Spg039203 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg039203
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptioncinnamoyl-CoA reductase-like SNL6
Locationscaffold10: 43319963 .. 43322539 (-)
RNA-Seq ExpressionSpg039203
SyntenySpg039203
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTCTCTCTCTCTCTCTCTCTCTAGATTTTCAGTGTAATCGGCGAAATGGGCGTTGTAATTCGTTCCGAAGAGACCTTCTTATCGGAGCTGGAGCAGCTCCGCCGCAAGCTCGTCGCGTGCGGCGGCGTCCACCGGAGGAAGGACGGCGAAGAATTTAAAAGCCTCAAACCGAAGCCGATCGACGATGCAGAAGAGAAAGTCGTGTGTGTAACCAGCGGCGTTTCGTTTCTCGGTTCCGCCATCGTTAACGAGCTCTCGATTCACGGATACTCTGTTCGTGTCATTGTTGATAATCCTGGTGAGTTAATTGAAGTTCTCTGATTCTTCGATTTGAACTTGAATTGCATGTATCGAGTTGCGTAATCGATCGATTTCAATTGCTAAAGATTGGTCGAAATGGAAGGTTGAGTTGTTGTTTTGGATCATAGACTAATGTAATACCTAAAAATGGCAGAGGATGTGAACAAATTGGAAGAGACGACGAGCGGCCGCCATAACGTGACGGTGGTTGTAGCGAAGCTTACGGACGTAAATAGCTTAGTGGAAGCATTCAATGGCTGCCGTGGCGTTTTTCACACCTCTTCCTCCATTGATCCTGCTGGTGTTTCTGGTTACACGGTGAGGGCTTGTTCTTTACCTTTCTTAGAAATGAAGTTGCTGCTATTATATGAACCTCCCACCCTGTCTTCATCGCATCAGATATGGCAGTTTTGAATTTGTTTTTTTGCATTTGCTTGAGACCTACGATTGCTTTCATTGTCAATGTCAAGAAAGCTTAGCATATGCTCCGGTTTACACGCCAAAACTTTCAAATTATTCCTCTAAATTGACCTTGAAACAAATAATTGATGTGTTCTTATTCTCATGCATCATATATATGTTTTTTCTCACCATAAATATGGAAATGGAGATTTGAACCTCAACTTCAAGAATAAGAATGCATGCTTCAACAGTAGAACGTCTATACGCGTTAGTCAATACATGTTTTTCTAGTCAACATAAGAATATATCTCTTTTACAAAATTTTAAAATGAAATCAAACATTGTTGAATGGTAAATTTGCAACTCTTATTCAAGATTAAAGTCAATATTGAAACACTTATCAAATTCTAAGGTATCGTTTTAATGGTTAAGTTTAGGGTCAAAATTGATTTATTCACCGTGTTGTTTTAAGTTTTCTAAAAGAGAATTGTGGATTGTTGTTTGGTACAGAAAGTAATGAGCGAAGTGGAAATGAGGACCACTGAGAACGTGATGGAGGCGTGCGCAAGAACAGAGACAGTAAAGAATTGTGTTCTTACATCTTCTCTTCTTGCTTGCATCTGGCGGGACCAAACTGGTGAAAGTGAAAGCACTGGAGCCTCACGAATTGTCAACCATGGCAGCTGGAGCAGCTTACAGCTCTGCCAAGACAAAAAGGTCAGTCCCTTTCTTTCTCCTTGCTCCACCATCATGAATTTCAACACCATCATGATTTTAAAACTACTGTCAAAGAATGGTGAGAAAAAGAACACAAGTCAAACGAGACCTTAGAACTAGATGGAAAAATTGAAACCAAGAGTAATTTTTACAATCTACCGAGAGACAGAGTTTTATGTTGATGGGGTTTCTAACAAACAAGTTTGACTATTATGCAGCTATGGTTTGCATTGGGGAAACTAAAATCTGAAAAAGTGGCATGGAAAATCGCACAAGAAAGTGGCCTAAAACTGGCCACCATCTGTTCTGCTCTCGTAACTGGCCCCAACTTTCATCGCAGAAATTCAACTCCAACAATGGCTTATCTCAAAGGTAAAACTTCAACAACCAAAAGAACTTCCATTCCCCATCCATTAACAAGAACACATATGCATATTGAACTCAAAATCTTCATTGTTGTCAATTCAGGAGCTCGAGAGATGTACACAAACAGTCTACTGGCAACGGTGGACGTGACCAAATTGGCCAAAGCACATGTCTGTGTGTATGAGGGAATGAAGGAAAATGCTTCCGGCAGATACATTTGCTTCGACCGTGTGATCTCGACTGTCGACGAGGCGACGAAATTGGCGTCGGAAATCGGAGTCCCGGTCGGGACCATAGTCCAAACCACCCCTCCGATGGACTCTCCGATCTCGTTTCAATTGTCGAACAAGAAGCTCTCTGATCTAATGTCCAGAAGTAACCTTCAACGTAGCTGTTTGAATGAAAACAGATTTTCTTGAAGGATAAAGAACTTGCATCTTCACTGGCCAAGAGCAATTTGCAAGCTAAAAATATTAGTGGAATCTATTCATTTATTAAATTTGATTAAAAATAGTCTTAAAAAAGTAGATTGCTATTAGAAATGAGCTCTTGATGAGGATGTATTATTCTTTCTTTTAAAGTATCATTGATATAGAATGTAAGATGTATACCATTTTTTGTTACTTTGTTTATATTGAAGAGAGAAAGTGCAATTTTTTTTATTCTCCTACTTATAATTGTCTTTGTAAAAATGTAAAGAAAAATGAAACCGATAAATATGTAACTTGGCTGCATATGAGTATGTATGGATCTCCCTACATACCACCATTTAAAGGTTGATAACTAGGAGAG

mRNA sequence

ATGGGCGTTGTAATTCGTTCCGAAGAGACCTTCTTATCGGAGCTGGAGCAGCTCCGCCGCAAGCTCGTCGCGTGCGGCGGCGTCCACCGGAGGAAGGACGGCGAAGAATTTAAAAGCCTCAAACCGAAGCCGATCGACGATGCAGAAGAGAAAGTCGTGTGTGTAACCAGCGGCGTTTCGTTTCTCGGTTCCGCCATCGTTAACGAGCTCTCGATTCACGGATACTCTGTTCGTGTCATTGTTGATAATCCTGAGGATGTGAACAAATTGGAAGAGACGACGAGCGGCCGCCATAACGTGACGGTGGTTGTAGCGAAGCTTACGGACGTAAATAGCTTAGTGGAAGCATTCAATGGCTGCCGTGGCGTTTTTCACACCTCTTCCTCCATTGATCCTGCTGGTGTTTCTGGTTACACGAAAGTAATGAGCGAAGTGGAAATGAGGACCACTGAGAACGTGATGGAGGCGTGCGCAAGAACAGAGACAGTAAAGAATTGTGTTCTTACATCTTCTCTTCTTGCTTGCATCTGGCGGGACCAAACTGGTGAAAGTGAAAGCACTGGAGCCTCACGAATTGTCAACCATGGCAGCTGGAGCAGCTTACAGCTCTGCCAAGACAAAAAGCTATGGTTTGCATTGGGGAAACTAAAATCTGAAAAAGTGGCATGGAAAATCGCACAAGAAAGTGGCCTAAAACTGGCCACCATCTGTTCTGCTCTCGTAACTGGCCCCAACTTTCATCGCAGAAATTCAACTCCAACAATGGCTTATCTCAAAGGAGCTCGAGAGATGTACACAAACAGTCTACTGGCAACGGTGGACGTGACCAAATTGGCCAAAGCACATGTCTGTGTGTATGAGGGAATGAAGGAAAATGCTTCCGGCAGATACATTTGCTTCGACCGTGTGATCTCGACTGTCGACGAGGCGACGAAATTGGCGTCGGAAATCGGAGTCCCGGTCGGGACCATAGTCCAAACCACCCCTCCGATGGACTCTCCGATCTCGTTTCAATTGTCGAACAAGAAGCTCTCTGATCTAATGTCCAGAAGTAACCTTCAACGTAGCTGTTTGAATGAAAACAGATTTTCTTGA

Coding sequence (CDS)

