Spg022821 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg022821 vs. NCBI nr
Match: KAG6592918.1 (Pentatricopeptide repeat-containing protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 402.9 bits (1034), Expect = 2.3e-108
Identity = 234/443 (52.82%), Postives = 253/443 (57.11%), Query Frame = 0

Query: 1   MAFCADPSLGNLRYAERMFDYVQDPSLFVYNVMVKAYNKR-------------------- 60
           MAFCADPS+GNLRY ERMFDY+Q PSLFVYNVMVKAY KR                    
Sbjct: 74  MAFCADPSVGNLRYTERMFDYIQGPSLFVYNVMVKAYAKRGIFRKVLLLFQRLREDELWP 133

Query: 61  --------------------GEKIHVFVVKTGMDYDDYVCNSLMDMYAELSKIGNAKKLF 120
                               GEK+H FVVKTGM  D YVCNSLMDMYAEL K+GNAKKLF
Sbjct: 134 DNFTYPFVLKAIGCLRDARKGEKVHGFVVKTGMVCDTYVCNSLMDMYAELGKVGNAKKLF 193

Query: 121 DEMPTRDYVSWNVMIAGYVRCRRFEDALNTFKEMQQESNEKPSEAAVVSTLSAF------ 180
           DEMP RD VSWNV+I+GYVRCRRFEDA+N FKEMQQESNEKP EA VVSTLSA       
Sbjct: 194 DEMPRRDSVSWNVLISGYVRCRRFEDAVNVFKEMQQESNEKPGEATVVSTLSACTALKNL 253

Query: 181 -----LHDYVRKELGFTTIINNALLDMCTK------------------------------ 240
                +H+YVRK+LGFTTIINNALLDM  K                              
Sbjct: 254 ELGVEIHNYVRKQLGFTTIINNALLDMYAKCGCLDIARKIFDEMPMRNVICWTSMISSYI 313

Query: 241 -----------FGSSRAREMDSW------------------------------------- 286
                      F  S  R++  W                                     
Sbjct: 314 NCGDLTEARDLFDRSPVRDVVLWTAMINGYVQFNHFDEAVALFREMQFQKVKPDKFTVVA 373

BLAST of Spg022821 vs. NCBI nr
Match: KAG7025324.1 (Pentatricopeptide repeat-containing protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 402.9 bits (1034), Expect = 2.3e-108
Identity = 234/443 (52.82%), Postives = 253/443 (57.11%), Query Frame = 0

Query: 1   MAFCADPSLGNLRYAERMFDYVQDPSLFVYNVMVKAYNKR-------------------- 60
           MAFCADPS+GNLRY ERMFDY+Q PSLFVYNVMVKAY KR                    
Sbjct: 81  MAFCADPSVGNLRYTERMFDYIQGPSLFVYNVMVKAYAKRGIFRKVLLLFQRLREDELWP 140

Query: 61  --------------------GEKIHVFVVKTGMDYDDYVCNSLMDMYAELSKIGNAKKLF 120
                               GEK+H FVVKTGM  D YVCNSLMDMYAEL K+GNAKKLF
Sbjct: 141 DNFTYPFVLKAIGCLRDARKGEKVHGFVVKTGMVCDTYVCNSLMDMYAELGKVGNAKKLF 200

Query: 121 DEMPTRDYVSWNVMIAGYVRCRRFEDALNTFKEMQQESNEKPSEAAVVSTLSAF------ 180
           DEMP RD VSWNV+I+GYVRCRRFEDA+N FKEMQQESNEKP EA VVSTLSA       
Sbjct: 201 DEMPRRDSVSWNVLISGYVRCRRFEDAVNVFKEMQQESNEKPGEATVVSTLSACTALKNL 260

Query: 181 -----LHDYVRKELGFTTIINNALLDMCTK------------------------------ 240
                +H+YVRK+LGFTTIINNALLDM  K                              
Sbjct: 261 ELGAGIHNYVRKQLGFTTIINNALLDMYAKCGCLDIARKIFDEMPMRNVICWTSMISSYI 320

Query: 241 -----------FGSSRAREMDSW------------------------------------- 286
                      F  S  R++  W                                     
Sbjct: 321 NCGDLTEARDLFDRSPVRDVVLWTAMINGYVQFNHFDEAVALFREMQFQKVKPDKFTVVA 380

