Spg025977 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg025977
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionProtein kinase domain-containing protein
Locationscaffold7: 290469 .. 292039 (-)
RNA-Seq ExpressionSpg025977
SyntenySpg025977
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCAGAGTTTCTTCAATCTCCACCGTGCGGCGGATTCTGCAAATGGAAATGTCTTAGTGTTTACATCAGAAGACTTGAAAGCATTTACCAACAATTTCAACGGCCATAACCTGATCGGAATCACTCAGTCCGGCAGATTTTACAGAGGGCAGCTCCGATCATCTTGGTCGACTGCAACAGAAGCTCAAAACATGACAGTAAAAATATGGGATCAAAACAACGAATGCTTGGTTCAAGTGAGTCGTCAGATTTTAAGTTTATAACCTCCATTTGCAGACATGGCTGTGTTTTCGAAGCTTGAAATTTTGTCTTTTCAGGAAGAAGTGAAGTTATCAACGCACCCATCTCTAAATGGCCATCCCAGTTTGGCGAAATTGATTGGTTATTGCTGTGAAGATGAAGTGAAGGGTGTCGTTTATGATCTCAATCCATTGGGTTCCTTGCAAAATCTTACGGCAAGAGGTAAATTACACGTCAATTGCCATAAAACCAATGCCTTCGAAACTCTCTATTAATTTTGAAATATTTTTCTGTAGATGAGCTTAATTGGCTCCAAAGAGTTGATGTAATTCTCGAACTTGCTCGGCTGCTGGACTTCATTCACAGTCAGGAAAAGCAAAGCCTGGCCTTCAAGTTTAGAGCATCAAATATACTGCTTGATTGGGTCAGAATAGTTGCTTTCTAGGCTTTGAACAATGAAGAAAAACTACTGCTATCTCATGAATCTGTATGTTTCAGTCCATGCAACTTCAAGATTGTTAACCTGTTTATTTGAACAGGAATGCAAGCCGAAACTATGCGGATTCGGGCCGGTTAGCAATGGGATGGACACTGACCTCAATAAACTTTACAAGTTTGCACAGAATCCTTCTGCCTATGTTCATCCATCTACTATAAGAGGTACTGAATTTGGTGTGAATACAAGTGAGCAGATAAACAATCTCCAAGAAGTCAGTTAAAAAATGTCAACGTGCTTGTTAAATAGGTATTGATCAAGATTAGATAACTTTCAAACATAATATAGAAAAACCAATGTACCACATAAACAATCCAATTGGCAAACTAAACACTAGTTTATACAAAGTAAACTAGTCTGAATCTCTCAATTCTCAACTTCCTCCACCAAAAGAAAATGAAGTTTTGTTCCTTGAAATTTATTAAATGAAGCATTGCAGGTGGGAGTCAAGGGGAAATGAGCTCTGATGTATATTCCTTGGGGGAAATTCTGCTGGGACTTATAGCCAAGAGAGAGTTTGAACCAGAGAAACTGGATAAACAAAGTCATCACCGGGTTGTGAACAGCTTAGTGAGCATTTGGGCAAAGAATGAATACAGACCAAATGCCTCACTTGTCCATGAAACCTTGCAAAAAGATTGGGGTTACAGTGCTGAAGAGGGAGTTGAACTCACCAAGCTAGCAATGCACAGTATAGAATTCTTCCCAAGAAATCGTCCGAGCATAAAGCAAATACTTCAGCGTCTCGAAGGGTTGCAAGTTACTCAACGTCTTTCTGATGTCAGACCCAGGAAAAAGGAAAGAAAATTCCCTAGCAATGCCATGGGCATATGA

mRNA sequence

ATGCAGAGTTTCTTCAATCTCCACCGTGCGGCGGATTCTGCAAATGGAAATGTCTTAGTGTTTACATCAGAAGACTTGAAAGCATTTACCAACAATTTCAACGGCCATAACCTGATCGGAATCACTCAGTCCGGCAGATTTTACAGAGGGCAGCTCCGATCATCTTGGTCGACTGCAACAGAAGCTCAAAACATGACAGTAAAAATATGGGATCAAAACAACGAATGCTTGGTTCAAGAAGAAGTGAAGTTATCAACGCACCCATCTCTAAATGGCCATCCCAGTTTGGCGAAATTGATTGGTTATTGCTGTGAAGATGAAGTGAAGGGTGTCGTTTATGATCTCAATCCATTGGGTTCCTTGCAAAATCTTACGGCAAGAGATGAGCTTAATTGGCTCCAAAGAGTTGATGTAATTCTCGAACTTGCTCGGCTGCTGGACTTCATTCACAGTCAGGAAAAGCAAAGCCTGGCCTTCAAGTTTAGAGCATCAAATATACTGCTTGATTGGGAATGCAAGCCGAAACTATGCGGATTCGGGCCGGTTAGCAATGGGATGGACACTGACCTCAATAAACTTTACAAGTTTGCACAGAATCCTTCTGCCTATGTTCATCCATCTACTATAAGAGGTGGGAGTCAAGGGGAAATGAGCTCTGATGTATATTCCTTGGGGGAAATTCTGCTGGGACTTATAGCCAAGAGAGAGTTTGAACCAGAGAAACTGGATAAACAAAGTCATCACCGGGTTGTGAACAGCTTAGTGAGCATTTGGGCAAAGAATGAATACAGACCAAATGCCTCACTTGTCCATGAAACCTTGCAAAAAGATTGGGGTTACAGTGCTGAAGAGGGAGTTGAACTCACCAAGCTAGCAATGCACAGTATAGAATTCTTCCCAAGAAATCGTCCGAGCATAAAGCAAATACTTCAGCGTCTCGAAGGGTTGCAAGTTACTCAACGTCTTTCTGATGTCAGACCCAGGAAAAAGGAAAGAAAATTCCCTAGCAATGCCATGGGCATATGA

Coding sequence (CDS)

ATGCAGAGTTTCTTCAATCTCCACCGTGCGGCGGATTCTGCAAATGGAAATGTCTTAGTGTTTACATCAGAAGACTTGAAAGCATTTACCAACAATTTCAACGGCCATAACCTGATCGGAATCACTCAGTCCGGCAGATTTTACAGAGGGCAGCTCCGATCATCTTGGTCGACTGCAACAGAAGCTCAAAACATGACAGTAAAAATATGGGATCAAAACAACGAATGCTTGGTTCAAGAAGAAGTGAAGTTATCAACGCACCCATCTCTAAATGGCCATCCCAGTTTGGCGAAATTGATTGGTTATTGCTGTGAAGATGAAGTGAAGGGTGTCGTTTATGATCTCAATCCATTGGGTTCCTTGCAAAATCTTACGGCAAGAGATGAGCTTAATTGGCTCCAAAGAGTTGATGTAATTCTCGAACTTGCTCGGCTGCTGGACTTCATTCACAGTCAGGAAAAGCAAAGCCTGGCCTTCAAGTTTAGAGCATCAAATATACTGCTTGATTGGGAATGCAAGCCGAAACTATGCGGATTCGGGCCGGTTAGCAATGGGATGGACACTGACCTCAATAAACTTTACAAGTTTGCACAGAATCCTTCTGCCTATGTTCATCCATCTACTATAAGAGGTGGGAGTCAAGGGGAAATGAGCTCTGATGTATATTCCTTGGGGGAAATTCTGCTGGGACTTATAGCCAAGAGAGAGTTTGAACCAGAGAAACTGGATAAACAAAGTCATCACCGGGTTGTGAACAGCTTAGTGAGCATTTGGGCAAAGAATGAATACAGACCAAATGCCTCACTTGTCCATGAAACCTTGCAAAAAGATTGGGGTTACAGTGCTGAAGAGGGAGTTGAACTCACCAAGCTAGCAATGCACAGTATAGAATTCTTCCCAAGAAATCGTCCGAGCATAAAGCAAATACTTCAGCGTCTCGAAGGGTTGCAAGTTACTCAACGTCTTTCTGATGTCAGACCCAGGAAAAAGGAAAGAAAATTCCCTAGCAATGCCATGGGCATATGA

Protein sequence

MQSFFNLHRAADSANGNVLVFTSEDLKAFTNNFNGHNLIGITQSGRFYRGQLRSSWSTATEAQNMTVKIWDQNNECLVQEEVKLSTHPSLNGHPSLAKLIGYCCEDEVKGVVYDLNPLGSLQNLTARDELNWLQRVDVILELARLLDFIHSQEKQSLAFKFRASNILLDWECKPKLCGFGPVSNGMDTDLNKLYKFAQNPSAYVHPSTIRGGSQGEMSSDVYSLGEILLGLIAKREFEPEKLDKQSHHRVVNSLVSIWAKNEYRPNASLVHETLQKDWGYSAEEGVELTKLAMHSIEFFPRNRPSIKQILQRLEGLQVTQRLSDVRPRKKERKFPSNAMGI
Homology
BLAST of Spg025977 vs. NCBI nr
Match: XP_022945250.1 (serine/threonine-protein kinase BIK1-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 563.5 bits (1451), Expect = 1.2e-156
Identity = 284/349 (81.38%), Postives = 309/349 (88.54%), Query Frame = 0

