Spg024955 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg024955
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	tRNA pseudouridine synthase
Location	scaffold12: 13747905 .. 13750293 (+)
RNA-Seq Expression	Spg024955
Synteny	Spg024955

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AACACATACCTAGAAGAAGGATGAGGTGCTGGATAGTGGGTTCGGCGGTTCTTCCTCCAATTCCCCTTCCCCTTTCACCAACCATCCCATCTCCAAATGGCTCTTCATCTTCCACCAATCGTAGATTCCCGGTAATGCCCATTCTTCTCTGATCCTTCCTCTCCTCTACTCGTATTTCTGGTTTAATTTTGATGATGTTTCTGTGGTCGTTGCCTTGCCGCGATTCTTCCAAAGAATGCAGAGGATTCCTGTTATGTCACAGAGCCCCCTTTCCCTAGCTTTAAATGGCGGTTGGTCATTGCTTATGACGGTACGCGATATGCAGGTCCGGCTTTCTTTTTCTTCTCTCCGATTGTTTATTCAGGCGAATGAATTAAACGATTTTGCATCCATATACCAGCTTTATCTTTTTAGGTTAATGGTAATTCTAATCGGCTTAAGCTTTTGAGGTTAGTTGCTGTTGGATGTCTTTTTCATCATCAGTATCATTTCGCCGCAAGATCACCCAAATTTGAGACTCGTATAAAAGTACTTGCCTACTTTTATTTCGTTTTGATCATGATTGTTTTTACTTGTTTTAAGTTATCATAGAGAGACATGATTTGGGTTCCGGTGTGAATTATGAGTTAACTCAGATATTGCATTCCTGCGCTTACTACTACAATTTTGAGGCTCTTTTCGTCTTTCTCCAAAAGTTTGATAAAATAACTTTAAATTTGGTGCTACTAAATTAGGCTGGCAATATCAGCGGTCACCTCCTACCATCCAGTGCCTGATTGAAAATGCCTTGACTCGAGCAACAAAGCTTGAGAGGAAGGATCTCCGTTTAGTTGGTGCAAGCAGAACAGATACGGGAGTTCATGCCTGGGGTCAGGTCTGATGTGCTTTTTGGAGTTGGGTTTAACACATCCTTGGAAAACAATGCTTGCTGTTGCTTACAACAAGTTGAGGTTTCAGGTGGCACACTTTATTACGCCATTTAACTATGATAGCTTGGATAACGTTCATGCTGCTTTGAATGGTCTTCTTCCTGCGGATATAAGAGTTCGGGAGATTAGTCCTGCTGTGCCTGACTTTCATGCTCGGTTTTCTACAATAAGCAAGGTTTATCACTACAAGATATATAATGGCACCATCTTGGATCCATTTCAGCGGTGCTACACTTACCATTGCGTTTATAAACTCAATTATGCAGCCATGAGAGAAGCTGCAAATTATTTCATTGGAAAGCATGATTTTTCTGCTTTTGCAAATGCAGCACATAATGATCGAGTGGTAGATCCAGTGAAGGTCATTTTTCGTTTTGACGTCATAGAAATGGTAAAATTTTAAGGTTCTCTAGTCCAGTGATGTTTTTTCTATAATGTGTCTGTTTCTCATGAGTCTAGCGTGGTTAGTTCTGTATCCAACTCTTTTGGTTATCTGTGTGAATCATTCTATTTTCTGCTTTATTCAAAAACTGAGGTTAAATGCTTAGCATGTGATTCTTTGTTCAGGGACCTCTTGTTCAGCTAGAAGTTGAGGGTTCTGGGTTCTTGTATAGACAAGTTCGGAATATGGTAATTATATTTGTTACACCCATTTTTTTAGTCGATTTCCCTTGTCCGATATTTTACTTAGTAAATGCTGGTCACTATAATAGTCTTATAGTATCTTTCATTTTTTCTCAATGAAAGCATGGTCTTTTATCAAAAAAATAATAGTTTTATAGTAGGCCAATTTGAGCCTAGCTCAACTGGTGAAGATATTCTCGACCAAAAGGTCAGAGATTCGAGTCTTTCTTCCTATGTGTTTTGAACTAAAAAAAAGACTTTAGTAGAAAGTAAACTTCCTGGAAGCGTAGATTTAAGTTTGTAAAATCTGGTTCATGCAACTGTAAGCATTTCTCTGGTACTTTTGCAGGTTGCCTTGTTGATTCAAATCGGAAAGGAAGCAATTGCTCCTGATGTTGTTCCTAAGATATTGGCCTCTGGAGATCGCAAGGAGCTTGCCAAGTATGCTCCGTGCATTCCTCCGCAGGGGCTCTGTCTTATGAGTATTAATTATAAAGAGGACCATCTAAAGCTTCCATCTGGTTGCCCTGGGACTAGTTTTGGTAGGCGTCATAGTGTGAGCAAATGTAAGCTCCCTTTCTATTGAATTAATGACGGATCTGTTCTTATGAGCATAATTTTCATTGCCGGTTCCAACAAATTAGAGGATGAGATATTAGATAATTAGTGTAGTTTAGTTAACATTAGTTTAGCTAACTTTGGATTTAGTTTTTATTGATTTGTGAAGTAGTATTTGTGTGTGATTGTTATTTGTCTTTTTTCTTGTTAGGCCTATTATATTAAGTACAGAAATTATAGCGAGTGGGTTCAATTAACCTATTAATTACATGTTTGTC

mRNA sequence

ATGGCTCTTCATCTTCCACCAATCGTAGATTCCCGAATGCAGAGGATTCCTGTTATGTCACAGAGCCCCCTTTCCCTAGCTTTAAATGGCGGTTGGTCATTGCTTATGACGGTACGCGATATGCAGGTTAATGGTAATTCTAATCGGCTTAAGCTTTTGAGACATGATTTGGGTTCCGGTGTGAATTATGAGTTAACTCAGATATTGCATTCCTGCGCTTACTACTACAATTTTGAGGCTCTTTTCTGCCTGATTGAAAATGCCTTGACTCGAGCAACAAAGCTTGAGAGGAAGGATCTCCGTTTAGTTGGTGCAAGCAGAACAGATACGGGAGTTCATGCCTGGGGTCAGGTGGCACACTTTATTACGCCATTTAACTATGATAGCTTGGATAACGTTCATGCTGCTTTGAATGGTCTTCTTCCTGCGGATATAAGAGTTCGGGAGATTAGTCCTGCTGTGCCTGACTTTCATGCTCGGTTTTCTACAATAAGCAAGGTTTATCACTACAAGATATATAATGGCACCATCTTGGATCCATTTCAGCGGTGCTACACTTACCATTGCGTTTATAAACTCAATTATGCAGCCATGAGAGAAGCTGCAAATTATTTCATTGGAAAGCATGATTTTTCTGCTTTTGCAAATGCAGCACATAATGATCGAGTGGTAGATCCAGTGAAGGTCATTTTTCGTTTTGACGTCATAGAAATGGGACCTCTTGTTCAGCTAGAAGTTGAGGGTTCTGGGTTCTTGTATAGACAAGTTCGGAATATGGTTGCCTTGTTGATTCAAATCGGAAAGGAAGCAATTGCTCCTGATGTTGTTCCTAAGATATTGGCCTCTGGAGATCGCAAGGAGCTTGCCAAGTATGCTCCGTGCATTCCTCCGCAGGGGCTCTGTCTTATGAGTATTAATTATAAAGAGGACCATCTAAAGCTTCCATCTGGTTGCCCTGGGACTAGTTTTGGTAGGCGTCATAGTGTGAGCAAATGTAAGCTCCCTTTCTATTGA