ATGGGCGTTGTAATTCGTTCCGAAGAGACCTTCTTATCGGAGCTGGAGCAGCTCCGCCGCAAGCTCGTCGCGTGCGGCGGCGTCCACCGGAGGAAGGACGGCGAAGAATTTAAAAGCCTCAAACCGAAGCCGATCGACGATGCAGAAGAGAAAGTCGTGTGTGTAACCAGCGGCGTTTCGTTTCTCGGTTCCGCCATCGTTAACGAGCTCTCGATTCACGGATACTCTGTTCGTGTCATTGTTGATAATCCTGAGGATGTGAACAAATTGGAAGAGACGACGAGCGGCCGCCATAACGTGACGGTGGTTGTAGCGAAGCTTACGGACGTAAATAGCTTAGTGGAAGCATTCAATGGCTGCCGTGGCGTTTTTCACACCTCTTCCTCCATTGATCCTGCTGGTGTTTCTGGTTACACGAAAGTAATGAGCGAAGTGGAAATGAGGACCACTGAGAACGTGATGGAGGCGTGCGCAAGAACAGAGACAGTAAAGAATTGTGTTCTTACATCTTCTCTTCTTGCTTGCATCTGGCGGGACCAAACTGGTGAAAGTGAAAGCACTGGAGCCTCACGAATTGTCAACCATGGCAGCTGGAGCAGCTTACAGCTCTGCCAAGACAAAAAGCTATGGTTTGCATTGGGGAAACTAAAATCTGAAAAAGTGGCATGGAAAATCGCACAAGAAAGTGGCCTAAAACTGGCCACCATCTGTTCTGCTCTCGTAACTGGCCCCAACTTTCATCGCAGAAATTCAACTCCAACAATGGCTTATCTCAAAGGAGCTCGAGAGATGTACACAAACAGTCTACTGGCAACGGTGGACGTGACCAAATTGGCCAAAGCACATGTCTGTGTGTATGAGGGAATGAAGGAAAATGCTTCCGGCAGATACATTTGCTTCGACCGTGTGATCTCGACTGTCGACGAGGCGACGAAATTGGCGTCGGAAATCGGAGTCCCGGTCGGGACCATAGTCCAAACCACCCCTCCGATGGACTCTCCGATCTCGTTTCAATTGTCGAACAAGAAGCTCTCTGATCTAATGTCCAGAAGTAACCTTCAACGTAGCTGTTTGAATGAAAACAGATTTTCTTGA

Protein sequence

MGVVIRSEETFLSELEQLRRKLVACGGVHRRKDGEEFKSLKPKPIDDAEEKVVCVTSGVSFLGSAIVNELSIHGYSVRVIVDNPEDVNKLEETTSGRHNVTVVVAKLTDVNSLVEAFNGCRGVFHTSSSIDPAGVSGYTKVMSEVEMRTTENVMEACARTETVKNCVLTSSLLACIWRDQTGESESTGASRIVNHGSWSSLQLCQDKKLWFALGKLKSEKVAWKIAQESGLKLATICSALVTGPNFHRRNSTPTMAYLKGAREMYTNSLLATVDVTKLAKAHVCVYEGMKENASGRYICFDRVISTVDEATKLASEIGVPVGTIVQTTPPMDSPISFQLSNKKLSDLMSRSNLQRSCLNENRFS
Homology
BLAST of Spg039203 vs. NCBI nr
Match: KAG7016195.1 (Cinnamoyl-CoA reductase-like SNL6 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 641.7 bits (1654), Expect = 3.7e-180
Identity = 322/360 (89.44%), Postives = 343/360 (95.28%), Query Frame = 0

Query: 1   MGVVIRSEETFLSELEQLRRKLVACGGVHRRKDGEEFKSLKPKPIDDAEEKVVCVTSGVS 60
           MGVV+RSEETF+SELE+ RR LVAC GV RRKDG+EFKSLKPKPIDDA+EKVVCVTSGVS
Sbjct: 1   MGVVVRSEETFVSELERCRRMLVACAGVQRRKDGKEFKSLKPKPIDDADEKVVCVTSGVS 60

Query: 61  FLGSAIVNELSIHGYSVRVIVDNPEDVNKLEETTSGRHNVTVVVAKLTDVNSLVEAFNGC 120
           FLGSAIVNELS+HGY VRVIVDNPEDVNKLEETTSGRHNVTVVVA LTDVNSLVEAFNGC
Sbjct: 61  FLGSAIVNELSLHGYFVRVIVDNPEDVNKLEETTSGRHNVTVVVADLTDVNSLVEAFNGC 120

Query: 121 RGVFHTSSSIDPAGVSGYTKVMSEVEMRTTENVMEACARTETVKNCVLTSSLLACIWRDQ 180
           RGVFHTSSSIDP+GVSGYTKVMSEVEMRTTENVMEACARTETV+NCVLTSSLLACIWRDQ
Sbjct: 121 RGVFHTSSSIDPSGVSGYTKVMSEVEMRTTENVMEACARTETVRNCVLTSSLLACIWRDQ 180

Query: 181 TGESESTGASRIVNHGSWSSLQLCQDKKLWFALGKLKSEKVAWKIAQESGLKLATICSAL 240
            GESES G+SRIV+H  WSSLQLCQDKKLWFALGKLKSEKVAWKIAQESGLKLATICSAL
Sbjct: 181 AGESESIGSSRIVDHACWSSLQLCQDKKLWFALGKLKSEKVAWKIAQESGLKLATICSAL 240

Query: 241 VTGPNFHRRNSTPTMAYLKGAREMYTNSLLATVDVTKLAKAHVCVYEGMKENASGRYICF 300
           +TGPNFHRR+ST T+AYLKGAREMYTN LLATVDVTKLAKAHVCVYEGMKEN+S RYICF
Sbjct: 241 ITGPNFHRRSSTATIAYLKGAREMYTNGLLATVDVTKLAKAHVCVYEGMKENSSSRYICF 300

Query: 301 DRVISTVDEATKLASEIGVPVGTIVQTTPPMDSPISFQLSNKKLSDLMSRSNLQRSCLNE 360
           DRVIST +EA+ LA+EIGVPVG+IVQTTPP++SP+SFQLSNKKL DLM RSNLQRSCLNE
Sbjct: 301 DRVISTANEASMLATEIGVPVGSIVQTTPPINSPVSFQLSNKKLCDLMFRSNLQRSCLNE 360

BLAST of Spg039203 vs. NCBI nr
Match: XP_022938554.1 (cinnamoyl-CoA reductase-like SNL6 [Cucurbita moschata])

HSP 1 Score: 640.6 bits (1651), Expect = 8.3e-180
Identity = 321/360 (89.17%), Postives = 343/360 (95.28%), Query Frame = 0

Query: 1   MGVVIRSEETFLSELEQLRRKLVACGGVHRRKDGEEFKSLKPKPIDDAEEKVVCVTSGVS 60
           MGVV+RSEETF+SELE+ RR LVAC GV RRKDG+EFKSLKP+PIDDA+EKVVCVTSGVS
Sbjct: 1   MGVVVRSEETFVSELERCRRMLVACAGVQRRKDGKEFKSLKPRPIDDADEKVVCVTSGVS 60

Query: 61  FLGSAIVNELSIHGYSVRVIVDNPEDVNKLEETTSGRHNVTVVVAKLTDVNSLVEAFNGC 120
           FLGSAIVNELS+HGY VRVIVDNPEDVNKLEETTSGRHNVTVVVA LTDVNSLVEAFNGC
Sbjct: 61  FLGSAIVNELSLHGYFVRVIVDNPEDVNKLEETTSGRHNVTVVVADLTDVNSLVEAFNGC 120

Query: 121 RGVFHTSSSIDPAGVSGYTKVMSEVEMRTTENVMEACARTETVKNCVLTSSLLACIWRDQ 180
           RGVFHTSSSIDP+GVSGYTKVMSEVEMRTTENVMEACARTETV+NCVLTSSLLACIWRDQ
Sbjct: 121 RGVFHTSSSIDPSGVSGYTKVMSEVEMRTTENVMEACARTETVRNCVLTSSLLACIWRDQ 180

Query: 181 TGESESTGASRIVNHGSWSSLQLCQDKKLWFALGKLKSEKVAWKIAQESGLKLATICSAL 240
            GESES G+SRIV+H  WSSLQLCQDKKLWFALGKLKSEKVAWKIAQESGLKLATICSAL
Sbjct: 181 AGESESIGSSRIVDHACWSSLQLCQDKKLWFALGKLKSEKVAWKIAQESGLKLATICSAL 240

Query: 241 VTGPNFHRRNSTPTMAYLKGAREMYTNSLLATVDVTKLAKAHVCVYEGMKENASGRYICF 300
           +TGPNFHRR+ST T+AYLKGAREMYTN LLATVDVTKLAKAHVCVYEGMKEN+S RYICF
Sbjct: 241 ITGPNFHRRSSTATIAYLKGAREMYTNGLLATVDVTKLAKAHVCVYEGMKENSSSRYICF 300

Query: 301 DRVISTVDEATKLASEIGVPVGTIVQTTPPMDSPISFQLSNKKLSDLMSRSNLQRSCLNE 360
           DRVIST +EA+ LA+EIGVPVG+IVQTTPP++SP+SFQLSNKKL DLM RSNLQRSCLNE
Sbjct: 301 DRVISTANEASMLATEIGVPVGSIVQTTPPINSPVSFQLSNKKLCDLMFRSNLQRSCLNE 360

BLAST of Spg039203 vs. NCBI nr
Match: XP_023551091.1 (cinnamoyl-CoA reductase-like SNL6 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 639.8 bits (1649), Expect = 1.4e-179
Identity = 322/360 (89.44%), Postives = 343/360 (95.28%), Query Frame = 0