Query: 1   MQSFFNLHRAADSANGNVLVFTSEDLKAFTNNFNGHNLIGITQSGRFYRGQLRSSWSTAT 60
           MQSFFNLHRAADSANGNVL+FTSEDLK  T+NF+ ++LIGITQSGRFYRGQLRS+WST T
Sbjct: 4   MQSFFNLHRAADSANGNVLIFTSEDLKTLTSNFHSNSLIGITQSGRFYRGQLRSAWSTGT 63

Query: 61  EAQNMTVKIWDQ--------NNECLVQEEVKLSTHPSLNGHPSLAKLIGYCCEDEVKGVV 120
           EA+N+TVKIWDQ         NE LV+EEVKLSTHPSLNGHPSLAKLIGYCCEDEVKGVV
Sbjct: 64  EARNLTVKIWDQKPEFDNATTNEFLVREEVKLSTHPSLNGHPSLAKLIGYCCEDEVKGVV 123

Query: 121 YDLNPLGSLQNLTARDELNWLQRVDVILELARLLDFIHSQEKQSLAFKFRASNILLDWEC 180
           YDL+PLGSLQNL ARD+LNWLQRV+VILELARLLDFIHSQEKQSL  KFRASNILLDWEC
Sbjct: 124 YDLDPLGSLQNLVARDDLNWLQRVEVILELARLLDFIHSQEKQSLPIKFRASNILLDWEC 183

Query: 181 KPKLCGFGPVSNGMDTDLNKLYKFAQNPSAYVHPSTIRGGSQGEMSSDVYSLGEILLGLI 240
           KPKLCG G        DLN    FAQNPS+Y H ST RGGSQG+MS+D+YSLGEILLGLI
Sbjct: 184 KPKLCGTG-------ADLN---NFAQNPSSYFHLST-RGGSQGKMSTDIYSLGEILLGLI 243

Query: 241 AKREFEPEKLDKQSHHRVVNSLVSIWAKNEYRPNASLVHETLQKDWGYSAEEGVELTKLA 300
           AKR +EPEKL++++HH VVNSLVS WAKNEYRPN SLVHETLQKDWGY+AEEGVELT+LA
Sbjct: 244 AKRAYEPEKLERENHHLVVNSLVSNWAKNEYRPNGSLVHETLQKDWGYTAEEGVELTELA 303

Query: 301 MHSIEFFPRNRPSIKQILQRLEGLQVTQRLSDVRPRKKERKFPSNAMGI 342
           +HSIEFFPRNRPSIKQILQ LEGLQVTQRLSD RPRKKERK PS+AMGI
Sbjct: 304 IHSIEFFPRNRPSIKQILQHLEGLQVTQRLSDTRPRKKERKSPSSAMGI 341

BLAST of Spg025977 vs. NCBI nr
Match: XP_022966807.1 (probable serine/threonine-protein kinase CST [Cucurbita maxima])

HSP 1 Score: 563.5 bits (1451), Expect = 1.2e-156
Identity = 281/349 (80.52%), Postives = 310/349 (88.83%), Query Frame = 0

Query: 1   MQSFFNLHRAADSANGNVLVFTSEDLKAFTNNFNGHNLIGITQSGRFYRGQLRSSWSTAT 60
           MQS FNLHRAADSANGNVL+FTSEDLK  T+NF+G++LIGITQS RFYRGQLR++WST T
Sbjct: 4   MQSSFNLHRAADSANGNVLIFTSEDLKTLTSNFHGNSLIGITQSCRFYRGQLRAAWSTGT 63

Query: 61  EAQNMTVKIWDQ--------NNECLVQEEVKLSTHPSLNGHPSLAKLIGYCCEDEVKGVV 120
           EA+N+TVKIWDQ         NE LV+EEVKLSTH SLNGH SLAKLIGYCCEDEVKGVV
Sbjct: 64  EARNLTVKIWDQKPEFDNAITNEFLVREEVKLSTHSSLNGHRSLAKLIGYCCEDEVKGVV 123

Query: 121 YDLNPLGSLQNLTARDELNWLQRVDVILELARLLDFIHSQEKQSLAFKFRASNILLDWEC 180
           YD +PLGSLQNL ARD+LNWLQRV+VILELARLLDFIHSQEKQSL  KFRASNILLDWEC
Sbjct: 124 YDFDPLGSLQNLVARDDLNWLQRVEVILELARLLDFIHSQEKQSLPIKFRASNILLDWEC 183

Query: 181 KPKLCGFGPVSNGMDTDLNKLYKFAQNPSAYVHPSTIRGGSQGEMSSDVYSLGEILLGLI 240
           KPKLCG   +SNG   DLN    FAQNPS+Y H ST RGGSQG+MS+D++SLGEILLGLI
Sbjct: 184 KPKLCGLEVISNGAGADLN---NFAQNPSSYFHLST-RGGSQGKMSTDIFSLGEILLGLI 243

Query: 241 AKREFEPEKLDKQSHHRVVNSLVSIWAKNEYRPNASLVHETLQKDWGYSAEEGVELTKLA 300
           AKR++EPEKL++++HHRVVNSLVS WAKNEYRPN SLVHETLQKDWGY+ EEGVELT+LA
Sbjct: 244 AKRDYEPEKLERENHHRVVNSLVSNWAKNEYRPNGSLVHETLQKDWGYTVEEGVELTELA 303

Query: 301 MHSIEFFPRNRPSIKQILQRLEGLQVTQRLSDVRPRKKERKFPSNAMGI 342
           +HSIEFFPRNRPSIKQILQRLEGLQVTQRLSD RPRKKERK PS+AMGI
Sbjct: 304 IHSIEFFPRNRPSIKQILQRLEGLQVTQRLSDTRPRKKERKSPSSAMGI 348

BLAST of Spg025977 vs. NCBI nr
Match: KAG6573595.1 (Serine/threonine-protein kinase PBL13, partial [Cucurbita argyrosperma subsp. sororia] >KAG7012721.1 Serine/threonine-protein kinase PBL13, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 558.5 bits (1438), Expect = 3.9e-155
Identity = 282/349 (80.80%), Postives = 307/349 (87.97%), Query Frame = 0

Query: 1   MQSFFNLHRAADSANGNVLVFTSEDLKAFTNNFNGHNLIGITQSGRFYRGQLRSSWSTAT 60
           MQSFFNLHRAADSANGNVL+FTSEDLK  T+NF+ ++LIGITQSGRFYRGQLRS+WST T
Sbjct: 4   MQSFFNLHRAADSANGNVLIFTSEDLKTLTSNFHSNSLIGITQSGRFYRGQLRSAWSTGT 63

Query: 61  EAQNMTVKIWDQ--------NNECLVQEEVKLSTHPSLNGHPSLAKLIGYCCEDEVKGVV 120
           EA+N+TVKIWDQ         NE LV+EEVKLS HPSLNGHPSLAKLIGYCCEDEVKGVV
Sbjct: 64  EARNLTVKIWDQKPEFDNATTNEFLVREEVKLSAHPSLNGHPSLAKLIGYCCEDEVKGVV 123

Query: 121 YDLNPLGSLQNLTARDELNWLQRVDVILELARLLDFIHSQEKQSLAFKFRASNILLDWEC 180
           YDL+PLGSLQNL ARD+LNWLQRV+VILELARLLDFIHSQEKQSL  KFRASNILLDWEC
Sbjct: 124 YDLDPLGSLQNLVARDDLNWLQRVEVILELARLLDFIHSQEKQSLPIKFRASNILLDWEC 183

Query: 181 KPKLCGFGPVSNGMDTDLNKLYKFAQNPSAYVHPSTIRGGSQGEMSSDVYSLGEILLGLI 240
           KPKLCG G        DLN    FAQNPS+Y H ST RGGSQG+MS+D+YSLGEILLGLI
Sbjct: 184 KPKLCGTG-------ADLN---NFAQNPSSYFHLST-RGGSQGKMSTDIYSLGEILLGLI 243

