Spg025114 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg025114 vs. NCBI nr
Match: XP_020253860.1 (replication protein A 70 kDa DNA-binding subunit B-like [Asparagus officinalis])

HSP 1 Score: 141.0 bits (354), Expect = 1.8e-29
Identity = 88/277 (31.77%), Postives = 157/277 (56.68%), Query Frame = 0

Query: 45  GKPFRLTLWNDLATNEGLELEEQIIHNPIILAFNLKVNMYEGELSMATTMTSNILIEPDF 104
           G P R TL+ DLATNEG  LE +I + P+I   + K+++Y+G+ S+++ +   + I P  
Sbjct: 5   GMPMRATLFGDLATNEGSILEAEIKYKPVIALSDFKISIYQGDTSISSQIILTLCINPKI 64

Query: 105 VDSKDLKERRELLLDHTYMSTFIPPSKKMERAKLVSIKDIIH---GLVDDLYCCIIGSIV 164
             + +L++  +L        +   PS   + AK + I DI+H     +   YC    +I 
Sbjct: 65  ELASELRKWFQLEESAKAKGS---PSTLFKNAKEIHISDIMHKSQNSIQAQYCTFKVNIT 124

Query: 165 EIVNKDNPWFEKCKLCNQRVYPNGSQKMTHCNNCNDLVQF-IRKYMLKLKICDGQMTAHI 224
            I+N+ +PWF  CK C+++V  + S++  + + C  ++   I +Y++K+ + +G   A +
Sbjct: 125 TILNRLDPWFYACKGCDKKVDKDVSKECLN-DKCRRIIDDPIPRYLVKILVENGTDNARV 184

Query: 225 TAFEAAEVIVGSSVSHYATQILQMKKKEDSKIYRNFILAQGKTYKFLVRMDKKFEGDRSK 284
           T F+ AE ++G     + T+  + +K   S  Y+ F+L+ GKT+KFLV++DKK + +R +
Sbjct: 185 TLFDVAETLIGCKAETFITE--KKEKNNQSPYYQKFVLSIGKTFKFLVKLDKKTKDERGE 244

Query: 285 GHFVVQEIN-----KVEVEESDLETLEVLHVKKKIKI 313
           G+ + QEI       + V++ D  TLE+   KKKIK+
Sbjct: 245 GNIIAQEIQLQSDITINVDDED-ATLEL--PKKKIKL 272

BLAST of Spg025114 vs. NCBI nr
Match: KAG5560633.1 (hypothetical protein RHGRI_003829 [Rhododendron griersonianum])

HSP 1 Score: 138.3 bits (347), Expect = 1.2e-28
Identity = 96/303 (31.68%), Postives = 152/303 (50.17%), Query Frame = 0

Query: 25  KASRVGDFFPVYVAFVILECGKPFRLTLWNDLATNEGLELEEQIIHNPIILAFNLKVNMY 84
           KA  VG      V  +   C +   LTLW D+ATNEG  LE+     PI+    +K++ Y
Sbjct: 329 KAGGVGKSKKRRVTLINSSC-ESILLTLWGDMATNEGDMLEKMKTKRPIVALSKVKISTY 388

Query: 85  EGELSMATTMTSNILIEPDFVDSKDLKERRELLLDHTYMSTFIPPSKKMERAKLVSIKDI 144
            G LSM+T   + + ++P   ++  +K+R    L  T  +T     K  E A+ V I D+
Sbjct: 389 AGVLSMSTISITTLQVDPSIPETDAVKKRFNHNLQKTPGTT--SSGKNKETAERVKISDV 448

Query: 145 IHGLV---DDLYCCIIGSIVEIVNKDNPWFEKCKLCNQRVYPNGSQKMTHCNNCNDL-VQ 204
           +   +    + YC    +I+ I N + PW+  CK+CN++V P G Q M  C  C D   +
Sbjct: 449 MERSLLEFQNTYCSFKATIIRIDNMEQPWYNACKVCNKKVIP-GEQGM-ECTKCADKNSE 508

