Tan0011175 (gene) Snake gourd v1

Overview


Name	Tan0011175
Type	gene
Organism	Trichosanthes anguina (Snake gourd v1)
Description	rRNA N-glycosidase
Location	LG06: 30437547 .. 30438443 (+)
RNA-Seq Expression	Tan0011175
Synteny	Tan0011175

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAACAAATTCATAGCACTCTCCTTTTTCTTTCTCGTCATCTCTCTTGGCTCTCCTACTGCCAATGGTGATGTTAGCTTCGACTTGTCGACAGCAACTCAAAGTTCCTATTCATCTTTCATCACACAGCTAAGGGATGCTCTTCCAACTCAAGGAACAGTGTGCGGCATTCCTTTGCTGCCTTCCACCGCATCAGGCTTACAATGGTTCACATTCTTCAATCTTACCAATTATAACGATGAAACCATCACCGTGGCTGTAAATGTAACCAATGTCTACATCGTGGCGTATCGTACCAATTCTGCATCCTACTTTTTTGAAGACACTTCCGCTGAAGCTTACGGGCTCTTATTTTCCGGTACTAAAAAGATAAATCTATCCTATTCCAGTAATTATGATAAGCTTCAAAGCGTTGTAGGCAAACAAAGAGATATGATTGAGCTTGGAATCCCGGCTTTAAGCAGTGCCATTTCAAACATGGTTTACTACAAATACCAACTTACTGCAGCAGCTCTTCTTGTGCTCATTCAGTCCACTGCAGAAGCCGCAAGGTATAAATATATTGAGCAACAAGTTTCTTCACATATTAGGGATAATTTTTATCCAAATCAAGCAGTCATAAGCTTAGAAAACAATTGGGGTGCTCTTTCCAAACAAATCAAGATAGCAAATAGTACAGGAAGCGGACAATTTGAAAGTGCTGTTGAGCTTATGACCCCTAATGGCACACGAGTTAGTGTAACCAATACTTCAGCTGGAGTTGTAAAAGGAAATATCAAACTCCTACTATACTCTAAAGTCAGTGTTGGTAGTGAAAATGATATCCCTACTACAATCTTGCACCCTGGAGCTATGGGAATGCTTCACAATCAGAATGGAAATTATGTTACAATGTAA

mRNA sequence

ATGAACAAATTCATAGCACTCTCCTTTTTCTTTCTCGTCATCTCTCTTGGCTCTCCTACTGCCAATGGTGATGTTAGCTTCGACTTGTCGACAGCAACTCAAAGTTCCTATTCATCTTTCATCACACAGCTAAGGGATGCTCTTCCAACTCAAGGAACAGTGTGCGGCATTCCTTTGCTGCCTTCCACCGCATCAGGCTTACAATGGTTCACATTCTTCAATCTTACCAATTATAACGATGAAACCATCACCGTGGCTGTAAATGTAACCAATGTCTACATCGTGGCGTATCGTACCAATTCTGCATCCTACTTTTTTGAAGACACTTCCGCTGAAGCTTACGGGCTCTTATTTTCCGGTACTAAAAAGATAAATCTATCCTATTCCAGTAATTATGATAAGCTTCAAAGCGTTGTAGGCAAACAAAGAGATATGATTGAGCTTGGAATCCCGGCTTTAAGCAGTGCCATTTCAAACATGGTTTACTACAAATACCAACTTACTGCAGCAGCTCTTCTTGTGCTCATTCAGTCCACTGCAGAAGCCGCAAGGTATAAATATATTGAGCAACAAGTTTCTTCACATATTAGGGATAATTTTTATCCAAATCAAGCAGTCATAAGCTTAGAAAACAATTGGGGTGCTCTTTCCAAACAAATCAAGATAGCAAATAGTACAGGAAGCGGACAATTTGAAAGTGCTGTTGAGCTTATGACCCCTAATGGCACACGAGTTAGTGTAACCAATACTTCAGCTGGAGTTGTAAAAGGAAATATCAAACTCCTACTATACTCTAAAGTCAGTGTTGGTAGTGAAAATGATATCCCTACTACAATCTTGCACCCTGGAGCTATGGGAATGCTTCACAATCAGAATGGAAATTATGTTACAATGTAA

Coding sequence (CDS)

ATGAACAAATTCATAGCACTCTCCTTTTTCTTTCTCGTCATCTCTCTTGGCTCTCCTACTGCCAATGGTGATGTTAGCTTCGACTTGTCGACAGCAACTCAAAGTTCCTATTCATCTTTCATCACACAGCTAAGGGATGCTCTTCCAACTCAAGGAACAGTGTGCGGCATTCCTTTGCTGCCTTCCACCGCATCAGGCTTACAATGGTTCACATTCTTCAATCTTACCAATTATAACGATGAAACCATCACCGTGGCTGTAAATGTAACCAATGTCTACATCGTGGCGTATCGTACCAATTCTGCATCCTACTTTTTTGAAGACACTTCCGCTGAAGCTTACGGGCTCTTATTTTCCGGTACTAAAAAGATAAATCTATCCTATTCCAGTAATTATGATAAGCTTCAAAGCGTTGTAGGCAAACAAAGAGATATGATTGAGCTTGGAATCCCGGCTTTAAGCAGTGCCATTTCAAACATGGTTTACTACAAATACCAACTTACTGCAGCAGCTCTTCTTGTGCTCATTCAGTCCACTGCAGAAGCCGCAAGGTATAAATATATTGAGCAACAAGTTTCTTCACATATTAGGGATAATTTTTATCCAAATCAAGCAGTCATAAGCTTAGAAAACAATTGGGGTGCTCTTTCCAAACAAATCAAGATAGCAAATAGTACAGGAAGCGGACAATTTGAAAGTGCTGTTGAGCTTATGACCCCTAATGGCACACGAGTTAGTGTAACCAATACTTCAGCTGGAGTTGTAAAAGGAAATATCAAACTCCTACTATACTCTAAAGTCAGTGTTGGTAGTGAAAATGATATCCCTACTACAATCTTGCACCCTGGAGCTATGGGAATGCTTCACAATCAGAATGGAAATTATGTTACAATGTAA