Query: 1   MGVVIRSEETFLSELEQLRRKLVACGGVHRRKDGEEFKSLKPKPIDDAEEKVVCVTSGVS 60
           MGVV+RSEETF+SELE+ RR LVAC GV RRKDG+EFKSLK KPIDDA+EKVVCVTSGVS
Sbjct: 1   MGVVVRSEETFVSELERCRRMLVACAGVQRRKDGKEFKSLKLKPIDDADEKVVCVTSGVS 60

Query: 61  FLGSAIVNELSIHGYSVRVIVDNPEDVNKLEETTSGRHNVTVVVAKLTDVNSLVEAFNGC 120
           FLGSAIVNELS+HGY VRVIVDNPEDVNKLEETTSGRHNVTVVVA LTDVNSLVEAFNGC
Sbjct: 61  FLGSAIVNELSLHGYFVRVIVDNPEDVNKLEETTSGRHNVTVVVADLTDVNSLVEAFNGC 120

Query: 121 RGVFHTSSSIDPAGVSGYTKVMSEVEMRTTENVMEACARTETVKNCVLTSSLLACIWRDQ 180
           RGVFHTSSSIDP+GVSGYTKVMSEVE+RTTENVMEACARTETV+NCVLTSSLLACIWRDQ
Sbjct: 121 RGVFHTSSSIDPSGVSGYTKVMSEVEVRTTENVMEACARTETVRNCVLTSSLLACIWRDQ 180

Query: 181 TGESESTGASRIVNHGSWSSLQLCQDKKLWFALGKLKSEKVAWKIAQESGLKLATICSAL 240
            GESES G+SRIV+H  WSSLQLCQDKKLWFALGKLKSEKVAWKIAQESGLKLATICSAL
Sbjct: 181 AGESESIGSSRIVDHACWSSLQLCQDKKLWFALGKLKSEKVAWKIAQESGLKLATICSAL 240

Query: 241 VTGPNFHRRNSTPTMAYLKGAREMYTNSLLATVDVTKLAKAHVCVYEGMKENASGRYICF 300
           +TGPNFHRR+STPT+AYLKGAREMYTN LLATVDVTKLAKAHVCVYEGMKEN+S RYICF
Sbjct: 241 ITGPNFHRRSSTPTIAYLKGAREMYTNGLLATVDVTKLAKAHVCVYEGMKENSSSRYICF 300

Query: 301 DRVISTVDEATKLASEIGVPVGTIVQTTPPMDSPISFQLSNKKLSDLMSRSNLQRSCLNE 360
           DRVIST +EAT LA+EIGVPVG+IVQTTP ++SP+SFQLSNKKLSDLM RSNLQRSCLNE
Sbjct: 301 DRVISTANEATMLAAEIGVPVGSIVQTTPTINSPVSFQLSNKKLSDLMFRSNLQRSCLNE 360

BLAST of Spg039203 vs. NCBI nr
Match: XP_022993633.1 (cinnamoyl-CoA reductase-like SNL6 [Cucurbita maxima])

HSP 1 Score: 637.1 bits (1642), Expect = 9.2e-179
Identity = 322/364 (88.46%), Postives = 344/364 (94.51%), Query Frame = 0

Query: 1   MGVVIRSEETFLSELEQLRRKLVACGGVHRRKDGEEFKSLKPKPIDDAEEKVVCVTSGVS 60
           MGVV+R EETF+SELE+ RR LVAC GV RRK G+EFKSLKPKPIDDA+EKVVCVTSGVS
Sbjct: 1   MGVVVRYEETFVSELERCRRMLVACAGVQRRKGGKEFKSLKPKPIDDADEKVVCVTSGVS 60

Query: 61  FLGSAIVNELSIHGYSVRVIVDNPEDVNKLEETTSGRHNVTVVVAKLTDVNSLVEAFNGC 120
           FLGSAIVNELS+HGYSVRVIVDNPEDVNKLEETTSGRHNVTVVVA LTDVNSLVEAFNGC
Sbjct: 61  FLGSAIVNELSLHGYSVRVIVDNPEDVNKLEETTSGRHNVTVVVADLTDVNSLVEAFNGC 120

Query: 121 RGVFHTSSSIDPAGVSGYTKVMSEVEMRTTENVMEACARTETVKNCVLTSSLLACIWRDQ 180
           RGVFHTSSSIDP+GVSGYTKVMSEVEM+TTENVM+ACARTETV+NCVLTSSLLACIWRDQ
Sbjct: 121 RGVFHTSSSIDPSGVSGYTKVMSEVEMKTTENVMQACARTETVRNCVLTSSLLACIWRDQ 180

Query: 181 TGESESTGASRIVNHGSWSSLQLCQDKKLWFALGKLKSEKVAWKIAQESGLKLATICSAL 240
             ESES G+SRIV+H  WSSLQLCQDKKLWFALGKLKSEKVAWKIAQESGLKLATICSAL
Sbjct: 181 AVESESIGSSRIVDHACWSSLQLCQDKKLWFALGKLKSEKVAWKIAQESGLKLATICSAL 240

Query: 241 VTGPNFHRRNSTPTMAYLKGAREMYTNSLLATVDVTKLAKAHVCVYEGMKENASGRYICF 300
           +TGPNFHRR+ST T+AYLKGAREMYTN LLATVDVTKLAKAHVCVYEGMKEN+S RYICF
Sbjct: 241 LTGPNFHRRSSTATIAYLKGAREMYTNGLLATVDVTKLAKAHVCVYEGMKENSSSRYICF 300

Query: 301 DRVISTVDEATKLASEIGVPVGTIVQTTPPMDSPISFQLSNKKLSDLMSRSNLQRSCLNE 360
           DRVIST +EAT LA+EIGVPVG+IVQTTPP+DSP+SFQLSNKKLSDLM RSNLQRSCLNE
Sbjct: 301 DRVISTPNEATMLAAEIGVPVGSIVQTTPPIDSPVSFQLSNKKLSDLMFRSNLQRSCLNE 360

Query: 361 NRFS 365
           +  S
Sbjct: 361 HTSS 364

BLAST of Spg039203 vs. NCBI nr
Match: XP_022953600.1 (cinnamoyl-CoA reductase-like SNL6 [Cucurbita moschata])

HSP 1 Score: 629.4 bits (1622), Expect = 1.9e-176
Identity = 321/363 (88.43%), Postives = 338/363 (93.11%), Query Frame = 0

Query: 1   MGVVIRSEETFLSELEQLRRKLVACGGVHRRKDGEEFKSLKPKPIDDAEEKVVCVTSGVS 60
           MGV+ RSEET +SELEQLRRKLVACGG+ RRKDGEEFKSLK K IDDAEEKVVCVTSGVS
Sbjct: 1   MGVLFRSEETLVSELEQLRRKLVACGGIQRRKDGEEFKSLKSKLIDDAEEKVVCVTSGVS 60

Query: 61  FLGSAIVNELSIHGYSVRVIVDNPEDVNKLEETTSGRHNVTVVVAKLTDVNSLVEAFNGC 120
           FLGSAIVNEL +HGYSVRVIVDNPEDVNKL+ETTSGR NVTVVVAKLTDVNSLVEAFNGC
Sbjct: 61  FLGSAIVNELLVHGYSVRVIVDNPEDVNKLDETTSGRPNVTVVVAKLTDVNSLVEAFNGC 120

Query: 121 RGVFHTSSSIDPAGVSGYTKVMSEVEMRTTENVMEACARTETVKNCVLTSSLLACIWRDQ 180
           RGVFHTSSSIDPAG+SGYTKVMSEVEMR+TENVMEACARTETV+NCVLTSSLLACIWRDQ
Sbjct: 121 RGVFHTSSSIDPAGLSGYTKVMSEVEMRSTENVMEACARTETVRNCVLTSSLLACIWRDQ 180

Query: 181 TGESESTGASRIVNHGSWSSLQLCQDKKLWFALGKLKSEKVAWKIAQESGLKLATICSAL 240
               ESTG SR+V+HG WSS QLCQDKKLWFALGKLKSEKVAWKIAQESGLKLATICS L
Sbjct: 181 ---PESTGTSRVVDHGCWSSFQLCQDKKLWFALGKLKSEKVAWKIAQESGLKLATICSPL 240

Query: 241 VTGPNFHRRNSTPTMAYLKGAREMYTNSLLATVDVTKLAKAHVCVYEGMKENASGRYICF 300
           +TG NFH RNSTPTMAYLKGAREMY N LLATVDVTKLAKAHVC+YE +KEN SGRYICF
Sbjct: 241 ITGSNFHSRNSTPTMAYLKGAREMYKNGLLATVDVTKLAKAHVCIYEELKENGSGRYICF 300

Query: 301 DRVISTVDEATKLASEIGVPVGTIVQTTPPMDSPISFQLSNKKLSDLMSRSNLQRSCLNE 360
           DRVISTVDEATKLA+EI VPVGTIV+TT P+DSPI FQLSNKK+SDLMSRSNL RSCLNE
Sbjct: 301 DRVISTVDEATKLAAEIRVPVGTIVETTLPIDSPIPFQLSNKKVSDLMSRSNLGRSCLNE 360