Query: 205 FIRKYMLKLKICDGQMTAHITAFEAAEVIVGSSVSHYATQILQMKKKEDS--KIYRNFIL 264
              +Y++K+ + DG+   +IT FEAAE I G SV       ++ K++E++  K+      
Sbjct: 509 HHPRYLMKITVGDGERQLYITLFEAAEKITGCSV----VDFIKSKEEENNQGKLIEQLRH 568

Query: 265 AQGKTYKFLVRMDKKFEGDRSKGHFVVQEINKVEVEESDLETLEVL-------HVKKKIK 315
           A GK Y F  ++DK  + DR+ G  +  E+      + D ET+E+          KK IK
Sbjct: 569 ASGKAYIFWAKLDKTNDKDRTLGKLIADEVQ----NDEDPETMEITSDVDEDNKTKKSIK 618

BLAST of Spg025114 vs. NCBI nr
Match: KAG5574299.1 (hypothetical protein H5410_054433 [Solanum commersonii])

HSP 1 Score: 136.7 bits (343), Expect = 3.4e-28
Identity = 88/269 (32.71%), Postives = 146/269 (54.28%), Query Frame = 0

Query: 50  LTLWNDLATNEGLELEEQIIHNPIILAFNLKVNMYEGELSMATTMTSNILIEPDFVDSKD 109
           +TLW D A N+G  LE+     PI+   +++V++++G   ++T   S++LI P F  + D
Sbjct: 155 VTLWGDFAENDGSFLEKLQDDKPILALCDVRVSIFKGRFGISTIPVSSVLINPMFQKAND 214

Query: 110 LKERRELLLDHTYMSTFIPPSKKMERAKLVSIKDIIHGLVDDLYCCII---GSIVEIVNK 169
           L+  RE++       T I P+K M+RA  V +  I+ G   D   C+     +I +I+NK
Sbjct: 215 LRAWREIIKADNKDLT-ITPTKVMKRAIEVPLAKILAGFSADSEDCMYKFKATIKDILNK 274

Query: 170 DNPWFEKCKLCNQRVYPNGSQKMTHCNNCN-DLVQFIRKYMLKLKICDGQMTAHITAFEA 229
           D PW+  CK C+++V     +    CNNCN D V++  +Y L+L++C+G+  A +  FEA
Sbjct: 275 DEPWYSACKKCHKKV--QFIEDTAACNNCNSDNVEYKMRYCLRLEVCNGERRARVILFEA 334

Query: 230 AEVIVGSSVSHYATQILQMKKKEDSKIYRNFILAQGKTYKFLVRMDKKFEGDRSKGHFVV 289
           A+ ++G +V  Y        KK++   YR  +L++   + FLVR+D      R     +V
Sbjct: 335 AKYLLGCNVQEYIES--NSVKKKECYYYRKLVLSKDNQFNFLVRIDMNDPNPRRS--LIV 394

Query: 290 QEINKVEVEESDL---ETLEVLHVKKKIK 312
           +EI++VE E   +   E   V   KK+ K
Sbjct: 395 EEIHQVEDEAPPIVEDEAKSVRIYKKRAK 416

BLAST of Spg025114 vs. NCBI nr
Match: XP_016485940.1 (PREDICTED: replication protein A 70 kDa DNA-binding subunit B-like [Nicotiana tabacum])

HSP 1 Score: 136.3 bits (342), Expect = 4.4e-28
Identity = 82/247 (33.20%), Postives = 134/247 (54.25%), Query Frame = 0

Query: 50  LTLWNDLATNEGLELEEQIIHNPIILAFNLKVNMYEGELSMATTMTSNILIEPDFVDSKD 109
           +TLWND +  +G  L++ I   P++ A +++   Y G  S++TT  S+I+I P F  +  
Sbjct: 180 VTLWNDFSEIDGQVLQKLIDEKPVVAACDVRKTSYRGNFSISTTYVSSIMINPKFEKAVA 239

Query: 110 LKERRELLLDHTYMSTFIPPSKKMERAKLVSIKDIIHGLVD---DLYCCIIGSIVEIVNK 169
           L++  +       +   + PS +++ A+ V I+DI     D   D Y      I +I+NK
Sbjct: 240 LQKWHDYKKAKN-IDISLLPSIQLKNAREVKIEDIKDDSFDNKEDTYFKFNARIKDIMNK 299