Protein sequence

MNKFIALSFFFLVISLGSPTANGDVSFDLSTATQSSYSSFITQLRDALPTQGTVCGIPLLPSTASGLQWFTFFNLTNYNDETITVAVNVTNVYIVAYRTNSASYFFEDTSAEAYGLLFSGTKKINLSYSSNYDKLQSVVGKQRDMIELGIPALSSAISNMVYYKYQLTAAALLVLIQSTAEAARYKYIEQQVSSHIRDNFYPNQAVISLENNWGALSKQIKIANSTGSGQFESAVELMTPNGTRVSVTNTSAGVVKGNIKLLLYSKVSVGSENDIPTTILHPGAMGMLHNQNGNYVTM

Homology

[Close]

BLAST of Tan0011175 vs. ExPASy Swiss-Prot
Match: P56626 (Type I ribosome-inactivating protein trichoanguina OS=Trichosanthes anguina OX=50544 GN=TCA PE=1 SV=2)

HSP 1 Score: 488.0 bits (1255), Expect = 7.4e-137
Identity = 253/294 (86.05%), Postives = 266/294 (90.48%), Query Frame = 0


Query: 5   IALSFFFLVISLGSPTANGDVSFDLSTATQSSYSSFITQLRDALPTQGTVCGIPLLPSTA 64
           +ALSFFFL ISLGSPTA GDVSFDLSTAT+ SYSSFITQLRDALPTQGTVCGIPLLPSTA
Sbjct: 1   MALSFFFLAISLGSPTAIGDVSFDLSTATKKSYSSFITQLRDALPTQGTVCGIPLLPSTA 60

Query: 65  SGLQWFTFFNLTNYNDETITVAVNVTNVYIVAYRTNSASYFFEDTSAEAYGLLFSGTKKI 124
           SG QWF FFNLTNYNDET+TVAVNVTNVYIVAYR ++ SYFFEDT AEA+ L+F+GTK +
Sbjct: 61  SGSQWFRFFNLTNYNDETVTVAVNVTNVYIVAYRADAVSYFFEDTPAEAFKLIFAGTKTV 120

Query: 125 NLSYSSNYDKLQSVVGKQRDMIELGIPALSSAISNMVYYKYQLTAAALLVLIQSTAEAAR 184
            L YS NYDKLQSVVGKQRDMIELGIPALSSAI+NMVYY YQ TAAALLVLIQ TAEAAR
Sbjct: 121 KLPYSGNYDKLQSVVGKQRDMIELGIPALSSAITNMVYYDYQSTAAALLVLIQCTAEAAR 180

Query: 185 YKYIEQQVSSHIRDNFYPNQAVISLENNWGALSKQIKIANSTGSGQFESAVELMTPNGTR 244
           YKYIEQQVSSHI  NFYPNQAVISLEN WGALSKQI+IAN TG GQFE+ VEL  P+GTR
Sbjct: 181 YKYIEQQVSSHISSNFYPNQAVISLENKWGALSKQIQIANRTGHGQFENPVELYNPDGTR 240

Query: 245 VSVTNTSAGVVKGNIKLLLYSKVSVGSENDIPTTILHPGAMGMLHNQNGNYVTM 299
            SVTNTSAGVVKGNIKLLLY K SVGSE DIPTTILHPGAMGMLHNQNGNYVTM
Sbjct: 241 FSVTNTSAGVVKGNIKLLLYYKASVGSEYDIPTTILHPGAMGMLHNQNGNYVTM 294

[Close]

BLAST of Tan0011175 vs. ExPASy Swiss-Prot
Match: P24817 (Ribosome-inactivating protein beta-momorcharin OS=Momordica charantia OX=3673 GN=MAP30 PE=1 SV=2)

HSP 1 Score: 276.9 bits (707), Expect = 2.6e-73
Identity = 152/280 (54.29%), Postives = 193/280 (68.93%), Query Frame = 0


Query: 1   MNKFIALSFFFLVISLGSPTANGDVSFDLSTATQSSYSSFITQLRDALPTQGTVCGIPLL 60
           M K + LSF  + I +G PTA GDV+FDLSTAT  +Y+ FI   R  LP    V  IPLL
Sbjct: 1   MVKCLLLSFLIIAIFIGVPTAKGDVNFDLSTATAKTYTKFIEDFRATLPFSHKVYDIPLL 60

Query: 61  PSTASGLQWFTFFNLTNYNDETITVAVNVTNVYIVAYRTNSASYFFEDTSAEAYGLLFSG 120
            ST S  + F   NLT+Y  ETI+VA++VTNVY+VAYRT   SYFF+++  EAY +LF G
Sbjct: 61  YSTISDSRRFILLNLTSYAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKG 120

Query: 121 TKKINLSYSSNYDKLQSVVGKQRDMIELGIPALSSAISNMVYYKYQLTAAALLVLIQSTA 180
           T+KI L Y+ NY+ LQ+   K R+ I+LG+PALSSAI+ + YY  Q   +ALLVLIQ+TA
Sbjct: 121 TRKITLPYTGNYENLQTAAHKIRENIDLGLPALSSAITTLFYYNAQSAPSALLVLIQTTA 180

Query: 181 EAARYKYIEQQVSSHIRDNFYPNQAVISLENNWGALSKQIKIANSTGSGQFESAVELMTP 240
           EAAR+KYIE+ V+ ++  NF PN A+ISLEN W ALSKQI +A + G G+F + V+L+ P
Sbjct: 181 EAARFKYIERHVAKYVATNFKPNLAIISLENQWSALSKQIFLAQNQG-GKFRNPVDLIKP 240

Query: 241 NGTRVSVTNTSAGVVKGNIKLLLYSKVSVGSENDIPTTIL 281
            G R  VTN  + VVKGNIKLLL S+ S   EN I T  L
Sbjct: 241 TGERFQVTNVDSDVVKGNIKLLLNSRASTADENFITTMTL 279

[Close]

BLAST of Tan0011175 vs. ExPASy Swiss-Prot
Match: P29339 (Ribosome-inactivating protein momordin II OS=Momordica balsamina OX=3672 PE=3 SV=1)

