Tan0004819 (gene) Snake gourd v1

Overview


Name	Tan0004819
Type	gene
Organism	Trichosanthes anguina (Snake gourd v1)
Description	Calcium-binding EF-hand family protein
Location	LG11: 1475644 .. 1476066 (-)
RNA-Seq Expression	Tan0004819
Synteny	Tan0004819

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSexonpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGACAAAATCGCCTTGTACGAGAAGGTGTTCAAGCAGGTCGACGGCGACGGGGACGGGAAGCTGTCTCCGCCGGAACTGCAGCGGTGCATGGCGGCCGTCGGGGGGAGTTTGACGCTGGAAGAGGCAGAGATGGTGGTGGAGAATTTGGATTCCGACGGCGATGGATTGGTGGGATGGGCTGAATTTGTTGGGTTTGTGGAAGGAGTGGGAGAGGAAGAGAAAGTGAGTGATTTGAAGGAGGCGTTTAGGATGTATGAAATGGATGGTTGTGGGTTCATAACGACGAAGAGTTTGAAGAGGATGTTGAGTAGGCTTGGGGAATCGAGGAGCATTGAGGAATGCAAGAAAATGATTGCTAAATTTGATCTCAATTCTGATGGTGTTCTTAATTTTGATGAGTTTAAGTTCATGATGTCCTGA

mRNA sequence

ATGGACAAAATCGCCTTGTACGAGAAGGTGTTCAAGCAGGTCGACGGCGACGGGGACGGGAAGCTGTCTCCGCCGGAACTGCAGCGGTGCATGGCGGCCGTCGGGGGGAGTTTGACGCTGGAAGAGGCAGAGATGGTGGTGGAGAATTTGGATTCCGACGGCGATGGATTGGTGGGATGGGCTGAATTTGTTGGGTTTGTGGAAGGAGTGGGAGAGGAAGAGAAAGTGAGTGATTTGAAGGAGGCGTTTAGGATGTATGAAATGGATGGTTGTGGGTTCATAACGACGAAGAGTTTGAAGAGGATGTTGAGTAGGCTTGGGGAATCGAGGAGCATTGAGGAATGCAAGAAAATGATTGCTAAATTTGATCTCAATTCTGATGGTGTTCTTAATTTTGATGAGTTTAAGTTCATGATGTCCTGA

Coding sequence (CDS)

ATGGACAAAATCGCCTTGTACGAGAAGGTGTTCAAGCAGGTCGACGGCGACGGGGACGGGAAGCTGTCTCCGCCGGAACTGCAGCGGTGCATGGCGGCCGTCGGGGGGAGTTTGACGCTGGAAGAGGCAGAGATGGTGGTGGAGAATTTGGATTCCGACGGCGATGGATTGGTGGGATGGGCTGAATTTGTTGGGTTTGTGGAAGGAGTGGGAGAGGAAGAGAAAGTGAGTGATTTGAAGGAGGCGTTTAGGATGTATGAAATGGATGGTTGTGGGTTCATAACGACGAAGAGTTTGAAGAGGATGTTGAGTAGGCTTGGGGAATCGAGGAGCATTGAGGAATGCAAGAAAATGATTGCTAAATTTGATCTCAATTCTGATGGTGTTCTTAATTTTGATGAGTTTAAGTTCATGATGTCCTGA

Protein sequence

MDKIALYEKVFKQVDGDGDGKLSPPELQRCMAAVGGSLTLEEAEMVVENLDSDGDGLVGWAEFVGFVEGVGEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIAKFDLNSDGVLNFDEFKFMMS

Homology

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BLAST of Tan0004819 vs. ExPASy Swiss-Prot
Match: Q8RYJ8 (Putative calcium-binding protein CML19 OS=Oryza sativa subsp. japonica OX=39947 GN=CML19 PE=3 SV=1)

HSP 1 Score: 131.3 bits (329), Expect = 8.3e-30
Identity = 72/139 (51.80%), Postives = 94/139 (67.63%), Query Frame = 0


