Spg023298 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg023298
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptionubiquitin-conjugating enzyme E2 E2-like
Locationscaffold13: 5516233 .. 5521547 (+)
RNA-Seq ExpressionSpg023298
SyntenySpg023298
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTCAAAGTCAAGTAAACGCTTACTTCTAATTTTTTAGCTCTCACTTTCCCGTTCCGATCCAATCTCTCTATCTCCGCCGCCATGGTTCTTTCCCGTCTCCGCCGTTGCTTCTCTGCAAAATCCTCCTCTTCCACCGCCTACCTCCGCCTCCACACTCCGCCGCGCCCGGTTCCGATCGTCGACGAAGATTTCAAACGACCAGAGCCTGCGCCGCCGCGGCCGGTTCCGATCGTCGTCGTCGAGGATTTTAAACGGGCGGAGGATGCGCCGCCGCAGCCGATTCCGACCGTCGAGGGAGATGGTGACCGGAGAGAGGCCGCGTCGACGCGTCGTGTTACGATTAGCGAGGGCGGAAGCGGAAACGAAGAGAAAAGCAGTGCGATGTATCGGCTTGAGAACGAATTGAGAGCGATGGAGGAGGAGGCGCCGACGCACTGTAGCTTCGGGCCGGTCGGAGAGGACAGTTTCCGGTGGGAAGCGGCTGTAATTGGGCCGGCGTATTCTTGCTACGAAGGCGGTGTTTTTCACCTTTCCATTCAATTTCCCTCCGACTACCCCTTGAGGCCTCCCTGCATCAAGTTTCTAACCAAGGTTAGTTATGTCCACTTTCGTCATTTTACATTTTTATTTTTGTTTTATATATGTATTGAATTTTTTCATAATTTGGACTGTTTGTGAATTGCATTTTGGGAAGCCTGTTTTCTTGTGAAATTGTAACCATGGAGAAATTTTTAGATGATTCCAGTGCAACGAGGTCGGTAATTTTAGATGTAAGAGTGAGAAGTTACCTAGATTTGGTATCTAACGGTTATTAGGAGCAGACAAATTTGAAGTCATATATCTTTGGGGCCTTTTGTTTTGCCATAATCCAAGATTCTCTTAGGCATCTGGATTACCTCGTTTGTTTTGCGGTAATGTAAGATGTCCAGGCATTTAAAAAAATTTGGATGTCTGTCTGATACATGGGTTTCTAAGATTCCCATGTTAAGGGAATCGACCATTAACGTTTTGGTCTTTCGGTTTTATATTTTCTTTCATCAATTTTCTTTATTTCTTATTTTTTTTTTCCAATTTTAGGATTTTAAATAATTAGTGTATTCATTCACTAATAAACAATTATATGAAATTAACAATATTTATATGTTTCATTTACCTATAAAATAAATTCCATTTAATTTGTATAACATATAGTTGAATCTATTTTCAATTGAAAATTGAAGTGGTTTTTTTTTTTATTTATTTTACCTTTCCAATAACGATCATCTAATATCTTTTTTTTTTTTTTTTTTTTTTGAAAAAATCATCCAATATTTTTTTATTAAGAAAAATACAATCACAAATTAATATTATTTTCTCTCAAAACTTTCTCTATTTTGATAATTTATAAATATAATCCACTAAAAAATGCTATCAATTATATAATTGTTTTCTTTTTAAAATAACTTCAAGTAGTTCAAGTTTAAATTGAAATCAATATTTAATTCTAATTTTTTCATAAAAATCTCAAGACTATAAATTAATTTTTTTTTTTCTACAAAATAACATATAAAATTAATACAAATTGAATTGATAACAATATTAAATTATTAAAGTAAAAAATGTTATACAAATTAAATTATATTAAATTAATATATAAATGATTTGAAGATAGGAAATGATATTGTTGGAATAATACATCATTACATTCTCGTCCAGAAATCCTACAAAACAAACATGGTTATCTAAATATTCATGAACATGCCTATAAATTTTACTGCAAAACAAACATGGTTATCTAAATATGCCAGACATTTATATTTTCGGGCATATATAATTCTAGACATTTGAAAATTTCGACTATCTTTATTACCGAAAAACAAACGGTCCTTTATGGTTTTAAAGAGAGAGTCTCATTGCACTGGAAACATCTAATAATTCACTGTAATTGTGGTTGTGGTTAAAATATCAGTTTTGTGTGTAAAATTAAGGGTTTGTATGGTACCTTATGTTTTTCAAGAATGCATTTTTTTTAAAAAAAAATATTTTGCATAAAAATTGTTTAGAATTTAAATACTTATTTATTTATTTATTCCTCATAAGTGTTTTTATCCACAAGTCATTATGTATATAATTTTTTTTAAAAAAAAATTGTTAGTGTTTCACTTAAAATAGTTTATTTCTCAATCTCATTTTTTAATAAGTCATTTTTAATGGTTGCCAAACACTATAGTTTTTTTTTCAAAATGATTTTTTTTTCCTCAAAATTAAGCACTTGAAAAGTTAAACCAAACATATCCTAAAATGTATCGTTTTTATTAGATTGAAATATCATTTTGGTCTCTAAACTTTGAAACTTGTTTCGTTTTAGTCTTCATGCGTTCAAATGTTTAAATTTGATCCCATTACTTTCAATATATCTCAAAATCAGTTCCCACATTTCATTTATAGTTAAATTTTGGTAAAGATTTGGTAAACATAGTCACATTATGTCTTTTCTTAAATTAATTAATTTATTATTGGTCAGATAAAAATTAACTTCAAACTAACTATAAGGACTAATTTTAAGATATATTGAAAATACATAGACCAAATTTAAACGTTTGAAAGTAGAAGAACTAAAATGGAAGACAAGTAAACCGGGACCAAAATAACATTTTAACTTTATTATTTATGATTATTTTAAATTATATGTATTGAGATTTTTTTTTTTTTTAATTTTATTTGAGTTCAACAACATTTGGGGTGGAGGGATTCAAACTTCCAACCTCTTGATCGTGAGTACTTGACATACCGTTGAACTATGCTTACTTTAACATATGTATTAGGATTTGGGTCTTATTAGGAAACACTTATTTAATGAGGTCGTTCTAACGTGACTTTCTAATCTTAATAATAGTTGGATTATAAAATCTATCTGTGTTTCCTAATAAACACCAAATATAAATTTAAAGTAATTTTGAAGTTTTTTTTTTTTTCATACATCTAATGGTACGAAACTACTAGCCACATTGTGATGGTTTTCTTGAATATTTTGTTTTTGTGGAAATGATTGGGTTTGATTGGCCAGAGTTGTTCAGGTTCATCCATTCAGAATTTTGTAAAAGAAAGAAGGGAAAAAAGAAGTTGAAAATAATAGTCTTTTTATTTTTTTAGTATATTTTTTATAAATGATATATGGTGGTGTTACAAATTTTTGAAAAAAAAAACAATTTTTTATGCATGATAATTGAGGAGTTGATATAATTTATATTATGTCAGTATATTTAATTTTAAAAAAAGTTAGCATTTGAAATGTGTAAATATATATATTTTTTTATGATTTTTTCTTTGGGGAATTTAAATGTGTAGTTCCATTCTTTAAAAACAAATATATGTAACTATTTTTTTTTATATATAAAAAAAATACAAGTTTAACAGTTTGAAAAATAATTACAAATATCAGATATATTTCATTAAAACTTATAAACCATTGATTAGTTTAAATAAGGGTAGCTGTTATAATTTTTTTAGAGGTATTTGTATAAATAGCATTAAATGCATATGCATTTTTAAATATAGTAGAAACTCATAGAATTTTACCCAAAAAAAAAAAAAATTGCAATTCAGATAAGACTCAAATTCAAGTATTATAAAACACAATTGAAGAAAAGTCAAATGATACTATCTTTAAAAAAAAAAAAGAAAAAAAAATTGTGACATTTTCTTTTTTTAATTCCTGACATTTCTCTTTATTCTAATTAACCTACTTATTAACTAATGAACAATTGATTTGTAAATTCTAATTTGTAAACTTTTTAGCCTTTTTTTTTTTGGTATATCTGGATTATGTTTCTTTTAATAATAATTCTAAATTATATTAAATTAGGCCTATTTCTAATTGACAACAACATAACTCAACTGAAATAAAGTTCGTACTAATGATCGAGAGGTCTTTGGTTCGAATTTTCATCCTCACACATTATCAAAAAAAAAAAAAAAAAAAAAAACTTCTTCTAACCTTTTAATGAATACTATTGATGAAACATGCAAAGTTAACGTAAGTTTTTATTTTTATGGAGGATGGATAAATACGTCTCTATTGACTAGACATCATTAAGTTTTTGACACTAATTTACTTTTGTTTTTAAAATGATAAGTCCTCTCTTCCTTTTTATCGGTTAGCTGTCAAAAGTTTATAGTGGTTAATACTAGATGTATGAAGTCTTCCAATAAGTGATAAATACAATATTTGGAGTTTAGGAGTTCAATGAACGAAACTCTTGATGACATCAGAGCTGTCACTAAGCTTGTCACTTAAGTCAAACTTTCGAAGTTGGAAACACACTAAGTAAAGACTCTTTAGTTCGGTTTATAGTGGTTAACACTAGATGTGATGAAGTCTTTGGATAAGTGAGAACTACAATTAATACTGGAATTTAGAAGCTCAATGAATGAAACTCGATGTCACAGGACCTGTCTTTAAAGCTCGTCTCTTTTGTCGAGCTTACAACGTTGGAAACACACAATAAGTAAAAACTTGTCTTTTAAGTTGTGAACATTCTATATGTACTAAGAACTCCATTTGTTTTTGAATTGAAACAGATCTTCCATCCCAATGTAGAAAAAGATGGAACCATTTTCATTGACATATTACAAGAAAATTGGTCTTCAGCATTGACTGTTGAGAAACTTTTGCTTTCCATATGCTCAATTCTTTCAAGCCCAGATCATTATTTCTCCACTAATGAAGCTTGGCTCAGTTACACCAAGACTGCTAGAGAATGGACCAAAATATATGCAATGGGAAACTGATCTCTTCACTTAAATAGTTACCCCTTCTCTATTGTAAATTCATTTGGTTTTAGGGCTCTAGGTTATAAGAGGTTGGTTTTTGATATCTTGGTGAATGTTTTGTATTTTTTTATTTTGCCAAGCCCAACTTTGTATTTGGAATATGATACTTTGTATAATTGCCATTGTATAATGATTTTAGTTTGTATAATTGATAGTCTATTTGATTCAATGATGGTTAATTGGTCCGTCCATTTTTTTTTTTTTTGACAATATGTGGGGTGGGAGGATTCGAACCGTAGATCTCGTGGTCATCAGTATAATTTTATGCTAGTTGAGCTGTTTTCTTGTTGATTGGTCTGTTGATTTTGAGTTTTGAAAAGGCTGGTCAGGTTTTTAATAAATTTTGAAATAAGGTAGCTCAATATTTGAGTCAGCTAGATGACTTCACATCTTATGAATTAAGGAATACAAAGTATAAAAATTCAAATTATGAATAATGAATTTTCAAGATGTAACACCTCATCATCACATGTAGCCATGCCTCTTGTTCTGCTAAAATCTAG

