Spg025524 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg025524
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Ribonuclease H
Location	scaffold13: 30727314 .. 30728833 (-)
RNA-Seq Expression	Spg025524
Synteny	Spg025524

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCAGGGAACCGAAGCCGAGGAGAAGAGGTTGACGCCAAAGTTACTGCCCTAGAGGATAAGGTGAGTGGAATGGTTTGAAGTTTGTCTAGGATACTTCGTATCTTGGATAAACCTGGTCCTAGCACCAAAATCCATGGGGAAGAATTGATTAGAGATCCAAGGAAGGGGAATGAGCCAATCGAATACGTTGAAGAGTCGGAGACAAGATTGAAGGGAAAGAAGACCAACAGCGCGACCAGCAAGGTCGGGGGGCTGAAACCTACGGAACGCACGATTTTGAGGAGCCCAGAACCAAGTATCAGTCGTAGAACAGACTTGAGGAACTTGATTGAGGAAAAGCACAGAATGGCCAAAGTTGCTGAGGTCGAGGCTAAGGCTGTCGAGGCCTAGGCTAGAGCTGCCAAGGCCTAGGTTAGGGCAGTCGAGGCCGAGGCTAGAGTGGCCGAGGCCGAGGCCAAGAGGGACAATCTCCCTTGGAAGACCGAGCTTCTAAACACCCTAAAGGAGCTCGGAAGTTCCCAAGGGGACCTGCAGAAATCAAAGGATTCTGGAGAACAAAACTTAGAAGAACTAATCGACCAGGTTGACCCATCCTTCACTGATGAAGTCATGAAAGCCGAGGTGTCCCAGAAGTTCAAGGTACCTACATTTAAACCGTATGATGGCAAGAAGGACCCCGTACAACACCTAGACGCCTACAGAAACTGGATGGACTTCCATGACGTTTCAGATGCAATCAGGTGTCGTGCTTTCTCTTTCACTCTAACAAGACCAGCCAAGCAATGTTTCAGACGTTGAAAAGAAGATCCATAAGCTCTTTCAAAGAATTAGCACGAGCATTCCTCTCGTAGTTCATGGGAGCCAGAGAACAATGCAAGCCTCACATCAACCTACTAATGTCAAACAACAGCCATGTGAGAGTTTGTGTGATTATATAACACGTTTTAACGAAGAAGCACTACAGGTTGAGGGGTACAGTGAATGAATAGCCCTAGTAGCCATAACAACAGGACTGGAAGACGAGAGATTGCTTAATTCAATATGTAAGAGCCAACCTCGAACTTATGTTGAATTTGTTTCCAGAGCCCAGAAGTACATGAGTGCAAAGGAGTTGCTTAGATCGAAGAGGTTAGAACACAAGAGGTATTCTTCATTCGACAGAGGACAGACGAGGTTGGTCGCGTCCGAGCCGACCAGACCGAAGAGACTACACAAGAAAGTTTGAAAAGTACACCCTTACCTCACTCCCACAGGAGAAAATCTTAATGGAAATTCGGAATACGAACCTCTTTAATGTACCAAGAAAGCTGAAGTCAAATCTTGACAAAAGAGACAAAGGTCAGTATTGTCTCTTCCATCGAGATCATGGGCATTCAACTAGGAACTGCAATCAACTGAAGGACGAAATTGAAGCACTAATTCGGAACAGGTACCTGAAGAAGTTCACCACTAAGGCCAAGGCGGTCAGAAATGGTCAAGGCAAAGATGATGATAGACCCCTTCTGGAAATCCGTTGA