HSP 1 Score: 275.0 bits (702), Expect = 9.9e-73
Identity = 151/280 (53.93%), Postives = 193/280 (68.93%), Query Frame = 0


Query: 1   MNKFIALSFFFLVISLGSPTANGDVSFDLSTATQSSYSSFITQLRDALPTQGTVCGIPLL 60
           M K + LSF  + I +G PTA GDV+FDLSTAT  +Y+ FI   R  LP    V  IPLL
Sbjct: 1   MVKCLLLSFLIIAIFIGVPTAKGDVNFDLSTATAKTYTKFIEDFRATLPFSHKVYDIPLL 60

Query: 61  PSTASGLQWFTFFNLTNYNDETITVAVNVTNVYIVAYRTNSASYFFEDTSAEAYGLLFSG 120
            ST S  + F   +LT+Y  ETI+VA++VTNVY+VAYRT   SYFF+++  EAY +LF G
Sbjct: 61  YSTISDSRRFILLDLTSYAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKG 120

Query: 121 TKKINLSYSSNYDKLQSVVGKQRDMIELGIPALSSAISNMVYYKYQLTAAALLVLIQSTA 180
           T+KI L Y+ NY+ LQ+   K R+ I+LG+PALSSAI+ + YY  Q   +ALLVLIQ+TA
Sbjct: 121 TRKITLPYTGNYENLQTAAHKIRENIDLGLPALSSAITTLFYYNAQSAPSALLVLIQTTA 180

Query: 181 EAARYKYIEQQVSSHIRDNFYPNQAVISLENNWGALSKQIKIANSTGSGQFESAVELMTP 240
           EAAR+KYIE+ V+ ++  NF PN A+ISLEN W ALSKQI +A + G G+F + V+L+ P
Sbjct: 181 EAARFKYIERHVAKYVATNFKPNLAIISLENQWSALSKQIFLAQNQG-GKFRNPVDLIKP 240

Query: 241 NGTRVSVTNTSAGVVKGNIKLLLYSKVSVGSENDIPTTIL 281
            G R  VTN  + VVKGNIKLLL S+ S   EN I T  L
Sbjct: 241 TGERFQVTNVDSDVVKGNIKLLLNSRASTADENFITTMTL 279

[Close]

BLAST of Tan0011175 vs. ExPASy Swiss-Prot
Match: P09989 (Ribosome-inactivating protein alpha-trichosanthin OS=Trichosanthes kirilowii OX=3677 PE=1 SV=2)

HSP 1 Score: 252.3 bits (643), Expect = 6.9e-66
Identity = 143/280 (51.07%), Postives = 185/280 (66.07%), Query Frame = 0


Query: 1   MNKFIALSFFFLVISLGSPTANGDVSFDLSTATQSSYSSFITQLRDALPTQGTVCGIPLL 60
           M +F+ LS   L + L +P   GDVSF LS AT SSY  FI+ LR ALP +  +  IPLL
Sbjct: 1   MIRFLVLSLLILTLFLTTPAVEGDVSFRLSGATSSSYGVFISNLRKALPNERKLYDIPLL 60

Query: 61  PSTASGLQWFTFFNLTNYNDETITVAVNVTNVYIVAYRTNSASYFFEDTSA-EAYGLLF- 120
            S+  G Q +   +LTNY DETI+VA++VTNVYI+ YR    SYFF + SA EA   +F 
Sbjct: 61  RSSLPGSQRYALIHLTNYADETISVAIDVTNVYIMGYRAGDTSYFFNEASATEAAKYVFK 120

Query: 121 SGTKKINLSYSSNYDKLQSVVGKQRDMIELGIPALSSAISNMVYYKYQLTAAALLVLIQS 180
              +K+ L YS NY++LQ+  GK R+ I LG+PAL SAI+ + YY     A+AL+VLIQS
Sbjct: 121 DAMRKVTLPYSGNYERLQTAAGKIRENIPLGLPALDSAITTLFYYNANSAASALMVLIQS 180

Query: 181 TAEAARYKYIEQQVSSHIRDNFYPNQAVISLENNWGALSKQIKIANSTGSGQFESAVELM 240
           T+EAARYK+IEQQ+   +   F P+ A+ISLEN+W ALSKQI+IA ST +GQFES V L+
Sbjct: 181 TSEAARYKFIEQQIGKRVDKTFLPSLAIISLENSWSALSKQIQIA-STNNGQFESPVVLI 240

Query: 241 TPNGTRVSVTNTSAGVVKGNIKLLLYSKVSVGSENDIPTT 279
                RV++TN  AGVV  NI LLL        ++D+P T
Sbjct: 241 NAQNQRVTITNVDAGVVTSNIALLLNRNNMAAMDDDVPMT 279

[Close]

BLAST of Tan0011175 vs. ExPASy Swiss-Prot
Match: P33185 (Ribosome-inactivating protein bryodin I OS=Bryonia dioica OX=3652 PE=1 SV=3)

HSP 1 Score: 250.8 bits (639), Expect = 2.0e-65
Identity = 151/282 (53.55%), Postives = 183/282 (64.89%), Query Frame = 0


Query: 1   MNKFIALSFFFLVISLGSPTANGDVSFDLSTATQSSYSSFITQLRDALPTQGTVCGIPLL 60
           M K + L    L I L SPT  GDVSF LS AT +SY  FI  LR+ALP +  V  IPLL
Sbjct: 1   MIKLLVLWLLILTIFLKSPTVEGDVSFRLSGATTTSYGVFIKNLREALPYERKVYNIPLL 60

Query: 61  PSTASGLQWFTFFNLTNYNDETITVAVNVTNVYIVAYRTNSASYFFEDTSA-EAYGLLF- 120
            S+ SG   +T  +LTNY DETI+VAV+VTNVYI+ Y     SYFF + SA EA   +F 
Sbjct: 61  RSSISGSGRYTLLHLTNYADETISVAVDVTNVYIMGYLAGDVSYFFNEASATEAAKFVFK 120