Coding sequence (CDS)

ATGGCTCTTCATCTTCCACCAATCGTAGATTCCCGAATGCAGAGGATTCCTGTTATGTCACAGAGCCCCCTTTCCCTAGCTTTAAATGGCGGTTGGTCATTGCTTATGACGGTACGCGATATGCAGGTTAATGGTAATTCTAATCGGCTTAAGCTTTTGAGACATGATTTGGGTTCCGGTGTGAATTATGAGTTAACTCAGATATTGCATTCCTGCGCTTACTACTACAATTTTGAGGCTCTTTTCTGCCTGATTGAAAATGCCTTGACTCGAGCAACAAAGCTTGAGAGGAAGGATCTCCGTTTAGTTGGTGCAAGCAGAACAGATACGGGAGTTCATGCCTGGGGTCAGGTGGCACACTTTATTACGCCATTTAACTATGATAGCTTGGATAACGTTCATGCTGCTTTGAATGGTCTTCTTCCTGCGGATATAAGAGTTCGGGAGATTAGTCCTGCTGTGCCTGACTTTCATGCTCGGTTTTCTACAATAAGCAAGGTTTATCACTACAAGATATATAATGGCACCATCTTGGATCCATTTCAGCGGTGCTACACTTACCATTGCGTTTATAAACTCAATTATGCAGCCATGAGAGAAGCTGCAAATTATTTCATTGGAAAGCATGATTTTTCTGCTTTTGCAAATGCAGCACATAATGATCGAGTGGTAGATCCAGTGAAGGTCATTTTTCGTTTTGACGTCATAGAAATGGGACCTCTTGTTCAGCTAGAAGTTGAGGGTTCTGGGTTCTTGTATAGACAAGTTCGGAATATGGTTGCCTTGTTGATTCAAATCGGAAAGGAAGCAATTGCTCCTGATGTTGTTCCTAAGATATTGGCCTCTGGAGATCGCAAGGAGCTTGCCAAGTATGCTCCGTGCATTCCTCCGCAGGGGCTCTGTCTTATGAGTATTAATTATAAAGAGGACCATCTAAAGCTTCCATCTGGTTGCCCTGGGACTAGTTTTGGTAGGCGTCATAGTGTGAGCAAATGTAAGCTCCCTTTCTATTGA

Protein sequence

MALHLPPIVDSRMQRIPVMSQSPLSLALNGGWSLLMTVRDMQVNGNSNRLKLLRHDLGSGVNYELTQILHSCAYYYNFEALFCLIENALTRATKLERKDLRLVGASRTDTGVHAWGQVAHFITPFNYDSLDNVHAALNGLLPADIRVREISPAVPDFHARFSTISKVYHYKIYNGTILDPFQRCYTYHCVYKLNYAAMREAANYFIGKHDFSAFANAAHNDRVVDPVKVIFRFDVIEMGPLVQLEVEGSGFLYRQVRNMVALLIQIGKEAIAPDVVPKILASGDRKELAKYAPCIPPQGLCLMSINYKEDHLKLPSGCPGTSFGRRHSVSKCKLPFY

Homology

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BLAST of Spg024955 vs. NCBI nr
Match: XP_008458280.1 (PREDICTED: tRNA pseudouridine synthase A 1 isoform X2 [Cucumis melo])

HSP 1 Score: 506.9 bits (1304), Expect = 1.3e-139
Identity = 253/337 (75.07%), Postives = 275/337 (81.60%), Query Frame = 0


Query: 1   MALHLPPIVDSRMQRIPVMSQSPLSLALNGGWSLLMTVRDMQVNGNSNRLKLLRHDLGSG 60
           MALHLPP+VDSRM+RIP++SQSPLS A NGG S LMTV DMQ +                
Sbjct: 1   MALHLPPVVDSRMRRIPLISQSPLSPASNGGCSSLMTVLDMQRSP--------------- 60

Query: 61  VNYELTQILHSCAYYYNFEALFCLIENALTRATKLERKDLRLVGASRTDTGVHAWGQVAH 120
                               + CLIE AL  ATKLERKDL LVGASRTD GVHAWGQVAH
Sbjct: 61  ------------------PTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAH 120

Query: 121 FITPFNYDSLDNVHAALNGLLPADIRVREISPAVPDFHARFSTISKVYHYKIYNGTILDP 180
           F+TPFNYDSL++VHAALNGLLP DIRVRE+SPA PDFHARFST+SKVYHYKIYNGTI+DP
Sbjct: 121 FVTPFNYDSLESVHAALNGLLPVDIRVREMSPAAPDFHARFSTLSKVYHYKIYNGTIMDP 180

Query: 181 FQRCYTYHCVYKLNYAAMREAANYFIGKHDFSAFANAAHNDRVVDPVKVIFRFDVIEMGP 240
           FQRCYTYHCVYKLNYAAM+EAANYF+GKHDFSAF NAAHN+RVV+PVKVIFRFDVIEMG 
Sbjct: 181 FQRCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNNRVVNPVKVIFRFDVIEMGA 240

Query: 241 LVQLEVEGSGFLYRQVRNMVALLIQIGKEAIAPDVVPKILASGDRKELAKYAPCIPPQGL 300
           LVQLEVEGSGFLYRQVRNMV LL+QIGKEA+ P+VVPKILAS DRKELAKYA  +PPQGL
Sbjct: 241 LVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRKELAKYASGVPPQGL 300

Query: 301 CLMSINYKEDHLKLPSGCPGTSFGRRHSVSKCKLPFY 338
           CL+SINYKEDHLKLPS CPGTS GR  SV KCK+PFY
Sbjct: 301 CLVSINYKEDHLKLPSDCPGTSLGRSRSVGKCKVPFY 304

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BLAST of Spg024955 vs. NCBI nr
Match: XP_023549334.1 (uncharacterized protein LOC111807720 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 483.0 bits (1242), Expect = 2.1e-132
Identity = 229/255 (89.80%), Postives = 240/255 (94.12%), Query Frame = 0


