Tan0003508 (gene) Snake gourd v1

BLAST of Tan0003508 vs. ExPASy Swiss-Prot
Match: P52706 ((R)-mandelonitrile lyase 1 OS=Prunus serotina OX=23207 GN=MDL1 PE=1 SV=1)

HSP 1 Score: 537.7 bits (1384), Expect = 2.9e-151
Identity = 276/537 (51.40%), Postives = 377/537 (70.20%), Query Frame = 0

Query: 1   MEHSMATILLLIL---ISLFQFG-VLSSHTISDQDVSYMKLVHNASDIPEKEEYEYIIIG 60
           ME S  + +LL+L   + L Q+  V S  T S+ D SY++  ++A+D+  +  Y+Y+I+G
Sbjct: 1   MEKSTMSAILLVLHLFVLLLQYSEVHSLATTSNHDFSYLRFAYDATDLELEGSYDYVIVG 60

Query: 61  GGTAGCPLAATLSSKFSVLLLERGSDPNKYPSVLNEQGLLNAFVAEDDGENPFQRFISED 120
           GGT+GCPLAATLS K+ VL+LERGS P  YP+VL   G +     EDDG+ P +RF+SED
Sbjct: 61  GGTSGCPLAATLSEKYKVLVLERGSLPTAYPNVLTADGFVYNLQQEDDGKTPVERFVSED 120

Query: 121 GVENIRGRVLGGGSMVNAGFYSRGHREFFETAGVDWDMELVNKSYQWVEQTVVSRPILNA 180
           G++N+RGRVLGG SM+NAG Y+R +   +  +GVDWDM+LVNK+Y+WVE T+V +P    
Sbjct: 121 GIDNVRGRVLGGTSMINAGVYARANTSIYSASGVDWDMDLVNKTYEWVEDTIVFKPNYQP 180

Query: 181 WQSAFRTALLEAGVVPDNEFDLKHLVGTKTGGSIFDDKGNRHGAVELLNKAKPENLKVAI 240
           WQS   TA LEAGV P++ F L H  GT+  GS FD+KG RH A ELLNK    NL+V +
Sbjct: 181 WQSVTGTAFLEAGVDPNHGFSLDHEAGTRITGSTFDNKGTRHAADELLNKGNSNNLRVGV 240

Query: 241 QATVQKIIFS---GLSASGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGIGP 300
            A+V+KIIFS   GL+A+GV Y DS G  H AF+R KGE+I+SAG IG+PQLLLLSG+GP
Sbjct: 241 HASVEKIIFSNAPGLTATGVIYRDSNGTPHRAFVRSKGEVIVSAGTIGTPQLLLLSGVGP 300

Query: 301 KSHLSSLKLPVVLHQPHVGESMSDNPRFGANIMLPFQLVASSVKVVGTLKNNIHLQAFAS 360
           +S+LSSL +PVVL  P+VG+ + DNPR   NI+ P  +  + V V+G + N+ +  +F+S
Sbjct: 301 ESYLSSLNIPVVLSHPYVGQFLHDNPRNFINILPPNPIEPTIVTVLG-ISNDFYQCSFSS 360

Query: 361 PSPFLVPPSFSLLPPQSTSIIPS-LALFVGKFSQVLSEGSLRL-NSSNVKKSPIVRFNYY 420
             PF  PP FS  P  S  +  S  A F  K +  LS GSL L +SSNV+ SP V+FNYY
Sbjct: 361 -LPFTTPP-FSFFPSTSYPLPNSTFAHFASKVAGPLSYGSLTLKSSSNVRVSPNVKFNYY 420

Query: 421 SHPDDLAKCVRGVRKVGDLLQTQTMEKIKTQDLEGNKVFQFLGLPWPENLSDDSSVEEYC 480
           S+P DL+ CV G++K+G+LL T  ++  K +DL G + F  LG+P P++ +DD++ E +C
Sbjct: 421 SNPTDLSHCVSGMKKIGELLSTDALKPYKVEDLPGIEGFNILGIPLPKDQTDDAAFETFC 480

Query: 481 QKTVTTYWHYHGGCLVGKVVDGNFKVIGIENLRVVDGSTFSDSPGTNPMATLMMLGR 529
           +++V +YWHYHGGCLVGKV+DG+F+V GI+ LRVVDGSTF  +P ++P    +MLGR
Sbjct: 481 RESVASYWHYHGGCLVGKVLDGDFRVTGIDALRVVDGSTFPYTPASHPQGFYLMLGR 534

BLAST of Tan0003508 vs. ExPASy Swiss-Prot
Match: Q945K2 ((R)-mandelonitrile lyase 2 OS=Prunus dulcis OX=3755 GN=MDL2 PE=1 SV=1)

HSP 1 Score: 528.9 bits (1361), Expect = 1.3e-148
Identity = 272/537 (50.65%), Postives = 373/537 (69.46%), Query Frame = 0

Query: 1   MEHSMATILLLIL----ISLFQFGVLSSHTISDQDVSYMKLVHNASDIPEKEEYEYIIIG 60
           ME S  + +LL+L    + L    V S  T SD D SY+   ++A+D+  +  Y+Y+I+G
Sbjct: 1   MEKSTMSAILLVLYIFVLHLQYSEVHSLATTSDHDFSYLSFAYDATDLELEGSYDYVIVG 60

Query: 61  GGTAGCPLAATLSSKFSVLLLERGSDPNKYPSVLNEQGLLNAFVAEDDGENPFQRFISED 120
           GGT+GCPLAATLS K+ VL+LERGS P  YP+VL   G +     EDDG+ P +RF+SED
Sbjct: 61  GGTSGCPLAATLSEKYKVLVLERGSLPTAYPNVLTADGFVYNLQQEDDGKTPVERFVSED 120

Query: 121 GVENIRGRVLGGGSMVNAGFYSRGHREFFETAGVDWDMELVNKSYQWVEQTVVSRPILNA 180
           G++N+RGRVLGG S++NAG Y+R +   +  +GVDWDM+LVN++Y+WVE T+V +P   +
Sbjct: 121 GIDNVRGRVLGGTSIINAGVYARANTSIYSASGVDWDMDLVNQTYEWVEDTIVYKPNSQS 180

Query: 181 WQSAFRTALLEAGVVPDNEFDLKHLVGTKTGGSIFDDKGNRHGAVELLNKAKPENLKVAI 240
           WQS  +TA LEAGV P++ F L H  GT+  GS FD+KG RH A ELLNK    NL+V +
Sbjct: 181 WQSVTKTAFLEAGVHPNHGFSLDHEEGTRITGSTFDNKGTRHAADELLNKGNSNNLRVGV 240

Query: 241 QATVQKIIFS---GLSASGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGIGP 300
            A+V+KIIFS   GL+A+GV Y DS G  H AF+R KGE+I+SAG IG+PQLLLLSG+GP
Sbjct: 241 HASVEKIIFSNAPGLTATGVIYRDSNGTPHQAFVRSKGEVIVSAGTIGTPQLLLLSGVGP 300

Query: 301 KSHLSSLKLPVVLHQPHVGESMSDNPRFGANIMLPFQLVASSVKVVGTLKNNIHLQAFAS 360
           +S+LSSL +PVVL  P+VG+ + DNPR   NI+ P  +  + V V+G + N+ +  +F+S
Sbjct: 301 ESYLSSLNIPVVLSHPYVGQFLHDNPRNFINILPPNPIEPTIVTVLG-ISNDFYQCSFSS 360

Query: 361 PSPFLVPPSFSLLPPQSTSIIPS-LALFVGKFSQVLSEGSLRL-NSSNVKKSPIVRFNYY 420
             PF  PP F   P  S  +  S  A F  K +  LS GSL L +SSNV+ SP V+FNYY
Sbjct: 361 -LPFTTPP-FGFFPSASYPLPNSTFAHFASKVAGPLSYGSLTLKSSSNVRVSPNVKFNYY 420

Query: 421 SHPDDLAKCVRGVRKVGDLLQTQTMEKIKTQDLEGNKVFQFLGLPWPENLSDDSSVEEYC 480
           S+  DL+ CV G++K+G+LL T  ++  K +DL G + F  LG+P P++ +DD++ E +C
Sbjct: 421 SNLTDLSHCVSGMKKIGELLSTDALKPYKVEDLPGVEGFNILGIPLPKDQTDDAAFETFC 480

Query: 481 QKTVTTYWHYHGGCLVGKVVDGNFKVIGIENLRVVDGSTFSDSPGTNPMATLMMLGR 529
           +++V +YWHYHGGCLVGKV+DG+F+V GI  LRVVDGSTF  +P ++P    +MLGR
Sbjct: 481 RESVASYWHYHGGCLVGKVLDGDFRVTGINALRVVDGSTFPYTPASHPQGFYLMLGR 534

BLAST of Tan0003508 vs. ExPASy Swiss-Prot
Match: P52707 ((R)-mandelonitrile lyase 3 OS=Prunus serotina OX=23207 GN=MDL3 PE=2 SV=1)

HSP 1 Score: 518.1 bits (1333), Expect = 2.3e-145
Identity = 266/537 (49.53%), Postives = 365/537 (67.97%), Query Frame = 0

Query: 1   MEHSMATILLLI---LISLFQFGVLSSHTISDQDVSYMKLVHNASDIPEKEEYEYIIIGG 60
           ++ +M+ +LL++   ++ L    V S    S  D SY+  V++A+D   +  Y+YII+GG
Sbjct: 2   VKSTMSAVLLVLHIFVLHLQYSEVQSLANTSSHDFSYLSFVYDATDPELEGSYDYIIVGG 61

Query: 61  GTAGCPLAATLSSKFSVLLLERGSDPNKYPSVLNEQGLLNAFVAEDDGENPFQRFISEDG 120
           GTAGCPLAATLS+ +SVL+LERGS P +YP++L   G +     EDDG+ P +RF+SEDG
Sbjct: 62  GTAGCPLAATLSANYSVLVLERGSLPTEYPNLLISDGFVYNLQQEDDGKTPVERFVSEDG 121

Query: 121 VENIRGRVLGGGSMVNAGFYSRGHREFFETAGVDWDMELVNKSYQWVEQTVVSRPILNAW 180
           ++N+RGRVLGG SM+NAG Y R +  FF   G++WDM+LVN++Y+WVE T+V  P    W
Sbjct: 122 IDNVRGRVLGGTSMINAGVYVRANTSFFNQTGIEWDMDLVNQTYEWVEDTIVFEPDSQTW 181

Query: 181 QSAFRTALLEAGVVPDNEFDLKHLVGTKTGGSIFDDKGNRHGAVELLNKAKPENLKVAIQ 240
           Q+   TA LEAG++P+N F + HL GT+  GS FD+ G RH + ELLNK  P NL+VA+Q
Sbjct: 182 QTVIGTAYLEAGILPNNGFSVDHLAGTRLTGSTFDNNGTRHASDELLNKGDPNNLRVAVQ 241

Query: 241 ATVQKIIF----SGLSASGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGIGP 300
           A V+KIIF    SG++A GV Y+DS G  H AF+R +GE+ILSAG IGSPQLLLLSG+GP
Sbjct: 242 AAVEKIIFSSNTSGVTAIGVIYTDSNGTTHQAFVRGEGEVILSAGPIGSPQLLLLSGVGP 301

Query: 301 KSHLSSLKLPVVLHQPHVGESMSDNPRFGANIMLPFQLVASSVKVVGTLKNNIHLQAFAS 360
           +S+L+SL + VV   P+VG+ + DNPR   NI+ P  + AS+V V+G   +    Q   S
Sbjct: 302 ESYLTSLNISVVASHPYVGQYIYDNPRNFINILPPNPIEASTVTVLGITSD--FYQCSIS 361

Query: 361 PSPFLVPPSFSLLPPQSTSI-IPSLALFVGKFSQVLSEGSLRLN-SSNVKKSPIVRFNYY 420
             PF  PP FS  P  S  +   + A  V K    LS G++ LN SS+V+  P V+FNYY
Sbjct: 362 SLPFDTPP-FSFFPTTSYPLPNQTFAHIVNKVPGPLSHGTVTLNSSSDVRVGPNVKFNYY 421

Query: 421 SHPDDLAKCVRGVRKVGDLLQTQTMEKIKTQDLEGNKVFQFLGLPWPENLSDDSSVEEYC 480
           S+  DL+ CV G++K+G++L T  +E  K +DL G   F  LG+P PEN +DD++ E +C
Sbjct: 422 SNLTDLSHCVSGMKKLGEVLSTDALEPYKVEDLPGIDGFNILGIPLPENQTDDAAFETFC 481

Query: 481 QKTVTTYWHYHGGCLVGKVVDGNFKVIGIENLRVVDGSTFSDSPGTNPMATLMMLGR 529
           +++V +YWHYHGGCLVGKV+D  F+V GI  LRVVDGSTF  +P ++P    +MLGR
Sbjct: 482 RESVASYWHYHGGCLVGKVLDDGFRVTGINALRVVDGSTFPSTPASHPQGFYLMLGR 535

BLAST of Tan0003508 vs. ExPASy Swiss-Prot
Match: Q9SSM2 ((R)-mandelonitrile lyase-like OS=Arabidopsis thaliana OX=3702 GN=At1g73050 PE=2 SV=1)

HSP 1 Score: 507.7 bits (1306), Expect = 3.2e-142
Identity = 257/535 (48.04%), Postives = 363/535 (67.85%), Query Frame = 0

Query: 6   ATILLLILISLFQFGVL---SSHTISDQDVSYMKLVHNASDIPEKEEYEYIIIGGGTAGC 65
           +++L   L+ L   GV+   ++    ++   +M+ + NA+D   ++ Y+YII+GGGTAGC
Sbjct: 7   SSLLYTALVVLLLLGVVHRSNARPRVNRPPGFMRFISNATDFASEDYYDYIIVGGGTAGC 66

Query: 66  PLAATLSSKFSVLLLERGSDPNKYPSVLNEQGLLNAFVAEDDGENPFQRFISEDGVENIR 125
           PLAATLS  F VLLLERG  P   P+V++  G L      ++ ++P Q FISE+GV N R
Sbjct: 67  PLAATLSQSFRVLLLERGGVPYNRPNVMSHDGFLTTLTDVNNFDSPAQSFISEEGVPNAR 126

Query: 126 GRVLGGGSMVNAGFYSRGHREFFETAGVDWDMELVNKSYQWVEQTVVSRPILNAWQSAFR 185
           GRVLGG S +NAGFYSR  ++FFE +G+ WD+  VN+SY+WVE+ +V RP L  WQ+A R
Sbjct: 127 GRVLGGSSAINAGFYSRADKQFFENSGLVWDLSSVNQSYEWVERAIVFRPQLRTWQTAIR 186

Query: 186 TALLEAGVVPDNEFDLKHLVGTKTGGSIFDDKGNRHGAVELLNKAKPENLKVAIQATVQK 245
            ALLE GV P N F L+H VGTK GGS FD  G RH + +LL  A+  N++VA+ ATV++
Sbjct: 187 DALLEVGVHPFNGFTLEHKVGTKIGGSTFDRTGRRHSSADLLRYARSSNIRVAVYATVER 246