Query: 170 DNPWFEKCKLCNQRV-YPNGSQKMTHCNNCNDLVQFIRKYMLKLKICDGQMTAHITAFEA 229
           D PW+  CK C ++V   N   K  HC   N  V +  +Y+LKL++ D +   ++T FE+
Sbjct: 300 DEPWYSSCKKCYKKVDVINNIAKCHHCGVEN--VDYQERYILKLEVFDKKERCYVTLFES 359

Query: 230 AEVIVGSSVSHYATQILQMKKKEDSKIYRNFILAQGKTYKFLVRMDKKFEGDRSKGHFVV 289
              ++G  V+ Y   I Q  KKE+ K YR  +L++ K + FLV++D K  GDR    F+ 
Sbjct: 360 TRYLLGCDVTTYIQSISQ--KKEEFKFYRKLVLSREKEFTFLVKIDSKNGGDRDGRRFIA 419

Query: 290 QEINKVE 293
           +EI +VE
Sbjct: 420 EEIQEVE 421

BLAST of Spg025114 vs. NCBI nr
Match: KAG5563556.1 (hypothetical protein RHGRI_006105 [Rhododendron griersonianum])

HSP 1 Score: 136.0 bits (341), Expect = 5.8e-28
Identity = 95/303 (31.35%), Postives = 150/303 (49.50%), Query Frame = 0

Query: 25  KASRVGDFFPVYVAFVILECGKPFRLTLWNDLATNEGLELEEQIIHNPIILAFNLKVNMY 84
           KA  VG      V  +   C +   LTLW D+ATNEG  LE+     PI+    +K++ Y
Sbjct: 202 KAGGVGKSKKRSVTLINSSC-ESILLTLWGDMATNEGDMLEKMKTKRPIVALSKVKISTY 261

Query: 85  EGELSMATTMTSNILIEPDFVDSKDLKERRELLLDHTYMSTFIPPSKKMERAKLVSIKDI 144
            G LSM+T   + + ++P   ++  +K+R    L    M       K  E A+ V I D+
Sbjct: 262 AGVLSMSTISITTLQVDPSIPETDAVKKRFNHNLQK--MPGTTSSGKNKETAERVKISDV 321

Query: 145 IHGLV---DDLYCCIIGSIVEIVNKDNPWFEKCKLCNQRVYPNGSQKMTHCNNCNDL-VQ 204
           +   +    + YC    +I+ I N + PW+  CK+CN++V P G Q M  C  C D   +
Sbjct: 322 MERSLLEFQNTYCSFKATIIRIDNMEQPWYNACKVCNKKVIP-GEQGM-ECTKCADKNSE 381

Query: 205 FIRKYMLKLKICDGQMTAHITAFEAAEVIVGSSVSHYATQILQMKKKEDS--KIYRNFIL 264
              +Y++K+ + DG+   +IT FEAAE I G SV       ++ K++E++  K+      
Sbjct: 382 HHPRYLMKITVGDGERQLYITLFEAAEKITGCSV----VDFIKSKEEENNQGKLIEQLRH 441

Query: 265 AQGKTYKFLVRMDKKFEGDRSKGHFVVQEINKVEVEESDLETLEVL-------HVKKKIK 315
           A GK Y F  ++DK  + DR+ G  +  E+      + D ET+E+          KK IK
Sbjct: 442 ASGKAYIFWAKLDKTNDKDRTLGKLIADEVQ----NDEDPETMEITSDVDEDNKTKKSIK 491

BLAST of Spg025114 vs. ExPASy Swiss-Prot
Match: Q9FME0 (Replication protein A 70 kDa DNA-binding subunit D OS=Arabidopsis thaliana OX=3702 GN=RPA1D PE=2 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 1.3e-09
Identity = 56/221 (25.34%), Postives = 106/221 (47.96%), Query Frame = 0

Query: 43  ECGKPFRLTLWNDLATNEGLELEEQIIHNPIILAFNLKVNMYEGELSMATTMTSNILIEP 102
           E  K   ++LWNDLAT  G EL +    +P+I   +LKV  ++G +S++T   SN++I P
Sbjct: 351 ESRKTVVVSLWNDLATGIGQELLDMADQSPVIAIKSLKVGDFQG-VSLSTISRSNVVINP 410