Query: 241 AKREFEPEKLDKQSHHRVVNSLVSIWAKNEYRPNASLVHETLQKDWGYSAEEGVELTKLA 300
           AKR +EPEKL++++HH VVNSLVS WAKNEYRPN SLVHETLQKDWGY+AEEGVELT+LA
Sbjct: 244 AKRAYEPEKLERENHHLVVNSLVSNWAKNEYRPNGSLVHETLQKDWGYTAEEGVELTELA 303

Query: 301 MHSIEFFPRNRPSIKQILQRLEGLQVTQRLSDVRPRKKERKFPSNAMGI 342
           +  IEFFPRNRPSIKQILQRLEGLQVTQRLSD RPRKKERK PS+AMGI
Sbjct: 304 IQCIEFFPRNRPSIKQILQRLEGLQVTQRLSDTRPRKKERKSPSSAMGI 341

BLAST of Spg025977 vs. NCBI nr
Match: XP_022945248.1 (putative receptor-like protein kinase At1g72540 isoform X1 [Cucurbita moschata])

HSP 1 Score: 549.7 bits (1415), Expect = 1.8e-152
Identity = 284/374 (75.94%), Postives = 309/374 (82.62%), Query Frame = 0

Query: 1   MQSFFNLHRAADSANGNVLVFTSEDLKAFTNNFNGHNLIGITQSGRFYRGQLRSSWSTAT 60
           MQSFFNLHRAADSANGNVL+FTSEDLK  T+NF+ ++LIGITQSGRFYRGQLRS+WST T
Sbjct: 4   MQSFFNLHRAADSANGNVLIFTSEDLKTLTSNFHSNSLIGITQSGRFYRGQLRSAWSTGT 63

Query: 61  EAQNMTVKIWDQ--------NNECLVQEEVKLSTHPSLNGHPSLAKLIGYCCEDEVKGVV 120
           EA+N+TVKIWDQ         NE LV+EEVKLSTHPSLNGHPSLAKLIGYCCEDEVKGVV
Sbjct: 64  EARNLTVKIWDQKPEFDNATTNEFLVREEVKLSTHPSLNGHPSLAKLIGYCCEDEVKGVV 123

Query: 121 YDLNPLGSLQNLTAR-------------------------DELNWLQRVDVILELARLLD 180
           YDL+PLGSLQNL AR                         D+LNWLQRV+VILELARLLD
Sbjct: 124 YDLDPLGSLQNLVARGNLQVHEPKNHCLRSSVPVFFFLLADDLNWLQRVEVILELARLLD 183

Query: 181 FIHSQEKQSLAFKFRASNILLDWECKPKLCGFGPVSNGMDTDLNKLYKFAQNPSAYVHPS 240
           FIHSQEKQSL  KFRASNILLDWECKPKLCG G        DLN    FAQNPS+Y H S
Sbjct: 184 FIHSQEKQSLPIKFRASNILLDWECKPKLCGTG-------ADLN---NFAQNPSSYFHLS 243

Query: 241 TIRGGSQGEMSSDVYSLGEILLGLIAKREFEPEKLDKQSHHRVVNSLVSIWAKNEYRPNA 300
           T RGGSQG+MS+D+YSLGEILLGLIAKR +EPEKL++++HH VVNSLVS WAKNEYRPN 
Sbjct: 244 T-RGGSQGKMSTDIYSLGEILLGLIAKRAYEPEKLERENHHLVVNSLVSNWAKNEYRPNG 303

Query: 301 SLVHETLQKDWGYSAEEGVELTKLAMHSIEFFPRNRPSIKQILQRLEGLQVTQRLSDVRP 342
           SLVHETLQKDWGY+AEEGVELT+LA+HSIEFFPRNRPSIKQILQ LEGLQVTQRLSD RP
Sbjct: 304 SLVHETLQKDWGYTAEEGVELTELAIHSIEFFPRNRPSIKQILQHLEGLQVTQRLSDTRP 363

BLAST of Spg025977 vs. NCBI nr
Match: XP_022151893.1 (putative receptor-like protein kinase At1g72540 isoform X2 [Momordica charantia])

HSP 1 Score: 527.7 bits (1358), Expect = 7.4e-146
Identity = 264/329 (80.24%), Postives = 283/329 (86.02%), Query Frame = 0

Query: 10  AADSANGNVLVFTSEDLKAFTNNFNGHNLIGITQSGRFYRGQLRSSWSTATEAQNMTVKI 69
           +ADSAN  V VFTS+DLKAFT+NFNG NLIGIT SG  YRGQLRSSWSTA EA+N+TVKI
Sbjct: 41  SADSANMKVSVFTSQDLKAFTDNFNGKNLIGITHSGMLYRGQLRSSWSTAEEARNVTVKI 100

Query: 70  WDQNN----ECLVQEEVKLSTHPSLNGHPSLAKLIGYCCEDEVKGVVYDLNPLGSLQNLT 129
           WDQ      ECLV+EEVKL T PSL GHPS A L+GYCCEDEVKGVVYDLNPLG LQNL 
Sbjct: 101 WDQKPEFACECLVREEVKLLTQPSLKGHPSFANLVGYCCEDEVKGVVYDLNPLGFLQNLM 160

Query: 130 ARDELNWLQRVDVILELARLLDFIHSQEKQSLAFKFRASNILLDWECKPKLCGFGPVSNG 189
            RD  NWLQRVDVILELARLLDFIH QEK SLAF FRASNILLDWECKPKLCGF P SN 
Sbjct: 161 TRDNFNWLQRVDVILELARLLDFIHWQEKPSLAFDFRASNILLDWECKPKLCGFAPNSNE 220

Query: 190 MDTDLNKLYKFAQNPSAYVHPSTIRGGSQGEMSSDVYSLGEILLGLIAKREFEPEKLDKQ 249
             +DLNK YKFAQ PS Y HPS IRGGS+G+MSSDVYSLGEILLGLIAKR+ E EKL+KQ
Sbjct: 221 TVSDLNKPYKFAQIPSGYSHPS-IRGGSRGKMSSDVYSLGEILLGLIAKRDLEHEKLEKQ 280

Query: 250 SHHRVVNSLVSIWAKNEYRPNASLVHETLQKDWGYSAEEGVELTKLAMHSIEFFPRNRPS 309
            HH+VVNSLVSIWAKNEYRPNASLVHETLQ+DWGY+AEEGV +T+LAMH IEFFP NRPS
Sbjct: 281 DHHQVVNSLVSIWAKNEYRPNASLVHETLQEDWGYNAEEGVRITELAMHCIEFFPSNRPS 340

Query: 310 IKQILQRLEGLQVTQRLSDVRPRKKERKF 335
            KQ+LQ LEGLQV QRL+DVRPRKKERKF
Sbjct: 341 TKQVLQHLEGLQVAQRLTDVRPRKKERKF 368

BLAST of Spg025977 vs. ExPASy Swiss-Prot
Match: Q9CAH1 (Putative receptor-like protein kinase At1g72540 OS=Arabidopsis thaliana OX=3702 GN=At1g72540 PE=2 SV=1)

HSP 1 Score: 124.8 bits (312), Expect = 1.9e-27
Identity = 94/327 (28.75%), Postives = 157/327 (48.01%), Query Frame = 0

Query: 3   SFFNLHRAADSANGNVLVFTSEDLKAFTNNFNGHNLIGITQSGRFYRGQLRSSWSTATEA 62
           S  +L+  ++S   N+ +FT E+LK  T  F+ +N +G    G  Y+G +  S  T  + 
Sbjct: 54  STISLNDFSNSFFINIHIFTYEELKTITQGFSKYNFLGEGGFGEVYKGFVDDSLKTGLKD 113

Query: 63  QNMTVKIWDQNN---------ECLVQEEVKLSTHPSLNGHPSLAKLIGYCCEDEVKGVVY 122
           Q + VK   +           E ++  ++K         HP L  L+GYCCED+ + +VY
Sbjct: 114 QPVAVKALKREGGQGHREWLAEVIILGQLK---------HPHLVNLVGYCCEDDERLLVY 173

Query: 123 DLNPLGSLQNLTAR---DELNWLQRVDVILELARLLDFIHSQEKQSLAFKFRASNILLDW 182
           +    G+L++   +     L WL RV ++L  A+ L+F+H QEK  +   F+ SNILL  
Sbjct: 174 EYMERGNLEDHLFQKYGGALPWLTRVKILLGAAKGLEFLHKQEKPVIYRDFKPSNILLSS 233

Query: 183 ECKPKLCGFGPVSNGMDTDLNKLYKFAQNPSAYVHPSTIRGGSQGEMSSDVYSLGEILLG 242
           +   KL  FG  ++G + + +   K       Y  P  I  G+   M SDV+S G +LL 
Sbjct: 234 DFSSKLSDFGLATDGSEEEDSNFTKSVMGTEGYAAPEYISAGNLTTM-SDVFSFGVVLLE 293