Query: 246 IIF--------SGLSASGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGIGPK 305
           ++         S +SA GV Y D  G+ H A IR +GE+ILSAGA+GSPQLL LSGIGP+
Sbjct: 247 VLLASSPSVSGSNVSAIGVVYRDQLGRFHHALIRDRGEVILSAGALGSPQLLFLSGIGPR 306

Query: 306 SHLSSLKLPVVLHQPHVGESMSDNPRFGANIMLPFQLVASSVKVVGTLKNNIHLQAFASP 365
           S+LS+  +PV L QPHVG+ + DNPR G +I+ P  +  S ++VVG  ++   L+A ++ 
Sbjct: 307 SYLSTWGIPVALDQPHVGDFVYDNPRNGISIVPPVPMENSLIQVVGVTEDGAFLEAASNV 366

Query: 366 SPFLVP-PSFSLLPPQSTSIIPSLALFVGKFSQVLSEGSLRLNSSNVKKSPIVRFNYYSH 425
            PF  P  S  +  P S   +P +   + K    +S G LRL S++V+ +P+VRFNY+S 
Sbjct: 367 IPFASPLHSVFIRAPASPLYVP-VTTIMEKILGPVSIGLLRLASTDVRINPVVRFNYFSD 426

Query: 426 PDDLAKCVRGVRKVGDLLQTQTMEKIKTQDLEGNKVFQFLGLPWPENLSDDSSVEEYCQK 485
           P DL +CV G RK+G++L+++ M+    ++  GN+ F+F+G P P + S+D  + ++C++
Sbjct: 427 PQDLERCVNGTRKIGEILRSRAMQDFMIREWFGNRRFRFVGAPLPVDQSNDLVMADFCRR 486

Query: 486 TVTTYWHYHGGCLVGKVVDGNFKVIGIENLRVVDGSTFSDSPGTNPMATLMMLGR 529
           TV+T WHYHGG +VGKVVD + KVIG+ +LR+VDGSTF+ SPGTNP ATLMMLGR
Sbjct: 487 TVSTIWHYHGGAVVGKVVDSDLKVIGVNSLRLVDGSTFNISPGTNPQATLMMLGR 540

BLAST of Tan0003508 vs. ExPASy Swiss-Prot
Match: O82784 ((R)-mandelonitrile lyase 4 OS=Prunus serotina OX=23207 GN=MDL4 PE=2 SV=1)

HSP 1 Score: 505.0 bits (1299), Expect = 2.1e-141
Identity = 261/533 (48.97%), Postives = 356/533 (66.79%), Query Frame = 0

Query: 4   SMATILLLILISLFQFGVLSS--HTISDQDVSYMKLVHNASDIPEKEEYEYIIIGGGTAG 63
           S   ++L +L+   Q+  + S  +T S+ D  Y+K V+NA D+  +  Y+YII+GGGT+G
Sbjct: 7   SAVVLVLNLLVLHLQYSEVHSLANTSSEHDFGYLKFVYNAVDLELEGSYDYIIVGGGTSG 66

Query: 64  CPLAATLSSKFSVLLLERGSDPNKYPSVLNEQGLLNAFVAEDDGENPFQRFISEDGVENI 123
           CPLAATLS+ +SVL+LERG+   +YP+ L   G       +DDG+ P +RF+SEDG++N+
Sbjct: 67  CPLAATLSANYSVLVLERGTIATEYPNTLTVDGFAYNLQQQDDGKTPVERFVSEDGIDNV 126

Query: 124 RGRVLGGGSMVNAGFYSRGHREFFETAGVDWDMELVNKSYQWVEQTVVSRPILNAWQSAF 183
           R R+LGG +++NAG Y+R +  F+  +GV+WD++LVN++Y+WVE  +V +P   +WQS  
Sbjct: 127 RSRILGGTTIINAGVYARANESFYNNSGVEWDLDLVNEAYEWVEDAIVYKPSNQSWQSIT 186

Query: 184 RTALLEAGVVPDNEFDLKHLVGTKTGGSIFDDKGNRHGAVELLNKAKPENLKVAIQATVQ 243
            TA LEAGV PDN F L H  GT+  GS FD+ G RH + ELLNK  P+NLKVA++A VQ
Sbjct: 187 GTAFLEAGVHPDNGFGLVHEEGTRLTGSTFDNSGTRHASDELLNKGDPDNLKVAVEAAVQ 246

Query: 244 KIIF----SGLSASGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGIGPKSHL 303
           KIIF    SGL+A GV Y+DS G  H A +  KGE+ILSAG +G+PQLLLLSG+GP+S+L
Sbjct: 247 KIIFSTESSGLTAVGVVYTDSNGTSHRALVSGKGEVILSAGTLGTPQLLLLSGVGPESYL 306

Query: 304 SSLKLPVVLHQPHVGESMSDNPRFGANIMLPFQLVASSVKVVGTLKNNIHLQAFASPSPF 363
           +SL + VV   P+VG+ ++DNPR   NI+ P  +  S+V V+G   +    Q   S  PF
Sbjct: 307 TSLNISVVASHPYVGQYVNDNPRNFINILPPNPIEPSTVTVLGITSD--FYQCSLSSLPF 366

Query: 364 LVPPSFSLLPPQSTSI-IPSLALFVGKFSQVLSEGSLRL-NSSNVKKSPIVRFNYYSHPD 423
             PP FSL P  S  +   + A  V K    LS GSL L +SSNV  +P V+FNY S P 
Sbjct: 367 DTPP-FSLFPTTSYPLPNQTFAHIVSKVPGPLSAGSLTLQSSSNVSVAPNVKFNYCSDPV 426

Query: 424 DLAKCVRGVRKVGDLLQTQTMEKIKTQDLEGNKVFQFLGLPWPENLSDDSSVEEYCQKTV 483
           DL  CV G++K+G  L T  ++  K  DL G   F  LG P PEN +DD++ E++C+ TV
Sbjct: 427 DLTHCVSGMKKIGVFLSTDALKPYKVDDLPGIDGFNILGTPLPENQTDDAAFEKFCRDTV 486

Query: 484 TTYWHYHGGCLVGKVVDGNFKVIGIENLRVVDGSTFSDSPGTNPMATLMMLGR 529
            +YWHYHGG +VGKV+DGNF+V GI  LRVVDGSTF  +P ++P    +MLGR
Sbjct: 487 ASYWHYHGGAIVGKVIDGNFRVTGINALRVVDGSTFPATPASHPQGFYLMLGR 536

BLAST of Tan0003508 vs. NCBI nr
Match: KAG7029801.1 ((R)-mandelonitrile lyase 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1503.0 bits (3890), Expect = 0.0e+00
Identity = 748/1029 (72.69%), Postives = 857/1029 (83.28%), Query Frame = 0

Query: 35   MKLVHNASDIPEKEEYEYIIIGGGTAGCPLAATLSSKFSVLLLERGSDPNKYPSVLNEQG 94
            MK V N + +P +EEY+YIIIGGGTAGCPLAATLSS FSVL+LERGSDPN +PSVL++QG
Sbjct: 1    MKFVQNGNTLPTREEYDYIIIGGGTAGCPLAATLSSNFSVLVLERGSDPNAFPSVLSQQG 60

Query: 95   LLNAFVAEDDGENPFQRFISEDGVENIRGRVLGGGSMVNAGFYSRGHREFFETAGVDWDM 154
            L N     DDG NPFQRF+SEDGVENIRGRVLGGGSM+N GFYSR   EFF+++G+ WDM
Sbjct: 61   LANTLTDNDDGLNPFQRFVSEDGVENIRGRVLGGGSMINVGFYSRAQPEFFKSSGIQWDM 120

Query: 155  ELVNKSYQWVEQTVVSRPILNAWQSAFRTALLEAGVVPDNEFDLKHLVGTKTGGSIFDDK 214
              V K+YQW+E+TVVSRP L+ WQSAFR ALLEAGV PDN +DLKH VGT+TGGSIFD +
Sbjct: 121  TAVEKAYQWIEETVVSRPELSPWQSAFREALLEAGVGPDNGYDLKHGVGTRTGGSIFDSR 180

Query: 215  GNRHGAVELLNKAKPENLKVAIQATVQKIIFS---GLSASGVSYSDSKGKLHTAFIRKKG 274
            G RHGAVELLNKA P NL+VA QATV++IIFS   GLSASGV YSD KGKLH A I K G
Sbjct: 181  GRRHGAVELLNKADPRNLRVATQATVKRIIFSQSNGLSASGVLYSDFKGKLHKATISKNG 240

Query: 275  EIILSAGAIGSPQLLLLSGIGPKSHLSSLKLPVVLHQPHVGESMSDNPRFGANIMLPFQL 334
            EIIL+AGAIGSP LLL SG+GPKSHLSSLKLPVVLH  HVG+SM+DNPRFGA I+LPF  
Sbjct: 241  EIILTAGAIGSPHLLLQSGVGPKSHLSSLKLPVVLHNRHVGQSMADNPRFGAAIVLPFLT 300

Query: 335  VASSVKVVGTLKNNIHLQAFASPSPFLVPPSFSLLPPQSTSIIPSLALFVGKFSQVLSEG 394
              +SV+VVGTLK NIH+++ +S  PF + P F LLPP+ST++  SLA+F GKFS V S G
Sbjct: 301  PPTSVQVVGTLKRNIHIESLSSILPFSIFPPFGLLPPRSTAVNLSLAVFAGKFSTVSSVG 360

Query: 395  SLRLNSSNVKKSPIVRFNYYSHPDDLAKCVRGVRKVGDLLQTQTMEKIKTQDLEGNKVFQ 454
            SLRL+    +K+PIVRFNY SHPDD+ +CV GVRKVGDL+ T++ME+IKT DLEG K F+
Sbjct: 361  SLRLDG---RKNPIVRFNYLSHPDDVERCVEGVRKVGDLVNTKSMERIKTGDLEGKKGFK 420

Query: 455  FLGLPWPENLSDDSSVEEYCQKTVTTYWHYHGGCLVGKVVDGNFKVIGIENLRVVDGSTF 514
            FLG P PEN+SD   V ++C+KTVTT+WHYHGGCLVGKVVD N++VIGI+ LRVVDGSTF
Sbjct: 421  FLGAPLPENMSDYKLVGDFCRKTVTTFWHYHGGCLVGKVVDDNYRVIGIKKLRVVDGSTF 480

Query: 515  SDSPGTNPMATLMMLGRLPLQNTKFSMWDKWRMRVGFDVGKLYTKFVHDASDLPEKEEYE 574
            S SPGTNPMAT+MMLGR         M     ++   DV   Y KFVH+A DLPEK+EY+
Sbjct: 481  SLSPGTNPMATVMMLGRY----VGLKM-----LQQRLDVS--YMKFVHNAGDLPEKQEYD 540

Query: 575  YIIIGGGTTGCPLAATLSSKFSVLLLERGSDPNKYPSVLNEQGLLNAFVTEDDGENPFQR 634
            YIIIGGG  GCPLAATLSSKF VLLLERGS+PNKYPSVLNEQGLLNAF+ +DDG+NPFQR
Sbjct: 541  YIIIGGGAAGCPLAATLSSKFKVLLLERGSEPNKYPSVLNEQGLLNAFLAQDDGQNPFQR 600

Query: 635  FISEDGVENIRGRVLGGGSMVNAGFYSRGHREFFETAGVDWDMELVDKSYQWVEQTVVSR 694
            F SEDGVENIRGR+LGGG+MVNAGFYSRGHR+FFETAGV+WDME+V+ +YQWVE+TVVS+
Sbjct: 601  FTSEDGVENIRGRILGGGTMVNAGFYSRGHRQFFETAGVNWDMEMVENAYQWVEETVVSK 660

Query: 695  PILNAWQSVFRSALLEAGVVPDNGFDLRHLVGTKIGGSIFDDKGNRHGVVELLNKAELEN 754
            PILNAWQS F+SALLEAGV PDNGF+L HL+GTK GGSIFD KGNRHG VELLNKAE +N
Sbjct: 661  PILNAWQSAFKSALLEAGVGPDNGFNLTHLIGTKTGGSIFDGKGNRHGAVELLNKAEPKN 720

Query: 755  L----------------------------KLHTAFIRKKGEIILSAGAIGSPQLLLLSGI 814
            L                            K+HTAFIRKKGEI LSAGAIGSP LLL SG+
Sbjct: 721  LKVAVNATVRKILFNGLSANGVSYSDSKGKIHTAFIRKKGEIFLSAGAIGSPLLLLQSGV 780

Query: 815  GPKSHLSSLKLPVVLHQPHVGESMSDNPRFGANIMLPFQLVASSVKVVGTLKNNIHLQAF 874
            GPKSHLSSLKLPVV HQP+VGE MSDNPRFGA I+LPFQL +SSVKVVGTL++N+HLQAF
Sbjct: 781  GPKSHLSSLKLPVVHHQPYVGEFMSDNPRFGATIVLPFQLASSSVKVVGTLQDNVHLQAF 840

Query: 875  ASPSPFLVPPSFSLLPPQSTSIIPSLALFVGKFSQVLSEGSLRLNS-SNVKKSPIVRFNY 934
            ASP+PFL PP+FSLLPPQ+TSI PSL +FVGKFS+V SEG LRLNS ++ K + IVRFNY
Sbjct: 841  ASPAPFLAPPTFSLLPPQATSINPSLVIFVGKFSEVHSEGFLRLNSTTDAKNNVIVRFNY 900

Query: 935  YSHPDDLPKCVRGVRKVGDLLQTQTMVKIKTQDLEGNKVFQFLGPPLPENLSDDITVENY 994
            YSHPDDL +CV GVRKVGDLL+TQTM KIKTQDLEGNK FQF+G PLPENL DD +VE Y
Sbjct: 901  YSHPDDLARCVEGVRKVGDLLKTQTMEKIKTQDLEGNKGFQFMGVPLPENLGDDSSVEEY 960

Query: 995  CKKTVTTYWHYHGGCLIGKVVDGNYRVIGIENLRVVDGSTFSESPGTNPMATLMMLGRYV 1032
            C+KTVTTYWHYHGGCL+GKVVDG+YRVIG++NLRVVDGSTFS+SPGTNPMATLMMLGRYV
Sbjct: 961  CRKTVTTYWHYHGGCLVGKVVDGDYRVIGMKNLRVVDGSTFSDSPGTNPMATLMMLGRYV 1015

BLAST of Tan0003508 vs. NCBI nr
Match: KAF4368106.1 (hypothetical protein G4B88_001010 [Cannabis sativa])

HSP 1 Score: 1044.3 bits (2699), Expect = 7.2e-301
Identity = 557/1064 (52.35%), Postives = 710/1064 (66.73%), Query Frame = 0