mRNA sequence

ATGCCAGGGAACCGAAGCCGAGGAGAAGAGGTTGACGCCAAAGTTACTGCCCTAGAGGATAAGGTTAGGGCAGTCGAGGCCGAGGCTAGAGTGGCCGAGGCCGAGGCCAAGAGGGACAATCTCCCTTGGAAGACCGAGCTTCTAAACACCCTAAAGGAGCTCGGAAGTTCCCAAGGGGACCTGCAGAAATCAAAGGATTCTGGAGAACAAAACTTAGAAGAACTAATCGACCAGGTTGACCCATCCTTCACTGATGAAGTCATGAAAGCCGAGGTGTCCCAGAAGTTCAAGGTACCTACATTTAAACCGTATGATGGCAAGAAGGACCCCGTACAACACCTAGACGCCTACAGAAACTGGATGGACTTCCATGACGTTTCAGATGCAATCAGGTGTCAGGACAGACGAGGTTGGTCGCGTCCGAGCCGACCAGACCGAAGAGACTACACAAGAAAGTTTGAAAAGTACACCCTTACCTCACTCCCACAGGAGAAAATCTTAATGGAAATTCGGAATACGAACCTCTTTAATGTACCAAGAAAGCTGAAGTCAAATCTTGACAAAAGAGACAAAGGTCAGTATTGTCTCTTCCATCGAGATCATGGGCATTCAACTAGGAACTGCAATCAACTGAAGGACGAAATTGAAGCACTAATTCGGAACAGGTACCTGAAGAAGTTCACCACTAAGGCCAAGGCGGTCAGAAATGGTCAAGGCAAAGATGATGATAGACCCCTTCTGGAAATCCGTTGA

Coding sequence (CDS)

ATGCCAGGGAACCGAAGCCGAGGAGAAGAGGTTGACGCCAAAGTTACTGCCCTAGAGGATAAGGTTAGGGCAGTCGAGGCCGAGGCTAGAGTGGCCGAGGCCGAGGCCAAGAGGGACAATCTCCCTTGGAAGACCGAGCTTCTAAACACCCTAAAGGAGCTCGGAAGTTCCCAAGGGGACCTGCAGAAATCAAAGGATTCTGGAGAACAAAACTTAGAAGAACTAATCGACCAGGTTGACCCATCCTTCACTGATGAAGTCATGAAAGCCGAGGTGTCCCAGAAGTTCAAGGTACCTACATTTAAACCGTATGATGGCAAGAAGGACCCCGTACAACACCTAGACGCCTACAGAAACTGGATGGACTTCCATGACGTTTCAGATGCAATCAGGTGTCAGGACAGACGAGGTTGGTCGCGTCCGAGCCGACCAGACCGAAGAGACTACACAAGAAAGTTTGAAAAGTACACCCTTACCTCACTCCCACAGGAGAAAATCTTAATGGAAATTCGGAATACGAACCTCTTTAATGTACCAAGAAAGCTGAAGTCAAATCTTGACAAAAGAGACAAAGGTCAGTATTGTCTCTTCCATCGAGATCATGGGCATTCAACTAGGAACTGCAATCAACTGAAGGACGAAATTGAAGCACTAATTCGGAACAGGTACCTGAAGAAGTTCACCACTAAGGCCAAGGCGGTCAGAAATGGTCAAGGCAAAGATGATGATAGACCCCTTCTGGAAATCCGTTGA

Protein sequence

MPGNRSRGEEVDAKVTALEDKVRAVEAEARVAEAEAKRDNLPWKTELLNTLKELGSSQGDLQKSKDSGEQNLEELIDQVDPSFTDEVMKAEVSQKFKVPTFKPYDGKKDPVQHLDAYRNWMDFHDVSDAIRCQDRRGWSRPSRPDRRDYTRKFEKYTLTSLPQEKILMEIRNTNLFNVPRKLKSNLDKRDKGQYCLFHRDHGHSTRNCNQLKDEIEALIRNRYLKKFTTKAKAVRNGQGKDDDRPLLEIR

Homology

[Close]

BLAST of Spg025524 vs. NCBI nr
Match: XP_022158830.1 (uncharacterized protein LOC111025293 [Momordica charantia])

HSP 1 Score: 136.7 bits (343), Expect = 2.7e-28
Identity = 94/326 (28.83%), Postives = 130/326 (39.88%), Query Frame = 0


