Spg039975 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg039975
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptionreceptor homology region, transmembrane domain- and RING domain-containing protein 2-like
Locationscaffold10: 46970633 .. 46976552 (-)
RNA-Seq ExpressionSpg039975
SyntenySpg039975
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTGCATTTTCCTCTTTATCGTTATCATGTATTTTATTTTTGTTTTCATTGTTCGTTTCCGTTTTCTGGTCGGAGTAGGCAAATTCAATCTCGAGAGTTTTATCTCACAGCGAATCAACGAAACGAAGATTTTCCTTCTGAGAGATGGAAGCTGATGTTTCTATTGATATTTCTAAACCCTGATAATTCTGTTTTCCGTTTCAATCTCCATCCTCTCCGTTTTCTTTCTCACTGATTAGGGTTTTGCGCTCCATGGCTTTGAGTGCTATGAATCGAGGTCTCGCGTTATTGTTTTCACTTCTTTTGGTTGGTTGTTGCGGCTCTGCAGCTAATGTGGTATTGATTGGGACCAACGTTACTCTTTCATTCGACTCCATTGAAGCTAATTTCGGTTAGTTTTCTTTATGCTCTCTTTTTCCGCCCTTGCCTGCTCGATTCATTTGAAAATTTTGATAGGTAAATTGTCTTGTGTACTAGTGAATGCCTTCTGAGTCGGGCACTGGATTAAATGTCTATCCTCTCGTCATCACTAGTCGCCGTGAATTGTGTCAATGGGCGATATGTTTGACTCTTTTGATTAAATGCCTTCTGATTCTTTTGTTCGTAAGGAATAATTTTATGATTCAAGATTCTGTCAAATAGTAATTTAGGATGCTATTATGTTTTGAGAAGATAAATCGGATATGATTATTCAAGAGGGAGACGAGTCAAATATATACAATACAGACTGCTACATGGGTTCAGATTTATTTTCTACGGATATTTTTTTCTTTTTTAATTGCCATAATTGACTCTGTAATTTATTTCTGCCTCTGGTATTTCTGATTTCAGCTCCTACAATAGAAAGTTCAGGGGAACCTGGGGTGTTGTACCTGGCAAAACCTCTTGATGCATGCTCGACATTGGAAAATAAAGTTCGGCTGCCTGTAAACTCTAGTTCACCATTTGCGCTTATAATTAGAGGAGGATGTAGCTTCGAGGATAAAGTGAGGAGAGCCCAAGTGGCTGGATTTAAAGCTGCAATTGTCTATGACAATGAAGACAGTGGTTCCCTTATTGCGAGTAATGACTTTCTACACTATTAACTACATGTCGATTCCCCATTTATGGAAGTGGATGACTTTCTAGACGATTTATAGATTTTAACTATGCCTTTGTTGCTATGCATGCTGCGGCGATTCAATTTACTTGTAGTGTAGATATTAGTGCTTTAATGATCTACTTATCTACTTTCAAAGATTGAATTAAATTTAGTCATTACAAATATTTTCTTAAAAAAACTATACTTCAAAGACTTGCAGAATTTTATTTTAAATTTTTTTTTGGAGAAATTGACTGAGCAGTTGTCGATCCAAGATCTTCACTTAAAACCCAAGCCTTTCTTCTAAAAGGTCTTGTCTAGAAGAACCCAAACAACATGATCAAATATCTTTCAAGTTAATTTTCAATCAGTTTTCCTTGGTGTTAGTGTATGGAATGGTGTTGGTCTTGGTATATCTTAGCTTCATATTTTTGAAATAAATGATGAACTTGAAAATATCAAATTACTTGCTCATCGATTAGAATACTCATCTAATTCTTGAATTACGGTTCTTTATTACCACGGATTCAGCAAACAAAGTTTGTACGTATGCTATGGGAATCACTGGAATCGGAATCCTGAATGTAGGCTATCTTGTGGTTGGTGGCTAAGATTGTAGCACTTGTTTGGCATCTTGATGTTATTGCTTTATCAATCTAAACTTTATGCCCATTCTTGATTTTAAATTAAAAGTTTTACACTTCTACTTTTCTCTGGTTTTTCTACTCTGTTAATGCGTATGGAGTTATTTGGCCAACATTTATCGTATGAATGCATAAGAGTAAATGTTTTGAAAAGTGAGCTGCTTTACGCACGGTTCTACAAAGAAAAATTTTCTTGTTAGCTTGAAGATTTCCTTGTCATAACTTTGAAGATGTTGCAGTGGCAGGGAGCTCAGCTGGTATTAGAATACATGCTGTATTTGTTACTAAATCATCCGGTGAGACATTGAAGAAGTACGCAGGCTTGGCTAATGTTGAGATATGGATAGTTCCAAGCTTTGAAAACTCTGCATGGTCCATCATGGCTATCTCCTTTATTTCTCTTCTTGCAATGTCTGCAGTGCTTGCTACTTGTTTCTTCGTTCGTAGGCATCGAATAAGACGAGAACGTCCTCGATCTTCTCATTCTCAAGAGTTTCGTGGCATGAGCAGACGTGTTGTAAAAGCAATGCCAAGCCTGATATTTACTACTGCTTTAGAGGATAATTGTACATCAGTAACATGTGCCATATGCCTTGAGGACTATACTGCTGGTGAGAAGCTCAGAATTCTACCATGTCGACACAGTAAGTTCACTCCTTTATTCTTAAGTTTATATTTTCTTTTTATGTATATATCTTGCATTTATAGGAATATTAGTCAATACTTTATGCTGTAGAACCTGTAATGGTGTGGTTGGTCCTTGTTTCTTTTAATTTCCTTTTCATCTATAGCTCAGAAGTCCATTTGTGAATTCAGGTTTATAATAAATGGGCTCTTAATCCTTATAAAGGAAGAAAACTAGCAATAGCATGTTTGTTAATGTAAATCATTAAAGTAGTGTTTAATCAATGTCTTATTAAATCGTTGCCCGAAGACCTAGTTTAAATTGTTCAAAAAATGTTAGAGATACTCTGATTCAATAAAGGAAGTTTAAGCTCTTTATGAACATAAAGTTTTGGCTTCTTTATTTTATTAATGTAACAACCACTTTCACGGCCTGAAGCCATTTGCTTTATTTAGAAAGGTAAAATTTCAACATCATGTTGAAATTTGATTTTGCAAGTACTGTTTTGCCATTAACCCGAAACAAAATTTAATAATTAGAAATAGTTAAGATAGTTTAGAGTAGCTTTGGTAACCCTGGTTATGGCTTGGAAAAGAATGTTCTTAAACCCAGTTAGGCTACTTTGAGGCTAATTTAAGGAAGCAGATATTTGGATGTGGAGGTCTTGAAAAATGGTTTAGATAATTTAGTTGCTGGAGGACTAGGCAGCAGCTCGCTTGCCAGCCGGCAACTAAAATTTGGATCATTGGTTTGTCTCTGTGGTTTAATTAACGTCTGAAGAGTGAAGATATTTCCTAATTTCAACGTTTGGACTAACATAAATGTACTCTTTTTTTAGTTGCAAAGTCCCACCAGCAGGATCAAAGGTTGATTTATATAAATAAAGGGACAGATAGAACTGGGCATAAAAAGATTGCATAGAAGTCGAGAGTGATAAAAATATTCTGCCTTCAGGGGCTTTGGGAGCCTGGTTAACCAACTTCCTATGTATATCTGCAACATATTTTTTGGGGAAAGGAAATCAAACTTCTTCATTGAATTAGCAAAAAGTTGGACAAGAACTTTACTATAATTTTACAAAAAAAGCCCCTCATTTTTTTTTAATTACTAAAGGAGTACAATTTAAAACAAAGTTACAGAAAGAATCACCATAGGAAAAGGAGAACTTAACAAAGTCAAGAACTTCATCCTATTGCCGGTCCTCTCCATAAAATGGAACAATGCCTTCCTAACTATATTCCCTGACATAAGACCTTAACCACATATATCCACAGAATTCTTGACCTTCTAAATCTATTCCTACTCAAGGCCTAAGAAATATTGTCTTTTGCATCTCTGTCAAAGGCCCTGGCTATATGAAATTGCAACATCAAGGATGTCCATCATTCTTAGCTAAAGGTACAGTGAAAAAGATAGATTGCAGCTATCTTTGGATTTCTTTTTTGTGTTCTTTCAGCCATTGTGATACTGCCATGGTCCCTTTCCTTTCAAAATCATGCCTGAGGGGTTTTTTTTTTTTTTTTTTTTTTCTTTGTGGGATGATTTAGATGTTAGTATTTTCCATCTATCATTGAGCTATCTCCATATAGAGTTCCAAATCGTTGTTGAAACTCACGCAATGCTACCTCTGTTAGGTTTCTTCTCAAGACAATGTTCCACCCCCCATTGCTGCCCTCCACATTTCCTTGACACAGGCGCCTCCTTATTCTGTGACACTACAAAAAGTCTAGGAAAAAAATCATTTGGGCTGAGAGCCTGAGACAATTAGCCAGTTATCTCTTGAAAAGTAGTTTATGTGCTCATTTCCAGTTTTAATATAGTAGAGGCTTCAGCCCCAAGGGGCACCTCATTGGCTAAGGTGCGTAGTCTCAAGGTAACACCACCAAGTAACACCTACCTAGAGGTTGCATGTTTGAATATTAGGGTGAGCTTAATAAGGAAAATCTTTAGTGCTGTGAAGAAACTAATATATTCAGATAGGAAGCGATGTAAATAAAATATGGTCCTTGGGTTGGGCACGGACTCTAAAAACTATATATATATTTGCAGAGGCTTCAAAAAGGAGGTCTAAGCTTCATGATACATTTCTATTTTCCGACCATGTATTGCTTAATGTTTTCCTCTGAACATGAGCAGTGAACCATGTTTCTTTCCTCAAATACTTCTTACCACCTGATTCTATAGCTACTATGAATATATACAATAGGAAAGTTCATTTTTCCAACAAAGCCTCATTTCTTTTCATCAGATTTCCATCCCCAGTTCCCCCCACCTTCAACCAGTTACTAGCAAACAGAATTCTAGTAATTTCTTTCGACTCTGTGCTTACAGGATCAAGGTTGCATAGATAATCGTTATGTACTTTTTCCTACTCTTTCTTTTTTGCCATGCAATACTTTGCTTACATGCATACCTTTACCCGCATTTGTTTCATATATATCCTCTAGCTAACCATTTGTGATTTTGGAACTGATTTCCAGAATTTCATGCCTTCTGTGTGGATTCTTGGCTTACTGCTTGGAGAACCTTCTGCCCGGTCTGCAAGCGAGATGCAAGAACTAGCACTGGTGATCCACCACCTTCAGAATCCACACCATTGCTTTCAAGCACTCCCAACTCTGTTGCTTCCTCTTCAATATTATCTTCTGTGAGATCATCATTACCGCCAAGCTCTGCCATACAAATATCCCCATCATCAAGATCGCCTTCGGTATCTCGGAACCACTCTCTCTCAAGCACCCCTTACCATCAGCAGTCTCTTAGATCCTCCTACCACCAATCTCCATCCTTGAGCGTGAGCAGAAGTTCAGTTGAACTCAGAAATGCATCTTCCCAAAGATCTTATGCTTCTAATTTGGTTTCGCCGTACTCCTCGTGTTACCCATCCATGTCACCTTTACATTCAAGATACATGATTCCGCTTATTCCGAGTCCAAGCAATGCCTCACCAAGCATCTTTGGTTCTTCCAGTCATCAACAGAATCCCTTGCATATTAGCGGGTCAGCTGCAAGTTTTTCTCCATTCGCTTCAGCTCGGTCTCTTCCTGAATGCTGATACGGGAGCGTCGAGAATCTTTCGTGCAGAGATATAGCCGTCCCTCTTTTCGTAGAGGATCGGGGGGTCGGGTGAGGTATGGTTGAATTATGGCATCATCTAGTCTGTAAATCGATTCCTTAGTCGTTGATCGTGTCTGAAATACCCTGGTTGGCCGGCATCATTGTATATTATTTCATACGATTGCTCCATTTCTTTTGGCTTTGAAGTTCCATTTGTGGTACTTGCTGTAGAGGTTGTTAAAATTTTAAAGTGAAGCAGAGTATATACTTCTGGGGTGGGTTTGAAATTTTTTTTTAATGGATTGTTGGGATATAGATAAATTGGACGCTTAGTTTAACAGCTAACCAGGAAGATTTGTAATATACCGCTTCTCTTCTCTCCTCATCCCTTCTTGGAGGCCCCATGGATCCGTTTAACACAATTAGTTGAAAAGGGGAGCTTCTGTTTTTCCACATGCGTATTTGTCTACTTTTTTCTCTTCCCACCAGATTATTGTTTATATTGAATTCTCAC