Query: 103 DFVDSKDLK-----ERRELLLDHTYMSTFIPPSKKMERAKLVSIKDIIHGLVDD------ 162
           +  ++K LK     E +E+ +  + + + + PS K     L + + ++  +  +      
Sbjct: 411 ESPEAKKLKSWFDSEGKEISM--SSIGSGMSPSAKNGSRSLYTDRVLLSHITSNPSLFEE 470

Query: 163 --LYCCIIGSIVEIVNKDNPWFEKCKLCNQRVYPNGSQKMTHCNNC-NDLVQFIRKYMLK 222
             ++      I  I      W++ CK CN++V          C  C     +   +Y++ 
Sbjct: 471 KPVFFSTRAYISFIKPDQTMWYQACKTCNKKV-TEALDSGYWCEGCQRKYEECSLRYIMA 530

Query: 223 LKICDGQMTAHITAF-EAAEVIVGSSVSHYATQILQMKKKE 249
           +K+ D      I++F + AE I+G S    A ++ ++K +E
Sbjct: 531 VKVTDSSGETWISSFNDEAEKILGCS----ADELNKLKSEE 563

BLAST of Spg025114 vs. ExPASy Swiss-Prot
Match: Q10Q08 (Replication protein A 70 kDa DNA-binding subunit B OS=Oryza sativa subsp. japonica OX=39947 GN=RPA1B PE=1 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 2.8e-09
Identity = 60/225 (26.67%), Postives = 107/225 (47.56%), Query Frame = 0

Query: 43  ECGKPFRLTLWNDLATNEGLELEEQIIHNPIILAFNLKVNMYEGELSMATTMTSNILIEP 102
           +  K   ++LWNDLAT  G EL + +   PII   +LKV+ ++G LS++T   S I++ P
Sbjct: 355 DSSKTVTISLWNDLATTTGQELLDMVDSAPIIAIKSLKVSDFQG-LSLSTVGRSTIVVNP 414

Query: 103 DFVDSKDLKERRELLLDHTYMSTF--------IPPSKKM--ERAKLVSIKDIIHGLVDDL 162
           D  +++ L+   +     T M++         +  ++ M  +R  L  I    +   D  
Sbjct: 415 DLPEAEQLRAWYDSEGKGTSMASIGSDMGASRVGGARSMYSDRVFLSHITSDPNLGQDKP 474

Query: 163 YCCIIGSIVEIVNKDNP-WFEKCKLCNQRVYPNGSQKMTHCNNC--NDLVQFIRKYMLKL 222
               + + + ++  D   W+  CK CN++V          C  C  ND    +R Y++ +
Sbjct: 475 VFFSLNAYISLIKPDQTMWYRACKTCNKKV-TEAIGSGYWCEGCQKNDAECSLR-YIMVI 534

Query: 223 KICDGQMTAHITAF-EAAEVIVGSSVSHYATQILQMKKKEDSKIY 254
           K+ D    A ++ F + AE IVG S    A ++ +++K+E    Y
Sbjct: 535 KVSDPTGEAWLSLFNDQAERIVGCS----ADELDRIRKEEGDDSY 572

BLAST of Spg025114 vs. ExPASy Swiss-Prot
Match: Q9SD82 (Replication protein A 70 kDa DNA-binding subunit B OS=Arabidopsis thaliana OX=3702 GN=RPA1B PE=3 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 5.3e-08
Identity = 51/202 (25.25%), Postives = 90/202 (44.55%), Query Frame = 0

Query: 43  ECGKPFRLTLWNDLATNEGLELEEQIIHNPIILAFNLKVNMYEGELSMATTMTSNILIEP 102
           E  K   ++LWNDLAT  G EL +   ++P+I   +LKV  ++G +S++T   SN++I P
Sbjct: 327 ETKKTVVVSLWNDLATGIGQELLDMADNHPVIAIKSLKVGAFQG-VSLSTISRSNVVINP 386

