Tan0010525 (gene) Snake gourd v1

Overview


Name	Tan0010525
Type	gene
Organism	Trichosanthes anguina (Snake gourd v1)
Description	Calcium-binding EF-hand family protein, putative
Location	LG01: 113909557 .. 113910656 (+)
RNA-Seq Expression	Tan0010525
Synteny	Tan0010525

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GGTGTATATATGTTTAATCCTCATCCTTGGAGAAAAGACTTATTCCCATGGCGGCATTGACCAATTTTAACTTTAACCTCAAACAGCAGGCACCATTAATCAACGCAAACGACAGTTCCCAAATTGGACGACACTGAAGGACCGAATTCGATTCCATTTTTTTGCCGCAGCGATCGAATCAGAAAGCCGTAACAATCCCATTGGAGAAGATAACTCCGCATCTTAATTCAAAATCTAATCTGCTCGCGCGCCGTCGATGGTGGCTCGCGATGAATCCTCAATATTTCTATATAAATTCCCCTTTCCTCCACTTACAAATACAATCAATTACAGAACGATGTCGTCGTACGGTCGAAAATCGAAGTCGAAGGCGGCGGCCGTTCCGTCGGAGAACGGCTACTCGGCGAAGGAACTGAGAGAGATCTTTAGCCAGATGGATACGAATGAAGACGGGGGATTGGGATTGATGGAGATCAAGGACGCATTGAAGCGACTGGACGCTGAATTACCGGTTCATCTTGCCTTCAAGGCGCTCTGCCACGCCGATCGAAACAGAGATGGAAAGCTTGGGGAGGATGAGATCGAAAAGTTCGTCAAGTATTTGATGAGCCAAGGTTATGTTGTGTGATTTAACTAATCCGACGAAGCCTCTTACTATTGCTTGTTCATCGTCGGTTTCTAAAAACTTATCCAATAAATGGCGGATGCGTCTTATATATTGTTCATCAAGTTATTACAGAGATTTGTATTGTATTGTATTGTATATATTGTTTCTTTTTAATGCCCATCTTTAACATCAGTTTTTTTTTTTTTTTTAGTGCTGGCACCTATAGGGTTGTAAATTTGTTTTTTTAAGGTTGAGAGAAATCTCCCACTCATGTATTTGTTGAATTAAAAAAAAATTATTTTGTCCTAAAAATTGAATATTGGATATTTTTGCTAATTCATTTTCAAAGATAAAAGGTTCTCAATTTTTTTTAATGGTTCACTTGAATTTAGCTCAAATAATTAGGCCTATATTCTCGATCCTAGATTAGAAATTTGAATCGCTATCCTAAATGTTGTTGAACTAAAACTATGTTTGAATGAAATTGTAATTC

mRNA sequence

GGTGTATATATGTTTAATCCTCATCCTTGGAGAAAAGACTTATTCCCATGGCGGCATTGACCAATTTTAACTTTAACCTCAAACAGCAGGCACCATTAATCAACGCAAACGACAGTTCCCAAATTGGACGACACTGAAGGACCGAATTCGATTCCATTTTTTTGCCGCAGCGATCGAATCAGAAAGCCGTAACAATCCCATTGGAGAAGATAACTCCGCATCTTAATTCAAAATCTAATCTGCTCGCGCGCCGTCGATGGTGGCTCGCGATGAATCCTCAATATTTCTATATAAATTCCCCTTTCCTCCACTTACAAATACAATCAATTACAGAACGATGTCGTCGTACGGTCGAAAATCGAAGTCGAAGGCGGCGGCCGTTCCGTCGGAGAACGGCTACTCGGCGAAGGAACTGAGAGAGATCTTTAGCCAGATGGATACGAATGAAGACGGGGGATTGGGATTGATGGAGATCAAGGACGCATTGAAGCGACTGGACGCTGAATTACCGGTTCATCTTGCCTTCAAGGCGCTCTGCCACGCCGATCGAAACAGAGATGGAAAGCTTGGGGAGGATGAGATCGAAAAGTTCGTCAAGTATTTGATGAGCCAAGGTTATGTTGTGTGATTTAACTAATCCGACGAAGCCTCTTACTATTGCTTGTTCATCGTCGGTTTCTAAAAACTTATCCAATAAATGGCGGATGCGTCTTATATATTGTTCATCAAGTTATTACAGAGATTTGTATTGTATTGTATTGTATATATTGTTTCTTTTTAATGCCCATCTTTAACATCAGTTTTTTTTTTTTTTTTAGTGCTGGCACCTATAGGGTTGTAAATTTGTTTTTTTAAGGTTGAGAGAAATCTCCCACTCATGTATTTGTTGAATTAAAAAAAAATTATTTTGTCCTAAAAATTGAATATTGGATATTTTTGCTAATTCATTTTCAAAGATAAAAGGTTCTCAATTTTTTTTAATGGTTCACTTGAATTTAGCTCAAATAATTAGGCCTATATTCTCGATCCTAGATTAGAAATTTGAATCGCTATCCTAAATGTTGTTGAACTAAAACTATGTTTGAATGAAATTGTAATTC

