWYEEQLEDDLKWSFAVNRILHATTSEFQEIVLLDTKRFGKALLLDGK 80
           P   LH   Q   WYEE ++DDLKWSFA+N +LH  TSE+Q+I LLDTKRFGK L++DGK
Sbjct: 22  PTQTLHSNQQDCHWYEETIDDDLKWSFALNSVLHQGTSEYQDIALLDTKRFGKVLVIDGK 81

Query: 81  LQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGCTARETLKHKNIEKVVMCDIDRE 140
           +QSAE+DEFIYHE L+HPALL H NPKTVFIMGGGEG  ARE LKH  IEKVVMCDID+E
Sbjct: 82  MQSAERDEFIYHECLIHPALLFHPNPKTVFIMGGGEGSAAREILKHTTIEKVVMCDIDQE 141

Query: 141 VVNFCREHLTENQDAFQDEKLHIIFDDAKAGLEGQPEKFDVIIGDLSDPHEGGPCNHLYT 200
           VV+FCR  LT N DAF ++KL ++  DAKA LE + EKFD+I+GDL+DP EGGPC  LYT
Sbjct: 142 VVDFCRRFLTVNSDAFCNKKLELVIKDAKAELEKREEKFDIIVGDLADPVEGGPCYQLYT 201

Query: 201 KSFYEEVIKPKLNDNGIFVTQAGPAGILSHK-VFSSIYNTVKHVFRYVIAYTAHVPSYAD 260
           KSFY+ ++KPKL+ NGIFVTQAGPAGI +HK VF+SIYNT+K VF+YV AYTAHVPS+AD
Sbjct: 202 KSFYQNILKPKLSPNGIFVTQAGPAGIFTHKEVFTSIYNTMKQVFKYVKAYTAHVPSFAD 261

Query: 261 SCGWVLACDHPLKLDIEDLNNKIRERVRGELHYLDGAFIVSSTVINKTIRTLMMNETHLF 320
           + GWV+A DH   +++++++ +I ERV GEL YL+    VS+  +NKTI   +  ET ++
Sbjct: 262 TWGWVMASDHEFDVEVDEMDRRIEERVNGELMYLNAPSFVSAATLNKTISLALEKETEVY 321

Query: 321 TEEDARFAHGQGLA 331
           +EE+ARF HG G+A
Sbjct: 322 SEENARFIHGHGVA 335

[Close]

BLAST of Tan0015157 vs. TAIR 10
Match: AT5G19530.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 426.8 bits (1096), Expect = 1.6e-119
Identity = 199/314 (63.38%), Postives = 248/314 (78.98%), Query Frame = 0


Query: 21  PLDHLH---QVSEWYEEQLEDDLKWSFAVNRILHATTSEFQEIVLLDTKRFGKALLLDGK 80
           P   LH   Q   WYEE ++DDLKWSFA+N +LH  TSE+Q+I LLDTKRFGK L++DGK
Sbjct: 15  PTQTLHSNQQDCHWYEETIDDDLKWSFALNSVLHQGTSEYQDIALLDTKRFGKVLVIDGK 74

Query: 81  LQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGCTARETLKHKNIEKVVMCDIDRE 140
           +QSAE+DEFIYHE L+HPALL H NPKTVFIMGGGEG  ARE LKH  IEKVVMCDID+E
Sbjct: 75  MQSAERDEFIYHECLIHPALLFHPNPKTVFIMGGGEGSAAREILKHTTIEKVVMCDIDQE 134

Query: 141 VVNFCREHLTENQDAFQDEKLHIIFDDAKAGLEGQPEKFDVIIGDLSDPHEGGPCNHLYT 200
           VV+FCR  LT N DAF ++KL ++  DAKA LE + EKFD+I+GDL+DP EGGPC  LYT
Sbjct: 135 VVDFCRRFLTVNSDAFCNKKLELVIKDAKAELEKREEKFDIIVGDLADPVEGGPCYQLYT 194

Query: 201 KSFYEEVIKPKLNDNGIFVTQAGPAGILSHK-VFSSIYNTVKHVFRYVIAYTAHVPSYAD 260
           KSFY+ ++KPKL+ NGIFVTQAGPAGI +HK VF+SIYNT+K VF+YV AYTAHVPS+AD
Sbjct: 195 KSFYQNILKPKLSPNGIFVTQAGPAGIFTHKEVFTSIYNTMKQVFKYVKAYTAHVPSFAD 254

Query: 261 SCGWVLACDHPLKLDIEDLNNKIRERVRGELHYLDGAFIVSSTVINKTIRTLMMNETHLF 320
           + GWV+A DH   +++++++ +I ERV GEL YL+    VS+  +NKTI   +  ET ++
Sbjct: 255 TWGWVMASDHEFDVEVDEMDRRIEERVNGELMYLNAPSFVSAATLNKTISLALEKETEVY 314

Query: 321 TEEDARFAHGQGLA 331
           +EE+ARF HG G+A
Sbjct: 315 SEENARFIHGHGVA 328

[Close]

BLAST of Tan0015157 vs. TAIR 10
Match: AT1G23820.1 (spermidine synthase 1 )