BLAST of Spg022821 vs. NCBI nr
Match: XP_023513900.1 (pentatricopeptide repeat-containing protein At1g31430 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023513902.1 pentatricopeptide repeat-containing protein At1g31430 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 402.9 bits (1034), Expect = 2.3e-108
Identity = 234/443 (52.82%), Postives = 253/443 (57.11%), Query Frame = 0

Query: 1   MAFCADPSLGNLRYAERMFDYVQDPSLFVYNVMVKAYNKR-------------------- 60
           MAFCADPS+GNLRY E+MFDY+Q PSLFVYNVMVKAY KR                    
Sbjct: 81  MAFCADPSVGNLRYTEKMFDYIQGPSLFVYNVMVKAYAKRGSFRKVLLLFQRLREDELWP 140

Query: 61  --------------------GEKIHVFVVKTGMDYDDYVCNSLMDMYAELSKIGNAKKLF 120
                               GEK+H FVVKTGM  D YVCNSLMDMYAEL K+GNAKKLF
Sbjct: 141 DNFTYPFVLKAIGCLRDARKGEKVHGFVVKTGMVCDTYVCNSLMDMYAELGKVGNAKKLF 200

Query: 121 DEMPTRDYVSWNVMIAGYVRCRRFEDALNTFKEMQQESNEKPSEAAVVSTLSAF------ 180
           DEMP RD VSWNV+I+GYVRCRRFEDA+N FKEMQQESNEKP EA VVSTLSA       
Sbjct: 201 DEMPRRDSVSWNVLISGYVRCRRFEDAVNVFKEMQQESNEKPGEATVVSTLSACTALKNL 260

Query: 181 -----LHDYVRKELGFTTIINNALLDMCTK------------------------------ 240
                +H+YVRKELGFTTIINNALLDM  K                              
Sbjct: 261 ELGVEIHNYVRKELGFTTIINNALLDMYAKCGCLDIARKIFDEMPMRNVICWTSMISSYI 320

Query: 241 -----------FGSSRAREMDSW------------------------------------- 286
                      F  S  R++  W                                     
Sbjct: 321 NCGDLTEARDLFDRSPVRDVVLWTAMINGYVQFNHFDEAVALFREMQFQKVKPDKFTVVA 380

BLAST of Spg022821 vs. NCBI nr
Match: XP_022960435.1 (pentatricopeptide repeat-containing protein At1g31430 [Cucurbita moschata] >XP_022960436.1 pentatricopeptide repeat-containing protein At1g31430 [Cucurbita moschata] >XP_022960437.1 pentatricopeptide repeat-containing protein At1g31430 [Cucurbita moschata] >XP_022960438.1 pentatricopeptide repeat-containing protein At1g31430 [Cucurbita moschata])

HSP 1 Score: 402.9 bits (1034), Expect = 2.3e-108
Identity = 234/443 (52.82%), Postives = 253/443 (57.11%), Query Frame = 0

Query: 1   MAFCADPSLGNLRYAERMFDYVQDPSLFVYNVMVKAYNKR-------------------- 60
           MAFCADPS+GNLRY ERMFDY+Q PSLFVYNVMVKAY KR                    
Sbjct: 81  MAFCADPSVGNLRYTERMFDYIQGPSLFVYNVMVKAYAKRGIFRKVLLLFQRLREDELWP 140

Query: 61  --------------------GEKIHVFVVKTGMDYDDYVCNSLMDMYAELSKIGNAKKLF 120
                               GEK+H FVVKTGM  D YVCNSLMDMYAEL K+GNAKKLF
Sbjct: 141 DNFTYPFVLKAIGCLRDARKGEKVHGFVVKTGMVCDTYVCNSLMDMYAELGKVGNAKKLF 200

Query: 121 DEMPTRDYVSWNVMIAGYVRCRRFEDALNTFKEMQQESNEKPSEAAVVSTLSAF------ 180
           DEMP RD VSWNV+I+GYVRCRRFEDA+N FKEMQQESNEKP EA VVSTLSA       
Sbjct: 201 DEMPRRDSVSWNVLISGYVRCRRFEDAVNVFKEMQQESNEKPGEATVVSTLSACTALKNL 260

Query: 181 -----LHDYVRKELGFTTIINNALLDMCTK------------------------------ 240
                +H+YVRK+LGFTTIINNALLDM  K                              
Sbjct: 261 ELGVEIHNYVRKQLGFTTIINNALLDMYAKCGCLDIARKIFDEMPMRNVICWTSMISSYI 320