Query: 121 SGTKKINLSYSSNYDKLQSVVGKQRDMIELGIPALSSAISNMVYYKYQLTAAALLVLIQS 180
              KK+ L YS NY++LQ+  GK R+ I LG+PAL SAI+ + YY     A+ALLVLIQS
Sbjct: 121 DAKKKVTLPYSGNYERLQTAAGKIRENIPLGLPALDSAITTLYYYTASSAASALLVLIQS 180

Query: 181 TAEAARYKYIEQQVSSHIRDNFYPNQAVISLENNWGALSKQIKIANSTGSGQFESAVELM 240
           TAE+ARYK+IEQQ+   +   F P+ A ISLENNW ALSKQI+IA ST +GQFES V L+
Sbjct: 181 TAESARYKFIEQQIGKRVDKTFLPSLATISLENNWSALSKQIQIA-STNNGQFESPVVLI 240

Query: 241 TPNGTRVSVTNTSAGVVKGNIKLLLYSKVSVGSENDIPTTIL 281
             N  RVS+TN SA VV  NI LLL          DI  T++
Sbjct: 241 DGNNQRVSITNASARVVTSNIALLLNRNNIAAIGEDISMTLI 281

[Close]

BLAST of Tan0011175 vs. NCBI nr
Match: P56626.2 (RecName: Full=Type I ribosome-inactivating protein trichoanguina; Short=RIP; AltName: Full=Trichoanguin; AltName: Full=rRNA N-glycosidase; Flags: Precursor [Trichosanthes anguina] >AAD02686.1 trichoanguin [Trichosanthes cucumerina])

HSP 1 Score: 488.0 bits (1255), Expect = 5.7e-134
Identity = 253/294 (86.05%), Postives = 266/294 (90.48%), Query Frame = 0


Query: 5   IALSFFFLVISLGSPTANGDVSFDLSTATQSSYSSFITQLRDALPTQGTVCGIPLLPSTA 64
           +ALSFFFL ISLGSPTA GDVSFDLSTAT+ SYSSFITQLRDALPTQGTVCGIPLLPSTA
Sbjct: 1   MALSFFFLAISLGSPTAIGDVSFDLSTATKKSYSSFITQLRDALPTQGTVCGIPLLPSTA 60

Query: 65  SGLQWFTFFNLTNYNDETITVAVNVTNVYIVAYRTNSASYFFEDTSAEAYGLLFSGTKKI 124
           SG QWF FFNLTNYNDET+TVAVNVTNVYIVAYR ++ SYFFEDT AEA+ L+F+GTK +
Sbjct: 61  SGSQWFRFFNLTNYNDETVTVAVNVTNVYIVAYRADAVSYFFEDTPAEAFKLIFAGTKTV 120

Query: 125 NLSYSSNYDKLQSVVGKQRDMIELGIPALSSAISNMVYYKYQLTAAALLVLIQSTAEAAR 184
            L YS NYDKLQSVVGKQRDMIELGIPALSSAI+NMVYY YQ TAAALLVLIQ TAEAAR
Sbjct: 121 KLPYSGNYDKLQSVVGKQRDMIELGIPALSSAITNMVYYDYQSTAAALLVLIQCTAEAAR 180

Query: 185 YKYIEQQVSSHIRDNFYPNQAVISLENNWGALSKQIKIANSTGSGQFESAVELMTPNGTR 244
           YKYIEQQVSSHI  NFYPNQAVISLEN WGALSKQI+IAN TG GQFE+ VEL  P+GTR
Sbjct: 181 YKYIEQQVSSHISSNFYPNQAVISLENKWGALSKQIQIANRTGHGQFENPVELYNPDGTR 240

Query: 245 VSVTNTSAGVVKGNIKLLLYSKVSVGSENDIPTTILHPGAMGMLHNQNGNYVTM 299
            SVTNTSAGVVKGNIKLLLY K SVGSE DIPTTILHPGAMGMLHNQNGNYVTM
Sbjct: 241 FSVTNTSAGVVKGNIKLLLYYKASVGSEYDIPTTILHPGAMGMLHNQNGNYVTM 294

[Close]

BLAST of Tan0011175 vs. NCBI nr
Match: XP_023005055.1 (type I ribosome-inactivating protein trichoanguina-like [Cucurbita maxima])

HSP 1 Score: 402.1 bits (1032), Expect = 4.1e-108
Identity = 207/277 (74.73%), Postives = 240/277 (86.64%), Query Frame = 0


Query: 1   MNKFIALSFFFLVISLGSPTANGDVSFDLSTATQSSYSSFITQLRDALPTQGTVCGIPLL 60
           MNK IA++ F + ISL SPT   D++FDLSTAT++SYS+FITQLR+ALPT  TVC IPLL
Sbjct: 1   MNKLIAITSFLVAISLISPTLKADITFDLSTATKASYSAFITQLRNALPTTATVCNIPLL 60

Query: 61  PSTASGLQWFTFFNLTNYNDETITVAVNVTNVYIVAYRTNSASYFFEDTSAEAYGLLFSG 120
           PSTASGL WFTFF +TNYN +TITVAVNVTNVYIVAYR +++SYFFEDT AEA  LLF  
Sbjct: 61  PSTASGLGWFTFFTITNYNYQTITVAVNVTNVYIVAYRADASSYFFEDTPAEAKQLLFPN 120

Query: 121 TKKINLSYSSNYDKLQSVVGKQRDMIELGIPALSSAISNMVYYKYQLTAAALLVLIQSTA 180
           T+K+ L ++ NYDKLQS+VGK RD+IELGIPALS+A++NMVYY  QLTAAALLVLIQS+A
Sbjct: 121 TQKVTLPFTGNYDKLQSIVGKYRDLIELGIPALSTAVTNMVYYNKQLTAAALLVLIQSSA 180

Query: 181 EAARYKYIEQQVSSHIRDNFYPNQAVISLENNWGALSKQIKIANSTGSGQFESAVELMTP 240
           EAAR+KYIEQQV+ HI D FYPNQAVISLENNWGALSKQI+IANSTG GQF+S VEL++P
Sbjct: 181 EAARFKYIEQQVAQHISDKFYPNQAVISLENNWGALSKQIQIANSTGRGQFQSPVELISP 240

