Tan0004819 (gene) Snake gourd v1

Overview
NameTan0004819
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptionCalcium-binding EF-hand family protein
LocationLG11: 1475644 .. 1476066 (-)
RNA-Seq ExpressionTan0004819
SyntenyTan0004819
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSexonpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACAAAATCGCCTTGTACGAGAAGGTGTTCAAGCAGGTCGACGGCGACGGGGACGGGAAGCTGTCTCCGCCGGAACTGCAGCGGTGCATGGCGGCCGTCGGGGGGAGTTTGACGCTGGAAGAGGCAGAGATGGTGGTGGAGAATTTGGATTCCGACGGCGATGGATTGGTGGGATGGGCTGAATTTGTTGGGTTTGTGGAAGGAGTGGGAGAGGAAGAGAAAGTGAGTGATTTGAAGGAGGCGTTTAGGATGTATGAAATGGATGGTTGTGGGTTCATAACGACGAAGAGTTTGAAGAGGATGTTGAGTAGGCTTGGGGAATCGAGGAGCATTGAGGAATGCAAGAAAATGATTGCTAAATTTGATCTCAATTCTGATGGTGTTCTTAATTTTGATGAGTTTAAGTTCATGATGTCCTGA

mRNA sequence

ATGGACAAAATCGCCTTGTACGAGAAGGTGTTCAAGCAGGTCGACGGCGACGGGGACGGGAAGCTGTCTCCGCCGGAACTGCAGCGGTGCATGGCGGCCGTCGGGGGGAGTTTGACGCTGGAAGAGGCAGAGATGGTGGTGGAGAATTTGGATTCCGACGGCGATGGATTGGTGGGATGGGCTGAATTTGTTGGGTTTGTGGAAGGAGTGGGAGAGGAAGAGAAAGTGAGTGATTTGAAGGAGGCGTTTAGGATGTATGAAATGGATGGTTGTGGGTTCATAACGACGAAGAGTTTGAAGAGGATGTTGAGTAGGCTTGGGGAATCGAGGAGCATTGAGGAATGCAAGAAAATGATTGCTAAATTTGATCTCAATTCTGATGGTGTTCTTAATTTTGATGAGTTTAAGTTCATGATGTCCTGA

Coding sequence (CDS)

ATGGACAAAATCGCCTTGTACGAGAAGGTGTTCAAGCAGGTCGACGGCGACGGGGACGGGAAGCTGTCTCCGCCGGAACTGCAGCGGTGCATGGCGGCCGTCGGGGGGAGTTTGACGCTGGAAGAGGCAGAGATGGTGGTGGAGAATTTGGATTCCGACGGCGATGGATTGGTGGGATGGGCTGAATTTGTTGGGTTTGTGGAAGGAGTGGGAGAGGAAGAGAAAGTGAGTGATTTGAAGGAGGCGTTTAGGATGTATGAAATGGATGGTTGTGGGTTCATAACGACGAAGAGTTTGAAGAGGATGTTGAGTAGGCTTGGGGAATCGAGGAGCATTGAGGAATGCAAGAAAATGATTGCTAAATTTGATCTCAATTCTGATGGTGTTCTTAATTTTGATGAGTTTAAGTTCATGATGTCCTGA

Protein sequence

MDKIALYEKVFKQVDGDGDGKLSPPELQRCMAAVGGSLTLEEAEMVVENLDSDGDGLVGWAEFVGFVEGVGEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIAKFDLNSDGVLNFDEFKFMMS
Homology
[Close]
BLAST of Tan0004819 vs. ExPASy Swiss-Prot
Match: Q8RYJ8 (Putative calcium-binding protein CML19 OS=Oryza sativa subsp. japonica OX=39947 GN=CML19 PE=3 SV=1)

HSP 1 Score: 131.3 bits (329), Expect = 8.3e-30
Identity = 72/139 (51.80%), Postives = 94/139 (67.63%), Query Frame = 0 

Query: 5   ALYEKVFKQVDGDGDGKLSPPELQRCM-AAVGGSLTLEEAEMVVENLDSDGDGLVGWAEF 64
           A + +VF   D D DGK+S  EL+ CM AA+G  ++ EEAE +V + D+D DGL+   EF
Sbjct: 6   AEFRRVFSAFDRDADGKISAAELRLCMKAALGEDMSAEEAEALVSSADTDDDGLLDEEEF 65