mRNA sequence

ATGGTTCTTTCCCGTCTCCGCCGTTGCTTCTCTGCAAAATCCTCCTCTTCCACCGCCTACCTCCGCCTCCACACTCCGCCGCGCCCGGTTCCGATCGTCGACGAAGATTTCAAACGACCAGAGCCTGCGCCGCCGCGGCCGGTTCCGATCGTCGTCGTCGAGGATTTTAAACGGGCGGAGGATGCGCCGCCGCAGCCGATTCCGACCGTCGAGGGAGATGGTGACCGGAGAGAGGCCGCGTCGACGCGTCGTGTTACGATTAGCGAGGGCGGAAGCGGAAACGAAGAGAAAAGCAGTGCGATGTATCGGCTTGAGAACGAATTGAGAGCGATGGAGGAGGAGGCGCCGACGCACTGTAGCTTCGGGCCGGTCGGAGAGGACAGTTTCCGGTGGGAAGCGGCTGTAATTGGGCCGGCGTATTCTTGCTACGAAGGCGGTGTTTTTCACCTTTCCATTCAATTTCCCTCCGACTACCCCTTGAGGCCTCCCTGCATCAAGTTTCTAACCAAGGCATCTGGATTACCTCGTTTGTTTTGCGGTAATATCTTCCATCCCAATGTAGAAAAAGATGGAACCATTTTCATTGACATATTACAAGAAAATTGGTCTTCAGCATTGACTGTTGAGAAACTTTTGCTTTCCATATGCTCAATTCTTTCAAGCCCAGATCATTATTTCTCCACTAATGAAGCTTGGCTCAGTTACACCAAGACTGCTAGAGAATGGACCAAAATATATGCAATGGGAAACTGA

Coding sequence (CDS)

ATGGTTCTTTCCCGTCTCCGCCGTTGCTTCTCTGCAAAATCCTCCTCTTCCACCGCCTACCTCCGCCTCCACACTCCGCCGCGCCCGGTTCCGATCGTCGACGAAGATTTCAAACGACCAGAGCCTGCGCCGCCGCGGCCGGTTCCGATCGTCGTCGTCGAGGATTTTAAACGGGCGGAGGATGCGCCGCCGCAGCCGATTCCGACCGTCGAGGGAGATGGTGACCGGAGAGAGGCCGCGTCGACGCGTCGTGTTACGATTAGCGAGGGCGGAAGCGGAAACGAAGAGAAAAGCAGTGCGATGTATCGGCTTGAGAACGAATTGAGAGCGATGGAGGAGGAGGCGCCGACGCACTGTAGCTTCGGGCCGGTCGGAGAGGACAGTTTCCGGTGGGAAGCGGCTGTAATTGGGCCGGCGTATTCTTGCTACGAAGGCGGTGTTTTTCACCTTTCCATTCAATTTCCCTCCGACTACCCCTTGAGGCCTCCCTGCATCAAGTTTCTAACCAAGGCATCTGGATTACCTCGTTTGTTTTGCGGTAATATCTTCCATCCCAATGTAGAAAAAGATGGAACCATTTTCATTGACATATTACAAGAAAATTGGTCTTCAGCATTGACTGTTGAGAAACTTTTGCTTTCCATATGCTCAATTCTTTCAAGCCCAGATCATTATTTCTCCACTAATGAAGCTTGGCTCAGTTACACCAAGACTGCTAGAGAATGGACCAAAATATATGCAATGGGAAACTGA