Query: 71  NLEELIDQVDPSFTDEVMKAEVSQKFKVPTFKPYDGKKDPVQHLDAYRNWMDFHDVSDAI 130
           +LEEL+DQ D  FT+E+M+ +V  KFK+PT K +D   DPV HLDAYR WMD + VS+A+
Sbjct: 168 DLEELLDQADSPFTEEIMREKVPPKFKLPTVKQFDXTTDPVDHLDAYREWMDIYGVSEAV 227

Query: 131 RC----------------QDRRG------------------------------------- 190
           RC                Q +RG                                     
Sbjct: 228 RCRVFSTTLNGSARIWFRQLKRGSISSFKSLARAFVTQFVGGRCRSRPVAYLLTIKQRTT 287

Query: 191 ------------------------------------------------------------ 250
                                                                       
Sbjct: 288 ESLRDYVARFNEEKLQVEGLTDAVSLLAFMSGVRDEHLSFSFGKRTPNTFSEALSRAQRY 347

[Close]

BLAST of Spg025524 vs. NCBI nr
Match: XP_022142767.1 (uncharacterized protein LOC111012805 [Momordica charantia])

HSP 1 Score: 110.2 bits (274), Expect = 2.7e-20
Identity = 52/113 (46.02%), Postives = 76/113 (67.26%), Query Frame = 0


Query: 138 WSRPSRPDRRDYTRKFEKYTLTSLPQEKILMEIRNTNLFNVPRKLKSNLDKRDKGQYCLF 197
           W +  R  ++D  +KFEKYT T++P E++LMEI+N  L   P ++ ++  KR KG+YCLF
Sbjct: 206 WEKRDRSCQKDPPQKFEKYTSTTVPLEQVLMEIKNQRLLKWPERMNASSTKRSKGRYCLF 265

Query: 198 HRDHGHSTRNCNQLKDEIEALIRNRYLKKFTTKAKAVRNGQGKDDDRPLLEIR 251
           H DHGH+T++C  LK+E+E LI   YLK++  + KA +NG+   D  P  EIR
Sbjct: 266 HWDHGHATQDCFDLKEEVEGLILRGYLKEYVEEPKATQNGE--SDKSPAREIR 316

[Close]

BLAST of Spg025524 vs. NCBI nr
Match: XP_022159368.1 (uncharacterized protein LOC111025785 [Momordica charantia])

HSP 1 Score: 106.3 bits (264), Expect = 3.9e-19
Identity = 51/113 (45.13%), Postives = 75/113 (66.37%), Query Frame = 0


Query: 138 WSRPSRPDRRDYTRKFEKYTLTSLPQEKILMEIRNTNLFNVPRKLKSNLDKRDKGQYCLF 197
           W +  R  ++D  RKFEKYT T++P E++LMEI++  L   P ++K    KR KG+YCLF
Sbjct: 165 WEKRDRSSQKDPPRKFEKYTPTTVPLEQVLMEIKDQRLLKWPERMKVPSTKRSKGRYCLF 224

Query: 198 HRDHGHSTRNCNQLKDEIEALIRNRYLKKFTTKAKAVRNGQGKDDDRPLLEIR 251
           HRDH H+T++   LK+E+E LIR  YL+++  + KA +NG+   +  P  EIR
Sbjct: 225 HRDHNHATQDYFDLKEEVEGLIRRGYLQEYVEEPKATQNGE--SEKSPAREIR 275

[Close]

BLAST of Spg025524 vs. NCBI nr
Match: XP_022159192.1 (uncharacterized protein LOC111025612 [Momordica charantia])

HSP 1 Score: 106.3 bits (264), Expect = 3.9e-19
Identity = 54/114 (47.37%), Postives = 75/114 (65.79%), Query Frame = 0