Query: 5   ALYEKVFKQVDGDGDGKLSPPELQRCM-AAVGGSLTLEEAEMVVENLDSDGDGLVGWAEF 64
           A + +VF   D D DGK+S  EL+ CM AA+G  ++ EEAE +V + D+D DGL+   EF
Sbjct: 6   AEFRRVFSAFDRDADGKISAAELRLCMKAALGEDMSAEEAEALVSSADTDDDGLLDEEEF 65

Query: 65  VGF---VEGVGEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 124
                 +E   EEE+   L EAFRMYEM+G G IT  SLKRMLS+LG  + IEEC+ MI 
Sbjct: 66  TKLAVQLEMGDEEERCRGLMEAFRMYEMEGEGRITPASLKRMLSKLGSHQGIEECQTMIC 125

Query: 125 KFDLNSDGVLNFDEFKFMM 140
           +FDL+ DGV++F+EFK MM
Sbjct: 126 RFDLDGDGVISFEEFKIMM 144

[Close]

BLAST of Tan0004819 vs. ExPASy Swiss-Prot
Match: Q9FIH9 (Calcium-binding protein CML37 OS=Arabidopsis thaliana OX=3702 GN=CML37 PE=1 SV=1)

HSP 1 Score: 129.4 bits (324), Expect = 3.1e-29
Identity = 68/132 (51.52%), Postives = 90/132 (68.18%), Query Frame = 0


Query: 10  VFKQVDGDGDGKLSPPELQRCMAAVGGSLTLEEAEMVVENLDSDGDGLVGWAEFVGFVEG 69
           VF  +D + DGK+S  ELQ C++ +GG+L+  E E VV+  D DGDG + + EF+  +EG
Sbjct: 53  VFDYMDANSDGKISGEELQSCVSLLGGALSSREVEEVVKTSDVDGDGFIDFEEFLKLMEG 112

Query: 70  V--GEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIAKFDLNSD 129
               +EE+  +LKEAF MY M+G  FIT  SL+R LSRLGES +++ CK MI  FD N D
Sbjct: 113 EDGSDEERRKELKEAFGMYVMEGEEFITAASLRRTLSRLGESCTVDACKVMIRGFDQNDD 172

Query: 130 GVLNFDEFKFMM 140
           GVL+FDEF  MM
Sbjct: 173 GVLSFDEFVLMM 184

[Close]

BLAST of Tan0004819 vs. ExPASy Swiss-Prot
Match: Q8RYK0 (Probable calcium-binding protein CML31 OS=Oryza sativa subsp. japonica OX=39947 GN=CML31 PE=2 SV=1)

HSP 1 Score: 125.9 bits (315), Expect = 3.5e-28
Identity = 68/136 (50.00%), Postives = 93/136 (68.38%), Query Frame = 0


Query: 10  VFKQVDGDGDGKLSPPELQRCM-AAVGGSLTLEEAEMVVENLDSDGDGLVGWAEFVGFVE 69
           +F   D DGDG++S  EL+ CM   +G  ++ EEA  +V ++D+DGDGL+  AEFV  V+
Sbjct: 15  LFATFDHDGDGRISAAELRLCMKTTLGEEVSDEEAGQLVASVDADGDGLLCEAEFVRLVQ 74

Query: 70  GVGEEE----KVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIAKFDL 129
               EE    + + L+EAF MYEM+G G IT  SL+RML RLG  + I++C+ MI +FDL
Sbjct: 75  AAEVEEEDERRGTGLREAFGMYEMEGEGCITPTSLRRMLRRLGSDQDIDDCRAMICRFDL 134

Query: 130 NSDGVLNFDEFKFMMS 141
           N DGVL+FDEFK MM+
Sbjct: 135 NGDGVLSFDEFKIMMN 150

[Close]

BLAST of Tan0004819 vs. ExPASy Swiss-Prot
Match: Q9SRE6 (Calcium-binding protein CML38 OS=Arabidopsis thaliana OX=3702 GN=CML38 PE=1 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 4.5e-28
Identity = 66/138 (47.83%), Postives = 91/138 (65.94%), Query Frame = 0


Query: 2   DKIALYEKVFKQVDGDGDGKLSPPELQRCMAAVGGSLTLEEAEMVVENLDSDGDGLVGWA 61
           DK    E VF  +D + DG++SP ELQ+    +G  L+ EEA   V   D+DGDG++ + 
Sbjct: 39  DKNRELEAVFSYMDANRDGRISPEELQKSFMTLGEQLSDEEAVAAVRLSDTDGDGMLDFE 98