Protein sequence

MVLSRLRRCFSAKSSSSTAYLRLHTPPRPVPIVDEDFKRPEPAPPRPVPIVVVEDFKRAEDAPPQPIPTVEGDGDRREAASTRRVTISEGGSGNEEKSSAMYRLENELRAMEEEAPTHCSFGPVGEDSFRWEAAVIGPAYSCYEGGVFHLSIQFPSDYPLRPPCIKFLTKASGLPRLFCGNIFHPNVEKDGTIFIDILQENWSSALTVEKLLLSICSILSSPDHYFSTNEAWLSYTKTAREWTKIYAMGN
Homology
BLAST of Spg023298 vs. NCBI nr
Match: XP_023000037.1 (ubiquitin-conjugating enzyme E2 E3-like [Cucurbita maxima])

HSP 1 Score: 282.3 bits (721), Expect = 4.0e-72
Identity = 170/315 (53.97%), Postives = 195/315 (61.90%), Query Frame = 0

Query: 1   MVLSRLRRCFSAKSSSSTAYLRLHTPP-------------------RPVPIVDEDFKRPE 60
           MVL RL  CFS KSSSST Y RLHTPP                    P+P VDE FK+PE
Sbjct: 1   MVLFRLCCCFSTKSSSSTVYTRLHTPPPPPLPIIHGDYAQPKPAQQLPIPFVDEGFKQPE 60

Query: 61  PAPPRPVPI-----------------VVVEDFKRAEDAPPQ-----------------PI 120
            +PP P+PI                 +V E FK+ ED+PP+                 PI
Sbjct: 61  ASPP-PLPIIHGDYGHPNPAQQLPIPIVDEVFKQPEDSPPRVPNIHGDFGQPKPAQKMPI 120

Query: 121 PTVEGDGDRREAASTRRVTISEG----GSGNEEKSSAMYRLENELRAMEEEAPTHCSFGP 180
           PT +    + EA+  R  T SEG    GSGNEEK+S+MYR+ENELRAM E A THCSFGP
Sbjct: 121 PTADEGFKQPEASPPRAGTNSEGDGGDGSGNEEKTSSMYRIENELRAMNEVAATHCSFGP 180

Query: 181 VGEDSFRWEAAVIGPAYSCYEGGVFHLSIQFPSDYPLRPPCIKFLTKASGLPRLFCGNIF 240
           VG D FRWE  VIGPA+SCY+GG+FHLSIQFPS+YPL PP IKFLTK           IF
Sbjct: 181 VGADIFRWEGVVIGPAFSCYDGGIFHLSIQFPSNYPLSPPLIKFLTK-----------IF 240

Query: 241 HPNVEKDGTIFIDILQENWSSALTVEKLLLSICSILSSPD-HYFSTNEA-------WLSY 251
           HPNVE+DGTI IDIL +NWS ALT+E L+LSICSILS+P+    S NEA       WL+Y
Sbjct: 241 HPNVEEDGTILIDILMDNWSPALTIETLMLSICSILSNPEPDEGSINEASRMFLNNWLNY 300

BLAST of Spg023298 vs. NCBI nr
Match: KAG6593782.1 (Ubiquitin-conjugating enzyme E2 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 274.2 bits (700), Expect = 1.1e-69
Identity = 167/315 (53.02%), Postives = 192/315 (60.95%), Query Frame = 0

Query: 1   MVLSRLRRCFSAKSSSSTAYLRLHTPP-------------------RPVPIVDEDFKRPE 60
           MVLSRL  CFS K SSST Y R HTPP                    P+PIVDE FK+PE
Sbjct: 1   MVLSRLCCCFSTKFSSSTVYTRFHTPPPPPVPIIHGDYAQPKPAQQLPIPIVDEGFKQPE 60

Query: 61  PAPPR-----------------PVPIVVVEDFKRAEDAPPQ-----------------PI 120
            +PPR                 P+PI V E  K+ ED+PP+                 PI
Sbjct: 61  ASPPRVPIIHGDYGHPNPAQQLPIPI-VDEVSKQPEDSPPRVPIMHGDLGQPMPAQHLPI 120

Query: 121 PTVEGDGDRREAASTRRVTISEG----GSGNEEKSSAMYRLENELRAMEEEAPTHCSFGP 180
           PT +    + EA+  R    S+G    GSGNEEK+SA+YR+ENELRAM E A THCSFGP
Sbjct: 121 PTADEGFKQPEASPPRAGGNSDGDGGDGSGNEEKTSAIYRIENELRAMNEAAATHCSFGP 180

Query: 181 VGEDSFRWEAAVIGPAYSCYEGGVFHLSIQFPSDYPLRPPCIKFLTKASGLPRLFCGNIF 240
           VG D  RWE  VIGPA+SCY+GG+FHLSIQFPS+YPL PP IKFLTK           IF
Sbjct: 181 VGADILRWEGIVIGPAFSCYDGGIFHLSIQFPSNYPLSPPLIKFLTK-----------IF 240

Query: 241 HPNVEKDGTIFIDILQENWSSALTVEKLLLSICSILSSPD-HYFSTNEA-------WLSY 251
           HPNVE+DGTI IDIL +NWS ALT+E L+LSICSILS+P+    S NEA       WL+Y
Sbjct: 241 HPNVEEDGTILIDILSDNWSPALTIETLMLSICSILSNPEPDEGSINEASRMFLNNWLNY 300

BLAST of Spg023298 vs. NCBI nr
Match: XP_023513655.1 (ubiquitin-conjugating enzyme E2 E3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 272.7 bits (696), Expect = 3.1e-69
Identity = 167/316 (52.85%), Postives = 195/316 (61.71%), Query Frame = 0

Query: 1   MVLSRLRRCFSAKSSSSTAYLRLHTPP-------------------RPVPIVDEDFKRPE 60
           MVLS L  CFS KSSSST Y RLHTPP                    P+PIVDE F++PE
Sbjct: 1   MVLSLLCCCFSTKSSSSTVYNRLHTPPPPPLPIIHGDYAQPKPAHQLPIPIVDEVFEQPE 60

Query: 61  PAPPR-----------------PVPIVVVEDFKRAEDAPPQ-----------------PI 120
            +PPR                 P+PI V E  K+ ED+PP+                 PI
Sbjct: 61  DSPPRVPIIHGDYGHPNPPQQLPIPI-VDEVSKQPEDSPPRVPIIHGDFGQPMPAQQLPI 120