Query: 103 DFVDSKDLKERRELLLDHTYMSTF---IPPSKKMERAKLVSIKDIIHGLVDD-------- 162
           +  ++  LK   +     T MS     +  S       + S +  +  +  +        
Sbjct: 387 NSPEATKLKSWYDAEGKETSMSAIGSGMSSSANNGSRSMYSDRVFLSHITSNPSLGEEKP 446

Query: 163 LYCCIIGSIVEIVNKDNPWFEKCKLCNQRVYPNGSQKMTHCNNCNDLVQFIR-KYMLKLK 222
           ++      I  I      W+  CK CN++V          C +C    Q    +Y++ +K
Sbjct: 447 VFFSTRAYISFIKPDQTMWYRACKTCNKKV-TEAMDSGYWCESCQKKDQECSLRYIMAVK 506

Query: 223 ICDGQMTAHITAF-EAAEVIVG 232
           + D      ++AF + AE I+G
Sbjct: 507 VSDSTGETWLSAFNDEAEKIIG 526

BLAST of Spg025114 vs. ExPASy TrEMBL
Match: A0A1S4BAI4 (replication protein A 70 kDa DNA-binding subunit B-like OS=Nicotiana tabacum OX=4097 GN=LOC107806315 PE=4 SV=1)

HSP 1 Score: 136.3 bits (342), Expect = 2.1e-28
Identity = 82/247 (33.20%), Postives = 134/247 (54.25%), Query Frame = 0

Query: 50  LTLWNDLATNEGLELEEQIIHNPIILAFNLKVNMYEGELSMATTMTSNILIEPDFVDSKD 109
           +TLWND +  +G  L++ I   P++ A +++   Y G  S++TT  S+I+I P F  +  
Sbjct: 180 VTLWNDFSEIDGQVLQKLIDEKPVVAACDVRKTSYRGNFSISTTYVSSIMINPKFEKAVA 239

Query: 110 LKERRELLLDHTYMSTFIPPSKKMERAKLVSIKDIIHGLVD---DLYCCIIGSIVEIVNK 169
           L++  +       +   + PS +++ A+ V I+DI     D   D Y      I +I+NK
Sbjct: 240 LQKWHDYKKAKN-IDISLLPSIQLKNAREVKIEDIKDDSFDNKEDTYFKFNARIKDIMNK 299

Query: 170 DNPWFEKCKLCNQRV-YPNGSQKMTHCNNCNDLVQFIRKYMLKLKICDGQMTAHITAFEA 229
           D PW+  CK C ++V   N   K  HC   N  V +  +Y+LKL++ D +   ++T FE+
Sbjct: 300 DEPWYSSCKKCYKKVDVINNIAKCHHCGVEN--VDYQERYILKLEVFDKKERCYVTLFES 359

Query: 230 AEVIVGSSVSHYATQILQMKKKEDSKIYRNFILAQGKTYKFLVRMDKKFEGDRSKGHFVV 289
              ++G  V+ Y   I Q  KKE+ K YR  +L++ K + FLV++D K  GDR    F+ 
Sbjct: 360 TRYLLGCDVTTYIQSISQ--KKEEFKFYRKLVLSREKEFTFLVKIDSKNGGDRDGRRFIA 419

Query: 290 QEINKVE 293
           +EI +VE
Sbjct: 420 EEIQEVE 421

BLAST of Spg025114 vs. ExPASy TrEMBL
Match: M1DXI7 (Replication protein A1 OS=Solanum tuberosum OX=4113 PE=4 SV=1)

HSP 1 Score: 136.0 bits (341), Expect = 2.8e-28
Identity = 86/271 (31.73%), Postives = 147/271 (54.24%), Query Frame = 0

Query: 43  ECGKPFRLTLWNDLATNEGLELEEQIIHNPIILAFNLKVNMYEGELSMATTMTSNILIEP 102
           E  +P  +TLW D A N+G  L++     PI+   +++V++Y+G   ++T   S++LI P
Sbjct: 173 ELMEPTTVTLWGDFAENDGAFLDKLKDDKPILGLCDVRVSIYKGRFGISTIPVSSVLINP 232