Query: 361 NRF 364
           NRF
Sbjct: 361 NRF 360

BLAST of Spg039203 vs. ExPASy Swiss-Prot
Match: Q0JKZ0 (Cinnamoyl-CoA reductase-like SNL6 OS=Oryza sativa subsp. japonica OX=39947 GN=SNL6 PE=3 SV=1)

HSP 1 Score: 286.2 bits (731), Expect = 5.2e-76
Identity = 162/378 (42.86%), Postives = 221/378 (58.47%), Query Frame = 0

Query: 4   VIRSEETFLSELEQLRRKLV----------ACGGVHRRKDGEEFKSLKPKPIDDAEEKVV 63
           V+RS ++  +E+E++R  L+          A  G        + K         A  + V
Sbjct: 3   VLRSTQSMQAEVEEMRAALLHGHGGGAAAAAAAGWRPSAGDADVKRTAGGDGGAAGPRTV 62

Query: 64  CVTSGVSFLGSAIVNELSIHGYSVRVIVDNPEDVNKLEET----TSGRHNVTVVVAKLTD 123
           CVT G+SF+G A+V+ L  HGY+VR+ ++  ED++KL E       GR  V  V+A +TD
Sbjct: 63  CVTGGISFVGFAVVDRLLRHGYTVRLALETQEDLDKLREMEMFGEDGRDGVWTVMANVTD 122

Query: 124 VNSLVEAFNGCRGVFHTSSSIDPAGVSGYTKVMSEVEMRTTENVMEACARTETVKNCVLT 183
             SL  AF+GC GVFHTS+ +DP G+SGYTK M+ +E +  E V+EAC RTE+V+ CV T
Sbjct: 123 PESLHRAFDGCAGVFHTSAFVDPGGMSGYTKHMASLEAKAAEQVIEACVRTESVRKCVFT 182

Query: 184 SSLLACIWRDQTGESESTGASRIVNHGSWSSLQLCQDKKLWFALGKLKSEKVAWKIAQES 243
           SSLLAC+WR             I++   WS    C+D KLWFALGK  +EK AW+ A+  
Sbjct: 183 SSLLACVWRQNYPHDRR--FPTIIDENCWSDESFCRDNKLWFALGKTAAEKTAWRAARGR 242

Query: 244 GLKLATICSALVTGPNFHRRNSTPTMAYLKGAREMYTNSLLATVDVTKLAKAHVCVYEGM 303
            LKL T+C ALVTGP F RRNST ++AYLKGAR M  + LLAT  V  +A+AHV VYE M
Sbjct: 243 DLKLVTVCPALVTGPGFRRRNSTASIAYLKGARAMLADGLLATASVETVAEAHVRVYEAM 302

Query: 304 KEN-ASGRYICFDRVISTVDEATKLASEIGVP--VGTIVQTTPPMDSPISFQLSNKKLSD 363
            +N A GRYIC+D V+   +E  +L  ++G+P             D P  F L  +KL+ 
Sbjct: 303 GDNTAGGRYICYDHVVKRPEEFAELERQLGIPRRAAAAAAAQDSGDRPARFDLCRQKLAR 362

Query: 364 LMSRSNLQRSCLNENRFS 365
           LMS    +R C  ++ +S
Sbjct: 363 LMS---TRRRCTYDDYYS 375

BLAST of Spg039203 vs. ExPASy Swiss-Prot
Match: A0A059TC02 (Cinnamoyl-CoA reductase 1 OS=Petunia hybrida OX=4102 GN=CCR1 PE=1 SV=1)

HSP 1 Score: 145.6 bits (366), Expect = 1.1e-33
Identity = 111/320 (34.69%), Postives = 158/320 (49.38%), Query Frame = 0

Query: 51  KVVCVTSGVSFLGSAIVNELSIHGYSVRVIVDNPEDVNK--LEETTSGRHNVTVVVAKLT 110
           +VVCVT    F+ S +V  L   GY+VR  V NP+D     L E    +  +T+  A L 
Sbjct: 7   QVVCVTGAGGFIASWLVKILLEKGYTVRGTVRNPDDPKNGHLRELEGAKERLTLCKADLL 66

Query: 111 DVNSLVEAFNGCRGVFHTSSSI--DPAGVSGYTKVMSEVEMRTTENVMEACARTETVKNC 170
           D  SL EA NGC GVFHT+S +  DP       + M E  +  T+NV+ A A    V+  
Sbjct: 67  DYQSLREAINGCDGVFHTASPVTDDP-------EQMVEPAVIGTKNVINAAAEA-NVRRV 126

Query: 171 VLTSSLLACIWRDQTGESESTGASRIVNHGSWSSLQLCQDKKLWFALGKLKSEKVAWKIA 230
           V TSS +  ++ D   + E+     +V+   WS    C++ K W+  GK+ +E+ AW+ A
Sbjct: 127 VFTSS-IGAVYMDPNRDPET-----VVDETCWSDPDFCKNTKNWYCYGKMVAEQAAWEEA 186

Query: 231 QESGLKLATICSALVTGPNFHRRNSTPT---MAYLKGAREMYTNSLLATVDVTKLAKAHV 290
           +E G+ L  I   LV GP      +      + YL G+ + Y NS+ A VDV  +A AH+
Sbjct: 187 KEKGVDLVVINPVLVQGPLLQTTVNASVLHILKYLTGSAKTYANSVQAYVDVKDVALAHI 246

Query: 291 CVYEGMKENASGRYICFDRVI---STVDEATKLASEIGVPVGTIVQTTPPMDSPISFQLS 350
            +YE     ASGRY+C + V+     V+  +K   E  +P       T P   P  F  S
Sbjct: 247 LLYE--TPEASGRYLCAESVLHRGDVVEILSKFFPEYPIPT-KCSDVTKPRVKPYKF--S 306

Query: 351 NKKLSDLMSRSNLQRSCLNE 361
           N+KL DL       + CL E
Sbjct: 307 NQKLKDLGLEFTPVKQCLYE 307

BLAST of Spg039203 vs. ExPASy Swiss-Prot
Match: Q500U8 (Tetraketide alpha-pyrone reductase 1 OS=Arabidopsis thaliana OX=3702 GN=TKPR1 PE=1 SV=1)

HSP 1 Score: 117.5 bits (293), Expect = 3.2e-25
Identity = 81/268 (30.22%), Postives = 135/268 (50.37%), Query Frame = 0

Query: 45  IDDAEEKVVCVTSGVSFLGSAIVNELSIHGYSVRVIVDNPEDVNKLE---ETTSGRHNVT 104
           +D A+ K VCVT    FL S +V  L + GY V   V +P +  KL    +    +  + 
Sbjct: 1   MDQAKGK-VCVTGASGFLASWLVKRLLLEGYEVIGTVRDPGNEKKLAHLWKLEGAKERLR 60

Query: 105 VVVAKLTDVNSLVEAFNGCRGVFHTSSSIDPAGVSGYTKVMSEVEMRTTENVMEACARTE 164
           +V A L +  S   A  GC+GVFHT+S +     +   +++    +  T NV+ +C +  
Sbjct: 61  LVKADLMEEGSFDNAIMGCQGVFHTASPVLKPTSNPEEEILRPA-IEGTLNVLRSCRKNP 120

Query: 165 TVKNCVLTSSLLACIWRDQTGESESTGASRIVNHGSWSSLQLCQDKKLWFALGKLKSEKV 224
           ++K  VLTSS      RD             ++   W+S++LC+  ++W+AL K  +E+ 
Sbjct: 121 SLKRVVLTSSSSTVRIRDDFDPKIP------LDESIWTSVELCKRFQVWYALSKTLAEQA 180

Query: 225 AWKIAQESGLKLATICSALVTGPNFHR---RNSTPTMAYLKGAREMYT-NSLLATVDVTK 284
           AWK ++E+G+ L T+  + + GP+        ++  +  LKG  E +  +  +  V +  
Sbjct: 181 AWKFSEENGIDLVTVLPSFLVGPSLPPDLCSTASDVLGLLKGETEKFQWHGQMGYVHIDD 240

Query: 285 LAKAHVCVYEGMKENASGRYICFDRVIS 306
           +A+ H+ V+E   E A GRYIC   VIS
Sbjct: 241 VARTHIVVFE--HEAAQGRYICSSNVIS 258

BLAST of Spg039203 vs. ExPASy Swiss-Prot
Match: Q9CA28 (Tetraketide alpha-pyrone reductase 2 OS=Arabidopsis thaliana OX=3702 GN=TKPR2 PE=1 SV=1)

HSP 1 Score: 115.2 bits (287), Expect = 1.6e-24
Identity = 83/256 (32.42%), Postives = 122/256 (47.66%), Query Frame = 0

Query: 55  VTSGVSFLGSAIVNELSIHGYSVRVIVDNPEDVNK---LEETTSGRHNVTVVVAKLTDVN 114
           VT G  F+ S I+  L   G++VR  V NP D  K   L E    +  + ++ A LT   
Sbjct: 6   VTGGTGFIASYIIKSLLELGHTVRTTVRNPRDEEKVGFLWEFQGAKQRLKILQADLTVEG 65