Query: 241 NGTRVSVTNTSAGVVKGNIKLLLYSKVSVGSENDIPT 278
           NGTR SVT+TSAGVV+ NIKLLLY K+SV  END PT
Sbjct: 241 NGTRFSVTSTSAGVVQSNIKLLLYYKLSVADENDSPT 277

[Close]

BLAST of Tan0011175 vs. NCBI nr
Match: KAG6592904.1 (Type I ribosome-inactivating protein trichoanguina, partial [Cucurbita argyrosperma subsp. sororia] >KAG7025309.1 Type I ribosome-inactivating protein trichoanguina, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 394.0 bits (1011), Expect = 1.1e-105
Identity = 201/277 (72.56%), Postives = 240/277 (86.64%), Query Frame = 0


Query: 1   MNKFIALSFFFLVISLGSPTANGDVSFDLSTATQSSYSSFITQLRDALPTQGTVCGIPLL 60
           MNK IA++ F L ISL SPT   D++FDLSTAT++SYS+FITQLR+ALPT+ TVC IPLL
Sbjct: 1   MNKLIAITSFLLAISLISPTLKADIAFDLSTATKASYSAFITQLRNALPTKATVCNIPLL 60

Query: 61  PSTASGLQWFTFFNLTNYNDETITVAVNVTNVYIVAYRTNSASYFFEDTSAEAYGLLFSG 120
           PSTA+GL WFTFF +TNYN +T+ VAVNV+NVYIVAYR ++ SYFFEDT A+A  LLF+G
Sbjct: 61  PSTATGLGWFTFFTITNYNYQTVEVAVNVSNVYIVAYRADANSYFFEDTPAQAKQLLFAG 120

Query: 121 TKKINLSYSSNYDKLQSVVGKQRDMIELGIPALSSAISNMVYYKYQLTAAALLVLIQSTA 180
           T+K+ L ++ NYDKLQS+VGK RDM+ELGIPALS+A++NMVYY  Q TAAALLVLIQSTA
Sbjct: 121 TQKVTLPFTGNYDKLQSIVGKYRDMVELGIPALSTAVTNMVYYNKQPTAAALLVLIQSTA 180

Query: 181 EAARYKYIEQQVSSHIRDNFYPNQAVISLENNWGALSKQIKIANSTGSGQFESAVELMTP 240
           EAAR+KYIEQQV+ +I   FYPNQAVISLENNWGALSKQI+IANSTGSGQF+S VEL++P
Sbjct: 181 EAARFKYIEQQVAQYISGKFYPNQAVISLENNWGALSKQIQIANSTGSGQFQSPVELISP 240

Query: 241 NGTRVSVTNTSAGVVKGNIKLLLYSKVSVGSENDIPT 278
           NGTR  VT+TS+GVV+GNIKLLLY K+SV  +ND PT
Sbjct: 241 NGTRFRVTSTSSGVVQGNIKLLLYYKLSVADDNDSPT 277

[Close]

BLAST of Tan0011175 vs. NCBI nr
Match: XP_023513430.1 (type I ribosome-inactivating protein trichoanguina-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 392.9 bits (1008), Expect = 2.5e-105
Identity = 202/277 (72.92%), Postives = 236/277 (85.20%), Query Frame = 0


Query: 1   MNKFIALSFFFLVISLGSPTANGDVSFDLSTATQSSYSSFITQLRDALPTQGTVCGIPLL 60
           MNK IA+S F L ISL SPT   D++ DLSTAT++SYS+FIT +R+ALPT+ TVC IPLL
Sbjct: 1   MNKLIAISSFLLAISLISPTLKADIALDLSTATKASYSAFITHIRNALPTKATVCNIPLL 60

Query: 61  PSTASGLQWFTFFNLTNYNDETITVAVNVTNVYIVAYRTNSASYFFEDTSAEAYGLLFSG 120
           PST +GL  FTFF +TNYN +T+ VAVNVTNVYIVAYR  ++SYFFEDT A+A  LLF+G
Sbjct: 61  PSTPTGLASFTFFTITNYNYQTVEVAVNVTNVYIVAYRAGASSYFFEDTPAQAKQLLFAG 120

Query: 121 TKKINLSYSSNYDKLQSVVGKQRDMIELGIPALSSAISNMVYYKYQLTAAALLVLIQSTA 180
           T+K+ L ++ NYDKLQS+VGK RDM+ELGIPALS+A++NMVYY  QLTAAALLVLIQSTA
Sbjct: 121 TQKVTLPFTGNYDKLQSIVGKYRDMVELGIPALSTAVTNMVYYNKQLTAAALLVLIQSTA 180

Query: 181 EAARYKYIEQQVSSHIRDNFYPNQAVISLENNWGALSKQIKIANSTGSGQFESAVELMTP 240
           EAAR+KYIEQQV+ HI D FYPNQAVISLENNWGALSKQI+IANSTG GQF+S VEL+ P
Sbjct: 181 EAARFKYIEQQVAQHISDKFYPNQAVISLENNWGALSKQIQIANSTGRGQFQSPVELIGP 240

Query: 241 NGTRVSVTNTSAGVVKGNIKLLLYSKVSVGSENDIPT 278
            GTR SVTNTSAGVV+GNIKLLLY K+SV  +ND PT
Sbjct: 241 TGTRFSVTNTSAGVVQGNIKLLLYYKLSVADDNDSPT 277

[Close]

BLAST of Tan0011175 vs. NCBI nr
Match: XP_022156709.1 (type I ribosome-inactivating protein trichoanguina-like [Momordica charantia])

HSP 1 Score: 391.3 bits (1004), Expect = 7.2e-105
Identity = 208/286 (72.73%), Postives = 241/286 (84.27%), Query Frame = 0


Query: 1   MNKFIALSFFFLVISLGSPTANGDVSFDLSTATQSSYSSFITQLRDALPTQGTVCGIPLL 60
           M K IALSFF L I LG PTA GDVSFDLSTAT++SY+SFI  LR ALPT+GTVC IP+L
Sbjct: 1   MMKSIALSFFILTIFLGPPTAKGDVSFDLSTATKNSYTSFIRDLRSALPTRGTVCNIPVL 60