mRNA sequence

ATGGCTTTGAGTGCTATGAATCGAGGTCTCGCGTTATTGTTTTCACTTCTTTTGGTTGGTTGTTGCGGCTCTGCAGCTAATGTGGTATTGATTGGGACCAACGTTACTCTTTCATTCGACTCCATTGAAGCTAATTTCGCTCCTACAATAGAAAGTTCAGGGGAACCTGGGGTGTTGTACCTGGCAAAACCTCTTGATGCATGCTCGACATTGGAAAATAAAGTTCGGCTGCCTGTAAACTCTAGTTCACCATTTGCGCTTATAATTAGAGGAGGATGTAGCTTCGAGGATAAAGTGAGGAGAGCCCAAGTGGCTGGATTTAAAGCTGCAATTGTCTATGACAATGAAGACAGTGGTTCCCTTATTGCGATGGCAGGGAGCTCAGCTGGTATTAGAATACATGCTGTATTTGTTACTAAATCATCCGGTGAGACATTGAAGAAGTACGCAGGCTTGGCTAATGTTGAGATATGGATAGTTCCAAGCTTTGAAAACTCTGCATGGTCCATCATGGCTATCTCCTTTATTTCTCTTCTTGCAATGTCTGCAGTGCTTGCTACTTGTTTCTTCGTTCGTAGGCATCGAATAAGACGAGAACGTCCTCGATCTTCTCATTCTCAAGAGTTTCGTGGCATGAGCAGACGTGTTGTAAAAGCAATGCCAAGCCTGATATTTACTACTGCTTTAGAGGATAATTGTACATCAGTAACATGTGCCATATGCCTTGAGGACTATACTGCTGGTGAGAAGCTCAGAATTCTACCATGTCGACACAGCCCTGGCTATATGAAATTGCAACATCAAGGATGTCCATCATTCTTAGCTAAAGAATTTCATGCCTTCTGTGTGGATTCTTGGCTTACTGCTTGGAGAACCTTCTGCCCGGTCTGCAAGCGAGATGCAAGAACTAGCACTGGTGATCCACCACCTTCAGAATCCACACCATTGCTTTCAAGCACTCCCAACTCTGTTGCTTCCTCTTCAATATTATCTTCTGTGAGATCATCATTACCGCCAAGCTCTGCCATACAAATATCCCCATCATCAAGATCGCCTTCGGTATCTCGGAACCACTCTCTCTCAAGCACCCCTTACCATCAGCAGTCTCTTAGATCCTCCTACCACCAATCTCCATCCTTGAGCGTGAGCAGAAGTTCAGTTGAACTCAGAAATGCATCTTCCCAAAGATCTTATGCTTCTAATTTGGTTTCGCCGTACTCCTCGTGTTACCCATCCATGTCACCTTTACATTCAAGATACATGATTCCGCTTATTCCGAGTCCAAGCAATGCCTCACCAAGCATCTTTGGTTCTTCCAGTCATCAACAGAATCCCTTGCATATTAGCGGGTCAGCTGCAAGTTTTTCTCCATTCGCTTCAGCTCGGTCTCTTCCTGAATGCTGA

Coding sequence (CDS)

ATGGCTTTGAGTGCTATGAATCGAGGTCTCGCGTTATTGTTTTCACTTCTTTTGGTTGGTTGTTGCGGCTCTGCAGCTAATGTGGTATTGATTGGGACCAACGTTACTCTTTCATTCGACTCCATTGAAGCTAATTTCGCTCCTACAATAGAAAGTTCAGGGGAACCTGGGGTGTTGTACCTGGCAAAACCTCTTGATGCATGCTCGACATTGGAAAATAAAGTTCGGCTGCCTGTAAACTCTAGTTCACCATTTGCGCTTATAATTAGAGGAGGATGTAGCTTCGAGGATAAAGTGAGGAGAGCCCAAGTGGCTGGATTTAAAGCTGCAATTGTCTATGACAATGAAGACAGTGGTTCCCTTATTGCGATGGCAGGGAGCTCAGCTGGTATTAGAATACATGCTGTATTTGTTACTAAATCATCCGGTGAGACATTGAAGAAGTACGCAGGCTTGGCTAATGTTGAGATATGGATAGTTCCAAGCTTTGAAAACTCTGCATGGTCCATCATGGCTATCTCCTTTATTTCTCTTCTTGCAATGTCTGCAGTGCTTGCTACTTGTTTCTTCGTTCGTAGGCATCGAATAAGACGAGAACGTCCTCGATCTTCTCATTCTCAAGAGTTTCGTGGCATGAGCAGACGTGTTGTAAAAGCAATGCCAAGCCTGATATTTACTACTGCTTTAGAGGATAATTGTACATCAGTAACATGTGCCATATGCCTTGAGGACTATACTGCTGGTGAGAAGCTCAGAATTCTACCATGTCGACACAGCCCTGGCTATATGAAATTGCAACATCAAGGATGTCCATCATTCTTAGCTAAAGAATTTCATGCCTTCTGTGTGGATTCTTGGCTTACTGCTTGGAGAACCTTCTGCCCGGTCTGCAAGCGAGATGCAAGAACTAGCACTGGTGATCCACCACCTTCAGAATCCACACCATTGCTTTCAAGCACTCCCAACTCTGTTGCTTCCTCTTCAATATTATCTTCTGTGAGATCATCATTACCGCCAAGCTCTGCCATACAAATATCCCCATCATCAAGATCGCCTTCGGTATCTCGGAACCACTCTCTCTCAAGCACCCCTTACCATCAGCAGTCTCTTAGATCCTCCTACCACCAATCTCCATCCTTGAGCGTGAGCAGAAGTTCAGTTGAACTCAGAAATGCATCTTCCCAAAGATCTTATGCTTCTAATTTGGTTTCGCCGTACTCCTCGTGTTACCCATCCATGTCACCTTTACATTCAAGATACATGATTCCGCTTATTCCGAGTCCAAGCAATGCCTCACCAAGCATCTTTGGTTCTTCCAGTCATCAACAGAATCCCTTGCATATTAGCGGGTCAGCTGCAAGTTTTTCTCCATTCGCTTCAGCTCGGTCTCTTCCTGAATGCTGA

Protein sequence

MALSAMNRGLALLFSLLLVGCCGSAANVVLIGTNVTLSFDSIEANFAPTIESSGEPGVLYLAKPLDACSTLENKVRLPVNSSSPFALIIRGGCSFEDKVRRAQVAGFKAAIVYDNEDSGSLIAMAGSSAGIRIHAVFVTKSSGETLKKYAGLANVEIWIVPSFENSAWSIMAISFISLLAMSAVLATCFFVRRHRIRRERPRSSHSQEFRGMSRRVVKAMPSLIFTTALEDNCTSVTCAICLEDYTAGEKLRILPCRHSPGYMKLQHQGCPSFLAKEFHAFCVDSWLTAWRTFCPVCKRDARTSTGDPPPSESTPLLSSTPNSVASSSILSSVRSSLPPSSAIQISPSSRSPSVSRNHSLSSTPYHQQSLRSSYHQSPSLSVSRSSVELRNASSQRSYASNLVSPYSSCYPSMSPLHSRYMIPLIPSPSNASPSIFGSSSHQQNPLHISGSAASFSPFASARSLPEC
Homology
BLAST of Spg039975 vs. NCBI nr
Match: XP_022939223.1 (receptor homology region, transmembrane domain- and RING domain-containing protein 2-like [Cucurbita moschata])

HSP 1 Score: 777.7 bits (2007), Expect = 5.6e-221
Identity = 418/467 (89.51%), Postives = 429/467 (91.86%), Query Frame = 0

Query: 1   MALSAMNRGLALLFSLLLVGCCGSAANVVLIGTNVTLSFDSIEANFAPTIESSGEPGVLY 60
           MALSAMN GLALLFSLLLVG CG AANVVLIGTNVTLSFDS+EANFAP I S GEPGVLY
Sbjct: 1   MALSAMNPGLALLFSLLLVGSCGCAANVVLIGTNVTLSFDSVEANFAPAIGSPGEPGVLY 60

Query: 61  LAKPLDACSTLENKVRLPVNSSSPFALIIRGGCSFEDKVRRAQVAGFKAAIVYDNEDSGS 120
           LAKPLDACSTLE+   LPVNSSSPF LIIRGGCSFEDKVRRAQ+AGFKAAI+YDNED G 
Sbjct: 61  LAKPLDACSTLESNTALPVNSSSPFVLIIRGGCSFEDKVRRAQMAGFKAAIIYDNEDGGG 120

Query: 121 LIAMAGSSAGIRIHAVFVTKSSGETLKKYAGLANVEIWIVPSFENSAWSIMAISFISLLA 180
           LIAMAGSSAGIRIHAVFVTKSSGETLKKYAGLANVEIWIVPSFENSAWSIMAISFISLLA
Sbjct: 121 LIAMAGSSAGIRIHAVFVTKSSGETLKKYAGLANVEIWIVPSFENSAWSIMAISFISLLA 180

Query: 181 MSAVLATCFFVRRHRIRRERPRSSHSQEFRGMSRRVVKAMPSLIFTTALEDNCTSVTCAI 240
           MSAVLATCFFVRRHRIRR RPRSSH+QEFRGMSRRVVKAMPSLIFTTALEDNCTSVTCAI
Sbjct: 181 MSAVLATCFFVRRHRIRRGRPRSSHAQEFRGMSRRVVKAMPSLIFTTALEDNCTSVTCAI 240

Query: 241 CLEDYTAGEKLRILPCRHSPGYMKLQHQGCPSFLAKEFHAFCVDSWLTAWRTFCPVCKRD 300
           CLEDYTAGEKLRILPCRH                  +FHA CVDSWLTAWRTFCPVCKRD
Sbjct: 241 CLEDYTAGEKLRILPCRH------------------KFHALCVDSWLTAWRTFCPVCKRD 300

Query: 301 ARTSTGDPPPSESTPLLSSTPNSVASSSILSSVRSSLPPSSAIQISPSSRSPSVSRNHSL 360
           ARTSTGDPPPSESTPLLSS P+SVASSSILSSVRSSLPPSSAIQISPSSRSPSVSRNHSL
Sbjct: 301 ARTSTGDPPPSESTPLLSSAPSSVASSSILSSVRSSLPPSSAIQISPSSRSPSVSRNHSL 360

Query: 361 SSTPYHQQSLRSSYHQSPSLSVSRSSVELRNASSQRSYASNLVSPYSSCYPSMSPLHSRY 420
           SSTPY QQSLRSSYHQ+ SLS+SRSSVELRNASSQRSYAS+LVSPYSSCYPSMSPLHSRY
Sbjct: 361 SSTPYLQQSLRSSYHQASSLSISRSSVELRNASSQRSYASHLVSPYSSCYPSMSPLHSRY 420

Query: 421 MIPLIPSPSNASPSIFGSSSHQQNPLHISGSAASFSPFASARSLPEC 468
           MIPLIPSPSNASPSI GSSSHQ NPLH SGSAASFSPFASA+SLPEC
Sbjct: 421 MIPLIPSPSNASPSIIGSSSHQHNPLHCSGSAASFSPFASAQSLPEC 449

BLAST of Spg039975 vs. NCBI nr
Match: KAG6578333.1 (Receptor-likey region, transmembrane domain- and RING domain-containing protein 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 777.7 bits (2007), Expect = 5.6e-221
Identity = 418/467 (89.51%), Postives = 429/467 (91.86%), Query Frame = 0

Query: 1   MALSAMNRGLALLFSLLLVGCCGSAANVVLIGTNVTLSFDSIEANFAPTIESSGEPGVLY 60
           MALSAMN GLALLFSLLLVG CG AANVVLIGTNVTLSFDS+EANFAP I S GEPGVLY
Sbjct: 1   MALSAMNPGLALLFSLLLVGSCGCAANVVLIGTNVTLSFDSVEANFAPAIGSPGEPGVLY 60