Query: 83  CLIENALTRATKLERKDLRLVGASRTDTGVHAWGQVAHFITPFNYDSLDNVHAALNGLLP 142
           CLIE ALT AT+LERKDL LVGASRTDTGVHAWGQVAHFITPFNYDSLD +HAALNGLLP
Sbjct: 81  CLIEEALTGATRLERKDLHLVGASRTDTGVHAWGQVAHFITPFNYDSLDRIHAALNGLLP 140

Query: 143 ADIRVREISPAVPDFHARFSTISKVYHYKIYNGTILDPFQRCYTYHCVYKLNYAAMREAA 202
           ADIRVREISPAVPDFHARFST+SKVYHYKIYN  ILDPFQRCYTYHCVYKLNYAAM+EAA
Sbjct: 141 ADIRVREISPAVPDFHARFSTLSKVYHYKIYNDAILDPFQRCYTYHCVYKLNYAAMKEAA 200

Query: 203 NYFIGKHDFSAFANAAHNDRVVDPVKVIFRFDVIEMGPLVQLEVEGSGFLYRQVRNMVAL 262
           NYF+GKHDFSAFANAAHNDRVV+PVKV+FRFDVIEMG L+QLEVEGSGFLYRQVRNMVAL
Sbjct: 201 NYFVGKHDFSAFANAAHNDRVVNPVKVVFRFDVIEMGALIQLEVEGSGFLYRQVRNMVAL 260

Query: 263 LIQIGKEAIAPDVVPKILASGDRKELAKYAPCIPPQGLCLMSINYKEDHLKLPSGCPGTS 322
           L+QIGKEAI PDVVPKILA+ DRKELAKY  CIPP GLCL+ INYKEDHLKLPS CPGTS
Sbjct: 261 LLQIGKEAIPPDVVPKILATRDRKELAKYTTCIPPHGLCLVRINYKEDHLKLPSDCPGTS 320

Query: 323 FGRRHSVSKCKLPFY 338
           FGR HSVSKCKLPFY
Sbjct: 321 FGRSHSVSKCKLPFY 335

[Close]

BLAST of Spg024955 vs. NCBI nr
Match: XP_023549333.1 (uncharacterized protein LOC111807720 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 483.0 bits (1242), Expect = 2.1e-132
Identity = 229/255 (89.80%), Postives = 240/255 (94.12%), Query Frame = 0


Query: 83  CLIENALTRATKLERKDLRLVGASRTDTGVHAWGQVAHFITPFNYDSLDNVHAALNGLLP 142
           CLIE ALT AT+LERKDL LVGASRTDTGVHAWGQVAHFITPFNYDSLD +HAALNGLLP
Sbjct: 83  CLIEEALTGATRLERKDLHLVGASRTDTGVHAWGQVAHFITPFNYDSLDRIHAALNGLLP 142

Query: 143 ADIRVREISPAVPDFHARFSTISKVYHYKIYNGTILDPFQRCYTYHCVYKLNYAAMREAA 202
           ADIRVREISPAVPDFHARFST+SKVYHYKIYN  ILDPFQRCYTYHCVYKLNYAAM+EAA
Sbjct: 143 ADIRVREISPAVPDFHARFSTLSKVYHYKIYNDAILDPFQRCYTYHCVYKLNYAAMKEAA 202

Query: 203 NYFIGKHDFSAFANAAHNDRVVDPVKVIFRFDVIEMGPLVQLEVEGSGFLYRQVRNMVAL 262
           NYF+GKHDFSAFANAAHNDRVV+PVKV+FRFDVIEMG L+QLEVEGSGFLYRQVRNMVAL
Sbjct: 203 NYFVGKHDFSAFANAAHNDRVVNPVKVVFRFDVIEMGALIQLEVEGSGFLYRQVRNMVAL 262

Query: 263 LIQIGKEAIAPDVVPKILASGDRKELAKYAPCIPPQGLCLMSINYKEDHLKLPSGCPGTS 322
           L+QIGKEAI PDVVPKILA+ DRKELAKY  CIPP GLCL+ INYKEDHLKLPS CPGTS
Sbjct: 263 LLQIGKEAIPPDVVPKILATRDRKELAKYTTCIPPHGLCLVRINYKEDHLKLPSDCPGTS 322

Query: 323 FGRRHSVSKCKLPFY 338
           FGR HSVSKCKLPFY
Sbjct: 323 FGRSHSVSKCKLPFY 337

[Close]

BLAST of Spg024955 vs. NCBI nr
Match: XP_023549335.1 (uncharacterized protein LOC111807720 isoform X3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 483.0 bits (1242), Expect = 2.1e-132
Identity = 229/255 (89.80%), Postives = 240/255 (94.12%), Query Frame = 0


Query: 83  CLIENALTRATKLERKDLRLVGASRTDTGVHAWGQVAHFITPFNYDSLDNVHAALNGLLP 142
           CLIE ALT AT+LERKDL LVGASRTDTGVHAWGQVAHFITPFNYDSLD +HAALNGLLP
Sbjct: 81  CLIEEALTGATRLERKDLHLVGASRTDTGVHAWGQVAHFITPFNYDSLDRIHAALNGLLP 140

Query: 143 ADIRVREISPAVPDFHARFSTISKVYHYKIYNGTILDPFQRCYTYHCVYKLNYAAMREAA 202
           ADIRVREISPAVPDFHARFST+SKVYHYKIYN  ILDPFQRCYTYHCVYKLNYAAM+EAA
Sbjct: 141 ADIRVREISPAVPDFHARFSTLSKVYHYKIYNDAILDPFQRCYTYHCVYKLNYAAMKEAA 200

Query: 203 NYFIGKHDFSAFANAAHNDRVVDPVKVIFRFDVIEMGPLVQLEVEGSGFLYRQVRNMVAL 262
           NYF+GKHDFSAFANAAHNDRVV+PVKV+FRFDVIEMG L+QLEVEGSGFLYRQVRNMVAL
Sbjct: 201 NYFVGKHDFSAFANAAHNDRVVNPVKVVFRFDVIEMGALIQLEVEGSGFLYRQVRNMVAL 260

Query: 263 LIQIGKEAIAPDVVPKILASGDRKELAKYAPCIPPQGLCLMSINYKEDHLKLPSGCPGTS 322
           L+QIGKEAI PDVVPKILA+ DRKELAKY  CIPP GLCL+ INYKEDHLKLPS CPGTS
Sbjct: 261 LLQIGKEAIPPDVVPKILATRDRKELAKYTTCIPPHGLCLVRINYKEDHLKLPSDCPGTS 320

Query: 323 FGRRHSVSKCKLPFY 338
           FGR HSVSKCKLPFY
Sbjct: 321 FGRSHSVSKCKLPFY 335

[Close]