Query: 61  PSTASGLQWFTFFNLTNYNDETITVAVNVTNVYIVAYRTNSASYFFEDTSAEAYGLLFSG 120
           PSTASGLQWF FF+LTNYN +TITVAVNVTNVYI AY T + SYFFEDTSAEA+ LLF G
Sbjct: 61  PSTASGLQWFRFFSLTNYNYQTITVAVNVTNVYIAAYSTGAVSYFFEDTSAEAFRLLFVG 120

Query: 121 TKKINLSYSSNYDKLQSVVGKQRDMIELGIPALSSAISNMVY-YKYQLTAAALLVLIQST 180
           T+K+ L YS NYD+LQS+VGK RD+IELGIP LS+AI+N+ Y   Y+LTA ALLVLIQST
Sbjct: 121 TQKVTLPYSGNYDRLQSIVGKNRDLIELGIPTLSNAITNLFYNNNYKLTATALLVLIQST 180

Query: 181 AEAARYKYIEQQVSSHIRDNFYPNQAVISLENNWGALSKQIKIANSTGSGQFESAVELMT 240
           AEAAR+KYIEQ+VS H+  NF+PNQA+ISLEN+WGALSKQI+IANS G+GQF+ AVEL+T
Sbjct: 181 AEAARFKYIEQEVSKHVSSNFFPNQAIISLENSWGALSKQIQIANSKGTGQFDKAVELIT 240

Query: 241 PNGTRVSVTNTSAGVVKGNIKLLLYSKVSVGSENDIPTTILHPGAM 286
            +G RVSVTNTS GVVK NIKLLLY KV+  ++N IP TIL  GA+
Sbjct: 241 IDGKRVSVTNTSTGVVKSNIKLLLYYKVNGANDNYIP-TILPLGAI 285

[Close]

BLAST of Tan0011175 vs. ExPASy TrEMBL
Match: A0A6J1KWC4 (rRNA N-glycosidase OS=Cucurbita maxima OX=3661 GN=LOC111498162 PE=3 SV=1)

HSP 1 Score: 402.1 bits (1032), Expect = 2.0e-108
Identity = 207/277 (74.73%), Postives = 240/277 (86.64%), Query Frame = 0


Query: 1   MNKFIALSFFFLVISLGSPTANGDVSFDLSTATQSSYSSFITQLRDALPTQGTVCGIPLL 60
           MNK IA++ F + ISL SPT   D++FDLSTAT++SYS+FITQLR+ALPT  TVC IPLL
Sbjct: 1   MNKLIAITSFLVAISLISPTLKADITFDLSTATKASYSAFITQLRNALPTTATVCNIPLL 60

Query: 61  PSTASGLQWFTFFNLTNYNDETITVAVNVTNVYIVAYRTNSASYFFEDTSAEAYGLLFSG 120
           PSTASGL WFTFF +TNYN +TITVAVNVTNVYIVAYR +++SYFFEDT AEA  LLF  
Sbjct: 61  PSTASGLGWFTFFTITNYNYQTITVAVNVTNVYIVAYRADASSYFFEDTPAEAKQLLFPN 120

Query: 121 TKKINLSYSSNYDKLQSVVGKQRDMIELGIPALSSAISNMVYYKYQLTAAALLVLIQSTA 180
           T+K+ L ++ NYDKLQS+VGK RD+IELGIPALS+A++NMVYY  QLTAAALLVLIQS+A
Sbjct: 121 TQKVTLPFTGNYDKLQSIVGKYRDLIELGIPALSTAVTNMVYYNKQLTAAALLVLIQSSA 180

Query: 181 EAARYKYIEQQVSSHIRDNFYPNQAVISLENNWGALSKQIKIANSTGSGQFESAVELMTP 240
           EAAR+KYIEQQV+ HI D FYPNQAVISLENNWGALSKQI+IANSTG GQF+S VEL++P
Sbjct: 181 EAARFKYIEQQVAQHISDKFYPNQAVISLENNWGALSKQIQIANSTGRGQFQSPVELISP 240

Query: 241 NGTRVSVTNTSAGVVKGNIKLLLYSKVSVGSENDIPT 278
           NGTR SVT+TSAGVV+ NIKLLLY K+SV  END PT
Sbjct: 241 NGTRFSVTSTSAGVVQSNIKLLLYYKLSVADENDSPT 277

[Close]

BLAST of Tan0011175 vs. ExPASy TrEMBL
Match: A0A6J1DUC7 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111023554 PE=3 SV=1)

HSP 1 Score: 391.3 bits (1004), Expect = 3.5e-105
Identity = 208/286 (72.73%), Postives = 241/286 (84.27%), Query Frame = 0


Query: 1   MNKFIALSFFFLVISLGSPTANGDVSFDLSTATQSSYSSFITQLRDALPTQGTVCGIPLL 60
           M K IALSFF L I LG PTA GDVSFDLSTAT++SY+SFI  LR ALPT+GTVC IP+L
Sbjct: 1   MMKSIALSFFILTIFLGPPTAKGDVSFDLSTATKNSYTSFIRDLRSALPTRGTVCNIPVL 60

Query: 61  PSTASGLQWFTFFNLTNYNDETITVAVNVTNVYIVAYRTNSASYFFEDTSAEAYGLLFSG 120
           PSTASGLQWF FF+LTNYN +TITVAVNVTNVYI AY T + SYFFEDTSAEA+ LLF G
Sbjct: 61  PSTASGLQWFRFFSLTNYNYQTITVAVNVTNVYIAAYSTGAVSYFFEDTSAEAFRLLFVG 120

Query: 121 TKKINLSYSSNYDKLQSVVGKQRDMIELGIPALSSAISNMVY-YKYQLTAAALLVLIQST 180
           T+K+ L YS NYD+LQS+VGK RD+IELGIP LS+AI+N+ Y   Y+LTA ALLVLIQST
Sbjct: 121 TQKVTLPYSGNYDRLQSIVGKNRDLIELGIPTLSNAITNLFYNNNYKLTATALLVLIQST 180