Query: 241 -----------FGSSRAREMDSW------------------------------------- 286
                      F  S  R++  W                                     
Sbjct: 321 NCGDLTEARDLFDRSPVRDVVLWTAMINGYVQFNHFDEAVALFREMQFQKVKPDKFTVVA 380

BLAST of Spg022821 vs. NCBI nr
Match: XP_023513901.1 (pentatricopeptide repeat-containing protein At1g31430 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 402.9 bits (1034), Expect = 2.3e-108
Identity = 234/443 (52.82%), Postives = 253/443 (57.11%), Query Frame = 0

Query: 1   MAFCADPSLGNLRYAERMFDYVQDPSLFVYNVMVKAYNKR-------------------- 60
           MAFCADPS+GNLRY E+MFDY+Q PSLFVYNVMVKAY KR                    
Sbjct: 1   MAFCADPSVGNLRYTEKMFDYIQGPSLFVYNVMVKAYAKRGSFRKVLLLFQRLREDELWP 60

Query: 61  --------------------GEKIHVFVVKTGMDYDDYVCNSLMDMYAELSKIGNAKKLF 120
                               GEK+H FVVKTGM  D YVCNSLMDMYAEL K+GNAKKLF
Sbjct: 61  DNFTYPFVLKAIGCLRDARKGEKVHGFVVKTGMVCDTYVCNSLMDMYAELGKVGNAKKLF 120

Query: 121 DEMPTRDYVSWNVMIAGYVRCRRFEDALNTFKEMQQESNEKPSEAAVVSTLSAF------ 180
           DEMP RD VSWNV+I+GYVRCRRFEDA+N FKEMQQESNEKP EA VVSTLSA       
Sbjct: 121 DEMPRRDSVSWNVLISGYVRCRRFEDAVNVFKEMQQESNEKPGEATVVSTLSACTALKNL 180

Query: 181 -----LHDYVRKELGFTTIINNALLDMCTK------------------------------ 240
                +H+YVRKELGFTTIINNALLDM  K                              
Sbjct: 181 ELGVEIHNYVRKELGFTTIINNALLDMYAKCGCLDIARKIFDEMPMRNVICWTSMISSYI 240

Query: 241 -----------FGSSRAREMDSW------------------------------------- 286
                      F  S  R++  W                                     
Sbjct: 241 NCGDLTEARDLFDRSPVRDVVLWTAMINGYVQFNHFDEAVALFREMQFQKVKPDKFTVVA 300

BLAST of Spg022821 vs. ExPASy Swiss-Prot
Match: Q9C866 (Pentatricopeptide repeat-containing protein At1g31430 OS=Arabidopsis thaliana OX=3702 GN=PCMP-E55 PE=2 SV=1)

HSP 1 Score: 218.0 bits (554), Expect = 1.4e-55
Identity = 138/383 (36.03%), Postives = 180/383 (47.00%), Query Frame = 0

Query: 25  PSLFVYNVMVKAYNK-----RGEKIHVFVVKTGMDYDDYVCNSLMDMYAELSKIGNAKKL 84
           P  F   V++K+  +      GEK+H + VK G+++D YV NSLM MYA L KI    K+
Sbjct: 44  PDNFTLPVVLKSIGRLRKVIEGEKVHGYAVKAGLEFDSYVSNSLMGMYASLGKIEITHKV 103

Query: 85  FDEMPTRDYVSWNVMIAGYVRCRRFEDALNTFKEMQQESNEKPSEAAVVSTLSAF----- 144
           FDEMP RD VSWN +I+ YV   RFEDA+  FK M QESN K  E  +VSTLSA      
Sbjct: 104 FDEMPQRDVVSWNGLISSYVGNGRFEDAIGVFKRMSQESNLKFDEGTIVSTLSACSALKN 163

Query: 145 ------LHDYVRKELGFTTIINNALLDMCTK----------FGSSRAREMDSW------- 204
                 ++ +V  E   +  I NAL+DM  K          F S R + +  W       
Sbjct: 164 LEIGERIYRFVVTEFEMSVRIGNALVDMFCKCGCLDKARAVFDSMRDKNVKCWTSMVFGY 223