BLAST of Spg024955 vs. NCBI nr
Match: XP_023549337.1 (uncharacterized protein LOC111807720 isoform X4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 483.0 bits (1242), Expect = 2.1e-132
Identity = 229/255 (89.80%), Postives = 240/255 (94.12%), Query Frame = 0


Query: 83  CLIENALTRATKLERKDLRLVGASRTDTGVHAWGQVAHFITPFNYDSLDNVHAALNGLLP 142
           CLIE ALT AT+LERKDL LVGASRTDTGVHAWGQVAHFITPFNYDSLD +HAALNGLLP
Sbjct: 79  CLIEEALTGATRLERKDLHLVGASRTDTGVHAWGQVAHFITPFNYDSLDRIHAALNGLLP 138

Query: 143 ADIRVREISPAVPDFHARFSTISKVYHYKIYNGTILDPFQRCYTYHCVYKLNYAAMREAA 202
           ADIRVREISPAVPDFHARFST+SKVYHYKIYN  ILDPFQRCYTYHCVYKLNYAAM+EAA
Sbjct: 139 ADIRVREISPAVPDFHARFSTLSKVYHYKIYNDAILDPFQRCYTYHCVYKLNYAAMKEAA 198

Query: 203 NYFIGKHDFSAFANAAHNDRVVDPVKVIFRFDVIEMGPLVQLEVEGSGFLYRQVRNMVAL 262
           NYF+GKHDFSAFANAAHNDRVV+PVKV+FRFDVIEMG L+QLEVEGSGFLYRQVRNMVAL
Sbjct: 199 NYFVGKHDFSAFANAAHNDRVVNPVKVVFRFDVIEMGALIQLEVEGSGFLYRQVRNMVAL 258

Query: 263 LIQIGKEAIAPDVVPKILASGDRKELAKYAPCIPPQGLCLMSINYKEDHLKLPSGCPGTS 322
           L+QIGKEAI PDVVPKILA+ DRKELAKY  CIPP GLCL+ INYKEDHLKLPS CPGTS
Sbjct: 259 LLQIGKEAIPPDVVPKILATRDRKELAKYTTCIPPHGLCLVRINYKEDHLKLPSDCPGTS 318

Query: 323 FGRRHSVSKCKLPFY 338
           FGR HSVSKCKLPFY
Sbjct: 319 FGRSHSVSKCKLPFY 333

[Close]

BLAST of Spg024955 vs. ExPASy Swiss-Prot
Match: Q6MEE7 (tRNA pseudouridine synthase A 1 OS=Protochlamydia amoebophila (strain UWE25) OX=264201 GN=truA1 PE=3 SV=1)

HSP 1 Score: 174.9 bits (442), Expect = 1.6e-42
Identity = 87/218 (39.91%), Postives = 139/218 (63.76%), Query Frame = 0


Query: 95  LERKDLRLVGASRTDTGVHAWGQVAHFITPFNYDSLDNVHAALNGLLPADIRVREISPAV 154
           L+++ + LVG+ RTD GVHA GQVAHF    +Y  L  +H +LNGLLP DIR++ + P  
Sbjct: 39  LKKEKVVLVGSGRTDAGVHAKGQVAHFHFQ-DYIDLSRLHVSLNGLLPRDIRIKAVEPVS 98

Query: 155 PDFHARFSTISKVYHYKIYNGTILDPFQRCYTYHCVYKLNYAAMREAANYFIGKHDFSAF 214
           P FH+++S I K YHY ++   ++DPFQR Y++H   K++   +++AA  F G HDF++F
Sbjct: 99  PRFHSQYSAIRKEYHYYLHLNKVMDPFQRLYSWHFQRKIDVNILKKAAILFTGTHDFTSF 158

Query: 215 ANAAHNDRVV-DPVKVIFRFDVIEMGPLVQLEVEGSGFLYRQVRNMVALLIQIGKEAIAP 274
           AN AH      +PV+ ++R D+      ++LE EG GFLY+ VRN+V  L+ +     A 
Sbjct: 159 ANEAHRGTAAKNPVRTLYRLDIKPNEGGLRLEFEGDGFLYKMVRNIVGTLMDVASHKRAI 218

Query: 275 DVVPKILASGDRKELAKYAPCIPPQGLCLMSINYKEDH 312
           + + +I A+ +R++ +  A   PP+GL L+ + Y+ ++
Sbjct: 219 EEINQIFAAKNRRQASLAA---PPEGLFLIQVFYENEN 252

[Close]

BLAST of Spg024955 vs. ExPASy Swiss-Prot
Match: B1I1B5 (tRNA pseudouridine synthase A OS=Desulforudis audaxviator (strain MP104C) OX=477974 GN=truA PE=3 SV=1)

HSP 1 Score: 174.5 bits (441), Expect = 2.1e-42
Identity = 94/224 (41.96%), Postives = 128/224 (57.14%), Query Frame = 0


Query: 85  IENALTRA-TKLERKDLRLVGASRTDTGVHAWGQVAHFITPFNYDSLDNVHAALNGLLPA 144
           I+  L R   +L    L++ GASRTD GVHA GQV +F+T       + + AALNG+LP 
Sbjct: 29  IQETLERCLAELSGAALKVTGASRTDAGVHARGQVVNFVTGRWGIPTERIPAALNGVLPG 88

Query: 145 DIRVREISPAVPDFHARFSTISKVYHYKIYNGTILDPFQRCYTYHCVYKLNYAAMREAAN 204
           DI   +      DFHAR+S ++K Y Y +YN  +  PFQR Y+ H    L+  AMR AA 
Sbjct: 89  DIAALDAREVPADFHARYSAVAKTYSYTLYNHPVRSPFQRLYSLHVPRALDLRAMRLAAR 148

Query: 205 YFIGKHDFSAFANAAHNDRVVDPVKVIFRFDVIEMGPLVQLEVEGSGFLYRQVRNMVALL 264
           + +G+HDFS F  A     V   V+ + R D+    PLV+L     GFLY  VR M   L
Sbjct: 149 HLMGRHDFSTFQAAGR--PVQSAVRTLTRADIEAQAPLVRLVFRADGFLYNMVRIMAGTL 208

Query: 265 IQIGKEAIAPDVVPKILASGDRKELAKYAPCIPPQGLCLMSINY 308
           +++G E + P+ +P I+ASGDR   A+  P +PP GLCL  + Y
Sbjct: 209 LEVGLERLDPEALPDIIASGDR---ARAGPTVPPHGLCLEEVEY 247

[Close]

BLAST of Spg024955 vs. ExPASy Swiss-Prot
Match: A4J151 (tRNA pseudouridine synthase A OS=Desulfotomaculum reducens (strain MI-1) OX=349161 GN=truA PE=3 SV=1)

HSP 1 Score: 162.9 bits (411), Expect = 6.2e-39
Identity = 90/224 (40.18%), Postives = 130/224 (58.04%), Query Frame = 0