Query: 115 SLVEAFNGCRGVFHTSSSIDPAGVSGYTKVMSEVEMRTTENVMEACARTE-TVKNCVLTS 174
           S  EA NG  GVFHT+S +         + + +  ++ T NVM +CA+++ T+K  VLTS
Sbjct: 66  SFDEAVNGVDGVFHTASPVLVPQDHNIQETLVDPIIKGTTNVMSSCAKSKATLKRIVLTS 125

Query: 175 SLLACIWRDQTGESESTGASRIVNHGSWSSLQLCQDKKLWFALGKLKSEKVAWKIAQESG 234
           S  +  +R    E+        +N   WS  + C+   LW+   K   E+ AW+IA+E G
Sbjct: 126 SCSSIRYRFDATEASP------LNESHWSDPEYCKRFNLWYGYAKTLGEREAWRIAEEKG 185

Query: 235 LKLATICSALVTGPNFHRRNSTP---TMAYLKGAREMYTNSLLATVDVTKLAKAHVCVYE 294
           L L  +  + V GP    + ++     +A  KG    Y N  +  V +  +  AHV   E
Sbjct: 186 LDLVVVNPSFVVGPLLGPKPTSTLLMILAIAKGLAGEYPNFTVGFVHIDDVVAAHVLAME 245

Query: 295 GMKENASGRYICFDRV 304
             K  ASGR IC   V
Sbjct: 246 EPK--ASGRIICSSSV 253

BLAST of Spg039203 vs. ExPASy Swiss-Prot
Match: Q6K9A2 (Cinnamoyl-CoA reductase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=CCR1 PE=1 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 2.7e-24
Identity = 97/325 (29.85%), Postives = 150/325 (46.15%), Query Frame = 0

Query: 46  DDAEEKVVCVTSGVSFLGSAIVNELSIHGYSVRVIVDNPEDVNK--LEETTSGRHNVTVV 105
           ++ E+++VCVT    F+GS +V EL I GY VR    +P D     L E       +++ 
Sbjct: 11  NNGEKQLVCVTGAGGFIGSWVVKELLIRGYHVRGTARDPADSKNAHLLELEGADERLSLC 70

Query: 106 VAKLTDVNSLVEAFNGCRGVFHTSSSI--DPAGVSGYTKVMSEVEMRTTENVMEACARTE 165
            A + D  SL  AF+GC GVFH +S +  DP         +  V +  T NV+ A A   
Sbjct: 71  RADVLDAASLRAAFSGCHGVFHVASPVSNDPD--------LVPVAVEGTRNVINAAA-DM 130

Query: 166 TVKNCVLTSSLLACIWRDQTGESESTGASRIVNHGSWSSLQLCQDKKLWFALGKLKSEKV 225
            V+  V TSS  A         + +     +++   WS  + C+     +   K+ +E  
Sbjct: 131 GVRRVVFTSSYGA------VHMNPNRSPDAVLDETCWSDYEFCKQTDNLYCCAKMMAEMT 190

Query: 226 AWKIAQESGLKLATICSALVTGPNFHRR---NSTPTMAYLKGAREMYTNSLLATVDVTKL 285
           A + A + GL+LA +  ++  GP   +    ++     YL G ++ Y N++ A VDV  +
Sbjct: 191 ATEEAAKRGLELAVVVPSMTMGPMLQQTLNFSTNHVARYLMGTKKSYPNAVAAYVDVRDV 250

Query: 286 AKAHVCVYEGMKENASGRYICFDRVISTVDEATKLASEIGVPVGTIVQTT---PPMDSPI 345
           A+AHV VYE  +  A GRY+C   V+    E  ++  E+        +      PM  P 
Sbjct: 251 ARAHVLVYE--RPEARGRYLCIGTVLHRA-ELLRMLRELFPRYPATAKCEDDGKPMAKPY 310

Query: 346 SFQLSNKKLSDLMSRSNLQRSCLNE 361
            F  SN++L DL       R  LNE
Sbjct: 311 KF--SNQRLKDLGLEFTPLRKSLNE 315

BLAST of Spg039203 vs. ExPASy TrEMBL
Match: A0A6J1FEE8 (cinnamoyl-CoA reductase-like SNL6 OS=Cucurbita moschata OX=3662 GN=LOC111444756 PE=3 SV=1)

HSP 1 Score: 640.6 bits (1651), Expect = 4.0e-180
Identity = 321/360 (89.17%), Postives = 343/360 (95.28%), Query Frame = 0

Query: 1   MGVVIRSEETFLSELEQLRRKLVACGGVHRRKDGEEFKSLKPKPIDDAEEKVVCVTSGVS 60
           MGVV+RSEETF+SELE+ RR LVAC GV RRKDG+EFKSLKP+PIDDA+EKVVCVTSGVS
Sbjct: 1   MGVVVRSEETFVSELERCRRMLVACAGVQRRKDGKEFKSLKPRPIDDADEKVVCVTSGVS 60

Query: 61  FLGSAIVNELSIHGYSVRVIVDNPEDVNKLEETTSGRHNVTVVVAKLTDVNSLVEAFNGC 120
           FLGSAIVNELS+HGY VRVIVDNPEDVNKLEETTSGRHNVTVVVA LTDVNSLVEAFNGC
Sbjct: 61  FLGSAIVNELSLHGYFVRVIVDNPEDVNKLEETTSGRHNVTVVVADLTDVNSLVEAFNGC 120

Query: 121 RGVFHTSSSIDPAGVSGYTKVMSEVEMRTTENVMEACARTETVKNCVLTSSLLACIWRDQ 180
           RGVFHTSSSIDP+GVSGYTKVMSEVEMRTTENVMEACARTETV+NCVLTSSLLACIWRDQ
Sbjct: 121 RGVFHTSSSIDPSGVSGYTKVMSEVEMRTTENVMEACARTETVRNCVLTSSLLACIWRDQ 180

Query: 181 TGESESTGASRIVNHGSWSSLQLCQDKKLWFALGKLKSEKVAWKIAQESGLKLATICSAL 240
            GESES G+SRIV+H  WSSLQLCQDKKLWFALGKLKSEKVAWKIAQESGLKLATICSAL
Sbjct: 181 AGESESIGSSRIVDHACWSSLQLCQDKKLWFALGKLKSEKVAWKIAQESGLKLATICSAL 240

Query: 241 VTGPNFHRRNSTPTMAYLKGAREMYTNSLLATVDVTKLAKAHVCVYEGMKENASGRYICF 300
           +TGPNFHRR+ST T+AYLKGAREMYTN LLATVDVTKLAKAHVCVYEGMKEN+S RYICF
Sbjct: 241 ITGPNFHRRSSTATIAYLKGAREMYTNGLLATVDVTKLAKAHVCVYEGMKENSSSRYICF 300

Query: 301 DRVISTVDEATKLASEIGVPVGTIVQTTPPMDSPISFQLSNKKLSDLMSRSNLQRSCLNE 360
           DRVIST +EA+ LA+EIGVPVG+IVQTTPP++SP+SFQLSNKKL DLM RSNLQRSCLNE
Sbjct: 301 DRVISTANEASMLATEIGVPVGSIVQTTPPINSPVSFQLSNKKLCDLMFRSNLQRSCLNE 360

BLAST of Spg039203 vs. ExPASy TrEMBL
Match: A0A6J1K2V0 (cinnamoyl-CoA reductase-like SNL6 OS=Cucurbita maxima OX=3661 GN=LOC111489565 PE=3 SV=1)

HSP 1 Score: 637.1 bits (1642), Expect = 4.5e-179
Identity = 322/364 (88.46%), Postives = 344/364 (94.51%), Query Frame = 0

Query: 1   MGVVIRSEETFLSELEQLRRKLVACGGVHRRKDGEEFKSLKPKPIDDAEEKVVCVTSGVS 60
           MGVV+R EETF+SELE+ RR LVAC GV RRK G+EFKSLKPKPIDDA+EKVVCVTSGVS
Sbjct: 1   MGVVVRYEETFVSELERCRRMLVACAGVQRRKGGKEFKSLKPKPIDDADEKVVCVTSGVS 60

Query: 61  FLGSAIVNELSIHGYSVRVIVDNPEDVNKLEETTSGRHNVTVVVAKLTDVNSLVEAFNGC 120
           FLGSAIVNELS+HGYSVRVIVDNPEDVNKLEETTSGRHNVTVVVA LTDVNSLVEAFNGC
Sbjct: 61  FLGSAIVNELSLHGYSVRVIVDNPEDVNKLEETTSGRHNVTVVVADLTDVNSLVEAFNGC 120

Query: 121 RGVFHTSSSIDPAGVSGYTKVMSEVEMRTTENVMEACARTETVKNCVLTSSLLACIWRDQ 180
           RGVFHTSSSIDP+GVSGYTKVMSEVEM+TTENVM+ACARTETV+NCVLTSSLLACIWRDQ
Sbjct: 121 RGVFHTSSSIDPSGVSGYTKVMSEVEMKTTENVMQACARTETVRNCVLTSSLLACIWRDQ 180