Query: 205 ------------------------------------------------------------ 264
                                                                       
Sbjct: 224 VSTGRIDEARVLFERSPVKDVVLWTAMMNGYVQFNRFDEALELFRCMQTAGIRPDNFVLV 283

Query: 265 --------------------------ITMDAVVGTALIEMYSKCGCVDKSLEIFCELKEK 285
                                     +T+D VVGTAL++MY+KCGC++ +LE+F E+KE+
Sbjct: 284 SLLTGCAQTGALEQGKWIHGYINENRVTVDKVVGTALVDMYAKCGCIETALEVFYEIKER 343

BLAST of Spg022821 vs. ExPASy Swiss-Prot
Match: Q9FG16 (Pentatricopeptide repeat-containing protein At5g06540 OS=Arabidopsis thaliana OX=3702 GN=PCMP-H88 PE=3 SV=1)

HSP 1 Score: 174.9 bits (442), Expect = 1.3e-42
Identity = 109/349 (31.23%), Postives = 176/349 (50.43%), Query Frame = 0

Query: 1   MAFCADPSLGN-----LRYAERMFDYVQDPSLFVYNVMVKAYNKR--------------- 60
           +A C D S  N     L YA  +F  +Q+P+LFV+N++++ ++                 
Sbjct: 51  LALCVDDSTFNKPTNLLGYAYGIFSQIQNPNLFVFNLLIRCFSTGAEPSKAFGFYTQMLK 110

Query: 61  -------------------------GEKIHVFVVKTGMDYDDYVCNSLMDMYAELSKIGN 120
                                    GE+ H  +V+ G   D YV NSL+ MYA    I  
Sbjct: 111 SRIWPDNITFPFLIKASSEMECVLVGEQTHSQIVRFGFQNDVYVENSLVHMYANCGFIAA 170

Query: 121 AKKLFDEMPTRDYVSWNVMIAGYVRCRRFEDALNTFKEMQQES--------NEKPSEAAV 180
           A ++F +M  RD VSW  M+AGY +C   E+A   F EM   +        N        
Sbjct: 171 AGRIFGQMGFRDVVSWTSMVAGYCKCGMVENAREMFDEMPHRNLFTWSIMINGYAKNNCF 230

Query: 181 VSTLSAFLHDYVRKE-LGFTTIINNALLDMCTKFGS----SRARE--MDSWITMDAVVGT 240
              +  F  +++++E +     +  +++  C   G+     RA E  + S +T++ ++GT
Sbjct: 231 EKAIDLF--EFMKREGVVANETVMVSVISSCAHLGALEFGERAYEYVVKSHMTVNLILGT 290

Query: 241 ALIEMYSKCGCVDKSLEIFCELKEKDTTSWTSIICGLAMNGKTGETPRLFTAMELVRARL 286
           AL++M+ +CG ++K++ +F  L E D+ SW+SII GLA++G   +    F+ M  +    
Sbjct: 291 ALVDMFWRCGDIEKAIHVFEGLPETDSLSWSSIIKGLAVHGHAHKAMHYFSQMISLGFIP 350

BLAST of Spg022821 vs. ExPASy Swiss-Prot
Match: Q9LSB8 (Putative pentatricopeptide repeat-containing protein At3g15930 OS=Arabidopsis thaliana OX=3702 GN=PCMP-E51 PE=3 SV=2)

HSP 1 Score: 172.2 bits (435), Expect = 8.6e-42
Identity = 100/288 (34.72%), Postives = 157/288 (54.51%), Query Frame = 0

Query: 16  ERMFDYVQ----DPSLFVYNVMVKAYNKRGE-KIHVFVVKTGMDYDDYVCNSLMDMYAEL 75
           +R+ +YV     +PSL + N +V AY   GE  I V + ++    D     S++  Y E 
Sbjct: 256 KRVHEYVSECKTEPSLRLENALVNAYAACGEMDIAVRIFRSMKARDVISWTSIVKGYVER 315

Query: 76  SKIGNAKKLFDEMPTRDYVSWNVMIAGYVRCRRFEDALNTFKEMQQESNEKPSEAAVVST 135
             +  A+  FD+MP RD +SW +MI GY+R   F ++L  F+EM Q +   P E  +VS 
Sbjct: 316 GNLKLARTYFDQMPVRDRISWTIMIDGYLRAGCFNESLEIFREM-QSAGMIPDEFTMVSV 375