Query: 103 DFVDSKDLKERRELLLDHTYMSTFIPPSKKMERAKLVSIKDIIHGLV----DDLYCCIIG 162
            F  + DL+  RE +++       + P+K M+RA  V +  I++GL+    D +Y     
Sbjct: 233 IFQKALDLRAWRE-IIEADNKDIIVAPTKAMKRAIEVPLDHILNGLLAESQDSMY-KFKA 292

Query: 163 SIVEIVNKDNPWFEKCKLCNQRVYPNGSQKMTHCNNCN-DLVQFIRKYMLKLKICDGQMT 222
           +IV+I+NKD PW+  CK C ++V     ++   C NCN D V++  +Y L+L++C G+  
Sbjct: 293 TIVDILNKDEPWYLSCKTCRKKV--KVIKEAAACTNCNIDNVEYAMRYCLRLEVCGGEKR 352

Query: 223 AHITAFEAAEVIVGSSVSHYATQILQMKKKEDSKIYRNFILAQGKTYKFLVRMDKKFEGD 282
           A +  FEAA  ++G              KKE+   YRN +L++ K + FLVR+D      
Sbjct: 353 AQVVLFEAANNLLGC-------------KKEECHYYRNLVLSKDKQFNFLVRIDLTDANP 412

Query: 283 RSKGHFVVQEINKVEVEESDLETLEVLHVKK 309
           R     + +EI++++ E   +   EV  V+K
Sbjct: 413 RRS--LIAEEIHQLKDEAPIILEEEVRSVRK 424

BLAST of Spg025114 vs. ExPASy TrEMBL
Match: A0A1J6KWZ0 (Rep_fac-A_C domain-containing protein OS=Nicotiana attenuata OX=49451 GN=A4A49_29609 PE=4 SV=1)

HSP 1 Score: 132.1 bits (331), Expect = 4.0e-27
Identity = 83/276 (30.07%), Postives = 144/276 (52.17%), Query Frame = 0

Query: 50  LTLWNDLATNEGLELEEQIIHNPIILAFNLKVNMYEGELSMATTMTSNILIEPDFVDSKD 109
           +TLW ++A  +G  L+  I   PI++  N+K   Y G   ++TT  S+++I P F  +  
Sbjct: 141 VTLWGEIAEKDGDMLQNMIFEKPIVVLCNVKGTTYRGNFVISTTHVSSVMINPSFEKAIA 200

Query: 110 LK--ERRELLLDHTYMSTFIPPSKKMERAKLVSIKDIIHG----LVDDLYCCIIGSIVEI 169
           L+    R  L D       + PS+ ++ A+ V I DI H        D Y      I  I
Sbjct: 201 LQNWHNRMKLQD---KDITLMPSRLIKNARKVKIGDITHADSFDNTKDTYRKFNTKIKYI 260

Query: 170 VNKDNPWFEKCKLCNQRVYPNGSQKMTHCNNCNDLVQFIRKYMLKLKICDGQMTAHITAF 229
           +NKD+PW+  C  C ++V  +    +  C  C   V++  +Y+LKL++ D +   +IT F
Sbjct: 261 INKDDPWYLSCNKCYKKV--SLVDNIATCPRCGSNVEYEERYLLKLELFDEKEHCYITLF 320

Query: 230 EAAEVIVGSSVSHYATQILQMKKKEDSKIYRNFILAQGKTYKFLVRMDKKFEGDRSKGHF 289
           EA + ++G  V  Y   I Q  KKE+SK YR  + ++ K + FLV++D +  GDR+    
Sbjct: 321 EATKYLLGCDVKTYVRSISQ--KKEESKYYRKLVSSKDKEFTFLVKLDPRIGGDRTGRSL 380

Query: 290 VVQEINKV------EVEESDLETLEVLHVKKKIKIE 314
           + +E+++V      E+E+ +++  + +   K+I+ E
Sbjct: 381 IAEELHEVGKVDAIEIEDDEIKAAKTIKKIKQIEEE 409

BLAST of Spg025114 vs. ExPASy TrEMBL
Match: M1DP79 (Replication factor A OS=Solanum tuberosum OX=4113 PE=4 SV=1)