Query: 61  LAKPLDACSTLENKVRLPVNSSSPFALIIRGGCSFEDKVRRAQVAGFKAAIVYDNEDSGS 120
           LAKPLDACSTLE+   LPVNSSSPF LIIRGGCSFEDKVRRAQ+AGFKAAI+YDNED G 
Sbjct: 61  LAKPLDACSTLESNTALPVNSSSPFVLIIRGGCSFEDKVRRAQMAGFKAAIIYDNEDGGG 120

Query: 121 LIAMAGSSAGIRIHAVFVTKSSGETLKKYAGLANVEIWIVPSFENSAWSIMAISFISLLA 180
           LIAMAGSSAGIRIHAVFVTKSSGETLKKYAGLANVEIWIVPSFENSAWSIMAISFISLLA
Sbjct: 121 LIAMAGSSAGIRIHAVFVTKSSGETLKKYAGLANVEIWIVPSFENSAWSIMAISFISLLA 180

Query: 181 MSAVLATCFFVRRHRIRRERPRSSHSQEFRGMSRRVVKAMPSLIFTTALEDNCTSVTCAI 240
           MSAVLATCFFVRRHRIRR RPRSSH+QEFRGMSRRVVKAMPSLIFTTALEDNCTSVTCAI
Sbjct: 181 MSAVLATCFFVRRHRIRRGRPRSSHAQEFRGMSRRVVKAMPSLIFTTALEDNCTSVTCAI 240

Query: 241 CLEDYTAGEKLRILPCRHSPGYMKLQHQGCPSFLAKEFHAFCVDSWLTAWRTFCPVCKRD 300
           CLEDYTAGEKLRILPCRH                  +FHA CVDSWLTAWRTFCPVCKRD
Sbjct: 241 CLEDYTAGEKLRILPCRH------------------KFHALCVDSWLTAWRTFCPVCKRD 300

Query: 301 ARTSTGDPPPSESTPLLSSTPNSVASSSILSSVRSSLPPSSAIQISPSSRSPSVSRNHSL 360
           ARTS GDPPPSESTPLLSSTP+SVASSSILSSVRSSLPPSSAIQISPSSRSPSVSRNHSL
Sbjct: 301 ARTSNGDPPPSESTPLLSSTPSSVASSSILSSVRSSLPPSSAIQISPSSRSPSVSRNHSL 360

Query: 361 SSTPYHQQSLRSSYHQSPSLSVSRSSVELRNASSQRSYASNLVSPYSSCYPSMSPLHSRY 420
           SSTPY QQSLRSSYHQ+ SLS+SRSSVELRNASSQRSYAS+LVSPYSSCYPSMSPLHSRY
Sbjct: 361 SSTPYLQQSLRSSYHQASSLSISRSSVELRNASSQRSYASHLVSPYSSCYPSMSPLHSRY 420

Query: 421 MIPLIPSPSNASPSIFGSSSHQQNPLHISGSAASFSPFASARSLPEC 468
           MIPLIPSPSNASPSI GSSSHQ NPLH SGSAASFSPFASA+SLPEC
Sbjct: 421 MIPLIPSPSNASPSIIGSSSHQHNPLHCSGSAASFSPFASAQSLPEC 449

BLAST of Spg039975 vs. NCBI nr
Match: XP_038885132.1 (receptor homology region, transmembrane domain- and RING domain-containing protein 2-like isoform X1 [Benincasa hispida])

HSP 1 Score: 773.9 bits (1997), Expect = 8.1e-220
Identity = 417/467 (89.29%), Postives = 430/467 (92.08%), Query Frame = 0

Query: 1   MALSAMNRGLALLFSLLLVGCCGSAANVVLIGTNVTLSFDSIEANFAPTIESSGEPGVLY 60
           MA SAMN GLALLFSL+LVGCCGSAANVVLIGTNVT SFDSIEANFAP+IESSGEPGVLY
Sbjct: 1   MAFSAMNPGLALLFSLILVGCCGSAANVVLIGTNVTRSFDSIEANFAPSIESSGEPGVLY 60

Query: 61  LAKPLDACSTLENKVRLPVNSSSPFALIIRGGCSFEDKVRRAQVAGFKAAIVYDNEDSGS 120
           LAKPLDACSTLENK  LPVNSSSPFALIIRGGCSFEDKVRRAQVAGFKAAI+YDNED G 
Sbjct: 61  LAKPLDACSTLENKTDLPVNSSSPFALIIRGGCSFEDKVRRAQVAGFKAAIIYDNED-GG 120

Query: 121 LIAMAGSSAGIRIHAVFVTKSSGETLKKYAGLANVEIWIVPSFENSAWSIMAISFISLLA 180
           LIAMAGSSAGIRIHAVFVTK+SGET+KKYAGL NVEIWIVPSFENS+WSIMAISFISLLA
Sbjct: 121 LIAMAGSSAGIRIHAVFVTKTSGETMKKYAGLPNVEIWIVPSFENSSWSIMAISFISLLA 180

Query: 181 MSAVLATCFFVRRHRIRRERPRSSHSQEFRGMSRRVVKAMPSLIFTTALEDNCTSVTCAI 240
           MSAVLATCFFVRRHRIRR RPRSSH+QEFRGMSRRVVKAMPSLIFTTALEDNCTS+TCAI
Sbjct: 181 MSAVLATCFFVRRHRIRRGRPRSSHAQEFRGMSRRVVKAMPSLIFTTALEDNCTSITCAI 240

Query: 241 CLEDYTAGEKLRILPCRHSPGYMKLQHQGCPSFLAKEFHAFCVDSWLTAWRTFCPVCKRD 300
           CLEDYTAGEKLRILPCRH                  +FHA CVDSWLTAWRTFCPVCKRD
Sbjct: 241 CLEDYTAGEKLRILPCRH------------------KFHALCVDSWLTAWRTFCPVCKRD 300

Query: 301 ARTSTGDPPPSESTPLLSSTPNSVASSSILSSVRSSLPPSSAIQISPSSRSPSVSRNHSL 360
           ARTS GDPPPSESTPLLSSTP+SVASSSILSSVRSSLPPSSAIQISPS RSPSVSRNHSL
Sbjct: 301 ARTSNGDPPPSESTPLLSSTPSSVASSSILSSVRSSLPPSSAIQISPSLRSPSVSRNHSL 360

Query: 361 SSTPYHQQSLRSSYHQSPSLSVSRSSVELRNASSQRSYASNLVSPYSSCYPSMSPLHSRY 420
           SSTPY QQSLRSSY QSP+LSVSRSSVELRNASSQRS  S+LVSPYSSCYPS+SPLHSRY
Sbjct: 361 SSTPYLQQSLRSSYRQSPTLSVSRSSVELRNASSQRSQTSHLVSPYSSCYPSISPLHSRY 420

Query: 421 MIPLIPSPSNASPSIFGSSSHQQNPLHISGSAASFSPFASARSLPEC 468
           MIPLIPSPSNASPSI GSSSHQ NPLH SGSAASFSPFASARSLPEC
Sbjct: 421 MIPLIPSPSNASPSIIGSSSHQHNPLHCSGSAASFSPFASARSLPEC 448

BLAST of Spg039975 vs. NCBI nr
Match: XP_023550607.1 (receptor homology region, transmembrane domain- and RING domain-containing protein 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 773.5 bits (1996), Expect = 1.1e-219
Identity = 416/467 (89.08%), Postives = 427/467 (91.43%), Query Frame = 0

Query: 1   MALSAMNRGLALLFSLLLVGCCGSAANVVLIGTNVTLSFDSIEANFAPTIESSGEPGVLY 60
           MALSAMN GLALLFSLLLVG CG AANVVLIGTNVTLSFDS+EANFAP I S GEPGVLY
Sbjct: 1   MALSAMNPGLALLFSLLLVGSCGCAANVVLIGTNVTLSFDSVEANFAPAIGSPGEPGVLY 60

Query: 61  LAKPLDACSTLENKVRLPVNSSSPFALIIRGGCSFEDKVRRAQVAGFKAAIVYDNEDSGS 120
           LAKPLDACSTLE+   LPVNSSSPF LIIRGGCSFEDKVRRAQ+AGFKAAI+YDNED G 
Sbjct: 61  LAKPLDACSTLESNTALPVNSSSPFVLIIRGGCSFEDKVRRAQMAGFKAAIIYDNEDGGG 120

Query: 121 LIAMAGSSAGIRIHAVFVTKSSGETLKKYAGLANVEIWIVPSFENSAWSIMAISFISLLA 180
           LIAMAGSSAGIRIHAVFVTKSSGETLKKYAGLANVEIWIVPSFENSAWSIMAISFISLLA
Sbjct: 121 LIAMAGSSAGIRIHAVFVTKSSGETLKKYAGLANVEIWIVPSFENSAWSIMAISFISLLA 180

Query: 181 MSAVLATCFFVRRHRIRRERPRSSHSQEFRGMSRRVVKAMPSLIFTTALEDNCTSVTCAI 240
           MSAVLATCFFVRRHRIRR RPRSSH+QEFRGMSRRVVKAMPSLIFTTALEDNCTSVTCAI
Sbjct: 181 MSAVLATCFFVRRHRIRRGRPRSSHAQEFRGMSRRVVKAMPSLIFTTALEDNCTSVTCAI 240

Query: 241 CLEDYTAGEKLRILPCRHSPGYMKLQHQGCPSFLAKEFHAFCVDSWLTAWRTFCPVCKRD 300
           CLEDYTAGEKLRILPCRH                  +FHA CVDSWLTAWRTFCPVCKRD
Sbjct: 241 CLEDYTAGEKLRILPCRH------------------KFHALCVDSWLTAWRTFCPVCKRD 300

Query: 301 ARTSTGDPPPSESTPLLSSTPNSVASSSILSSVRSSLPPSSAIQISPSSRSPSVSRNHSL 360
           ARTSTGDPPPSESTPLLSS P+SVASSSILSSVRSSLPPSSAIQISPSSRSPSVSRNH L
Sbjct: 301 ARTSTGDPPPSESTPLLSSAPSSVASSSILSSVRSSLPPSSAIQISPSSRSPSVSRNHYL 360

Query: 361 SSTPYHQQSLRSSYHQSPSLSVSRSSVELRNASSQRSYASNLVSPYSSCYPSMSPLHSRY 420
           SSTPY QQSLRSSYH + SLS+SRSSVELRNASSQRSYAS+LVSPYSSCYPSMSPLHSRY
Sbjct: 361 SSTPYLQQSLRSSYHHASSLSISRSSVELRNASSQRSYASHLVSPYSSCYPSMSPLHSRY 420

Query: 421 MIPLIPSPSNASPSIFGSSSHQQNPLHISGSAASFSPFASARSLPEC 468
           MIPLIPSPSNASPSI GSSSHQ NPLH SGSAASFSPFASA+SLPEC
Sbjct: 421 MIPLIPSPSNASPSIIGSSSHQHNPLHCSGSAASFSPFASAQSLPEC 449

BLAST of Spg039975 vs. NCBI nr
Match: XP_022993427.1 (receptor homology region, transmembrane domain- and RING domain-containing protein 2-like [Cucurbita maxima])

HSP 1 Score: 769.6 bits (1986), Expect = 1.5e-218
Identity = 414/467 (88.65%), Postives = 429/467 (91.86%), Query Frame = 0

Query: 1   MALSAMNRGLALLFSLLLVGCCGSAANVVLIGTNVTLSFDSIEANFAPTIESSGEPGVLY 60
           MALSAMN GLALLFSLLLVG CG AANVVLIGTNVTLSFDS+EANFAP I S GEPGVLY
Sbjct: 1   MALSAMNPGLALLFSLLLVGSCGCAANVVLIGTNVTLSFDSVEANFAPAIGSPGEPGVLY 60

Query: 61  LAKPLDACSTLENKVRLPVNSSSPFALIIRGGCSFEDKVRRAQVAGFKAAIVYDNEDSGS 120
           LAKPLDACSTLE+   LPVNSSSPFALIIRGGCSFEDKVRRAQ+AGFKAAI+YDNED G 
Sbjct: 61  LAKPLDACSTLESNTALPVNSSSPFALIIRGGCSFEDKVRRAQMAGFKAAIIYDNEDGGG 120