Query: 136 LSAFLHDYVRKELGFTTIINNALLDMCTKFGSSRAREMDSWITM---------DAVVGTA 195
           L+A                       C   GS    E+  WI           D VVG A
Sbjct: 376 LTA-----------------------CAHLGS---LEIGEWIKTYIDKNKIKNDVVVGNA 435

Query: 196 LIEMYSKCGCVDKSLEIFCELKEKDTTSWTSIICGLAMNGKTGETPRLFTAMELVRARLD 255
           LI+MY KCGC +K+ ++F ++ ++D  +WT+++ GLA NG+  E  ++F  M+ +  + D
Sbjct: 436 LIDMYFKCGCSEKAQKVFHDMDQRDKFTWTAMVVGLANNGQGQEAIKVFFQMQDMSIQPD 495

Query: 256 DITFIGVLSACSHGGLVEEGR----WMKKVYRVKPKVEHYGCVIDLLG 286
           DIT++GVLSAC+H G+V++ R     M+  +R++P + HYGC++D+LG
Sbjct: 496 DITYLGVLSACNHSGMVDQARKFFAKMRSDHRIEPSLVHYGCMVDMLG 516

BLAST of Spg022821 vs. ExPASy Swiss-Prot
Match: Q7Y211 (Pentatricopeptide repeat-containing protein At3g57430, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PCMP-H81 PE=2 SV=2)

HSP 1 Score: 171.0 bits (432), Expect = 1.9e-41
Identity = 96/251 (38.25%), Postives = 137/251 (54.58%), Query Frame = 0

Query: 40  RGEKIHVFVVKTGMDYDDYVCNSLMDMYAELSKIGNAKKLFDEMPTRDYVSWNVMIAGYV 99
           R E IH FVVK G+D D +V N+LMDMY+ L KI  A ++F +M  RD V+WN MI GYV
Sbjct: 423 RKEAIHGFVVKRGLDRDRFVQNTLMDMYSRLGKIDIAMRIFGKMEDRDLVTWNTMITGYV 482

Query: 100 RCRRFEDALNTFKEMQQESNEKPSEAAVVSTLSAFLHDYVRKELGFTTIINN-ALLDMCT 159
                EDAL    +MQ    +    A+ VS             +   TI+ + A L    
Sbjct: 483 FSEHHEDALLLLHKMQNLERKVSKGASRVSLKP--------NSITLMTILPSCAALSALA 542

Query: 160 KFGSSRAREMDSWITMDAVVGTALIEMYSKCGCVDKSLEIFCELKEKDTTSWTSIICGLA 219
           K     A  + + +  D  VG+AL++MY+KCGC+  S ++F ++ +K+  +W  II    
Sbjct: 543 KGKEIHAYAIKNNLATDVAVGSALVDMYAKCGCLQMSRKVFDQIPQKNVITWNVIIMAYG 602

Query: 220 MNGKTGETPRLFTAMELVRARLDDITFIGVLSACSHGGLVEEG----RWMKKVYRVKPKV 279
           M+G   E   L   M +   + +++TFI V +ACSH G+V+EG      MK  Y V+P  
Sbjct: 603 MHGNGQEAIDLLRMMMVQGVKPNEVTFISVFAACSHSGMVDEGLRIFYVMKPDYGVEPSS 662

Query: 280 EHYGCVIDLLG 286
           +HY CV+DLLG
Sbjct: 663 DHYACVVDLLG 665

BLAST of Spg022821 vs. ExPASy Swiss-Prot
Match: Q9SZT8 (Pentatricopeptide repeat-containing protein ELI1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ELI1 PE=3 SV=1)

HSP 1 Score: 165.6 bits (418), Expect = 8.1e-40
Identity = 104/308 (33.77%), Postives = 154/308 (50.00%), Query Frame = 0

Query: 24  DPSLFVYNVMVKAYN-KRGEKIHVFVVKTGMDYDDYVCNSLMDMYAELSKIGNAKKLFDE 83
           +P+ F ++ ++K+ + K G+ IH  V+K G+  D YV   L+D+YA+   + +A+K+FD 
Sbjct: 127 NPNEFTFSSLLKSCSTKSGKLIHTHVLKFGLGIDPYVATGLVDVYAKGGDVVSAQKVFDR 186