Query: 28   SDQDVSYMKLVHNASDIP-EKEEYEYIIIGGGTAGCPLAATLSSKFSVLLLERGSDPNKY 87
            SD D  YMK VH+A+++  E+E Y+YIIIGGGTAGCPLAATLS  +SVL+LERGS P   
Sbjct: 36   SDHDFRYMKSVHDATEMSLEEEYYDYIIIGGGTAGCPLAATLSENYSVLVLERGSVPTAN 95

Query: 88   PSVLNEQGLLNAFVAEDDGE--NPFQRFISEDGVENIRGRVLGGGSMVNAGFYSRGHREF 147
            P+VL+  G     + E++     P QRF SEDGVEN RGRVLGG SM+NAGF+SRG   F
Sbjct: 96   PNVLHLHGFFANLMQEEEETRVTPAQRFTSEDGVENARGRVLGGTSMINAGFFSRGDEGF 155

Query: 148  FETAGVDWDMELVNKSYQWVEQTVVSRPILNAWQSAFRTALLEAGVVPDNEFDLKHLVGT 207
            F   GV W+M+ V K+Y+WVE+++V RP L  WQS+FR ALLE GV PDNEFDL H +GT
Sbjct: 156  FSKPGVKWEMDRVEKAYEWVEESIVFRPKLPLWQSSFRDALLEIGVGPDNEFDLNHKLGT 215

Query: 208  KTGGSIFDDKGNRHGAVELLNKAKPENLKVAIQATVQKIIFSGL---------------- 267
            K  GS FD+ G RHGAVELLNK    NLKV + ATV+KI FS                  
Sbjct: 216  KISGSTFDEVGRRHGAVELLNKGNLNNLKVVVHATVEKIFFSTKVSRNYHSGDDNDHDNN 275

Query: 268  ----SASGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGIGPKSHLSSLKLPV 327
                 A GV YSDSKGK HT  ++ KGE+ILSAGAIGSPQLLLLSGIGP S+LSSL +P+
Sbjct: 276  PPKPYAIGVIYSDSKGKSHTVLVKHKGEVILSAGAIGSPQLLLLSGIGPHSYLSSLNIPI 335

Query: 328  VLHQPHVGESMSDNPRFGANIMLPFQLVASSVKVVGTLKNNIHLQAFASPSP-FLVPPSF 387
            V  QP+VG+ ++DNPR   N+++P     S V+VVG + N+  ++  ++  P  L P  F
Sbjct: 336  VHSQPNVGDFIADNPRNNINLIIPSPTDPSPVQVVG-ITNHYFIETISANLPASLTPLPF 395

Query: 388  SLLPPQSTSIIPSLALFVGKFSQVLSEGSLRLNSSN-VKKSPIVRFNYYSHPDDLAKCVR 447
            S+ P  ST+ +  + +   K  Q LS GSL L S N V+ +P VRFNY+SHP DL++CV 
Sbjct: 396  SVYPKSSTAHL-GMTVICEKLRQPLSSGSLWLASPNDVRVTPHVRFNYFSHPKDLSQCVN 455

Query: 448  GVRKVGDLLQTQTMEKIKTQDLEGNKVFQFLGLPWPENLSDDSSVEEYCQKTVTTYWHYH 507
            GVRK+G++L+T  ME+ K  D    + F + G   P N SD+S++EE+C+ +VTT WHYH
Sbjct: 456  GVRKIGEMLETNAMERFKMVDSNEERNFMYFGPSLPTNQSDESAMEEFCRSSVTTIWHYH 515

Query: 508  GGCLVGKVVDGNFKVIGIENLRVVDGSTFSDSPGTNPMATLMMLGRLPLQNTKFSMWDKW 567
            GGC VGKVVDG+F+V+G+ +LRVVDGSTF  SPGTNP AT+MMLGR         M  + 
Sbjct: 516  GGCTVGKVVDGDFRVMGVNSLRVVDGSTFRVSPGTNPQATVMMLGRY----VGLKMLQER 575

Query: 568  RMRVGFDVGKLYTKFVHDASDLP-EKEEYEYIIIGGGTTGCPLAATLSSKFSVLLLERGS 627
                  +    Y K V+DA+++  E+E Y+YIIIG    GCPLAATLS  +SVL+LERGS
Sbjct: 576  EAEANVE----YMKSVYDATEMSLEEEYYDYIIIG---AGCPLAATLSENYSVLVLERGS 635

Query: 628  DPNKYPSVLNEQGLLNAFVTEDDGE--NPFQRFISEDGVENIRGRVLGGGSMVNAGFYSR 687
             P   P+VL+  G L   + E++     P QRF SEDGVEN+RGRVLGG SM+NAGF+SR
Sbjct: 636  VPTSNPNVLHLSGFLANLMQEEEETRVTPAQRFTSEDGVENVRGRVLGGSSMINAGFFSR 695

Query: 688  GHREFFETAGVDWDMELVDKSYQWVEQTVVSRPILNAWQSVFRSALLEAGVVPDNGFDLR 747
            G   F+  +GV W+M+ V+K+Y+WVE+++V RP L  WQS FR ALLE GV P+NGFDL 
Sbjct: 696  GDEGFYSKSGVKWEMDRVEKAYEWVEESIVFRPKLPVWQSSFRDALLEVGVGPNNGFDLN 755

Query: 748  HLVGTKIGGSIFDDKGNRHGVVELLNKAELENLKL------------------------- 807
            H +GTKI GS FD+ G RHG VELLNK  L NLK+                         
Sbjct: 756  HKLGTKISGSTFDEVGRRHGAVELLNKGNLNNLKVGVHATVDRIIFSTKVSNNYHSGDDS 815

Query: 808  --------HTAFIRKKGEIILSAGAIGSPQLLLLSGIGPKSHLSSLKLPVVLHQPHVGES 867
                    HT  +R KGEIILSAGAIGSPQLLLLSGIGP S+LSSL +P+VL QP+VG+ 
Sbjct: 816  DQNSPRKSHTVLVRHKGEIILSAGAIGSPQLLLLSGIGPHSYLSSLNIPIVLSQPNVGDF 875

Query: 868  MSDNPRFGANIMLPFQLVASSVKVVGTLKNNIHLQAFASPSPFLVPPSFSLLPPQSTSII 927
            ++DNPR   N+++PF    S+V+VVG   +       A+      P  FS+ P  ST+ +
Sbjct: 876  IADNPRNNINLIIPFPTEPSAVQVVGITDHYFMETISANLPNSPTPLPFSMYPKSSTANL 935

Query: 928  PSLALFVGKFSQVLSEGSLRLNSSN-VKKSPIVRFNYYSHPDDLPKCVRGVRKVGDLLQT 987
              +A+   K    LS GSL L SSN V+ +P VRFNY+SHP DL +CV  VRK+G++L +
Sbjct: 936  -GMAIICEKLRHPLSSGSLWLASSNDVRVTPHVRFNYFSHPKDLSQCVSAVRKIGEVLSS 995

Query: 988  QTMVKIKTQDLEGNKVFQFLGPPLPENLSDDITVENYCKKTVTTYWHYHGGCLIGKVVDG 1030
            ++M + K  D    + F + GP LP N SD+  +E +C+ +VTT WHYHGGC +GKVVDG
Sbjct: 996  KSMERFKMVDSNEERNFMYFGPSLPTNQSDESAMEEFCRSSVTTIWHYHGGCTVGKVVDG 1055

BLAST of Tan0003508 vs. NCBI nr
Match: KAF4349168.1 (hypothetical protein F8388_026317 [Cannabis sativa])

HSP 1 Score: 1039.3 bits (2686), Expect = 2.3e-299
Identity = 565/1125 (50.22%), Postives = 724/1125 (64.36%), Query Frame = 0

Query: 28   SDQDVSYMKLVHNASDIP-EKEEYEYIIIGGGTAGCPLAATLSSKFSVLLLERGSDPNKY 87
            SD D  YMK VH+A+++  E+E Y+YIIIGGGTAGCPLAATLS  +SVL+LERGS P   
Sbjct: 36   SDHDFRYMKSVHDATEMSLEEEYYDYIIIGGGTAGCPLAATLSENYSVLVLERGSVPTAN 95

Query: 88   PSVLNEQGLLNAFVAEDDGE--NPFQRFISEDGVENIRGRVLGGGSMVNAGFYSRGHREF 147
            P+VL+  G     + E++     P QRF SEDGVEN RGRVLGG SM+NAGF+SRG   F
Sbjct: 96   PNVLHLHGFFANLMQEEEETRVTPAQRFTSEDGVENARGRVLGGTSMINAGFFSRGDEGF 155

Query: 148  FETAGVDWDMELVNKSYQWVEQTVVSRPILNAWQSAFRTALLEAGVVPDNEFDLKHLVGT 207
            F   GV W+M+ V K+Y+WVE+++V RP L  WQS+FR ALLE GV PDNEFDL H +GT
Sbjct: 156  FSKPGVKWEMDRVEKAYEWVEESIVFRPKLPLWQSSFRDALLEIGVGPDNEFDLNHKLGT 215

Query: 208  KTGGSIFDDKGNRHGAVELLNKAKPENLKVAIQATVQKIIFSGL---------------- 267
            K  GS FD+ G RHGAVELLNK    NLKV + ATV+KI FS                  
Sbjct: 216  KISGSTFDEVGRRHGAVELLNKGNLNNLKVVVHATVEKIFFSTKVSRNYHSGDDNDHDNN 275

Query: 268  ----SASGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGIGPKSHLSSLKLPV 327
                 A GV YSDSKGK HT  ++ KGE+ILSAGAIGSPQLLLLSGIGP S+LSSL +P+
Sbjct: 276  PPKPYAIGVIYSDSKGKSHTVLVKHKGEVILSAGAIGSPQLLLLSGIGPHSYLSSLNIPI 335

Query: 328  VLHQPHVGESMSDNPRFGANIMLPFQLVASSVKVVGTLKNNIHLQAFASPSP-FLVPPSF 387
            V  QP+VG+ ++DNPR   N+++P     S V+VVG + N+  ++  ++  P  L P  F
Sbjct: 336  VHSQPNVGDFIADNPRNNINLIIPSPTDPSPVQVVG-ITNHYFIETISANLPASLTPLPF 395

Query: 388  SLLPPQSTSIIPSLALFVGKFSQVLSEGSLRLNSSN-VKKSPIVRFNYYSHPDDLAKCVR 447
            S+ P  ST+ +  + +   K  Q LS GSL L S N V+ +P VRFNY+SHP DL++CV 
Sbjct: 396  SVYPKSSTAHL-GMTVICEKLRQPLSSGSLWLASPNDVRVTPHVRFNYFSHPKDLSQCVN 455

Query: 448  GVRKVGDLLQTQTMEKIKTQDLEGNKVFQFLGLPWPENLSDDSSVEEYCQKTVTTYWHYH 507
            GVRK+G++L+T  ME+ K  D    + F + G   P N SD+S++EE+C+ +VTT WHYH
Sbjct: 456  GVRKIGEMLETNAMERFKMVDSNEERNFMYFGPSLPTNQSDESAMEEFCRSSVTTIWHYH 515

Query: 508  GGCLVGKVVDGNFKVIGIENLRVVDGSTFSDSPGTNPMATLMMLGR-------------- 567
            GGC VGKVVDG+F+V+G+ +LRVVDGSTF  SPGTNP AT+MMLGR              
Sbjct: 516  GGCTVGKVVDGDFRVMGVNSLRVVDGSTFRVSPGTNPQATVMMLGRYVGLKMLQEREAEA 575

Query: 568  -----------------------LPLQNTKFSMWDKWRMRV-----GFDVGKLYTKFVHD 627
                                   + L+ ++++  D  +  +     G D    Y K V+D
Sbjct: 576  NVEGDIERREIDEKDRVKDYLSTVALRVSRWATSDGQQQVLALATSGSDHDFRYMKSVYD 635

Query: 628  ASDLP-EKEEYEYIIIGGG--TTGCPLAATLSSKFSVLLLERGSDPNKYPSVLNEQGLLN 687
            A+++  E+E Y+YIIIGGG  T GCPLAATLS  +SVL+LERGS P   P+VL+  G L 
Sbjct: 636  ATEMSLEEEYYDYIIIGGGTLTAGCPLAATLSENYSVLVLERGSVPTSNPNVLHLSGFLA 695

Query: 688  AFVTEDDGE--NPFQRFISEDGVENIRGRVLGGGSMVNAGFYSRGHREFFETAGVDWDME 747
              + E++     P QRF SEDGVEN+RGRVLGG SM+NAGF+SRG   F+  +GV W+M+
Sbjct: 696  NLMQEEEETRVTPAQRFTSEDGVENVRGRVLGGSSMINAGFFSRGDEGFYSKSGVKWEMD 755

Query: 748  LVDKSYQWVEQTVVSRPILNAWQSVFRSALLEAGVVPDNGFDLRHLVGTKIGGSIFDDKG 807
             V+K+Y+WVE+++V RP L  WQS FR ALLE GV P+NGFDL H +GTKI GS FD+ G
Sbjct: 756  RVEKAYEWVEESIVFRPKLPVWQSSFRDALLEVGVGPNNGFDLNHKLGTKISGSTFDEVG 815

Query: 808  NRHGVVELLNKAELENLKL----------------------------------------- 867
             RHG VELLNK  L NLK+                                         
Sbjct: 816  RRHGAVELLNKGNLNNLKVGVHATVDRIIFSTKVSNNYHSGDDSDQNSPKPYAIGVMYSD 875

Query: 868  -----HTAFIRKKGEIILSAGAIGSPQLLLLSGIGPKSHLSSLKLPVVLHQPHVGESMSD 927
                 HT  +R KGEIILSAGAIGSPQLLLLSGIGP S+LSSL +P+VL QP+VG+ ++D
Sbjct: 876  SKGKSHTVLVRHKGEIILSAGAIGSPQLLLLSGIGPHSYLSSLNIPIVLSQPNVGDFIAD 935

Query: 928  NPRFGANIMLPFQLVASSVKVVGTLKNNIHLQAFASPSPFLVPPSFSLLPPQSTSIIPSL 987
            NPR   N+++PF    S+V+VVG   +       A+      P  FS+ P  ST+ +  +
Sbjct: 936  NPRNNINLIIPFPTEPSAVQVVGITDHYFMETISANLPNSPTPLPFSMYPKSSTANL-GM 995

Query: 988  ALFVGKFSQVLSEGSLRLNSSN-VKKSPIVRFNYYSHPDDLPKCVRGVRKVGDLLQTQTM 1034
            A+   K    LS GSL L SSN V+ +P VRFNY+SHP DL +CV  VRK+G++L +++M
Sbjct: 996  AIICEKLRHPLSSGSLWLASSNDVRVTPHVRFNYFSHPKDLSQCVSAVRKIGEVLSSKSM 1055

BLAST of Tan0003508 vs. NCBI nr
Match: KAF4383855.1 (hypothetical protein G4B88_016288 [Cannabis sativa])

HSP 1 Score: 1036.9 bits (2680), Expect = 1.1e-298
Identity = 559/1073 (52.10%), Postives = 733/1073 (68.31%), Query Frame = 0