Query: 65  VGF---VEGVGEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 124
                 +E   EEE+   L EAFRMYEM+G G IT  SLKRMLS+LG  + IEEC+ MI 
Sbjct: 66  TKLAVQLEMGDEEERCRGLMEAFRMYEMEGEGRITPASLKRMLSKLGSHQGIEECQTMIC 125

Query: 125 KFDLNSDGVLNFDEFKFMM 140
           +FDL+ DGV++F+EFK MM
Sbjct: 126 RFDLDGDGVISFEEFKIMM 144

[Close]
BLAST of Tan0004819 vs. ExPASy Swiss-Prot
Match: Q9FIH9 (Calcium-binding protein CML37 OS=Arabidopsis thaliana OX=3702 GN=CML37 PE=1 SV=1)

HSP 1 Score: 129.4 bits (324), Expect = 3.1e-29
Identity = 68/132 (51.52%), Postives = 90/132 (68.18%), Query Frame = 0 

Query: 10  VFKQVDGDGDGKLSPPELQRCMAAVGGSLTLEEAEMVVENLDSDGDGLVGWAEFVGFVEG 69
           VF  +D + DGK+S  ELQ C++ +GG+L+  E E VV+  D DGDG + + EF+  +EG
Sbjct: 53  VFDYMDANSDGKISGEELQSCVSLLGGALSSREVEEVVKTSDVDGDGFIDFEEFLKLMEG 112

Query: 70  V--GEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIAKFDLNSD 129
               +EE+  +LKEAF MY M+G  FIT  SL+R LSRLGES +++ CK MI  FD N D
Sbjct: 113 EDGSDEERRKELKEAFGMYVMEGEEFITAASLRRTLSRLGESCTVDACKVMIRGFDQNDD 172

Query: 130 GVLNFDEFKFMM 140
           GVL+FDEF  MM
Sbjct: 173 GVLSFDEFVLMM 184

[Close]
BLAST of Tan0004819 vs. ExPASy Swiss-Prot
Match: Q8RYK0 (Probable calcium-binding protein CML31 OS=Oryza sativa subsp. japonica OX=39947 GN=CML31 PE=2 SV=1)

HSP 1 Score: 125.9 bits (315), Expect = 3.5e-28
Identity = 68/136 (50.00%), Postives = 93/136 (68.38%), Query Frame = 0 

Query: 10  VFKQVDGDGDGKLSPPELQRCM-AAVGGSLTLEEAEMVVENLDSDGDGLVGWAEFVGFVE 69
           +F   D DGDG++S  EL+ CM   +G  ++ EEA  +V ++D+DGDGL+  AEFV  V+
Sbjct: 15  LFATFDHDGDGRISAAELRLCMKTTLGEEVSDEEAGQLVASVDADGDGLLCEAEFVRLVQ 74

Query: 70  GVGEEE----KVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIAKFDL 129
               EE    + + L+EAF MYEM+G G IT  SL+RML RLG  + I++C+ MI +FDL
Sbjct: 75  AAEVEEEDERRGTGLREAFGMYEMEGEGCITPTSLRRMLRRLGSDQDIDDCRAMICRFDL 134

Query: 130 NSDGVLNFDEFKFMMS 141
           N DGVL+FDEFK MM+
Sbjct: 135 NGDGVLSFDEFKIMMN 150

[Close]
BLAST of Tan0004819 vs. ExPASy Swiss-Prot
Match: Q9SRE6 (Calcium-binding protein CML38 OS=Arabidopsis thaliana OX=3702 GN=CML38 PE=1 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 4.5e-28
Identity = 66/138 (47.83%), Postives = 91/138 (65.94%), Query Frame = 0 

Query: 2   DKIALYEKVFKQVDGDGDGKLSPPELQRCMAAVGGSLTLEEAEMVVENLDSDGDGLVGWA 61
           DK    E VF  +D + DG++SP ELQ+    +G  L+ EEA   V   D+DGDG++ + 
Sbjct: 39  DKNRELEAVFSYMDANRDGRISPEELQKSFMTLGEQLSDEEAVAAVRLSDTDGDGMLDFE 98