Query: 62  EFVGFVEGVGEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIAK 121
           EF   ++   EEEK  +LK AFR+Y  +G   IT +SLK ML +LGESR+ ++C+ MI+ 
Sbjct: 99  EFSQLIKVDDEEEKKMELKGAFRLYIAEGEDCITPRSLKMMLKKLGESRTTDDCRVMISA 158

Query: 122 FDLNSDGVLNFDEFKFMM 140
           FDLN+DGVL+FDEF  MM
Sbjct: 159 FDLNADGVLSFDEFALMM 176

[Close]

BLAST of Tan0004819 vs. ExPASy Swiss-Prot
Match: Q8RYJ9 (Putative calcium-binding protein CML23 OS=Oryza sativa subsp. japonica OX=39947 GN=CML23 PE=3 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 2.5e-26
Identity = 69/143 (48.25%), Postives = 91/143 (63.64%), Query Frame = 0


Query: 7   YEKVFKQVDGDGDGKLSPPELQRCM-AAVGGSLTLEEAEMVVENLDSDGDGLVGWAEFVG 66
           + +VF   D DGDGK+S  EL+ C+ A++G  +  EE + ++   D+DGDGL+   EFV 
Sbjct: 7   FRRVFGSFDQDGDGKISATELRLCVKASLGEDMPDEEVQALMALADTDGDGLLDEEEFVR 66

Query: 67  FV---EGVGEEEKVSD------LKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECK 126
            V   E  G+EE+  D      L+EAF MYEM+G G IT  SLK MLS+LG    + EC+
Sbjct: 67  LVTEMEADGDEEEDDDDETCRCLREAFAMYEMEGRGCITPLSLKLMLSKLGTHLDVAECQ 126

Query: 127 KMIAKFDLNSDGVLNFDEFKFMM 140
            MI +FD+N DGVL FDEFK MM
Sbjct: 127 AMICRFDMNGDGVLTFDEFKTMM 149

[Close]

BLAST of Tan0004819 vs. NCBI nr
Match: XP_022133861.1 (putative calcium-binding protein CML19 [Momordica charantia])

HSP 1 Score: 265.8 bits (678), Expect = 2.1e-67
Identity = 132/140 (94.29%), Postives = 135/140 (96.43%), Query Frame = 0


Query: 1   MDKIALYEKVFKQVDGDGDGKLSPPELQRCMAAVGGSLTLEEAEMVVENLDSDGDGLVGW 60
           MDK+ALYEKVF QVDGDGDGKLSPPELQ+CMAAVGGSLTLEEAE+VV NLDSDGDGLVGW
Sbjct: 1   MDKLALYEKVFNQVDGDGDGKLSPPELQQCMAAVGGSLTLEEAELVVGNLDSDGDGLVGW 60

Query: 61  AEFVGFVEGVGEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 120
           AEFV FVEGV EEEKV DLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA
Sbjct: 61  AEFVAFVEGVKEEEKVRDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 120

Query: 121 KFDLNSDGVLNFDEFKFMMS 141
           KFDLNSDGVLNFDEFKFMMS
Sbjct: 121 KFDLNSDGVLNFDEFKFMMS 140

[Close]

BLAST of Tan0004819 vs. NCBI nr
Match: XP_022955599.1 (putative calcium-binding protein CML19 [Cucurbita moschata] >XP_022955608.1 putative calcium-binding protein CML19 [Cucurbita moschata] >KAG6602674.1 putative calcium-binding protein CML19, partial [Cucurbita argyrosperma subsp. sororia] >KAG7033361.1 putative calcium-binding protein CML19, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 265.4 bits (677), Expect = 2.8e-67
Identity = 133/140 (95.00%), Postives = 134/140 (95.71%), Query Frame = 0


Query: 1   MDKIALYEKVFKQVDGDGDGKLSPPELQRCMAAVGGSLTLEEAEMVVENLDSDGDGLVGW 60
           MDKIALYEKVFKQVDGDGDGKLS PELQRCMA VGGSLTLEEAE VVENLDSDGDGLV W
Sbjct: 1   MDKIALYEKVFKQVDGDGDGKLSLPELQRCMAVVGGSLTLEEAEKVVENLDSDGDGLVSW 60