Coding sequence (CDS)

ATGGTGGCTCGCGATGAATCCTCAATATTTCTATATAAATTCCCCTTTCCTCCACTTACAAATACAATCAATTACAGAACGATGTCGTCGTACGGTCGAAAATCGAAGTCGAAGGCGGCGGCCGTTCCGTCGGAGAACGGCTACTCGGCGAAGGAACTGAGAGAGATCTTTAGCCAGATGGATACGAATGAAGACGGGGGATTGGGATTGATGGAGATCAAGGACGCATTGAAGCGACTGGACGCTGAATTACCGGTTCATCTTGCCTTCAAGGCGCTCTGCCACGCCGATCGAAACAGAGATGGAAAGCTTGGGGAGGATGAGATCGAAAAGTTCGTCAAGTATTTGATGAGCCAAGGTTATGTTGTGTGA

Protein sequence

MVARDESSIFLYKFPFPPLTNTINYRTMSSYGRKSKSKAAAVPSENGYSAKELREIFSQMDTNEDGGLGLMEIKDALKRLDAELPVHLAFKALCHADRNRDGKLGEDEIEKFVKYLMSQGYVV

Homology

[Close]

BLAST of Tan0010525 vs. ExPASy Swiss-Prot
Match: Q32NH8 (1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-4 OS=Xenopus laevis OX=8355 GN=plcd4 PE=2 SV=1)

HSP 1 Score: 44.7 bits (104), Expect = 9.0e-04
Identity = 21/67 (31.34%), Postives = 36/67 (53.73%), Query Frame = 0


Query: 57  FSQMDTNEDGGLGLMEIKDALKRLDAELPVHLAFKALCHADRNRDGKLGEDEIEKFVKYL 116
           F + D N+DG +   E++D LK ++ ++  H AF+    AD++  G L  +E   F K L
Sbjct: 143 FQKADKNKDGRMNFKEVQDLLKMMNVDMSEHHAFRLFQMADKSESGTLEGEEFVLFYKAL 202

Query: 117 MSQGYVV 124
             +  V+
Sbjct: 203 TQRDEVL 209

[Close]

BLAST of Tan0010525 vs. NCBI nr
Match: KAG6608479.1 (hypothetical protein SDJN03_01821, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 168.7 bits (426), Expect = 3.1e-38
Identity = 82/105 (78.10%), Postives = 94/105 (89.52%), Query Frame = 0


Query: 19  LTNTINYRTMSSYGRKSKSKAAAVPSENGYSAKELREIFSQMDTNEDGGLGLMEIKDALK 78
           + N ++Y+TMSSYGR  KSKA A  SE+GYSAKELREIF+QMDTNEDGGLGL EIK+ALK
Sbjct: 32  IRNPVDYKTMSSYGR--KSKAVAAQSESGYSAKELREIFNQMDTNEDGGLGLFEIKEALK 91