Query: 181 TGESESTGASRIVNHGSWSSLQLCQDKKLWFALGKLKSEKVAWKIAQESGLKLATICSAL 240
             ESES G+SRIV+H  WSSLQLCQDKKLWFALGKLKSEKVAWKIAQESGLKLATICSAL
Sbjct: 181 AVESESIGSSRIVDHACWSSLQLCQDKKLWFALGKLKSEKVAWKIAQESGLKLATICSAL 240

Query: 241 VTGPNFHRRNSTPTMAYLKGAREMYTNSLLATVDVTKLAKAHVCVYEGMKENASGRYICF 300
           +TGPNFHRR+ST T+AYLKGAREMYTN LLATVDVTKLAKAHVCVYEGMKEN+S RYICF
Sbjct: 241 LTGPNFHRRSSTATIAYLKGAREMYTNGLLATVDVTKLAKAHVCVYEGMKENSSSRYICF 300

Query: 301 DRVISTVDEATKLASEIGVPVGTIVQTTPPMDSPISFQLSNKKLSDLMSRSNLQRSCLNE 360
           DRVIST +EAT LA+EIGVPVG+IVQTTPP+DSP+SFQLSNKKLSDLM RSNLQRSCLNE
Sbjct: 301 DRVISTPNEATMLAAEIGVPVGSIVQTTPPIDSPVSFQLSNKKLSDLMFRSNLQRSCLNE 360

Query: 361 NRFS 365
           +  S
Sbjct: 361 HTSS 364

BLAST of Spg039203 vs. ExPASy TrEMBL
Match: A0A6J1GQ53 (cinnamoyl-CoA reductase-like SNL6 OS=Cucurbita moschata OX=3662 GN=LOC111456087 PE=3 SV=1)

HSP 1 Score: 629.4 bits (1622), Expect = 9.3e-177
Identity = 321/363 (88.43%), Postives = 338/363 (93.11%), Query Frame = 0

Query: 1   MGVVIRSEETFLSELEQLRRKLVACGGVHRRKDGEEFKSLKPKPIDDAEEKVVCVTSGVS 60
           MGV+ RSEET +SELEQLRRKLVACGG+ RRKDGEEFKSLK K IDDAEEKVVCVTSGVS
Sbjct: 1   MGVLFRSEETLVSELEQLRRKLVACGGIQRRKDGEEFKSLKSKLIDDAEEKVVCVTSGVS 60

Query: 61  FLGSAIVNELSIHGYSVRVIVDNPEDVNKLEETTSGRHNVTVVVAKLTDVNSLVEAFNGC 120
           FLGSAIVNEL +HGYSVRVIVDNPEDVNKL+ETTSGR NVTVVVAKLTDVNSLVEAFNGC
Sbjct: 61  FLGSAIVNELLVHGYSVRVIVDNPEDVNKLDETTSGRPNVTVVVAKLTDVNSLVEAFNGC 120

Query: 121 RGVFHTSSSIDPAGVSGYTKVMSEVEMRTTENVMEACARTETVKNCVLTSSLLACIWRDQ 180
           RGVFHTSSSIDPAG+SGYTKVMSEVEMR+TENVMEACARTETV+NCVLTSSLLACIWRDQ
Sbjct: 121 RGVFHTSSSIDPAGLSGYTKVMSEVEMRSTENVMEACARTETVRNCVLTSSLLACIWRDQ 180

Query: 181 TGESESTGASRIVNHGSWSSLQLCQDKKLWFALGKLKSEKVAWKIAQESGLKLATICSAL 240
               ESTG SR+V+HG WSS QLCQDKKLWFALGKLKSEKVAWKIAQESGLKLATICS L
Sbjct: 181 ---PESTGTSRVVDHGCWSSFQLCQDKKLWFALGKLKSEKVAWKIAQESGLKLATICSPL 240

Query: 241 VTGPNFHRRNSTPTMAYLKGAREMYTNSLLATVDVTKLAKAHVCVYEGMKENASGRYICF 300
           +TG NFH RNSTPTMAYLKGAREMY N LLATVDVTKLAKAHVC+YE +KEN SGRYICF
Sbjct: 241 ITGSNFHSRNSTPTMAYLKGAREMYKNGLLATVDVTKLAKAHVCIYEELKENGSGRYICF 300

Query: 301 DRVISTVDEATKLASEIGVPVGTIVQTTPPMDSPISFQLSNKKLSDLMSRSNLQRSCLNE 360
           DRVISTVDEATKLA+EI VPVGTIV+TT P+DSPI FQLSNKK+SDLMSRSNL RSCLNE
Sbjct: 301 DRVISTVDEATKLAAEIRVPVGTIVETTLPIDSPIPFQLSNKKVSDLMSRSNLGRSCLNE 360

Query: 361 NRF 364
           NRF
Sbjct: 361 NRF 360

BLAST of Spg039203 vs. ExPASy TrEMBL
Match: A0A6J1JPH8 (cinnamoyl-CoA reductase-like SNL6 OS=Cucurbita maxima OX=3661 GN=LOC111487721 PE=3 SV=1)

HSP 1 Score: 623.2 bits (1606), Expect = 6.7e-175
Identity = 319/363 (87.88%), Postives = 336/363 (92.56%), Query Frame = 0

Query: 1   MGVVIRSEETFLSELEQLRRKLVACGGVHRRKDGEEFKSLKPKPIDDAEEKVVCVTSGVS 60
           MGV+ RSEET +SELEQLRRKLVACGG+ RRKDGEEF+SLK K IDDAEEKVVCVTSGVS
Sbjct: 1   MGVLFRSEETLVSELEQLRRKLVACGGIQRRKDGEEFRSLKSKLIDDAEEKVVCVTSGVS 60

Query: 61  FLGSAIVNELSIHGYSVRVIVDNPEDVNKLEETTSGRHNVTVVVAKLTDVNSLVEAFNGC 120
           FLGSAIVNEL +HGYSVRVIVDNPEDVNKL+ETTSGR NVTVVVAKLTDVNSLVEAFNGC
Sbjct: 61  FLGSAIVNELLVHGYSVRVIVDNPEDVNKLDETTSGRPNVTVVVAKLTDVNSLVEAFNGC 120

Query: 121 RGVFHTSSSIDPAGVSGYTKVMSEVEMRTTENVMEACARTETVKNCVLTSSLLACIWRDQ 180
           RGVFHTSSSIDPAG+SGYTKVMSEVEMR+TENVMEACARTETV+NCVLTSSLLACIWRDQ
Sbjct: 121 RGVFHTSSSIDPAGLSGYTKVMSEVEMRSTENVMEACARTETVRNCVLTSSLLACIWRDQ 180

Query: 181 TGESESTGASRIVNHGSWSSLQLCQDKKLWFALGKLKSEKVAWKIAQESGLKLATICSAL 240
               ESTG SR+V+HG WSS QLCQDKKLWFALGKLKSEKVAWKIAQESGLKLATICS L
Sbjct: 181 ---PESTGTSRVVDHGCWSSFQLCQDKKLWFALGKLKSEKVAWKIAQESGLKLATICSPL 240

Query: 241 VTGPNFHRRNSTPTMAYLKGAREMYTNSLLATVDVTKLAKAHVCVYEGMKENASGRYICF 300
           +TG NFH RNSTPTMAYLKGAREMY N LLATVDVTKLAKAHVCVYE +KEN SGRYICF
Sbjct: 241 ITGSNFHSRNSTPTMAYLKGAREMYKNGLLATVDVTKLAKAHVCVYEELKENGSGRYICF 300

Query: 301 DRVISTVDEATKLASEIGVPVGTIVQTTPPMDSPISFQLSNKKLSDLMSRSNLQRSCLNE 360
           DRVISTVDEATKLA EI VPV TIV+TT P+DSPI FQLSNKK+SDLMSRSN+ RSCLNE
Sbjct: 301 DRVISTVDEATKLAVEIKVPVETIVETTLPIDSPIPFQLSNKKVSDLMSRSNVGRSCLNE 360

Query: 361 NRF 364
           NRF
Sbjct: 361 NRF 360

BLAST of Spg039203 vs. ExPASy TrEMBL
Match: A0A6J1BZP5 (cinnamoyl-CoA reductase-like SNL6 OS=Momordica charantia OX=3673 GN=LOC111006177 PE=3 SV=1)

HSP 1 Score: 597.4 bits (1539), Expect = 3.9e-167
Identity = 297/363 (81.82%), Postives = 333/363 (91.74%), Query Frame = 0

Query: 1   MGVVIRSEETFLSELEQLRRKLVACGGVHRRKDGEEFKSLKPKPIDDAEEKVVCVTSGVS 60
           MGVV R+EET + ELEQLRRKLVACGG+ RRKDG+E+++LK KPIDDAEEK+VCVTSG S
Sbjct: 1   MGVVFRAEETLVLELEQLRRKLVACGGIQRRKDGDEYRNLKSKPIDDAEEKIVCVTSGAS 60