Query: 61  AEFVGFVEGVGEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 120
           AEFV F+EGVGEEEKVSDL EAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA
Sbjct: 61  AEFVAFMEGVGEEEKVSDLMEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 120

Query: 121 KFDLNSDGVLNFDEFKFMMS 141
           KFDLNSDGVLNFDEFKFMMS
Sbjct: 121 KFDLNSDGVLNFDEFKFMMS 140

[Close]

BLAST of Tan0004819 vs. NCBI nr
Match: XP_022938684.1 (putative calcium-binding protein CML19 [Cucurbita moschata] >XP_023550776.1 putative calcium-binding protein CML19 isoform X2 [Cucurbita pepo subsp. pepo] >KAG7015957.1 putative calcium-binding protein CML19, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 264.2 bits (674), Expect = 6.2e-67
Identity = 131/140 (93.57%), Postives = 134/140 (95.71%), Query Frame = 0


Query: 1   MDKIALYEKVFKQVDGDGDGKLSPPELQRCMAAVGGSLTLEEAEMVVENLDSDGDGLVGW 60
           MDK+ALYEKVFKQVDGDGDGKLSP ELQRCMA VGGSLTLEEAEMVVENLD+DGDGL+GW
Sbjct: 2   MDKMALYEKVFKQVDGDGDGKLSPLELQRCMAGVGGSLTLEEAEMVVENLDTDGDGLMGW 61

Query: 61  AEFVGFVEGVGEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 120
            EFV FVEGVGEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEEC KMIA
Sbjct: 62  GEFVAFVEGVGEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECNKMIA 121

Query: 121 KFDLNSDGVLNFDEFKFMMS 141
           KFDLNSDGVLNFDEF FMMS
Sbjct: 122 KFDLNSDGVLNFDEFMFMMS 141

[Close]

BLAST of Tan0004819 vs. NCBI nr
Match: KAG6578374.1 (Protoporphyrinogen oxidase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 264.2 bits (674), Expect = 6.2e-67
Identity = 131/140 (93.57%), Postives = 134/140 (95.71%), Query Frame = 0


Query: 1    MDKIALYEKVFKQVDGDGDGKLSPPELQRCMAAVGGSLTLEEAEMVVENLDSDGDGLVGW 60
            MDK+ALYEKVFKQVDGDGDGKLSP ELQRCMA VGGSLTLEEAEMVVENLD+DGDGL+GW
Sbjct: 1309 MDKMALYEKVFKQVDGDGDGKLSPLELQRCMAGVGGSLTLEEAEMVVENLDTDGDGLMGW 1368

Query: 61   AEFVGFVEGVGEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 120
             EFV FVEGVGEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEEC KMIA
Sbjct: 1369 GEFVAFVEGVGEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECNKMIA 1428

Query: 121  KFDLNSDGVLNFDEFKFMMS 141
            KFDLNSDGVLNFDEF FMMS
Sbjct: 1429 KFDLNSDGVLNFDEFMFMMS 1448

[Close]

BLAST of Tan0004819 vs. NCBI nr
Match: XP_023550775.1 (putative calcium-binding protein CML19 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 264.2 bits (674), Expect = 6.2e-67
Identity = 131/140 (93.57%), Postives = 134/140 (95.71%), Query Frame = 0


Query: 1   MDKIALYEKVFKQVDGDGDGKLSPPELQRCMAAVGGSLTLEEAEMVVENLDSDGDGLVGW 60
           MDK+ALYEKVFKQVDGDGDGKLSP ELQRCMA VGGSLTLEEAEMVVENLD+DGDGL+GW
Sbjct: 58  MDKMALYEKVFKQVDGDGDGKLSPLELQRCMAGVGGSLTLEEAEMVVENLDTDGDGLMGW 117

Query: 61  AEFVGFVEGVGEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 120
            EFV FVEGVGEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEEC KMIA
Sbjct: 118 GEFVAFVEGVGEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECNKMIA 177