Query: 181 AEAARYKYIEQQVSSHIRDNFYPNQAVISLENNWGALSKQIKIANSTGSGQFESAVELMT 240
           AEAAR+KYIEQ+VS H+  NF+PNQA+ISLEN+WGALSKQI+IANS G+GQF+ AVEL+T
Sbjct: 181 AEAARFKYIEQEVSKHVSSNFFPNQAIISLENSWGALSKQIQIANSKGTGQFDKAVELIT 240

Query: 241 PNGTRVSVTNTSAGVVKGNIKLLLYSKVSVGSENDIPTTILHPGAM 286
            +G RVSVTNTS GVVK NIKLLLY KV+  ++N IP TIL  GA+
Sbjct: 241 IDGKRVSVTNTSTGVVKSNIKLLLYYKVNGANDNYIP-TILPLGAI 285

[Close]

BLAST of Tan0011175 vs. ExPASy TrEMBL
Match: A0A6J1H951 (rRNA N-glycosidase OS=Cucurbita moschata OX=3662 GN=LOC111461188 PE=3 SV=1)

HSP 1 Score: 386.7 bits (992), Expect = 8.6e-104
Identity = 201/277 (72.56%), Postives = 236/277 (85.20%), Query Frame = 0


Query: 1   MNKFIALSFFFLVISLGSPTANGDVSFDLSTATQSSYSSFITQLRDALPTQGTVCGIPLL 60
           MNK IA++ F L ISL SPT   D++ DLSTAT+++YS+FITQLR+ALPT+ TVC IPLL
Sbjct: 1   MNKLIAITSFLLAISLISPTLKADIALDLSTATKATYSAFITQLRNALPTKATVCNIPLL 60

Query: 61  PSTASGLQWFTFFNLTNYNDETITVAVNVTNVYIVAYRTNSASYFFEDTSAEAYGLLFSG 120
           PSTA+GL  FTFF +TNYN ET+ VAVNV+NVYIVAYR N+ SYFFEDTSA+A  LLF  
Sbjct: 61  PSTATGLGSFTFFTITNYNYETVEVAVNVSNVYIVAYRANAKSYFFEDTSAQARQLLFQN 120

Query: 121 TKKINLSYSSNYDKLQSVVGKQRDMIELGIPALSSAISNMVYYKYQLTAAALLVLIQSTA 180
           T+KI L ++ NYD+LQ VVGK RD IELGIPALS+A++NMVYY  QLTAAALLVLIQSTA
Sbjct: 121 TQKITLPFTGNYDRLQGVVGKYRDSIELGIPALSTAVTNMVYYNKQLTAAALLVLIQSTA 180

Query: 181 EAARYKYIEQQVSSHIRDNFYPNQAVISLENNWGALSKQIKIANSTGSGQFESAVELMTP 240
           EAAR+KYIEQQV+ +I   FYPNQAVISLENNWGALSKQI+IANSTGSGQF+S VEL++P
Sbjct: 181 EAARFKYIEQQVAQYISGKFYPNQAVISLENNWGALSKQIQIANSTGSGQFQSPVELISP 240

Query: 241 NGTRVSVTNTSAGVVKGNIKLLLYSKVSVGSENDIPT 278
           NGTR  VT+TS+GVV+GNIKLLLY K+SV  +ND PT
Sbjct: 241 NGTRFRVTSTSSGVVQGNIKLLLYYKLSVADDNDSPT 277

[Close]

BLAST of Tan0011175 vs. ExPASy TrEMBL
Match: A0A6J1DR40 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111023561 PE=3 SV=1)

HSP 1 Score: 335.5 bits (859), Expect = 2.3e-88
Identity = 182/298 (61.07%), Postives = 226/298 (75.84%), Query Frame = 0


Query: 1   MNKFIALSFFFLVISLGSPTANGDVSFDLSTATQSSYSSFITQLRDALPTQGTVCGIPLL 60
           M K   LS   L I LG  +A GDVSF LS A + SY++FI QLRDA+PT  TVC IP+L
Sbjct: 1   MRKLTTLS-LLLAILLGPLSAEGDVSFQLSGANKKSYTNFIKQLRDAVPTTTTVCKIPVL 60

Query: 61  PSTASGLQWFTFFNLTNYNDETITVAVNVTNVYIVAYRTNSASYFFEDTSAEAYGLLFSG 120
           PSTASG + FTF +LTNY D+T+ VA+N+TNVYIVAY  +  SYFF+D S EAY +LF G
Sbjct: 61  PSTASGPKSFTFVHLTNYADQTVVVAINITNVYIVAYHIDHVSYFFDDASPEAYKVLFEG 120

Query: 121 TKKINLSYSSNYDKLQSVVGKQRDMIELGIPALSSAISNMVYYKYQLTAAALLVLIQSTA 180
           T +  L YSSNYDKLQS+VGK+RD+IELG+PALSSAI+N+VYY+Y+ TAAALLVLIQS A
Sbjct: 121 TDRKTLPYSSNYDKLQSIVGKKRDLIELGLPALSSAITNLVYYEYKSTAAALLVLIQSVA 180

Query: 181 EAARYKYIEQQVSSHIRDNFYPNQAVISLENNWGALSKQIKIANSTGSGQFESAVELMTP 240
           EAARYKYIEQ++S+H+ D F P+QA+ISLENNWGALSKQI+IA + G+GQFE  VEL+ P
Sbjct: 181 EAARYKYIEQEISTHVDDKFKPDQAIISLENNWGALSKQIQIA-AKGNGQFEKPVELINP 240

Query: 241 NGTRVSVTNTSAGVVKGNIKLLLYSKVSVGSENDIPTTILHPGAMGMLHNQNGNYVTM 299
           N T  SVTN S+ VV  NIKLLLY K++  + +D   T+  P      +N   N++T+
Sbjct: 241 NNTPFSVTNVSSLVVTSNIKLLLYFKLAAANHDDQDKTVGKP-----FNNGGENFITV 291

[Close]

BLAST of Tan0011175 vs. ExPASy TrEMBL
Match: A0A6J1DRC7 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111023553 PE=3 SV=1)

HSP 1 Score: 277.3 bits (708), Expect = 7.4e-71
Identity = 152/280 (54.29%), Postives = 193/280 (68.93%), Query Frame = 0