Query: 61  FLGSAIVNELSIHGYSVRVIVDNPEDVNKLEETTSGRHNVTVVVAKLTDVNSLVEAFNGC 120
           FLG+A+VNEL +HGYSVRVIVDNPEDV+KLEET S RH+VTVV AKLTDV SLVEAF+GC
Sbjct: 61  FLGAALVNELLLHGYSVRVIVDNPEDVSKLEETMSNRHDVTVVAAKLTDVESLVEAFDGC 120

Query: 121 RGVFHTSSSIDPAGVSGYTKVMSEVEMRTTENVMEACARTETVKNCVLTSSLLACIWRDQ 180
           RGVFHTSSSIDP+G+SGYTK+MSEVEM+TTENVMEAC RTETV+NCVLTSSLLACIW+DQ
Sbjct: 121 RGVFHTSSSIDPSGLSGYTKIMSEVEMKTTENVMEACTRTETVRNCVLTSSLLACIWQDQ 180

Query: 181 TGESESTGASRIVNHGSWSSLQLCQDKKLWFALGKLKSEKVAWKIAQESGLKLATICSAL 240
            GESES   SR+V+HG WSSLQLCQDKKLW+ALGKLKSEKVAW++AQESGLKLATICSAL
Sbjct: 181 AGESESE-TSRVVDHGCWSSLQLCQDKKLWYALGKLKSEKVAWRMAQESGLKLATICSAL 240

Query: 241 VTGPNFHRRNSTPTMAYLKGAREMYTNSLLATVDVTKLAKAHVCVYEGMKENASGRYICF 300
           +TGPNFHRRNSTPTMAYLKGAREMY N +LATV+VTKLAKAHVCVYEGMKENASGRYICF
Sbjct: 241 ITGPNFHRRNSTPTMAYLKGAREMYANGVLATVEVTKLAKAHVCVYEGMKENASGRYICF 300

Query: 301 DRVISTVDEATKLASEIGVPVGTIVQTTPPMDSPISFQLSNKKLSDLMSRSNLQRSCLNE 360
           DRVISTV EA KLA+E+ VPV T++QTTPP  S I FQLSNKKLS LMSR++L R+CLNE
Sbjct: 301 DRVISTVGEAEKLAAEMAVPVTTMLQTTPPTHSSIPFQLSNKKLSHLMSRTDLPRTCLNE 360

Query: 361 NRF 364
           + F
Sbjct: 361 SIF 362

BLAST of Spg039203 vs. TAIR 10
Match: AT5G14700.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 350.9 bits (899), Expect = 1.2e-96
Identity = 194/377 (51.46%), Postives = 258/377 (68.44%), Query Frame = 0

Query: 4   VIRSEETFLSELEQLRRKLVACGGVHRRKDGEEF-------KSLKPKPID-DAEEKVVCV 63
           ++R+ ETF +EL++       C  V RRKD + F       KS     +D DA  ++VCV
Sbjct: 3   IVRATETFSAELKEF-----MCAAVQRRKDDDGFRGSRGGGKSRNAMDLDSDAGNRLVCV 62

Query: 64  TSGVSFLGSAIVNELSIHGYSVRVIVDNPEDVNKLE------ETTSGRHNVTVVVAKLTD 123
           T GVS+LG AIV  L +HGYSVR++VD PED  K+       ET S  + +T VV++LT+
Sbjct: 63  TGGVSYLGRAIVKRLLVHGYSVRIVVDCPEDKEKVSEMEADAETASFSNMITSVVSRLTE 122

Query: 124 VNSLVEAFNGCRGVFHTSSSIDPAGVSGYTKVMSEVEMRTTENVMEACARTETVKNCVLT 183
           ++SL++AF+GC GVFHTS+ +DPAGVSGY+K M+E+E + +E+V+EAC RT +V+ CV T
Sbjct: 123 IDSLIKAFDGCAGVFHTSAFVDPAGVSGYSKSMAELEAKVSESVIEACTRTASVRKCVFT 182

Query: 184 SSLLACIWRDQTGESESTGASRIVNHGSWSSLQLCQDKKLWFALGKLKSEKVAWKIAQES 243
           SSLLAC W+     S       ++N  SWS  QLC D KLW+ALGKLK+EK AW+IA   
Sbjct: 183 SSLLACAWQKNPCNSLD---HSVINEESWSDEQLCIDNKLWYALGKLKAEKAAWRIADSK 242

Query: 244 GLKLATICSALVTGPNFHRRNSTPTMAYLKGAREMYTNSLLATVDVTKLAKAHVCVYEGM 303
           GLKLATIC AL+TGP+F  RNST T+AYLKGA+EMY+N LLAT+DV +LAKAHVC++EG+
Sbjct: 243 GLKLATICPALITGPDFFNRNSTSTLAYLKGAKEMYSNGLLATMDVNRLAKAHVCLWEGL 302

Query: 304 -KENASGRYICFDRVISTVDEATKLASEIGVPVGTIVQTTPPMD----SPISFQLSNKKL 362
             + A GRYICFD ++S  D A KLA +I V +  I   +   D    +  S Q+S+KKL
Sbjct: 303 GNKTAFGRYICFDTILSR-DGAEKLAKDIDVQIEKICGNSNDSDANTETEASLQISDKKL 362

BLAST of Spg039203 vs. TAIR 10
Match: AT2G23910.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 165.2 bits (417), Expect = 9.6e-41
Identity = 105/308 (34.09%), Postives = 163/308 (52.92%), Query Frame = 0

Query: 49  EEKVV---CVTSGVSFLGSAIVNELSIHGYSVRVIV-DNPEDV--NKLEETTSGRHNVTV 108
           +EK +   CV    +++G  I+  L   GYSV   +  N E V   K+ +  +    + V
Sbjct: 3   QEKTISCCCVLDASTYVGFWILKRLLTRGYSVHAAIRKNGESVLEEKIRDMEANEERLEV 62

Query: 109 VVAKLTDVNSLVEAFNGCRGVFHTSSSIDPAGVSGYTKVMSEVEMRTTENVMEACARTET 168
               + D  S++ + N C  VF      +P G     +V  ++E+R   NV+EACARTE+
Sbjct: 63  YDVDVLDYQSILISLNNCNAVFCCLD--NPEGYDEEKEV--DLEVRGAINVVEACARTES 122

Query: 169 VKNCVLTSSLLACIWRDQTGESESTGASRIVNHGSWSSLQLCQDKKLWFALGKLKSEKVA 228
           ++  V +SSL A IWRD      + G  + V+   WS L  C  KKLW AL K +SEK A
Sbjct: 123 IEKIVFSSSLTAAIWRD------NIGTQKDVDEKCWSDLDFCLKKKLWHALAKTQSEKAA 182

Query: 229 WKIAQESGLKLATICSALVTGPNFHRRNSTPTMAYLKGAREMYTNSLLATVDVTKLAKAH 288
           W +A +  + + ++   L+ GP+  + N  PTM+YLKGA +MY N +LA VDV  +A  H
Sbjct: 183 WALAMDRMVNMVSVNPGLIVGPSVAQHNPRPTMSYLKGAAQMYENGVLAYVDVEFVADVH 242

Query: 289 VCVYEGMKENASGRYICFDRVISTVDEATKLASEIG--VPVGTIVQTTPPMDSPISFQLS 348
           +  +E    +A GRY CF+++++T +EA KL   +   +P+    +           +L 
Sbjct: 243 IRAFE--DTSACGRYFCFNQIVNTEEEALKLVQTLSPLIPMPPRHEKEMQGSEVYEERLR 298

BLAST of Spg039203 vs. TAIR 10
Match: AT4G30470.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 165.2 bits (417), Expect = 9.6e-41
Identity = 106/309 (34.30%), Postives = 161/309 (52.10%), Query Frame = 0

Query: 47  DAEEKVVCVTSGVSFLGSAIVNELSIHGYSVRVIV-----DNPEDVNKLEETTSGRHNVT 106
           D      CV    +++G  I+ +L   GYSV   +        E++ +  ETT  R  + 
Sbjct: 4   DKSTSCCCVLDASTYVGFWILKKLLSRGYSVHAAIRRNGESEIEEMIREMETTEER--LV 63

Query: 107 VVVAKLTDVNSLVEAFNGCRGVFHTSSSIDPAGVSGYTKVMSEVEMRTTENVMEACARTE 166
           V    + D  S++ +   C  VF    S +     GY +   ++E+R   NV+EAC RTE
Sbjct: 64  VYDVDVLDYQSILVSLKTCNVVFCCLDSPE-----GYDEKEVDLEVRGAINVVEACGRTE 123

Query: 167 TVKNCVLTSSLLACIWRDQTGESESTGASRIVNHGSWSSLQLCQDKKLWFALGKLKSEKV 226
           +++  V +SSL A IWRD      + G  + V+   WS    C+ KKLW AL K+ SEK 
Sbjct: 124 SIEKIVFSSSLTASIWRD------NIGTQKDVDEKCWSDQDFCRSKKLWHALAKMLSEKA 183