Query: 121 KFDLNSDGVLNFDEFKFMMS 141
           KFDLNSDGVLNFDEF FMMS
Sbjct: 178 KFDLNSDGVLNFDEFMFMMS 197

[Close]

BLAST of Tan0004819 vs. ExPASy TrEMBL
Match: A0A6J1BWC8 (putative calcium-binding protein CML19 OS=Momordica charantia OX=3673 GN=LOC111006309 PE=4 SV=1)

HSP 1 Score: 265.8 bits (678), Expect = 1.0e-67
Identity = 132/140 (94.29%), Postives = 135/140 (96.43%), Query Frame = 0


Query: 1   MDKIALYEKVFKQVDGDGDGKLSPPELQRCMAAVGGSLTLEEAEMVVENLDSDGDGLVGW 60
           MDK+ALYEKVF QVDGDGDGKLSPPELQ+CMAAVGGSLTLEEAE+VV NLDSDGDGLVGW
Sbjct: 1   MDKLALYEKVFNQVDGDGDGKLSPPELQQCMAAVGGSLTLEEAELVVGNLDSDGDGLVGW 60

Query: 61  AEFVGFVEGVGEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 120
           AEFV FVEGV EEEKV DLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA
Sbjct: 61  AEFVAFVEGVKEEEKVRDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 120

Query: 121 KFDLNSDGVLNFDEFKFMMS 141
           KFDLNSDGVLNFDEFKFMMS
Sbjct: 121 KFDLNSDGVLNFDEFKFMMS 140

[Close]

BLAST of Tan0004819 vs. ExPASy TrEMBL
Match: A0A6J1GWQ9 (putative calcium-binding protein CML19 OS=Cucurbita moschata OX=3662 GN=LOC111457555 PE=4 SV=1)

HSP 1 Score: 265.4 bits (677), Expect = 1.4e-67
Identity = 133/140 (95.00%), Postives = 134/140 (95.71%), Query Frame = 0


Query: 1   MDKIALYEKVFKQVDGDGDGKLSPPELQRCMAAVGGSLTLEEAEMVVENLDSDGDGLVGW 60
           MDKIALYEKVFKQVDGDGDGKLS PELQRCMA VGGSLTLEEAE VVENLDSDGDGLV W
Sbjct: 1   MDKIALYEKVFKQVDGDGDGKLSLPELQRCMAVVGGSLTLEEAEKVVENLDSDGDGLVSW 60

Query: 61  AEFVGFVEGVGEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 120
           AEFV F+EGVGEEEKVSDL EAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA
Sbjct: 61  AEFVAFMEGVGEEEKVSDLMEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 120

Query: 121 KFDLNSDGVLNFDEFKFMMS 141
           KFDLNSDGVLNFDEFKFMMS
Sbjct: 121 KFDLNSDGVLNFDEFKFMMS 140

[Close]

BLAST of Tan0004819 vs. ExPASy TrEMBL
Match: A0A6J1FET5 (putative calcium-binding protein CML19 OS=Cucurbita moschata OX=3662 GN=LOC111444836 PE=4 SV=1)

HSP 1 Score: 264.2 bits (674), Expect = 3.0e-67
Identity = 131/140 (93.57%), Postives = 134/140 (95.71%), Query Frame = 0


Query: 1   MDKIALYEKVFKQVDGDGDGKLSPPELQRCMAAVGGSLTLEEAEMVVENLDSDGDGLVGW 60
           MDK+ALYEKVFKQVDGDGDGKLSP ELQRCMA VGGSLTLEEAEMVVENLD+DGDGL+GW
Sbjct: 2   MDKMALYEKVFKQVDGDGDGKLSPLELQRCMAGVGGSLTLEEAEMVVENLDTDGDGLMGW 61

Query: 61  AEFVGFVEGVGEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 120
            EFV FVEGVGEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEEC KMIA
Sbjct: 62  GEFVAFVEGVGEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECNKMIA 121

Query: 121 KFDLNSDGVLNFDEFKFMMS 141
           KFDLNSDGVLNFDEF FMMS
Sbjct: 122 KFDLNSDGVLNFDEFMFMMS 141

[Close]