Query: 1   MNKFIALSFFFLVISLGSPTANGDVSFDLSTATQSSYSSFITQLRDALPTQGTVCGIPLL 60
           M K + LSF  + I +G PTA GDV+FDLSTAT  +Y+ FI   R  LP    V  IPLL
Sbjct: 1   MMKCLLLSFLIIAIFIGVPTAKGDVNFDLSTATAKTYTKFIEDFRATLPFSHKVYDIPLL 60

Query: 61  PSTASGLQWFTFFNLTNYNDETITVAVNVTNVYIVAYRTNSASYFFEDTSAEAYGLLFSG 120
            ST S  + F   NLT+Y  ETI+VA++VTNVY+VAYRT   SYFF+++  EAY +LF G
Sbjct: 61  YSTISDSRRFILLNLTSYAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKG 120

Query: 121 TKKINLSYSSNYDKLQSVVGKQRDMIELGIPALSSAISNMVYYKYQLTAAALLVLIQSTA 180
           T+KI L Y+ NY+ LQ+   K R+ I+LG+PALSSAI+ + YY  Q   +ALLVLIQ+TA
Sbjct: 121 TRKITLPYTGNYENLQTAAHKIRENIDLGLPALSSAITTLFYYNAQSAPSALLVLIQTTA 180

Query: 181 EAARYKYIEQQVSSHIRDNFYPNQAVISLENNWGALSKQIKIANSTGSGQFESAVELMTP 240
           EAAR+KYIE+ V+ ++  NF PN A+ISLEN W ALSKQI +A + G G+F + V+L+ P
Sbjct: 181 EAARFKYIERHVAKYVATNFKPNLAIISLENQWSALSKQIFLAQNQG-GKFRNPVDLIKP 240

Query: 241 NGTRVSVTNTSAGVVKGNIKLLLYSKVSVGSENDIPTTIL 281
            G R  VTN  + VVKGNIKLLL S+ S   EN I T  L
Sbjct: 241 TGERFQVTNVDSDVVKGNIKLLLNSRASTADENFITTMTL 279

The following BLAST results are available for this feature:

BLAST of Tan0011175 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P56626	7.4e-137	86.05	Type I ribosome-inactivating protein trichoanguina OS=Trichosanthes anguina OX=5...	[more]
P24817	2.6e-73	54.29	Ribosome-inactivating protein beta-momorcharin OS=Momordica charantia OX=3673 GN...	[more]
P29339	9.9e-73	53.93	Ribosome-inactivating protein momordin II OS=Momordica balsamina OX=3672 PE=3 SV...	[more]
P09989	6.9e-66	51.07	Ribosome-inactivating protein alpha-trichosanthin OS=Trichosanthes kirilowii OX=...	[more]
P33185	2.0e-65	53.55	Ribosome-inactivating protein bryodin I OS=Bryonia dioica OX=3652 PE=1 SV=3	[more]

BLAST of Tan0011175 vs. NCBI nr


Match Name	E-value	Identity	Description
P56626.2	5.7e-134	86.05	RecName: Full=Type I ribosome-inactivating protein trichoanguina; Short=RIP; Alt...	[more]
XP_023005055.1	4.1e-108	74.73	type I ribosome-inactivating protein trichoanguina-like [Cucurbita maxima]	[more]
KAG6592904.1	1.1e-105	72.56	Type I ribosome-inactivating protein trichoanguina, partial [Cucurbita argyrospe...	[more]
XP_023513430.1	2.5e-105	72.92	type I ribosome-inactivating protein trichoanguina-like [Cucurbita pepo subsp. p...	[more]
XP_022156709.1	7.2e-105	72.73	type I ribosome-inactivating protein trichoanguina-like [Momordica charantia]	[more]

BLAST of Tan0011175 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KWC4	2.0e-108	74.73	rRNA N-glycosidase OS=Cucurbita maxima OX=3661 GN=LOC111498162 PE=3 SV=1	[more]
A0A6J1DUC7	3.5e-105	72.73	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111023554 PE=3 SV=1	[more]
A0A6J1H951	8.6e-104	72.56	rRNA N-glycosidase OS=Cucurbita moschata OX=3662 GN=LOC111461188 PE=3 SV=1	[more]
A0A6J1DR40	2.3e-88	61.07	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111023561 PE=3 SV=1	[more]
A0A6J1DRC7	7.4e-71	54.29	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111023553 PE=3 SV=1	[more]

BLAST of Tan0011175 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR017989	Ribosome-inactivating protein type 1/2	PRINTS	PR00396	SHIGARICIN	coord: 172..192 score: 63.91 coord: 204..221 score: 49.12 coord: 82..97 score: 52.63 coord: 143..157 score: 51.75 coord: 35..48 score: 45.68
IPR001574	Ribosome-inactivating protein	PFAM	PF00161	RIP	coord: 33..220 e-value: 2.6E-32 score: 112.1
IPR001574	Ribosome-inactivating protein	PANTHER	PTHR33453	FAMILY NOT NAMED	coord: 24..268
IPR016139	Ribosome-inactivating protein, subdomain 2	GENE3D	4.10.470.10	Ricin (A Subunit), domain 2	coord: 184..270 e-value: 2.4E-28 score: 99.8
IPR016138	Ribosome-inactivating protein, subdomain 1	GENE3D	3.40.420.10	Ricin (A subunit), domain 1	coord: 24..183 e-value: 5.5E-44 score: 151.7
None	No IPR available	PIRSR	PIRSR001924-1	PIRSR001924-1	coord: 7..218 e-value: 7.2E-6 score: 23.3
None	No IPR available	PANTHER	PTHR33453:SF14	RRNA N-GLYCOSIDASE	coord: 24..268
IPR017988	Ribosome-inactivating protein conserved site	PROSITE	PS00275	SHIGA_RICIN	coord: 176..192
IPR036041	Ribosome-inactivating protein superfamily	SUPERFAMILY	56371	Ribosome inactivating proteins (RIP)	coord: 24..271

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Tan0011175.1	Tan0011175.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006952	defense response
biological_process	GO:0035821	modulation of process of other organism
biological_process	GO:0017148	negative regulation of translation
molecular_function	GO:0030598	rRNA N-glycosylase activity
molecular_function	GO:0090729	toxin activity