Query: 84  MP-------------------------------TRDYVSWNVMIAGYVRCRRFEDALNTF 143
           MP                                RD VSWNVMI GY +     DAL  F
Sbjct: 187 MPERSLVSSTAMITCYAKQGNVEAARALFDSMCERDIVSWNVMIDGYAQHGFPNDALMLF 246

Query: 144 KEMQQESNEKPSEAAVVSTLSAFLHDYVRKELGFTTIINNALLDMCTKFGSSRAREMDSW 203
           +++  E   KP E  VV+ LSA                       C++ G   A E   W
Sbjct: 247 QKLLAEGKPKPDEITVVAALSA-----------------------CSQIG---ALETGRW 306

Query: 204 ---------ITMDAVVGTALIEMYSKCGCVDKSLEIFCELKEKDTTSWTSIICGLAMNGK 263
                    I ++  V T LI+MYSKCG +++++ +F +   KD  +W ++I G AM+G 
Sbjct: 307 IHVFVKSSRIRLNVKVCTGLIDMYSKCGSLEEAVLVFNDTPRKDIVAWNAMIAGYAMHGY 366

Query: 264 TGETPRLFTAME-LVRARLDDITFIGVLSACSHGGLVEEG----RWMKKVYRVKPKVEHY 286
           + +  RLF  M+ +   +  DITFIG L AC+H GLV EG      M + Y +KPK+EHY
Sbjct: 367 SQDALRLFNEMQGITGLQPTDITFIGTLQACAHAGLVNEGIRIFESMGQEYGIKPKIEHY 408

BLAST of Spg022821 vs. ExPASy TrEMBL
Match: A0A6J1H7E9 (pentatricopeptide repeat-containing protein At1g31430 OS=Cucurbita moschata OX=3662 GN=LOC111461170 PE=4 SV=1)

HSP 1 Score: 402.9 bits (1034), Expect = 1.1e-108
Identity = 234/443 (52.82%), Postives = 253/443 (57.11%), Query Frame = 0

Query: 1   MAFCADPSLGNLRYAERMFDYVQDPSLFVYNVMVKAYNKR-------------------- 60
           MAFCADPS+GNLRY ERMFDY+Q PSLFVYNVMVKAY KR                    
Sbjct: 81  MAFCADPSVGNLRYTERMFDYIQGPSLFVYNVMVKAYAKRGIFRKVLLLFQRLREDELWP 140

Query: 61  --------------------GEKIHVFVVKTGMDYDDYVCNSLMDMYAELSKIGNAKKLF 120
                               GEK+H FVVKTGM  D YVCNSLMDMYAEL K+GNAKKLF
Sbjct: 141 DNFTYPFVLKAIGCLRDARKGEKVHGFVVKTGMVCDTYVCNSLMDMYAELGKVGNAKKLF 200

Query: 121 DEMPTRDYVSWNVMIAGYVRCRRFEDALNTFKEMQQESNEKPSEAAVVSTLSAF------ 180
           DEMP RD VSWNV+I+GYVRCRRFEDA+N FKEMQQESNEKP EA VVSTLSA       
Sbjct: 201 DEMPRRDSVSWNVLISGYVRCRRFEDAVNVFKEMQQESNEKPGEATVVSTLSACTALKNL 260

Query: 181 -----LHDYVRKELGFTTIINNALLDMCTK------------------------------ 240
                +H+YVRK+LGFTTIINNALLDM  K                              
Sbjct: 261 ELGVEIHNYVRKQLGFTTIINNALLDMYAKCGCLDIARKIFDEMPMRNVICWTSMISSYI 320

Query: 241 -----------FGSSRAREMDSW------------------------------------- 286
                      F  S  R++  W                                     
Sbjct: 321 NCGDLTEARDLFDRSPVRDVVLWTAMINGYVQFNHFDEAVALFREMQFQKVKPDKFTVVA 380

BLAST of Spg022821 vs. ExPASy TrEMBL
Match: A0A6J1CTE5 (pentatricopeptide repeat-containing protein At1g31430 OS=Momordica charantia OX=3673 GN=LOC111014415 PE=4 SV=1)

HSP 1 Score: 401.7 bits (1031), Expect = 2.5e-108
Identity = 234/443 (52.82%), Postives = 256/443 (57.79%), Query Frame = 0