HSP 1 Score: 131.3 bits (329), Expect = 6.9e-27
Identity = 89/291 (30.58%), Postives = 150/291 (51.55%), Query Frame = 0

Query: 35  VYVAFVILECGKPFRLTLWNDLATNEGLELEEQIIHNPIILAFNLKVNMYEGELSMATTM 94
           + V   ++EC     +TLW D A N+G  LE+     PI+   +++V++Y+G   ++T  
Sbjct: 143 IVVTNELMEC---TTVTLWGDFAENDGAFLEKLKDDQPILGLCDVRVSIYKGTFGISTIP 202

Query: 95  TSNILIEPDFVDSKDLKERRELLLDHTYMSTFIPPSKKMERAKLVSIKDIIHGLVDDLYC 154
            S++LI P F  + DL+  RE++       T   PSK M +A  V++ +I+ G + D   
Sbjct: 203 VSSVLINPIFQKALDLRAWREVIKAEKKPIT-TTPSKVMRKATEVTLLNIMDGTLADTQE 262

Query: 155 CII---GSIVEIVNKDNPWFEKCKLCNQRVYPNGSQKMTHCNNCN-DLVQFIRKYMLKLK 214
            +      IV+I+N + PW+  CK C+++V     Q    C NCN    ++  +Y L+L+
Sbjct: 263 ALYKFKAKIVDILNIEEPWYSSCKQCHKKV--QVKQDTVTCPNCNIQNGEYEMRYSLRLE 322

Query: 215 ICDGQMTAHITAFEAAEVIVGSSVSHYATQILQMKKKEDSKIYRNFILAQGKTYKFLVRM 274
           +CD +  A +T F+AA++++G +V  Y       +K E+S  YRN + ++ K +KFLVR+
Sbjct: 323 VCDEEQRARVTLFDAAQMLLGCTVEDYIK--TTSEKNEESMYYRNLVFSKDKEFKFLVRI 382

Query: 275 DKKFEGDRSKGHFVVQEINKVE-------VEESDLETLEVLHVKKKIKIER 315
           D            + QEI+ VE        +ES  +T       K   +ER
Sbjct: 383 D--MNNSNPCRSLIAQEIHPVEDEAVILMADESKSQTPPTNRANKSNALER 423

BLAST of Spg025114 vs. ExPASy TrEMBL
Match: A0A1S4BU38 (replication protein A 70 kDa DNA-binding subunit B-like OS=Nicotiana tabacum OX=4097 GN=LOC107811908 PE=4 SV=1)

HSP 1 Score: 128.6 bits (322), Expect = 4.4e-26
Identity = 81/272 (29.78%), Postives = 141/272 (51.84%), Query Frame = 0

Query: 50  LTLWNDLATNEGLELEEQIIHNPIILAFNLKVNMYEGELSMATTMTSNILIEPDFVDSKD 109
           +TLW ++A  +G  L+  I   PI+   ++K   Y G   ++TT  S+++  P F  +  
Sbjct: 120 VTLWGEVAEKDGDILQNMIFEKPIVALCDVKGITYRGNFVISTTHVSSVMTNPSFEKAIA 179

Query: 110 LKERRELLLDHTYMSTFIPPSKKMERAKLVSIKDIIHG----LVDDLYCCIIGSIVEIVN 169
           L+     +       T + PS+ +++A+ V I DI H       +D Y      I +I+N
Sbjct: 180 LQNWHNCMKSQDKDITLM-PSRLIKKARKVKIGDITHADSFDNTEDTYRKFNVKIKDIIN 239

Query: 170 KDNPWFEKCKLCNQRVYPNGSQKMTHCNNCNDLVQFIRKYMLKLKICDGQMTAHITAFEA 229
            D+PW+  CK C ++V  +    +  C  C   V++  +Y+LKL++ D +   +IT FEA
Sbjct: 240 NDDPWYLSCKKCYKKV--SLVDNIATCPRCGSNVEYEERYLLKLELFDEKEHCYITLFEA 299