Query: 1    MEHSMATIL-LLILISLFQFGVLSSHTISDQDVSYMKLVHNASDIPEKEEYEYIIIGGGT 60
            ME S  +IL LLI ++ F    L   + +D D SYMK V+NA+D+P +EEY+YI++GGGT
Sbjct: 1    MESSSLSILTLLIFLTCFDHAKLLV-SGADYDFSYMKSVYNATDMPLEEEYDYIVVGGGT 60

Query: 61   AGCPLAATLSSKFSVLLLERGSDPNKYPSVLNEQGLLNAFVA--EDDGENPFQRFISEDG 120
            AGCPLAATLS K+SVL+LERG+ P  +P+ L   G L   V   +DDG+ P QRF SE+G
Sbjct: 61   AGCPLAATLSEKYSVLVLERGNVPKAHPNTLVASGFLTNLVEADDDDGDTPAQRFTSEEG 120

Query: 121  VENIRGRVLGGGSMVNAGFYSRGHREFFETAGVDWDMELVNKSYQWVEQTVVSRPILNAW 180
            VE++RGRVLGG SM+NA F+S    +F   +GV+WD + V K+Y+WV+  +VS   L+ W
Sbjct: 121  VESVRGRVLGGTSMINAAFFSEADNDFLAKSGVEWDNDEVEKAYEWVKSKIVSYSNLSTW 180

Query: 181  QSAFRTALLEAGVVPDNEFDLKHLVGTKTGGSIFDDKGNRHGAVELLNKAKPENLKVAIQ 240
            QSA + ALLEAGV PDN    KH VGTK  GS FD+ G RHGAVELLNK   +N+K+AI 
Sbjct: 181  QSAVKEALLEAGVGPDNGVTTKHKVGTKQSGSTFDNMGRRHGAVELLNKGNLKNMKIAIH 240

Query: 241  ATVQKIIF----SGLSASGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGIGP 300
            A V+KIIF    S  SA GV Y+DSKGK H A IR KGE+ILSAGA+GSPQLLLLSGIGP
Sbjct: 241  AYVKKIIFSTKSSNPSAIGVIYTDSKGKYHKALIRNKGEVILSAGALGSPQLLLLSGIGP 300

Query: 301  KSHLSSLKLPVVLHQPHVGESMSDNPRFGANIMLPFQLVASSVKVVGTLKNN-IHLQAFA 360
            KS+LSS  +P+V  Q +VG+ M+DNPR   N+++PF    S  +VVG   +  I   +++
Sbjct: 301  KSYLSSHHIPIVHSQSNVGKFMADNPRNNINLIIPFPFEGSGAQVVGITDDYFIETVSYS 360

Query: 361  SP-SPFLVPPSF---SLLPPQSTSIIPSLALFVGKFSQVLSEGSLRLNSS-NVKKSPIVR 420
             P +P  +P SF    L PPQ      SLA  V K    LS GSL L S+ +VK +P VR
Sbjct: 361  LPFAPTTLPFSFYPNPLTPPQF-----SLASIVEKIVGPLSTGSLTLASTKDVKITPHVR 420

Query: 421  FNYYSHPDDLAKCVRGVRKVGDLLQTQTMEKIKTQDLEGNKVFQFLGLPWPENLSDDSSV 480
            FNY+S+P DLA+CV  +RKVG++L+T+++++ K +DL G + F +LG P P N SD+S++
Sbjct: 421  FNYFSNPIDLARCVSAMRKVGEMLETKSLDRFKYKDLNGARDFMYLGPPLPANQSDNSAM 480

Query: 481  EEYCQKTVTTYWHYHGGCLVGKVVDGNFKVIGIENLRVVDGSTFSDSPGTNPMATLMMLG 540
            E+YC+ +VTT+WHYHGGCLVGKVVDG+FKVIG  +LRVVDGSTF  SPGTNP ATLMM+G
Sbjct: 481  EDYCRSSVTTFWHYHGGCLVGKVVDGDFKVIGTNSLRVVDGSTFVISPGTNPQATLMMIG 540

Query: 541  RLPLQNTKFSMWDKWRMRVGFDVGKLYTKFVHDASDLPEKEEYEYIIIGGGTTGCPLAAT 600
            R+   N  F +        G D    Y K V++A+D+P +EEY+YI++GGGT GCPLAAT
Sbjct: 541  RVGDNNGPFGLGIAVS---GADYDFSYMKSVYNATDMPLEEEYDYIVVGGGTAGCPLAAT 600

Query: 601  LSSKFSVLLLERGSDPNKYPSVLNEQGLL-NAFVTEDDGENPFQRFISEDGVENIRGRVL 660
            LS K+SVL+LERG+ P  +P+ L   G L N    +DDG+ P QRF SE GVEN+RGRVL
Sbjct: 601  LSEKYSVLVLERGNVPKAHPNTLVASGFLTNLIEADDDGDTPAQRFTSE-GVENVRGRVL 660

Query: 661  GGGSMVNAGFYSRGHREFFETAGVDWDMELVDKSYQWVEQTVVSRPILNAWQSVFRSALL 720
            GG SM+NA F+S    +F   +GV WD + V+K+Y+WV +++VS   ++ WQ   + ALL
Sbjct: 661  GGTSMINAAFFSEADNDFMVKSGVKWDNDEVEKAYEWVRESIVSFSNMSTWQFAVKEALL 720

Query: 721  EAGVVPDNGFDLRHLVGTKIGGSIFDDKGNRHGVVELLNKAELENLKL------------ 780
            EAGV PDNG   +H +GTK  GS FD+ G RHG VELLNK +L+N+K+            
Sbjct: 721  EAGVGPDNGVTTKHKIGTKQSGSTFDNMGRRHGAVELLNKGDLQNMKIAIHAYVNKVIFS 780

Query: 781  ----------------------HTAFIRKKGEIILSAGAIGSPQLLLLSGIGPKSHLSSL 840
                                  H A IR KGE+ILSAGA+GSPQLLLLSGIGPKS+LSS 
Sbjct: 781  TKSSSSNPSAIGVIYTDSKGKSHKALIRNKGEVILSAGALGSPQLLLLSGIGPKSYLSSQ 840

Query: 841  KLPVVLHQPHVGESMSDNPRFGANIMLPFQLVASSVKVVGTLKNNIHLQAFASPSPFL-V 900
             +P++  Q +VG+ M+DNPR   N+++PF L  S+V+VVG + N+  ++  +   PF   
Sbjct: 841  HIPIIHSQSNVGQFMADNPRNNINLIIPFPLEGSAVQVVG-ITNDYFIETVSYSLPFAPT 900

Query: 901  PPSFSLLPPQSTSIIPSLALFVGKFSQVLSEGSLRLNSS-NVKKSPIVRFNYYSHPDDLP 960
               FS  P   T    SLA    K    LS GSL L S+ +VK +P VRFNY+S+P DL 
Sbjct: 901  TLPFSFYPNPLTQPQFSLATIAAKIVGPLSTGSLTLASTKDVKITPHVRFNYFSNPIDLA 960

Query: 961  KCVRGVRKVGDLLQTQTMVKIKTQDLEGNKVFQFLGPPLPENL-SDDITVENYCKKTVTT 1020
            +CV  +RK+G++L+T+++ + K +DL G + F FLGP LP N  S++  +E+YC+ +VTT
Sbjct: 961  RCVSAMRKLGEMLETKSLDRFKYKDLNGARDFMFLGPTLPSNYQSNNSGMEDYCRSSVTT 1020

Query: 1021 YWHYHGGCLIGKVVDGNYRVIGIENLRVVDGSTFSESPGTNPMATLMMLGRYV 1023
             WHYHGGCL+GKVVDG+++VIG  +LRVVDGSTF  SPGTNP ATLMM+GRY+
Sbjct: 1021 IWHYHGGCLVGKVVDGDFKVIGTNSLRVVDGSTFVISPGTNPQATLMMIGRYL 1062

BLAST of Tan0003508 vs. NCBI nr
Match: KAF4383869.1 (hypothetical protein G4B88_016302, partial [Cannabis sativa])

HSP 1 Score: 1026.5 bits (2653), Expect = 1.5e-295
Identity = 548/1046 (52.39%), Postives = 704/1046 (67.30%), Query Frame = 0

Query: 22   LSSHTISDQDVSYMKLVHNASDIP-EKEEYEYIIIGGGTAGCPLAATLSSKFSVLLLERG 81
            L S T +  D  YMK V++A+++  E+E Y+YIIIGGGTAGCPLAATLS  +SVL+LERG
Sbjct: 87   LLSKTDAMVDFRYMKSVYDATEMSLEEEYYDYIIIGGGTAGCPLAATLSENYSVLVLERG 146

Query: 82   SDPNKYPSVLNEQGLLNAFVAEDDGE--NPFQRFISEDGVENIRGRVLGGGSMVNAGFYS 141
            S P   P+VL+  G     + E++     P QRF SEDGVEN RGRVLGG SM+NAGF+S
Sbjct: 147  SVPTANPNVLHLHGFFANLMQEEEETRVTPAQRFTSEDGVENARGRVLGGTSMINAGFFS 206

Query: 142  RGHREFFETAGVDWDMELVNKSYQWVEQTVVSRPILNAWQSAFRTALLEAGVVPDNEFDL 201
            RG   FF   GV W+M+ V K+Y+WVE+++V RP L  WQS+FR ALLE GV PDNEFDL
Sbjct: 207  RGDEGFFSKPGVKWEMDGVEKAYEWVEESIVFRPKLPLWQSSFRDALLEIGVGPDNEFDL 266

Query: 202  KHLVGTKTGGSIFDDKGNRHGAVELLNKAKPENLKVAIQATVQKIIFSGL---------- 261
             H +GTK  GS FD+ G RHGAVELLNK    NLKV + ATV+KIIFS            
Sbjct: 267  NHKLGTKISGSTFDEVGRRHGAVELLNKGNLNNLKVVVHATVEKIIFSTKVSRNYHSGDD 326

Query: 262  ----------SASGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGIGPKSHLS 321
                       A GV YSDSKGK HT  ++ KGE+ILSAGAIGSPQLLLLSGIGP S+LS
Sbjct: 327  NDHDNNPPKPYAIGVIYSDSKGKSHTVLVKHKGEVILSAGAIGSPQLLLLSGIGPHSYLS 386

Query: 322  SLKLPVVLHQPHVGESMSDNPRFGANIMLPFQLVASSVKVVGTLKNNIHLQAFASPSP-F 381
            SL +P+V  QP+VG+ ++DNPR   N+++P     S V+VVG + N+  ++  ++  P  
Sbjct: 387  SLNIPIVHSQPNVGDFIADNPRNNINLIIPSPTDPSPVQVVG-ITNHYFIETISANLPAS 446

Query: 382  LVPPSFSLLPPQSTSIIPSLALFVGKFSQVLSEGSLRLNSSN-VKKSPIVRFNYYSHPDD 441
            L P  FS+ P  ST+ +  + +   K  Q LS GSL L S N V+ +P VRFNY+SHP D
Sbjct: 447  LTPLPFSVYPKSSTAHL-GMTVICEKLRQPLSSGSLWLASPNDVRVTPHVRFNYFSHPKD 506

Query: 442  LAKCVRGVRKVGDLLQTQTMEKIKTQDLEGNKVFQFLGLPWPENLSDDSSVEEYCQKTVT 501
            L++CV GVRK+G++L+T  ME+ K  D    + F + G   P N SD+S++EE+C+ +VT
Sbjct: 507  LSQCVNGVRKIGEMLETNAMERFKMVDSNEERNFMYFGPSLPTNQSDESAMEEFCRSSVT 566

Query: 502  TYWHYHGGCLVGKVVDGNFKVIGIENLRVVDGSTFSDSPGTNPMATLMMLGRL----PLQ 561
            T WHYHGGC VGKVVDG+F+V+G+ +LRVVDGSTF  SPGTNP AT+MMLGR      LQ
Sbjct: 567  TIWHYHGGCTVGKVVDGDFRVMGVNSLRVVDGSTFRVSPGTNPQATVMMLGRYVGLKMLQ 626

Query: 562  NTKFSMWDKWRMRVGFDVGKLYTKFVHDASDLP-EKEEYEYIIIGGGTTGCPLAATLSSK 621
              +           G D    Y K V+DA+++  E+E Y+YIIIGGGT GCPLAATLS  
Sbjct: 627  EREAEANVLALATSGSDHDFRYMKSVYDATEMSLEEEYYDYIIIGGGTAGCPLAATLSEN 686

Query: 622  FSVLLLERGSDPNKYPSVLNEQGLLNAFVTEDDGE--NPFQRFISEDGVENIRGRVLGGG 681
            +SVL+LERGS P   P+VL+  G L   + E++     P QRF SEDGVEN+RGRVLGG 
Sbjct: 687  YSVLVLERGSVPTSNPNVLHLSGFLANLMQEEEETRVTPAQRFTSEDGVENVRGRVLGGS 746

Query: 682  SMVNAGFYSRGHREFFETAGVDWDMELVDKSYQWVEQTVVSRPILNAWQSVFRSALLEAG 741
            SM+NAGF+SRG   F+  +GV W+M+ V+K+Y+WVE+++V RP L  WQS FR ALLE G
Sbjct: 747  SMINAGFFSRGDEGFYSKSGVKWEMDRVEKAYEWVEESIVFRPKLPVWQSSFRDALLEVG 806

Query: 742  VVPDNGFDLRHLVGTKIGGSIFDDKGNRHGVVELLNKAELENL--------------KLH 801
            VVP+NGFDL H VGTKI GS FD+ G RHG     + ++  +               K H
Sbjct: 807  VVPNNGFDLNHKVGTKISGSTFDEVGRRHGNYHSGDDSDQNSPKPYAIGVIYSDSKGKSH 866

Query: 802  TAFIRKKGEIILSAGAIGSPQLLLLSGIGPKSHLSSLKLPVVLHQPHVGESMSDNPRFGA 861
            T  ++ KGE+ILSAGAIGSPQLLLLSGIGP S+LSSL +P+VL QP+VG+ ++DNPR   
Sbjct: 867  TVLVKHKGEVILSAGAIGSPQLLLLSGIGPHSYLSSLNIPIVLSQPNVGDFIADNPRNNI 926

Query: 862  NIMLPFQLVASSVKVVGTLKNNIHLQAFASPSPFLVPPSFSLLPPQSTSIIPSLALFVGK 921
            N+++PF    S+V+VVG   +       A+      P  FS+ P  ST+ +  +A+   K
Sbjct: 927  NLIIPFPTEPSAVQVVGITDHYFMETISANLPNSPTPLPFSMYPKSSTANL-GMAIICEK 986

Query: 922  FSQVLSEGSLRLNSSN-VKKSPIVRFNYYSHPDDLPKCVRGVRKVGDLLQTQTMVKIKTQ 981
                LS GSL L SSN V+ +P VRFNY+SHP DL +CV  VRK+G++L +++M + K  
Sbjct: 987  LRHPLSSGSLWLASSNDVRVTPHVRFNYFSHPKDLSQCVSAVRKIGEVLSSKSMERFKMV 1046