Query: 62  EFVGFVEGVGEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIAK 121
           EF   ++   EEEK  +LK AFR+Y  +G   IT +SLK ML +LGESR+ ++C+ MI+ 
Sbjct: 99  EFSQLIKVDDEEEKKMELKGAFRLYIAEGEDCITPRSLKMMLKKLGESRTTDDCRVMISA 158

Query: 122 FDLNSDGVLNFDEFKFMM 140
           FDLN+DGVL+FDEF  MM
Sbjct: 159 FDLNADGVLSFDEFALMM 176

[Close]
BLAST of Tan0004819 vs. ExPASy Swiss-Prot
Match: Q8RYJ9 (Putative calcium-binding protein CML23 OS=Oryza sativa subsp. japonica OX=39947 GN=CML23 PE=3 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 2.5e-26
Identity = 69/143 (48.25%), Postives = 91/143 (63.64%), Query Frame = 0 

Query: 7   YEKVFKQVDGDGDGKLSPPELQRCM-AAVGGSLTLEEAEMVVENLDSDGDGLVGWAEFVG 66
           + +VF   D DGDGK+S  EL+ C+ A++G  +  EE + ++   D+DGDGL+   EFV 
Sbjct: 7   FRRVFGSFDQDGDGKISATELRLCVKASLGEDMPDEEVQALMALADTDGDGLLDEEEFVR 66

Query: 67  FV---EGVGEEEKVSD------LKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECK 126
            V   E  G+EE+  D      L+EAF MYEM+G G IT  SLK MLS+LG    + EC+
Sbjct: 67  LVTEMEADGDEEEDDDDETCRCLREAFAMYEMEGRGCITPLSLKLMLSKLGTHLDVAECQ 126

Query: 127 KMIAKFDLNSDGVLNFDEFKFMM 140
            MI +FD+N DGVL FDEFK MM
Sbjct: 127 AMICRFDMNGDGVLTFDEFKTMM 149

[Close]
BLAST of Tan0004819 vs. NCBI nr
Match: XP_022133861.1 (putative calcium-binding protein CML19 [Momordica charantia])

HSP 1 Score: 265.8 bits (678), Expect = 2.1e-67
Identity = 132/140 (94.29%), Postives = 135/140 (96.43%), Query Frame = 0 

Query: 1   MDKIALYEKVFKQVDGDGDGKLSPPELQRCMAAVGGSLTLEEAEMVVENLDSDGDGLVGW 60
           MDK+ALYEKVF QVDGDGDGKLSPPELQ+CMAAVGGSLTLEEAE+VV NLDSDGDGLVGW
Sbjct: 1   MDKLALYEKVFNQVDGDGDGKLSPPELQQCMAAVGGSLTLEEAELVVGNLDSDGDGLVGW 60

Query: 61  AEFVGFVEGVGEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 120
           AEFV FVEGV EEEKV DLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA
Sbjct: 61  AEFVAFVEGVKEEEKVRDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 120

Query: 121 KFDLNSDGVLNFDEFKFMMS 141
           KFDLNSDGVLNFDEFKFMMS
Sbjct: 121 KFDLNSDGVLNFDEFKFMMS 140

[Close]
BLAST of Tan0004819 vs. NCBI nr
Match: XP_022955599.1 (putative calcium-binding protein CML19 [Cucurbita moschata] >XP_022955608.1 putative calcium-binding protein CML19 [Cucurbita moschata] >KAG6602674.1 putative calcium-binding protein CML19, partial [Cucurbita argyrosperma subsp. sororia] >KAG7033361.1 putative calcium-binding protein CML19, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 265.4 bits (677), Expect = 2.8e-67
Identity = 133/140 (95.00%), Postives = 134/140 (95.71%), Query Frame = 0 

Query: 1   MDKIALYEKVFKQVDGDGDGKLSPPELQRCMAAVGGSLTLEEAEMVVENLDSDGDGLVGW 60
           MDKIALYEKVFKQVDGDGDGKLS PELQRCMA VGGSLTLEEAE VVENLDSDGDGLV W
Sbjct: 1   MDKIALYEKVFKQVDGDGDGKLSLPELQRCMAVVGGSLTLEEAEKVVENLDSDGDGLVSW 60