Query: 121 PTVEGDGDRR-EAASTRRVTISEG----GSGNEEKSSAMYRLENELRAMEEEAPTHCSFG 180
           PT   +G ++ EA+  R    S+G    GSGNEEK+SAMYR+ENELRAM E A THCSFG
Sbjct: 121 PTAADEGFKQPEASPPRPGRNSDGDGGDGSGNEEKTSAMYRIENELRAMNEAAATHCSFG 180

Query: 181 PVGEDSFRWEAAVIGPAYSCYEGGVFHLSIQFPSDYPLRPPCIKFLTKASGLPRLFCGNI 240
           PVG D  RWE  VIGPA+SCY+GG+FHLSIQFPS+YPL PP IKFLTK           I
Sbjct: 181 PVGADILRWEGVVIGPAFSCYDGGIFHLSIQFPSNYPLSPPLIKFLTK-----------I 240

Query: 241 FHPNVEKDGTIFIDILQENWSSALTVEKLLLSICSILSSPD-HYFSTNEA-------WLS 251
           FHPN+++DGTI IDIL +NWS ALT+E L+LSICSILS+P+    S NEA       WL+
Sbjct: 241 FHPNIQEDGTILIDILTDNWSPALTIETLMLSICSILSNPEPDESSINEASRMYLDHWLN 300

BLAST of Spg023298 vs. NCBI nr
Match: XP_022964261.1 (ubiquitin-conjugating enzyme E2 E2-like [Cucurbita moschata])

HSP 1 Score: 270.8 bits (691), Expect = 1.2e-68
Identity = 167/316 (52.85%), Postives = 192/316 (60.76%), Query Frame = 0

Query: 1   MVLSRLRRCFSAKSSSSTAYLRLHTPP--------------------RPVPIVDEDFKRP 60
           MVLSRL  CFS KSSSST Y R HTPP                     P+PIVDE FK+P
Sbjct: 1   MVLSRLCCCFSTKSSSSTVYTRFHTPPPPPPVPIIHGDYAQPKPAQQLPIPIVDEGFKQP 60

Query: 61  EPAPPR-----------------PVPIVVVEDFKRAEDAPPQ-----------------P 120
           E +PPR                 P+PI V E  K+ ED+PP+                 P
Sbjct: 61  EASPPRVPIIHGDYGHLNPAQQLPIPI-VDEVSKQPEDSPPRVPIIHGDFGQPMPAQHLP 120

Query: 121 IPTVEGDGDRREAASTRRVTISEG----GSGNEEKSSAMYRLENELRAMEEEAPTHCSFG 180
           IPT +    + EA+  R    S+G    GSGNEEK+SAMYR+ENELRAM E A THCSFG
Sbjct: 121 IPTGDEGFKQPEASPPRAGGNSDGDGGDGSGNEEKTSAMYRIENELRAMNEAAATHCSFG 180

Query: 181 PVGEDSFRWEAAVIGPAYSCYEGGVFHLSIQFPSDYPLRPPCIKFLTKASGLPRLFCGNI 240
           PVG D  R E  VIGPA+SCY+GG+FHLSIQFPS+YPL PP IKFLTK           I
Sbjct: 181 PVGADILRCEGIVIGPAFSCYDGGIFHLSIQFPSNYPLSPPLIKFLTK-----------I 240

Query: 241 FHPNVEKDGTIFIDILQENWSSALTVEKLLLSICSILSSPD-HYFSTNEA-------WLS 251
           FHPN+E+DGTI IDIL +NWS ALT+E L+LSICSILS+P+    S NEA       WL+
Sbjct: 241 FHPNIEEDGTILIDILTDNWSPALTIETLMLSICSILSNPEPDEGSINEASRMFLNNWLN 300

BLAST of Spg023298 vs. NCBI nr
Match: XP_038907075.1 (ubiquitin-conjugating enzyme E2 D1-like [Benincasa hispida])

HSP 1 Score: 266.9 bits (681), Expect = 1.7e-67
Identity = 150/257 (58.37%), Postives = 169/257 (65.76%), Query Frame = 0

Query: 1   MVLSRLRRCFSAKSSSSTAYLRLHTPPRPVPIVDEDFKRPEPAPPRPVPIVVVEDFKRAE 60
           M  SRLRRCFS+  SSS+AY RL++PP+P+     D   P+P P                
Sbjct: 1   MFPSRLRRCFSSNPSSSSAYRRLYSPPQPI----SDTLSPQPNP---------------- 60

Query: 61  DAPPQPIPTVEGDGDRREAASTRRVTISEGGSGNEEKSSAMYRLENELRAMEEEAPTHCS 120
              P PI                 ++I +GGSGNEEKSSAMYR+ENELRAM EEA THCS
Sbjct: 61  --SPSPI---------------WPISIGDGGSGNEEKSSAMYRIENELRAMNEEAATHCS 120

Query: 121 FGPVGEDSFRWEAAVIGPAYSCYEGGVFHLSIQFPSDYPLRPPCIKFLTKASGLPRLFCG 180
           FGPVG+D FRWE  VIGPA SCYEGG+F LSIQFPS+YPLRPP IKFLTK          
Sbjct: 121 FGPVGDDIFRWEGVVIGPACSCYEGGIFQLSIQFPSNYPLRPPYIKFLTK---------- 180

Query: 181 NIFHPNVEKDGTIFIDILQENWSSALTVEKLLLSICSILSSPDHYFSTNEA-------WL 240
            IFHPNV  DGTIF+DIL ENWSSALT+EKLLLSICSILS+P    S NEA       WL
Sbjct: 181 -IFHPNVGSDGTIFMDILNENWSSALTIEKLLLSICSILSNPIPQGSINEASTMYLDNWL 209

Query: 241 SYTKTAREWTKIYAMGN 251
           +Y+K AREWTKIYAM N
Sbjct: 241 NYSKIAREWTKIYAMPN 209

BLAST of Spg023298 vs. ExPASy Swiss-Prot
Match: P35131 (Ubiquitin-conjugating enzyme E2 8 OS=Arabidopsis thaliana OX=3702 GN=UBC8 PE=1 SV=1)

HSP 1 Score: 149.1 bits (375), Expect = 6.9e-35
Identity = 73/160 (45.62%), Postives = 100/160 (62.50%), Query Frame = 0

Query: 100 AMYRLENELRAMEEEAPTHCSFGPVGEDSFRWEAAVIGPAYSCYEGGVFHLSIQFPSDYP 159
           A  R+  EL+ ++++ PT CS GPV ED F W+A ++GPA S Y GGVF ++I FP DYP
Sbjct: 2   ASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPAESPYSGGVFLVTIHFPPDYP 61

Query: 160 LRPPCIKFLTKASGLPRLFCGNIFHPNVEKDGTIFIDILQENWSSALTVEKLLLSICSIL 219
            +PP + F TK           +FHPN+  +G+I +DIL+E WS ALT+ K+LLSICS+L
Sbjct: 62  FKPPKVAFRTK-----------VFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLL 121