Query: 1   MAFCADPSLGNLRYAERMFDYVQDPSLFVYNVMVKAYNKR-------------------- 60
           MAFCADPSLGNLRYAER+FDY+Q+P LFVYNVMVKAY KR                    
Sbjct: 74  MAFCADPSLGNLRYAERVFDYIQEPCLFVYNVMVKAYAKRGIFRKVILLFQRLKEDGLWP 133

Query: 61  --------------------GEKIHVFVVKTGMDYDDYVCNSLMDMYAELSKIGNAKKLF 120
                               GEKIH FVVKTGMD+D+YVCNSLMDMYAEL KI  A+KLF
Sbjct: 134 DNFTYPFVLKAIGCLRDLCHGEKIHGFVVKTGMDFDNYVCNSLMDMYAELGKIEYAEKLF 193

Query: 121 DEMPTRDYVSWNVMIAGYVRCRRFEDALNTFKEMQQESNEKPSEAAVVSTLSAF------ 180
           DEM T+D VSWNV+I+GYVRCRRFEDA+NTFKEMQ+ESN KP EA +VSTLSA       
Sbjct: 194 DEMHTKDSVSWNVLISGYVRCRRFEDAINTFKEMQRESNVKPGEAGIVSTLSACTALKNL 253

Query: 181 -----LHDYVRKELGFTTIINNALLDMCTK------------------------------ 240
                +H+YVR ELGFTTIINNALLDM  K                              
Sbjct: 254 ELGEEIHNYVRNELGFTTIINNALLDMYAKCGRLNIARKVFDEMPMKNVICWTSMISGYI 313

Query: 241 -----------FGSSRAREMDSW------------------------------------- 286
                      F  S  R++  W                                     
Sbjct: 314 NCGDLKEARDLFDISPVRDVVLWTAMINGYVQFNHFDEAVALFRKMQIQKVKPDKFTVVA 373

BLAST of Spg022821 vs. ExPASy TrEMBL
Match: A0A6J1KW17 (pentatricopeptide repeat-containing protein At1g31430 OS=Cucurbita maxima OX=3661 GN=LOC111498101 PE=4 SV=1)

HSP 1 Score: 399.4 bits (1025), Expect = 1.2e-107
Identity = 232/443 (52.37%), Postives = 251/443 (56.66%), Query Frame = 0

Query: 1   MAFCADPSLGNLRYAERMFDYVQDPSLFVYNVMVKAYNKR-------------------- 60
           MAFCADPS+GNL Y ERMFDY+Q PSLFVYNVMVKAY KR                    
Sbjct: 81  MAFCADPSVGNLHYTERMFDYIQGPSLFVYNVMVKAYAKRGIFRKVLLLFQRLREDELWP 140

Query: 61  --------------------GEKIHVFVVKTGMDYDDYVCNSLMDMYAELSKIGNAKKLF 120
                               GEK+H FVVKTGM  D YVCNSLMDMYAEL K+GNAKKLF
Sbjct: 141 DNFTYPFVLKAIGCLRDARKGEKVHGFVVKTGMVCDTYVCNSLMDMYAELGKVGNAKKLF 200

Query: 121 DEMPTRDYVSWNVMIAGYVRCRRFEDALNTFKEMQQESNEKPSEAAVVSTLSAF------ 180
           DEMP RD VSWNV+I+GYVRCRRFEDA+N FKEMQQESNEKP EA VVSTLSA       
Sbjct: 201 DEMPRRDSVSWNVLISGYVRCRRFEDAVNVFKEMQQESNEKPGEATVVSTLSACTALKNL 260

Query: 181 -----LHDYVRKELGFTTIINNALLDMCTK------------------------------ 240
                +H+YVRKELGFTTIINNALLDM  K                              
Sbjct: 261 ALGVEIHNYVRKELGFTTIINNALLDMYAKCGCLDIARKIFDEMPMRNVICWTSMISSYI 320

Query: 241 -----------FGSSRAREMDSW------------------------------------- 286
                      F  S  R++  W                                     
Sbjct: 321 NCGDLTEARDLFDRSPVRDVVLWTAMINGYVQFNHFDEAVALFREMQFQKVKPDKFTVVA 380

BLAST of Spg022821 vs. ExPASy TrEMBL
Match: A0A1S3CPD2 (pentatricopeptide repeat-containing protein At1g31430 OS=Cucumis melo OX=3656 GN=LOC103502722 PE=4 SV=1)