Query: 61  AEFVGFVEGVGEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 120
           AEFV F+EGVGEEEKVSDL EAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA
Sbjct: 61  AEFVAFMEGVGEEEKVSDLMEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 120

Query: 121 KFDLNSDGVLNFDEFKFMMS 141
           KFDLNSDGVLNFDEFKFMMS
Sbjct: 121 KFDLNSDGVLNFDEFKFMMS 140

[Close]
BLAST of Tan0004819 vs. NCBI nr
Match: XP_022938684.1 (putative calcium-binding protein CML19 [Cucurbita moschata] >XP_023550776.1 putative calcium-binding protein CML19 isoform X2 [Cucurbita pepo subsp. pepo] >KAG7015957.1 putative calcium-binding protein CML19, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 264.2 bits (674), Expect = 6.2e-67
Identity = 131/140 (93.57%), Postives = 134/140 (95.71%), Query Frame = 0 

Query: 1   MDKIALYEKVFKQVDGDGDGKLSPPELQRCMAAVGGSLTLEEAEMVVENLDSDGDGLVGW 60
           MDK+ALYEKVFKQVDGDGDGKLSP ELQRCMA VGGSLTLEEAEMVVENLD+DGDGL+GW
Sbjct: 2   MDKMALYEKVFKQVDGDGDGKLSPLELQRCMAGVGGSLTLEEAEMVVENLDTDGDGLMGW 61

Query: 61  AEFVGFVEGVGEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 120
            EFV FVEGVGEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEEC KMIA
Sbjct: 62  GEFVAFVEGVGEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECNKMIA 121

Query: 121 KFDLNSDGVLNFDEFKFMMS 141
           KFDLNSDGVLNFDEF FMMS
Sbjct: 122 KFDLNSDGVLNFDEFMFMMS 141

[Close]
BLAST of Tan0004819 vs. NCBI nr
Match: KAG6578374.1 (Protoporphyrinogen oxidase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 264.2 bits (674), Expect = 6.2e-67
Identity = 131/140 (93.57%), Postives = 134/140 (95.71%), Query Frame = 0 

Query: 1    MDKIALYEKVFKQVDGDGDGKLSPPELQRCMAAVGGSLTLEEAEMVVENLDSDGDGLVGW 60
            MDK+ALYEKVFKQVDGDGDGKLSP ELQRCMA VGGSLTLEEAEMVVENLD+DGDGL+GW
Sbjct: 1309 MDKMALYEKVFKQVDGDGDGKLSPLELQRCMAGVGGSLTLEEAEMVVENLDTDGDGLMGW 1368

Query: 61   AEFVGFVEGVGEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 120
             EFV FVEGVGEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEEC KMIA
Sbjct: 1369 GEFVAFVEGVGEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECNKMIA 1428

Query: 121  KFDLNSDGVLNFDEFKFMMS 141
            KFDLNSDGVLNFDEF FMMS
Sbjct: 1429 KFDLNSDGVLNFDEFMFMMS 1448

[Close]
BLAST of Tan0004819 vs. NCBI nr
Match: XP_023550775.1 (putative calcium-binding protein CML19 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 264.2 bits (674), Expect = 6.2e-67
Identity = 131/140 (93.57%), Postives = 134/140 (95.71%), Query Frame = 0 

Query: 1   MDKIALYEKVFKQVDGDGDGKLSPPELQRCMAAVGGSLTLEEAEMVVENLDSDGDGLVGW 60
           MDK+ALYEKVFKQVDGDGDGKLSP ELQRCMA VGGSLTLEEAEMVVENLD+DGDGL+GW
Sbjct: 58  MDKMALYEKVFKQVDGDGDGKLSPLELQRCMAGVGGSLTLEEAEMVVENLDTDGDGLMGW 117

Query: 61  AEFVGFVEGVGEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 120
            EFV FVEGVGEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEEC KMIA
Sbjct: 118 GEFVAFVEGVGEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECNKMIA 177