Query: 79  RLDAELPVHLAFKALCHADRNRDGKLGEDEIEKFVKYLMSQGYVV 124
           +LDAE PVHLAFKALC +DRNRDGKLG DEI+KF+KYLMS+GYVV
Sbjct: 92  QLDAEFPVHLAFKALCQSDRNRDGKLGRDEIDKFIKYLMSEGYVV 134

[Close]

BLAST of Tan0010525 vs. NCBI nr
Match: KAG7037807.1 (hypothetical protein SDJN02_01438, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 168.3 bits (425), Expect = 4.1e-38
Identity = 82/103 (79.61%), Postives = 93/103 (90.29%), Query Frame = 0


Query: 21  NTINYRTMSSYGRKSKSKAAAVPSENGYSAKELREIFSQMDTNEDGGLGLMEIKDALKRL 80
           N ++Y+TMSSYGR  KSKA A  SE+GYSAKELREIF+QMDTNEDGGLGL EIK+ALK+L
Sbjct: 34  NPVDYKTMSSYGR--KSKAVAAQSESGYSAKELREIFNQMDTNEDGGLGLFEIKEALKQL 93

Query: 81  DAELPVHLAFKALCHADRNRDGKLGEDEIEKFVKYLMSQGYVV 124
           DAE PVHLAFKALC +DRNRDGKLG DEI+KF+KYLMS+GYVV
Sbjct: 94  DAEFPVHLAFKALCQSDRNRDGKLGRDEIDKFIKYLMSEGYVV 134

[Close]

BLAST of Tan0010525 vs. NCBI nr
Match: KGN55187.1 (hypothetical protein Csa_012728 [Cucumis sativus])

HSP 1 Score: 147.9 bits (372), Expect = 5.7e-32
Identity = 70/95 (73.68%), Postives = 85/95 (89.47%), Query Frame = 0


Query: 29  SSYGRKSKSKAAAVPSENGYSAKELREIFSQMDTNEDGGLGLMEIKDALKRLDAELPVHL 88
           S +  K+KS +  V SE+ YSAKELR+IF+QMDTNEDGGLGL EIK+ALK+LDAE+PVHL
Sbjct: 3   SMFRPKTKSNSPPVSSESAYSAKELRQIFNQMDTNEDGGLGLFEIKNALKKLDAEMPVHL 62

Query: 89  AFKALCHADRNRDGKLGEDEIEKFVKYLMSQGYVV 124
           A+ ALCHADRNRDGKLGE+EI++FVKY+MS+GYVV
Sbjct: 63  AYSALCHADRNRDGKLGEEEIDQFVKYMMSKGYVV 97

[Close]

BLAST of Tan0010525 vs. NCBI nr
Match: KAA0064378.1 (putative calcium-binding protein CML10 [Cucumis melo var. makuwa] >TYK20209.1 putative calcium-binding protein CML10 [Cucumis melo var. makuwa])

HSP 1 Score: 144.8 bits (364), Expect = 4.9e-31
Identity = 68/88 (77.27%), Postives = 82/88 (93.18%), Query Frame = 0


Query: 36  KSKAAAVPSENGYSAKELREIFSQMDTNEDGGLGLMEIKDALKRLDAELPVHLAFKALCH 95
           KS + AV SE+ YSAKELR+IF+QMDTNEDGGLGL EIK+ALK+LDAE+PVHLA+ ALC+
Sbjct: 2   KSNSRAVSSESAYSAKELRQIFNQMDTNEDGGLGLFEIKNALKKLDAEMPVHLAYSALCY 61

Query: 96  ADRNRDGKLGEDEIEKFVKYLMSQGYVV 124
           ADRNRDGKLGE+EI++FVKY+MS+GYVV
Sbjct: 62  ADRNRDGKLGEEEIDQFVKYMMSKGYVV 89

[Close]

BLAST of Tan0010525 vs. NCBI nr
Match: KAG7024250.1 (hypothetical protein SDJN02_13064, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 133.7 bits (335), Expect = 1.1e-27
Identity = 61/80 (76.25%), Postives = 74/80 (92.50%), Query Frame = 0