Query: 138 WSRPSR-PDRRDYTRKFEKYTLTSLPQEKILMEIRNTNLFNVPRKLKSNLDKRDKGQYCL 197
           W +  R   ++D  RKFEKYT T++P E++LMEI++  L   P  +K+  +KR KG+YCL
Sbjct: 65  WEKQDRVVGQKDLPRKFEKYTPTTVPLEQVLMEIKDQRLLKWPETMKAPPNKRSKGRYCL 124

Query: 198 FHRDHGHSTRNCNQLKDEIEALIRNRYLKKFTTKAKAVRNGQGKDDDRPLLEIR 251
           FHRDHGH+T +C  LK+E+E LIR  YLK++    KA  N +  D+  P  EIR
Sbjct: 125 FHRDHGHATLDCFDLKEEVEGLIRKGYLKEYVDDLKATPNRE-NDNKSPTREIR 177

[Close]

BLAST of Spg025524 vs. NCBI nr
Match: GFS37364.1 (hypothetical protein Acr_00g0051530 [Actinidia rufa])

HSP 1 Score: 99.4 bits (246), Expect = 4.8e-17
Identity = 83/293 (28.33%), Postives = 132/293 (45.05%), Query Frame = 0


Query: 2   PGNRSRGEEVDAKVTALEDKVRAVEAEARVAEAEAKRDNLPWKTELLNTLKELGSSQGDL 61
           P  R R    ++  T    +   V AEA +   E K  +          +++L  ++ D 
Sbjct: 62  PSIRERSSSSESSQTRSRVRRGEVPAEAIIPRRETKSTS--------QRIRDL-DARLDA 121

Query: 62  QKSKDSGEQNLEELIDQVDPSFTDEVMKAEVSQKFKVPT-FKPYDGKKDPVQHLDAYRNW 121
             +  +    ++ L+ Q DP FT  V+ A +S KFK+PT    Y+GK DP+ HLD+Y + 
Sbjct: 122 INTGTNAPVTVDTLVRQTDPLFTKRVLVARISSKFKLPTQLGIYEGKTDPMDHLDSYNSL 181

Query: 122 MDFHDVSDAIRCQ------------------------------------DRRGWSRPSRP 181
           M     SD + C+                                    D R  +R  RP
Sbjct: 182 MSLQGCSDEVMCKAFSATLKGPARGVSRGQTKRREKDDHKRKEPDTGRNDYREETRYKRP 241

Query: 182 DR---RDYTR------KFEKYTLTSL--PQEKILMEIRNTNLFNVPRKLKSNLDKRDKGQ 241
           DR   R   R      +  ++TL  L  P  ++L EI++      P K+K++  KR++ +
Sbjct: 242 DRDTKRSNNRRPRTPPRRPEFTLPPLNAPVAQVLSEIKHEEFVKWPGKIKTDPQKRNRNK 301

Query: 242 YCLFHRDHGHSTRNCNQLKDEIEALIRNRYLKKFTTKAKAVRNGQGK-DDDRP 246
           YC FHRDHGH+T +C QLK++I  LI+  YL+K+        + + +  D+RP
Sbjct: 302 YCEFHRDHGHNTEDCFQLKEQIADLIKRGYLRKYVAARPPPNSPERRYGDNRP 345

[Close]

BLAST of Spg025524 vs. ExPASy TrEMBL
Match: A0A6J1DWY0 (uncharacterized protein LOC111025293 OS=Momordica charantia OX=3673 GN=LOC111025293 PE=4 SV=1)

HSP 1 Score: 136.7 bits (343), Expect = 1.3e-28
Identity = 94/326 (28.83%), Postives = 130/326 (39.88%), Query Frame = 0


Query: 71  NLEELIDQVDPSFTDEVMKAEVSQKFKVPTFKPYDGKKDPVQHLDAYRNWMDFHDVSDAI 130
           +LEEL+DQ D  FT+E+M+ +V  KFK+PT K +D   DPV HLDAYR WMD + VS+A+
Sbjct: 168 DLEELLDQADSPFTEEIMREKVPPKFKLPTVKQFDXTTDPVDHLDAYREWMDIYGVSEAV 227