Query: 982  DLEGNKVFQFLGPPLPENLSDDITVENYCKKTVTTYWHYHGGCLIGKVVDGNYRVIGIEN 1021
            D    + F + GP LP N SD+  +E +C+ +VTT WHYHGGC +GKVVDG++RV G+ +
Sbjct: 1047 DSNEERNFMYFGPSLPTNQSDESAMEEFCRSSVTTIWHYHGGCTVGKVVDGDFRVTGVNS 1106

BLAST of Tan0003508 vs. ExPASy TrEMBL
Match: A0A7J6FBM4 ((R)-mandelonitrile lyase OS=Cannabis sativa OX=3483 GN=G4B88_001010 PE=3 SV=1)

HSP 1 Score: 1044.3 bits (2699), Expect = 3.5e-301
Identity = 557/1064 (52.35%), Postives = 710/1064 (66.73%), Query Frame = 0

Query: 28   SDQDVSYMKLVHNASDIP-EKEEYEYIIIGGGTAGCPLAATLSSKFSVLLLERGSDPNKY 87
            SD D  YMK VH+A+++  E+E Y+YIIIGGGTAGCPLAATLS  +SVL+LERGS P   
Sbjct: 36   SDHDFRYMKSVHDATEMSLEEEYYDYIIIGGGTAGCPLAATLSENYSVLVLERGSVPTAN 95

Query: 88   PSVLNEQGLLNAFVAEDDGE--NPFQRFISEDGVENIRGRVLGGGSMVNAGFYSRGHREF 147
            P+VL+  G     + E++     P QRF SEDGVEN RGRVLGG SM+NAGF+SRG   F
Sbjct: 96   PNVLHLHGFFANLMQEEEETRVTPAQRFTSEDGVENARGRVLGGTSMINAGFFSRGDEGF 155

Query: 148  FETAGVDWDMELVNKSYQWVEQTVVSRPILNAWQSAFRTALLEAGVVPDNEFDLKHLVGT 207
            F   GV W+M+ V K+Y+WVE+++V RP L  WQS+FR ALLE GV PDNEFDL H +GT
Sbjct: 156  FSKPGVKWEMDRVEKAYEWVEESIVFRPKLPLWQSSFRDALLEIGVGPDNEFDLNHKLGT 215

Query: 208  KTGGSIFDDKGNRHGAVELLNKAKPENLKVAIQATVQKIIFSGL---------------- 267
            K  GS FD+ G RHGAVELLNK    NLKV + ATV+KI FS                  
Sbjct: 216  KISGSTFDEVGRRHGAVELLNKGNLNNLKVVVHATVEKIFFSTKVSRNYHSGDDNDHDNN 275

Query: 268  ----SASGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGIGPKSHLSSLKLPV 327
                 A GV YSDSKGK HT  ++ KGE+ILSAGAIGSPQLLLLSGIGP S+LSSL +P+
Sbjct: 276  PPKPYAIGVIYSDSKGKSHTVLVKHKGEVILSAGAIGSPQLLLLSGIGPHSYLSSLNIPI 335

Query: 328  VLHQPHVGESMSDNPRFGANIMLPFQLVASSVKVVGTLKNNIHLQAFASPSP-FLVPPSF 387
            V  QP+VG+ ++DNPR   N+++P     S V+VVG + N+  ++  ++  P  L P  F
Sbjct: 336  VHSQPNVGDFIADNPRNNINLIIPSPTDPSPVQVVG-ITNHYFIETISANLPASLTPLPF 395

Query: 388  SLLPPQSTSIIPSLALFVGKFSQVLSEGSLRLNSSN-VKKSPIVRFNYYSHPDDLAKCVR 447
            S+ P  ST+ +  + +   K  Q LS GSL L S N V+ +P VRFNY+SHP DL++CV 
Sbjct: 396  SVYPKSSTAHL-GMTVICEKLRQPLSSGSLWLASPNDVRVTPHVRFNYFSHPKDLSQCVN 455

Query: 448  GVRKVGDLLQTQTMEKIKTQDLEGNKVFQFLGLPWPENLSDDSSVEEYCQKTVTTYWHYH 507
            GVRK+G++L+T  ME+ K  D    + F + G   P N SD+S++EE+C+ +VTT WHYH
Sbjct: 456  GVRKIGEMLETNAMERFKMVDSNEERNFMYFGPSLPTNQSDESAMEEFCRSSVTTIWHYH 515

Query: 508  GGCLVGKVVDGNFKVIGIENLRVVDGSTFSDSPGTNPMATLMMLGRLPLQNTKFSMWDKW 567
            GGC VGKVVDG+F+V+G+ +LRVVDGSTF  SPGTNP AT+MMLGR         M  + 
Sbjct: 516  GGCTVGKVVDGDFRVMGVNSLRVVDGSTFRVSPGTNPQATVMMLGRY----VGLKMLQER 575

Query: 568  RMRVGFDVGKLYTKFVHDASDLP-EKEEYEYIIIGGGTTGCPLAATLSSKFSVLLLERGS 627
                  +    Y K V+DA+++  E+E Y+YIIIG    GCPLAATLS  +SVL+LERGS
Sbjct: 576  EAEANVE----YMKSVYDATEMSLEEEYYDYIIIG---AGCPLAATLSENYSVLVLERGS 635

Query: 628  DPNKYPSVLNEQGLLNAFVTEDDGE--NPFQRFISEDGVENIRGRVLGGGSMVNAGFYSR 687
             P   P+VL+  G L   + E++     P QRF SEDGVEN+RGRVLGG SM+NAGF+SR
Sbjct: 636  VPTSNPNVLHLSGFLANLMQEEEETRVTPAQRFTSEDGVENVRGRVLGGSSMINAGFFSR 695

Query: 688  GHREFFETAGVDWDMELVDKSYQWVEQTVVSRPILNAWQSVFRSALLEAGVVPDNGFDLR 747
            G   F+  +GV W+M+ V+K+Y+WVE+++V RP L  WQS FR ALLE GV P+NGFDL 
Sbjct: 696  GDEGFYSKSGVKWEMDRVEKAYEWVEESIVFRPKLPVWQSSFRDALLEVGVGPNNGFDLN 755

Query: 748  HLVGTKIGGSIFDDKGNRHGVVELLNKAELENLKL------------------------- 807
            H +GTKI GS FD+ G RHG VELLNK  L NLK+                         
Sbjct: 756  HKLGTKISGSTFDEVGRRHGAVELLNKGNLNNLKVGVHATVDRIIFSTKVSNNYHSGDDS 815

Query: 808  --------HTAFIRKKGEIILSAGAIGSPQLLLLSGIGPKSHLSSLKLPVVLHQPHVGES 867
                    HT  +R KGEIILSAGAIGSPQLLLLSGIGP S+LSSL +P+VL QP+VG+ 
Sbjct: 816  DQNSPRKSHTVLVRHKGEIILSAGAIGSPQLLLLSGIGPHSYLSSLNIPIVLSQPNVGDF 875

Query: 868  MSDNPRFGANIMLPFQLVASSVKVVGTLKNNIHLQAFASPSPFLVPPSFSLLPPQSTSII 927
            ++DNPR   N+++PF    S+V+VVG   +       A+      P  FS+ P  ST+ +
Sbjct: 876  IADNPRNNINLIIPFPTEPSAVQVVGITDHYFMETISANLPNSPTPLPFSMYPKSSTANL 935

Query: 928  PSLALFVGKFSQVLSEGSLRLNSSN-VKKSPIVRFNYYSHPDDLPKCVRGVRKVGDLLQT 987
              +A+   K    LS GSL L SSN V+ +P VRFNY+SHP DL +CV  VRK+G++L +
Sbjct: 936  -GMAIICEKLRHPLSSGSLWLASSNDVRVTPHVRFNYFSHPKDLSQCVSAVRKIGEVLSS 995

Query: 988  QTMVKIKTQDLEGNKVFQFLGPPLPENLSDDITVENYCKKTVTTYWHYHGGCLIGKVVDG 1030
            ++M + K  D    + F + GP LP N SD+  +E +C+ +VTT WHYHGGC +GKVVDG
Sbjct: 996  KSMERFKMVDSNEERNFMYFGPSLPTNQSDESAMEEFCRSSVTTIWHYHGGCTVGKVVDG 1055

BLAST of Tan0003508 vs. ExPASy TrEMBL
Match: A0A7J6DSU4 ((R)-mandelonitrile lyase OS=Cannabis sativa OX=3483 GN=F8388_026317 PE=3 SV=1)

HSP 1 Score: 1039.3 bits (2686), Expect = 1.1e-299
Identity = 565/1125 (50.22%), Postives = 724/1125 (64.36%), Query Frame = 0

Query: 28   SDQDVSYMKLVHNASDIP-EKEEYEYIIIGGGTAGCPLAATLSSKFSVLLLERGSDPNKY 87
            SD D  YMK VH+A+++  E+E Y+YIIIGGGTAGCPLAATLS  +SVL+LERGS P   
Sbjct: 36   SDHDFRYMKSVHDATEMSLEEEYYDYIIIGGGTAGCPLAATLSENYSVLVLERGSVPTAN 95

Query: 88   PSVLNEQGLLNAFVAEDDGE--NPFQRFISEDGVENIRGRVLGGGSMVNAGFYSRGHREF 147
            P+VL+  G     + E++     P QRF SEDGVEN RGRVLGG SM+NAGF+SRG   F
Sbjct: 96   PNVLHLHGFFANLMQEEEETRVTPAQRFTSEDGVENARGRVLGGTSMINAGFFSRGDEGF 155

Query: 148  FETAGVDWDMELVNKSYQWVEQTVVSRPILNAWQSAFRTALLEAGVVPDNEFDLKHLVGT 207
            F   GV W+M+ V K+Y+WVE+++V RP L  WQS+FR ALLE GV PDNEFDL H +GT
Sbjct: 156  FSKPGVKWEMDRVEKAYEWVEESIVFRPKLPLWQSSFRDALLEIGVGPDNEFDLNHKLGT 215

Query: 208  KTGGSIFDDKGNRHGAVELLNKAKPENLKVAIQATVQKIIFSGL---------------- 267
            K  GS FD+ G RHGAVELLNK    NLKV + ATV+KI FS                  
Sbjct: 216  KISGSTFDEVGRRHGAVELLNKGNLNNLKVVVHATVEKIFFSTKVSRNYHSGDDNDHDNN 275

Query: 268  ----SASGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGIGPKSHLSSLKLPV 327
                 A GV YSDSKGK HT  ++ KGE+ILSAGAIGSPQLLLLSGIGP S+LSSL +P+
Sbjct: 276  PPKPYAIGVIYSDSKGKSHTVLVKHKGEVILSAGAIGSPQLLLLSGIGPHSYLSSLNIPI 335

Query: 328  VLHQPHVGESMSDNPRFGANIMLPFQLVASSVKVVGTLKNNIHLQAFASPSP-FLVPPSF 387
            V  QP+VG+ ++DNPR   N+++P     S V+VVG + N+  ++  ++  P  L P  F
Sbjct: 336  VHSQPNVGDFIADNPRNNINLIIPSPTDPSPVQVVG-ITNHYFIETISANLPASLTPLPF 395

Query: 388  SLLPPQSTSIIPSLALFVGKFSQVLSEGSLRLNSSN-VKKSPIVRFNYYSHPDDLAKCVR 447
            S+ P  ST+ +  + +   K  Q LS GSL L S N V+ +P VRFNY+SHP DL++CV 
Sbjct: 396  SVYPKSSTAHL-GMTVICEKLRQPLSSGSLWLASPNDVRVTPHVRFNYFSHPKDLSQCVN 455

Query: 448  GVRKVGDLLQTQTMEKIKTQDLEGNKVFQFLGLPWPENLSDDSSVEEYCQKTVTTYWHYH 507
            GVRK+G++L+T  ME+ K  D    + F + G   P N SD+S++EE+C+ +VTT WHYH
Sbjct: 456  GVRKIGEMLETNAMERFKMVDSNEERNFMYFGPSLPTNQSDESAMEEFCRSSVTTIWHYH 515

Query: 508  GGCLVGKVVDGNFKVIGIENLRVVDGSTFSDSPGTNPMATLMMLGR-------------- 567
            GGC VGKVVDG+F+V+G+ +LRVVDGSTF  SPGTNP AT+MMLGR              
Sbjct: 516  GGCTVGKVVDGDFRVMGVNSLRVVDGSTFRVSPGTNPQATVMMLGRYVGLKMLQEREAEA 575

Query: 568  -----------------------LPLQNTKFSMWDKWRMRV-----GFDVGKLYTKFVHD 627
                                   + L+ ++++  D  +  +     G D    Y K V+D
Sbjct: 576  NVEGDIERREIDEKDRVKDYLSTVALRVSRWATSDGQQQVLALATSGSDHDFRYMKSVYD 635

Query: 628  ASDLP-EKEEYEYIIIGGG--TTGCPLAATLSSKFSVLLLERGSDPNKYPSVLNEQGLLN 687
            A+++  E+E Y+YIIIGGG  T GCPLAATLS  +SVL+LERGS P   P+VL+  G L 
Sbjct: 636  ATEMSLEEEYYDYIIIGGGTLTAGCPLAATLSENYSVLVLERGSVPTSNPNVLHLSGFLA 695

Query: 688  AFVTEDDGE--NPFQRFISEDGVENIRGRVLGGGSMVNAGFYSRGHREFFETAGVDWDME 747
              + E++     P QRF SEDGVEN+RGRVLGG SM+NAGF+SRG   F+  +GV W+M+
Sbjct: 696  NLMQEEEETRVTPAQRFTSEDGVENVRGRVLGGSSMINAGFFSRGDEGFYSKSGVKWEMD 755

Query: 748  LVDKSYQWVEQTVVSRPILNAWQSVFRSALLEAGVVPDNGFDLRHLVGTKIGGSIFDDKG 807
             V+K+Y+WVE+++V RP L  WQS FR ALLE GV P+NGFDL H +GTKI GS FD+ G
Sbjct: 756  RVEKAYEWVEESIVFRPKLPVWQSSFRDALLEVGVGPNNGFDLNHKLGTKISGSTFDEVG 815

Query: 808  NRHGVVELLNKAELENLKL----------------------------------------- 867
             RHG VELLNK  L NLK+                                         
Sbjct: 816  RRHGAVELLNKGNLNNLKVGVHATVDRIIFSTKVSNNYHSGDDSDQNSPKPYAIGVMYSD 875

Query: 868  -----HTAFIRKKGEIILSAGAIGSPQLLLLSGIGPKSHLSSLKLPVVLHQPHVGESMSD 927
                 HT  +R KGEIILSAGAIGSPQLLLLSGIGP S+LSSL +P+VL QP+VG+ ++D
Sbjct: 876  SKGKSHTVLVRHKGEIILSAGAIGSPQLLLLSGIGPHSYLSSLNIPIVLSQPNVGDFIAD 935