Query: 121 KFDLNSDGVLNFDEFKFMMS 141
           KFDLNSDGVLNFDEF FMMS
Sbjct: 178 KFDLNSDGVLNFDEFMFMMS 197

[Close]
BLAST of Tan0004819 vs. ExPASy TrEMBL
Match: A0A6J1BWC8 (putative calcium-binding protein CML19 OS=Momordica charantia OX=3673 GN=LOC111006309 PE=4 SV=1)

HSP 1 Score: 265.8 bits (678), Expect = 1.0e-67
Identity = 132/140 (94.29%), Postives = 135/140 (96.43%), Query Frame = 0 

Query: 1   MDKIALYEKVFKQVDGDGDGKLSPPELQRCMAAVGGSLTLEEAEMVVENLDSDGDGLVGW 60
           MDK+ALYEKVF QVDGDGDGKLSPPELQ+CMAAVGGSLTLEEAE+VV NLDSDGDGLVGW
Sbjct: 1   MDKLALYEKVFNQVDGDGDGKLSPPELQQCMAAVGGSLTLEEAELVVGNLDSDGDGLVGW 60

Query: 61  AEFVGFVEGVGEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 120
           AEFV FVEGV EEEKV DLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA
Sbjct: 61  AEFVAFVEGVKEEEKVRDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 120

Query: 121 KFDLNSDGVLNFDEFKFMMS 141
           KFDLNSDGVLNFDEFKFMMS
Sbjct: 121 KFDLNSDGVLNFDEFKFMMS 140

[Close]
BLAST of Tan0004819 vs. ExPASy TrEMBL
Match: A0A6J1GWQ9 (putative calcium-binding protein CML19 OS=Cucurbita moschata OX=3662 GN=LOC111457555 PE=4 SV=1)

HSP 1 Score: 265.4 bits (677), Expect = 1.4e-67
Identity = 133/140 (95.00%), Postives = 134/140 (95.71%), Query Frame = 0 

Query: 1   MDKIALYEKVFKQVDGDGDGKLSPPELQRCMAAVGGSLTLEEAEMVVENLDSDGDGLVGW 60
           MDKIALYEKVFKQVDGDGDGKLS PELQRCMA VGGSLTLEEAE VVENLDSDGDGLV W
Sbjct: 1   MDKIALYEKVFKQVDGDGDGKLSLPELQRCMAVVGGSLTLEEAEKVVENLDSDGDGLVSW 60

Query: 61  AEFVGFVEGVGEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 120
           AEFV F+EGVGEEEKVSDL EAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA
Sbjct: 61  AEFVAFMEGVGEEEKVSDLMEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 120

Query: 121 KFDLNSDGVLNFDEFKFMMS 141
           KFDLNSDGVLNFDEFKFMMS
Sbjct: 121 KFDLNSDGVLNFDEFKFMMS 140

[Close]
BLAST of Tan0004819 vs. ExPASy TrEMBL
Match: A0A6J1FET5 (putative calcium-binding protein CML19 OS=Cucurbita moschata OX=3662 GN=LOC111444836 PE=4 SV=1)

HSP 1 Score: 264.2 bits (674), Expect = 3.0e-67
Identity = 131/140 (93.57%), Postives = 134/140 (95.71%), Query Frame = 0 

Query: 1   MDKIALYEKVFKQVDGDGDGKLSPPELQRCMAAVGGSLTLEEAEMVVENLDSDGDGLVGW 60
           MDK+ALYEKVFKQVDGDGDGKLSP ELQRCMA VGGSLTLEEAEMVVENLD+DGDGL+GW
Sbjct: 2   MDKMALYEKVFKQVDGDGDGKLSPLELQRCMAGVGGSLTLEEAEMVVENLDTDGDGLMGW 61

Query: 61  AEFVGFVEGVGEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 120
            EFV FVEGVGEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEEC KMIA
Sbjct: 62  GEFVAFVEGVGEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECNKMIA 121

Query: 121 KFDLNSDGVLNFDEFKFMMS 141
           KFDLNSDGVLNFDEF FMMS
Sbjct: 122 KFDLNSDGVLNFDEFMFMMS 141

[Close]
BLAST of Tan0004819 vs. ExPASy TrEMBL
Match: A0A6J1JZS3 (putative calcium-binding protein CML19 OS=Cucurbita maxima OX=3661 GN=LOC111488916 PE=4 SV=1)