BLAST of Tan0004819 vs. ExPASy TrEMBL
Match: A0A6J1JZS3 (putative calcium-binding protein CML19 OS=Cucurbita maxima OX=3661 GN=LOC111488916 PE=4 SV=1)

HSP 1 Score: 263.8 bits (673), Expect = 4.0e-67
Identity = 131/140 (93.57%), Postives = 134/140 (95.71%), Query Frame = 0


Query: 1   MDKIALYEKVFKQVDGDGDGKLSPPELQRCMAAVGGSLTLEEAEMVVENLDSDGDGLVGW 60
           MDK+ALYEKVFKQVDGDGDGKLSP ELQRCMA VGGSLTLEEAEM VENLD+DGDGL+GW
Sbjct: 2   MDKMALYEKVFKQVDGDGDGKLSPLELQRCMAGVGGSLTLEEAEMAVENLDTDGDGLMGW 61

Query: 61  AEFVGFVEGVGEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 120
            EFV FVE VGEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA
Sbjct: 62  GEFVAFVEEVGEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 121

Query: 121 KFDLNSDGVLNFDEFKFMMS 141
           KFDLNSDGVLNFDEFKFMMS
Sbjct: 122 KFDLNSDGVLNFDEFKFMMS 141

[Close]

BLAST of Tan0004819 vs. ExPASy TrEMBL
Match: A0A6J1JS06 (putative calcium-binding protein CML19 OS=Cucurbita maxima OX=3661 GN=LOC111487817 PE=4 SV=1)

HSP 1 Score: 262.3 bits (669), Expect = 1.1e-66
Identity = 132/140 (94.29%), Postives = 133/140 (95.00%), Query Frame = 0


Query: 1   MDKIALYEKVFKQVDGDGDGKLSPPELQRCMAAVGGSLTLEEAEMVVENLDSDGDGLVGW 60
           MDKIALYEKVFKQVDGDGDGKLS PELQRCMA VGGSLTLEEAE VVENLDSDGDGLV W
Sbjct: 1   MDKIALYEKVFKQVDGDGDGKLSLPELQRCMAVVGGSLTLEEAEKVVENLDSDGDGLVSW 60

Query: 61  AEFVGFVEGVGEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 120
           AEFV F+E VGEEEKVSDL EAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA
Sbjct: 61  AEFVAFMEEVGEEEKVSDLMEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 120

Query: 121 KFDLNSDGVLNFDEFKFMMS 141
           KFDLNSDGVLNFDEFKFMMS
Sbjct: 121 KFDLNSDGVLNFDEFKFMMS 140

[Close]

BLAST of Tan0004819 vs. TAIR 10
Match: AT5G42380.1 (calmodulin like 37 )

HSP 1 Score: 129.4 bits (324), Expect = 2.2e-30
Identity = 68/132 (51.52%), Postives = 90/132 (68.18%), Query Frame = 0


Query: 10  VFKQVDGDGDGKLSPPELQRCMAAVGGSLTLEEAEMVVENLDSDGDGLVGWAEFVGFVEG 69
           VF  +D + DGK+S  ELQ C++ +GG+L+  E E VV+  D DGDG + + EF+  +EG
Sbjct: 53  VFDYMDANSDGKISGEELQSCVSLLGGALSSREVEEVVKTSDVDGDGFIDFEEFLKLMEG 112

Query: 70  V--GEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIAKFDLNSD 129
               +EE+  +LKEAF MY M+G  FIT  SL+R LSRLGES +++ CK MI  FD N D
Sbjct: 113 EDGSDEERRKELKEAFGMYVMEGEEFITAASLRRTLSRLGESCTVDACKVMIRGFDQNDD 172

Query: 130 GVLNFDEFKFMM 140
           GVL+FDEF  MM
Sbjct: 173 GVLSFDEFVLMM 184

[Close]

BLAST of Tan0004819 vs. TAIR 10
Match: AT1G76650.1 (calmodulin-like 38 )

HSP 1 Score: 125.6 bits (314), Expect = 3.2e-29
Identity = 66/138 (47.83%), Postives = 91/138 (65.94%), Query Frame = 0