Query: 928  NPRFGANIMLPFQLVASSVKVVGTLKNNIHLQAFASPSPFLVPPSFSLLPPQSTSIIPSL 987
            NPR   N+++PF    S+V+VVG   +       A+      P  FS+ P  ST+ +  +
Sbjct: 936  NPRNNINLIIPFPTEPSAVQVVGITDHYFMETISANLPNSPTPLPFSMYPKSSTANL-GM 995

Query: 988  ALFVGKFSQVLSEGSLRLNSSN-VKKSPIVRFNYYSHPDDLPKCVRGVRKVGDLLQTQTM 1034
            A+   K    LS GSL L SSN V+ +P VRFNY+SHP DL +CV  VRK+G++L +++M
Sbjct: 996  AIICEKLRHPLSSGSLWLASSNDVRVTPHVRFNYFSHPKDLSQCVSAVRKIGEVLSSKSM 1055

BLAST of Tan0003508 vs. ExPASy TrEMBL
Match: A0A7J6GLR3 ((R)-mandelonitrile lyase OS=Cannabis sativa OX=3483 GN=G4B88_016288 PE=3 SV=1)

HSP 1 Score: 1036.9 bits (2680), Expect = 5.5e-299
Identity = 559/1073 (52.10%), Postives = 733/1073 (68.31%), Query Frame = 0

Query: 1    MEHSMATIL-LLILISLFQFGVLSSHTISDQDVSYMKLVHNASDIPEKEEYEYIIIGGGT 60
            ME S  +IL LLI ++ F    L   + +D D SYMK V+NA+D+P +EEY+YI++GGGT
Sbjct: 1    MESSSLSILTLLIFLTCFDHAKLLV-SGADYDFSYMKSVYNATDMPLEEEYDYIVVGGGT 60

Query: 61   AGCPLAATLSSKFSVLLLERGSDPNKYPSVLNEQGLLNAFVA--EDDGENPFQRFISEDG 120
            AGCPLAATLS K+SVL+LERG+ P  +P+ L   G L   V   +DDG+ P QRF SE+G
Sbjct: 61   AGCPLAATLSEKYSVLVLERGNVPKAHPNTLVASGFLTNLVEADDDDGDTPAQRFTSEEG 120

Query: 121  VENIRGRVLGGGSMVNAGFYSRGHREFFETAGVDWDMELVNKSYQWVEQTVVSRPILNAW 180
            VE++RGRVLGG SM+NA F+S    +F   +GV+WD + V K+Y+WV+  +VS   L+ W
Sbjct: 121  VESVRGRVLGGTSMINAAFFSEADNDFLAKSGVEWDNDEVEKAYEWVKSKIVSYSNLSTW 180

Query: 181  QSAFRTALLEAGVVPDNEFDLKHLVGTKTGGSIFDDKGNRHGAVELLNKAKPENLKVAIQ 240
            QSA + ALLEAGV PDN    KH VGTK  GS FD+ G RHGAVELLNK   +N+K+AI 
Sbjct: 181  QSAVKEALLEAGVGPDNGVTTKHKVGTKQSGSTFDNMGRRHGAVELLNKGNLKNMKIAIH 240

Query: 241  ATVQKIIF----SGLSASGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGIGP 300
            A V+KIIF    S  SA GV Y+DSKGK H A IR KGE+ILSAGA+GSPQLLLLSGIGP
Sbjct: 241  AYVKKIIFSTKSSNPSAIGVIYTDSKGKYHKALIRNKGEVILSAGALGSPQLLLLSGIGP 300

Query: 301  KSHLSSLKLPVVLHQPHVGESMSDNPRFGANIMLPFQLVASSVKVVGTLKNN-IHLQAFA 360
            KS+LSS  +P+V  Q +VG+ M+DNPR   N+++PF    S  +VVG   +  I   +++
Sbjct: 301  KSYLSSHHIPIVHSQSNVGKFMADNPRNNINLIIPFPFEGSGAQVVGITDDYFIETVSYS 360

Query: 361  SP-SPFLVPPSF---SLLPPQSTSIIPSLALFVGKFSQVLSEGSLRLNSS-NVKKSPIVR 420
             P +P  +P SF    L PPQ      SLA  V K    LS GSL L S+ +VK +P VR
Sbjct: 361  LPFAPTTLPFSFYPNPLTPPQF-----SLASIVEKIVGPLSTGSLTLASTKDVKITPHVR 420

Query: 421  FNYYSHPDDLAKCVRGVRKVGDLLQTQTMEKIKTQDLEGNKVFQFLGLPWPENLSDDSSV 480
            FNY+S+P DLA+CV  +RKVG++L+T+++++ K +DL G + F +LG P P N SD+S++
Sbjct: 421  FNYFSNPIDLARCVSAMRKVGEMLETKSLDRFKYKDLNGARDFMYLGPPLPANQSDNSAM 480

Query: 481  EEYCQKTVTTYWHYHGGCLVGKVVDGNFKVIGIENLRVVDGSTFSDSPGTNPMATLMMLG 540
            E+YC+ +VTT+WHYHGGCLVGKVVDG+FKVIG  +LRVVDGSTF  SPGTNP ATLMM+G
Sbjct: 481  EDYCRSSVTTFWHYHGGCLVGKVVDGDFKVIGTNSLRVVDGSTFVISPGTNPQATLMMIG 540

Query: 541  RLPLQNTKFSMWDKWRMRVGFDVGKLYTKFVHDASDLPEKEEYEYIIIGGGTTGCPLAAT 600
            R+   N  F +        G D    Y K V++A+D+P +EEY+YI++GGGT GCPLAAT
Sbjct: 541  RVGDNNGPFGLGIAVS---GADYDFSYMKSVYNATDMPLEEEYDYIVVGGGTAGCPLAAT 600

Query: 601  LSSKFSVLLLERGSDPNKYPSVLNEQGLL-NAFVTEDDGENPFQRFISEDGVENIRGRVL 660
            LS K+SVL+LERG+ P  +P+ L   G L N    +DDG+ P QRF SE GVEN+RGRVL
Sbjct: 601  LSEKYSVLVLERGNVPKAHPNTLVASGFLTNLIEADDDGDTPAQRFTSE-GVENVRGRVL 660

Query: 661  GGGSMVNAGFYSRGHREFFETAGVDWDMELVDKSYQWVEQTVVSRPILNAWQSVFRSALL 720
            GG SM+NA F+S    +F   +GV WD + V+K+Y+WV +++VS   ++ WQ   + ALL
Sbjct: 661  GGTSMINAAFFSEADNDFMVKSGVKWDNDEVEKAYEWVRESIVSFSNMSTWQFAVKEALL 720

Query: 721  EAGVVPDNGFDLRHLVGTKIGGSIFDDKGNRHGVVELLNKAELENLKL------------ 780
            EAGV PDNG   +H +GTK  GS FD+ G RHG VELLNK +L+N+K+            
Sbjct: 721  EAGVGPDNGVTTKHKIGTKQSGSTFDNMGRRHGAVELLNKGDLQNMKIAIHAYVNKVIFS 780

Query: 781  ----------------------HTAFIRKKGEIILSAGAIGSPQLLLLSGIGPKSHLSSL 840
                                  H A IR KGE+ILSAGA+GSPQLLLLSGIGPKS+LSS 
Sbjct: 781  TKSSSSNPSAIGVIYTDSKGKSHKALIRNKGEVILSAGALGSPQLLLLSGIGPKSYLSSQ 840

Query: 841  KLPVVLHQPHVGESMSDNPRFGANIMLPFQLVASSVKVVGTLKNNIHLQAFASPSPFL-V 900
             +P++  Q +VG+ M+DNPR   N+++PF L  S+V+VVG + N+  ++  +   PF   
Sbjct: 841  HIPIIHSQSNVGQFMADNPRNNINLIIPFPLEGSAVQVVG-ITNDYFIETVSYSLPFAPT 900

Query: 901  PPSFSLLPPQSTSIIPSLALFVGKFSQVLSEGSLRLNSS-NVKKSPIVRFNYYSHPDDLP 960
               FS  P   T    SLA    K    LS GSL L S+ +VK +P VRFNY+S+P DL 
Sbjct: 901  TLPFSFYPNPLTQPQFSLATIAAKIVGPLSTGSLTLASTKDVKITPHVRFNYFSNPIDLA 960

Query: 961  KCVRGVRKVGDLLQTQTMVKIKTQDLEGNKVFQFLGPPLPENL-SDDITVENYCKKTVTT 1020
            +CV  +RK+G++L+T+++ + K +DL G + F FLGP LP N  S++  +E+YC+ +VTT
Sbjct: 961  RCVSAMRKLGEMLETKSLDRFKYKDLNGARDFMFLGPTLPSNYQSNNSGMEDYCRSSVTT 1020

Query: 1021 YWHYHGGCLIGKVVDGNYRVIGIENLRVVDGSTFSESPGTNPMATLMMLGRYV 1023
             WHYHGGCL+GKVVDG+++VIG  +LRVVDGSTF  SPGTNP ATLMM+GRY+
Sbjct: 1021 IWHYHGGCLVGKVVDGDFKVIGTNSLRVVDGSTFVISPGTNPQATLMMIGRYL 1062

BLAST of Tan0003508 vs. ExPASy TrEMBL
Match: A0A7J6GP35 ((R)-mandelonitrile lyase OS=Cannabis sativa OX=3483 GN=G4B88_016302 PE=3 SV=1)

HSP 1 Score: 1026.5 bits (2653), Expect = 7.5e-296
Identity = 548/1046 (52.39%), Postives = 704/1046 (67.30%), Query Frame = 0

Query: 22   LSSHTISDQDVSYMKLVHNASDIP-EKEEYEYIIIGGGTAGCPLAATLSSKFSVLLLERG 81
            L S T +  D  YMK V++A+++  E+E Y+YIIIGGGTAGCPLAATLS  +SVL+LERG
Sbjct: 87   LLSKTDAMVDFRYMKSVYDATEMSLEEEYYDYIIIGGGTAGCPLAATLSENYSVLVLERG 146

Query: 82   SDPNKYPSVLNEQGLLNAFVAEDDGE--NPFQRFISEDGVENIRGRVLGGGSMVNAGFYS 141
            S P   P+VL+  G     + E++     P QRF SEDGVEN RGRVLGG SM+NAGF+S
Sbjct: 147  SVPTANPNVLHLHGFFANLMQEEEETRVTPAQRFTSEDGVENARGRVLGGTSMINAGFFS 206

Query: 142  RGHREFFETAGVDWDMELVNKSYQWVEQTVVSRPILNAWQSAFRTALLEAGVVPDNEFDL 201
            RG   FF   GV W+M+ V K+Y+WVE+++V RP L  WQS+FR ALLE GV PDNEFDL
Sbjct: 207  RGDEGFFSKPGVKWEMDGVEKAYEWVEESIVFRPKLPLWQSSFRDALLEIGVGPDNEFDL 266

Query: 202  KHLVGTKTGGSIFDDKGNRHGAVELLNKAKPENLKVAIQATVQKIIFSGL---------- 261
             H +GTK  GS FD+ G RHGAVELLNK    NLKV + ATV+KIIFS            
Sbjct: 267  NHKLGTKISGSTFDEVGRRHGAVELLNKGNLNNLKVVVHATVEKIIFSTKVSRNYHSGDD 326

Query: 262  ----------SASGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGIGPKSHLS 321
                       A GV YSDSKGK HT  ++ KGE+ILSAGAIGSPQLLLLSGIGP S+LS
Sbjct: 327  NDHDNNPPKPYAIGVIYSDSKGKSHTVLVKHKGEVILSAGAIGSPQLLLLSGIGPHSYLS 386

Query: 322  SLKLPVVLHQPHVGESMSDNPRFGANIMLPFQLVASSVKVVGTLKNNIHLQAFASPSP-F 381
            SL +P+V  QP+VG+ ++DNPR   N+++P     S V+VVG + N+  ++  ++  P  
Sbjct: 387  SLNIPIVHSQPNVGDFIADNPRNNINLIIPSPTDPSPVQVVG-ITNHYFIETISANLPAS 446

Query: 382  LVPPSFSLLPPQSTSIIPSLALFVGKFSQVLSEGSLRLNSSN-VKKSPIVRFNYYSHPDD 441
            L P  FS+ P  ST+ +  + +   K  Q LS GSL L S N V+ +P VRFNY+SHP D
Sbjct: 447  LTPLPFSVYPKSSTAHL-GMTVICEKLRQPLSSGSLWLASPNDVRVTPHVRFNYFSHPKD 506

Query: 442  LAKCVRGVRKVGDLLQTQTMEKIKTQDLEGNKVFQFLGLPWPENLSDDSSVEEYCQKTVT 501
            L++CV GVRK+G++L+T  ME+ K  D    + F + G   P N SD+S++EE+C+ +VT
Sbjct: 507  LSQCVNGVRKIGEMLETNAMERFKMVDSNEERNFMYFGPSLPTNQSDESAMEEFCRSSVT 566

Query: 502  TYWHYHGGCLVGKVVDGNFKVIGIENLRVVDGSTFSDSPGTNPMATLMMLGRL----PLQ 561
            T WHYHGGC VGKVVDG+F+V+G+ +LRVVDGSTF  SPGTNP AT+MMLGR      LQ
Sbjct: 567  TIWHYHGGCTVGKVVDGDFRVMGVNSLRVVDGSTFRVSPGTNPQATVMMLGRYVGLKMLQ 626

Query: 562  NTKFSMWDKWRMRVGFDVGKLYTKFVHDASDLP-EKEEYEYIIIGGGTTGCPLAATLSSK 621
              +           G D    Y K V+DA+++  E+E Y+YIIIGGGT GCPLAATLS  
Sbjct: 627  EREAEANVLALATSGSDHDFRYMKSVYDATEMSLEEEYYDYIIIGGGTAGCPLAATLSEN 686

Query: 622  FSVLLLERGSDPNKYPSVLNEQGLLNAFVTEDDGE--NPFQRFISEDGVENIRGRVLGGG 681
            +SVL+LERGS P   P+VL+  G L   + E++     P QRF SEDGVEN+RGRVLGG 
Sbjct: 687  YSVLVLERGSVPTSNPNVLHLSGFLANLMQEEEETRVTPAQRFTSEDGVENVRGRVLGGS 746

Query: 682  SMVNAGFYSRGHREFFETAGVDWDMELVDKSYQWVEQTVVSRPILNAWQSVFRSALLEAG 741
            SM+NAGF+SRG   F+  +GV W+M+ V+K+Y+WVE+++V RP L  WQS FR ALLE G
Sbjct: 747  SMINAGFFSRGDEGFYSKSGVKWEMDRVEKAYEWVEESIVFRPKLPVWQSSFRDALLEVG 806

Query: 742  VVPDNGFDLRHLVGTKIGGSIFDDKGNRHGVVELLNKAELENL--------------KLH 801
            VVP+NGFDL H VGTKI GS FD+ G RHG     + ++  +               K H
Sbjct: 807  VVPNNGFDLNHKVGTKISGSTFDEVGRRHGNYHSGDDSDQNSPKPYAIGVIYSDSKGKSH 866