Query: 44  SENGYSAKELREIFSQMDTNEDGGLGLMEIKDALKRLDAELPVHLAFKALCHADRNRDGK 103
           S++ YS KEL+EIF QMDTNEDGGLGL EIK+ALK+LDAE P+HLA+KALCHADRN DGK
Sbjct: 10  SKSAYSTKELKEIFHQMDTNEDGGLGLFEIKNALKQLDAEFPIHLAYKALCHADRNIDGK 69

Query: 104 LGEDEIEKFVKYLMSQGYVV 124
           LGE+EI++FVKY++S+GYVV
Sbjct: 70  LGEEEIDEFVKYMLSEGYVV 89

[Close]

BLAST of Tan0010525 vs. ExPASy TrEMBL
Match: A0A0A0L3J7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G639750 PE=4 SV=1)

HSP 1 Score: 147.9 bits (372), Expect = 2.8e-32
Identity = 70/95 (73.68%), Postives = 85/95 (89.47%), Query Frame = 0


Query: 29  SSYGRKSKSKAAAVPSENGYSAKELREIFSQMDTNEDGGLGLMEIKDALKRLDAELPVHL 88
           S +  K+KS +  V SE+ YSAKELR+IF+QMDTNEDGGLGL EIK+ALK+LDAE+PVHL
Sbjct: 3   SMFRPKTKSNSPPVSSESAYSAKELRQIFNQMDTNEDGGLGLFEIKNALKKLDAEMPVHL 62

Query: 89  AFKALCHADRNRDGKLGEDEIEKFVKYLMSQGYVV 124
           A+ ALCHADRNRDGKLGE+EI++FVKY+MS+GYVV
Sbjct: 63  AYSALCHADRNRDGKLGEEEIDQFVKYMMSKGYVV 97

[Close]

BLAST of Tan0010525 vs. ExPASy TrEMBL
Match: A0A5D3D9N2 (Putative calcium-binding protein CML10 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G003460 PE=4 SV=1)

HSP 1 Score: 144.8 bits (364), Expect = 2.4e-31
Identity = 68/88 (77.27%), Postives = 82/88 (93.18%), Query Frame = 0


Query: 36  KSKAAAVPSENGYSAKELREIFSQMDTNEDGGLGLMEIKDALKRLDAELPVHLAFKALCH 95
           KS + AV SE+ YSAKELR+IF+QMDTNEDGGLGL EIK+ALK+LDAE+PVHLA+ ALC+
Sbjct: 2   KSNSRAVSSESAYSAKELRQIFNQMDTNEDGGLGLFEIKNALKKLDAEMPVHLAYSALCY 61

Query: 96  ADRNRDGKLGEDEIEKFVKYLMSQGYVV 124
           ADRNRDGKLGE+EI++FVKY+MS+GYVV
Sbjct: 62  ADRNRDGKLGEEEIDQFVKYMMSKGYVV 89

[Close]

BLAST of Tan0010525 vs. ExPASy TrEMBL
Match: A0A061FBR4 (Calcium-binding EF-hand family protein, putative OS=Theobroma cacao OX=3641 GN=TCM_033849 PE=4 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 2.5e-09
Identity = 33/71 (46.48%), Postives = 46/71 (64.79%), Query Frame = 0


Query: 52  ELREIFSQMDTNEDGGLGLMEIKDALKRLDAELPVHLAFKALCHADRNRDGKLGEDEIEK 111
           ++R IF++ D N+DG L   E+KDA   L + +P   A+ AL HAD N DG + EDE EK
Sbjct: 46  QVRGIFNRFDANKDGRLSKQELKDAFASLGSRMPGFRAWLALQHADANGDGYISEDEFEK 105

Query: 112 FVKYLMSQGYV 123
            VKY + +GY+
Sbjct: 106 LVKYTLKRGYI 116

[Close]

BLAST of Tan0010525 vs. ExPASy TrEMBL
Match: A0A6A1UYX3 (Putative calcium-binding protein CML10 OS=Morella rubra OX=262757 GN=CJ030_MR0G017070 PE=4 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 4.3e-09
Identity = 38/107 (35.51%), Postives = 59/107 (55.14%), Query Frame = 0