Query: 121 LIAMAGSSAGIRIHAVFVTKSSGETLKKYAGLANVEIWIVPSFENSAWSIMAISFISLLA 180
           LIAMAGSSAGIRIHAVFVTKSSGETLKKY+GLANVEIWIVPSFENSAWSIMAISFISLLA
Sbjct: 121 LIAMAGSSAGIRIHAVFVTKSSGETLKKYSGLANVEIWIVPSFENSAWSIMAISFISLLA 180

Query: 181 MSAVLATCFFVRRHRIRRERPRSSHSQEFRGMSRRVVKAMPSLIFTTALEDNCTSVTCAI 240
           MSAVLATCFFVRRHRIR+ RPRSSH+QEFRGMSRRVVKAMPSLIFTTALEDNCTSVTCAI
Sbjct: 181 MSAVLATCFFVRRHRIRQGRPRSSHAQEFRGMSRRVVKAMPSLIFTTALEDNCTSVTCAI 240

Query: 241 CLEDYTAGEKLRILPCRHSPGYMKLQHQGCPSFLAKEFHAFCVDSWLTAWRTFCPVCKRD 300
           CLEDYTAGEKLRILPCRH                  +FHA CVDSWLTAWRTFCPVCKRD
Sbjct: 241 CLEDYTAGEKLRILPCRH------------------KFHALCVDSWLTAWRTFCPVCKRD 300

Query: 301 ARTSTGDPPPSESTPLLSSTPNSVASSSILSSVRSSLPPSSAIQISPSSRSPSVSRNHSL 360
           ARTSTGDPPPSESTPLLSS P+SVASSSILSSVRSSLPPSSAIQISPSSRSPSVS+NHSL
Sbjct: 301 ARTSTGDPPPSESTPLLSSAPSSVASSSILSSVRSSLPPSSAIQISPSSRSPSVSQNHSL 360

Query: 361 SSTPYHQQSLRSSYHQSPSLSVSRSSVELRNASSQRSYASNLVSPYSSCYPSMSPLHSRY 420
           SSTPY +QSLRSS+HQS SLS+SRSSVELRNASSQRSYAS+LVSPYSSCYPSMSPLHSRY
Sbjct: 361 SSTPYLRQSLRSSHHQSSSLSISRSSVELRNASSQRSYASHLVSPYSSCYPSMSPLHSRY 420

Query: 421 MIPLIPSPSNASPSIFGSSSHQQNPLHISGSAASFSPFASARSLPEC 468
           MIPLIPS SNASPSI GSSSHQ NPLH SGSAASFSPFASA+SLPEC
Sbjct: 421 MIPLIPSSSNASPSIIGSSSHQHNPLHCSGSAASFSPFASAQSLPEC 449

BLAST of Spg039975 vs. ExPASy Swiss-Prot
Match: Q8VZ14 (Receptor homology region, transmembrane domain- and RING domain-containing protein 2 OS=Arabidopsis thaliana OX=3702 GN=RMR2 PE=2 SV=1)

HSP 1 Score: 514.2 bits (1323), Expect = 1.5e-144
Identity = 289/472 (61.23%), Postives = 354/472 (75.00%), Query Frame = 0

Query: 6   MNRGLALLFSLLLVGCCGSAANVVLIGTNVTLSFDSIEANFAPTIESSGEPGVLYLAKPL 65
           MNR L LL  +  V C  S + V+L+  N+TLSFD IEANFAP+++ +GE GV+Y+A+PL
Sbjct: 1   MNRALVLLLYVCTVSCLAS-SKVILMRNNITLSFDDIEANFAPSVKGTGEIGVVYVAEPL 60

Query: 66  DACSTLENKVRLPVNSSSPFALIIRGGCSFEDKVRRAQVAGFKAAIVYDNEDSGSLIAMA 125
           DAC  L NK     N +SPF LI+RGGCSFE+KVR+AQ AGFKAAI+YDNED G+LIAMA
Sbjct: 61  DACQNLMNKPEQSSNETSPFVLIVRGGCSFEEKVRKAQRAGFKAAIIYDNEDRGTLIAMA 120

Query: 126 GSSAGIRIHAVFVTKSSGETLKKYAGLANVEIWIVPSFENSAWSIMAISFISLLAMSAVL 185
           G+S GIRIHAVFVTK +GE LK+YAG  + ++W++PSFENSAWSIMA+SFISLLAMSAVL
Sbjct: 121 GNSGGIRIHAVFVTKETGEVLKEYAGFPDTKVWLIPSFENSAWSIMAVSFISLLAMSAVL 180

Query: 186 ATCFFVRRHRIRRERPRSSHSQEFRGMSRRVVKAMPSLIFTTALEDNCTSVTCAICLEDY 245
           ATCFFVRRHRIRR   RSS  +EF GMSRR+VKAMPSLIF++  EDN T+ TCAICLEDY
Sbjct: 181 ATCFFVRRHRIRRRTSRSSRVREFHGMSRRLVKAMPSLIFSSFHEDNTTAFTCAICLEDY 240

Query: 246 TAGEKLRILPCRHSPGYMKLQHQGCPSFLAKEFHAFCVDSWLTAWRTFCPVCKRDARTST 305
           T G+KLR+LPC H                  +FHA CVDSWLT+WRTFCPVCKRDARTST
Sbjct: 241 TVGDKLRLLPCCH------------------KFHAACVDSWLTSWRTFCPVCKRDARTST 300

Query: 306 GDPPPSESTPLLSSTPNSVASSSILSSVRSSLPPSSAIQISPS--SRSPSVSRNHSLSST 365
           G+PP SESTPLLSS  +S  SSS+ SSVR     SSA+ I PS  S   S+S + + +S+
Sbjct: 301 GEPPASESTPLLSSAASSFTSSSLHSSVR-----SSALLIGPSLGSLPTSISFSPAYASS 360

Query: 366 PYHQQSLRSSYH-QSPSLSVSRSSVELRN-------ASSQRSYASNLVSPYSSCYPSMSP 425
            Y +QS +SS + +SP +SVSRSSV+LR        + SQRSY S++ SP S  YP++SP
Sbjct: 361 SYIRQSFQSSSNRRSPPISVSRSSVDLRQQAASPSPSPSQRSYISHMASPQSLGYPTISP 420

Query: 426 LHSRYMIPLIPSPSNASPSIFGSSSHQQNPLHISGSAASFSPFASARSLPEC 468
            ++RYM P  PSPSNASP++ GSS++  NPL  S SA +FSP+ASA SLP+C
Sbjct: 421 FNTRYMSPYRPSPSNASPAMAGSSNYPLNPLRYSESAGTFSPYASANSLPDC 448

BLAST of Spg039975 vs. ExPASy Swiss-Prot
Match: Q10R93 (Receptor homology region, transmembrane domain- and RING domain-containing protein 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0167500 PE=2 SV=1)

HSP 1 Score: 416.4 bits (1069), Expect = 4.3e-115
Identity = 254/448 (56.70%), Postives = 309/448 (68.97%), Query Frame = 0

Query: 8   RGLALLFSLLLVGCCGS---AANVVLIGTNVTLSFDSIEANFAPTIESSGEPGVLYLAKP 67
           R + LL  L    C  +   AANVVL+GTN+TLSFD +EA+FAP ++ SG  GV+Y A+P
Sbjct: 5   RTMLLLICLCATFCLMTQLGAANVVLMGTNLTLSFDDVEASFAPGVKGSGFEGVVYTAEP 64

Query: 68  LDACSTLENKVRLPVNSSSPFALIIRGGCSFEDKVRRAQVAGFKAAIVYDNEDSGSLIAM 127
           LDACS L +K        SPFALIIRGGC+F++KV+ AQ AGFKAAIVYDNE+SG LI+M
Sbjct: 65  LDACSPLTSKAE--KGPPSPFALIIRGGCTFDEKVKNAQDAGFKAAIVYDNENSGVLISM 124

Query: 128 AGSSAGIRIHAVFVTKSSGETLKKYAGLANVEIWIVPSFENSAWSIMAISFISLLAMSAV 187
           AGSS GI I+AVF++K+SGE LKK++G  +VE+WI+P+FENSAWSIMAISFISLLAMSAV
Sbjct: 125 AGSSGGIHIYAVFISKASGEVLKKFSGHTDVEVWILPAFENSAWSIMAISFISLLAMSAV 184

Query: 188 LATCFFVRRHRIRRERPRSSHSQEFRGMSRRVVKAMPSLIFTTALEDNCTSVTCAICLED 247
           LATCFFVRRH IRR+RPR   ++EF GMS ++VKAMPSLIFT   EDNCTS  CAICLED
Sbjct: 185 LATCFFVRRHHIRRDRPRIPEAREFHGMSSQLVKAMPSLIFTKVQEDNCTSSMCAICLED 244

Query: 248 YTAGEKLRILPCRHSPGYMKLQHQGCPSFLAKEFHAFCVDSWLTAWRTFCPVCKRDARTS 307
           Y  GEKLR+LPCRH                  +FHA CVD WLT WRTFCPVCKRDA T 
Sbjct: 245 YNVGEKLRVLPCRH------------------KFHAACVDLWLTTWRTFCPVCKRDASTG 304

Query: 308 TGDPPPSESTPLLSSTPNSVASSSILSSVRSSLPPSSAIQISPSSRSPSVSRNHSLSSTP 367
             DPP SE+TPLLSS   +V   S  SS RSS+  S    IS    S S+SR ++ S TP
Sbjct: 305 IPDPPASETTPLLSS---AVRLPSQSSSFRSSVAASPPRPISRRPSSQSISRIYAASGTP 364

Query: 368 YHQQSLRSSYHQSPSLSVSRSSVELRNASSQRSYASNLVSPYSSCYPSMS-PLHSRYMIP 427
                +R S+  S ++S+SRS+V+L N SS R  AS+L S +S     +S P++ RY  P
Sbjct: 365 NSPNPIR-SFTNSTAMSISRSNVDLSNMSS-RPRASHLASAHSLVGSHLSPPINIRYASP 424

Query: 428 LIPSPSNASPSIFGSSSHQQNPLHISGS 452
            +     ASPS   SSS+  N  + S S
Sbjct: 425 HMSHSGYASPSPHVSSSYVSNSGYGSSS 427

BLAST of Spg039975 vs. ExPASy Swiss-Prot
Match: Q69U49 (Receptor homology region, transmembrane domain- and RING domain-containing protein 2 OS=Oryza sativa subsp. japonica OX=39947 GN=RMR1 PE=2 SV=2)

HSP 1 Score: 396.0 bits (1016), Expect = 6.0e-109
Identity = 241/449 (53.67%), Postives = 300/449 (66.82%), Query Frame = 0

Query: 9   GLALLF-SLLLVGCCGSAANVVLIGTNVTLSFDSIEANFAPTIESSGEPGVLYLAKPLDA 68
           G  LLF +++ +     A NVVLI  N TLSFD +EA F P ++ SG  G +Y  +PLDA
Sbjct: 8   GFPLLFCAVICLMAQQGACNVVLIANNTTLSFDDVEATFTPEVKDSGVNGAIYAVEPLDA 67

Query: 69  CSTLENK-VRLPVNSSSPFALIIRGGCSFEDKVRRAQVAGFKAAIVYDNEDSGSLIAMAG 128
           CS L  K    PV   SPFAL+IRGGC F+DKVR AQ AGFKA IVYD+EDSG L++MAG
Sbjct: 68  CSPLRKKAANGPV---SPFALVIRGGCQFDDKVRNAQNAGFKAVIVYDDEDSGVLVSMAG 127

Query: 129 SSAGIRIHAVFVTKSSGETLKKYAGLANVEIWIVPSFENSAWSIMAISFISLLAMSAVLA 188
           SS+GI I+AVF++K+SGE LKKY+G ++VE+WI+P +ENSAWSIMAISF SLLAM+AVLA
Sbjct: 128 SSSGIYIYAVFLSKASGEVLKKYSGQSDVEVWILPVYENSAWSIMAISFTSLLAMAAVLA 187