Query: 220 SSPD----------HYFSTNEAWLSYTKTAREWTKIYAMG 250
           + P+          H + T+ A   Y  TAR WT+ YAMG
Sbjct: 122 TDPNPDDPLVPEIAHMYKTDRA--KYEATARNWTQKYAMG 148

BLAST of Spg023298 vs. ExPASy Swiss-Prot
Match: Q94F47 (Ubiquitin-conjugating enzyme E2 28 OS=Arabidopsis thaliana OX=3702 GN=UBC28 PE=1 SV=1)

HSP 1 Score: 148.3 bits (373), Expect = 1.2e-34
Identity = 73/160 (45.62%), Postives = 100/160 (62.50%), Query Frame = 0

Query: 100 AMYRLENELRAMEEEAPTHCSFGPVGEDSFRWEAAVIGPAYSCYEGGVFHLSIQFPSDYP 159
           A  R+  EL+ ++++ PT CS GPV ED F W+A ++GP+ S Y GGVF ++I FP DYP
Sbjct: 2   ASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVTIHFPPDYP 61

Query: 160 LRPPCIKFLTKASGLPRLFCGNIFHPNVEKDGTIFIDILQENWSSALTVEKLLLSICSIL 219
            +PP + F TK           +FHPNV  +G+I +DIL+E WS ALT+ K+LLSICS+L
Sbjct: 62  FKPPKVAFRTK-----------VFHPNVNSNGSICLDILKEQWSPALTISKVLLSICSLL 121

Query: 220 SSPD----------HYFSTNEAWLSYTKTAREWTKIYAMG 250
           + P+          H + T+ A   Y  TAR WT+ YAMG
Sbjct: 122 TDPNPDDPLVPEIAHMYKTDRA--KYESTARSWTQKYAMG 148

BLAST of Spg023298 vs. ExPASy Swiss-Prot
Match: P35135 (Ubiquitin-conjugating enzyme E2-17 kDa OS=Solanum lycopersicum OX=4081 PE=2 SV=1)

HSP 1 Score: 147.9 bits (372), Expect = 1.5e-34
Identity = 73/160 (45.62%), Postives = 99/160 (61.88%), Query Frame = 0

Query: 100 AMYRLENELRAMEEEAPTHCSFGPVGEDSFRWEAAVIGPAYSCYEGGVFHLSIQFPSDYP 159
           A  R+  EL+ ++++ PT CS GPV ED F W+A ++GP  S Y GGVF +SI FP DYP
Sbjct: 2   ASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPTDSPYAGGVFLVSIHFPPDYP 61

Query: 160 LRPPCIKFLTKASGLPRLFCGNIFHPNVEKDGTIFIDILQENWSSALTVEKLLLSICSIL 219
            +PP + F TK           +FHPN+  +G+I +DIL+E WS ALT+ K+LLSICS+L
Sbjct: 62  FKPPKVAFRTK-----------VFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLL 121

Query: 220 SSPD----------HYFSTNEAWLSYTKTAREWTKIYAMG 250
           + P+          H + T+ A   Y  TAR WT+ YAMG
Sbjct: 122 TDPNPDDPLVPEIAHMYKTDRA--KYETTARSWTQKYAMG 148

BLAST of Spg023298 vs. ExPASy Swiss-Prot
Match: Q2TA10 (Ubiquitin-conjugating enzyme E2 D1 OS=Bos taurus OX=9913 GN=UBE2D1 PE=2 SV=1)

HSP 1 Score: 146.4 bits (368), Expect = 4.4e-34
Identity = 72/160 (45.00%), Postives = 98/160 (61.25%), Query Frame = 0

Query: 100 AMYRLENELRAMEEEAPTHCSFGPVGEDSFRWEAAVIGPAYSCYEGGVFHLSIQFPSDYP 159
           A+ R++ EL  ++ + P HCS GPVG+D F W+A ++GP  S Y+GGVF L++ FP+DYP
Sbjct: 2   ALKRIQKELSDLQRDPPAHCSAGPVGDDLFHWQATIMGPPDSAYQGGVFFLTVHFPTDYP 61

Query: 160 LRPPCIKFLTKASGLPRLFCGNIFHPNVEKDGTIFIDILQENWSSALTVEKLLLSICSIL 219
            +PP I F TK           I+HPN+  +G+I +DIL+  WS ALTV K+LLSICS+L
Sbjct: 62  FKPPKIAFTTK-----------IYHPNINSNGSICLDILRSQWSPALTVSKVLLSICSLL 121

Query: 220 SSPDH-----------YFSTNEAWLSYTKTAREWTKIYAM 249
             P+            Y S  E    Y + AREWT+ YAM
Sbjct: 122 CDPNPDDPLVPDIAQIYKSDKE---KYNRHAREWTQKYAM 147

BLAST of Spg023298 vs. ExPASy Swiss-Prot
Match: P51668 (Ubiquitin-conjugating enzyme E2 D1 OS=Homo sapiens OX=9606 GN=UBE2D1 PE=1 SV=1)

HSP 1 Score: 146.4 bits (368), Expect = 4.4e-34
Identity = 72/160 (45.00%), Postives = 98/160 (61.25%), Query Frame = 0

Query: 100 AMYRLENELRAMEEEAPTHCSFGPVGEDSFRWEAAVIGPAYSCYEGGVFHLSIQFPSDYP 159
           A+ R++ EL  ++ + P HCS GPVG+D F W+A ++GP  S Y+GGVF L++ FP+DYP
Sbjct: 2   ALKRIQKELSDLQRDPPAHCSAGPVGDDLFHWQATIMGPPDSAYQGGVFFLTVHFPTDYP 61

Query: 160 LRPPCIKFLTKASGLPRLFCGNIFHPNVEKDGTIFIDILQENWSSALTVEKLLLSICSIL 219
            +PP I F TK           I+HPN+  +G+I +DIL+  WS ALTV K+LLSICS+L
Sbjct: 62  FKPPKIAFTTK-----------IYHPNINSNGSICLDILRSQWSPALTVSKVLLSICSLL 121

Query: 220 SSPDH-----------YFSTNEAWLSYTKTAREWTKIYAM 249
             P+            Y S  E    Y + AREWT+ YAM
Sbjct: 122 CDPNPDDPLVPDIAQIYKSDKE---KYNRHAREWTQKYAM 147

BLAST of Spg023298 vs. ExPASy TrEMBL
Match: A0A6J1KCF2 (ubiquitin-conjugating enzyme E2 E3-like OS=Cucurbita maxima OX=3661 GN=LOC111494344 PE=4 SV=1)

HSP 1 Score: 282.3 bits (721), Expect = 1.9e-72
Identity = 170/315 (53.97%), Postives = 195/315 (61.90%), Query Frame = 0

Query: 1   MVLSRLRRCFSAKSSSSTAYLRLHTPP-------------------RPVPIVDEDFKRPE 60
           MVL RL  CFS KSSSST Y RLHTPP                    P+P VDE FK+PE
Sbjct: 1   MVLFRLCCCFSTKSSSSTVYTRLHTPPPPPLPIIHGDYAQPKPAQQLPIPFVDEGFKQPE 60