Query: 243 LIAKREFEPEKLDKQSHHRVVNSLVSIWAKNEYR-PN--ASLVHETLQKDWGYSAEEGVE 302
           ++  R+   EK   Q    +V      WA+   + PN    ++  +L+    YS E   +
Sbjct: 294 MLTARK-AVEKYRAQRGRNLVE-----WARPMLKDPNKLERIIDPSLEGK--YSVEGIRK 353

Query: 303 LTKLAMHSIEFFPRNRPSIKQILQRLE 315
              LA   +   P++RP++  +++ LE
Sbjct: 354 AAALAYQCLSHNPKSRPTMTTVVKTLE 362

BLAST of Spg025977 vs. ExPASy Swiss-Prot
Match: O48814 (Serine/threonine-protein kinase BIK1 OS=Arabidopsis thaliana OX=3702 GN=BIK1 PE=1 SV=1)

HSP 1 Score: 122.9 bits (307), Expect = 7.2e-27
Identity = 102/328 (31.10%), Postives = 153/328 (46.65%), Query Frame = 0

Query: 10  AADSANGNVL------VFTSEDLKAFTNNFNGHNLIGITQSGRFYRGQLRSSWSTATE-A 69
           AA    G +L       FT  +LK  T NF   ++IG    G  ++G L  S  T T+  
Sbjct: 38  AAQKTEGEILSSTPVKSFTFNELKLATRNFRPDSVIGEGGFGCVFKGWLDESTLTPTKPG 97

Query: 70  QNMTVKIWDQNNECLVQEEVKLSTHPSLN--GHPSLAKLIGYCCEDEVKGVVYDLNPLGS 129
             + + +   N E        L+    L    HP+L KLIGYC EDE + +VY+    GS
Sbjct: 98  TGLVIAVKKLNQEGFQGHREWLTEINYLGQLSHPNLVKLIGYCLEDEHRLLVYEFMQKGS 157

Query: 130 LQNLTAR-----DELNWLQRVDVILELARLLDFIHSQEKQSLAFKFRASNILLDWECKPK 189
           L+N   R       L W  RV+V L+ A+ L F+HS   + +    +ASNILLD +   K
Sbjct: 158 LENHLFRRGAYFKPLPWFLRVNVALDAAKGLAFLHSDPVKVIYRDIKASNILLDADYNAK 217

Query: 190 LCGFGPVSNGMDTDLNKLYKFAQNPSAYVHPSTIRGGSQGEMSSDVYSLGEILLGLIA-K 249
           L  FG   +G   DL+ +         Y  P  +  G      SDVYS G +LL +++ K
Sbjct: 218 LSDFGLARDGPMGDLSYVSTRVMGTYGYAAPEYMSSGHL-NARSDVYSFGVLLLEILSGK 277

Query: 250 REFEPEKLDKQSHHRVVNSLVSIWAKNEYRPNASLVHETL-----QKDWGYSAEEGVELT 309
           R  +  +  K+       +LV  WA    RP  +   + L     + D  Y  EE V + 
Sbjct: 278 RALDHNRPAKE------ENLVD-WA----RPYLTSKRKVLLIVDNRLDTQYLPEEAVRMA 337

Query: 310 KLAMHSIEFFPRNRPSIKQILQRLEGLQ 318
            +A+  + F P++RP++ Q+++ L+ LQ
Sbjct: 338 SVAVQCLSFEPKSRPTMDQVVRALQQLQ 353

BLAST of Spg025977 vs. ExPASy Swiss-Prot
Match: F4JZW1 (Serine/threonine-protein kinase PBL13 OS=Arabidopsis thaliana OX=3702 GN=PBL13 PE=1 SV=1)

HSP 1 Score: 120.6 bits (301), Expect = 3.6e-26
Identity = 93/313 (29.71%), Postives = 145/313 (46.33%), Query Frame = 0

Query: 14  ANGNVLVFTSEDLKAFTNNFNGHNLIGITQSGRFYRGQLRSSWSTATEAQNMTVKIWDQN 73
           A  ++ VFT  +L+  T +F+  N +G    G  ++G +        +AQ + VK+ D +
Sbjct: 57  AGSDLHVFTQAELRVITQSFSSSNFLGEGGFGPVHKGFIDDKLRPGLKAQPVAVKLLDLD 116

Query: 74  NECLVQEEVKLSTHPSLNG---HPSLAKLIGYCCEDEVKGVVYDLNPLGSLQNLTARD-- 133
               +Q   +  T     G   HP+L KLIGYCCE+  + +VY+  P GSL++   R   
Sbjct: 117 G---LQGHREFMTEVMCLGKLKHPNLVKLIGYCCEEAHRLLVYEFMPRGSLESQLFRRCS 176

Query: 134 -ELNWLQRVDVILELARLLDFIHSQEKQSLAFKFRASNILLDWECKPKLCGFGPVSNGMD 193
             L W  R+++  E A+ L F+H  EK  +   F+ASNILLD +   KL  FG   +G  
Sbjct: 177 LPLPWTTRLNIAYEAAKGLQFLHEAEKPIIYRDFKASNILLDSDYTAKLSDFGLAKDGPQ 236

Query: 194 TDLNKLYKFAQNPSAYVHPSTIRGGSQGEMSSDVYSLGEILLGLIAKREFEPEKLDKQSH 253
            D   +         Y  P  I  G      SDVYS G +LL L+  R    + +D    
Sbjct: 237 GDDTHVSTRVMGTQGYAAPEYIMTGHL-TAKSDVYSFGVVLLELLTGR----KSVDIARS 296

Query: 254 HRVVNSLVSIWAK---NEYRPNASLVHETLQKDWGYSAEEGVELTKLAMHSIEFFPRNRP 313
            R   +LV  WA+   N+ R    ++   L+    YS     +   LA   + + P+ RP
Sbjct: 297 SR-KETLVE-WARPMLNDARKLGRIMDPRLEDQ--YSETGARKAATLAYQCLRYRPKTRP 356

Query: 314 SIKQILQRLEGLQ 318
            I  ++  L+ ++
Sbjct: 357 DISTVVSVLQDIK 357

BLAST of Spg025977 vs. ExPASy Swiss-Prot
Match: P46573 (Probable serine/threonine-protein kinase PBL10 OS=Arabidopsis thaliana OX=3702 GN=PBL10 PE=1 SV=2)

HSP 1 Score: 119.4 bits (298), Expect = 7.9e-26
Identity = 100/338 (29.59%), Postives = 156/338 (46.15%), Query Frame = 0

Query: 17  NVLVFTSEDLKAFTNNFNGHNLIGITQSGRFYRGQLRSSWSTATEAQN---MTVKIWDQN 76
           N+  FT  +LKA T NF   +++G    G  ++G +     TA++      + VK  +Q+
Sbjct: 53  NLKSFTFAELKAATRNFRPDSVLGEGGFGSVFKGWIDEQTLTASKPGTGVVIAVKKLNQD 112

Query: 77  NECLVQEEVKLSTHPSLNGHPSLAKLIGYCCEDEVKGVVYDLNPLGSLQNLTAR-----D 136
                QE +    +     HP+L KLIGYC EDE + +VY+  P GSL+N   R      
Sbjct: 113 GWQGHQEWLAEVNYLGQFSHPNLVKLIGYCLEDEHRLLVYEFMPRGSLENHLFRRGSYFQ 172

Query: 137 ELNWLQRVDVILELARLLDFIHSQEKQSLAFKFRASNILLDWECKPKLCGFGPVSNGMDT 196
            L+W  R+ V L  A+ L F+H+ E   +   F+ SNILLD E   KL  FG   +G   
Sbjct: 173 PLSWTLRLKVALGAAKGLAFLHNAETSVIYRDFKTSNILLDSEYNAKLSDFGLAKDGPTG 232

Query: 197 DLNKLYKFAQNPSAYVHPSTIRGGSQGEMSSDVYSLGEILLGLIAKREFEPEKLDKQSHH 256
           D + +         Y  P  +  G      SDVYS G +LL +++ R    +K       
Sbjct: 233 DKSHVSTRIMGTYGYAAPEYLATGHL-TTKSDVYSYGVVLLEVLSGRR-AVDKNRPPGEQ 292

Query: 257 RVVNSLVSIWAK---NEYRPNASLVHETLQKDWGYSAEEGVELTKLAMHSIEFFPRNRPS 316
           ++V      WA+      R    ++   LQ    YS EE  ++  LA+  + F  + RP+
Sbjct: 293 KLVE-----WARPLLANKRKLFRVIDNRLQDQ--YSMEEACKVATLALRCLTFEIKLRPN 352