Query: 189 TCFFVRRHRIRRERPRSSHSQEFRGMSRRVVKAMPSLIFTTALEDNCTSVTCAICLEDYT 248
           TCFFVRRH+IRR+R R   ++EF GMS ++VKAMPSLIFT   EDN TS +CAICLEDY+
Sbjct: 188 TCFFVRRHQIRRDRGRIPVTREFHGMSSQLVKAMPSLIFTKVQEDNSTSSSCAICLEDYS 247

Query: 249 AGEKLRILPCRHSPGYMKLQHQGCPSFLAKEFHAFCVDSWLTAWRTFCPVCKRDARTSTG 308
            GEKLR+LPCRH                  +FHA CVD WLT+W+TFCPVCKRDA   T 
Sbjct: 248 FGEKLRVLPCRH------------------KFHATCVDMWLTSWKTFCPVCKRDASAGTS 307

Query: 309 DPPPSESTPLLSSTPNSVASSSILSSVRSSLPPSSAIQISPSSRSPSVSRNHSLSSTPYH 368
            PP SESTPLLSS  +  A S+ LSS RS++  S    I     S S SR +S+SS P  
Sbjct: 308 KPPASESTPLLSSVIHLSAESTALSSFRSTVAVSPPRPIRRHPSSQSTSRAYSISSAP-R 367

Query: 369 QQSLRSSYHQSPSLSVSRSSVELRNASSQRSYASNLVSPYSSCYPSMS-PLHSRYMIPLI 428
             +L+  Y  SP +S SRS+V+L N SSQ S+  +  S +S     +S P++ RY IP +
Sbjct: 368 NYNLQRYYTNSPYISTSRSNVDLANMSSQWSHTPHQASMHSLRSGHLSLPINIRYTIPHV 427

Query: 429 PSPSNASPSI---FGSSSHQQNPLHISGS 452
                 S S+     S SH  +P +   S
Sbjct: 428 SRSDYGSASLGLSHDSCSHHGSPSYYHSS 434

BLAST of Spg039975 vs. ExPASy Swiss-Prot
Match: Q0WPW5 (Receptor homology region, transmembrane domain- and RING domain-containing protein 4 OS=Arabidopsis thaliana OX=3702 GN=RMR4 PE=2 SV=1)

HSP 1 Score: 356.3 bits (913), Expect = 5.3e-97
Identity = 228/475 (48.00%), Postives = 295/475 (62.11%), Query Frame = 0

Query: 6   MNRGLALLFSLLLVGCCGSAANVVLIGTNVTLSFDSIEANFAPTIESSGEPGVLYLAKPL 65
           M R   ++ SLLL+    S A V+LIG + +LSFD +EA F P I+ S + GVLY+A+PL
Sbjct: 1   MIRSSIVILSLLLISHLVS-AKVLLIGNSTSLSFDDVEATFTPMIKRSDQGGVLYVAEPL 60

Query: 66  DACSTLENKVRLP--VNSSSPFALIIRGGCSFEDKVRRAQVAGFKAAIVYDNEDSGSLIA 125
           DACS L N V +      S P+ LIIRGGCSFEDK+R AQ AG+KAAIVYD ED G L++
Sbjct: 61  DACSDLVNTVNVKNGTTVSPPYVLIIRGGCSFEDKIRNAQKAGYKAAIVYDYEDFGFLVS 120

Query: 126 MAGSSAGIRIHAVFVTKSSGETLKKYAGLANVEIWIVPSFENSAWSIMAISFISLLAMSA 185
           MAG+ +G+ I+  FV+K++GE LK+YAG  + E+W++PSFE SAWSIMAISFISLLAMSA
Sbjct: 121 MAGNPSGVLIYGTFVSKATGEVLKEYAGRTDFEVWLMPSFETSAWSIMAISFISLLAMSA 180

Query: 186 VLATCFFVRRHRIRRERPRSSHSQEFRGMSRRVVKAMPSLIFTTALEDNCTSVTCAICLE 245
           VLATCFFVRRHR+RR R  + +  +F  M + ++  MP+ IF    ++  TS+ C ICLE
Sbjct: 181 VLATCFFVRRHRVRRRRILALNGNDFHRMPKSMIIRMPTTIFNGICDEATTSILCCICLE 240

Query: 246 DYTAGEKLRILPCRHSPGYMKLQHQGCPSFLAKEFHAFCVDSWLTAWRTFCPVCKRDART 305
           +Y  G+KLRILPC H                  +FH  CVD WL   ++FCPVCKRDAR+
Sbjct: 241 NYEKGDKLRILPCHH------------------KFHVACVDLWLGQRKSFCPVCKRDARS 300

Query: 306 STGDPPPSESTPLLSSTPNSVASSSILSSVRSSLPPSS------AIQISPSSRSPSVS-- 365
            + D PPSE TP LS TP+   +SS L S  S+ P  S      +I++ PS  S S+   
Sbjct: 301 ISTDKPPSEHTPFLSRTPSMTPTSSFLLSSSSTTPLQSSHELPISIRVDPSLPSTSMQPH 360

Query: 366 -----RNHSLSSTPYHQQSLRSSYHQSPSLSVSRSSVELRNASSQRSYASNLVSPYSSCY 425
                 +HS S T +   S R     S  + VSRSS +LRNA SQRSY     SP+    
Sbjct: 361 TVPMYLSHSRSHTSFQNGSNRF----SRPIPVSRSSADLRNAVSQRSYN----SPHQVSL 420

Query: 426 PSMSPLHSRYMIPLIPSPSNASPSIFGSSSHQQNPLHISGSAASFSPFASARSLP 466
           P    LHSRY   L P  ++ S  +   +S +++ LH + S  SF  FASA SLP
Sbjct: 421 PRF--LHSRYTHILGPGNASRSQVVGLLTSQREHSLHQNDSRRSFIHFASASSLP 446

BLAST of Spg039975 vs. ExPASy Swiss-Prot
Match: F4I2Y3 (Receptor homology region, transmembrane domain- and RING domain-containing protein 3 OS=Arabidopsis thaliana OX=3702 GN=RMR3 PE=3 SV=1)

HSP 1 Score: 307.8 bits (787), Expect = 2.2e-82
Identity = 216/466 (46.35%), Postives = 283/466 (60.73%), Query Frame = 0

Query: 6   MNRGLALLFSLLL--VGCCGSAANVVLIGTNVTLSFDSIEANFAPTIESSGEPGVLYLAK 65
           MN  + L+ +LLL  V     A  V+L+ +N+T SF  +EA+F+P++ ++ E GV+Y+A+
Sbjct: 1   MNLVVLLILTLLLFIVSYVVDAGQVILVDSNITRSFVDMEADFSPSV-TTVETGVVYVAE 60

Query: 66  PLDACSTLENKVRLPVNSSSPFALIIRGGCSFEDKVRRAQVAGFKAAIVYDNEDSGSLIA 125
           PL+AC  L NK       +SP  LIIRGGCSFE KVR AQ +GFKAAIVYDN D   L A
Sbjct: 61  PLNACRNLRNKPEQSPYGTSPLVLIIRGGCSFEYKVRNAQRSGFKAAIVYDNVDRNFLSA 120

Query: 126 MAGSSAGIRIHAVFVTKSSGETLKKYAGLANVEIWIV-PSFENSAWSIMA-ISFISLLAM 185
           M G S GI+I AVFV K +GE LKKYAG   +E+ +V P+ E+S WS+ A I+ I  LA+
Sbjct: 121 MGGDSDGIKIQAVFVMKRAGEMLKKYAGSEEMEVMLVPPNTEDSVWSLYASIALILSLAI 180

Query: 186 SAVLATCFFVRRHRIRRERPRSSHSQEFRGMSRRVVKAMPSLIFTTALEDNCTSVTCAIC 245
             V+ TC F  R+       R+S SQ F GM RR VKAMPS+ FT A  DN T  +CAIC
Sbjct: 181 FCVMVTCVFFYRY---CSTIRNSTSQ-FNGMCRRTVKAMPSVTFTCAKIDNTTGFSCAIC 240

Query: 246 LEDYTAGEKLRILPCRHSPGYMKLQHQGCPSFLAKEFHAFCVDSWLTAWRTFCPVCKRDA 305
           LEDY  G+KLR+LPC H                  +FH  CVDSWL +WRTFCPVCKRDA
Sbjct: 241 LEDYIVGDKLRVLPCSH------------------KFHVACVDSWLISWRTFCPVCKRDA 300

Query: 306 RTSTGDPPPSESTPLLSSTPNSVASSSILSSVRSSLPPSSAIQISPSSRSPSVSRNHSLS 365
           RT+  +P  +ESTP LSS   S+A+SS++     S P  S++  SP+  S S        
Sbjct: 301 RTTADEPLATESTPFLSS---SIATSSLVCI--DSPPLGSSVSFSPAHVSSS-------- 360

Query: 366 STPYHQQSLRSSYHQSPSLSVS-RSSVELRNASSQRSYASNLVSPYSSCYPSMSPLHSRY 425
              +  Q +RSS      +S + R       +SSQRS+ S + S +S  Y +MSPL++  
Sbjct: 361 ---FIHQFVRSSPMNGSRISENLRRQASPLQSSSQRSHLS-MKSSHSLGYSTMSPLNAMG 420

Query: 426 MIPLIPSPSNASPSIFGSSSHQQNPLHISGSAASFSPFASARSLPE 467
           M P  P PSNASP +F S++H    L  + +A +FS FASA SLP+
Sbjct: 421 MSPYRPYPSNASPGLFSSTNH----LLSNYTANTFSHFASAHSLPD 422

BLAST of Spg039975 vs. ExPASy TrEMBL
Match: A0A6J1FL26 (receptor homology region, transmembrane domain- and RING domain-containing protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC111445201 PE=4 SV=1)

HSP 1 Score: 777.7 bits (2007), Expect = 2.7e-221
Identity = 418/467 (89.51%), Postives = 429/467 (91.86%), Query Frame = 0

Query: 1   MALSAMNRGLALLFSLLLVGCCGSAANVVLIGTNVTLSFDSIEANFAPTIESSGEPGVLY 60
           MALSAMN GLALLFSLLLVG CG AANVVLIGTNVTLSFDS+EANFAP I S GEPGVLY
Sbjct: 1   MALSAMNPGLALLFSLLLVGSCGCAANVVLIGTNVTLSFDSVEANFAPAIGSPGEPGVLY 60

Query: 61  LAKPLDACSTLENKVRLPVNSSSPFALIIRGGCSFEDKVRRAQVAGFKAAIVYDNEDSGS 120
           LAKPLDACSTLE+   LPVNSSSPF LIIRGGCSFEDKVRRAQ+AGFKAAI+YDNED G 
Sbjct: 61  LAKPLDACSTLESNTALPVNSSSPFVLIIRGGCSFEDKVRRAQMAGFKAAIIYDNEDGGG 120

Query: 121 LIAMAGSSAGIRIHAVFVTKSSGETLKKYAGLANVEIWIVPSFENSAWSIMAISFISLLA 180
           LIAMAGSSAGIRIHAVFVTKSSGETLKKYAGLANVEIWIVPSFENSAWSIMAISFISLLA
Sbjct: 121 LIAMAGSSAGIRIHAVFVTKSSGETLKKYAGLANVEIWIVPSFENSAWSIMAISFISLLA 180

Query: 181 MSAVLATCFFVRRHRIRRERPRSSHSQEFRGMSRRVVKAMPSLIFTTALEDNCTSVTCAI 240
           MSAVLATCFFVRRHRIRR RPRSSH+QEFRGMSRRVVKAMPSLIFTTALEDNCTSVTCAI
Sbjct: 181 MSAVLATCFFVRRHRIRRGRPRSSHAQEFRGMSRRVVKAMPSLIFTTALEDNCTSVTCAI 240

Query: 241 CLEDYTAGEKLRILPCRHSPGYMKLQHQGCPSFLAKEFHAFCVDSWLTAWRTFCPVCKRD 300
           CLEDYTAGEKLRILPCRH                  +FHA CVDSWLTAWRTFCPVCKRD
Sbjct: 241 CLEDYTAGEKLRILPCRH------------------KFHALCVDSWLTAWRTFCPVCKRD 300

Query: 301 ARTSTGDPPPSESTPLLSSTPNSVASSSILSSVRSSLPPSSAIQISPSSRSPSVSRNHSL 360
           ARTSTGDPPPSESTPLLSS P+SVASSSILSSVRSSLPPSSAIQISPSSRSPSVSRNHSL
Sbjct: 301 ARTSTGDPPPSESTPLLSSAPSSVASSSILSSVRSSLPPSSAIQISPSSRSPSVSRNHSL 360