Query: 61  PAPPRPVPI-----------------VVVEDFKRAEDAPPQ-----------------PI 120
            +PP P+PI                 +V E FK+ ED+PP+                 PI
Sbjct: 61  ASPP-PLPIIHGDYGHPNPAQQLPIPIVDEVFKQPEDSPPRVPNIHGDFGQPKPAQKMPI 120

Query: 121 PTVEGDGDRREAASTRRVTISEG----GSGNEEKSSAMYRLENELRAMEEEAPTHCSFGP 180
           PT +    + EA+  R  T SEG    GSGNEEK+S+MYR+ENELRAM E A THCSFGP
Sbjct: 121 PTADEGFKQPEASPPRAGTNSEGDGGDGSGNEEKTSSMYRIENELRAMNEVAATHCSFGP 180

Query: 181 VGEDSFRWEAAVIGPAYSCYEGGVFHLSIQFPSDYPLRPPCIKFLTKASGLPRLFCGNIF 240
           VG D FRWE  VIGPA+SCY+GG+FHLSIQFPS+YPL PP IKFLTK           IF
Sbjct: 181 VGADIFRWEGVVIGPAFSCYDGGIFHLSIQFPSNYPLSPPLIKFLTK-----------IF 240

Query: 241 HPNVEKDGTIFIDILQENWSSALTVEKLLLSICSILSSPD-HYFSTNEA-------WLSY 251
           HPNVE+DGTI IDIL +NWS ALT+E L+LSICSILS+P+    S NEA       WL+Y
Sbjct: 241 HPNVEEDGTILIDILMDNWSPALTIETLMLSICSILSNPEPDEGSINEASRMFLNNWLNY 300

BLAST of Spg023298 vs. ExPASy TrEMBL
Match: A0A6J1HIF1 (ubiquitin-conjugating enzyme E2 E2-like OS=Cucurbita moschata OX=3662 GN=LOC111464331 PE=4 SV=1)

HSP 1 Score: 270.8 bits (691), Expect = 5.8e-69
Identity = 167/316 (52.85%), Postives = 192/316 (60.76%), Query Frame = 0

Query: 1   MVLSRLRRCFSAKSSSSTAYLRLHTPP--------------------RPVPIVDEDFKRP 60
           MVLSRL  CFS KSSSST Y R HTPP                     P+PIVDE FK+P
Sbjct: 1   MVLSRLCCCFSTKSSSSTVYTRFHTPPPPPPVPIIHGDYAQPKPAQQLPIPIVDEGFKQP 60

Query: 61  EPAPPR-----------------PVPIVVVEDFKRAEDAPPQ-----------------P 120
           E +PPR                 P+PI V E  K+ ED+PP+                 P
Sbjct: 61  EASPPRVPIIHGDYGHLNPAQQLPIPI-VDEVSKQPEDSPPRVPIIHGDFGQPMPAQHLP 120

Query: 121 IPTVEGDGDRREAASTRRVTISEG----GSGNEEKSSAMYRLENELRAMEEEAPTHCSFG 180
           IPT +    + EA+  R    S+G    GSGNEEK+SAMYR+ENELRAM E A THCSFG
Sbjct: 121 IPTGDEGFKQPEASPPRAGGNSDGDGGDGSGNEEKTSAMYRIENELRAMNEAAATHCSFG 180

Query: 181 PVGEDSFRWEAAVIGPAYSCYEGGVFHLSIQFPSDYPLRPPCIKFLTKASGLPRLFCGNI 240
           PVG D  R E  VIGPA+SCY+GG+FHLSIQFPS+YPL PP IKFLTK           I
Sbjct: 181 PVGADILRCEGIVIGPAFSCYDGGIFHLSIQFPSNYPLSPPLIKFLTK-----------I 240

Query: 241 FHPNVEKDGTIFIDILQENWSSALTVEKLLLSICSILSSPD-HYFSTNEA-------WLS 251
           FHPN+E+DGTI IDIL +NWS ALT+E L+LSICSILS+P+    S NEA       WL+
Sbjct: 241 FHPNIEEDGTILIDILTDNWSPALTIETLMLSICSILSNPEPDEGSINEASRMFLNNWLN 300

BLAST of Spg023298 vs. ExPASy TrEMBL
Match: A0A6J1CDR5 (ubiquitin-conjugating enzyme E2 5A-like OS=Momordica charantia OX=3673 GN=LOC111009786 PE=4 SV=1)

HSP 1 Score: 264.2 bits (674), Expect = 5.4e-67
Identity = 156/259 (60.23%), Postives = 179/259 (69.11%), Query Frame = 0

Query: 1   MVLSRLRRCFSAKSSSSTAYLRLHT--PPRPVPIVDEDFKRPEPAPPRPVPIVVVEDFKR 60
           MVLSRLRRCFS+KSSSS++Y RL +  PP P+PI    ++ P   PPRPVPI        
Sbjct: 1   MVLSRLRRCFSSKSSSSSSYSRLRSPPPPPPLPIYHGHYRPPPIPPPRPVPI-------- 60

Query: 61  AEDAPPQPIPTVEGDG--DRREAASTRRVTISEGGSGNEEKSSAMYRLENELRAMEEEAP 120
           A  A  QP  T  GDG   R E A  RRV+I  GGSG+EEK+SAMYR+ENEL+AM EE+ 
Sbjct: 61  AAGAYRQP-ETTTGDGACGRVEEAPPRRVSIG-GGSGDEEKTSAMYRIENELKAMNEESA 120

Query: 121 THCSFGPVGEDSFRWEAAVIGPAYSCYEGGVFHLSIQFPSDYPLRPPCIKFLTKASGLPR 180
           THCSFGPVG D FRWE  VIGPA+SCYE G+F LSI  PSDYP  PP IKF TK      
Sbjct: 121 THCSFGPVGGDIFRWEGLVIGPAHSCYEEGIFGLSIDLPSDYPSTPPQIKFQTK------ 180

Query: 181 LFCGNIFHPNVEKDGTIFIDILQENWSSALTVEKLLLSICSILSSPDHYFSTNEAWLSY- 240
                IFHPN+E+DGTI IDIL+ENWS ALT+EKLLLSICSILS+P+   S NEA + Y 
Sbjct: 181 -----IFHPNIEEDGTIHIDILKENWSPALTIEKLLLSICSILSNPEAQNSINEASMMYK 238

Query: 241 ------TKTAREWTKIYAM 249
                  + AREWTKIYAM
Sbjct: 241 NEYFRFCEVAREWTKIYAM 238

BLAST of Spg023298 vs. ExPASy TrEMBL
Match: A0A0A0LI75 (UBC core domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G138770 PE=4 SV=1)

HSP 1 Score: 213.8 bits (543), Expect = 8.4e-52
Identity = 129/258 (50.00%), Postives = 148/258 (57.36%), Query Frame = 0