Query: 317 IKQILQRLEGLQVTQ----RLSDVRPRKKERKFPSNAM 340
           + +++  LE +Q       R  D+  R+  R+  S A+
Sbjct: 353 MNEVVSHLEHIQTLNEAGGRNIDMVQRRMRRRSDSVAI 381

BLAST of Spg025977 vs. ExPASy Swiss-Prot
Match: Q9FM85 (Probable serine/threonine-protein kinase PBL16 OS=Arabidopsis thaliana OX=3702 GN=PBL16 PE=1 SV=1)

HSP 1 Score: 117.9 bits (294), Expect = 2.3e-25
Identity = 96/318 (30.19%), Postives = 147/318 (46.23%), Query Frame = 0

Query: 12  DSANGNVLVFTSEDLKAFTNNFNGHNLIGITQSGRFYRGQLRSSW--STATEAQNMTVKI 71
           DSA   ++ FT E+LK  T+NF    ++G    G  Y+G ++         E   + VK+
Sbjct: 55  DSAANPLIAFTYEELKNITSNFRQDRVLGGGGFGSVYKGFIKEDLGDQEVPEPLPVAVKV 114

Query: 72  WDQNNECLVQEE-VKLSTHPSLNGHPSLAKLIGYCCEDEVKGVVYDLNPLGSLQ-NLTAR 131
            D +N      E +          HP+L KLIGYCCED  + ++Y+    GS++ NL +R
Sbjct: 115 HDGDNSFQGHREWLAEVIFLGQLSHPNLVKLIGYCCEDNHRVLIYEYMARGSVENNLFSR 174

Query: 132 D--ELNWLQRVDVILELARLLDFIHSQEKQSLAFKFRASNILLDWECKPKLCGFGPVSNG 191
               L+W  R+ +    A+ L F+H  +K  +   F+ SNILLD +   KL  FG   +G
Sbjct: 175 VLLPLSWAIRMKIAFGAAKGLAFLHEAKKPVIYRDFKTSNILLDMDYNAKLSDFGLAKDG 234

Query: 192 MDTDLNKLYKFAQNPSAYVHPSTIRGGSQGEMSSDVYSLGEILLGLIAKREFEPEKLDKQ 251
              D + +         Y  P  I  G      SDVYS G +LL L+  R    + LDK 
Sbjct: 235 PVGDKSHVSTRIMGTYGYAAPEYIMTGHL-TPGSDVYSFGVVLLELLTGR----KSLDKS 294

Query: 252 SHHRVVNSLVSIWA---KNEYRPNASLVHETLQKDWGYSAEEGVELTKLAMHSIEFFPRN 311
              R  N +   WA     E +   ++V   +  ++   A +  +   LA H +   P+ 
Sbjct: 295 RPTREQNLI--DWALPLLKEKKKVLNIVDPKMNCEYPVKAVQ--KAAMLAYHCLNRNPKA 354

Query: 312 RPSIKQILQRLEGLQVTQ 321
           RP ++ I+  LE LQ T+
Sbjct: 355 RPLMRDIVDSLEPLQATE 363

BLAST of Spg025977 vs. ExPASy TrEMBL
Match: A0A6J1HNY6 (probable serine/threonine-protein kinase CST OS=Cucurbita maxima OX=3661 GN=LOC111466402 PE=4 SV=1)

HSP 1 Score: 563.5 bits (1451), Expect = 5.9e-157
Identity = 281/349 (80.52%), Postives = 310/349 (88.83%), Query Frame = 0

Query: 1   MQSFFNLHRAADSANGNVLVFTSEDLKAFTNNFNGHNLIGITQSGRFYRGQLRSSWSTAT 60
           MQS FNLHRAADSANGNVL+FTSEDLK  T+NF+G++LIGITQS RFYRGQLR++WST T
Sbjct: 4   MQSSFNLHRAADSANGNVLIFTSEDLKTLTSNFHGNSLIGITQSCRFYRGQLRAAWSTGT 63

Query: 61  EAQNMTVKIWDQ--------NNECLVQEEVKLSTHPSLNGHPSLAKLIGYCCEDEVKGVV 120
           EA+N+TVKIWDQ         NE LV+EEVKLSTH SLNGH SLAKLIGYCCEDEVKGVV
Sbjct: 64  EARNLTVKIWDQKPEFDNAITNEFLVREEVKLSTHSSLNGHRSLAKLIGYCCEDEVKGVV 123

Query: 121 YDLNPLGSLQNLTARDELNWLQRVDVILELARLLDFIHSQEKQSLAFKFRASNILLDWEC 180
           YD +PLGSLQNL ARD+LNWLQRV+VILELARLLDFIHSQEKQSL  KFRASNILLDWEC
Sbjct: 124 YDFDPLGSLQNLVARDDLNWLQRVEVILELARLLDFIHSQEKQSLPIKFRASNILLDWEC 183

Query: 181 KPKLCGFGPVSNGMDTDLNKLYKFAQNPSAYVHPSTIRGGSQGEMSSDVYSLGEILLGLI 240
           KPKLCG   +SNG   DLN    FAQNPS+Y H ST RGGSQG+MS+D++SLGEILLGLI
Sbjct: 184 KPKLCGLEVISNGAGADLN---NFAQNPSSYFHLST-RGGSQGKMSTDIFSLGEILLGLI 243

Query: 241 AKREFEPEKLDKQSHHRVVNSLVSIWAKNEYRPNASLVHETLQKDWGYSAEEGVELTKLA 300
           AKR++EPEKL++++HHRVVNSLVS WAKNEYRPN SLVHETLQKDWGY+ EEGVELT+LA
Sbjct: 244 AKRDYEPEKLERENHHRVVNSLVSNWAKNEYRPNGSLVHETLQKDWGYTVEEGVELTELA 303

Query: 301 MHSIEFFPRNRPSIKQILQRLEGLQVTQRLSDVRPRKKERKFPSNAMGI 342
           +HSIEFFPRNRPSIKQILQRLEGLQVTQRLSD RPRKKERK PS+AMGI
Sbjct: 304 IHSIEFFPRNRPSIKQILQRLEGLQVTQRLSDTRPRKKERKSPSSAMGI 348

BLAST of Spg025977 vs. ExPASy TrEMBL
Match: A0A6J1G0E5 (serine/threonine-protein kinase BIK1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111449550 PE=4 SV=1)

HSP 1 Score: 563.5 bits (1451), Expect = 5.9e-157
Identity = 284/349 (81.38%), Postives = 309/349 (88.54%), Query Frame = 0

Query: 1   MQSFFNLHRAADSANGNVLVFTSEDLKAFTNNFNGHNLIGITQSGRFYRGQLRSSWSTAT 60
           MQSFFNLHRAADSANGNVL+FTSEDLK  T+NF+ ++LIGITQSGRFYRGQLRS+WST T
Sbjct: 4   MQSFFNLHRAADSANGNVLIFTSEDLKTLTSNFHSNSLIGITQSGRFYRGQLRSAWSTGT 63

Query: 61  EAQNMTVKIWDQ--------NNECLVQEEVKLSTHPSLNGHPSLAKLIGYCCEDEVKGVV 120
           EA+N+TVKIWDQ         NE LV+EEVKLSTHPSLNGHPSLAKLIGYCCEDEVKGVV
Sbjct: 64  EARNLTVKIWDQKPEFDNATTNEFLVREEVKLSTHPSLNGHPSLAKLIGYCCEDEVKGVV 123

Query: 121 YDLNPLGSLQNLTARDELNWLQRVDVILELARLLDFIHSQEKQSLAFKFRASNILLDWEC 180
           YDL+PLGSLQNL ARD+LNWLQRV+VILELARLLDFIHSQEKQSL  KFRASNILLDWEC
Sbjct: 124 YDLDPLGSLQNLVARDDLNWLQRVEVILELARLLDFIHSQEKQSLPIKFRASNILLDWEC 183

Query: 181 KPKLCGFGPVSNGMDTDLNKLYKFAQNPSAYVHPSTIRGGSQGEMSSDVYSLGEILLGLI 240
           KPKLCG G        DLN    FAQNPS+Y H ST RGGSQG+MS+D+YSLGEILLGLI
Sbjct: 184 KPKLCGTG-------ADLN---NFAQNPSSYFHLST-RGGSQGKMSTDIYSLGEILLGLI 243

Query: 241 AKREFEPEKLDKQSHHRVVNSLVSIWAKNEYRPNASLVHETLQKDWGYSAEEGVELTKLA 300
           AKR +EPEKL++++HH VVNSLVS WAKNEYRPN SLVHETLQKDWGY+AEEGVELT+LA
Sbjct: 244 AKRAYEPEKLERENHHLVVNSLVSNWAKNEYRPNGSLVHETLQKDWGYTAEEGVELTELA 303