Query: 802  TAFIRKKGEIILSAGAIGSPQLLLLSGIGPKSHLSSLKLPVVLHQPHVGESMSDNPRFGA 861
            T  ++ KGE+ILSAGAIGSPQLLLLSGIGP S+LSSL +P+VL QP+VG+ ++DNPR   
Sbjct: 867  TVLVKHKGEVILSAGAIGSPQLLLLSGIGPHSYLSSLNIPIVLSQPNVGDFIADNPRNNI 926

Query: 862  NIMLPFQLVASSVKVVGTLKNNIHLQAFASPSPFLVPPSFSLLPPQSTSIIPSLALFVGK 921
            N+++PF    S+V+VVG   +       A+      P  FS+ P  ST+ +  +A+   K
Sbjct: 927  NLIIPFPTEPSAVQVVGITDHYFMETISANLPNSPTPLPFSMYPKSSTANL-GMAIICEK 986

Query: 922  FSQVLSEGSLRLNSSN-VKKSPIVRFNYYSHPDDLPKCVRGVRKVGDLLQTQTMVKIKTQ 981
                LS GSL L SSN V+ +P VRFNY+SHP DL +CV  VRK+G++L +++M + K  
Sbjct: 987  LRHPLSSGSLWLASSNDVRVTPHVRFNYFSHPKDLSQCVSAVRKIGEVLSSKSMERFKMV 1046

Query: 982  DLEGNKVFQFLGPPLPENLSDDITVENYCKKTVTTYWHYHGGCLIGKVVDGNYRVIGIEN 1021
            D    + F + GP LP N SD+  +E +C+ +VTT WHYHGGC +GKVVDG++RV G+ +
Sbjct: 1047 DSNEERNFMYFGPSLPTNQSDESAMEEFCRSSVTTIWHYHGGCTVGKVVDGDFRVTGVNS 1106

BLAST of Tan0003508 vs. ExPASy TrEMBL
Match: A0A7J6FBQ5 ((R)-mandelonitrile lyase OS=Cannabis sativa OX=3483 GN=G4B88_001041 PE=3 SV=1)

HSP 1 Score: 1019.2 bits (2634), Expect = 1.2e-293
Identity = 537/1032 (52.03%), Postives = 708/1032 (68.60%), Query Frame = 0

Query: 21   VLSSHTISDQDVSYMKLVHNASDIPEKEEYEYIIIGGGTAGCPLAATLSSKFSVLLLERG 80
            +LS      ++ SYMK V+NA D+P  EEY+YI+IGGGTAGCPLAATLS K+S+L+LERG
Sbjct: 441  LLSWKITCSRNFSYMKSVYNAIDLPLVEEYDYIVIGGGTAGCPLAATLSEKYSILVLERG 500

Query: 81   SDPNKYPSVLNEQGLLNAFVAEDDGENPFQRFISEDGVENIRGRVLGGGSMVNAGFYSRG 140
            + P  +P+VL+  G L  F+ ED+G+ P QRF SEDGV+NIRGRVLGG SMVN GF+S  
Sbjct: 501  NTPKAHPNVLSLSGFLKNFIEEDNGDTPAQRFTSEDGVDNIRGRVLGGSSMVNGGFFSEA 560

Query: 141  HREFFETAGVDWDMELVNKSYQWVEQTVVSRPILNAWQSAFRTALLEAGVVPDNEFDLKH 200
              +FF  +GV+WDM  V K+Y+WV+  +VS   L+ WQS  + ALLE G+ PDN+   K+
Sbjct: 561  DDDFFAKSGVEWDMNEVQKAYKWVKNKIVSYSNLSIWQSVVKQALLEVGIGPDNKVTTKY 620

Query: 201  LVGTKTGGSIFDDKGNRHGAVELLNKAKPENLKVAIQATVQKIIFSGLSASGVSYSDSKG 260
             +GTK  GSIFD+ G RHGAVELLNK   +NL++AI A V +           S S  K 
Sbjct: 621  KIGTKQSGSIFDNMGRRHGAVELLNKGNLKNLRIAIHAYVSQ-----------SISTQKR 680

Query: 261  KLHTAFIRKKGEIILSAGAIGSPQLLLLSGIGPKSHLSSLKLPVVLHQPHVGESMSDNPR 320
            +                 A+GSPQLLLLSGIGPKS+LSS  +P+VL QP+VG+ M+DNPR
Sbjct: 681  R----------------SALGSPQLLLLSGIGPKSYLSSQHIPIVLSQPNVGKFMADNPR 740

Query: 321  FGANIMLPFQLVASSVKVVG--------TLKNNIHLQAFASPSPFLVPPSFSL-LPPQST 380
               N++ PF L +S ++VVG        TL  N+       PS F  P ++SL +PP   
Sbjct: 741  NNLNLITPFPLESSILQVVGITKEYYIETLSYNLPFSPTTLPSSFF-PHNYSLAIPPLQL 800

Query: 381  SIIPSLALFVGKFSQVLSEGSLRL-NSSNVKKSPIVRFNYYSHPDDLAKCVRGVRKVGDL 440
            S+    A  VGK +  LS+GSL L ++S+VK +P VRFNY+S+P DL +CV  + K  DL
Sbjct: 801  SV----ANIVGKVAGPLSKGSLWLASNSDVKATPHVRFNYFSNPIDLERCVSMMGKFRDL 860

Query: 441  LQTQTMEKIKTQDLEGNKVFQFLGLPWPENLSDDSSVEEYCQKTVTTYWHYHGGCLVGKV 500
            L+T++++++K  DL+GN+ F F G   P N +  S ++ YC+ +VTT+WHYHGGC VGKV
Sbjct: 861  LKTKSLDRMKYNDLKGNRDFLFYGPSLPTNQTTISVIKHYCRSSVTTFWHYHGGCQVGKV 920

Query: 501  VDGNFKVIGIENLRVVDGSTFSDSPGTNPMATLMMLGRLPLQNTKFSMWDKWRMRVGFDV 560
            VDG+FKVIGI++LRVVDGSTF  SPGTNP A++MM+GR            K R  V    
Sbjct: 921  VDGDFKVIGIDSLRVVDGSTFISSPGTNPQASIMMIGRYLGLKMIRERQPKTRNLVN--- 980

Query: 561  GKLYTKFVHDASDLPEKEEYEYIIIGGGTTGCPLAATLSSKFSVLLLERGSDPNKYPSVL 620
               Y K V++A+DLP  E+Y+YI+IGGGT GCPLAATLS K+SVL+LERG+ P  +P+ L
Sbjct: 981  ---YMKSVYNATDLPLVEKYDYIVIGGGTAGCPLAATLSEKYSVLVLERGNVPKAHPNTL 1040

Query: 621  NEQGLLNAFVTEDDGENPFQRFISEDGVENIRGRVLGGGSMVNAGFYSRGHREFFETAGV 680
               G L   + EDDG  P QRF SEDGVEN+RGRVLGG SM+NA F+S    +F   +GV
Sbjct: 1041 VSSGFLANLIEEDDGTTPAQRFTSEDGVENVRGRVLGGSSMINAAFFSEADNDFLAKSGV 1100

Query: 681  DWDMELVDKSYQWVEQTVVSRPILNAWQSVFRSALLEAGVVPDNGFDLRHLVGTKIGGSI 740
            +WDM+ V+K+Y+WV+  +VS   L+ WQ+  + A +EAG+ PDNG   +H +G K  GS 
Sbjct: 1101 EWDMDAVEKAYEWVKNKIVSYSNLSFWQAAAKEAFVEAGIGPDNGVTTKHKIGAKQSGST 1160

Query: 741  FDDKGNRHGVVELLNKAELENLKL------HTAFIRKKGEIILSAGAIGSPQLLLLSGIG 800
            FDD G RHG VELLN   L NLK+      H A IR KGE+ILSAGA+GSPQLLLLSGIG
Sbjct: 1161 FDDMGRRHGAVELLNNGNLNNLKIAIEASSHKALIRDKGEVILSAGALGSPQLLLLSGIG 1220

Query: 801  PKSHLSSLKLPVVLHQPHVGESMSDNPRFGANIMLPFQLVASSVKVVGTLKN-NIHLQAF 860
            PKS+LSS  +P+VL QP+VG+ M+DNPR   N+++PF L AS+ +VVG  K+  I   ++
Sbjct: 1221 PKSYLSSQNIPIVLSQPNVGKFMADNPRNNLNLIIPFPLEASTGQVVGITKDYYIETNSY 1280

Query: 861  ASP-SPFLVPPSF---SLLPPQSTSIIPSLALFVGKFSQVLSEGSLRL-NSSNVKKSPIV 920
            + P SP  +P  F    LLPPQ T     L +FV K    LS G+L L ++++VK +P V
Sbjct: 1281 SLPFSPTKLPFGFYPTPLLPPQLT-----LTVFVEKIVGPLSTGALTLASATDVKVTPHV 1340

Query: 921  RFNYYSHPDDLPKCVRGVRKVGDLLQTQTMVKIKTQDLEGNKVFQFLGPPLPENLSDDIT 980
            RFNY+S+P DL +CV  ++K G+LL+T++M + K +D+ G + F FLG  LP N SDD  
Sbjct: 1341 RFNYFSNPIDLARCVSAMKKFGELLKTKSMDRFKYKDMNGVRDFMFLGQLLPINQSDDSL 1400

Query: 981  VENYCKKTVTTYWHYHGGCLIGKVVDGNYRVIGIENLRVVDGSTFSESPGTNPMATLMML 1031
            +E+YC+ +VTT+WHYHGGCL+GKVVDG +RVIG  +LRVVDGSTF+ SPGTNP ATLMM+
Sbjct: 1401 MEDYCRSSVTTFWHYHGGCLVGKVVDGEFRVIGANSLRVVDGSTFTVSPGTNPQATLMMI 1429

BLAST of Tan0003508 vs. TAIR 10
Match: AT1G73050.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 507.7 bits (1306), Expect = 2.2e-143
Identity = 257/535 (48.04%), Postives = 363/535 (67.85%), Query Frame = 0

Query: 6   ATILLLILISLFQFGVL---SSHTISDQDVSYMKLVHNASDIPEKEEYEYIIIGGGTAGC 65
           +++L   L+ L   GV+   ++    ++   +M+ + NA+D   ++ Y+YII+GGGTAGC
Sbjct: 7   SSLLYTALVVLLLLGVVHRSNARPRVNRPPGFMRFISNATDFASEDYYDYIIVGGGTAGC 66

Query: 66  PLAATLSSKFSVLLLERGSDPNKYPSVLNEQGLLNAFVAEDDGENPFQRFISEDGVENIR 125
           PLAATLS  F VLLLERG  P   P+V++  G L      ++ ++P Q FISE+GV N R
Sbjct: 67  PLAATLSQSFRVLLLERGGVPYNRPNVMSHDGFLTTLTDVNNFDSPAQSFISEEGVPNAR 126

Query: 126 GRVLGGGSMVNAGFYSRGHREFFETAGVDWDMELVNKSYQWVEQTVVSRPILNAWQSAFR 185
           GRVLGG S +NAGFYSR  ++FFE +G+ WD+  VN+SY+WVE+ +V RP L  WQ+A R
Sbjct: 127 GRVLGGSSAINAGFYSRADKQFFENSGLVWDLSSVNQSYEWVERAIVFRPQLRTWQTAIR 186

Query: 186 TALLEAGVVPDNEFDLKHLVGTKTGGSIFDDKGNRHGAVELLNKAKPENLKVAIQATVQK 245
            ALLE GV P N F L+H VGTK GGS FD  G RH + +LL  A+  N++VA+ ATV++
Sbjct: 187 DALLEVGVHPFNGFTLEHKVGTKIGGSTFDRTGRRHSSADLLRYARSSNIRVAVYATVER 246

Query: 246 IIF--------SGLSASGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGIGPK 305
           ++         S +SA GV Y D  G+ H A IR +GE+ILSAGA+GSPQLL LSGIGP+
Sbjct: 247 VLLASSPSVSGSNVSAIGVVYRDQLGRFHHALIRDRGEVILSAGALGSPQLLFLSGIGPR 306

Query: 306 SHLSSLKLPVVLHQPHVGESMSDNPRFGANIMLPFQLVASSVKVVGTLKNNIHLQAFASP 365
           S+LS+  +PV L QPHVG+ + DNPR G +I+ P  +  S ++VVG  ++   L+A ++ 
Sbjct: 307 SYLSTWGIPVALDQPHVGDFVYDNPRNGISIVPPVPMENSLIQVVGVTEDGAFLEAASNV 366

Query: 366 SPFLVP-PSFSLLPPQSTSIIPSLALFVGKFSQVLSEGSLRLNSSNVKKSPIVRFNYYSH 425
            PF  P  S  +  P S   +P +   + K    +S G LRL S++V+ +P+VRFNY+S 
Sbjct: 367 IPFASPLHSVFIRAPASPLYVP-VTTIMEKILGPVSIGLLRLASTDVRINPVVRFNYFSD 426

Query: 426 PDDLAKCVRGVRKVGDLLQTQTMEKIKTQDLEGNKVFQFLGLPWPENLSDDSSVEEYCQK 485
           P DL +CV G RK+G++L+++ M+    ++  GN+ F+F+G P P + S+D  + ++C++
Sbjct: 427 PQDLERCVNGTRKIGEILRSRAMQDFMIREWFGNRRFRFVGAPLPVDQSNDLVMADFCRR 486

Query: 486 TVTTYWHYHGGCLVGKVVDGNFKVIGIENLRVVDGSTFSDSPGTNPMATLMMLGR 529
           TV+T WHYHGG +VGKVVD + KVIG+ +LR+VDGSTF+ SPGTNP ATLMMLGR
Sbjct: 487 TVSTIWHYHGGAVVGKVVDSDLKVIGVNSLRLVDGSTFNISPGTNPQATLMMLGR 540

BLAST of Tan0003508 vs. TAIR 10
Match: AT1G12570.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 417.9 bits (1073), Expect = 2.3e-116
Identity = 241/557 (43.27%), Postives = 323/557 (57.99%), Query Frame = 0

Query: 10  LLILISLFQFGVLSSHTISDQDVSYMKLVHNASDIPEKEEYEYIIIGGGTAGCPLAATLS 69
           L + +SLF    + S   SD+  +Y   + +A+  P    Y+YIIIGGGTAGCPLAATLS
Sbjct: 10  LFVALSLFLHSPICS---SDKAPNY-SFMRDATGSPTTSYYDYIIIGGGTAGCPLAATLS 69

Query: 70  SKFSVLLLERGSDPNKYPSVLNEQGLLNAFVAEDDGENPFQRFISEDGVENIRGRVLGGG 129
              SVLLLERG  P   P++        A +++    +P QRF+SEDGV N R RVLGGG
Sbjct: 70  QNASVLLLERGDSPYNNPNI-TRLSAFGAALSDLSESSPSQRFVSEDGVINARARVLGGG 129

Query: 130 SMVNAGFYSRGHREFFETAGVDWDMELVNKSYQWVEQTVVSRPILNAWQSAFRTALLEAG 189
           S +NAGFY+R   ++    G  WD  L N+SYQWVE  V  +P +  WQ+A R  LLEAG
Sbjct: 130 SALNAGFYTRAGTKYVRNMG--WDGALANESYQWVEAKVAFQPPMGRWQTAVRDGLLEAG 189

Query: 190 VVPDNEFDLKHLVGTKTGGSIFDDKGNRHGAVELLNKAKPENLKVAIQATVQKIIFSGLS 249
           +VP+N F   H+ GTK GG+IFD  GNRH A +LL  A P+ + V + ATV +I+F    
Sbjct: 190 IVPNNGFTYDHINGTKFGGTIFDRNGNRHTAADLLEYADPKGITVLLHATVHRILFRTRG 249

Query: 250 -----ASGVSYSDSKGKLHTAFIRK--KGEIILSAGAIGSPQLLLLSGIGPKSHLSSLKL 309
                A+GV Y D  G+ H A++++    EIILSAG +GSPQLL+LSG+GP + L +  +
Sbjct: 250 TTKPIANGVVYRDRTGQAHRAYLKEGALSEIILSAGTLGSPQLLMLSGVGPSAQLQAQNI 309

Query: 310 PVVLHQPHVGESMSDNPRFGANIMLPFQLVASSVKVVGTLKNNIHLQAFASPS------- 369
            VV+ QPHVG+ M DNP     +  P  +  S ++VVG      +++A    +       
Sbjct: 310 TVVMDQPHVGQGMYDNPMNAVFVPSPVPVEVSLIEVVGITGEGTYVEAAGGENFGGGGGG 369

Query: 370 ----------PFLVPPSFSLLPPQSTSIIPSLALFVG-----KFSQVLSEGSLRLNSSNV 429
                       +  P  +LL   S + + S   F G     K    LS G L L + N 
Sbjct: 370 SSGSSSTRDYYAMFSPRATLLESNSMTKLSSAQPFQGGFLLEKVMGPLSTGHLELKTRNP 429

Query: 430 KKSPIVRFNYYSHPDDLAKCVRGVRKVGDLLQTQTMEKIKTQDLEGNKVFQFLGLPWPEN 489
           K +PIV FNY+ HPDDL +CVRG++ +  ++Q++   + K  D+    +   L    P N
Sbjct: 430 KDNPIVTFNYFQHPDDLKRCVRGIQTIERVVQSKAFSRYKYADVSFEYLLN-LTASTPVN 489

Query: 490 L---------SDDSSVEEYCQKTVTTYWHYHGGCLVGKVVDGNFKVIGIENLRVVDGSTF 529
           L         S   S EE+CQ TVTT WHYHGGC+VG+VVDG++KVIGI+ LRV+D ST 
Sbjct: 490 LRPPRSGPGASLPPSAEEFCQHTVTTIWHYHGGCVVGRVVDGDYKVIGIDRLRVIDMSTV 549

BLAST of Tan0003508 vs. TAIR 10
Match: AT1G72970.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 399.4 bits (1025), Expect = 8.6e-111
Identity = 230/526 (43.73%), Postives = 306/526 (58.17%), Query Frame = 0

Query: 46  EKEEYEYIIIGGGTAGCPLAATLSSKFSVLLLERGSDP--NKYPSVLNEQGLLNAFVAED 105
           +   Y+YI+IGGGTAGCPLAATLS  FSVL+LERG  P  N   S L      +  +A+ 
Sbjct: 59  QDSSYDYIVIGGGTAGCPLAATLSQNFSVLVLERGGVPFTNANVSFLRN---FHIGLADI 118

Query: 106 DGENPFQRFISEDGVENIRGRVLGGGSMVNAGFYSRGHREFFETAGVDWDMELVNKSYQW 165
              +  Q F+S DGV N R RVLGGGS +NAGFYSR    F + AG  WD +LV +SY W
Sbjct: 119 SASSASQAFVSTDGVYNARARVLGGGSCINAGFYSRADAAFVKRAG--WDPKLVKESYPW 178

Query: 166 VEQTVVSRPILNAWQSAFRTALLEAGVVPDNEFDLKHLVGTKTGGSIFDDKGNRHGAVEL 225
           VE+ +V +P L  WQ A R +LLE GV P N F   H+ GTK GG+IFD  G RH A EL
Sbjct: 179 VEREIVHQPKLTLWQKALRDSLLEVGVRPFNGFTYDHVSGTKIGGTIFDRFGRRHTAAEL 238

Query: 226 LNKAKPENLKVAIQATVQKIIF--SGL--SASGVSYSDSKGKLHTAFI--RKKGEIILSA 285
           L  A P+ L+V I ATVQKI+F  SG     +GV + D KG  H A +  RK  E+ILS+
Sbjct: 239 LAYANPQKLRVLIYATVQKIVFDTSGTRPRVTGVIFKDEKGNQHQALLSNRKGSEVILSS 298

Query: 286 GAIGSPQLLLLSGIGPKSHLSSLKLPVVLHQPHVGESMSDNPRFGANIMLPFQLVASSVK 345
           GAIGSPQ+L+LSGIGPK  L  LK+PVVL   HVG+ M+DNP     +     +  S ++
Sbjct: 299 GAIGSPQMLMLSGIGPKKELQRLKIPVVLENEHVGKGMADNPMNTILVPSKAPIEQSLIQ 358

Query: 346 VVGTLKNNIHLQAFA--SPSPFLVPPSFSLLPPQST--SIIPSL---------------- 405
            VG  K  ++++A      SP  +   + ++  ++   S IP+                 
Sbjct: 359 TVGITKMGVYVEASTGFGQSPESIHTHYGIMSNKNELFSTIPAKQRRPEATQAYITRNKY 418

Query: 406 --------ALFVGKFSQVLSEGSLRLNSSNVKKSPIVRFNYYSHPDDLAKCVRGVRKVGD 465
                   +  + K +  +S G L L ++NV  +P V FNY+ HP DL +CV  +R V  
Sbjct: 419 QLHEAFNGSFILEKLAYPISRGHLSLVNTNVDDNPSVTFNYFKHPVDLQRCVEAIRLVSK 478

Query: 466 LLQTQTMEKIKTQDLEGNKVFQFLGLP-------WPENLSDDSSVEEYCQKTVTTYWHYH 525
           ++ +     +     +   V + L L         P+ L+D  S+ ++C+ TV T WHYH
Sbjct: 479 VVTSNRF--LNYTQCDKQNVHKMLSLSVKANINLRPKQLNDTKSMAQFCKDTVVTIWHYH 538

Query: 526 GGCLVGKVVDGNFKVIGIENLRVVDGSTFSDSPGTNPMATLMMLGR 529
           GGCLVGKVV  N KV+G++ LRV+DGSTF +SPGTNP AT+MM+GR
Sbjct: 539 GGCLVGKVVSPNRKVLGVDRLRVIDGSTFDESPGTNPQATMMMMGR 577

BLAST of Tan0003508 vs. TAIR 10
Match: AT1G72970.2 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 384.4 bits (986), Expect = 2.9e-106
Identity = 225/519 (43.35%), Postives = 299/519 (57.61%), Query Frame = 0

Query: 46  EKEEYEYIIIGGGTAGCPLAATLSSKFSVLLLERGSDP--NKYPSVLNEQGLLNAFVAED 105
           +   Y+YI+IGGGTAGCPLAATLS  FSVL+LERG  P  N   S L      +  +A+ 
Sbjct: 59  QDSSYDYIVIGGGTAGCPLAATLSQNFSVLVLERGGVPFTNANVSFLRN---FHIGLADI 118

Query: 106 DGENPFQRFISEDGVENIRGRVLGGGSMVNAGFYSRGHREFFETAGVDWDMELVNKSYQW 165
              +  Q F+S DGV N R RVLGGGS +NAGFYSR    F + AG  WD +LV +SY W
Sbjct: 119 SASSASQAFVSTDGVYNARARVLGGGSCINAGFYSRADAAFVKRAG--WDPKLVKESYPW 178

Query: 166 VEQTVVSRPILNAWQSAFRTALLEAGVVPDNEFDLKHLVGTKTGGSIFDDKGNRHGAVEL 225
           VE+ +V +P L  WQ A R +LLE GV P N F   H+ GTK GG+IFD  G RH A EL
Sbjct: 179 VEREIVHQPKLTLWQKALRDSLLEVGVRPFNGFTYDHVSGTKIGGTIFDRFGRRHTAAEL 238

Query: 226 LNKAKPENLKVAIQATVQKIIF--SGL--SASGVSYSDSKGKLHTAFI--RKKGEIILSA 285
           L  A P+ L+V I ATVQKI+F  SG     +GV + D KG  H A +  RK  E+ILS+
Sbjct: 239 LAYANPQKLRVLIYATVQKIVFDTSGTRPRVTGVIFKDEKGNQHQALLSNRKGSEVILSS 298

Query: 286 GAIGSPQLLLLSGIGPKSHLSSLKLPVVLHQPHVGESMSDNPRFGANIMLPFQLVASSVK 345
           GAIGSPQ+L+LSGIGPK  L  LK+PVVL   HVG+ M+DNP     +     +  S ++
Sbjct: 299 GAIGSPQMLMLSGIGPKKELQRLKIPVVLENEHVGKGMADNPMNTILVPSKAPIEQSLIQ 358

Query: 346 VVGTLKNNIHLQAFA--SPSPFLVPPSFSLLPPQST--SIIPSL---------------- 405
            VG  K  ++++A      SP  +   + ++  ++   S IP+                 
Sbjct: 359 TVGITKMGVYVEASTGFGQSPESIHTHYGIMSNKNELFSTIPAKQRRPEATQAYITRNKY 418

Query: 406 --------ALFVGKFSQVLSEGSLRLNSSNVKKSPIVRFNYYSHPDDLAKCVRGVRKVGD 465
                   +  + K +  +S G L L ++NV  +P V FNY+ HP D             
Sbjct: 419 QLHEAFNGSFILEKLAYPISRGHLSLVNTNVDDNPSVTFNYFKHPCD------------- 478

Query: 466 LLQTQTMEKIKTQDLEGNKVFQFLGLPWPENLSDDSSVEEYCQKTVTTYWHYHGGCLVGK 525
               Q + K+ +  ++ N   +      P+ L+D  S+ ++C+ TV T WHYHGGCLVGK
Sbjct: 479 ---KQNVHKMLSLSVKANINLR------PKQLNDTKSMAQFCKDTVVTIWHYHGGCLVGK 538

Query: 526 VVDGNFKVIGIENLRVVDGSTFSDSPGTNPMATLMMLGR 529
           VV  N KV+G++ LRV+DGSTF +SPGTNP AT+MM+GR
Sbjct: 539 VVSPNRKVLGVDRLRVIDGSTFDESPGTNPQATMMMMGR 550

BLAST of Tan0003508 vs. TAIR 10
Match: AT5G51930.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 384.4 bits (986), Expect = 2.9e-106
Identity = 228/555 (41.08%), Postives = 328/555 (59.10%), Query Frame = 0

Query: 9   LLLILISLFQFGVLSSHTISDQDVSYMKLVHNASDIPEKEEYEYIIIGGGTAGCPLAATL 68
           L+L+ IS+F F ++   T      ++MK   +A+  P+   ++YIIIGGGTAGC LAATL
Sbjct: 41  LILVYISIFFFFLVKLET------TFMK---DATLAPKNASFDYIIIGGGTAGCALAATL 100

Query: 69  SSKFSVLLLERGSDPNKYPSVLNEQGLLNAFVAEDDGENPFQRFISEDGVENIRGRVLGG 128
           S   SVL+LERG  P + P+  +    +N  +  +   +  Q FISEDGV N R RVLGG
Sbjct: 101 SQNASVLVLERGGSPYENPTATDMGNSVNTLL-NNTPNSWSQLFISEDGVYNTRPRVLGG 160

Query: 129 GSMVNAGFYSRGHREFFETAGVDWDMELVNKSYQWVEQTVVSRPILNAWQSAFRTALLEA 188
           GS++N GFYSR   ++ E A  +W+ME V  +Y+WVE+ +V  P +  WQ AF+  LLEA
Sbjct: 161 GSVINGGFYSRAGNDYVEEA--EWEMEEVEAAYEWVEKKLVFEPQVIEWQKAFKDGLLEA 220

Query: 189 GVVPDNEFDLKHLVGTKTGGSIFDDKGNRHGAVELLNKAKPENLKVAIQATVQKIIFSGL 248
           G  PDN F   H+ GTK GG+IFD  G+RH A  LL  A P  + V + A+V K++F+  
Sbjct: 221 GESPDNGFTYDHIYGTKIGGTIFDRAGHRHTAANLLEYANPNRIVVYLHASVHKVLFT-T 280

Query: 249 SASGVSYSDSKGKLHTAFIRKK--GEIILSAGAIGSPQLLLLSGIGPKSHLSSLKL-PVV 308
            A  V + D+ G  H A +  K   E+ILSAGA+GSPQLL+LSG+GP  HL +  + P+V
Sbjct: 281 EAYEVLFEDANGVFHKANLANKATNEVILSAGALGSPQLLMLSGVGPAVHLEAHGVNPLV 340

Query: 309 LHQPHVGESMSDNPRFGANIMLPFQLVASSVKVVGTLKNNIHLQAFASPS-PFLVPPSF- 368
           L QP VG+ M+DNP     I  P  +  S ++ VG  K + +++  +  S  F +   F 
Sbjct: 341 LDQPMVGQGMADNPMNFVAIPSPQPVELSLIQAVGITKFDSYIEGLSGLSLSFDITRRFF 400

Query: 369 ----SLLPPQSTS-----IIPSLALFVGKFSQVL-----------------SEGSLRLNS 428
               +LL   S +     +  S+A+ +  F   L                 S+G ++L +
Sbjct: 401 DGVLNLLNETSHTTSRKILTQSIAVLLKSFDVKLEVRMNGGLIFQKVDGPASKGHMKLRN 460

Query: 429 SNVKKSPIVRFNYYSHPDDLAKCVRGVRKVGDLLQTQTMEKIKTQDLEGNKVFQ-FLGLP 488
           +N + +P V FNYY  P+DL KCV+G+  +  ++ ++   K K   +   ++    L LP
Sbjct: 461 TNPRDNPSVTFNYYQEPEDLNKCVKGLNTIIRMINSKAFSKYKYPGVTARELLNLMLALP 520

Query: 489 ---WPENLSDDSSVEEYCQKTVTTYWHYHGGCLVGKVVDGNFKVIGIENLRVVDGSTFSD 529
               P +++   +++++C  TVT+ WHYHGGC VGKVVD N+KV+GI+ LRV+DGSTF  
Sbjct: 521 INLRPRHVTSAFNLKQFCIDTVTSVWHYHGGCQVGKVVDKNYKVLGIDGLRVIDGSTFLK 580