Query: 1   MVLSRLRRCFSAKSSSSTAYLRLHTPPRPVPIVDEDFKRPEPAPPRPVPIVVVEDFKRAE 60
           M  SRLRRCFS K S        +TP  P                 PV +     + R  
Sbjct: 1   MFFSRLRRCFSQKPSP-------YTPTSP-----------------PVRMEEKTSWTRGI 60

Query: 61  DAPPQPIPTVEGDGDRREAASTRRVTISEGGSGNEEKSS-AMYRLENELRAMEEEAPTHC 120
           D   + I                          NEEKSS AMYR+ENEL++M EE  THC
Sbjct: 61  DHQKEDI----------------------SWGNNEEKSSVAMYRIENELKSMNEEVATHC 120

Query: 121 SFGPVGEDSFRWEAAVIGPAYSCYEGGVFHLSIQFPSDYPLRPPCIKFLTKASGLPRLFC 180
           SFGPVG+D FRWE  VIGPA SCYEGG+FH+SIQFPSDYP  PP I FLTK         
Sbjct: 121 SFGPVGDDIFRWEGVVIGPAGSCYEGGIFHVSIQFPSDYPFTPPSINFLTK--------- 180

Query: 181 GNIFHPNVEKDGTIFIDILQENWSSALTVEKLLLSICSILSSPDHYFSTNEA-------W 240
             IFHPNVE DG+I +DIL ENWSSALT+EKLLLSICSILS+P  + STNEA       W
Sbjct: 181 --IFHPNVEVDGSIGMDILNENWSSALTIEKLLLSICSILSNPIPHGSTNEASTMFLNNW 201

Query: 241 LSYTKTAREWTKIYAMGN 251
           L +  TA++WTK+YAM N
Sbjct: 241 LDFNDTAKQWTKLYAMPN 201

BLAST of Spg023298 vs. ExPASy TrEMBL
Match: A0A1S3C6C4 (ubiquitin-conjugating enzyme E2 D1-like OS=Cucumis melo OX=3656 GN=LOC103496968 PE=4 SV=1)

HSP 1 Score: 213.0 bits (541), Expect = 1.4e-51
Identity = 115/199 (57.79%), Postives = 136/199 (68.34%), Query Frame = 0

Query: 64  PQPIPTVE------GDGDRREAASTRRVTISEGGSGNEEKSS-AMYRLENELRAMEEEAP 123
           P P+P ++      G+ D+++    R          NEEKSS AMYR+ENEL+AM +E  
Sbjct: 23  PPPVPMIKKTSWPCGNIDQKDDDIIR--------GNNEEKSSVAMYRIENELKAMNKEVA 82

Query: 124 THCSFGPVGEDSFRWEAAVIGPAYSCYEGGVFHLSIQFPSDYPLRPPCIKFLTKASGLPR 183
           THCSFGPVG+D FRWE  VIGPA SCYEGG+FH+SIQFPSDYP  PP I FLTK      
Sbjct: 83  THCSFGPVGDDIFRWEGIVIGPACSCYEGGIFHVSIQFPSDYPFTPPSIHFLTK------ 142

Query: 184 LFCGNIFHPNVEKDGTIFIDILQENWSSALTVEKLLLSICSILSSPDHYFSTNEA----- 243
                IFHPNVE DG+I IDIL ENWSSALT+EKLLLSICS+LS+P    STNEA     
Sbjct: 143 -----IFHPNVELDGSIKIDILYENWSSALTIEKLLLSICSVLSNPIPQGSTNEASTMFL 202

Query: 244 --WLSYTKTAREWTKIYAM 249
             WL++   AR+WTK+YAM
Sbjct: 203 NDWLNFNDIARQWTKLYAM 202

BLAST of Spg023298 vs. TAIR 10
Match: AT5G41700.2 (ubiquitin conjugating enzyme 8 )

HSP 1 Score: 149.1 bits (375), Expect = 4.9e-36
Identity = 73/160 (45.62%), Postives = 100/160 (62.50%), Query Frame = 0

Query: 100 AMYRLENELRAMEEEAPTHCSFGPVGEDSFRWEAAVIGPAYSCYEGGVFHLSIQFPSDYP 159
           A  R+  EL+ ++++ PT CS GPV ED F W+A ++GPA S Y GGVF ++I FP DYP
Sbjct: 2   ASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPAESPYSGGVFLVTIHFPPDYP 61

Query: 160 LRPPCIKFLTKASGLPRLFCGNIFHPNVEKDGTIFIDILQENWSSALTVEKLLLSICSIL 219
            +PP + F TK           +FHPN+  +G+I +DIL+E WS ALT+ K+LLSICS+L
Sbjct: 62  FKPPKVAFRTK-----------VFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLL 121

Query: 220 SSPD----------HYFSTNEAWLSYTKTAREWTKIYAMG 250
           + P+          H + T+ A   Y  TAR WT+ YAMG
Sbjct: 122 TDPNPDDPLVPEIAHMYKTDRA--KYEATARNWTQKYAMG 148

BLAST of Spg023298 vs. TAIR 10
Match: AT5G41700.1 (ubiquitin conjugating enzyme 8 )

HSP 1 Score: 149.1 bits (375), Expect = 4.9e-36
Identity = 73/160 (45.62%), Postives = 100/160 (62.50%), Query Frame = 0

Query: 100 AMYRLENELRAMEEEAPTHCSFGPVGEDSFRWEAAVIGPAYSCYEGGVFHLSIQFPSDYP 159
           A  R+  EL+ ++++ PT CS GPV ED F W+A ++GPA S Y GGVF ++I FP DYP
Sbjct: 2   ASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPAESPYSGGVFLVTIHFPPDYP 61

Query: 160 LRPPCIKFLTKASGLPRLFCGNIFHPNVEKDGTIFIDILQENWSSALTVEKLLLSICSIL 219
            +PP + F TK           +FHPN+  +G+I +DIL+E WS ALT+ K+LLSICS+L
Sbjct: 62  FKPPKVAFRTK-----------VFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLL 121

Query: 220 SSPD----------HYFSTNEAWLSYTKTAREWTKIYAMG 250
           + P+          H + T+ A   Y  TAR WT+ YAMG
Sbjct: 122 TDPNPDDPLVPEIAHMYKTDRA--KYEATARNWTQKYAMG 148

BLAST of Spg023298 vs. TAIR 10
Match: AT5G41700.5 (ubiquitin conjugating enzyme 8 )

HSP 1 Score: 149.1 bits (375), Expect = 4.9e-36
Identity = 73/160 (45.62%), Postives = 100/160 (62.50%), Query Frame = 0

Query: 100 AMYRLENELRAMEEEAPTHCSFGPVGEDSFRWEAAVIGPAYSCYEGGVFHLSIQFPSDYP 159
           A  R+  EL+ ++++ PT CS GPV ED F W+A ++GPA S Y GGVF ++I FP DYP
Sbjct: 2   ASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPAESPYSGGVFLVTIHFPPDYP 61

Query: 160 LRPPCIKFLTKASGLPRLFCGNIFHPNVEKDGTIFIDILQENWSSALTVEKLLLSICSIL 219
            +PP + F TK           +FHPN+  +G+I +DIL+E WS ALT+ K+LLSICS+L
Sbjct: 62  FKPPKVAFRTK-----------VFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLL 121