Query: 301 MHSIEFFPRNRPSIKQILQRLEGLQVTQRLSDVRPRKKERKFPSNAMGI 342
           +HSIEFFPRNRPSIKQILQ LEGLQVTQRLSD RPRKKERK PS+AMGI
Sbjct: 304 IHSIEFFPRNRPSIKQILQHLEGLQVTQRLSDTRPRKKERKSPSSAMGI 341

BLAST of Spg025977 vs. ExPASy TrEMBL
Match: A0A6J1G0A1 (putative receptor-like protein kinase At1g72540 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111449550 PE=4 SV=1)

HSP 1 Score: 549.7 bits (1415), Expect = 8.8e-153
Identity = 284/374 (75.94%), Postives = 309/374 (82.62%), Query Frame = 0

Query: 1   MQSFFNLHRAADSANGNVLVFTSEDLKAFTNNFNGHNLIGITQSGRFYRGQLRSSWSTAT 60
           MQSFFNLHRAADSANGNVL+FTSEDLK  T+NF+ ++LIGITQSGRFYRGQLRS+WST T
Sbjct: 4   MQSFFNLHRAADSANGNVLIFTSEDLKTLTSNFHSNSLIGITQSGRFYRGQLRSAWSTGT 63

Query: 61  EAQNMTVKIWDQ--------NNECLVQEEVKLSTHPSLNGHPSLAKLIGYCCEDEVKGVV 120
           EA+N+TVKIWDQ         NE LV+EEVKLSTHPSLNGHPSLAKLIGYCCEDEVKGVV
Sbjct: 64  EARNLTVKIWDQKPEFDNATTNEFLVREEVKLSTHPSLNGHPSLAKLIGYCCEDEVKGVV 123

Query: 121 YDLNPLGSLQNLTAR-------------------------DELNWLQRVDVILELARLLD 180
           YDL+PLGSLQNL AR                         D+LNWLQRV+VILELARLLD
Sbjct: 124 YDLDPLGSLQNLVARGNLQVHEPKNHCLRSSVPVFFFLLADDLNWLQRVEVILELARLLD 183

Query: 181 FIHSQEKQSLAFKFRASNILLDWECKPKLCGFGPVSNGMDTDLNKLYKFAQNPSAYVHPS 240
           FIHSQEKQSL  KFRASNILLDWECKPKLCG G        DLN    FAQNPS+Y H S
Sbjct: 184 FIHSQEKQSLPIKFRASNILLDWECKPKLCGTG-------ADLN---NFAQNPSSYFHLS 243

Query: 241 TIRGGSQGEMSSDVYSLGEILLGLIAKREFEPEKLDKQSHHRVVNSLVSIWAKNEYRPNA 300
           T RGGSQG+MS+D+YSLGEILLGLIAKR +EPEKL++++HH VVNSLVS WAKNEYRPN 
Sbjct: 244 T-RGGSQGKMSTDIYSLGEILLGLIAKRAYEPEKLERENHHLVVNSLVSNWAKNEYRPNG 303

Query: 301 SLVHETLQKDWGYSAEEGVELTKLAMHSIEFFPRNRPSIKQILQRLEGLQVTQRLSDVRP 342
           SLVHETLQKDWGY+AEEGVELT+LA+HSIEFFPRNRPSIKQILQ LEGLQVTQRLSD RP
Sbjct: 304 SLVHETLQKDWGYTAEEGVELTELAIHSIEFFPRNRPSIKQILQHLEGLQVTQRLSDTRP 363

BLAST of Spg025977 vs. ExPASy TrEMBL
Match: A0A6J1DDE8 (putative receptor-like protein kinase At1g72540 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111019749 PE=4 SV=1)

HSP 1 Score: 527.7 bits (1358), Expect = 3.6e-146
Identity = 264/329 (80.24%), Postives = 283/329 (86.02%), Query Frame = 0

Query: 10  AADSANGNVLVFTSEDLKAFTNNFNGHNLIGITQSGRFYRGQLRSSWSTATEAQNMTVKI 69
           +ADSAN  V VFTS+DLKAFT+NFNG NLIGIT SG  YRGQLRSSWSTA EA+N+TVKI
Sbjct: 41  SADSANMKVSVFTSQDLKAFTDNFNGKNLIGITHSGMLYRGQLRSSWSTAEEARNVTVKI 100

Query: 70  WDQNN----ECLVQEEVKLSTHPSLNGHPSLAKLIGYCCEDEVKGVVYDLNPLGSLQNLT 129
           WDQ      ECLV+EEVKL T PSL GHPS A L+GYCCEDEVKGVVYDLNPLG LQNL 
Sbjct: 101 WDQKPEFACECLVREEVKLLTQPSLKGHPSFANLVGYCCEDEVKGVVYDLNPLGFLQNLM 160

Query: 130 ARDELNWLQRVDVILELARLLDFIHSQEKQSLAFKFRASNILLDWECKPKLCGFGPVSNG 189
            RD  NWLQRVDVILELARLLDFIH QEK SLAF FRASNILLDWECKPKLCGF P SN 
Sbjct: 161 TRDNFNWLQRVDVILELARLLDFIHWQEKPSLAFDFRASNILLDWECKPKLCGFAPNSNE 220

Query: 190 MDTDLNKLYKFAQNPSAYVHPSTIRGGSQGEMSSDVYSLGEILLGLIAKREFEPEKLDKQ 249
             +DLNK YKFAQ PS Y HPS IRGGS+G+MSSDVYSLGEILLGLIAKR+ E EKL+KQ
Sbjct: 221 TVSDLNKPYKFAQIPSGYSHPS-IRGGSRGKMSSDVYSLGEILLGLIAKRDLEHEKLEKQ 280

Query: 250 SHHRVVNSLVSIWAKNEYRPNASLVHETLQKDWGYSAEEGVELTKLAMHSIEFFPRNRPS 309
            HH+VVNSLVSIWAKNEYRPNASLVHETLQ+DWGY+AEEGV +T+LAMH IEFFP NRPS
Sbjct: 281 DHHQVVNSLVSIWAKNEYRPNASLVHETLQEDWGYNAEEGVRITELAMHCIEFFPSNRPS 340

Query: 310 IKQILQRLEGLQVTQRLSDVRPRKKERKF 335
            KQ+LQ LEGLQV QRL+DVRPRKKERKF
Sbjct: 341 TKQVLQHLEGLQVAQRLTDVRPRKKERKF 368

BLAST of Spg025977 vs. ExPASy TrEMBL
Match: A0A6J1DEP7 (putative receptor-like protein kinase At1g72540 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111019749 PE=4 SV=1)

HSP 1 Score: 513.1 bits (1320), Expect = 9.1e-142
Identity = 265/360 (73.61%), Postives = 283/360 (78.61%), Query Frame = 0

Query: 10  AADSANGNVLVFTSEDLKAFTNNFNGHNLIGITQSGRFYRGQLRSSWSTATEAQNMTVKI 69
           +ADSAN  V VFTS+DLKAFT+NFNG NLIGIT SG  YRGQLRSSWSTA EA+N+TVKI
Sbjct: 41  SADSANMKVSVFTSQDLKAFTDNFNGKNLIGITHSGMLYRGQLRSSWSTAEEARNVTVKI 100

Query: 70  WDQNN----ECLV-------------------------------QEEVKLSTHPSLNGHP 129
           WDQ      ECLV                               QEEVKL T PSL GHP
Sbjct: 101 WDQKPEFACECLVRVSRQILSYTLHLQTQLAVFLSKLKICFTSSQEEVKLLTQPSLKGHP 160

Query: 130 SLAKLIGYCCEDEVKGVVYDLNPLGSLQNLTARDELNWLQRVDVILELARLLDFIHSQEK 189
           S A L+GYCCEDEVKGVVYDLNPLG LQNL  RD  NWLQRVDVILELARLLDFIH QEK
Sbjct: 161 SFANLVGYCCEDEVKGVVYDLNPLGFLQNLMTRDNFNWLQRVDVILELARLLDFIHWQEK 220

Query: 190 QSLAFKFRASNILLDWECKPKLCGFGPVSNGMDTDLNKLYKFAQNPSAYVHPSTIRGGSQ 249
            SLAF FRASNILLDWECKPKLCGF P SN   +DLNK YKFAQ PS Y HPS IRGGS+
Sbjct: 221 PSLAFDFRASNILLDWECKPKLCGFAPNSNETVSDLNKPYKFAQIPSGYSHPS-IRGGSR 280