Query: 85  IENALTRA-TKLERKDLRLVGASRTDTGVHAWGQVAHFITPFNYDSLDNVHAALNGLLPA 144
           I+ AL +A + + +  ++++GA RTD GVHA GQV +F +       +    ALN LLP 
Sbjct: 29  IQEALEKALSTIAQTPIQVIGAGRTDAGVHAQGQVVNFRSEKWPVPAEKAPLALNVLLPG 88

Query: 145 DIRVREISPAVPDFHARFSTISKVYHYKIYNGTILDPFQRCYTYHCVYKLNYAAMREAAN 204
           DI+V +      DFHARFS ++K Y Y IY+  ++ PF R Y YH   +L+  AM+E A 
Sbjct: 89  DIKVVKAEEVPMDFHARFSAVAKTYRYSIYHHRVMSPFHRYYCYHEPRRLDVNAMQEGAA 148

Query: 205 YFIGKHDFSAFANAAHNDRVVDPVKVIFRFDVIEMGPLVQLEVEGSGFLYRQVRNMVALL 264
           Y +G +DF +F   A    V D ++ I+R D+IE  P++ L + G+GFLY  VR +   L
Sbjct: 149 YLLGTYDFKSF--QAQGTPVKDTIRTIYRADIIEDAPVINLYLRGNGFLYNMVRIITGTL 208

Query: 265 IQIGKEAIAPDVVPKILASGDRKELAKYAPCIPPQGLCLMSINY 308
           + IG   I P+ + KI+ S +R      A   PPQGLCLM + Y
Sbjct: 209 LNIGFGKIKPEDMVKIIESKNRTLAGTTA---PPQGLCLMEVEY 247

[Close]

BLAST of Spg024955 vs. ExPASy Swiss-Prot
Match: P70973 (tRNA pseudouridine synthase A OS=Bacillus subtilis (strain 168) OX=224308 GN=truA PE=3 SV=2)

HSP 1 Score: 149.1 bits (375), Expect = 9.2e-35
Identity = 90/223 (40.36%), Postives = 122/223 (54.71%), Query Frame = 0


Query: 85  IENALTRATKLERKDLRLVGASRTDTGVHAWGQVAHFITPFNYDSLDNVHAALNGLLPAD 144
           +E AL    K  +  + +V + RTD+GVHA GQV HF TP +  + +    ALN LLP D
Sbjct: 30  LEKALAVLHK-SKDRIPVVSSGRTDSGVHAAGQVIHFDTPLSIPA-ERWPYALNALLPDD 89

Query: 145 IRVREISPAVPDFHARFSTISKVYHYKIYNGTILDPFQRCYTYHCVYKLNYAAMREAANY 204
           I V++   A   FHARFS + K Y Y +Y     D F+R Y YH  Y+LN   MREAA +
Sbjct: 90  IAVKQAEIADDGFHARFSAVKKEYRYFVYTEKHPDVFKRHYAYHFSYRLNVQDMREAAKH 149

Query: 205 FIGKHDFSAFANAAHNDRVVDPVKVIFRFDVIEMGPLVQLEVEGSGFLYRQVRNMVALLI 264
            IG HDF++F   A    V D V+ I+  D  E    +Q+ + GSGFLY  VR +   L+
Sbjct: 150 LIGTHDFTSF--CAAKTEVQDKVRTIYELDWTETADGLQMRITGSGFLYNMVRIIAGTLL 209

Query: 265 QIGKEAIAPDVVPKILASGDRKELAKYAPCIPPQGLCLMSINY 308
             G   I+PD V  +L + DR+   + A   P  GL L ++ Y
Sbjct: 210 DAGIGKISPDEVKSMLEAKDREAAGRTA---PGHGLYLWNVYY 245

[Close]

BLAST of Spg024955 vs. ExPASy Swiss-Prot
Match: A3DJK8 (tRNA pseudouridine synthase A OS=Hungateiclostridium thermocellum (strain ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) OX=203119 GN=truA PE=3 SV=1)

HSP 1 Score: 148.7 bits (374), Expect = 1.2e-34
Identity = 87/224 (38.84%), Postives = 126/224 (56.25%), Query Frame = 0


Query: 85  IENALTRATK-LERKDLRLVGASRTDTGVHAWGQVAHFITPFNYDSLDNVHAALNGLLPA 144
           +++ + RA K L  + + L+GASRTD GVHA GQVA+FIT       D    ALN +LP 
Sbjct: 27  VQDTIERAIKGLTGEKVDLIGASRTDFGVHALGQVANFITNSGIPG-DRFSYALNRMLPD 86

Query: 145 DIRVREISPAVPDFHARFSTISKVYHYKIYNGTILDPFQRCYTYHCVYKLNYAAMREAAN 204
           DI ++E      DFHAR+    K Y Y IYN        R  TYH  +KL++ +M+ AA+
Sbjct: 87  DIVIKESQEVDMDFHARYKAKGKRYRYLIYNSQFPSALLRHRTYHVSHKLDFESMQRAAS 146

Query: 205 YFIGKHDFSAFANAAHNDRVVDPVKVIFRFDVIEMGPLVQLEVEGSGFLYRQVRNMVALL 264
           YF+G HDFSAF ++  + +    V+ I    +     +V+ E+ G GFLY  VR +V  L
Sbjct: 147 YFLGTHDFSAFRSSGSSAKT--SVRTITDVSLEREDKIVKFEIAGDGFLYNMVRIIVGTL 206

Query: 265 IQIGKEAIAPDVVPKILASGDRKELAKYAPCIPPQGLCLMSINY 308
           +++G   I  D +P+I+ S  RK   + AP    +GL L+ + Y
Sbjct: 207 MEVGIGKIRADEIPQIIESRKRKRAGRTAPA---EGLYLVEVYY 244

[Close]

BLAST of Spg024955 vs. ExPASy TrEMBL
Match: A0A1S3C7H4 (tRNA pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103497746 PE=3 SV=1)

HSP 1 Score: 506.9 bits (1304), Expect = 6.5e-140
Identity = 253/337 (75.07%), Postives = 275/337 (81.60%), Query Frame = 0


Query: 1   MALHLPPIVDSRMQRIPVMSQSPLSLALNGGWSLLMTVRDMQVNGNSNRLKLLRHDLGSG 60
           MALHLPP+VDSRM+RIP++SQSPLS A NGG S LMTV DMQ +                
Sbjct: 1   MALHLPPVVDSRMRRIPLISQSPLSPASNGGCSSLMTVLDMQRSP--------------- 60

Query: 61  VNYELTQILHSCAYYYNFEALFCLIENALTRATKLERKDLRLVGASRTDTGVHAWGQVAH 120
                               + CLIE AL  ATKLERKDL LVGASRTD GVHAWGQVAH
Sbjct: 61  ------------------PTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAH 120