HSP 1 Score: 128.6 bits (322), Expect = 9.1e-30
Identity = 73/242 (30.17%), Postives = 126/242 (52.07%), Query Frame = 0


Query: 43  SFAVNRILHATTSEFQEIVLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHN 102
           S  V ++L    S++Q++++  +  +GK L+LDG +Q  E+DE  Y E + H  L    N
Sbjct: 105 SLKVEKVLFQGKSDYQDVIVFQSATYGKVLVLDGVIQLTERDECAYQEMITHLPLCSIPN 164

Query: 103 PKTVFIMGGGEGCTARETLKHKNIEKVVMCDIDREVVNFCREHLTENQDAFQDEKLHIIF 162
           PK V ++GGG+G   RE  +H +IE++ MC+ID+ VV+  ++   +    ++D +++++ 
Sbjct: 165 PKKVLVIGGGDGGVLREVARHASIEQIDMCEIDKMVVDVSKQFFPDVAIGYEDPRVNLVI 224

Query: 163 DDAKAGLEGQPE-KFDVIIGDLSDPHEGGPCNHLYTKSFYEEVIKPKLNDNGIFVTQAGP 222
            D  A L+   E  +D +I D SDP   GP   L+ K F++ V +  L   G+  TQA  
Sbjct: 225 GDGVAFLKNAAEGSYDAVIVDSSDPI--GPAKELFEKPFFQSVAR-ALRPGGVVCTQAES 284

Query: 223 AGILSHKVFSSIYNTVKHVFRYVIAYT-AHVPSYADS-CGWVLACDHPLKLDIEDLNNKI 282
              L   +   I +  + +F+  + Y    VP+Y     G++L       +D +   N I
Sbjct: 285 LW-LHMDIIEDIVSNCREIFKGSVNYAWTSVPTYPSGVIGFMLCSTEGPDVDFKHPLNPI 342

[Close]

BLAST of Tan0015157 vs. TAIR 10
Match: AT1G23820.2 (spermidine synthase 1 )

HSP 1 Score: 128.3 bits (321), Expect = 1.2e-29
Identity = 70/224 (31.25%), Postives = 120/224 (53.57%), Query Frame = 0


Query: 43  SFAVNRILHATTSEFQEIVLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHN 102
           S  V ++L    S++Q++++  +  +GK L+LDG +Q  E+DE  Y E + H  L    N
Sbjct: 105 SLKVEKVLFQGKSDYQDVIVFQSATYGKVLVLDGVIQLTERDECAYQEMITHLPLCSIPN 164

Query: 103 PKTVFIMGGGEGCTARETLKHKNIEKVVMCDIDREVVNFCREHLTENQDAFQDEKLHIIF 162
           PK V ++GGG+G   RE  +H +IE++ MC+ID+ VV+  ++   +    ++D +++++ 
Sbjct: 165 PKKVLVIGGGDGGVLREVARHASIEQIDMCEIDKMVVDVSKQFFPDVAIGYEDPRVNLVI 224

Query: 163 DDAKAGLEGQPE-KFDVIIGDLSDPHEGGPCNHLYTKSFYEEVIKPKLNDNGIFVTQAGP 222
            D  A L+   E  +D +I D SDP   GP   L+ K F++ V +  L   G+  TQA  
Sbjct: 225 GDGVAFLKNAAEGSYDAVIVDSSDPI--GPAKELFEKPFFQSVAR-ALRPGGVVCTQAES 284

Query: 223 AGILSHKVFSSIYNTVKHVFRYVIAYT-AHVPSYADSCG-WVLA 264
              L   +   I +  + +F+  + Y    VP+Y    G W+ A
Sbjct: 285 LW-LHMDIIEDIVSNCREIFKGSVNYAWTSVPTYPRHWGHWIYA 324

[Close]

BLAST of Tan0015157 vs. TAIR 10
Match: AT1G70310.1 (spermidine synthase 2 )

HSP 1 Score: 126.7 bits (317), Expect = 3.5e-29
Identity = 69/224 (30.80%), Postives = 118/224 (52.68%), Query Frame = 0


Query: 43  SFAVNRILHATTSEFQEIVLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHN 102
           S  V +IL    S++Q++++  +  +GK L+LDG +Q  E+DE  Y E + H  L    N
Sbjct: 65  SLKVEKILFQGKSDYQDVIVFQSATYGKVLVLDGVIQLTERDECAYQEMITHLPLCSISN 124

Query: 103 PKTVFIMGGGEGCTARETLKHKNIEKVVMCDIDREVVNFCREHLTENQDAFQDEKLHIIF 162
           PK V ++GGG+G   RE  +H ++E++ +C+ID+ VV+  +++       ++D ++++I 
Sbjct: 125 PKKVLVIGGGDGGVLREVARHSSVEQIDICEIDKMVVDVAKQYFPNVAVGYEDPRVNLII 184