Query: 361 SSTPYHQQSLRSSYHQSPSLSVSRSSVELRNASSQRSYASNLVSPYSSCYPSMSPLHSRY 420
           SSTPY QQSLRSSYHQ+ SLS+SRSSVELRNASSQRSYAS+LVSPYSSCYPSMSPLHSRY
Sbjct: 361 SSTPYLQQSLRSSYHQASSLSISRSSVELRNASSQRSYASHLVSPYSSCYPSMSPLHSRY 420

Query: 421 MIPLIPSPSNASPSIFGSSSHQQNPLHISGSAASFSPFASARSLPEC 468
           MIPLIPSPSNASPSI GSSSHQ NPLH SGSAASFSPFASA+SLPEC
Sbjct: 421 MIPLIPSPSNASPSIIGSSSHQHNPLHCSGSAASFSPFASAQSLPEC 449

BLAST of Spg039975 vs. ExPASy TrEMBL
Match: A0A6J1JSS3 (receptor homology region, transmembrane domain- and RING domain-containing protein 2-like OS=Cucurbita maxima OX=3661 GN=LOC111489445 PE=4 SV=1)

HSP 1 Score: 769.6 bits (1986), Expect = 7.4e-219
Identity = 414/467 (88.65%), Postives = 429/467 (91.86%), Query Frame = 0

Query: 1   MALSAMNRGLALLFSLLLVGCCGSAANVVLIGTNVTLSFDSIEANFAPTIESSGEPGVLY 60
           MALSAMN GLALLFSLLLVG CG AANVVLIGTNVTLSFDS+EANFAP I S GEPGVLY
Sbjct: 1   MALSAMNPGLALLFSLLLVGSCGCAANVVLIGTNVTLSFDSVEANFAPAIGSPGEPGVLY 60

Query: 61  LAKPLDACSTLENKVRLPVNSSSPFALIIRGGCSFEDKVRRAQVAGFKAAIVYDNEDSGS 120
           LAKPLDACSTLE+   LPVNSSSPFALIIRGGCSFEDKVRRAQ+AGFKAAI+YDNED G 
Sbjct: 61  LAKPLDACSTLESNTALPVNSSSPFALIIRGGCSFEDKVRRAQMAGFKAAIIYDNEDGGG 120

Query: 121 LIAMAGSSAGIRIHAVFVTKSSGETLKKYAGLANVEIWIVPSFENSAWSIMAISFISLLA 180
           LIAMAGSSAGIRIHAVFVTKSSGETLKKY+GLANVEIWIVPSFENSAWSIMAISFISLLA
Sbjct: 121 LIAMAGSSAGIRIHAVFVTKSSGETLKKYSGLANVEIWIVPSFENSAWSIMAISFISLLA 180

Query: 181 MSAVLATCFFVRRHRIRRERPRSSHSQEFRGMSRRVVKAMPSLIFTTALEDNCTSVTCAI 240
           MSAVLATCFFVRRHRIR+ RPRSSH+QEFRGMSRRVVKAMPSLIFTTALEDNCTSVTCAI
Sbjct: 181 MSAVLATCFFVRRHRIRQGRPRSSHAQEFRGMSRRVVKAMPSLIFTTALEDNCTSVTCAI 240

Query: 241 CLEDYTAGEKLRILPCRHSPGYMKLQHQGCPSFLAKEFHAFCVDSWLTAWRTFCPVCKRD 300
           CLEDYTAGEKLRILPCRH                  +FHA CVDSWLTAWRTFCPVCKRD
Sbjct: 241 CLEDYTAGEKLRILPCRH------------------KFHALCVDSWLTAWRTFCPVCKRD 300

Query: 301 ARTSTGDPPPSESTPLLSSTPNSVASSSILSSVRSSLPPSSAIQISPSSRSPSVSRNHSL 360
           ARTSTGDPPPSESTPLLSS P+SVASSSILSSVRSSLPPSSAIQISPSSRSPSVS+NHSL
Sbjct: 301 ARTSTGDPPPSESTPLLSSAPSSVASSSILSSVRSSLPPSSAIQISPSSRSPSVSQNHSL 360

Query: 361 SSTPYHQQSLRSSYHQSPSLSVSRSSVELRNASSQRSYASNLVSPYSSCYPSMSPLHSRY 420
           SSTPY +QSLRSS+HQS SLS+SRSSVELRNASSQRSYAS+LVSPYSSCYPSMSPLHSRY
Sbjct: 361 SSTPYLRQSLRSSHHQSSSLSISRSSVELRNASSQRSYASHLVSPYSSCYPSMSPLHSRY 420

Query: 421 MIPLIPSPSNASPSIFGSSSHQQNPLHISGSAASFSPFASARSLPEC 468
           MIPLIPS SNASPSI GSSSHQ NPLH SGSAASFSPFASA+SLPEC
Sbjct: 421 MIPLIPSSSNASPSIIGSSSHQHNPLHCSGSAASFSPFASAQSLPEC 449

BLAST of Spg039975 vs. ExPASy TrEMBL
Match: A0A6J1DE26 (receptor homology region, transmembrane domain- and RING domain-containing protein 2-like OS=Momordica charantia OX=3673 GN=LOC111020208 PE=4 SV=1)

HSP 1 Score: 768.1 bits (1982), Expect = 2.2e-218
Identity = 412/467 (88.22%), Postives = 427/467 (91.43%), Query Frame = 0

Query: 1   MALSAMNRGLALLFSLLLVGCCGSAANVVLIGTNVTLSFDSIEANFAPTIESSGEPGVLY 60
           MA SAMN GLALLFSLL VGCC SAANVVLIGTNVTLSFDSIEANFAP+I+SSGEPGVLY
Sbjct: 1   MAWSAMNPGLALLFSLLFVGCCRSAANVVLIGTNVTLSFDSIEANFAPSIKSSGEPGVLY 60

Query: 61  LAKPLDACSTLENKVRLPVNSSSPFALIIRGGCSFEDKVRRAQVAGFKAAIVYDNEDSGS 120
           +AKPLDACS L NKV L VNSSSPFAL+IRGGCSFEDKVRRAQVAGFKAAI+YDNED G+
Sbjct: 61  VAKPLDACSALTNKVELSVNSSSPFALMIRGGCSFEDKVRRAQVAGFKAAIIYDNEDGGA 120

Query: 121 LIAMAGSSAGIRIHAVFVTKSSGETLKKYAGLANVEIWIVPSFENSAWSIMAISFISLLA 180
           LIAMAGSSAGIRIHAVFVTK SGETLKKYAG+AN EIWIVPSFENSAWSIMAISFISLLA
Sbjct: 121 LIAMAGSSAGIRIHAVFVTKESGETLKKYAGMANTEIWIVPSFENSAWSIMAISFISLLA 180

Query: 181 MSAVLATCFFVRRHRIRRERPRSSHSQEFRGMSRRVVKAMPSLIFTTALEDNCTSVTCAI 240
           MSAVLATCFFVRRHRIRRERPRSSH QEFRGMSRRVVKAMPSLIFTTALEDNCTS+TCAI
Sbjct: 181 MSAVLATCFFVRRHRIRRERPRSSHLQEFRGMSRRVVKAMPSLIFTTALEDNCTSITCAI 240

Query: 241 CLEDYTAGEKLRILPCRHSPGYMKLQHQGCPSFLAKEFHAFCVDSWLTAWRTFCPVCKRD 300
           CLEDYTAGEKLRILPCRH                  +FHA CVDSWLTAWRTFCPVCKRD
Sbjct: 241 CLEDYTAGEKLRILPCRH------------------KFHAICVDSWLTAWRTFCPVCKRD 300

Query: 301 ARTSTGDPPPSESTPLLSSTPNSVASSSILSSVRSSLPPSSAIQISPSSRSPSVSRNHSL 360
           ARTST +PPP+ESTPLLSS PNSVASSSILSSVRSSLPPSSAIQISP+SRSPSVSRNHSL
Sbjct: 301 ARTSTSNPPPAESTPLLSSAPNSVASSSILSSVRSSLPPSSAIQISPASRSPSVSRNHSL 360

Query: 361 SSTPYHQQSLRSSYHQSPSLSVSRSSVELRNASSQRSYASNLVSPYSSCYPSMSPLHSRY 420
           SSTPY QQSLRSSY QSPSLSVSRSSVEL+NASSQRSYAS+LVSPYSSCYPS+SPLHSRY
Sbjct: 361 SSTPYLQQSLRSSYRQSPSLSVSRSSVELQNASSQRSYASHLVSPYSSCYPSISPLHSRY 420

Query: 421 MIPLIPSPSNASPSIFGSSSHQQNPLHISGSAASFSPFASARSLPEC 468
           MIPLIPSPSNASPS  GSSSHQQNPLH S SA SFSPFASARSLPEC
Sbjct: 421 MIPLIPSPSNASPSFMGSSSHQQNPLHCSESATSFSPFASARSLPEC 449

BLAST of Spg039975 vs. ExPASy TrEMBL
Match: A0A1S3B2Y0 (receptor homology region, transmembrane domain- and RING domain-containing protein 2-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103485424 PE=4 SV=1)

HSP 1 Score: 746.5 bits (1926), Expect = 6.7e-212
Identity = 404/467 (86.51%), Postives = 422/467 (90.36%), Query Frame = 0

Query: 1   MALSAMNRGLALLFSLLLVGCCGSAANVVLIGTNVTLSFDSIEANFAPTIESSGEPGVLY 60
           + L+ MN  LALLFSLLLV   GSAANVVLIG N+TLSFDSIEANFAP+IESSGEPGVLY
Sbjct: 4   LLLTPMNPRLALLFSLLLVLSSGSAANVVLIGANITLSFDSIEANFAPSIESSGEPGVLY 63

Query: 61  LAKPLDACSTLENKVRLPVNSSSPFALIIRGGCSFEDKVRRAQVAGFKAAIVYDNEDSGS 120
           LAKPLDACSTLENKV +PVNSSSPFALI+RGGCSFEDKVRRAQVAGFKAAI+YDNED G 
Sbjct: 64  LAKPLDACSTLENKVVVPVNSSSPFALIVRGGCSFEDKVRRAQVAGFKAAIIYDNED-GG 123

Query: 121 LIAMAGSSAGIRIHAVFVTKSSGETLKKYAGLANVEIWIVPSFENSAWSIMAISFISLLA 180
           LIAMAGSSAGIRIHAVFVTK+SGETLKKYAGLANVEIWI+PSFENSAWSIMAISFISLLA
Sbjct: 124 LIAMAGSSAGIRIHAVFVTKTSGETLKKYAGLANVEIWIIPSFENSAWSIMAISFISLLA 183

Query: 181 MSAVLATCFFVRRHRIRRERPRSSHSQEFRGMSRRVVKAMPSLIFTTALEDNCTSVTCAI 240
           MSAVLATCFFVRRHRIRR RPRS +SQEFRGMSRRVVKAMPSLIFTTALEDNCTS+TCAI
Sbjct: 184 MSAVLATCFFVRRHRIRRGRPRSFYSQEFRGMSRRVVKAMPSLIFTTALEDNCTSITCAI 243

Query: 241 CLEDYTAGEKLRILPCRHSPGYMKLQHQGCPSFLAKEFHAFCVDSWLTAWRTFCPVCKRD 300
           CLEDY+ GEKLRILPCRH                  +FHA CVDSWLTAWRTFCPVCKRD
Sbjct: 244 CLEDYSPGEKLRILPCRH------------------KFHALCVDSWLTAWRTFCPVCKRD 303

Query: 301 ARTSTGDPPPSESTPLLSSTPNSVASSSILSSVRSSLPPSSAIQISPSSRSPSVSRNHSL 360
           ARTSTGDPPPSESTPLLSS P+SVASSSILSS RSSLPPSSAIQISPS RSPSVSRNHSL
Sbjct: 304 ARTSTGDPPPSESTPLLSSAPSSVASSSILSSSRSSLPPSSAIQISPSLRSPSVSRNHSL 363