Query: 121 FITPFNYDSLDNVHAALNGLLPADIRVREISPAVPDFHARFSTISKVYHYKIYNGTILDP 180
           F+TPFNYDSL++VHAALNGLLP DIRVRE+SPA PDFHARFST+SKVYHYKIYNGTI+DP
Sbjct: 121 FVTPFNYDSLESVHAALNGLLPVDIRVREMSPAAPDFHARFSTLSKVYHYKIYNGTIMDP 180

Query: 181 FQRCYTYHCVYKLNYAAMREAANYFIGKHDFSAFANAAHNDRVVDPVKVIFRFDVIEMGP 240
           FQRCYTYHCVYKLNYAAM+EAANYF+GKHDFSAF NAAHN+RVV+PVKVIFRFDVIEMG 
Sbjct: 181 FQRCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNNRVVNPVKVIFRFDVIEMGA 240

Query: 241 LVQLEVEGSGFLYRQVRNMVALLIQIGKEAIAPDVVPKILASGDRKELAKYAPCIPPQGL 300
           LVQLEVEGSGFLYRQVRNMV LL+QIGKEA+ P+VVPKILAS DRKELAKYA  +PPQGL
Sbjct: 241 LVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRKELAKYASGVPPQGL 300

Query: 301 CLMSINYKEDHLKLPSGCPGTSFGRRHSVSKCKLPFY 338
           CL+SINYKEDHLKLPS CPGTS GR  SV KCK+PFY
Sbjct: 301 CLVSINYKEDHLKLPSDCPGTSLGRSRSVGKCKVPFY 304

[Close]

BLAST of Spg024955 vs. ExPASy TrEMBL
Match: A0A6J1H621 (tRNA pseudouridine synthase OS=Cucurbita moschata OX=3662 GN=LOC111460439 PE=3 SV=1)

HSP 1 Score: 481.9 bits (1239), Expect = 2.2e-132
Identity = 227/255 (89.02%), Postives = 240/255 (94.12%), Query Frame = 0


Query: 83  CLIENALTRATKLERKDLRLVGASRTDTGVHAWGQVAHFITPFNYDSLDNVHAALNGLLP 142
           CL+E ALT AT+LERKDL LVGASRTDTGVHAWGQVAHFITPFNYDSLD +HAALNGLLP
Sbjct: 80  CLVEEALTGATRLERKDLHLVGASRTDTGVHAWGQVAHFITPFNYDSLDRIHAALNGLLP 139

Query: 143 ADIRVREISPAVPDFHARFSTISKVYHYKIYNGTILDPFQRCYTYHCVYKLNYAAMREAA 202
           ADIRVRE+SPAVPDFHARFST+SKVYHYKIYN  ILDPFQRCYTYHCVYKLNYAAM+EAA
Sbjct: 140 ADIRVREMSPAVPDFHARFSTLSKVYHYKIYNDAILDPFQRCYTYHCVYKLNYAAMKEAA 199

Query: 203 NYFIGKHDFSAFANAAHNDRVVDPVKVIFRFDVIEMGPLVQLEVEGSGFLYRQVRNMVAL 262
           NYF+GKHDFSAFAN AHNDRVV+PVKVIFRFDVIEMG L+QLEVEGSGFLYRQVRNMVAL
Sbjct: 200 NYFVGKHDFSAFANTAHNDRVVNPVKVIFRFDVIEMGALIQLEVEGSGFLYRQVRNMVAL 259

Query: 263 LIQIGKEAIAPDVVPKILASGDRKELAKYAPCIPPQGLCLMSINYKEDHLKLPSGCPGTS 322
           L+QIGKEAI PD+VPKILA+ DRKELAKY  CIPPQGLCL+ INYKEDHLKLPS CPGTS
Sbjct: 260 LLQIGKEAIPPDIVPKILATRDRKELAKYTTCIPPQGLCLVRINYKEDHLKLPSDCPGTS 319

Query: 323 FGRRHSVSKCKLPFY 338
           FGR HSVSKCKLPFY
Sbjct: 320 FGRSHSVSKCKLPFY 334

[Close]

BLAST of Spg024955 vs. ExPASy TrEMBL
Match: A0A6J1H4M7 (tRNA pseudouridine synthase OS=Cucurbita moschata OX=3662 GN=LOC111460439 PE=3 SV=1)

HSP 1 Score: 481.9 bits (1239), Expect = 2.2e-132
Identity = 227/255 (89.02%), Postives = 240/255 (94.12%), Query Frame = 0


Query: 83  CLIENALTRATKLERKDLRLVGASRTDTGVHAWGQVAHFITPFNYDSLDNVHAALNGLLP 142
           CL+E ALT AT+LERKDL LVGASRTDTGVHAWGQVAHFITPFNYDSLD +HAALNGLLP
Sbjct: 82  CLVEEALTGATRLERKDLHLVGASRTDTGVHAWGQVAHFITPFNYDSLDRIHAALNGLLP 141

Query: 143 ADIRVREISPAVPDFHARFSTISKVYHYKIYNGTILDPFQRCYTYHCVYKLNYAAMREAA 202
           ADIRVRE+SPAVPDFHARFST+SKVYHYKIYN  ILDPFQRCYTYHCVYKLNYAAM+EAA
Sbjct: 142 ADIRVREMSPAVPDFHARFSTLSKVYHYKIYNDAILDPFQRCYTYHCVYKLNYAAMKEAA 201

Query: 203 NYFIGKHDFSAFANAAHNDRVVDPVKVIFRFDVIEMGPLVQLEVEGSGFLYRQVRNMVAL 262
           NYF+GKHDFSAFAN AHNDRVV+PVKVIFRFDVIEMG L+QLEVEGSGFLYRQVRNMVAL
Sbjct: 202 NYFVGKHDFSAFANTAHNDRVVNPVKVIFRFDVIEMGALIQLEVEGSGFLYRQVRNMVAL 261

Query: 263 LIQIGKEAIAPDVVPKILASGDRKELAKYAPCIPPQGLCLMSINYKEDHLKLPSGCPGTS 322
           L+QIGKEAI PD+VPKILA+ DRKELAKY  CIPPQGLCL+ INYKEDHLKLPS CPGTS
Sbjct: 262 LLQIGKEAIPPDIVPKILATRDRKELAKYTTCIPPQGLCLVRINYKEDHLKLPSDCPGTS 321

Query: 323 FGRRHSVSKCKLPFY 338
           FGR HSVSKCKLPFY
Sbjct: 322 FGRSHSVSKCKLPFY 336

[Close]

BLAST of Spg024955 vs. ExPASy TrEMBL
Match: A0A6J1L0L1 (tRNA pseudouridine synthase OS=Cucurbita maxima OX=3661 GN=LOC111499282 PE=3 SV=1)