Query: 17  PPLTNTINYRTMSSYGRKSKSKAAAVPSENGYSAKELREIFSQMDTNEDGGLGLMEIKDA 76
           P   +TI  + ++    K   +   VP    Y+ ++LR +F + DTN DG L   E+++A
Sbjct: 2   PAQLHTIRQKNVAYGCSKGMDQYKTVPLP--YTGEQLRGMFKRFDTNGDGQLSRQELRNA 61

Query: 77  LKRLDAELPVHLAFKALCHADRNRDGKLGEDEIEKFVKYLMSQGYVV 124
            + L +  P   AF+AL HADRN DG++ E E++  V Y +  GY V
Sbjct: 62  FRSLGSAAPGWRAFRALGHADRNGDGQISEAELDNLVGYALKHGYTV 106

[Close]

BLAST of Tan0010525 vs. ExPASy TrEMBL
Match: A0A5N6RQD5 (Uncharacterized protein OS=Carpinus fangiana OX=176857 GN=FH972_018542 PE=4 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 5.6e-09
Identity = 35/102 (34.31%), Postives = 57/102 (55.88%), Query Frame = 0


Query: 22  TINYRTMSSYGRKSKSKAAAVPSENGYSAKELREIFSQMDTNEDGGLGLMEIKDALKRLD 81
           TI  + +++   K+K K   +P    Y+  +L  +F + D N DG L   E+++A   L 
Sbjct: 6   TICQKNVANGCHKAKIKTVPMP----YTEAQLMGMFRRFDANGDGHLSRQELRNAFSTLG 65

Query: 82  AELPVHLAFKALCHADRNRDGKLGEDEIEKFVKYLMSQGYVV 124
           +  P   AF+ALCHAD+N DG + E E++  V+Y +  GY +
Sbjct: 66  SSAPGWRAFRALCHADKNGDGYISEAELDNLVRYALKHGYTI 103

The following BLAST results are available for this feature:

BLAST of Tan0010525 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q32NH8	9.0e-04	31.34	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-4 OS=Xenopus lae...	[more]

BLAST of Tan0010525 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6608479.1	3.1e-38	78.10	hypothetical protein SDJN03_01821, partial [Cucurbita argyrosperma subsp. sorori...	[more]
KAG7037807.1	4.1e-38	79.61	hypothetical protein SDJN02_01438, partial [Cucurbita argyrosperma subsp. argyro...	[more]
KGN55187.1	5.7e-32	73.68	hypothetical protein Csa_012728 [Cucumis sativus]	[more]
KAA0064378.1	4.9e-31	77.27	putative calcium-binding protein CML10 [Cucumis melo var. makuwa] >TYK20209.1 pu...	[more]
KAG7024250.1	1.1e-27	76.25	hypothetical protein SDJN02_13064, partial [Cucurbita argyrosperma subsp. argyro...	[more]

BLAST of Tan0010525 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L3J7	2.8e-32	73.68	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G639750 PE=4 SV=1	[more]
A0A5D3D9N2	2.4e-31	77.27	Putative calcium-binding protein CML10 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A061FBR4	2.5e-09	46.48	Calcium-binding EF-hand family protein, putative OS=Theobroma cacao OX=3641 GN=T...	[more]
A0A6A1UYX3	4.3e-09	35.51	Putative calcium-binding protein CML10 OS=Morella rubra OX=262757 GN=CJ030_MR0G0...	[more]
A0A5N6RQD5	5.6e-09	34.31	Uncharacterized protein OS=Carpinus fangiana OX=176857 GN=FH972_018542 PE=4 SV=1	[more]

BLAST of Tan0010525 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 88..116 e-value: 6.0 score: 11.5 coord: 52..80 e-value: 0.038 score: 23.1
IPR002048	EF-hand domain	PFAM	PF13833	EF-hand_8	coord: 48..79 e-value: 0.001 score: 18.8
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 48..83 score: 11.947029
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 84..119 score: 9.073802
None	No IPR available	GENE3D	1.10.238.10		coord: 21..121 e-value: 1.7E-11 score: 46.0
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 97..109
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 51..117

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Tan0010525.1	Tan0010525.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding