Tan0000306 (gene) Snake gourd v1

BLAST of Tan0000306 vs. ExPASy Swiss-Prot
Match: Q7Y220 (Phospholipase A1 PLIP1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PLIP1 PE=1 SV=1)

HSP 1 Score: 636.0 bits (1639), Expect = 5.0e-181
Identity = 366/691 (52.97%), Postives = 462/691 (66.86%), Query Frame = 0

Query: 1   MACTSVAISNSPAASMAKDIIFKEYNGLRRSHSSNDL-RERACMRRSHSDNHICYSA--- 60
           MA  +   S SPAA  A D++ +E+ GLRRS S  DL  +   +RRS SDNH+C  +   
Sbjct: 1   MAFNTAMASTSPAA--ANDVL-REHIGLRRSLSGQDLVLKGGGIRRSSSDNHLCCRSGNN 60

Query: 61  -NRIHATSTQPKPKNNNNNNSMGMGIFPLKFSGSFLPNSIRSFLFDMEEPSKDLSSEE-- 120
            NRI A S +P  K      S  +G+F  + S S +P+ I++ LF+ +    +  S+E  
Sbjct: 61  NNRILAVSVRPGMK-----TSRSVGVFSFQISSSIIPSPIKTLLFETDTSQDEQESDEIE 120

Query: 121 -------EVTKRANWIERLLQIRSHWRIKQ--QKGGIEIYADHDESGESFCG-GDDGGCE 180
                  +  K+ANW+ERLL+IR  W+ +Q  + G  ++    +ES +  CG  ++ GC 
Sbjct: 121 IETEPNLDGAKKANWVERLLEIRRQWKREQKTESGNSDV---AEESVDVTCGCEEEEGCI 180

Query: 181 VDYYDSEDEGDLSFDTESFARFLVRVPLYDTKVFSQLAFLSNMAYVIPEIKAEDLERYYG 240
            +Y      GD  +  ESF+R LV+V   + K  SQLA+L N+AY IPEIK EDL R YG
Sbjct: 181 ANY--GSVNGD--WGRESFSRLLVKVSWSEAKKLSQLAYLCNLAYTIPEIKGEDLRRNYG 240

Query: 241 LQFVTSSLRKKADAAAINIKEKLDQHSTCISDEAFDVAESCSVEAMELERTQQIPTTVAY 300
           L+FVTSSL KKA AA   ++EKL+Q  T +      V  S  +E+ +  +     +  AY
Sbjct: 241 LKFVTSSLEKKAKAAI--LREKLEQDPTHV-----PVITSPDLESEKQSQRSASSSASAY 300

Query: 301 EIAATAASYAHSRAKNPSSHPSEPQGKGESSTRAYNPEVAAYVAASTMTAVVAAEEVQKQ 360
           +IAA+AASY HS  +   S P             Y    AA  AASTMTAVVAA E +K 
Sbjct: 301 KIAASAASYIHSCKEYDLSEP------------IYKSAAAAQAAASTMTAVVAAGEEEKL 360

Query: 361 ETAKDLQSLHSSPCEWFVCDDTNTLTRCFIIQGSDSLASWQANLFFEPAKFEGTDVLVHR 420
           E A++LQSL SSPCEWFVCDD NT TRCF+IQGSDSLASW+ANLFFEP KFE TDVLVHR
Sbjct: 361 EAARELQSLQSSPCEWFVCDDPNTYTRCFVIQGSDSLASWKANLFFEPTKFEDTDVLVHR 420

Query: 421 GIYEAAKGIYKQFMPEIIDHLKKYANHAKFQFTGHSLGGSLSLLVHLMLLKNGIVKPSML 480
           GIYEAAKGIY+QF+PEI +HL ++ + AKFQFTGHSLGGSLSL+V+LML+  G+V    +
Sbjct: 421 GIYEAAKGIYEQFLPEITEHLSRHGDRAKFQFTGHSLGGSLSLIVNLMLISRGLVSSEAM 480

Query: 481 QPVVTFGSPFVFCGGHKILNELGLVEDDIHCVIMHRDIVPRAFSCNYPNHVAAVLKRLSG 540
           + VVTFGSPFVFCGG KIL ELGL E  +HCV+MHRDIVPRAFSCNYP+HVA VLKRL+G
Sbjct: 481 KSVVTFGSPFVFCGGEKILAELGLDESHVHCVMMHRDIVPRAFSCNYPDHVALVLKRLNG 540

Query: 541 SFRSHSCLNKSKLLYSPLGKLFILQPDERSSPPHPMLPQGSALYTLDSTQYGYSKGLLRA 600
           SFR+H CLNK+KLLYSP+GK++ILQP E  SP HP LP G+ALY L+++  GYS   LRA
Sbjct: 541 SFRTHPCLNKNKLLYSPMGKVYILQPSESVSPTHPWLPPGNALYILENSNEGYSPTALRA 600

Query: 601 FLNCPHPLETLSDPTAYGSEGTILRDHDSSFYLKALNGVLKQHTNMIVGKVKKQRKLLWP 660
           FLN PHPLETLS   AYGSEG++LRDHDS  Y+KA+NGVL+QHT +IV K + QR+ +WP
Sbjct: 601 FLNRPHPLETLSQRAAYGSEGSVLRDHDSKNYVKAVNGVLRQHTKLIVRKARIQRRSVWP 649

Query: 661 LLASPSPELWSHEGNLDNNNNLLSNEIMTGV 675
           +L S    L        N +   + EIMT V
Sbjct: 661 VLTSAGRGL--------NESLTTAEEIMTRV 649

BLAST of Tan0000306 vs. ExPASy Swiss-Prot
Match: F4HXL0 (Phospholipase A1 PLIP2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PLIP2 PE=1 SV=1)

HSP 1 Score: 473.0 bits (1216), Expect = 5.6e-132
Identity = 269/594 (45.29%), Postives = 373/594 (62.79%), Query Frame = 0

Query: 78  GMGIFPLKFSGSFLPNSIRSFLFDMEEPSKDLSSEEEVTKRAN--WIERLLQIRSHWRIK 137
           G GI   + SG  L +++     D  +P  + ++ E  T+R N  W+ ++L ++S W+ +
Sbjct: 62  GGGIASRRRSGLCLDDAVLVDSGDSRKPIAEETAVEMDTERRNGSWVLKILDVQSTWKHE 121

Query: 138 QQKGGIEIY---ADHDESGE---SFCGGDDGGCEVDYY---DSEDEGDLSFDTESFARFL 197
           +++   E+     D DE  E   +    DDGGC+V      D  +      D ESF++ L
Sbjct: 122 EEEDDDEVEDEDGDEDEEVELDDAVVSEDDGGCDVCSVLEDDGNEANKFQLDRESFSKLL 181

Query: 198 VRVPLYDTKVFSQLAFLSNMAYVIPEIKAEDLERYYGLQFVTSSLRKKADAAAINIKEKL 257
            RV L ++K+++QL++L N+AY I +IK  +L +YYGL+FVTSS  K   A        L
Sbjct: 182 RRVTLPESKLYAQLSYLGNLAYSISKIKPANLSKYYGLRFVTSSAEKTESA--------L 241

Query: 258 DQHSTCISDEAFDVAES-CSVEAMELERTQQIPTTVAYEIAATAASYAHSRAKN--PSSH 317
              +  +S E   + E+   VE  E  ++++I  + AYEI A+AASY HSR  N  P + 
Sbjct: 242 KAENGEVSGETKPIVEAEEEVEEEEKNKSRKISASAAYEIVASAASYLHSRTNNILPFNS 301

Query: 318 PSEPQGKGESSTRAYNPEVAAYVAASTMTAVVAAEEVQKQETAKDLQSLHSSPCEWFVCD 377
            S+ +   +      N E ++ VA S +T+VVAAEE  KQ  A DL+S  SSPC+WF+CD
Sbjct: 302 SSKAENSDKHDVNLTNAESSSDVAYS-VTSVVAAEEDVKQAVADDLKSTISSPCDWFICD 361

Query: 378 DTNTLTRCFIIQGSDSLASWQANLFFEPAKFEGTDVLVHRGIYEAAKGIYKQFMPEIIDH 437
           D  + TR  +IQGS+SLASWQANL FEP +FEG   +VHRGIYEAAKG+Y+Q +PE+  H
Sbjct: 362 DDQSHTRFVVIQGSESLASWQANLLFEPIEFEGLGAIVHRGIYEAAKGMYEQMLPEVKAH 421

Query: 438 LKKYANHAKFQFTGHSLGGSLSLLVHLMLLKNGIVKPSMLQPVVTFGSPFVFCGGHKILN 497
           +K +   AKF+FTGHSLGGSLSLL++LMLL  G V  S L PV+T+G+PFV CGG ++L 
Sbjct: 422 IKTHGTSAKFRFTGHSLGGSLSLLLNLMLLVRGEVPASSLLPVITYGAPFVLCGGDRLLK 481

Query: 498 ELGLVEDDIHCVIMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLYSPLGK 557
           +LGL +  +  ++MHRDIVPRAFSCNYP HVA +LK ++G+FRSH CLNK  +LYSP+G+
Sbjct: 482 KLGLPKSHVQAIVMHRDIVPRAFSCNYPYHVAELLKAVNGNFRSHPCLNKQSMLYSPMGE 541

Query: 558 LFILQPDERSSPPHPMLPQGSALYTLDS---------TQYGYSKGLLRAFLNCPHPLETL 617
           L ILQPDE  SP H +LP G+ LY L S         +     +     FLN PHPL+ L
Sbjct: 542 LLILQPDETFSPGHELLPSGNGLYLLTSDFESPDIEDSDEERLRAAQTVFLNTPHPLDIL 601

Query: 618 SDPTAYGSEGTILRDHDSSFYLKALNGVLKQHTNMI-VGKVKKQRKLLWPLLAS 648
           SD +AYGS GTI RDHD + YLKA+  V+++  N I   K + +R L WP+L +
Sbjct: 602 SDRSAYGSSGTIQRDHDMNSYLKAVRSVIRKEVNQIRRAKREHRRSLWWPILVA 646

BLAST of Tan0000306 vs. ExPASy Swiss-Prot
Match: Q940L4 (Phospholipase A1 PLIP3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PLIP3 PE=1 SV=1)

HSP 1 Score: 432.2 bits (1110), Expect = 1.1e-119
Identity = 250/521 (47.98%), Postives = 328/521 (62.96%), Query Frame = 0

Query: 120 NWIERLLQIRSHWRIKQQKGGIEIYADHDE--------SGESFCGGDDGGCEVDYYDSED 179
           NW+ ++L++ S W+ K+Q+ G     + DE          E  C   D  C +D  D ++
Sbjct: 90  NWVLKILEVGSIWKGKRQRSGGGGGGEEDEEEEVAEPKKKEDLCEECD-FCRIDDDDEDE 149

Query: 180 EGDLSFDTESFARFLVRVPLYDTKVFSQLAFLSNMAYVIPEIKAEDLERYYGLQFVTSSL 239
           E + +     F+  L ++P+ D ++F++L+FL N+AY IP+IK E+L +Y  L+FVTSS+
Sbjct: 150 EKEKT--VFEFSEMLSKIPVEDAQMFAKLSFLGNLAYSIPKIKPENLLKYQKLRFVTSSI 209

Query: 240 RKKADAAAINIKEKLDQHSTCISDEAFDVAESCSVEAMELERTQQIPTTVAYEIAATAAS 299
            K+       +  K+++++    DE               E+ + I   VAY IAA+AAS
Sbjct: 210 EKR-------MSLKVEENNNGEEDE---------------EKKKLINPAVAYRIAASAAS 269

Query: 300 --YAHSRAKNPSSHPSEPQGKGESSTRAYNPEVAAYVAASTMTAVVAAEEVQKQETAKDL 359
             ++HS++  P            SS R  N E +    A ++TAVVAA+E  KQ  A DL
Sbjct: 270 RLFSHSKSVLPFG----------SSKRQDNEEASLLATADSVTAVVAAKEEVKQAVADDL 329

Query: 360 QSLHSSPCEWFVCDDTNTLTRCFIIQGSDSLASWQANLFFEPAKFEGTDVLVHRGIYEAA 419
           +S  S PCEWFVCDD  + TR F IQGSDSLASWQANL FEP  FE  DVLVHRGIYEAA
Sbjct: 330 KSNRSPPCEWFVCDDDKSGTRFFFIQGSDSLASWQANLLFEPVPFEDLDVLVHRGIYEAA 389

Query: 420 KGIYKQFMPEIIDHL-KKYANHAKFQFTGHSLGGSLSLLVHLMLLKNGIVKPSMLQPVVT 479
           KGIY+Q +PE+  HL  +  N A  +F+GHSLGGSLSLLV+LMLL  G V  S L PV+T
Sbjct: 390 KGIYEQMLPEVHAHLNSRGKNRAFLRFSGHSLGGSLSLLVNLMLLIRGQVPASSLLPVIT 449

Query: 480 FGSPFVFCGGHKILNELGLVEDDIHCVIMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSH 539
           FGSP + CGG ++L +LGL +  +  + MHRDIVPRAFSCNYPN  A +LK L+G+FR+H
Sbjct: 450 FGSPCIMCGGDRLLQKLGLPKSHLLGISMHRDIVPRAFSCNYPNRAAKLLKALNGNFRNH 509

Query: 540 SCLNKSKLLYSPLGKLFILQPDERSSPPHPMLPQGSALYTLDSTQYGYSKGLLRA----F 599
            CLN   +LYSP+GKL ILQP ER SPPHP+LP GS LY L S     ++  LRA    F
Sbjct: 510 PCLNNQNVLYSPMGKLLILQPSERFSPPHPLLPPGSGLYLLASKNTDETEKSLRAAKILF 569

Query: 600 LNCPHPLETLSDPTAYGSEGTILRDHDSSFYLKALNGVLKQ 626
            N PHPLE LSD  +YGSEG I R+HD S YLKAL  V+++
Sbjct: 570 FNSPHPLEILSDRRSYGSEGKIKRNHDMSSYLKALRHVIRK 575

BLAST of Tan0000306 vs. NCBI nr
Match: XP_004139735.1 (phospholipase A1 PLIP1, chloroplastic [Cucumis sativus] >KGN44827.2 hypothetical protein Csa_015640 [Cucumis sativus])

HSP 1 Score: 1206.0 bits (3119), Expect = 0.0e+00
Identity = 602/676 (89.05%), Postives = 637/676 (94.23%), Query Frame = 0

Query: 1   MACTSVAISNSPAASMAKDIIFKEYNGLRRSHSSNDLRERACMRRSHSDNHICYSANRIH 60
           MACTSVAI NSPAASM K+IIFKEY+ LRRSHSS D+RERAC++RS+SDN ICYSAN+IH
Sbjct: 1   MACTSVAIGNSPAASMTKEIIFKEYSMLRRSHSSKDVRERACIKRSYSDNQICYSANKIH 60

Query: 61  ATSTQPKPKNNNNNNSMGMGIFPLKFSGSFLPNSIRSFLFDMEEPSKDLSSEEEVTKRAN 120
           ATSTQPKPK N NNNS GMGIFPLKFSGSFLPN++RSFLFDMEE SK+L++EEEVTKRAN
Sbjct: 61  ATSTQPKPK-NKNNNSTGMGIFPLKFSGSFLPNAVRSFLFDMEETSKNLTTEEEVTKRAN 120

Query: 121 WIERLLQIRSHWRIKQQKGGIE--IYADHDESGESFCGGDDGGCEVDYYDSEDEGDLSFD 180
           WIERLL+IRS WR+KQQKGG+E  +YADHDES ES CGGDDGGCEVDYYDSEDE  L+FD
Sbjct: 121 WIERLLEIRSRWRLKQQKGGLENDLYADHDESAESLCGGDDGGCEVDYYDSEDEEGLTFD 180

Query: 181 TESFARFLVRVPLYDTKVFSQLAFLSNMAYVIPEIKAEDLERYYGLQFVTSSLRKKADAA 240
           TESFAR+L+RVP+ DTKVFSQLAFLSNMAYVIP IKAEDLE Y+GLQFVTSSL KKA+AA
Sbjct: 181 TESFARYLIRVPVSDTKVFSQLAFLSNMAYVIPNIKAEDLEIYHGLQFVTSSLHKKAEAA 240

Query: 241 AINIKEKLDQHSTCISDEAFDVAESCSVEAMELERTQQIPTTVAYEIAATAASYAHSRAK 300
           AINIKEKLDQHSTCISDEAF+V ESCSVEA++LERTQ+IPTTVAYEIAATAASY HSR K
Sbjct: 241 AINIKEKLDQHSTCISDEAFNVGESCSVEALKLERTQEIPTTVAYEIAATAASYVHSRVK 300

Query: 301 NPSSHPSEPQGKGESSTRAYNPEVAAYVAASTMTAVVAAEEVQKQETAKDLQSLHSSPCE 360
           N SSHP E Q KG+ STR YNPEVAAYVAASTMTAVVAAEEVQKQE AKDLQSLHSSPCE
Sbjct: 301 NTSSHPLESQEKGDGSTRVYNPEVAAYVAASTMTAVVAAEEVQKQEAAKDLQSLHSSPCE 360

Query: 361 WFVCDDTNTLTRCFIIQGSDSLASWQANLFFEPAKFEGTDVLVHRGIYEAAKGIYKQFMP 420
           WFVCDDT+TLTRCFIIQGSDSLASWQANLFFEP KFEGTDVLVHRGIYEAAKGIYKQFMP
Sbjct: 361 WFVCDDTDTLTRCFIIQGSDSLASWQANLFFEPTKFEGTDVLVHRGIYEAAKGIYKQFMP 420

Query: 421 EIIDHLKKYANHAKFQFTGHSLGGSLSLLVHLMLLKNGIVKPSMLQPVVTFGSPFVFCGG 480
           EIIDHLKKY  HA+FQFTGHSLGGSLSLLVHLMLLKNGIVKP+MLQPVVTFGSPFVFCGG
Sbjct: 421 EIIDHLKKYGAHARFQFTGHSLGGSLSLLVHLMLLKNGIVKPTMLQPVVTFGSPFVFCGG 480

Query: 481 HKILNELGLVEDDIHCVIMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLY 540
           HKILNELGL ED+IHC+IMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLY
Sbjct: 481 HKILNELGLDEDNIHCIIMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLY 540

Query: 541 SPLGKLFILQPDERSSPPHPMLPQGSALYTLDSTQYGYSKGLLRAFLNCPHPLETLSDPT 600
           SPLGKLFILQPDE SSPPHPMLPQGSALYTLDSTQ GYSKGLLRAFLNCPHPLETLSDPT
Sbjct: 541 SPLGKLFILQPDEMSSPPHPMLPQGSALYTLDSTQNGYSKGLLRAFLNCPHPLETLSDPT 600

Query: 601 AYGSEGTILRDHDSSFYLKALNGVLKQHTNMIVGKVKKQRKLLWPLLASPSPELWSHEGN 660
           AYGSEGTILRDHDSSFYLKALNGVLKQHT M VGKV+ QRKLLWPLLASPSPELWSH+GN
Sbjct: 601 AYGSEGTILRDHDSSFYLKALNGVLKQHTKMAVGKVRNQRKLLWPLLASPSPELWSHDGN 660

Query: 661 LDNNNNLLSNEIMTGV 675
           LDNNN+LLSNEIMTGV
Sbjct: 661 LDNNNSLLSNEIMTGV 675

BLAST of Tan0000306 vs. NCBI nr
Match: XP_008461523.1 (PREDICTED: uncharacterized protein LOC103500094 [Cucumis melo] >XP_016902691.1 PREDICTED: uncharacterized protein LOC103500094 [Cucumis melo] >XP_016902692.1 PREDICTED: uncharacterized protein LOC103500094 [Cucumis melo])

HSP 1 Score: 1203.0 bits (3111), Expect = 0.0e+00
Identity = 600/676 (88.76%), Postives = 639/676 (94.53%), Query Frame = 0

Query: 1   MACTSVAISNSPAASMAKDIIFKEYNGLRRSHSSNDLRERACMRRSHSDNHICYSANRIH 60
           MACTSVAI NSP A+MAK+IIFKEYNGLRRSHSS D+RERAC++RS+SDN ICYSAN+I+
Sbjct: 1   MACTSVAIGNSPVATMAKEIIFKEYNGLRRSHSSKDVRERACIKRSYSDNQICYSANKIY 60

Query: 61  ATSTQPKPKNNNNNNSMGMGIFPLKFSGSFLPNSIRSFLFDMEEPSKDLSSEEEVTKRAN 120
           ATSTQPKPK   NNNS GMGIFPLKFSGSFLPN++RSFLFDMEE SK+L+SEEEVTKRAN
Sbjct: 61  ATSTQPKPK-IKNNNSTGMGIFPLKFSGSFLPNAVRSFLFDMEETSKNLTSEEEVTKRAN 120

Query: 121 WIERLLQIRSHWRIKQQKGGIE--IYADHDESGESFCGGDDGGCEVDYYDSEDEGDLSFD 180
           WIERLL+IRS WR+KQQKGG+E  +YADHDES ES CGG+DGGCEVDYYDSEDE  L+FD
Sbjct: 121 WIERLLEIRSRWRLKQQKGGLENDLYADHDESAESLCGGEDGGCEVDYYDSEDEEGLTFD 180

Query: 181 TESFARFLVRVPLYDTKVFSQLAFLSNMAYVIPEIKAEDLERYYGLQFVTSSLRKKADAA 240
           TESFAR+L+R+P+ DTKVFSQLAFLSNMAYVIP IKAEDLE Y+GL+FVTSSLRKKA+AA
Sbjct: 181 TESFARYLIRIPVSDTKVFSQLAFLSNMAYVIPNIKAEDLEIYHGLKFVTSSLRKKAEAA 240

Query: 241 AINIKEKLDQHSTCISDEAFDVAESCSVEAMELERTQQIPTTVAYEIAATAASYAHSRAK 300
           AINIKEKLDQHSTCISDEAF+V ESCSVEA E+ERTQ+IPTTVAYEIAATAASY HSRAK
Sbjct: 241 AINIKEKLDQHSTCISDEAFNVGESCSVEASEVERTQEIPTTVAYEIAATAASYVHSRAK 300

Query: 301 NPSSHPSEPQGKGESSTRAYNPEVAAYVAASTMTAVVAAEEVQKQETAKDLQSLHSSPCE 360
           N SSHP E Q KG+ STRAYNPEVAAYVAASTMTAVVAAEEVQKQE AKDLQSLHSSPCE
Sbjct: 301 NTSSHPLESQEKGDGSTRAYNPEVAAYVAASTMTAVVAAEEVQKQEAAKDLQSLHSSPCE 360

Query: 361 WFVCDDTNTLTRCFIIQGSDSLASWQANLFFEPAKFEGTDVLVHRGIYEAAKGIYKQFMP 420
           WFVCDDT+TLTRCFIIQGSDSLASWQANLFFEP KFEGTDVLVHRGIYEAAKGIYKQFMP
Sbjct: 361 WFVCDDTHTLTRCFIIQGSDSLASWQANLFFEPTKFEGTDVLVHRGIYEAAKGIYKQFMP 420

Query: 421 EIIDHLKKYANHAKFQFTGHSLGGSLSLLVHLMLLKNGIVKPSMLQPVVTFGSPFVFCGG 480
           EIIDHLKKY +HA+FQFTGHSLGGSLSLLVHLMLLKNGIVKP+MLQPVVTFGSPFVFCGG
Sbjct: 421 EIIDHLKKYGDHARFQFTGHSLGGSLSLLVHLMLLKNGIVKPTMLQPVVTFGSPFVFCGG 480

Query: 481 HKILNELGLVEDDIHCVIMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLY 540
           HKILNELGL ED+IHC+IMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLY
Sbjct: 481 HKILNELGLDEDNIHCIIMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLY 540

Query: 541 SPLGKLFILQPDERSSPPHPMLPQGSALYTLDSTQYGYSKGLLRAFLNCPHPLETLSDPT 600
           SPLGKLFILQPDE SSPPHPMLPQGSALYTLDSTQ GYSKGLLRAFLNCPHPLETLSDPT
Sbjct: 541 SPLGKLFILQPDEMSSPPHPMLPQGSALYTLDSTQNGYSKGLLRAFLNCPHPLETLSDPT 600

Query: 601 AYGSEGTILRDHDSSFYLKALNGVLKQHTNMIVGKVKKQRKLLWPLLASPSPELWSHEGN 660
           AYGSEGTILRDHDSSFYLKALNGVLKQHT M VGKV+ QRKLLWPLLASPSP+ WSH+GN
Sbjct: 601 AYGSEGTILRDHDSSFYLKALNGVLKQHTKMGVGKVRNQRKLLWPLLASPSPDRWSHDGN 660

Query: 661 LDNNNNLLSNEIMTGV 675
           LDNNN+LLSNEIMTGV
Sbjct: 661 LDNNNSLLSNEIMTGV 675

BLAST of Tan0000306 vs. NCBI nr
Match: KAA0051002.1 (uncharacterized protein E6C27_scaffold2606G00220 [Cucumis melo var. makuwa] >TYK04390.1 uncharacterized protein E5676_scaffold675G00720 [Cucumis melo var. makuwa])

HSP 1 Score: 1196.4 bits (3094), Expect = 0.0e+00
Identity = 599/676 (88.61%), Postives = 639/676 (94.53%), Query Frame = 0

Query: 1   MACTSVAISNSPAASMAKDIIFKEYNGLRRSHSSNDLRERACMRRSHSDNHICYSANRIH 60
           MACTSVAI NSP A+MAK+IIFKEYNGLRRSHSS D+RERAC++RS+SDN ICYSAN+I+
Sbjct: 1   MACTSVAIGNSPVATMAKEIIFKEYNGLRRSHSSKDVRERACIKRSYSDNQICYSANKIY 60

Query: 61  ATSTQPKPKNNNNNNSMGMGIFPLKFSGSFLPNSIRSFLFDMEEPSKDLSSEEEVTKRAN 120
           ATSTQPKPK   NNNS GMGIFPLKFSGSFLPN++RSFLFDMEE SK+L+SEEEVTKRAN
Sbjct: 61  ATSTQPKPK-IKNNNSTGMGIFPLKFSGSFLPNAVRSFLFDMEETSKNLTSEEEVTKRAN 120

Query: 121 WIERLLQIRSHWRIKQQKGGIE--IYADHDESGESFCGGDDGGCEVDYYDSEDEGDLSFD 180
           WIERLL+IRS WR+KQQKGG+E  +YAD DES ES CGG+DGGCEVDYYDSEDE  L+FD
Sbjct: 121 WIERLLEIRSRWRLKQQKGGLENDLYAD-DESAESLCGGEDGGCEVDYYDSEDEEGLTFD 180

Query: 181 TESFARFLVRVPLYDTKVFSQLAFLSNMAYVIPEIKAEDLERYYGLQFVTSSLRKKADAA 240
           TESFAR+L+R+P+ DTKVFSQLAFLSNMAYVIP IKAEDLE Y+GL+FVTSSLRKKA+AA
Sbjct: 181 TESFARYLIRIPVSDTKVFSQLAFLSNMAYVIPNIKAEDLEIYHGLKFVTSSLRKKAEAA 240

Query: 241 AINIKEKLDQHSTCISDEAFDVAESCSVEAMELERTQQIPTTVAYEIAATAASYAHSRAK 300
           AINIKEKLDQHSTCISDEAF+V ESCSVEA+E+ERTQ+IPTTVAYEIAATAASY HSRAK
Sbjct: 241 AINIKEKLDQHSTCISDEAFNVGESCSVEALEVERTQEIPTTVAYEIAATAASYVHSRAK 300

Query: 301 NPSSHPSEPQGKGESSTRAYNPEVAAYVAASTMTAVVAAEEVQKQETAKDLQSLHSSPCE 360
           N SSHP E Q KG+ STRAYNPEVAAYVAASTMTAVVAAEEVQKQE AKDLQSLHSSPCE
Sbjct: 301 NTSSHPLESQEKGDGSTRAYNPEVAAYVAASTMTAVVAAEEVQKQEAAKDLQSLHSSPCE 360

Query: 361 WFVCDDTNTLTRCFIIQGSDSLASWQANLFFEPAKFEGTDVLVHRGIYEAAKGIYKQFMP 420
           WFVCDDT+TLTRCFIIQGSDSLASWQANLFFEP KFEGTDVLVHRGIYEAAKGIYKQFMP
Sbjct: 361 WFVCDDTHTLTRCFIIQGSDSLASWQANLFFEPTKFEGTDVLVHRGIYEAAKGIYKQFMP 420

Query: 421 EIIDHLKKYANHAKFQFTGHSLGGSLSLLVHLMLLKNGIVKPSMLQPVVTFGSPFVFCGG 480
           EIIDHLKKY +HA+FQFTGHSLGGSLSLLVHLMLLKNGIVKP+MLQPVVTFGSPFVFCGG
Sbjct: 421 EIIDHLKKYGDHARFQFTGHSLGGSLSLLVHLMLLKNGIVKPTMLQPVVTFGSPFVFCGG 480

Query: 481 HKILNELGLVEDDIHCVIMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLY 540
           HKILNELGL ED+IHC+IMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLY
Sbjct: 481 HKILNELGLDEDNIHCIIMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLY 540

Query: 541 SPLGKLFILQPDERSSPPHPMLPQGSALYTLDSTQYGYSKGLLRAFLNCPHPLETLSDPT 600
           SPLGKLFILQPDE SSPPHPMLPQGSALYTLDSTQ GYSKGLLRAFLNCPHPLETLSDPT
Sbjct: 541 SPLGKLFILQPDEMSSPPHPMLPQGSALYTLDSTQNGYSKGLLRAFLNCPHPLETLSDPT 600

Query: 601 AYGSEGTILRDHDSSFYLKALNGVLKQHTNMIVGKVKKQRKLLWPLLASPSPELWSHEGN 660
           AYGSEGTILRDHDSSFYLKALNGVLKQHT M VGKV+ QRKLLWPLLASPSP+ WSH+GN
Sbjct: 601 AYGSEGTILRDHDSSFYLKALNGVLKQHTKMGVGKVRNQRKLLWPLLASPSPDRWSHDGN 660

Query: 661 LDNNNNLLSNEIMTGV 675
           LDNNN+LLSNEIMTGV
Sbjct: 661 LDNNNSLLSNEIMTGV 674

BLAST of Tan0000306 vs. NCBI nr
Match: XP_038897374.1 (phospholipase A1 PLIP1, chloroplastic [Benincasa hispida])

HSP 1 Score: 1192.2 bits (3083), Expect = 0.0e+00
Identity = 603/677 (89.07%), Postives = 634/677 (93.65%), Query Frame = 0

Query: 1   MACTSVAISNSPAASMAKDIIFKEYNGLRRSHSSNDLRERACMRRSHSDNHICYSANRIH 60
           MACTSVAI NSPAA++ KDIIFKEYNGLRRSHSS D+RERAC++RS+SDN ICYSAN+IH
Sbjct: 1   MACTSVAIGNSPAATITKDIIFKEYNGLRRSHSSKDIRERACIKRSYSDNQICYSANKIH 60

Query: 61  ATSTQPKPKNNNNNNSMGMGIFPLKFSGSFLPNSIRSFLFDMEEPSKDLSSEEEVTKRAN 120
           A STQPKPK N+NNNSMG GIFPLKFSGSFLPN++RSFLFDMEE SKDL+ EEEVTKRAN
Sbjct: 61  ANSTQPKPK-NSNNNSMGTGIFPLKFSGSFLPNAVRSFLFDMEETSKDLTGEEEVTKRAN 120

Query: 121 WIERLLQIRSHWRIK-QQKGGIE--IYADHDESGESFCGGDDGGCEVDYYDSEDEGDLSF 180
           WIERLL+IRS WRIK QQKGG+E  +YADHDE  ES  GGDD  CEVDYYDSEDE  L+F
Sbjct: 121 WIERLLEIRSRWRIKQQQKGGVENDLYADHDEYTESVFGGDDDVCEVDYYDSEDEEGLTF 180

Query: 181 DTESFARFLVRVPLYDTKVFSQLAFLSNMAYVIPEIKAEDLERYYGLQFVTSSLRKKADA 240
           DTE FARFL+RVPL DTKVFS+LAFLSNMAYVIP+IKAEDLE Y+GLQFVTSSLRKKADA
Sbjct: 181 DTELFARFLIRVPLSDTKVFSRLAFLSNMAYVIPKIKAEDLEIYHGLQFVTSSLRKKADA 240

Query: 241 AAINIKEKLDQHSTCISDEAFDVAESCSVEAMELERTQQIPTTVAYEIAATAASYAHSRA 300
           AAINIKEKLDQ STCISDEAF+V ES SVEA+EL RT+QIPTTVAYEIAATAASY HSRA
Sbjct: 241 AAINIKEKLDQQSTCISDEAFNVDESFSVEALELGRTRQIPTTVAYEIAATAASYVHSRA 300

Query: 301 KNPSSHPSEPQGKGESSTRAYNPEVAAYVAASTMTAVVAAEEVQKQETAKDLQSLHSSPC 360
           KNPSSHP E Q KGESSTRAYNPEVAAYVAASTMTAVVAAEEVQKQETAKDLQSLHSSPC
Sbjct: 301 KNPSSHPLESQEKGESSTRAYNPEVAAYVAASTMTAVVAAEEVQKQETAKDLQSLHSSPC 360

Query: 361 EWFVCDDTNTLTRCFIIQGSDSLASWQANLFFEPAKFEGTDVLVHRGIYEAAKGIYKQFM 420
           EWF+CDDT+TLTRCFIIQGSDSLASWQANLFFEP KFEGT+VLVHRGIYEAAKGIYKQFM
Sbjct: 361 EWFICDDTHTLTRCFIIQGSDSLASWQANLFFEPTKFEGTEVLVHRGIYEAAKGIYKQFM 420

Query: 421 PEIIDHLKKYANHAKFQFTGHSLGGSLSLLVHLMLLKNGIVKPSMLQPVVTFGSPFVFCG 480
           PEIIDHLKKY  HAKFQFTGHSLGGSLSLLVHLMLLKNG+VKP+MLQPVVTFGSPFVFCG
Sbjct: 421 PEIIDHLKKYGTHAKFQFTGHSLGGSLSLLVHLMLLKNGVVKPTMLQPVVTFGSPFVFCG 480

Query: 481 GHKILNELGLVEDDIHCVIMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLL 540
           GHKILNELGL E+DIHCVIMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLL
Sbjct: 481 GHKILNELGLDENDIHCVIMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLL 540

Query: 541 YSPLGKLFILQPDERSSPPHPMLPQGSALYTLDSTQYGYSKGLLRAFLNCPHPLETLSDP 600
           YSPLGKLFILQPDE SSPPHPMLPQGSALYTLDSTQ GYSKGLLRAFLNCPHPLETLSDP
Sbjct: 541 YSPLGKLFILQPDEMSSPPHPMLPQGSALYTLDSTQNGYSKGLLRAFLNCPHPLETLSDP 600

Query: 601 TAYGSEGTILRDHDSSFYLKALNGVLKQHTNMIVGKVKKQRKLLWPLLASPSPELWSHEG 660
           TAYGSEGTILRDHDSSFYLKALNGVLKQ T M VGKV+ QRKLLWPLLASPSPELWSH+G
Sbjct: 601 TAYGSEGTILRDHDSSFYLKALNGVLKQQTKMTVGKVRNQRKLLWPLLASPSPELWSHDG 660

Query: 661 NLDNNNNLLSNEIMTGV 675
           NLDNNN+ LSNEIMTGV
Sbjct: 661 NLDNNNSFLSNEIMTGV 676

BLAST of Tan0000306 vs. NCBI nr
Match: XP_022992170.1 (uncharacterized protein LOC111488580 isoform X1 [Cucurbita maxima])

HSP 1 Score: 1155.2 bits (2987), Expect = 0.0e+00
Identity = 582/676 (86.09%), Postives = 618/676 (91.42%), Query Frame = 0

Query: 1   MACTSVAISNSPAASMAKDIIFKEYNGLRRSHSSNDLRERACMRRSHSDNHICYSANRIH 60
           MACTSVAI NS A SM KDIIFKEYNG+RRSHSS D+RER C++RS+SDNHICYSAN+IH
Sbjct: 34  MACTSVAIGNSSATSMTKDIIFKEYNGIRRSHSSKDIRERTCIKRSYSDNHICYSANKIH 93

Query: 61  ATSTQPKPK-NNNNNNSMGMGIFPLKFSGSFLPNSIRSFLFDMEEPSKDLSSEEEVTKRA 120
           ATSTQPKPK NNNNNNSMGM IFPLKFSGSFLP+ IRSFLFDMEE SKDLSSEEEVT+RA
Sbjct: 94  ATSTQPKPKNNNNNNNSMGMRIFPLKFSGSFLPHPIRSFLFDMEETSKDLSSEEEVTRRA 153

Query: 121 NWIERLLQIRSHWRIKQQKG-GIEIYADHDESGESFCGGDDGGCEVDYYDSEDEGDLSFD 180
           NWIERLL+IR HWR KQ+KG   +IY  HDE+GE FCG DDGGCEVDYYDSEDEG+ SFD
Sbjct: 154 NWIERLLEIRRHWRTKQKKGVENDIYTHHDETGERFCGEDDGGCEVDYYDSEDEGEFSFD 213

Query: 181 TESFARFLVRVPLYDTKVFSQLAFLSNMAYVIPEIKAEDLERYYGLQFVTSSLRKKADAA 240
           TESFARFLV+VPL DTKVFSQLAFLSNMAYVIPEIKAE+LER  GL+FVTSSLRKKA+ A
Sbjct: 214 TESFARFLVKVPLSDTKVFSQLAFLSNMAYVIPEIKAEELERCCGLKFVTSSLRKKANGA 273

Query: 241 AINIKEKLDQHSTCISDEAFDVAESCSVEAMELERTQQIPTTVAYEIAATAASYAHSRAK 300
           A N+KEK DQHSTCISDEA +V E CS EA++LERTQQIPTTVAYEIAATAASY HSRAK
Sbjct: 274 AFNMKEKQDQHSTCISDEALNVGEVCSGEALQLERTQQIPTTVAYEIAATAASYMHSRAK 333

Query: 301 NPSSHPSEPQGKGESSTRAYNPEVAAYVAASTMTAVVAAEEVQKQETAKDLQSLHSSPCE 360
           NPSSHP EPQ +GESS RAYNPEVAAYVAASTMTAVVAAEEVQKQETAK LQSLHSSPCE
Sbjct: 334 NPSSHPLEPQERGESSCRAYNPEVAAYVAASTMTAVVAAEEVQKQETAKGLQSLHSSPCE 393

Query: 361 WFVCDDTNTLTRCFIIQGSDSLASWQANLFFEPAKFEGTDVLVHRGIYEAAKGIYKQFMP 420
           WFVCDD ++ TR FIIQGSDSLASWQANLFFEP KFEG+DVLVHRGIYEAAKGIYKQF+P
Sbjct: 394 WFVCDDIHSSTRYFIIQGSDSLASWQANLFFEPTKFEGSDVLVHRGIYEAAKGIYKQFIP 453

Query: 421 EIIDHLKKYANHAKFQFTGHSLGGSLSLLVHLMLLKNGIVKPSMLQPVVTFGSPFVFCGG 480
           EII+HLK Y  HAKFQFTGHSLGGSLSLLVHLMLLKN IVKP MLQPVVTFGSPFVFCGG
Sbjct: 454 EIIEHLKTYGTHAKFQFTGHSLGGSLSLLVHLMLLKNEIVKPCMLQPVVTFGSPFVFCGG 513

Query: 481 HKILNELGLVEDDIHCVIMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLY 540
           HKIL ELGL EDDI CVIMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLY
Sbjct: 514 HKILIELGLDEDDIRCVIMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLY 573

Query: 541 SPLGKLFILQPDERSSPPHPMLPQGSALYTLDSTQYGYSKGLLRAFLNCPHPLETLSDPT 600
           SP+GK+FILQP+E SSPPHPMLP+GSALYTLDSTQ GYS G+LRAFLNCPHPLETLSDPT
Sbjct: 574 SPMGKIFILQPEEMSSPPHPMLPEGSALYTLDSTQNGYSTGILRAFLNCPHPLETLSDPT 633

Query: 601 AYGSEGTILRDHDSSFYLKALNGVLKQHTNMIVGKVKKQRKLLWPLLASPSPELWSHEGN 660
           AYGSEGTILRDHDSSFYLKALNGVL QHT M  GKV+KQRKLL PLL SPSPELW H+GN
Sbjct: 634 AYGSEGTILRDHDSSFYLKALNGVLNQHTKMHTGKVRKQRKLLLPLLTSPSPELWRHDGN 693

Query: 661 LDNNNNLLSNEIMTGV 675
           LDN N+L+SNEIMTGV
Sbjct: 694 LDNKNSLVSNEIMTGV 709

BLAST of Tan0000306 vs. ExPASy TrEMBL
Match: A0A0A0K4E8 (Lipase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G325710 PE=4 SV=1)

HSP 1 Score: 1206.0 bits (3119), Expect = 0.0e+00
Identity = 602/676 (89.05%), Postives = 637/676 (94.23%), Query Frame = 0

Query: 1   MACTSVAISNSPAASMAKDIIFKEYNGLRRSHSSNDLRERACMRRSHSDNHICYSANRIH 60
           MACTSVAI NSPAASM K+IIFKEY+ LRRSHSS D+RERAC++RS+SDN ICYSAN+IH
Sbjct: 38  MACTSVAIGNSPAASMTKEIIFKEYSMLRRSHSSKDVRERACIKRSYSDNQICYSANKIH 97

Query: 61  ATSTQPKPKNNNNNNSMGMGIFPLKFSGSFLPNSIRSFLFDMEEPSKDLSSEEEVTKRAN 120
           ATSTQPKPK N NNNS GMGIFPLKFSGSFLPN++RSFLFDMEE SK+L++EEEVTKRAN
Sbjct: 98  ATSTQPKPK-NKNNNSTGMGIFPLKFSGSFLPNAVRSFLFDMEETSKNLTTEEEVTKRAN 157

Query: 121 WIERLLQIRSHWRIKQQKGGIE--IYADHDESGESFCGGDDGGCEVDYYDSEDEGDLSFD 180
           WIERLL+IRS WR+KQQKGG+E  +YADHDES ES CGGDDGGCEVDYYDSEDE  L+FD
Sbjct: 158 WIERLLEIRSRWRLKQQKGGLENDLYADHDESAESLCGGDDGGCEVDYYDSEDEEGLTFD 217

Query: 181 TESFARFLVRVPLYDTKVFSQLAFLSNMAYVIPEIKAEDLERYYGLQFVTSSLRKKADAA 240
           TESFAR+L+RVP+ DTKVFSQLAFLSNMAYVIP IKAEDLE Y+GLQFVTSSL KKA+AA
Sbjct: 218 TESFARYLIRVPVSDTKVFSQLAFLSNMAYVIPNIKAEDLEIYHGLQFVTSSLHKKAEAA 277

Query: 241 AINIKEKLDQHSTCISDEAFDVAESCSVEAMELERTQQIPTTVAYEIAATAASYAHSRAK 300
           AINIKEKLDQHSTCISDEAF+V ESCSVEA++LERTQ+IPTTVAYEIAATAASY HSR K
Sbjct: 278 AINIKEKLDQHSTCISDEAFNVGESCSVEALKLERTQEIPTTVAYEIAATAASYVHSRVK 337

Query: 301 NPSSHPSEPQGKGESSTRAYNPEVAAYVAASTMTAVVAAEEVQKQETAKDLQSLHSSPCE 360
           N SSHP E Q KG+ STR YNPEVAAYVAASTMTAVVAAEEVQKQE AKDLQSLHSSPCE
Sbjct: 338 NTSSHPLESQEKGDGSTRVYNPEVAAYVAASTMTAVVAAEEVQKQEAAKDLQSLHSSPCE 397

Query: 361 WFVCDDTNTLTRCFIIQGSDSLASWQANLFFEPAKFEGTDVLVHRGIYEAAKGIYKQFMP 420
           WFVCDDT+TLTRCFIIQGSDSLASWQANLFFEP KFEGTDVLVHRGIYEAAKGIYKQFMP
Sbjct: 398 WFVCDDTDTLTRCFIIQGSDSLASWQANLFFEPTKFEGTDVLVHRGIYEAAKGIYKQFMP 457

Query: 421 EIIDHLKKYANHAKFQFTGHSLGGSLSLLVHLMLLKNGIVKPSMLQPVVTFGSPFVFCGG 480
           EIIDHLKKY  HA+FQFTGHSLGGSLSLLVHLMLLKNGIVKP+MLQPVVTFGSPFVFCGG
Sbjct: 458 EIIDHLKKYGAHARFQFTGHSLGGSLSLLVHLMLLKNGIVKPTMLQPVVTFGSPFVFCGG 517

Query: 481 HKILNELGLVEDDIHCVIMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLY 540
           HKILNELGL ED+IHC+IMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLY
Sbjct: 518 HKILNELGLDEDNIHCIIMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLY 577

Query: 541 SPLGKLFILQPDERSSPPHPMLPQGSALYTLDSTQYGYSKGLLRAFLNCPHPLETLSDPT 600
           SPLGKLFILQPDE SSPPHPMLPQGSALYTLDSTQ GYSKGLLRAFLNCPHPLETLSDPT
Sbjct: 578 SPLGKLFILQPDEMSSPPHPMLPQGSALYTLDSTQNGYSKGLLRAFLNCPHPLETLSDPT 637

Query: 601 AYGSEGTILRDHDSSFYLKALNGVLKQHTNMIVGKVKKQRKLLWPLLASPSPELWSHEGN 660
           AYGSEGTILRDHDSSFYLKALNGVLKQHT M VGKV+ QRKLLWPLLASPSPELWSH+GN
Sbjct: 638 AYGSEGTILRDHDSSFYLKALNGVLKQHTKMAVGKVRNQRKLLWPLLASPSPELWSHDGN 697

Query: 661 LDNNNNLLSNEIMTGV 675
           LDNNN+LLSNEIMTGV
Sbjct: 698 LDNNNSLLSNEIMTGV 712

BLAST of Tan0000306 vs. ExPASy TrEMBL
Match: A0A1S4E386 (uncharacterized protein LOC103500094 OS=Cucumis melo OX=3656 GN=LOC103500094 PE=4 SV=1)

HSP 1 Score: 1203.0 bits (3111), Expect = 0.0e+00
Identity = 600/676 (88.76%), Postives = 639/676 (94.53%), Query Frame = 0

Query: 1   MACTSVAISNSPAASMAKDIIFKEYNGLRRSHSSNDLRERACMRRSHSDNHICYSANRIH 60
           MACTSVAI NSP A+MAK+IIFKEYNGLRRSHSS D+RERAC++RS+SDN ICYSAN+I+
Sbjct: 1   MACTSVAIGNSPVATMAKEIIFKEYNGLRRSHSSKDVRERACIKRSYSDNQICYSANKIY 60

Query: 61  ATSTQPKPKNNNNNNSMGMGIFPLKFSGSFLPNSIRSFLFDMEEPSKDLSSEEEVTKRAN 120
           ATSTQPKPK   NNNS GMGIFPLKFSGSFLPN++RSFLFDMEE SK+L+SEEEVTKRAN
Sbjct: 61  ATSTQPKPK-IKNNNSTGMGIFPLKFSGSFLPNAVRSFLFDMEETSKNLTSEEEVTKRAN 120

Query: 121 WIERLLQIRSHWRIKQQKGGIE--IYADHDESGESFCGGDDGGCEVDYYDSEDEGDLSFD 180
           WIERLL+IRS WR+KQQKGG+E  +YADHDES ES CGG+DGGCEVDYYDSEDE  L+FD
Sbjct: 121 WIERLLEIRSRWRLKQQKGGLENDLYADHDESAESLCGGEDGGCEVDYYDSEDEEGLTFD 180

Query: 181 TESFARFLVRVPLYDTKVFSQLAFLSNMAYVIPEIKAEDLERYYGLQFVTSSLRKKADAA 240
           TESFAR+L+R+P+ DTKVFSQLAFLSNMAYVIP IKAEDLE Y+GL+FVTSSLRKKA+AA
Sbjct: 181 TESFARYLIRIPVSDTKVFSQLAFLSNMAYVIPNIKAEDLEIYHGLKFVTSSLRKKAEAA 240

Query: 241 AINIKEKLDQHSTCISDEAFDVAESCSVEAMELERTQQIPTTVAYEIAATAASYAHSRAK 300
           AINIKEKLDQHSTCISDEAF+V ESCSVEA E+ERTQ+IPTTVAYEIAATAASY HSRAK
Sbjct: 241 AINIKEKLDQHSTCISDEAFNVGESCSVEASEVERTQEIPTTVAYEIAATAASYVHSRAK 300

Query: 301 NPSSHPSEPQGKGESSTRAYNPEVAAYVAASTMTAVVAAEEVQKQETAKDLQSLHSSPCE 360
           N SSHP E Q KG+ STRAYNPEVAAYVAASTMTAVVAAEEVQKQE AKDLQSLHSSPCE
Sbjct: 301 NTSSHPLESQEKGDGSTRAYNPEVAAYVAASTMTAVVAAEEVQKQEAAKDLQSLHSSPCE 360

Query: 361 WFVCDDTNTLTRCFIIQGSDSLASWQANLFFEPAKFEGTDVLVHRGIYEAAKGIYKQFMP 420
           WFVCDDT+TLTRCFIIQGSDSLASWQANLFFEP KFEGTDVLVHRGIYEAAKGIYKQFMP
Sbjct: 361 WFVCDDTHTLTRCFIIQGSDSLASWQANLFFEPTKFEGTDVLVHRGIYEAAKGIYKQFMP 420

Query: 421 EIIDHLKKYANHAKFQFTGHSLGGSLSLLVHLMLLKNGIVKPSMLQPVVTFGSPFVFCGG 480
           EIIDHLKKY +HA+FQFTGHSLGGSLSLLVHLMLLKNGIVKP+MLQPVVTFGSPFVFCGG
Sbjct: 421 EIIDHLKKYGDHARFQFTGHSLGGSLSLLVHLMLLKNGIVKPTMLQPVVTFGSPFVFCGG 480

Query: 481 HKILNELGLVEDDIHCVIMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLY 540
           HKILNELGL ED+IHC+IMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLY
Sbjct: 481 HKILNELGLDEDNIHCIIMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLY 540

Query: 541 SPLGKLFILQPDERSSPPHPMLPQGSALYTLDSTQYGYSKGLLRAFLNCPHPLETLSDPT 600
           SPLGKLFILQPDE SSPPHPMLPQGSALYTLDSTQ GYSKGLLRAFLNCPHPLETLSDPT
Sbjct: 541 SPLGKLFILQPDEMSSPPHPMLPQGSALYTLDSTQNGYSKGLLRAFLNCPHPLETLSDPT 600

Query: 601 AYGSEGTILRDHDSSFYLKALNGVLKQHTNMIVGKVKKQRKLLWPLLASPSPELWSHEGN 660
           AYGSEGTILRDHDSSFYLKALNGVLKQHT M VGKV+ QRKLLWPLLASPSP+ WSH+GN
Sbjct: 601 AYGSEGTILRDHDSSFYLKALNGVLKQHTKMGVGKVRNQRKLLWPLLASPSPDRWSHDGN 660

Query: 661 LDNNNNLLSNEIMTGV 675
           LDNNN+LLSNEIMTGV
Sbjct: 661 LDNNNSLLSNEIMTGV 675

BLAST of Tan0000306 vs. ExPASy TrEMBL
Match: A0A5D3BZ41 (Lipase_3 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold675G00720 PE=4 SV=1)

HSP 1 Score: 1196.4 bits (3094), Expect = 0.0e+00
Identity = 599/676 (88.61%), Postives = 639/676 (94.53%), Query Frame = 0

Query: 1   MACTSVAISNSPAASMAKDIIFKEYNGLRRSHSSNDLRERACMRRSHSDNHICYSANRIH 60
           MACTSVAI NSP A+MAK+IIFKEYNGLRRSHSS D+RERAC++RS+SDN ICYSAN+I+
Sbjct: 1   MACTSVAIGNSPVATMAKEIIFKEYNGLRRSHSSKDVRERACIKRSYSDNQICYSANKIY 60

Query: 61  ATSTQPKPKNNNNNNSMGMGIFPLKFSGSFLPNSIRSFLFDMEEPSKDLSSEEEVTKRAN 120
           ATSTQPKPK   NNNS GMGIFPLKFSGSFLPN++RSFLFDMEE SK+L+SEEEVTKRAN
Sbjct: 61  ATSTQPKPK-IKNNNSTGMGIFPLKFSGSFLPNAVRSFLFDMEETSKNLTSEEEVTKRAN 120

Query: 121 WIERLLQIRSHWRIKQQKGGIE--IYADHDESGESFCGGDDGGCEVDYYDSEDEGDLSFD 180
           WIERLL+IRS WR+KQQKGG+E  +YAD DES ES CGG+DGGCEVDYYDSEDE  L+FD
Sbjct: 121 WIERLLEIRSRWRLKQQKGGLENDLYAD-DESAESLCGGEDGGCEVDYYDSEDEEGLTFD 180

Query: 181 TESFARFLVRVPLYDTKVFSQLAFLSNMAYVIPEIKAEDLERYYGLQFVTSSLRKKADAA 240
           TESFAR+L+R+P+ DTKVFSQLAFLSNMAYVIP IKAEDLE Y+GL+FVTSSLRKKA+AA
Sbjct: 181 TESFARYLIRIPVSDTKVFSQLAFLSNMAYVIPNIKAEDLEIYHGLKFVTSSLRKKAEAA 240

Query: 241 AINIKEKLDQHSTCISDEAFDVAESCSVEAMELERTQQIPTTVAYEIAATAASYAHSRAK 300
           AINIKEKLDQHSTCISDEAF+V ESCSVEA+E+ERTQ+IPTTVAYEIAATAASY HSRAK
Sbjct: 241 AINIKEKLDQHSTCISDEAFNVGESCSVEALEVERTQEIPTTVAYEIAATAASYVHSRAK 300

Query: 301 NPSSHPSEPQGKGESSTRAYNPEVAAYVAASTMTAVVAAEEVQKQETAKDLQSLHSSPCE 360
           N SSHP E Q KG+ STRAYNPEVAAYVAASTMTAVVAAEEVQKQE AKDLQSLHSSPCE
Sbjct: 301 NTSSHPLESQEKGDGSTRAYNPEVAAYVAASTMTAVVAAEEVQKQEAAKDLQSLHSSPCE 360

Query: 361 WFVCDDTNTLTRCFIIQGSDSLASWQANLFFEPAKFEGTDVLVHRGIYEAAKGIYKQFMP 420
           WFVCDDT+TLTRCFIIQGSDSLASWQANLFFEP KFEGTDVLVHRGIYEAAKGIYKQFMP
Sbjct: 361 WFVCDDTHTLTRCFIIQGSDSLASWQANLFFEPTKFEGTDVLVHRGIYEAAKGIYKQFMP 420

Query: 421 EIIDHLKKYANHAKFQFTGHSLGGSLSLLVHLMLLKNGIVKPSMLQPVVTFGSPFVFCGG 480
           EIIDHLKKY +HA+FQFTGHSLGGSLSLLVHLMLLKNGIVKP+MLQPVVTFGSPFVFCGG
Sbjct: 421 EIIDHLKKYGDHARFQFTGHSLGGSLSLLVHLMLLKNGIVKPTMLQPVVTFGSPFVFCGG 480

Query: 481 HKILNELGLVEDDIHCVIMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLY 540
           HKILNELGL ED+IHC+IMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLY
Sbjct: 481 HKILNELGLDEDNIHCIIMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLY 540

Query: 541 SPLGKLFILQPDERSSPPHPMLPQGSALYTLDSTQYGYSKGLLRAFLNCPHPLETLSDPT 600
           SPLGKLFILQPDE SSPPHPMLPQGSALYTLDSTQ GYSKGLLRAFLNCPHPLETLSDPT
Sbjct: 541 SPLGKLFILQPDEMSSPPHPMLPQGSALYTLDSTQNGYSKGLLRAFLNCPHPLETLSDPT 600

Query: 601 AYGSEGTILRDHDSSFYLKALNGVLKQHTNMIVGKVKKQRKLLWPLLASPSPELWSHEGN 660
           AYGSEGTILRDHDSSFYLKALNGVLKQHT M VGKV+ QRKLLWPLLASPSP+ WSH+GN
Sbjct: 601 AYGSEGTILRDHDSSFYLKALNGVLKQHTKMGVGKVRNQRKLLWPLLASPSPDRWSHDGN 660

Query: 661 LDNNNNLLSNEIMTGV 675
           LDNNN+LLSNEIMTGV
Sbjct: 661 LDNNNSLLSNEIMTGV 674

BLAST of Tan0000306 vs. ExPASy TrEMBL
Match: A0A6J1JSU1 (uncharacterized protein LOC111488580 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111488580 PE=4 SV=1)

HSP 1 Score: 1155.2 bits (2987), Expect = 0.0e+00
Identity = 582/676 (86.09%), Postives = 618/676 (91.42%), Query Frame = 0

Query: 1   MACTSVAISNSPAASMAKDIIFKEYNGLRRSHSSNDLRERACMRRSHSDNHICYSANRIH 60
           MACTSVAI NS A SM KDIIFKEYNG+RRSHSS D+RER C++RS+SDNHICYSAN+IH
Sbjct: 1   MACTSVAIGNSSATSMTKDIIFKEYNGIRRSHSSKDIRERTCIKRSYSDNHICYSANKIH 60

Query: 61  ATSTQPKPK-NNNNNNSMGMGIFPLKFSGSFLPNSIRSFLFDMEEPSKDLSSEEEVTKRA 120
           ATSTQPKPK NNNNNNSMGM IFPLKFSGSFLP+ IRSFLFDMEE SKDLSSEEEVT+RA
Sbjct: 61  ATSTQPKPKNNNNNNNSMGMRIFPLKFSGSFLPHPIRSFLFDMEETSKDLSSEEEVTRRA 120

Query: 121 NWIERLLQIRSHWRIKQQKG-GIEIYADHDESGESFCGGDDGGCEVDYYDSEDEGDLSFD 180
           NWIERLL+IR HWR KQ+KG   +IY  HDE+GE FCG DDGGCEVDYYDSEDEG+ SFD
Sbjct: 121 NWIERLLEIRRHWRTKQKKGVENDIYTHHDETGERFCGEDDGGCEVDYYDSEDEGEFSFD 180

Query: 181 TESFARFLVRVPLYDTKVFSQLAFLSNMAYVIPEIKAEDLERYYGLQFVTSSLRKKADAA 240
           TESFARFLV+VPL DTKVFSQLAFLSNMAYVIPEIKAE+LER  GL+FVTSSLRKKA+ A
Sbjct: 181 TESFARFLVKVPLSDTKVFSQLAFLSNMAYVIPEIKAEELERCCGLKFVTSSLRKKANGA 240

Query: 241 AINIKEKLDQHSTCISDEAFDVAESCSVEAMELERTQQIPTTVAYEIAATAASYAHSRAK 300
           A N+KEK DQHSTCISDEA +V E CS EA++LERTQQIPTTVAYEIAATAASY HSRAK
Sbjct: 241 AFNMKEKQDQHSTCISDEALNVGEVCSGEALQLERTQQIPTTVAYEIAATAASYMHSRAK 300

Query: 301 NPSSHPSEPQGKGESSTRAYNPEVAAYVAASTMTAVVAAEEVQKQETAKDLQSLHSSPCE 360
           NPSSHP EPQ +GESS RAYNPEVAAYVAASTMTAVVAAEEVQKQETAK LQSLHSSPCE
Sbjct: 301 NPSSHPLEPQERGESSCRAYNPEVAAYVAASTMTAVVAAEEVQKQETAKGLQSLHSSPCE 360

Query: 361 WFVCDDTNTLTRCFIIQGSDSLASWQANLFFEPAKFEGTDVLVHRGIYEAAKGIYKQFMP 420
           WFVCDD ++ TR FIIQGSDSLASWQANLFFEP KFEG+DVLVHRGIYEAAKGIYKQF+P
Sbjct: 361 WFVCDDIHSSTRYFIIQGSDSLASWQANLFFEPTKFEGSDVLVHRGIYEAAKGIYKQFIP 420

Query: 421 EIIDHLKKYANHAKFQFTGHSLGGSLSLLVHLMLLKNGIVKPSMLQPVVTFGSPFVFCGG 480
           EII+HLK Y  HAKFQFTGHSLGGSLSLLVHLMLLKN IVKP MLQPVVTFGSPFVFCGG
Sbjct: 421 EIIEHLKTYGTHAKFQFTGHSLGGSLSLLVHLMLLKNEIVKPCMLQPVVTFGSPFVFCGG 480

Query: 481 HKILNELGLVEDDIHCVIMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLY 540
           HKIL ELGL EDDI CVIMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLY
Sbjct: 481 HKILIELGLDEDDIRCVIMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLY 540

Query: 541 SPLGKLFILQPDERSSPPHPMLPQGSALYTLDSTQYGYSKGLLRAFLNCPHPLETLSDPT 600
           SP+GK+FILQP+E SSPPHPMLP+GSALYTLDSTQ GYS G+LRAFLNCPHPLETLSDPT
Sbjct: 541 SPMGKIFILQPEEMSSPPHPMLPEGSALYTLDSTQNGYSTGILRAFLNCPHPLETLSDPT 600

Query: 601 AYGSEGTILRDHDSSFYLKALNGVLKQHTNMIVGKVKKQRKLLWPLLASPSPELWSHEGN 660
           AYGSEGTILRDHDSSFYLKALNGVL QHT M  GKV+KQRKLL PLL SPSPELW H+GN
Sbjct: 601 AYGSEGTILRDHDSSFYLKALNGVLNQHTKMHTGKVRKQRKLLLPLLTSPSPELWRHDGN 660

Query: 661 LDNNNNLLSNEIMTGV 675
           LDN N+L+SNEIMTGV
Sbjct: 661 LDNKNSLVSNEIMTGV 676

BLAST of Tan0000306 vs. ExPASy TrEMBL
Match: A0A6J1JUY6 (uncharacterized protein LOC111488580 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111488580 PE=4 SV=1)

HSP 1 Score: 1155.2 bits (2987), Expect = 0.0e+00
Identity = 582/676 (86.09%), Postives = 618/676 (91.42%), Query Frame = 0

Query: 1   MACTSVAISNSPAASMAKDIIFKEYNGLRRSHSSNDLRERACMRRSHSDNHICYSANRIH 60
           MACTSVAI NS A SM KDIIFKEYNG+RRSHSS D+RER C++RS+SDNHICYSAN+IH
Sbjct: 34  MACTSVAIGNSSATSMTKDIIFKEYNGIRRSHSSKDIRERTCIKRSYSDNHICYSANKIH 93

Query: 61  ATSTQPKPK-NNNNNNSMGMGIFPLKFSGSFLPNSIRSFLFDMEEPSKDLSSEEEVTKRA 120
           ATSTQPKPK NNNNNNSMGM IFPLKFSGSFLP+ IRSFLFDMEE SKDLSSEEEVT+RA
Sbjct: 94  ATSTQPKPKNNNNNNNSMGMRIFPLKFSGSFLPHPIRSFLFDMEETSKDLSSEEEVTRRA 153

Query: 121 NWIERLLQIRSHWRIKQQKG-GIEIYADHDESGESFCGGDDGGCEVDYYDSEDEGDLSFD 180
           NWIERLL+IR HWR KQ+KG   +IY  HDE+GE FCG DDGGCEVDYYDSEDEG+ SFD
Sbjct: 154 NWIERLLEIRRHWRTKQKKGVENDIYTHHDETGERFCGEDDGGCEVDYYDSEDEGEFSFD 213

Query: 181 TESFARFLVRVPLYDTKVFSQLAFLSNMAYVIPEIKAEDLERYYGLQFVTSSLRKKADAA 240
           TESFARFLV+VPL DTKVFSQLAFLSNMAYVIPEIKAE+LER  GL+FVTSSLRKKA+ A
Sbjct: 214 TESFARFLVKVPLSDTKVFSQLAFLSNMAYVIPEIKAEELERCCGLKFVTSSLRKKANGA 273

Query: 241 AINIKEKLDQHSTCISDEAFDVAESCSVEAMELERTQQIPTTVAYEIAATAASYAHSRAK 300
           A N+KEK DQHSTCISDEA +V E CS EA++LERTQQIPTTVAYEIAATAASY HSRAK
Sbjct: 274 AFNMKEKQDQHSTCISDEALNVGEVCSGEALQLERTQQIPTTVAYEIAATAASYMHSRAK 333

Query: 301 NPSSHPSEPQGKGESSTRAYNPEVAAYVAASTMTAVVAAEEVQKQETAKDLQSLHSSPCE 360
           NPSSHP EPQ +GESS RAYNPEVAAYVAASTMTAVVAAEEVQKQETAK LQSLHSSPCE
Sbjct: 334 NPSSHPLEPQERGESSCRAYNPEVAAYVAASTMTAVVAAEEVQKQETAKGLQSLHSSPCE 393

Query: 361 WFVCDDTNTLTRCFIIQGSDSLASWQANLFFEPAKFEGTDVLVHRGIYEAAKGIYKQFMP 420
           WFVCDD ++ TR FIIQGSDSLASWQANLFFEP KFEG+DVLVHRGIYEAAKGIYKQF+P
Sbjct: 394 WFVCDDIHSSTRYFIIQGSDSLASWQANLFFEPTKFEGSDVLVHRGIYEAAKGIYKQFIP 453

Query: 421 EIIDHLKKYANHAKFQFTGHSLGGSLSLLVHLMLLKNGIVKPSMLQPVVTFGSPFVFCGG 480
           EII+HLK Y  HAKFQFTGHSLGGSLSLLVHLMLLKN IVKP MLQPVVTFGSPFVFCGG
Sbjct: 454 EIIEHLKTYGTHAKFQFTGHSLGGSLSLLVHLMLLKNEIVKPCMLQPVVTFGSPFVFCGG 513

Query: 481 HKILNELGLVEDDIHCVIMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLY 540
           HKIL ELGL EDDI CVIMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLY
Sbjct: 514 HKILIELGLDEDDIRCVIMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLY 573

Query: 541 SPLGKLFILQPDERSSPPHPMLPQGSALYTLDSTQYGYSKGLLRAFLNCPHPLETLSDPT 600
           SP+GK+FILQP+E SSPPHPMLP+GSALYTLDSTQ GYS G+LRAFLNCPHPLETLSDPT
Sbjct: 574 SPMGKIFILQPEEMSSPPHPMLPEGSALYTLDSTQNGYSTGILRAFLNCPHPLETLSDPT 633

Query: 601 AYGSEGTILRDHDSSFYLKALNGVLKQHTNMIVGKVKKQRKLLWPLLASPSPELWSHEGN 660
           AYGSEGTILRDHDSSFYLKALNGVL QHT M  GKV+KQRKLL PLL SPSPELW H+GN
Sbjct: 634 AYGSEGTILRDHDSSFYLKALNGVLNQHTKMHTGKVRKQRKLLLPLLTSPSPELWRHDGN 693

Query: 661 LDNNNNLLSNEIMTGV 675
           LDN N+L+SNEIMTGV
Sbjct: 694 LDNKNSLVSNEIMTGV 709

BLAST of Tan0000306 vs. TAIR 10
Match: AT3G61680.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 636.0 bits (1639), Expect = 3.5e-182
Identity = 366/691 (52.97%), Postives = 462/691 (66.86%), Query Frame = 0

Query: 1   MACTSVAISNSPAASMAKDIIFKEYNGLRRSHSSNDL-RERACMRRSHSDNHICYSA--- 60
           MA  +   S SPAA  A D++ +E+ GLRRS S  DL  +   +RRS SDNH+C  +   
Sbjct: 1   MAFNTAMASTSPAA--ANDVL-REHIGLRRSLSGQDLVLKGGGIRRSSSDNHLCCRSGNN 60

Query: 61  -NRIHATSTQPKPKNNNNNNSMGMGIFPLKFSGSFLPNSIRSFLFDMEEPSKDLSSEE-- 120
            NRI A S +P  K      S  +G+F  + S S +P+ I++ LF+ +    +  S+E  
Sbjct: 61  NNRILAVSVRPGMK-----TSRSVGVFSFQISSSIIPSPIKTLLFETDTSQDEQESDEIE 120

Query: 121 -------EVTKRANWIERLLQIRSHWRIKQ--QKGGIEIYADHDESGESFCG-GDDGGCE 180
                  +  K+ANW+ERLL+IR  W+ +Q  + G  ++    +ES +  CG  ++ GC 
Sbjct: 121 IETEPNLDGAKKANWVERLLEIRRQWKREQKTESGNSDV---AEESVDVTCGCEEEEGCI 180

Query: 181 VDYYDSEDEGDLSFDTESFARFLVRVPLYDTKVFSQLAFLSNMAYVIPEIKAEDLERYYG 240
            +Y      GD  +  ESF+R LV+V   + K  SQLA+L N+AY IPEIK EDL R YG
Sbjct: 181 ANY--GSVNGD--WGRESFSRLLVKVSWSEAKKLSQLAYLCNLAYTIPEIKGEDLRRNYG 240

Query: 241 LQFVTSSLRKKADAAAINIKEKLDQHSTCISDEAFDVAESCSVEAMELERTQQIPTTVAY 300
           L+FVTSSL KKA AA   ++EKL+Q  T +      V  S  +E+ +  +     +  AY
Sbjct: 241 LKFVTSSLEKKAKAAI--LREKLEQDPTHV-----PVITSPDLESEKQSQRSASSSASAY 300

Query: 301 EIAATAASYAHSRAKNPSSHPSEPQGKGESSTRAYNPEVAAYVAASTMTAVVAAEEVQKQ 360
           +IAA+AASY HS  +   S P             Y    AA  AASTMTAVVAA E +K 
Sbjct: 301 KIAASAASYIHSCKEYDLSEP------------IYKSAAAAQAAASTMTAVVAAGEEEKL 360

Query: 361 ETAKDLQSLHSSPCEWFVCDDTNTLTRCFIIQGSDSLASWQANLFFEPAKFEGTDVLVHR 420
           E A++LQSL SSPCEWFVCDD NT TRCF+IQGSDSLASW+ANLFFEP KFE TDVLVHR
Sbjct: 361 EAARELQSLQSSPCEWFVCDDPNTYTRCFVIQGSDSLASWKANLFFEPTKFEDTDVLVHR 420

Query: 421 GIYEAAKGIYKQFMPEIIDHLKKYANHAKFQFTGHSLGGSLSLLVHLMLLKNGIVKPSML 480
           GIYEAAKGIY+QF+PEI +HL ++ + AKFQFTGHSLGGSLSL+V+LML+  G+V    +
Sbjct: 421 GIYEAAKGIYEQFLPEITEHLSRHGDRAKFQFTGHSLGGSLSLIVNLMLISRGLVSSEAM 480

Query: 481 QPVVTFGSPFVFCGGHKILNELGLVEDDIHCVIMHRDIVPRAFSCNYPNHVAAVLKRLSG 540
           + VVTFGSPFVFCGG KIL ELGL E  +HCV+MHRDIVPRAFSCNYP+HVA VLKRL+G
Sbjct: 481 KSVVTFGSPFVFCGGEKILAELGLDESHVHCVMMHRDIVPRAFSCNYPDHVALVLKRLNG 540

Query: 541 SFRSHSCLNKSKLLYSPLGKLFILQPDERSSPPHPMLPQGSALYTLDSTQYGYSKGLLRA 600
           SFR+H CLNK+KLLYSP+GK++ILQP E  SP HP LP G+ALY L+++  GYS   LRA
Sbjct: 541 SFRTHPCLNKNKLLYSPMGKVYILQPSESVSPTHPWLPPGNALYILENSNEGYSPTALRA 600

Query: 601 FLNCPHPLETLSDPTAYGSEGTILRDHDSSFYLKALNGVLKQHTNMIVGKVKKQRKLLWP 660
           FLN PHPLETLS   AYGSEG++LRDHDS  Y+KA+NGVL+QHT +IV K + QR+ +WP
Sbjct: 601 FLNRPHPLETLSQRAAYGSEGSVLRDHDSKNYVKAVNGVLRQHTKLIVRKARIQRRSVWP 649

Query: 661 LLASPSPELWSHEGNLDNNNNLLSNEIMTGV 675
           +L S    L        N +   + EIMT V
Sbjct: 661 VLTSAGRGL--------NESLTTAEEIMTRV 649

BLAST of Tan0000306 vs. TAIR 10
Match: AT1G02660.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 473.0 bits (1216), Expect = 4.0e-133
Identity = 269/594 (45.29%), Postives = 373/594 (62.79%), Query Frame = 0

Query: 78  GMGIFPLKFSGSFLPNSIRSFLFDMEEPSKDLSSEEEVTKRAN--WIERLLQIRSHWRIK 137
           G GI   + SG  L +++     D  +P  + ++ E  T+R N  W+ ++L ++S W+ +
Sbjct: 62  GGGIASRRRSGLCLDDAVLVDSGDSRKPIAEETAVEMDTERRNGSWVLKILDVQSTWKHE 121

Query: 138 QQKGGIEIY---ADHDESGE---SFCGGDDGGCEVDYY---DSEDEGDLSFDTESFARFL 197
           +++   E+     D DE  E   +    DDGGC+V      D  +      D ESF++ L
Sbjct: 122 EEEDDDEVEDEDGDEDEEVELDDAVVSEDDGGCDVCSVLEDDGNEANKFQLDRESFSKLL 181

Query: 198 VRVPLYDTKVFSQLAFLSNMAYVIPEIKAEDLERYYGLQFVTSSLRKKADAAAINIKEKL 257
            RV L ++K+++QL++L N+AY I +IK  +L +YYGL+FVTSS  K   A        L
Sbjct: 182 RRVTLPESKLYAQLSYLGNLAYSISKIKPANLSKYYGLRFVTSSAEKTESA--------L 241

Query: 258 DQHSTCISDEAFDVAES-CSVEAMELERTQQIPTTVAYEIAATAASYAHSRAKN--PSSH 317
              +  +S E   + E+   VE  E  ++++I  + AYEI A+AASY HSR  N  P + 
Sbjct: 242 KAENGEVSGETKPIVEAEEEVEEEEKNKSRKISASAAYEIVASAASYLHSRTNNILPFNS 301

Query: 318 PSEPQGKGESSTRAYNPEVAAYVAASTMTAVVAAEEVQKQETAKDLQSLHSSPCEWFVCD 377
            S+ +   +      N E ++ VA S +T+VVAAEE  KQ  A DL+S  SSPC+WF+CD
Sbjct: 302 SSKAENSDKHDVNLTNAESSSDVAYS-VTSVVAAEEDVKQAVADDLKSTISSPCDWFICD 361

Query: 378 DTNTLTRCFIIQGSDSLASWQANLFFEPAKFEGTDVLVHRGIYEAAKGIYKQFMPEIIDH 437
           D  + TR  +IQGS+SLASWQANL FEP +FEG   +VHRGIYEAAKG+Y+Q +PE+  H
Sbjct: 362 DDQSHTRFVVIQGSESLASWQANLLFEPIEFEGLGAIVHRGIYEAAKGMYEQMLPEVKAH 421

Query: 438 LKKYANHAKFQFTGHSLGGSLSLLVHLMLLKNGIVKPSMLQPVVTFGSPFVFCGGHKILN 497
           +K +   AKF+FTGHSLGGSLSLL++LMLL  G V  S L PV+T+G+PFV CGG ++L 
Sbjct: 422 IKTHGTSAKFRFTGHSLGGSLSLLLNLMLLVRGEVPASSLLPVITYGAPFVLCGGDRLLK 481

Query: 498 ELGLVEDDIHCVIMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSHSCLNKSKLLYSPLGK 557
           +LGL +  +  ++MHRDIVPRAFSCNYP HVA +LK ++G+FRSH CLNK  +LYSP+G+
Sbjct: 482 KLGLPKSHVQAIVMHRDIVPRAFSCNYPYHVAELLKAVNGNFRSHPCLNKQSMLYSPMGE 541

Query: 558 LFILQPDERSSPPHPMLPQGSALYTLDS---------TQYGYSKGLLRAFLNCPHPLETL 617
           L ILQPDE  SP H +LP G+ LY L S         +     +     FLN PHPL+ L
Sbjct: 542 LLILQPDETFSPGHELLPSGNGLYLLTSDFESPDIEDSDEERLRAAQTVFLNTPHPLDIL 601

Query: 618 SDPTAYGSEGTILRDHDSSFYLKALNGVLKQHTNMI-VGKVKKQRKLLWPLLAS 648
           SD +AYGS GTI RDHD + YLKA+  V+++  N I   K + +R L WP+L +
Sbjct: 602 SDRSAYGSSGTIQRDHDMNSYLKAVRSVIRKEVNQIRRAKREHRRSLWWPILVA 646

BLAST of Tan0000306 vs. TAIR 10
Match: AT3G62590.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 432.2 bits (1110), Expect = 7.8e-121
Identity = 250/521 (47.98%), Postives = 328/521 (62.96%), Query Frame = 0

Query: 120 NWIERLLQIRSHWRIKQQKGGIEIYADHDE--------SGESFCGGDDGGCEVDYYDSED 179
           NW+ ++L++ S W+ K+Q+ G     + DE          E  C   D  C +D  D ++
Sbjct: 90  NWVLKILEVGSIWKGKRQRSGGGGGGEEDEEEEVAEPKKKEDLCEECD-FCRIDDDDEDE 149

Query: 180 EGDLSFDTESFARFLVRVPLYDTKVFSQLAFLSNMAYVIPEIKAEDLERYYGLQFVTSSL 239
           E + +     F+  L ++P+ D ++F++L+FL N+AY IP+IK E+L +Y  L+FVTSS+
Sbjct: 150 EKEKT--VFEFSEMLSKIPVEDAQMFAKLSFLGNLAYSIPKIKPENLLKYQKLRFVTSSI 209

Query: 240 RKKADAAAINIKEKLDQHSTCISDEAFDVAESCSVEAMELERTQQIPTTVAYEIAATAAS 299
            K+       +  K+++++    DE               E+ + I   VAY IAA+AAS
Sbjct: 210 EKR-------MSLKVEENNNGEEDE---------------EKKKLINPAVAYRIAASAAS 269

Query: 300 --YAHSRAKNPSSHPSEPQGKGESSTRAYNPEVAAYVAASTMTAVVAAEEVQKQETAKDL 359
             ++HS++  P            SS R  N E +    A ++TAVVAA+E  KQ  A DL
Sbjct: 270 RLFSHSKSVLPFG----------SSKRQDNEEASLLATADSVTAVVAAKEEVKQAVADDL 329

Query: 360 QSLHSSPCEWFVCDDTNTLTRCFIIQGSDSLASWQANLFFEPAKFEGTDVLVHRGIYEAA 419
           +S  S PCEWFVCDD  + TR F IQGSDSLASWQANL FEP  FE  DVLVHRGIYEAA
Sbjct: 330 KSNRSPPCEWFVCDDDKSGTRFFFIQGSDSLASWQANLLFEPVPFEDLDVLVHRGIYEAA 389

Query: 420 KGIYKQFMPEIIDHL-KKYANHAKFQFTGHSLGGSLSLLVHLMLLKNGIVKPSMLQPVVT 479
           KGIY+Q +PE+  HL  +  N A  +F+GHSLGGSLSLLV+LMLL  G V  S L PV+T
Sbjct: 390 KGIYEQMLPEVHAHLNSRGKNRAFLRFSGHSLGGSLSLLVNLMLLIRGQVPASSLLPVIT 449

Query: 480 FGSPFVFCGGHKILNELGLVEDDIHCVIMHRDIVPRAFSCNYPNHVAAVLKRLSGSFRSH 539
           FGSP + CGG ++L +LGL +  +  + MHRDIVPRAFSCNYPN  A +LK L+G+FR+H
Sbjct: 450 FGSPCIMCGGDRLLQKLGLPKSHLLGISMHRDIVPRAFSCNYPNRAAKLLKALNGNFRNH 509

Query: 540 SCLNKSKLLYSPLGKLFILQPDERSSPPHPMLPQGSALYTLDSTQYGYSKGLLRA----F 599
            CLN   +LYSP+GKL ILQP ER SPPHP+LP GS LY L S     ++  LRA    F
Sbjct: 510 PCLNNQNVLYSPMGKLLILQPSERFSPPHPLLPPGSGLYLLASKNTDETEKSLRAAKILF 569

Query: 600 LNCPHPLETLSDPTAYGSEGTILRDHDSSFYLKALNGVLKQ 626
            N PHPLE LSD  +YGSEG I R+HD S YLKAL  V+++
Sbjct: 570 FNSPHPLEILSDRRSYGSEGKIKRNHDMSSYLKALRHVIRK 575

BLAST of Tan0000306 vs. TAIR 10
Match: AT3G14360.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 43.9 bits (102), Expect = 6.0e-04
Identity = 40/137 (29.20%), Postives = 61/137 (44.53%), Query Frame = 0

Query: 378 DSLASWQANLFFEPAKFEGTDV-----LVHRGIYEAAKGIYKQFMPEIIDHLKKYANHAK 437
           D   ++  NLF + +  E         +V R  Y A + I K+ + E          +A+
Sbjct: 281 DDTTTFHYNLFEQTSSEEENSKKNLLDMVERSAYYAVRVILKRLLSE--------HENAR 340

Query: 438 FQFTGHSLGGSLSLLVHLMLLKNGIVK-PSMLQPVVTFGSPFVFCGGHKI----LNELGL 497
           F  TGHSLGG+L++L   +L+ N   +    L  V TFG P +  G  ++      +L  
Sbjct: 341 FVVTGHSLGGALAILFPTLLVLNEETEIMKRLLGVYTFGQPRI--GNREVGLFMKAQLNQ 400

Query: 498 VEDDIHCVIMHRDIVPR 505
             D    V+   DIVPR
Sbjct: 401 PVDRYFRVVYCNDIVPR 407