HSP 1 Score: 263.8 bits (673), Expect = 4.0e-67
Identity = 131/140 (93.57%), Postives = 134/140 (95.71%), Query Frame = 0 

Query: 1   MDKIALYEKVFKQVDGDGDGKLSPPELQRCMAAVGGSLTLEEAEMVVENLDSDGDGLVGW 60
           MDK+ALYEKVFKQVDGDGDGKLSP ELQRCMA VGGSLTLEEAEM VENLD+DGDGL+GW
Sbjct: 2   MDKMALYEKVFKQVDGDGDGKLSPLELQRCMAGVGGSLTLEEAEMAVENLDTDGDGLMGW 61

Query: 61  AEFVGFVEGVGEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 120
            EFV FVE VGEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA
Sbjct: 62  GEFVAFVEEVGEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 121

Query: 121 KFDLNSDGVLNFDEFKFMMS 141
           KFDLNSDGVLNFDEFKFMMS
Sbjct: 122 KFDLNSDGVLNFDEFKFMMS 141

[Close]
BLAST of Tan0004819 vs. ExPASy TrEMBL
Match: A0A6J1JS06 (putative calcium-binding protein CML19 OS=Cucurbita maxima OX=3661 GN=LOC111487817 PE=4 SV=1)

HSP 1 Score: 262.3 bits (669), Expect = 1.1e-66
Identity = 132/140 (94.29%), Postives = 133/140 (95.00%), Query Frame = 0 

Query: 1   MDKIALYEKVFKQVDGDGDGKLSPPELQRCMAAVGGSLTLEEAEMVVENLDSDGDGLVGW 60
           MDKIALYEKVFKQVDGDGDGKLS PELQRCMA VGGSLTLEEAE VVENLDSDGDGLV W
Sbjct: 1   MDKIALYEKVFKQVDGDGDGKLSLPELQRCMAVVGGSLTLEEAEKVVENLDSDGDGLVSW 60

Query: 61  AEFVGFVEGVGEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 120
           AEFV F+E VGEEEKVSDL EAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA
Sbjct: 61  AEFVAFMEEVGEEEKVSDLMEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 120

Query: 121 KFDLNSDGVLNFDEFKFMMS 141
           KFDLNSDGVLNFDEFKFMMS
Sbjct: 121 KFDLNSDGVLNFDEFKFMMS 140

[Close]
BLAST of Tan0004819 vs. TAIR 10
Match: AT5G42380.1 (calmodulin like 37 )

HSP 1 Score: 129.4 bits (324), Expect = 2.2e-30
Identity = 68/132 (51.52%), Postives = 90/132 (68.18%), Query Frame = 0 

Query: 10  VFKQVDGDGDGKLSPPELQRCMAAVGGSLTLEEAEMVVENLDSDGDGLVGWAEFVGFVEG 69
           VF  +D + DGK+S  ELQ C++ +GG+L+  E E VV+  D DGDG + + EF+  +EG
Sbjct: 53  VFDYMDANSDGKISGEELQSCVSLLGGALSSREVEEVVKTSDVDGDGFIDFEEFLKLMEG 112

Query: 70  V--GEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIAKFDLNSD 129
               +EE+  +LKEAF MY M+G  FIT  SL+R LSRLGES +++ CK MI  FD N D
Sbjct: 113 EDGSDEERRKELKEAFGMYVMEGEEFITAASLRRTLSRLGESCTVDACKVMIRGFDQNDD 172

Query: 130 GVLNFDEFKFMM 140
           GVL+FDEF  MM
Sbjct: 173 GVLSFDEFVLMM 184

[Close]
BLAST of Tan0004819 vs. TAIR 10
Match: AT1G76650.1 (calmodulin-like 38 )

HSP 1 Score: 125.6 bits (314), Expect = 3.2e-29
Identity = 66/138 (47.83%), Postives = 91/138 (65.94%), Query Frame = 0 