Query: 250 GEMSSDVYSLGEILLGLIAKREFEPEKLDKQSHHRVVNSLVSIWAKNEYRPNASLVHETL 309
           G+MSSDVYSLGEILLGLIAKR+ E EKL+KQ HH+VVNSLVSIWAKNEYRPNASLVHETL
Sbjct: 281 GKMSSDVYSLGEILLGLIAKRDLEHEKLEKQDHHQVVNSLVSIWAKNEYRPNASLVHETL 340

Query: 310 QKDWGYSAEEGVELTKLAMHSIEFFPRNRPSIKQILQRLEGLQVTQRLSDVRPRKKERKF 335
           Q+DWGY+AEEGV +T+LAMH IEFFP NRPS KQ+LQ LEGLQV QRL+DVRPRKKERKF
Sbjct: 341 QEDWGYNAEEGVRITELAMHCIEFFPSNRPSTKQVLQHLEGLQVAQRLTDVRPRKKERKF 399

BLAST of Spg025977 vs. TAIR 10
Match: AT1G72540.1 (Protein kinase superfamily protein )

HSP 1 Score: 124.8 bits (312), Expect = 1.3e-28
Identity = 94/327 (28.75%), Postives = 157/327 (48.01%), Query Frame = 0

Query: 3   SFFNLHRAADSANGNVLVFTSEDLKAFTNNFNGHNLIGITQSGRFYRGQLRSSWSTATEA 62
           S  +L+  ++S   N+ +FT E+LK  T  F+ +N +G    G  Y+G +  S  T  + 
Sbjct: 54  STISLNDFSNSFFINIHIFTYEELKTITQGFSKYNFLGEGGFGEVYKGFVDDSLKTGLKD 113

Query: 63  QNMTVKIWDQNN---------ECLVQEEVKLSTHPSLNGHPSLAKLIGYCCEDEVKGVVY 122
           Q + VK   +           E ++  ++K         HP L  L+GYCCED+ + +VY
Sbjct: 114 QPVAVKALKREGGQGHREWLAEVIILGQLK---------HPHLVNLVGYCCEDDERLLVY 173

Query: 123 DLNPLGSLQNLTAR---DELNWLQRVDVILELARLLDFIHSQEKQSLAFKFRASNILLDW 182
           +    G+L++   +     L WL RV ++L  A+ L+F+H QEK  +   F+ SNILL  
Sbjct: 174 EYMERGNLEDHLFQKYGGALPWLTRVKILLGAAKGLEFLHKQEKPVIYRDFKPSNILLSS 233

Query: 183 ECKPKLCGFGPVSNGMDTDLNKLYKFAQNPSAYVHPSTIRGGSQGEMSSDVYSLGEILLG 242
           +   KL  FG  ++G + + +   K       Y  P  I  G+   M SDV+S G +LL 
Sbjct: 234 DFSSKLSDFGLATDGSEEEDSNFTKSVMGTEGYAAPEYISAGNLTTM-SDVFSFGVVLLE 293

Query: 243 LIAKREFEPEKLDKQSHHRVVNSLVSIWAKNEYR-PN--ASLVHETLQKDWGYSAEEGVE 302
           ++  R+   EK   Q    +V      WA+   + PN    ++  +L+    YS E   +
Sbjct: 294 MLTARK-AVEKYRAQRGRNLVE-----WARPMLKDPNKLERIIDPSLEGK--YSVEGIRK 353

Query: 303 LTKLAMHSIEFFPRNRPSIKQILQRLE 315
              LA   +   P++RP++  +++ LE
Sbjct: 354 AAALAYQCLSHNPKSRPTMTTVVKTLE 362

BLAST of Spg025977 vs. TAIR 10
Match: AT2G39660.1 (botrytis-induced kinase1 )

HSP 1 Score: 122.9 bits (307), Expect = 5.1e-28
Identity = 102/328 (31.10%), Postives = 153/328 (46.65%), Query Frame = 0

Query: 10  AADSANGNVL------VFTSEDLKAFTNNFNGHNLIGITQSGRFYRGQLRSSWSTATE-A 69
           AA    G +L       FT  +LK  T NF   ++IG    G  ++G L  S  T T+  
Sbjct: 38  AAQKTEGEILSSTPVKSFTFNELKLATRNFRPDSVIGEGGFGCVFKGWLDESTLTPTKPG 97

Query: 70  QNMTVKIWDQNNECLVQEEVKLSTHPSLN--GHPSLAKLIGYCCEDEVKGVVYDLNPLGS 129
             + + +   N E        L+    L    HP+L KLIGYC EDE + +VY+    GS
Sbjct: 98  TGLVIAVKKLNQEGFQGHREWLTEINYLGQLSHPNLVKLIGYCLEDEHRLLVYEFMQKGS 157

Query: 130 LQNLTAR-----DELNWLQRVDVILELARLLDFIHSQEKQSLAFKFRASNILLDWECKPK 189
           L+N   R       L W  RV+V L+ A+ L F+HS   + +    +ASNILLD +   K
Sbjct: 158 LENHLFRRGAYFKPLPWFLRVNVALDAAKGLAFLHSDPVKVIYRDIKASNILLDADYNAK 217

Query: 190 LCGFGPVSNGMDTDLNKLYKFAQNPSAYVHPSTIRGGSQGEMSSDVYSLGEILLGLIA-K 249
           L  FG   +G   DL+ +         Y  P  +  G      SDVYS G +LL +++ K
Sbjct: 218 LSDFGLARDGPMGDLSYVSTRVMGTYGYAAPEYMSSGHL-NARSDVYSFGVLLLEILSGK 277

Query: 250 REFEPEKLDKQSHHRVVNSLVSIWAKNEYRPNASLVHETL-----QKDWGYSAEEGVELT 309
           R  +  +  K+       +LV  WA    RP  +   + L     + D  Y  EE V + 
Sbjct: 278 RALDHNRPAKE------ENLVD-WA----RPYLTSKRKVLLIVDNRLDTQYLPEEAVRMA 337

Query: 310 KLAMHSIEFFPRNRPSIKQILQRLEGLQ 318
            +A+  + F P++RP++ Q+++ L+ LQ
Sbjct: 338 SVAVQCLSFEPKSRPTMDQVVRALQQLQ 353

BLAST of Spg025977 vs. TAIR 10
Match: AT5G35580.1 (Protein kinase superfamily protein )

HSP 1 Score: 120.6 bits (301), Expect = 2.5e-27
Identity = 93/313 (29.71%), Postives = 145/313 (46.33%), Query Frame = 0

Query: 14  ANGNVLVFTSEDLKAFTNNFNGHNLIGITQSGRFYRGQLRSSWSTATEAQNMTVKIWDQN 73
           A  ++ VFT  +L+  T +F+  N +G    G  ++G +        +AQ + VK+ D +
Sbjct: 57  AGSDLHVFTQAELRVITQSFSSSNFLGEGGFGPVHKGFIDDKLRPGLKAQPVAVKLLDLD 116

Query: 74  NECLVQEEVKLSTHPSLNG---HPSLAKLIGYCCEDEVKGVVYDLNPLGSLQNLTARD-- 133
               +Q   +  T     G   HP+L KLIGYCCE+  + +VY+  P GSL++   R   
Sbjct: 117 G---LQGHREFMTEVMCLGKLKHPNLVKLIGYCCEEAHRLLVYEFMPRGSLESQLFRRCS 176

Query: 134 -ELNWLQRVDVILELARLLDFIHSQEKQSLAFKFRASNILLDWECKPKLCGFGPVSNGMD 193
             L W  R+++  E A+ L F+H  EK  +   F+ASNILLD +   KL  FG   +G  
Sbjct: 177 LPLPWTTRLNIAYEAAKGLQFLHEAEKPIIYRDFKASNILLDSDYTAKLSDFGLAKDGPQ 236

Query: 194 TDLNKLYKFAQNPSAYVHPSTIRGGSQGEMSSDVYSLGEILLGLIAKREFEPEKLDKQSH 253
            D   +         Y  P  I  G      SDVYS G +LL L+  R    + +D    
Sbjct: 237 GDDTHVSTRVMGTQGYAAPEYIMTGHL-TAKSDVYSFGVVLLELLTGR----KSVDIARS 296

Query: 254 HRVVNSLVSIWAK---NEYRPNASLVHETLQKDWGYSAEEGVELTKLAMHSIEFFPRNRP 313
            R   +LV  WA+   N+ R    ++   L+    YS     +   LA   + + P+ RP
Sbjct: 297 SR-KETLVE-WARPMLNDARKLGRIMDPRLEDQ--YSETGARKAATLAYQCLRYRPKTRP 356