Query: 361 SSTPYHQQSLRSSYHQSPSLSVSRSSVELRNASSQRSYASNLVSPYSSCYPSMSPLHSRY 420
           S TPY  QSLRSSYHQSPSLSVSRSSVELRNASSQRSY S+L+SPYSSCYPS+SPLHSRY
Sbjct: 364 SGTPYLHQSLRSSYHQSPSLSVSRSSVELRNASSQRSYTSHLISPYSSCYPSVSPLHSRY 423

Query: 421 MIPLIPSPSNASPSIFGSSSHQQNPLHISGSAASFSPFASARSLPEC 468
           +IP IPSPSNASPSI GSSSHQ NPLH SGSAAS SPFASARSLPEC
Sbjct: 424 LIPHIPSPSNASPSIIGSSSHQHNPLHCSGSAASLSPFASARSLPEC 451

BLAST of Spg039975 vs. ExPASy TrEMBL
Match: A0A1S3B2Z2 (receptor homology region, transmembrane domain- and RING domain-containing protein 2-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103485424 PE=4 SV=1)

HSP 1 Score: 741.5 bits (1913), Expect = 2.2e-210
Identity = 401/469 (85.50%), Postives = 421/469 (89.77%), Query Frame = 0

Query: 1   MALSAMNRGLALLFSLLLVGCCGSAANVVLIGTNVTLSFDSIEANFAPTIESSGEPGVLY 60
           + L+ MN  LALLFSLLLV   GSAANVVLIG N+TLSFDSIEANFAP+IESSGEPGVLY
Sbjct: 4   LLLTPMNPRLALLFSLLLVLSSGSAANVVLIGANITLSFDSIEANFAPSIESSGEPGVLY 63

Query: 61  LAKPLDACSTLENKVRLPVNSSSPFALIIRGGCSFEDKVRRAQVAGFKAAIVYDNEDSGS 120
           LAKPLDACSTLENKV +PVNSSSPFALI+RGGCSFEDKVRRAQVAGFKAAI+YDNED G 
Sbjct: 64  LAKPLDACSTLENKVVVPVNSSSPFALIVRGGCSFEDKVRRAQVAGFKAAIIYDNEDGGL 123

Query: 121 L--IAMAGSSAGIRIHAVFVTKSSGETLKKYAGLANVEIWIVPSFENSAWSIMAISFISL 180
           +   A+AGSSAGIRIHAVFVTK+SGETLKKYAGLANVEIWI+PSFENSAWSIMAISFISL
Sbjct: 124 IAKFAVAGSSAGIRIHAVFVTKTSGETLKKYAGLANVEIWIIPSFENSAWSIMAISFISL 183

Query: 181 LAMSAVLATCFFVRRHRIRRERPRSSHSQEFRGMSRRVVKAMPSLIFTTALEDNCTSVTC 240
           LAMSAVLATCFFVRRHRIRR RPRS +SQEFRGMSRRVVKAMPSLIFTTALEDNCTS+TC
Sbjct: 184 LAMSAVLATCFFVRRHRIRRGRPRSFYSQEFRGMSRRVVKAMPSLIFTTALEDNCTSITC 243

Query: 241 AICLEDYTAGEKLRILPCRHSPGYMKLQHQGCPSFLAKEFHAFCVDSWLTAWRTFCPVCK 300
           AICLEDY+ GEKLRILPCRH                  +FHA CVDSWLTAWRTFCPVCK
Sbjct: 244 AICLEDYSPGEKLRILPCRH------------------KFHALCVDSWLTAWRTFCPVCK 303

Query: 301 RDARTSTGDPPPSESTPLLSSTPNSVASSSILSSVRSSLPPSSAIQISPSSRSPSVSRNH 360
           RDARTSTGDPPPSESTPLLSS P+SVASSSILSS RSSLPPSSAIQISPS RSPSVSRNH
Sbjct: 304 RDARTSTGDPPPSESTPLLSSAPSSVASSSILSSSRSSLPPSSAIQISPSLRSPSVSRNH 363

Query: 361 SLSSTPYHQQSLRSSYHQSPSLSVSRSSVELRNASSQRSYASNLVSPYSSCYPSMSPLHS 420
           SLS TPY  QSLRSSYHQSPSLSVSRSSVELRNASSQRSY S+L+SPYSSCYPS+SPLHS
Sbjct: 364 SLSGTPYLHQSLRSSYHQSPSLSVSRSSVELRNASSQRSYTSHLISPYSSCYPSVSPLHS 423

Query: 421 RYMIPLIPSPSNASPSIFGSSSHQQNPLHISGSAASFSPFASARSLPEC 468
           RY+IP IPSPSNASPSI GSSSHQ NPLH SGSAAS SPFASARSLPEC
Sbjct: 424 RYLIPHIPSPSNASPSIIGSSSHQHNPLHCSGSAASLSPFASARSLPEC 454

BLAST of Spg039975 vs. TAIR 10
Match: AT1G71980.1 (Protease-associated (PA) RING/U-box zinc finger family protein )

HSP 1 Score: 514.2 bits (1323), Expect = 1.1e-145
Identity = 289/472 (61.23%), Postives = 354/472 (75.00%), Query Frame = 0

Query: 6   MNRGLALLFSLLLVGCCGSAANVVLIGTNVTLSFDSIEANFAPTIESSGEPGVLYLAKPL 65
           MNR L LL  +  V C  S + V+L+  N+TLSFD IEANFAP+++ +GE GV+Y+A+PL
Sbjct: 1   MNRALVLLLYVCTVSCLAS-SKVILMRNNITLSFDDIEANFAPSVKGTGEIGVVYVAEPL 60

Query: 66  DACSTLENKVRLPVNSSSPFALIIRGGCSFEDKVRRAQVAGFKAAIVYDNEDSGSLIAMA 125
           DAC  L NK     N +SPF LI+RGGCSFE+KVR+AQ AGFKAAI+YDNED G+LIAMA
Sbjct: 61  DACQNLMNKPEQSSNETSPFVLIVRGGCSFEEKVRKAQRAGFKAAIIYDNEDRGTLIAMA 120

Query: 126 GSSAGIRIHAVFVTKSSGETLKKYAGLANVEIWIVPSFENSAWSIMAISFISLLAMSAVL 185
           G+S GIRIHAVFVTK +GE LK+YAG  + ++W++PSFENSAWSIMA+SFISLLAMSAVL
Sbjct: 121 GNSGGIRIHAVFVTKETGEVLKEYAGFPDTKVWLIPSFENSAWSIMAVSFISLLAMSAVL 180

Query: 186 ATCFFVRRHRIRRERPRSSHSQEFRGMSRRVVKAMPSLIFTTALEDNCTSVTCAICLEDY 245
           ATCFFVRRHRIRR   RSS  +EF GMSRR+VKAMPSLIF++  EDN T+ TCAICLEDY
Sbjct: 181 ATCFFVRRHRIRRRTSRSSRVREFHGMSRRLVKAMPSLIFSSFHEDNTTAFTCAICLEDY 240

Query: 246 TAGEKLRILPCRHSPGYMKLQHQGCPSFLAKEFHAFCVDSWLTAWRTFCPVCKRDARTST 305
           T G+KLR+LPC H                  +FHA CVDSWLT+WRTFCPVCKRDARTST
Sbjct: 241 TVGDKLRLLPCCH------------------KFHAACVDSWLTSWRTFCPVCKRDARTST 300

Query: 306 GDPPPSESTPLLSSTPNSVASSSILSSVRSSLPPSSAIQISPS--SRSPSVSRNHSLSST 365
           G+PP SESTPLLSS  +S  SSS+ SSVR     SSA+ I PS  S   S+S + + +S+
Sbjct: 301 GEPPASESTPLLSSAASSFTSSSLHSSVR-----SSALLIGPSLGSLPTSISFSPAYASS 360

Query: 366 PYHQQSLRSSYH-QSPSLSVSRSSVELRN-------ASSQRSYASNLVSPYSSCYPSMSP 425
            Y +QS +SS + +SP +SVSRSSV+LR        + SQRSY S++ SP S  YP++SP
Sbjct: 361 SYIRQSFQSSSNRRSPPISVSRSSVDLRQQAASPSPSPSQRSYISHMASPQSLGYPTISP 420

Query: 426 LHSRYMIPLIPSPSNASPSIFGSSSHQQNPLHISGSAASFSPFASARSLPEC 468
            ++RYM P  PSPSNASP++ GSS++  NPL  S SA +FSP+ASA SLP+C
Sbjct: 421 FNTRYMSPYRPSPSNASPAMAGSSNYPLNPLRYSESAGTFSPYASANSLPDC 448

BLAST of Spg039975 vs. TAIR 10
Match: AT4G09560.1 (Protease-associated (PA) RING/U-box zinc finger family protein )

HSP 1 Score: 356.3 bits (913), Expect = 3.8e-98
Identity = 228/475 (48.00%), Postives = 295/475 (62.11%), Query Frame = 0

Query: 6   MNRGLALLFSLLLVGCCGSAANVVLIGTNVTLSFDSIEANFAPTIESSGEPGVLYLAKPL 65
           M R   ++ SLLL+    S A V+LIG + +LSFD +EA F P I+ S + GVLY+A+PL
Sbjct: 1   MIRSSIVILSLLLISHLVS-AKVLLIGNSTSLSFDDVEATFTPMIKRSDQGGVLYVAEPL 60

Query: 66  DACSTLENKVRLP--VNSSSPFALIIRGGCSFEDKVRRAQVAGFKAAIVYDNEDSGSLIA 125
           DACS L N V +      S P+ LIIRGGCSFEDK+R AQ AG+KAAIVYD ED G L++
Sbjct: 61  DACSDLVNTVNVKNGTTVSPPYVLIIRGGCSFEDKIRNAQKAGYKAAIVYDYEDFGFLVS 120

Query: 126 MAGSSAGIRIHAVFVTKSSGETLKKYAGLANVEIWIVPSFENSAWSIMAISFISLLAMSA 185
           MAG+ +G+ I+  FV+K++GE LK+YAG  + E+W++PSFE SAWSIMAISFISLLAMSA
Sbjct: 121 MAGNPSGVLIYGTFVSKATGEVLKEYAGRTDFEVWLMPSFETSAWSIMAISFISLLAMSA 180

Query: 186 VLATCFFVRRHRIRRERPRSSHSQEFRGMSRRVVKAMPSLIFTTALEDNCTSVTCAICLE 245
           VLATCFFVRRHR+RR R  + +  +F  M + ++  MP+ IF    ++  TS+ C ICLE
Sbjct: 181 VLATCFFVRRHRVRRRRILALNGNDFHRMPKSMIIRMPTTIFNGICDEATTSILCCICLE 240

Query: 246 DYTAGEKLRILPCRHSPGYMKLQHQGCPSFLAKEFHAFCVDSWLTAWRTFCPVCKRDART 305
           +Y  G+KLRILPC H                  +FH  CVD WL   ++FCPVCKRDAR+
Sbjct: 241 NYEKGDKLRILPCHH------------------KFHVACVDLWLGQRKSFCPVCKRDARS 300

Query: 306 STGDPPPSESTPLLSSTPNSVASSSILSSVRSSLPPSS------AIQISPSSRSPSVS-- 365
            + D PPSE TP LS TP+   +SS L S  S+ P  S      +I++ PS  S S+   
Sbjct: 301 ISTDKPPSEHTPFLSRTPSMTPTSSFLLSSSSTTPLQSSHELPISIRVDPSLPSTSMQPH 360

Query: 366 -----RNHSLSSTPYHQQSLRSSYHQSPSLSVSRSSVELRNASSQRSYASNLVSPYSSCY 425
                 +HS S T +   S R     S  + VSRSS +LRNA SQRSY     SP+    
Sbjct: 361 TVPMYLSHSRSHTSFQNGSNRF----SRPIPVSRSSADLRNAVSQRSYN----SPHQVSL 420

Query: 426 PSMSPLHSRYMIPLIPSPSNASPSIFGSSSHQQNPLHISGSAASFSPFASARSLP 466
           P    LHSRY   L P  ++ S  +   +S +++ LH + S  SF  FASA SLP
Sbjct: 421 PRF--LHSRYTHILGPGNASRSQVVGLLTSQREHSLHQNDSRRSFIHFASASSLP 446

BLAST of Spg039975 vs. TAIR 10
Match: AT1G22670.1 (Protease-associated (PA) RING/U-box zinc finger family protein )

HSP 1 Score: 307.8 bits (787), Expect = 1.5e-83
Identity = 216/466 (46.35%), Postives = 283/466 (60.73%), Query Frame = 0