Query: 2   DKIALYEKVFKQVDGDGDGKLSPPELQRCMAAVGGSLTLEEAEMVVENLDSDGDGLVGWA 61
           DK    E VF  +D + DG++SP ELQ+    +G  L+ EEA   V   D+DGDG++ + 
Sbjct: 39  DKNRELEAVFSYMDANRDGRISPEELQKSFMTLGEQLSDEEAVAAVRLSDTDGDGMLDFE 98

Query: 62  EFVGFVEGVGEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIAK 121
           EF   ++   EEEK  +LK AFR+Y  +G   IT +SLK ML +LGESR+ ++C+ MI+ 
Sbjct: 99  EFSQLIKVDDEEEKKMELKGAFRLYIAEGEDCITPRSLKMMLKKLGESRTTDDCRVMISA 158

Query: 122 FDLNSDGVLNFDEFKFMM 140
           FDLN+DGVL+FDEF  MM
Sbjct: 159 FDLNADGVLSFDEFALMM 176

[Close]
BLAST of Tan0004819 vs. TAIR 10
Match: AT1G76650.3 (calmodulin-like 38 )

HSP 1 Score: 125.6 bits (314), Expect = 3.2e-29
Identity = 66/138 (47.83%), Postives = 91/138 (65.94%), Query Frame = 0 

Query: 2   DKIALYEKVFKQVDGDGDGKLSPPELQRCMAAVGGSLTLEEAEMVVENLDSDGDGLVGWA 61
           DK    E VF  +D + DG++SP ELQ+    +G  L+ EEA   V   D+DGDG++ + 
Sbjct: 39  DKNRELEAVFSYMDANRDGRISPEELQKSFMTLGEQLSDEEAVAAVRLSDTDGDGMLDFE 98

Query: 62  EFVGFVEGVGEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIAK 121
           EF   ++   EEEK  +LK AFR+Y  +G   IT +SLK ML +LGESR+ ++C+ MI+ 
Sbjct: 99  EFSQLIKVDDEEEKKMELKGAFRLYIAEGEDCITPRSLKMMLKKLGESRTTDDCRVMISA 158

Query: 122 FDLNSDGVLNFDEFKFMM 140
           FDLN+DGVL+FDEF  MM
Sbjct: 159 FDLNADGVLSFDEFALMM 176

[Close]
BLAST of Tan0004819 vs. TAIR 10
Match: AT1G76640.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 116.3 bits (290), Expect = 2.0e-26
Identity = 65/135 (48.15%), Postives = 86/135 (63.70%), Query Frame = 0 

Query: 8   EKVFKQVDGDGDGKLSPPELQRCMAAVGGSLTLEEAEMVVENLDSDGDGLVGWAEFVGFV 67
           E VF  +D + DG++S  EL++    +G  ++ EEAE  V+  D DGDG++   EF   +
Sbjct: 24  EAVFAYMDANRDGRISAEELKKSFKTLGEQMSDEEAEAAVKLSDIDGDGMLDINEFALLI 83

Query: 68  EG---VGEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIAKFDL 127
           +G     EEEK   + EAFRMY  DG   IT  SLK ML +LGESR+ ++CK MI  FDL
Sbjct: 84  KGNDEFTEEEKKRKIMEAFRMYIADGEDCITPGSLKMMLMKLGESRTTDDCKVMIQAFDL 143

Query: 128 NSDGVLNFDEFKFMM 140
           N+DGVL+FDEF  MM
Sbjct: 144 NADGVLSFDEFALMM 158

[Close]
BLAST of Tan0004819 vs. TAIR 10
Match: AT5G37770.1 (EF hand calcium-binding protein family )

HSP 1 Score: 115.5 bits (288), Expect = 3.3e-26
Identity = 61/138 (44.20%), Postives = 90/138 (65.22%), Query Frame = 0 

Query: 8   EKVFKQVDGDGDGKLSPPELQRCMAAVGGSLTLEEAEMVVENLDSDGDGLVGWAEFVG-F 67
           +KVF++ D +GDGK+S  EL+  + A+  + + EE   +++  D DG+G +   EFV  F
Sbjct: 19  KKVFQRFDKNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLDGNGFIDLDEFVALF 78

Query: 68  VEGVG----EEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIAKF 127
             G+G        VSDLKEAF +Y++DG G I+ K L  ++  LGE  S+++CKKMI+K 
Sbjct: 79  QIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGEKCSVQDCKKMISKV 138

Query: 128 DLNSDGVLNFDEFKFMMS 141
           D++ DG +NFDEFK MMS
Sbjct: 139 DIDGDGCVNFDEFKKMMS 156

The following BLAST results are available for this feature:
BLAST of Tan0004819 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q8RYJ88.3e-3051.80Putative calcium-binding protein CML19 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
Q9FIH93.1e-2951.52Calcium-binding protein CML37 OS=Arabidopsis thaliana OX=3702 GN=CML37 PE=1 SV=1[more]
Q8RYK03.5e-2850.00Probable calcium-binding protein CML31 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
Q9SRE64.5e-2847.83Calcium-binding protein CML38 OS=Arabidopsis thaliana OX=3702 GN=CML38 PE=1 SV=1[more]
Q8RYJ92.5e-2648.25Putative calcium-binding protein CML23 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
BLAST of Tan0004819 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022133861.12.1e-6794.29putative calcium-binding protein CML19 [Momordica charantia][more]
XP_022955599.12.8e-6795.00putative calcium-binding protein CML19 [Cucurbita moschata] >XP_022955608.1 puta... [more]
XP_022938684.16.2e-6793.57putative calcium-binding protein CML19 [Cucurbita moschata] >XP_023550776.1 puta... [more]
KAG6578374.16.2e-6793.57Protoporphyrinogen oxidase 2, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023550775.16.2e-6793.57putative calcium-binding protein CML19 isoform X1 [Cucurbita pepo subsp. pepo][more]
BLAST of Tan0004819 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1BWC81.0e-6794.29putative calcium-binding protein CML19 OS=Momordica charantia OX=3673 GN=LOC1110... [more]
A0A6J1GWQ91.4e-6795.00putative calcium-binding protein CML19 OS=Cucurbita moschata OX=3662 GN=LOC11145... [more]
A0A6J1FET53.0e-6793.57putative calcium-binding protein CML19 OS=Cucurbita moschata OX=3662 GN=LOC11144... [more]
A0A6J1JZS34.0e-6793.57putative calcium-binding protein CML19 OS=Cucurbita maxima OX=3661 GN=LOC1114889... [more]
A0A6J1JS061.1e-6694.29putative calcium-binding protein CML19 OS=Cucurbita maxima OX=3661 GN=LOC1114878... [more]
BLAST of Tan0004819 vs. TAIR 10
Match NameE-valueIdentityDescription
AT5G42380.12.2e-3051.52calmodulin like 37 [more]
AT1G76650.13.2e-2947.83calmodulin-like 38 [more]
AT1G76650.33.2e-2947.83calmodulin-like 38 [more]
AT1G76640.12.0e-2648.15Calcium-binding EF-hand family protein [more]
AT5G37770.13.3e-2644.20EF hand calcium-binding protein family [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainSMARTSM00054efh_1coord: 78..106
e-value: 0.28
score: 20.2
coord: 114..140
e-value: 0.26
score: 20.3
coord: 6..34
e-value: 0.0034
score: 26.6
coord: 42..70
e-value: 30.0
score: 5.9
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 6..67
e-value: 5.0E-12
score: 46.1
coord: 78..139
e-value: 3.1E-11
score: 43.6
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 38..73
score: 10.831213
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 74..109
score: 11.444912
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 2..37
score: 13.397589
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 110..140
score: 14.318138
IPR002048EF-hand domainCDDcd00051EFhcoord: 78..140
e-value: 3.26433E-15
score: 63.7209
NoneNo IPR availableGENE3D1.10.238.10coord: 71..140
e-value: 1.0E-19
score: 72.5
NoneNo IPR availableGENE3D1.10.238.10coord: 1..70
e-value: 1.5E-15
score: 59.1
NoneNo IPR availablePANTHERPTHR10891:SF858EF HAND CALCIUM-BINDING FAMILY PROTEINcoord: 2..140
NoneNo IPR availablePANTHERPTHR10891EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEINcoord: 2..140
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 123..135
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 15..27
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 51..63
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 4..139

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0004819.1Tan0004819.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016310 phosphorylation
molecular_function GO:0005509 calcium ion binding
molecular_function GO:0016301 kinase activity