HSP 1 Score: 479.9 bits (1234), Expect = 8.4e-132
Identity = 229/255 (89.80%), Postives = 238/255 (93.33%), Query Frame = 0


Query: 83  CLIENALTRATKLERKDLRLVGASRTDTGVHAWGQVAHFITPFNYDSLDNVHAALNGLLP 142
           CLIE ALT AT+LERKDL LVGASRTD GVHAWGQVAHFITPFNYDSLD +HAALNGLLP
Sbjct: 79  CLIEEALTGATRLERKDLHLVGASRTDKGVHAWGQVAHFITPFNYDSLDRIHAALNGLLP 138

Query: 143 ADIRVREISPAVPDFHARFSTISKVYHYKIYNGTILDPFQRCYTYHCVYKLNYAAMREAA 202
           ADIRVREISPAVPDFHARFST+SKVYHYKIYN  ILDPFQRCYTYHCVYKLNYAAM+EAA
Sbjct: 139 ADIRVREISPAVPDFHARFSTLSKVYHYKIYNDAILDPFQRCYTYHCVYKLNYAAMKEAA 198

Query: 203 NYFIGKHDFSAFANAAHNDRVVDPVKVIFRFDVIEMGPLVQLEVEGSGFLYRQVRNMVAL 262
           NYF+GKHDFSAFAN AHNDRVV+PVKVIFRFDVIEMG L+QLEVEGSGFLYRQVRNMVAL
Sbjct: 199 NYFVGKHDFSAFANTAHNDRVVNPVKVIFRFDVIEMGALIQLEVEGSGFLYRQVRNMVAL 258

Query: 263 LIQIGKEAIAPDVVPKILASGDRKELAKYAPCIPPQGLCLMSINYKEDHLKLPSGCPGTS 322
           L+QIGKEAI PDVVPKILAS DRK LAKY  CIPPQGLCL+ INYKEDHLKLPS CPGTS
Sbjct: 259 LLQIGKEAIPPDVVPKILASRDRKVLAKYTTCIPPQGLCLVRINYKEDHLKLPSDCPGTS 318

Query: 323 FGRRHSVSKCKLPFY 338
           FGR HSVSKCKLPFY
Sbjct: 319 FGRSHSVSKCKLPFY 333

[Close]

BLAST of Spg024955 vs. ExPASy TrEMBL
Match: A0A6J1L2M8 (tRNA pseudouridine synthase OS=Cucurbita maxima OX=3661 GN=LOC111499282 PE=3 SV=1)

HSP 1 Score: 479.9 bits (1234), Expect = 8.4e-132
Identity = 229/255 (89.80%), Postives = 238/255 (93.33%), Query Frame = 0


Query: 83  CLIENALTRATKLERKDLRLVGASRTDTGVHAWGQVAHFITPFNYDSLDNVHAALNGLLP 142
           CLIE ALT AT+LERKDL LVGASRTD GVHAWGQVAHFITPFNYDSLD +HAALNGLLP
Sbjct: 81  CLIEEALTGATRLERKDLHLVGASRTDKGVHAWGQVAHFITPFNYDSLDRIHAALNGLLP 140

Query: 143 ADIRVREISPAVPDFHARFSTISKVYHYKIYNGTILDPFQRCYTYHCVYKLNYAAMREAA 202
           ADIRVREISPAVPDFHARFST+SKVYHYKIYN  ILDPFQRCYTYHCVYKLNYAAM+EAA
Sbjct: 141 ADIRVREISPAVPDFHARFSTLSKVYHYKIYNDAILDPFQRCYTYHCVYKLNYAAMKEAA 200

Query: 203 NYFIGKHDFSAFANAAHNDRVVDPVKVIFRFDVIEMGPLVQLEVEGSGFLYRQVRNMVAL 262
           NYF+GKHDFSAFAN AHNDRVV+PVKVIFRFDVIEMG L+QLEVEGSGFLYRQVRNMVAL
Sbjct: 201 NYFVGKHDFSAFANTAHNDRVVNPVKVIFRFDVIEMGALIQLEVEGSGFLYRQVRNMVAL 260

Query: 263 LIQIGKEAIAPDVVPKILASGDRKELAKYAPCIPPQGLCLMSINYKEDHLKLPSGCPGTS 322
           L+QIGKEAI PDVVPKILAS DRK LAKY  CIPPQGLCL+ INYKEDHLKLPS CPGTS
Sbjct: 261 LLQIGKEAIPPDVVPKILASRDRKVLAKYTTCIPPQGLCLVRINYKEDHLKLPSDCPGTS 320

Query: 323 FGRRHSVSKCKLPFY 338
           FGR HSVSKCKLPFY
Sbjct: 321 FGRSHSVSKCKLPFY 335

[Close]

BLAST of Spg024955 vs. TAIR 10
Match: AT3G06950.1 (Pseudouridine synthase family protein )

HSP 1 Score: 373.6 bits (958), Expect = 1.6e-103
Identity = 179/255 (70.20%), Postives = 207/255 (81.18%), Query Frame = 0


Query: 84  LIENALTRATKLERKDLRLVGASRTDTGVHAWGQVAHFITPFNYDSLDNVHAALNGLLPA 143
           ++E AL + T+L RK+L+L+GA RTD GVHAWGQVAHF+TPFNY SLD+ HAALNGLLP 
Sbjct: 69  MLEKALIQITELGRKELQLIGAGRTDAGVHAWGQVAHFVTPFNYTSLDSFHAALNGLLPK 128

Query: 144 DIRVREISPAVPDFHARFSTISKVYHYKIYNGTILDPFQRCYTYHCVYKLNYAAMREAAN 203
           DIRVRE+S AVP+FHARFS  SKVY Y+IYN T +DPFQR + YHC YKLN + MREAAN
Sbjct: 129 DIRVRELSAAVPEFHARFSASSKVYRYQIYNDTFMDPFQRHWAYHCAYKLNASKMREAAN 188

Query: 204 YFIGKHDFSAFANAAHNDRVVDPVKVIFRFDVIEMGPLVQLEVEGSGFLYRQVRNMVALL 263
            F+GKHDFSAFANA   D V DP+K I RFDVI+MG L+QLEVEGSGFLYRQVRNMVALL
Sbjct: 189 LFVGKHDFSAFANATREDGVPDPLKTISRFDVIQMGSLLQLEVEGSGFLYRQVRNMVALL 248

Query: 264 IQIGKEAIAPDVVPKILASGDRKELAKYAPC-IPPQGLCLMSINYKEDHLKLPSGCPGTS 323
           IQIGKEA+  D+VP IL + DR+ LAKY      P GLCL+S+ YKEDHLKLP  CP TS
Sbjct: 249 IQIGKEALDSDIVPMILETKDRRVLAKYTSLPASPHGLCLVSVKYKEDHLKLPLDCPVTS 308