Query: 220 SSPD----------HYFSTNEAWLSYTKTAREWTKIYAMG 250
           + P+          H + T+ A   Y  TAR WT+ YAMG
Sbjct: 122 TDPNPDDPLVPEIAHMYKTDRA--KYEATARNWTQKYAMG 148

BLAST of Spg023298 vs. TAIR 10
Match: AT1G64230.1 (ubiquitin-conjugating enzyme 28 )

HSP 1 Score: 148.3 bits (373), Expect = 8.3e-36
Identity = 73/160 (45.62%), Postives = 100/160 (62.50%), Query Frame = 0

Query: 100 AMYRLENELRAMEEEAPTHCSFGPVGEDSFRWEAAVIGPAYSCYEGGVFHLSIQFPSDYP 159
           A  R+  EL+ ++++ PT CS GPV ED F W+A ++GP+ S Y GGVF ++I FP DYP
Sbjct: 2   ASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVTIHFPPDYP 61

Query: 160 LRPPCIKFLTKASGLPRLFCGNIFHPNVEKDGTIFIDILQENWSSALTVEKLLLSICSIL 219
            +PP + F TK           +FHPNV  +G+I +DIL+E WS ALT+ K+LLSICS+L
Sbjct: 62  FKPPKVAFRTK-----------VFHPNVNSNGSICLDILKEQWSPALTISKVLLSICSLL 121

Query: 220 SSPD----------HYFSTNEAWLSYTKTAREWTKIYAMG 250
           + P+          H + T+ A   Y  TAR WT+ YAMG
Sbjct: 122 TDPNPDDPLVPEIAHMYKTDRA--KYESTARSWTQKYAMG 148

BLAST of Spg023298 vs. TAIR 10
Match: AT1G64230.2 (ubiquitin-conjugating enzyme 28 )

HSP 1 Score: 148.3 bits (373), Expect = 8.3e-36
Identity = 73/160 (45.62%), Postives = 100/160 (62.50%), Query Frame = 0

Query: 100 AMYRLENELRAMEEEAPTHCSFGPVGEDSFRWEAAVIGPAYSCYEGGVFHLSIQFPSDYP 159
           A  R+  EL+ ++++ PT CS GPV ED F W+A ++GP+ S Y GGVF ++I FP DYP
Sbjct: 2   ASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVTIHFPPDYP 61

Query: 160 LRPPCIKFLTKASGLPRLFCGNIFHPNVEKDGTIFIDILQENWSSALTVEKLLLSICSIL 219
            +PP + F TK           +FHPNV  +G+I +DIL+E WS ALT+ K+LLSICS+L
Sbjct: 62  FKPPKVAFRTK-----------VFHPNVNSNGSICLDILKEQWSPALTISKVLLSICSLL 121

Query: 220 SSPD----------HYFSTNEAWLSYTKTAREWTKIYAMG 250
           + P+          H + T+ A   Y  TAR WT+ YAMG
Sbjct: 122 TDPNPDDPLVPEIAHMYKTDRA--KYESTARSWTQKYAMG 148

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023000037.14.0e-7253.97ubiquitin-conjugating enzyme E2 E3-like [Cucurbita maxima][more]
KAG6593782.11.1e-6953.02Ubiquitin-conjugating enzyme E2 2, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_023513655.13.1e-6952.85ubiquitin-conjugating enzyme E2 E3-like [Cucurbita pepo subsp. pepo][more]
XP_022964261.11.2e-6852.85ubiquitin-conjugating enzyme E2 E2-like [Cucurbita moschata][more]
XP_038907075.11.7e-6758.37ubiquitin-conjugating enzyme E2 D1-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
P351316.9e-3545.63Ubiquitin-conjugating enzyme E2 8 OS=Arabidopsis thaliana OX=3702 GN=UBC8 PE=1 S... [more]
Q94F471.2e-3445.63Ubiquitin-conjugating enzyme E2 28 OS=Arabidopsis thaliana OX=3702 GN=UBC28 PE=1... [more]
P351351.5e-3445.63Ubiquitin-conjugating enzyme E2-17 kDa OS=Solanum lycopersicum OX=4081 PE=2 SV=1[more]
Q2TA104.4e-3445.00Ubiquitin-conjugating enzyme E2 D1 OS=Bos taurus OX=9913 GN=UBE2D1 PE=2 SV=1[more]
P516684.4e-3445.00Ubiquitin-conjugating enzyme E2 D1 OS=Homo sapiens OX=9606 GN=UBE2D1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1KCF21.9e-7253.97ubiquitin-conjugating enzyme E2 E3-like OS=Cucurbita maxima OX=3661 GN=LOC111494... [more]
A0A6J1HIF15.8e-6952.85ubiquitin-conjugating enzyme E2 E2-like OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A6J1CDR55.4e-6760.23ubiquitin-conjugating enzyme E2 5A-like OS=Momordica charantia OX=3673 GN=LOC111... [more]
A0A0A0LI758.4e-5250.00UBC core domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G138770 PE... [more]
A0A1S3C6C41.4e-5157.79ubiquitin-conjugating enzyme E2 D1-like OS=Cucumis melo OX=3656 GN=LOC103496968 ... [more]
Match NameE-valueIdentityDescription
AT5G41700.24.9e-3645.63ubiquitin conjugating enzyme 8 [more]
AT5G41700.14.9e-3645.63ubiquitin conjugating enzyme 8 [more]
AT5G41700.54.9e-3645.63ubiquitin conjugating enzyme 8 [more]
AT1G64230.18.3e-3645.63ubiquitin-conjugating enzyme 28 [more]
AT1G64230.28.3e-3645.63ubiquitin-conjugating enzyme 28 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 94..114
NoneNo IPR availableSMARTSM00212ubc_7coord: 102..248
e-value: 3.0E-36
score: 136.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 13..98
NoneNo IPR availablePANTHERPTHR24068UBIQUITIN-CONJUGATING ENZYME E2coord: 102..248
NoneNo IPR availablePANTHERPTHR24068:SF242UBIQUITIN CARRIER PROTEINcoord: 102..248
IPR016135Ubiquitin-conjugating enzyme/RWD-likeGENE3D3.10.110.10Ubiquitin Conjugating Enzymecoord: 91..250
e-value: 1.1E-44
score: 153.8
IPR016135Ubiquitin-conjugating enzyme/RWD-likeSUPERFAMILY54495UBC-likecoord: 93..248
IPR000608Ubiquitin-conjugating enzyme E2PFAMPF00179UQ_concoord: 103..232
e-value: 7.8E-35
score: 119.6
IPR000608Ubiquitin-conjugating enzyme E2PROSITEPS50127UBC_2coord: 99..250
score: 37.159492
IPR000608Ubiquitin-conjugating enzyme E2CDDcd00195UBCccoord: 103..243
e-value: 1.31851E-41
score: 137.334

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg023298.1Spg023298.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0044267 cellular protein metabolic process
molecular_function GO:0003824 catalytic activity