Query: 2   DKIALYEKVFKQVDGDGDGKLSPPELQRCMAAVGGSLTLEEAEMVVENLDSDGDGLVGWA 61
           DK    E VF  +D + DG++SP ELQ+    +G  L+ EEA   V   D+DGDG++ + 
Sbjct: 39  DKNRELEAVFSYMDANRDGRISPEELQKSFMTLGEQLSDEEAVAAVRLSDTDGDGMLDFE 98

Query: 62  EFVGFVEGVGEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIAK 121
           EF   ++   EEEK  +LK AFR+Y  +G   IT +SLK ML +LGESR+ ++C+ MI+ 
Sbjct: 99  EFSQLIKVDDEEEKKMELKGAFRLYIAEGEDCITPRSLKMMLKKLGESRTTDDCRVMISA 158

Query: 122 FDLNSDGVLNFDEFKFMM 140
           FDLN+DGVL+FDEF  MM
Sbjct: 159 FDLNADGVLSFDEFALMM 176

[Close]

BLAST of Tan0004819 vs. TAIR 10
Match: AT1G76650.3 (calmodulin-like 38 )

HSP 1 Score: 125.6 bits (314), Expect = 3.2e-29
Identity = 66/138 (47.83%), Postives = 91/138 (65.94%), Query Frame = 0


Query: 2   DKIALYEKVFKQVDGDGDGKLSPPELQRCMAAVGGSLTLEEAEMVVENLDSDGDGLVGWA 61
           DK    E VF  +D + DG++SP ELQ+    +G  L+ EEA   V   D+DGDG++ + 
Sbjct: 39  DKNRELEAVFSYMDANRDGRISPEELQKSFMTLGEQLSDEEAVAAVRLSDTDGDGMLDFE 98

Query: 62  EFVGFVEGVGEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIAK 121
           EF   ++   EEEK  +LK AFR+Y  +G   IT +SLK ML +LGESR+ ++C+ MI+ 
Sbjct: 99  EFSQLIKVDDEEEKKMELKGAFRLYIAEGEDCITPRSLKMMLKKLGESRTTDDCRVMISA 158

Query: 122 FDLNSDGVLNFDEFKFMM 140
           FDLN+DGVL+FDEF  MM
Sbjct: 159 FDLNADGVLSFDEFALMM 176

[Close]

BLAST of Tan0004819 vs. TAIR 10
Match: AT1G76640.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 116.3 bits (290), Expect = 2.0e-26
Identity = 65/135 (48.15%), Postives = 86/135 (63.70%), Query Frame = 0


Query: 8   EKVFKQVDGDGDGKLSPPELQRCMAAVGGSLTLEEAEMVVENLDSDGDGLVGWAEFVGFV 67
           E VF  +D + DG++S  EL++    +G  ++ EEAE  V+  D DGDG++   EF   +
Sbjct: 24  EAVFAYMDANRDGRISAEELKKSFKTLGEQMSDEEAEAAVKLSDIDGDGMLDINEFALLI 83

Query: 68  EG---VGEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIAKFDL 127
           +G     EEEK   + EAFRMY  DG   IT  SLK ML +LGESR+ ++CK MI  FDL
Sbjct: 84  KGNDEFTEEEKKRKIMEAFRMYIADGEDCITPGSLKMMLMKLGESRTTDDCKVMIQAFDL 143

Query: 128 NSDGVLNFDEFKFMM 140
           N+DGVL+FDEF  MM
Sbjct: 144 NADGVLSFDEFALMM 158

[Close]

BLAST of Tan0004819 vs. TAIR 10
Match: AT5G37770.1 (EF hand calcium-binding protein family )

HSP 1 Score: 115.5 bits (288), Expect = 3.3e-26
Identity = 61/138 (44.20%), Postives = 90/138 (65.22%), Query Frame = 0


Query: 8   EKVFKQVDGDGDGKLSPPELQRCMAAVGGSLTLEEAEMVVENLDSDGDGLVGWAEFVG-F 67
           +KVF++ D +GDGK+S  EL+  + A+  + + EE   +++  D DG+G +   EFV  F
Sbjct: 19  KKVFQRFDKNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLDGNGFIDLDEFVALF 78

Query: 68  VEGVG----EEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIAKF 127
             G+G        VSDLKEAF +Y++DG G I+ K L  ++  LGE  S+++CKKMI+K 
Sbjct: 79  QIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGEKCSVQDCKKMISKV 138

Query: 128 DLNSDGVLNFDEFKFMMS 141
           D++ DG +NFDEFK MMS
Sbjct: 139 DIDGDGCVNFDEFKKMMS 156

The following BLAST results are available for this feature:

BLAST of Tan0004819 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8RYJ8	8.3e-30	51.80	Putative calcium-binding protein CML19 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q9FIH9	3.1e-29	51.52	Calcium-binding protein CML37 OS=Arabidopsis thaliana OX=3702 GN=CML37 PE=1 SV=1	[more]
Q8RYK0	3.5e-28	50.00	Probable calcium-binding protein CML31 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q9SRE6	4.5e-28	47.83	Calcium-binding protein CML38 OS=Arabidopsis thaliana OX=3702 GN=CML38 PE=1 SV=1	[more]
Q8RYJ9	2.5e-26	48.25	Putative calcium-binding protein CML23 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]

BLAST of Tan0004819 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022133861.1	2.1e-67	94.29	putative calcium-binding protein CML19 [Momordica charantia]	[more]
XP_022955599.1	2.8e-67	95.00	putative calcium-binding protein CML19 [Cucurbita moschata] >XP_022955608.1 puta...	[more]
XP_022938684.1	6.2e-67	93.57	putative calcium-binding protein CML19 [Cucurbita moschata] >XP_023550776.1 puta...	[more]
KAG6578374.1	6.2e-67	93.57	Protoporphyrinogen oxidase 2, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023550775.1	6.2e-67	93.57	putative calcium-binding protein CML19 isoform X1 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Tan0004819 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BWC8	1.0e-67	94.29	putative calcium-binding protein CML19 OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A6J1GWQ9	1.4e-67	95.00	putative calcium-binding protein CML19 OS=Cucurbita moschata OX=3662 GN=LOC11145...	[more]
A0A6J1FET5	3.0e-67	93.57	putative calcium-binding protein CML19 OS=Cucurbita moschata OX=3662 GN=LOC11144...	[more]
A0A6J1JZS3	4.0e-67	93.57	putative calcium-binding protein CML19 OS=Cucurbita maxima OX=3661 GN=LOC1114889...	[more]
A0A6J1JS06	1.1e-66	94.29	putative calcium-binding protein CML19 OS=Cucurbita maxima OX=3661 GN=LOC1114878...	[more]

BLAST of Tan0004819 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G42380.1	2.2e-30	51.52	calmodulin like 37	[more]
AT1G76650.1	3.2e-29	47.83	calmodulin-like 38	[more]
AT1G76650.3	3.2e-29	47.83	calmodulin-like 38	[more]
AT1G76640.1	2.0e-26	48.15	Calcium-binding EF-hand family protein	[more]
AT5G37770.1	3.3e-26	44.20	EF hand calcium-binding protein family	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 78..106 e-value: 0.28 score: 20.2 coord: 114..140 e-value: 0.26 score: 20.3 coord: 6..34 e-value: 0.0034 score: 26.6 coord: 42..70 e-value: 30.0 score: 5.9
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 6..67 e-value: 5.0E-12 score: 46.1 coord: 78..139 e-value: 3.1E-11 score: 43.6
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 38..73 score: 10.831213
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 74..109 score: 11.444912
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 2..37 score: 13.397589
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 110..140 score: 14.318138
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 78..140 e-value: 3.26433E-15 score: 63.7209
None	No IPR available	GENE3D	1.10.238.10		coord: 71..140 e-value: 1.0E-19 score: 72.5
None	No IPR available	GENE3D	1.10.238.10		coord: 1..70 e-value: 1.5E-15 score: 59.1
None	No IPR available	PANTHER	PTHR10891:SF858	EF HAND CALCIUM-BINDING FAMILY PROTEIN	coord: 2..140
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 2..140
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 123..135
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 15..27
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 51..63
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 4..139

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Tan0004819.1	Tan0004819.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016310	phosphorylation
molecular_function	GO:0005509	calcium ion binding
molecular_function	GO:0016301	kinase activity