Query: 324 FGRRHSVSKCKLPFY 338
            GR H+++KCKLPFY
Sbjct: 309 SGRHHTITKCKLPFY 323

[Close]

BLAST of Spg024955 vs. TAIR 10
Match: AT1G34150.1 (Pseudouridine synthase family protein )

HSP 1 Score: 71.2 bits (173), Expect = 1.7e-12
Identity = 69/213 (32.39%), Postives = 88/213 (41.31%), Query Frame = 0


Query: 90  TRATKLERKDLRLVGASRTDTGVHAWGQVAHFITPFNY-----DSLDNVHA--------- 149
           TR    ++KD       RTD GV + GQV              DS   V+          
Sbjct: 127 TRLLVGDKKDSCYSRCGRTDKGVSSTGQVIALFLRSRLKSPPGDSKAQVNGRTGERPEYD 186

Query: 150 ---ALNGLLPADIRVREISPAVPDFHARFSTISKVYHYKIYNGTILDPFQRCYTYHCVYK 209
               LN  LP DIRV   SPA  DFHARFS  ++ Y Y  +                   
Sbjct: 187 YVRVLNRALPDDIRVIGWSPAPIDFHARFSCYAREYKYFFWR----------------QN 246

Query: 210 LNYAAMREAANYFIGKHDFSAF-----ANAAHNDRVVDPVKVIFRFDVIEMGPLVQLEVE 269
           LN +AM  A   FIG+HDF  F     AN     R V   +V    +  E   L    + 
Sbjct: 247 LNLSAMDFAGKKFIGEHDFRNFCKMDVANVHCYTRRVTFFEVSPCQNSHEGDQLCTFTMR 306

Query: 270 GSGFLYRQVRNMVALLIQIGKEAIAPDVVPKIL 281
           GS FL+ Q+R MVA+L  IG+   + DV+  +L
Sbjct: 307 GSAFLWHQIRCMVAVLFMIGQGVESVDVIDTLL 323

The following BLAST results are available for this feature:

BLAST of Spg024955 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008458280.1	1.3e-139	75.07	PREDICTED: tRNA pseudouridine synthase A 1 isoform X2 [Cucumis melo]	[more]
XP_023549334.1	2.1e-132	89.80	uncharacterized protein LOC111807720 isoform X2 [Cucurbita pepo subsp. pepo]	[more]
XP_023549333.1	2.1e-132	89.80	uncharacterized protein LOC111807720 isoform X1 [Cucurbita pepo subsp. pepo]	[more]
XP_023549335.1	2.1e-132	89.80	uncharacterized protein LOC111807720 isoform X3 [Cucurbita pepo subsp. pepo]	[more]
XP_023549337.1	2.1e-132	89.80	uncharacterized protein LOC111807720 isoform X4 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Spg024955 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6MEE7	1.6e-42	39.91	tRNA pseudouridine synthase A 1 OS=Protochlamydia amoebophila (strain UWE25) OX=...	[more]
B1I1B5	2.1e-42	41.96	tRNA pseudouridine synthase A OS=Desulforudis audaxviator (strain MP104C) OX=477...	[more]
A4J151	6.2e-39	40.18	tRNA pseudouridine synthase A OS=Desulfotomaculum reducens (strain MI-1) OX=3491...	[more]
P70973	9.2e-35	40.36	tRNA pseudouridine synthase A OS=Bacillus subtilis (strain 168) OX=224308 GN=tru...	[more]
A3DJK8	1.2e-34	38.84	tRNA pseudouridine synthase A OS=Hungateiclostridium thermocellum (strain ATCC 2...	[more]

BLAST of Spg024955 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3C7H4	6.5e-140	75.07	tRNA pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103497746 PE=3 SV=1	[more]
A0A6J1H621	2.2e-132	89.02	tRNA pseudouridine synthase OS=Cucurbita moschata OX=3662 GN=LOC111460439 PE=3 S...	[more]
A0A6J1H4M7	2.2e-132	89.02	tRNA pseudouridine synthase OS=Cucurbita moschata OX=3662 GN=LOC111460439 PE=3 S...	[more]
A0A6J1L0L1	8.4e-132	89.80	tRNA pseudouridine synthase OS=Cucurbita maxima OX=3661 GN=LOC111499282 PE=3 SV=...	[more]
A0A6J1L2M8	8.4e-132	89.80	tRNA pseudouridine synthase OS=Cucurbita maxima OX=3661 GN=LOC111499282 PE=3 SV=...	[more]

BLAST of Spg024955 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G06950.1	1.6e-103	70.20	Pseudouridine synthase family protein	[more]
AT1G34150.1	1.7e-12	32.39	Pseudouridine synthase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001406	Pseudouridine synthase I, TruA	TIGRFAM	TIGR00071	TIGR00071	coord: 81..293 e-value: 6.3E-45 score: 151.6
IPR001406	Pseudouridine synthase I, TruA	PANTHER	PTHR11142	PSEUDOURIDYLATE SYNTHASE	coord: 82..321
IPR001406	Pseudouridine synthase I, TruA	HAMAP	MF_00171	TruA	coord: 55..307 score: 26.922739
IPR001406	Pseudouridine synthase I, TruA	CDD	cd02570	PseudoU_synth_EcTruA	coord: 85..307 e-value: 5.04446E-92 score: 272.425
IPR020094	Pseudouridine synthase TruA/RsuA/RluB/E/F, N-terminal	GENE3D	3.30.70.580		coord: 73..163 e-value: 2.8E-17 score: 64.9
IPR020095	Pseudouridine synthase I, TruA, C-terminal	GENE3D	3.30.70.660		coord: 164..306 e-value: 3.3E-38 score: 132.6
IPR020097	Pseudouridine synthase I, TruA, alpha/beta domain	PFAM	PF01416	PseudoU_synth_1	coord: 201..307 e-value: 4.7E-24 score: 85.0
None	No IPR available	PIRSR	PIRSR001430-2	PIRSR001430-2	coord: 97..302 e-value: 6.3E-62 score: 206.8
None	No IPR available	PANTHER	PTHR11142:SF0	TRNA PSEUDOURIDINE SYNTHASE-LIKE 1	coord: 82..321
IPR020103	Pseudouridine synthase, catalytic domain superfamily	SUPERFAMILY	55120	Pseudouridine synthase	coord: 85..312

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg024955.1	Spg024955.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0034196	acylglycerol transport
biological_process	GO:1990052	ER to chloroplast lipid transport
biological_process	GO:0001522	pseudouridine synthesis
biological_process	GO:0008033	tRNA processing
biological_process	GO:0009451	RNA modification
molecular_function	GO:0070300	phosphatidic acid binding
molecular_function	GO:0003723	RNA binding
molecular_function	GO:0106029	tRNA pseudouridine synthase activity
molecular_function	GO:0009982	pseudouridine synthase activity