Query: 131 RC----------------QDRRG------------------------------------- 190
           RC                Q +RG                                     
Sbjct: 228 RCRVFSTTLNGSARIWFRQLKRGSISSFKSLARAFVTQFVGGRCRSRPVAYLLTIKQRTT 287

Query: 191 ------------------------------------------------------------ 250
                                                                       
Sbjct: 288 ESLRDYVARFNEEKLQVEGLTDAVSLLAFMSGVRDEHLSFSFGKRTPNTFSEALSRAQRY 347

[Close]

BLAST of Spg025524 vs. ExPASy TrEMBL
Match: A0A6J1CNT2 (uncharacterized protein LOC111012805 OS=Momordica charantia OX=3673 GN=LOC111012805 PE=4 SV=1)

HSP 1 Score: 110.2 bits (274), Expect = 1.3e-20
Identity = 52/113 (46.02%), Postives = 76/113 (67.26%), Query Frame = 0


Query: 138 WSRPSRPDRRDYTRKFEKYTLTSLPQEKILMEIRNTNLFNVPRKLKSNLDKRDKGQYCLF 197
           W +  R  ++D  +KFEKYT T++P E++LMEI+N  L   P ++ ++  KR KG+YCLF
Sbjct: 206 WEKRDRSCQKDPPQKFEKYTSTTVPLEQVLMEIKNQRLLKWPERMNASSTKRSKGRYCLF 265

Query: 198 HRDHGHSTRNCNQLKDEIEALIRNRYLKKFTTKAKAVRNGQGKDDDRPLLEIR 251
           H DHGH+T++C  LK+E+E LI   YLK++  + KA +NG+   D  P  EIR
Sbjct: 266 HWDHGHATQDCFDLKEEVEGLILRGYLKEYVEEPKATQNGE--SDKSPAREIR 316

[Close]

BLAST of Spg025524 vs. ExPASy TrEMBL
Match: A0A6J1DZ52 (uncharacterized protein LOC111025612 OS=Momordica charantia OX=3673 GN=LOC111025612 PE=4 SV=1)

HSP 1 Score: 106.3 bits (264), Expect = 1.9e-19
Identity = 54/114 (47.37%), Postives = 75/114 (65.79%), Query Frame = 0


Query: 138 WSRPSR-PDRRDYTRKFEKYTLTSLPQEKILMEIRNTNLFNVPRKLKSNLDKRDKGQYCL 197
           W +  R   ++D  RKFEKYT T++P E++LMEI++  L   P  +K+  +KR KG+YCL
Sbjct: 65  WEKQDRVVGQKDLPRKFEKYTPTTVPLEQVLMEIKDQRLLKWPETMKAPPNKRSKGRYCL 124

Query: 198 FHRDHGHSTRNCNQLKDEIEALIRNRYLKKFTTKAKAVRNGQGKDDDRPLLEIR 251
           FHRDHGH+T +C  LK+E+E LIR  YLK++    KA  N +  D+  P  EIR
Sbjct: 125 FHRDHGHATLDCFDLKEEVEGLIRKGYLKEYVDDLKATPNRE-NDNKSPTREIR 177

[Close]

BLAST of Spg025524 vs. ExPASy TrEMBL
Match: A0A6J1DYL6 (uncharacterized protein LOC111025785 OS=Momordica charantia OX=3673 GN=LOC111025785 PE=4 SV=1)

HSP 1 Score: 106.3 bits (264), Expect = 1.9e-19
Identity = 51/113 (45.13%), Postives = 75/113 (66.37%), Query Frame = 0


Query: 138 WSRPSRPDRRDYTRKFEKYTLTSLPQEKILMEIRNTNLFNVPRKLKSNLDKRDKGQYCLF 197
           W +  R  ++D  RKFEKYT T++P E++LMEI++  L   P ++K    KR KG+YCLF
Sbjct: 165 WEKRDRSSQKDPPRKFEKYTPTTVPLEQVLMEIKDQRLLKWPERMKVPSTKRSKGRYCLF 224