Query: 163 DDAKAGLEGQPE-KFDVIIGDLSDPHEGGPCNHLYTKSFYEEVIKPKLNDNGIFVTQAGP 222
            D  A L+   E  +D +I D SDP   GP   L+ K F+E V +  L   G+  TQA  
Sbjct: 185 GDGVAFLKNAAEGTYDAVIVDSSDPI--GPAKELFEKPFFESVNR-ALRPGGVVCTQAES 244

Query: 223 AGILSHKVFSSIYNTVKHVFRYVIAYT-AHVPSYADSCGWVLAC 265
              L   +   I +  + +F+  + Y    VP+Y       + C
Sbjct: 245 LW-LHMDIIEDIVSNCRDIFKGSVNYAWTSVPTYPSGVIGFMLC 284

The following BLAST results are available for this feature:

BLAST of Tan0015157 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9S7X6	2.3e-118	63.38	Thermospermine synthase ACAULIS5 OS=Arabidopsis thaliana OX=3702 GN=ACL5 PE=1 SV...	[more]
Q975S5	2.2e-57	41.58	Polyamine aminopropyltransferase OS=Sulfurisphaera tokodaii (strain DSM 16993 / ...	[more]
Q72K55	8.8e-54	39.38	Polyamine aminopropyltransferase OS=Thermus thermophilus (strain ATCC BAA-163 / ...	[more]
Q5SK28	8.8e-54	39.38	Polyamine aminopropyltransferase OS=Thermus thermophilus (strain ATCC 27634 / DS...	[more]
Q9YE02	5.7e-53	39.80	Polyamine aminopropyltransferase OS=Aeropyrum pernix (strain ATCC 700893 / DSM 1...	[more]

BLAST of Tan0015157 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038890496.1	9.6e-183	92.49	thermospermine synthase ACAULIS5-like [Benincasa hispida]	[more]
XP_004141174.1	2.8e-182	91.89	thermospermine synthase ACAULIS5 [Cucumis sativus] >KGN60515.1 hypothetical prot...	[more]
XP_008459675.1	1.0e-179	91.29	PREDICTED: thermospermine synthase ACAULIS5-like [Cucumis melo] >KAA0061473.1 th...	[more]
XP_022946737.1	5.3e-165	85.97	thermospermine synthase ACAULIS5-like [Cucurbita moschata]	[more]
KAG7030499.1	9.1e-165	85.37	Thermospermine synthase ACAULIS5 [Cucurbita argyrosperma subsp. argyrosperma]	[more]

BLAST of Tan0015157 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LET5	1.4e-182	91.89	PABS domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G000240 PE=3 S...	[more]
A0A5A7UZV1	4.8e-180	91.29	Thermospermine synthase ACAULIS5-like OS=Cucumis melo var. makuwa OX=1194695 GN=...	[more]
A0A1S3CAR3	4.8e-180	91.29	thermospermine synthase ACAULIS5-like OS=Cucumis melo OX=3656 GN=LOC103498720 PE...	[more]
A0A6J1G4J8	2.6e-165	85.97	thermospermine synthase ACAULIS5-like OS=Cucurbita moschata OX=3662 GN=LOC111450...	[more]
A0A6J1KJ05	8.3e-164	85.07	thermospermine synthase ACAULIS5-like OS=Cucurbita maxima OX=3661 GN=LOC11149366...	[more]

BLAST of Tan0015157 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G19530.1	1.6e-119	63.38	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT5G19530.2	1.6e-119	63.38	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT1G23820.1	9.1e-30	30.17	spermidine synthase 1	[more]
AT1G23820.2	1.2e-29	31.25	spermidine synthase 1	[more]
AT1G70310.1	3.5e-29	30.80	spermidine synthase 2	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR035246	Spermidine synthase, tetramerisation domain	PFAM	PF17284	Spermine_synt_N	coord: 31..82 e-value: 4.5E-12 score: 45.7
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 83..332 e-value: 3.2E-73 score: 248.3
None	No IPR available	PFAM	PF01564	Spermine_synth	coord: 85..257 e-value: 2.6E-38 score: 131.3
None	No IPR available	PANTHER	PTHR43317	THERMOSPERMINE SYNTHASE ACAULIS5	coord: 3..329
None	No IPR available	PANTHER	PTHR43317:SF6	THERMOSPERMINE SYNTHASE ACAULIS5-LIKE	coord: 3..329
None	No IPR available	CDD	cd02440	AdoMet_MTases	coord: 110..218 e-value: 5.53618E-7 score: 45.4987
IPR037163	Spermidine synthase, tetramerisation domain superfamily	GENE3D	2.30.140.10	Spermidine synthase, tetramerisation domain	coord: 28..82 e-value: 6.8E-16 score: 60.4
IPR001045	Spermidine/spermine synthases	HAMAP	MF_00198	Spermidine_synth	coord: 30..306 score: 30.326389
IPR030374	Polyamine biosynthesis domain	PROSITE	PS51006	PABS_2	coord: 29..265 score: 88.354675
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 29..307

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Tan0015157.1	Tan0015157.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032259	methylation
biological_process	GO:0006596	polyamine biosynthetic process
molecular_function	GO:0008168	methyltransferase activity
molecular_function	GO:0010487	thermospermine synthase activity
molecular_function	GO:0003824	catalytic activity