Query: 6   MNRGLALLFSLLL--VGCCGSAANVVLIGTNVTLSFDSIEANFAPTIESSGEPGVLYLAK 65
           MN  + L+ +LLL  V     A  V+L+ +N+T SF  +EA+F+P++ ++ E GV+Y+A+
Sbjct: 1   MNLVVLLILTLLLFIVSYVVDAGQVILVDSNITRSFVDMEADFSPSV-TTVETGVVYVAE 60

Query: 66  PLDACSTLENKVRLPVNSSSPFALIIRGGCSFEDKVRRAQVAGFKAAIVYDNEDSGSLIA 125
           PL+AC  L NK       +SP  LIIRGGCSFE KVR AQ +GFKAAIVYDN D   L A
Sbjct: 61  PLNACRNLRNKPEQSPYGTSPLVLIIRGGCSFEYKVRNAQRSGFKAAIVYDNVDRNFLSA 120

Query: 126 MAGSSAGIRIHAVFVTKSSGETLKKYAGLANVEIWIV-PSFENSAWSIMA-ISFISLLAM 185
           M G S GI+I AVFV K +GE LKKYAG   +E+ +V P+ E+S WS+ A I+ I  LA+
Sbjct: 121 MGGDSDGIKIQAVFVMKRAGEMLKKYAGSEEMEVMLVPPNTEDSVWSLYASIALILSLAI 180

Query: 186 SAVLATCFFVRRHRIRRERPRSSHSQEFRGMSRRVVKAMPSLIFTTALEDNCTSVTCAIC 245
             V+ TC F  R+       R+S SQ F GM RR VKAMPS+ FT A  DN T  +CAIC
Sbjct: 181 FCVMVTCVFFYRY---CSTIRNSTSQ-FNGMCRRTVKAMPSVTFTCAKIDNTTGFSCAIC 240

Query: 246 LEDYTAGEKLRILPCRHSPGYMKLQHQGCPSFLAKEFHAFCVDSWLTAWRTFCPVCKRDA 305
           LEDY  G+KLR+LPC H                  +FH  CVDSWL +WRTFCPVCKRDA
Sbjct: 241 LEDYIVGDKLRVLPCSH------------------KFHVACVDSWLISWRTFCPVCKRDA 300

Query: 306 RTSTGDPPPSESTPLLSSTPNSVASSSILSSVRSSLPPSSAIQISPSSRSPSVSRNHSLS 365
           RT+  +P  +ESTP LSS   S+A+SS++     S P  S++  SP+  S S        
Sbjct: 301 RTTADEPLATESTPFLSS---SIATSSLVCI--DSPPLGSSVSFSPAHVSSS-------- 360

Query: 366 STPYHQQSLRSSYHQSPSLSVS-RSSVELRNASSQRSYASNLVSPYSSCYPSMSPLHSRY 425
              +  Q +RSS      +S + R       +SSQRS+ S + S +S  Y +MSPL++  
Sbjct: 361 ---FIHQFVRSSPMNGSRISENLRRQASPLQSSSQRSHLS-MKSSHSLGYSTMSPLNAMG 420

Query: 426 MIPLIPSPSNASPSIFGSSSHQQNPLHISGSAASFSPFASARSLPE 467
           M P  P PSNASP +F S++H    L  + +A +FS FASA SLP+
Sbjct: 421 MSPYRPYPSNASPGLFSSTNH----LLSNYTANTFSHFASAHSLPD 422

BLAST of Spg039975 vs. TAIR 10
Match: AT1G35630.1 (Protease-associated (PA) RING/U-box zinc finger family protein )

HSP 1 Score: 299.7 bits (766), Expect = 4.2e-81
Identity = 158/322 (49.07%), Postives = 218/322 (67.70%), Query Frame = 0

Query: 6   MNRGLALLFSLLLVGCCGSAANVVLIGTNVTLSFDSIEANFAPTIESSGEPGVLYLAKPL 65
           MN     + SLL++ C  ++A VVLIG N  LSFD +EA F P + +SGE G+LY+A+PL
Sbjct: 1   MNYSWITIMSLLVI-CKLASAKVVLIGKNTILSFDDVEATFTPIVRNSGECGILYVAEPL 60

Query: 66  DACSTLENKVRLPVNSSSPFALIIRGGCSFEDKVRRAQVAGFKAAIVYDNEDSGSLIAMA 125
           +ACS + N         S + LI+ GGCSFE+KVR+AQ AG+KAAIVY++     L+ MA
Sbjct: 61  EACSDITNMAEKRSKYRSSYVLIVLGGCSFEEKVRKAQKAGYKAAIVYNDGYDELLVPMA 120

Query: 126 GSSAGIRIHAVFVTKSSGETLKKYAGLANVEIWIVPSFENSAWSIMAISFISLLAMSAVL 185
           G+S+G+ IH + VT++SGE LK YA    +++W++P F  S+WSIM I+FISLLAMSA+L
Sbjct: 121 GNSSGVDIHGLLVTRASGEVLKGYADQDEMKLWLIPGFGISSWSIMGITFISLLAMSAIL 180

Query: 186 ATCFFVRRHRIRRE-RPRSSHSQEFRGMSRRVVKAMPSLIFTTALEDNCTSVTCAICLED 245
           ATCF VRRH+IR+  R      Q    M R ++++MP+ +++  LE++ TSVTCAIC++D
Sbjct: 181 ATCFVVRRHQIRQSVRDLPHGGQGLSCMPRDLLQSMPTEVYSGVLEESSTSVTCAICIDD 240

Query: 246 YTAGEKLRILPCRHSPGYMKLQHQGCPSFLAKEFHAFCVDSWLTAWRTFCPVCKRDARTS 305
           Y  GEKLRILPC+H                  ++HA C+DSWL   R+FCPVCK++ RT 
Sbjct: 241 YCVGEKLRILPCKH------------------KYHAVCIDSWLGRCRSFCPVCKQNPRTG 300

Query: 306 TGDPPPSESTPLLSSTPNSVAS 327
              PP SE+TPL+S +PNS+ S
Sbjct: 301 NDVPPASETTPLISPSPNSITS 303

BLAST of Spg039975 vs. TAIR 10
Match: AT5G66160.2 (receptor homology region transmembrane domain ring H2 motif protein 1 )

HSP 1 Score: 191.0 bits (484), Expect = 2.1e-48
Identity = 123/295 (41.69%), Postives = 162/295 (54.92%), Query Frame = 0

Query: 13  LFSLLLVGCCGSAANVVLIGTNVTLSFDSIEANFAPTIESSGEPGVLYLAKPLDACSTLE 72
           L SLL V    S A VVL   +++ SF  + A F  ++  +G  G LY+A PLD CS L 
Sbjct: 16  LSSLLRV----SLATVVL--NSISASFADLPAKFDGSVTKNGICGALYVADPLDGCSPLL 75

Query: 73  NKV--RLPVNSSSPFALIIRGGCSFEDKVRRAQVAGFKAAIVYDNEDSGSLIAMAGSSAG 132
           +        + ++ FALIIRG CSFEDK+  AQ +GF+A IVYDN D+  LI M  +   
Sbjct: 76  HAAASNWTQHRTTKFALIIRGECSFEDKLLNAQNSGFQAVIVYDNIDNEDLIVMKVNPQD 135

Query: 133 IRIHAVFVTKSSGETLKKYAGLANVEIWIVPSFENSAWSIMAISFISLLAMSAVLATCFF 192
           I + AVFV+  +GE L+KYA   + E  + P    SAW+++AISF SLL +   L   FF
Sbjct: 136 ITVDAVFVSNVAGEILRKYARGRDGECCLNPPDRGSAWTVLAISFFSLLLIVTFLLIAFF 195

Query: 193 VRRHRIRRERPRSSHSQEFRGMSRRVVKAMPSLIFTTALEDNCTSVTCAICLEDYTAGEK 252
             RH  +    R  H++  R +  ++V  +P   FT +        TCAICLEDY  GE 
Sbjct: 196 APRHWTQW---RGRHTRTIR-LDAKLVHTLPCFTFTDSAHHK-AGETCAICLEDYRFGES 255

Query: 253 LRILPCRHSPGYMKLQHQGCPSFLAKEFHAFCVDSWLTAWRTFCPVCKRDARTST 306
           LR+LPC+H+                  FH  C+DSWLT W T CPVCK D RT T
Sbjct: 256 LRLLPCQHA------------------FHLNCIDSWLTKWGTSCPVCKHDIRTET 281

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022939223.15.6e-22189.51receptor homology region, transmembrane domain- and RING domain-containing prote... [more]
KAG6578333.15.6e-22189.51Receptor-likey region, transmembrane domain- and RING domain-containing protein ... [more]
XP_038885132.18.1e-22089.29receptor homology region, transmembrane domain- and RING domain-containing prote... [more]
XP_023550607.11.1e-21989.08receptor homology region, transmembrane domain- and RING domain-containing prote... [more]
XP_022993427.11.5e-21888.65receptor homology region, transmembrane domain- and RING domain-containing prote... [more]
Match NameE-valueIdentityDescription
Q8VZ141.5e-14461.23Receptor homology region, transmembrane domain- and RING domain-containing prote... [more]
Q10R934.3e-11556.70Receptor homology region, transmembrane domain- and RING domain-containing prote... [more]
Q69U496.0e-10953.67Receptor homology region, transmembrane domain- and RING domain-containing prote... [more]
Q0WPW55.3e-9748.00Receptor homology region, transmembrane domain- and RING domain-containing prote... [more]
F4I2Y32.2e-8246.35Receptor homology region, transmembrane domain- and RING domain-containing prote... [more]
Match NameE-valueIdentityDescription
A0A6J1FL262.7e-22189.51receptor homology region, transmembrane domain- and RING domain-containing prote... [more]
A0A6J1JSS37.4e-21988.65receptor homology region, transmembrane domain- and RING domain-containing prote... [more]
A0A6J1DE262.2e-21888.22receptor homology region, transmembrane domain- and RING domain-containing prote... [more]
A0A1S3B2Y06.7e-21286.51receptor homology region, transmembrane domain- and RING domain-containing prote... [more]
A0A1S3B2Z22.2e-21085.50receptor homology region, transmembrane domain- and RING domain-containing prote... [more]
Match NameE-valueIdentityDescription
AT1G71980.11.1e-14561.23Protease-associated (PA) RING/U-box zinc finger family protein [more]
AT4G09560.13.8e-9848.00Protease-associated (PA) RING/U-box zinc finger family protein [more]
AT1G22670.11.5e-8346.35Protease-associated (PA) RING/U-box zinc finger family protein [more]
AT1G35630.14.2e-8149.07Protease-associated (PA) RING/U-box zinc finger family protein [more]
AT5G66160.22.1e-4841.69receptor homology region transmembrane domain ring H2 motif protein 1 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 197..303
e-value: 2.9E-15
score: 57.7
IPR003137PA domainPFAMPF02225PAcoord: 85..146
e-value: 1.4E-7
score: 31.5
NoneNo IPR availableGENE3D3.50.30.30coord: 26..160
e-value: 1.8E-25
score: 91.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 301..323
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 335..378
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 306..323
NoneNo IPR availablePANTHERPTHR22765:SF308PROTEASE-ASSOCIATED (PA) RING/U-BOX ZINC FINGER PROTEINcoord: 10..400
NoneNo IPR availablePANTHERPTHR22765RING FINGER AND PROTEASE ASSOCIATED DOMAIN-CONTAININGcoord: 10..400
NoneNo IPR availableCDDcd16486mRING-H2-C3H2C2D_ZSWM2coord: 237..298
e-value: 1.02215E-18
score: 77.2669
NoneNo IPR availableSUPERFAMILY52025PA domaincoord: 76..163
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 231..312
IPR001841Zinc finger, RING-typePFAMPF17123zf-RING_11coord: 238..258
e-value: 2.6E-6
score: 27.1
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 238..298
score: 10.084539
IPR044744ZNRF4 /RNF13/RNF167, PA domainCDDcd02123PA_C_RZF_likecoord: 24..167
e-value: 2.90401E-50
score: 166.361

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg039975.1Spg039975.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015031 protein transport
biological_process GO:0016567 protein ubiquitination
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005773 vacuole
molecular_function GO:0046872 metal ion binding
molecular_function GO:0061630 ubiquitin protein ligase activity