Query: 230 AEVIVGSSVSHYATQILQMKKKEDSKIYRNFILAQGKTYKFLVRMDKKFEGDRSKGHFVV 289
            + ++G  V+ Y   I Q  KKE+SK YR  + ++ K + FLV++D +  GDR     + 
Sbjct: 300 TKYLLGCDVTTYVRSISQ--KKEESKYYRKLVSSKDKEFTFLVKLDPRIGGDRPGKSLIA 359

Query: 290 QEINKV----EVEESDLETLEVLHVKKKIKIE 314
           +E+++V     +E +D E      +KK  +IE
Sbjct: 360 EELHEVGKVDAIEIADDEIKAAKTIKKTKQIE 386

BLAST of Spg025114 vs. TAIR 10
Match: AT5G61000.1 (Replication factor-A protein 1-related )

HSP 1 Score: 65.5 bits (158), Expect = 8.9e-11
Identity = 56/221 (25.34%), Postives = 106/221 (47.96%), Query Frame = 0

Query: 43  ECGKPFRLTLWNDLATNEGLELEEQIIHNPIILAFNLKVNMYEGELSMATTMTSNILIEP 102
           E  K   ++LWNDLAT  G EL +    +P+I   +LKV  ++G +S++T   SN++I P
Sbjct: 351 ESRKTVVVSLWNDLATGIGQELLDMADQSPVIAIKSLKVGDFQG-VSLSTISRSNVVINP 410

Query: 103 DFVDSKDLK-----ERRELLLDHTYMSTFIPPSKKMERAKLVSIKDIIHGLVDD------ 162
           +  ++K LK     E +E+ +  + + + + PS K     L + + ++  +  +      
Sbjct: 411 ESPEAKKLKSWFDSEGKEISM--SSIGSGMSPSAKNGSRSLYTDRVLLSHITSNPSLFEE 470

Query: 163 --LYCCIIGSIVEIVNKDNPWFEKCKLCNQRVYPNGSQKMTHCNNC-NDLVQFIRKYMLK 222
             ++      I  I      W++ CK CN++V          C  C     +   +Y++ 
Sbjct: 471 KPVFFSTRAYISFIKPDQTMWYQACKTCNKKV-TEALDSGYWCEGCQRKYEECSLRYIMA 530

Query: 223 LKICDGQMTAHITAF-EAAEVIVGSSVSHYATQILQMKKKE 249
           +K+ D      I++F + AE I+G S    A ++ ++K +E
Sbjct: 531 VKVTDSSGETWISSFNDEAEKILGCS----ADELNKLKSEE 563

BLAST of Spg025114 vs. TAIR 10
Match: AT5G08020.1 (RPA70-kDa subunit B )

HSP 1 Score: 60.1 bits (144), Expect = 3.7e-09
Identity = 51/202 (25.25%), Postives = 90/202 (44.55%), Query Frame = 0

Query: 43  ECGKPFRLTLWNDLATNEGLELEEQIIHNPIILAFNLKVNMYEGELSMATTMTSNILIEP 102
           E  K   ++LWNDLAT  G EL +   ++P+I   +LKV  ++G +S++T   SN++I P
Sbjct: 327 ETKKTVVVSLWNDLATGIGQELLDMADNHPVIAIKSLKVGAFQG-VSLSTISRSNVVINP 386

Query: 103 DFVDSKDLKERRELLLDHTYMSTF---IPPSKKMERAKLVSIKDIIHGLVDD-------- 162
           +  ++  LK   +     T MS     +  S       + S +  +  +  +        
Sbjct: 387 NSPEATKLKSWYDAEGKETSMSAIGSGMSSSANNGSRSMYSDRVFLSHITSNPSLGEEKP 446

Query: 163 LYCCIIGSIVEIVNKDNPWFEKCKLCNQRVYPNGSQKMTHCNNCNDLVQFIR-KYMLKLK 222
           ++      I  I      W+  CK CN++V          C +C    Q    +Y++ +K
Sbjct: 447 VFFSTRAYISFIKPDQTMWYRACKTCNKKV-TEAMDSGYWCESCQKKDQECSLRYIMAVK 506

Query: 223 ICDGQMTAHITAF-EAAEVIVG 232
           + D      ++AF + AE I+G
Sbjct: 507 VSDSTGETWLSAFNDEAEKIIG 526