Query: 227 AWKIAQESGLKLATICSALVTGPNFHRRNSTPTMAYLKGAREMYTNSLLATVDVTKLAKA 286
           AW +A +  L + +I   LV GP+  + N+ PTM+YLKGA +MY N +LA VDV  LA  
Sbjct: 184 AWALAMDRRLNMVSINPGLVVGPSVAQHNARPTMSYLKGAAQMYENGVLAYVDVKFLADV 243

Query: 287 HVCVYEGMKENASGRYICFDRVISTVDEATKLASEIG--VPVGTIVQTTPPMDSPISFQL 346
           H+  +E +  +A GRY CF+++++T +EA KL   +   +P+    +           +L
Sbjct: 244 HIRAFEDV--SACGRYFCFNQIVNTEEEALKLVESLSPLIPMPPRYENEMHGSEVYEERL 297

Query: 347 SNKKLSDLM 349
            N KLS L+
Sbjct: 304 RNNKLSKLV 297

BLAST of Spg039203 vs. TAIR 10
Match: AT1G09510.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 126.3 bits (316), Expect = 4.9e-29
Identity = 85/271 (31.37%), Postives = 138/271 (50.92%), Query Frame = 0

Query: 51  KVVCVTSGVSFLGSAIVNELSIHGYSVRVIVDNPEDVNKLEETTS---GRHNVTVVVAKL 110
           K+VCVT    ++ S IV  L + GY+VR  V +P D  K E   +    +  + +  A L
Sbjct: 6   KMVCVTGASGYVASWIVKLLLLRGYTVRATVRDPSDEKKTEHLLALDGAKEKLKLFKADL 65

Query: 111 TDVNSLVEAFNGCRGVFHTSSSIDPAGVSGYTKVMSEVEMRTTENVMEACARTETVKNCV 170
            +  S  +A  GC  VFHT+S +         +++ +  ++ T NV++ CA+  +VK  +
Sbjct: 66  LEEGSFEQAIEGCDAVFHTASPVSLTVTDPQIELI-DPAVKGTLNVLKTCAKVSSVKRVI 125

Query: 171 LTSSLLACIWRDQTGESESTGASRIVNHGSWSSLQLCQDKKLWFALGKLKSEKVAWKIAQ 230
           +TSS+ A ++R+ T      G + +V+   +S    C +KKLW+AL K  +E  AW+ A+
Sbjct: 126 VTSSMAAVLFREPT-----LGPNDLVDESCFSDPNFCTEKKLWYALSKTLAEDEAWRFAK 185

Query: 231 ESGLKLATICSALVTGPNF--HRRNSTPTMAYLKGAREMYTNSLLATVDVTKLAKAHVCV 290
           E GL L  I   LV GP        S   +  L   ++ + N     VDV  +A AH+  
Sbjct: 186 EKGLDLVVINPGLVLGPLLKPSLTFSVNVIVELITGKDNFINKDFRLVDVRDVALAHIKA 245

Query: 291 YEGMKENASGRYICFDRVISTVDEATKLASE 317
           +E    +A+GRYI    V+ T+++  K+  E
Sbjct: 246 FE--TPSANGRYIIEGPVV-TINDIEKILRE 267

BLAST of Spg039203 vs. TAIR 10
Match: AT5G19440.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 120.2 bits (300), Expect = 3.5e-27
Identity = 81/264 (30.68%), Postives = 132/264 (50.00%), Query Frame = 0

Query: 46  DDAEEKVVCVTSGVSFLGSAIVNELSIHGYSVRVIVDNPEDVNKLEETTS---GRHNVTV 105
           +  E KVVCVT    ++ S +V  L   GY+V+  V +P D  K +   S    +  + +
Sbjct: 3   NSGEGKVVCVTGASGYIASWLVKFLLSRGYTVKASVRDPSDPKKTQHLVSLEGAKERLHL 62

Query: 106 VVAKLTDVNSLVEAFNGCRGVFHTSSSIDPAGVSGYTKVMSEVEMRTTENVMEACARTET 165
             A L +  S   A +GC GVFHT+S           +++ +  ++ T NV+ +CA+  +
Sbjct: 63  FKADLLEQGSFDSAIDGCHGVFHTASPFFNDAKDPQAELI-DPAVKGTLNVLNSCAKASS 122

Query: 166 VKNCVLTSSLLACIWRDQTGESESTGASRIVNHGSWSSLQLCQDKKLWFALGKLKSEKVA 225
           VK  V+TSS+ A  +  +    + T     V+   +S  +LC+  K+W+ L K  +E  A
Sbjct: 123 VKRVVVTSSMAAVGYNGKPRTPDVT-----VDETWFSDPELCEASKMWYVLSKTLAEDAA 182

Query: 226 WKIAQESGLKLATICSALVTGPNFHRRNSTPTMAYLK--GAREMYTNSLLATVDVTKLAK 285
           WK+A+E GL + TI  A+V GP      +T   A L      + + N     V+V  +A 
Sbjct: 183 WKLAKEKGLDIVTINPAMVIGPLLQPTLNTSAAAILNLINGAKTFPNLSFGWVNVKDVAN 242

Query: 286 AHVCVYEGMKENASGRYICFDRVI 305
           AH+  +E    +A+GRY   +RV+
Sbjct: 243 AHIQAFE--VPSANGRYCLVERVV 258

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7016195.13.7e-18089.44Cinnamoyl-CoA reductase-like SNL6 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022938554.18.3e-18089.17cinnamoyl-CoA reductase-like SNL6 [Cucurbita moschata][more]
XP_023551091.11.4e-17989.44cinnamoyl-CoA reductase-like SNL6 [Cucurbita pepo subsp. pepo][more]
XP_022993633.19.2e-17988.46cinnamoyl-CoA reductase-like SNL6 [Cucurbita maxima][more]
XP_022953600.11.9e-17688.43cinnamoyl-CoA reductase-like SNL6 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q0JKZ05.2e-7642.86Cinnamoyl-CoA reductase-like SNL6 OS=Oryza sativa subsp. japonica OX=39947 GN=SN... [more]
A0A059TC021.1e-3334.69Cinnamoyl-CoA reductase 1 OS=Petunia hybrida OX=4102 GN=CCR1 PE=1 SV=1[more]
Q500U83.2e-2530.22Tetraketide alpha-pyrone reductase 1 OS=Arabidopsis thaliana OX=3702 GN=TKPR1 PE... [more]
Q9CA281.6e-2432.42Tetraketide alpha-pyrone reductase 2 OS=Arabidopsis thaliana OX=3702 GN=TKPR2 PE... [more]
Q6K9A22.7e-2429.85Cinnamoyl-CoA reductase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=CCR1 PE=1 ... [more]
Match NameE-valueIdentityDescription
A0A6J1FEE84.0e-18089.17cinnamoyl-CoA reductase-like SNL6 OS=Cucurbita moschata OX=3662 GN=LOC111444756 ... [more]
A0A6J1K2V04.5e-17988.46cinnamoyl-CoA reductase-like SNL6 OS=Cucurbita maxima OX=3661 GN=LOC111489565 PE... [more]
A0A6J1GQ539.3e-17788.43cinnamoyl-CoA reductase-like SNL6 OS=Cucurbita moschata OX=3662 GN=LOC111456087 ... [more]
A0A6J1JPH86.7e-17587.88cinnamoyl-CoA reductase-like SNL6 OS=Cucurbita maxima OX=3661 GN=LOC111487721 PE... [more]
A0A6J1BZP53.9e-16781.82cinnamoyl-CoA reductase-like SNL6 OS=Momordica charantia OX=3673 GN=LOC111006177... [more]
Match NameE-valueIdentityDescription
AT5G14700.11.2e-9651.46NAD(P)-binding Rossmann-fold superfamily protein [more]
AT2G23910.19.6e-4134.09NAD(P)-binding Rossmann-fold superfamily protein [more]
AT4G30470.19.6e-4134.30NAD(P)-binding Rossmann-fold superfamily protein [more]
AT1G09510.14.9e-2931.37NAD(P)-binding Rossmann-fold superfamily protein [more]
AT5G19440.13.5e-2730.68NAD(P)-binding Rossmann-fold superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR016040NAD(P)-binding domainPFAMPF13460NAD_binding_10coord: 62..175
e-value: 1.1E-10
score: 41.7
NoneNo IPR availableGENE3D3.40.50.720coord: 43..361
e-value: 1.8E-61
score: 210.1
NoneNo IPR availablePANTHERPTHR10366NAD DEPENDENT EPIMERASE/DEHYDRATASEcoord: 38..359
NoneNo IPR availablePANTHERPTHR10366:SF483CINNAMOYL COA REDUCTASE-LIKE PROTEINcoord: 38..359
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 49..341

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg039203.1Spg039203.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010190 cytochrome b6f complex assembly
biological_process GO:0006694 steroid biosynthetic process
cellular_component GO:0009507 chloroplast
molecular_function GO:0003854 3-beta-hydroxy-delta5-steroid dehydrogenase activity