Query: 198 HRDHGHSTRNCNQLKDEIEALIRNRYLKKFTTKAKAVRNGQGKDDDRPLLEIR 251
           HRDH H+T++   LK+E+E LIR  YL+++  + KA +NG+   +  P  EIR
Sbjct: 225 HRDHNHATQDYFDLKEEVEGLIRRGYLQEYVEEPKATQNGE--SEKSPAREIR 275

[Close]

BLAST of Spg025524 vs. ExPASy TrEMBL
Match: A0A2N9J520 (Ribonuclease H OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS60449 PE=4 SV=1)

HSP 1 Score: 105.5 bits (262), Expect = 3.2e-19
Identity = 74/294 (25.17%), Postives = 131/294 (44.56%), Query Frame = 0


Query: 4   NRSRGEEVDAKVTALEDKVRAVEAEARVAEAEAKRDN---------LPWKTELLNTLKEL 63
           N+ RG + +++    +++ +  + + +  E E + +N           W+ E+     ++
Sbjct: 163 NKERG-DAESRQERQQEQQQEQQEQRQEQEGETRGENTRITVEQPSAKWEEEIKAMRAQM 222

Query: 64  GSSQGDLQKSKDSGEQNLEELIDQVDPSFTDEVMKAEVSQKFKVPTFKPYDGKKDPVQHL 123
           G  + +    K    +N+++L+   D  FT++V+   +  KF++P+ + +DG KDP+ HL
Sbjct: 223 GEMKDEF---KGRATKNIDDLVQGTDSPFTEKVISFPLPSKFRMPSLEIFDGSKDPLDHL 282

Query: 124 DAYRNWMDFHDVSDAIRCQ--------DRRGW-----------------------SRPSR 183
           ++++  M    V D I C+          R W                        RP+ 
Sbjct: 283 ESFKTVMCLQGVPDEIMCRAFPTTLKGPARIWFKKLMPGSLVIRPTGKKRKGEHADRPAE 342

Query: 184 PDR------------------RDYTRKFEKYTLTSLPQEKILMEIRNTNLFNVPRKLKSN 240
           P                    R +  +F ++T  + P + I M+IRN      P KL ++
Sbjct: 343 PQETRPKAQRNRNGRQEERSGRGFNERFNRFTPLNAPVDHIFMQIRNDPALKWPGKLLTD 402

The following BLAST results are available for this feature:

BLAST of Spg025524 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022158830.1	2.7e-28	28.83	uncharacterized protein LOC111025293 [Momordica charantia]	[more]
XP_022142767.1	2.7e-20	46.02	uncharacterized protein LOC111012805 [Momordica charantia]	[more]
XP_022159368.1	3.9e-19	45.13	uncharacterized protein LOC111025785 [Momordica charantia]	[more]
XP_022159192.1	3.9e-19	47.37	uncharacterized protein LOC111025612 [Momordica charantia]	[more]
GFS37364.1	4.8e-17	28.33	hypothetical protein Acr_00g0051530 [Actinidia rufa]	[more]

BLAST of Spg025524 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Spg025524 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DWY0	1.3e-28	28.83	uncharacterized protein LOC111025293 OS=Momordica charantia OX=3673 GN=LOC111025...	[more]
A0A6J1CNT2	1.3e-20	46.02	uncharacterized protein LOC111012805 OS=Momordica charantia OX=3673 GN=LOC111012...	[more]
A0A6J1DZ52	1.9e-19	47.37	uncharacterized protein LOC111025612 OS=Momordica charantia OX=3673 GN=LOC111025...	[more]
A0A6J1DYL6	1.9e-19	45.13	uncharacterized protein LOC111025785 OS=Momordica charantia OX=3673 GN=LOC111025...	[more]
A0A2N9J520	3.2e-19	25.17	Ribonuclease H OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS60449 PE=4 SV=1	[more]

BLAST of Spg025524 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 8..35

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg025524.1	Spg025524.1	mRNA