Query: 314 SIKQILQRLEGLQ 318
            I  ++  L+ ++
Sbjct: 357 DISTVVSVLQDIK 357

BLAST of Spg025977 vs. TAIR 10
Match: AT2G28930.1 (protein kinase 1B )

HSP 1 Score: 119.4 bits (298), Expect = 5.6e-27
Identity = 100/338 (29.59%), Postives = 156/338 (46.15%), Query Frame = 0

Query: 17  NVLVFTSEDLKAFTNNFNGHNLIGITQSGRFYRGQLRSSWSTATEAQN---MTVKIWDQN 76
           N+  FT  +LKA T NF   +++G    G  ++G +     TA++      + VK  +Q+
Sbjct: 64  NLKSFTFAELKAATRNFRPDSVLGEGGFGSVFKGWIDEQTLTASKPGTGVVIAVKKLNQD 123

Query: 77  NECLVQEEVKLSTHPSLNGHPSLAKLIGYCCEDEVKGVVYDLNPLGSLQNLTAR-----D 136
                QE +    +     HP+L KLIGYC EDE + +VY+  P GSL+N   R      
Sbjct: 124 GWQGHQEWLAEVNYLGQFSHPNLVKLIGYCLEDEHRLLVYEFMPRGSLENHLFRRGSYFQ 183

Query: 137 ELNWLQRVDVILELARLLDFIHSQEKQSLAFKFRASNILLDWECKPKLCGFGPVSNGMDT 196
            L+W  R+ V L  A+ L F+H+ E   +   F+ SNILLD E   KL  FG   +G   
Sbjct: 184 PLSWTLRLKVALGAAKGLAFLHNAETSVIYRDFKTSNILLDSEYNAKLSDFGLAKDGPTG 243

Query: 197 DLNKLYKFAQNPSAYVHPSTIRGGSQGEMSSDVYSLGEILLGLIAKREFEPEKLDKQSHH 256
           D + +         Y  P  +  G      SDVYS G +LL +++ R    +K       
Sbjct: 244 DKSHVSTRIMGTYGYAAPEYLATGHL-TTKSDVYSYGVVLLEVLSGRR-AVDKNRPPGEQ 303

Query: 257 RVVNSLVSIWAK---NEYRPNASLVHETLQKDWGYSAEEGVELTKLAMHSIEFFPRNRPS 316
           ++V      WA+      R    ++   LQ    YS EE  ++  LA+  + F  + RP+
Sbjct: 304 KLVE-----WARPLLANKRKLFRVIDNRLQDQ--YSMEEACKVATLALRCLTFEIKLRPN 363

Query: 317 IKQILQRLEGLQVTQ----RLSDVRPRKKERKFPSNAM 340
           + +++  LE +Q       R  D+  R+  R+  S A+
Sbjct: 364 MNEVVSHLEHIQTLNEAGGRNIDMVQRRMRRRSDSVAI 392

BLAST of Spg025977 vs. TAIR 10
Match: AT2G28930.2 (protein kinase 1B )

HSP 1 Score: 119.4 bits (298), Expect = 5.6e-27
Identity = 100/338 (29.59%), Postives = 156/338 (46.15%), Query Frame = 0

Query: 17  NVLVFTSEDLKAFTNNFNGHNLIGITQSGRFYRGQLRSSWSTATEAQN---MTVKIWDQN 76
           N+  FT  +LKA T NF   +++G    G  ++G +     TA++      + VK  +Q+
Sbjct: 53  NLKSFTFAELKAATRNFRPDSVLGEGGFGSVFKGWIDEQTLTASKPGTGVVIAVKKLNQD 112

Query: 77  NECLVQEEVKLSTHPSLNGHPSLAKLIGYCCEDEVKGVVYDLNPLGSLQNLTAR-----D 136
                QE +    +     HP+L KLIGYC EDE + +VY+  P GSL+N   R      
Sbjct: 113 GWQGHQEWLAEVNYLGQFSHPNLVKLIGYCLEDEHRLLVYEFMPRGSLENHLFRRGSYFQ 172

Query: 137 ELNWLQRVDVILELARLLDFIHSQEKQSLAFKFRASNILLDWECKPKLCGFGPVSNGMDT 196
            L+W  R+ V L  A+ L F+H+ E   +   F+ SNILLD E   KL  FG   +G   
Sbjct: 173 PLSWTLRLKVALGAAKGLAFLHNAETSVIYRDFKTSNILLDSEYNAKLSDFGLAKDGPTG 232

Query: 197 DLNKLYKFAQNPSAYVHPSTIRGGSQGEMSSDVYSLGEILLGLIAKREFEPEKLDKQSHH 256
           D + +         Y  P  +  G      SDVYS G +LL +++ R    +K       
Sbjct: 233 DKSHVSTRIMGTYGYAAPEYLATGHL-TTKSDVYSYGVVLLEVLSGRR-AVDKNRPPGEQ 292

Query: 257 RVVNSLVSIWAK---NEYRPNASLVHETLQKDWGYSAEEGVELTKLAMHSIEFFPRNRPS 316
           ++V      WA+      R    ++   LQ    YS EE  ++  LA+  + F  + RP+
Sbjct: 293 KLVE-----WARPLLANKRKLFRVIDNRLQDQ--YSMEEACKVATLALRCLTFEIKLRPN 352

Query: 317 IKQILQRLEGLQVTQ----RLSDVRPRKKERKFPSNAM 340
           + +++  LE +Q       R  D+  R+  R+  S A+
Sbjct: 353 MNEVVSHLEHIQTLNEAGGRNIDMVQRRMRRRSDSVAI 381

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022945250.11.2e-15681.38serine/threonine-protein kinase BIK1-like isoform X2 [Cucurbita moschata][more]
XP_022966807.11.2e-15680.52probable serine/threonine-protein kinase CST [Cucurbita maxima][more]
KAG6573595.13.9e-15580.80Serine/threonine-protein kinase PBL13, partial [Cucurbita argyrosperma subsp. so... [more]
XP_022945248.11.8e-15275.94putative receptor-like protein kinase At1g72540 isoform X1 [Cucurbita moschata][more]
XP_022151893.17.4e-14680.24putative receptor-like protein kinase At1g72540 isoform X2 [Momordica charantia][more]
Match NameE-valueIdentityDescription
Q9CAH11.9e-2728.75Putative receptor-like protein kinase At1g72540 OS=Arabidopsis thaliana OX=3702 ... [more]
O488147.2e-2731.10Serine/threonine-protein kinase BIK1 OS=Arabidopsis thaliana OX=3702 GN=BIK1 PE=... [more]
F4JZW13.6e-2629.71Serine/threonine-protein kinase PBL13 OS=Arabidopsis thaliana OX=3702 GN=PBL13 P... [more]
P465737.9e-2629.59Probable serine/threonine-protein kinase PBL10 OS=Arabidopsis thaliana OX=3702 G... [more]
Q9FM852.3e-2530.19Probable serine/threonine-protein kinase PBL16 OS=Arabidopsis thaliana OX=3702 G... [more]
Match NameE-valueIdentityDescription
A0A6J1HNY65.9e-15780.52probable serine/threonine-protein kinase CST OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A6J1G0E55.9e-15781.38serine/threonine-protein kinase BIK1-like isoform X2 OS=Cucurbita moschata OX=36... [more]
A0A6J1G0A18.8e-15375.94putative receptor-like protein kinase At1g72540 isoform X1 OS=Cucurbita moschata... [more]
A0A6J1DDE83.6e-14680.24putative receptor-like protein kinase At1g72540 isoform X2 OS=Momordica charanti... [more]
A0A6J1DEP79.1e-14273.61putative receptor-like protein kinase At1g72540 isoform X1 OS=Momordica charanti... [more]
Match NameE-valueIdentityDescription
AT1G72540.11.3e-2828.75Protein kinase superfamily protein [more]
AT2G39660.15.1e-2831.10botrytis-induced kinase1 [more]
AT5G35580.12.5e-2729.71Protein kinase superfamily protein [more]
AT2G28930.15.6e-2729.59protein kinase 1B [more]
AT2G28930.25.6e-2729.59protein kinase 1B [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 33..311
e-value: 0.0053
score: -94.2
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 70..311
e-value: 1.2E-12
score: 47.8
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 33..316
score: 19.241333
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 5..115
e-value: 5.8E-13
score: 50.5
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 117..332
e-value: 3.3E-23
score: 84.1
NoneNo IPR availablePANTHERPTHR45621:SF85RECEPTOR-LIKE KINASEcoord: 16..322
NoneNo IPR availablePANTHERPTHR45621OS01G0588500 PROTEIN-RELATEDcoord: 16..322
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 17..315

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg025977.1Spg025977.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0004672 protein kinase activity