HSP 1 Score: 387.9 bits (995), Expect = 3.7e-104
Identity = 227/443 (51.24%), Postives = 250/443 (56.43%), Query Frame = 0

Query: 1   MAFCADPSLGNLRYAERMFDYVQDPSLFVYNVMVKAYNKR-------------------- 60
           MAFCAD SLGNLRYAE++FDYVQDPSLFVYNVMVK Y KR                    
Sbjct: 74  MAFCADLSLGNLRYAEKIFDYVQDPSLFVYNVMVKMYAKRGLLRKVLLLFQQLREDQLWP 133

Query: 61  --------------------GEKIHVFVVKTGMDYDDYVCNSLMDMYAELSKIGNAKKLF 120
                               GEK+H FVVKTGM+ D+YVCNSLMDMY+EL  + NAKKLF
Sbjct: 134 DNFTYPFVLKAIGCLRDVGQGEKLHGFVVKTGMNLDNYVCNSLMDMYSELGNVENAKKLF 193

Query: 121 DEMPTRDYVSWNVMIAGYVRCRRFEDALNTFKEMQQESNEKPSEAAVVSTLSAF------ 180
           DEM TRD VSWNVMI+GYV CRRFEDA+NTF+EMQQE NEKP EA VVSTLSA       
Sbjct: 194 DEMTTRDSVSWNVMISGYVGCRRFEDAINTFREMQQEGNEKPDEATVVSTLSACTALKNL 253

Query: 181 -----LHDYVRKELGFTTIINNALLDMCTK------------------------------ 240
                +H+YVRKELGFT  I+NALLDM  K                              
Sbjct: 254 ELGDEIHNYVRKELGFTPRIDNALLDMYAKCGCLNISRNIFDEMPMKNVICWTSMISGYI 313

Query: 241 -----------FGSSRAREMDSW------------------------------------- 286
                      F  S  R++  W                                     
Sbjct: 314 NCGDLREARDLFDKSPVRDVVLWTAMINGYVQFHHFDDAVALFREMQIQKVKPDKFTVVT 373

BLAST of Spg022821 vs. ExPASy TrEMBL
Match: A0A5A7TXP7 (Pentatricopeptide repeat-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold216G00060 PE=4 SV=1)

HSP 1 Score: 387.9 bits (995), Expect = 3.7e-104
Identity = 227/443 (51.24%), Postives = 250/443 (56.43%), Query Frame = 0

Query: 1   MAFCADPSLGNLRYAERMFDYVQDPSLFVYNVMVKAYNKR-------------------- 60
           MAFCAD SLGNLRYAE++FDYVQDPSLFVYNVMVK Y KR                    
Sbjct: 131 MAFCADLSLGNLRYAEKIFDYVQDPSLFVYNVMVKMYAKRGLLRKVLLLFQQLREDQLWP 190

Query: 61  --------------------GEKIHVFVVKTGMDYDDYVCNSLMDMYAELSKIGNAKKLF 120
                               GEK+H FVVKTGM+ D+YVCNSLMDMY+EL  + NAKKLF
Sbjct: 191 DNFTYPFVLKAIGCLRDVGQGEKLHGFVVKTGMNLDNYVCNSLMDMYSELGNVENAKKLF 250

Query: 121 DEMPTRDYVSWNVMIAGYVRCRRFEDALNTFKEMQQESNEKPSEAAVVSTLSAF------ 180
           DEM TRD VSWNVMI+GYV CRRFEDA+NTF+EMQQE NEKP EA VVSTLSA       
Sbjct: 251 DEMTTRDSVSWNVMISGYVGCRRFEDAINTFREMQQEGNEKPDEATVVSTLSACTALKNL 310

Query: 181 -----LHDYVRKELGFTTIINNALLDMCTK------------------------------ 240
                +H+YVRKELGFT  I+NALLDM  K                              
Sbjct: 311 ELGDEIHNYVRKELGFTPRIDNALLDMYAKCGCLNISRNIFDEMPMKNVICWTSMISGYI 370

Query: 241 -----------FGSSRAREMDSW------------------------------------- 286
                      F  S  R++  W                                     
Sbjct: 371 NCGDLREARDLFDKSPVRDVVLWTAMINGYVQFHHFDDAVALFREMQIQKVKPDKFTVVT 430

BLAST of Spg022821 vs. TAIR 10
Match: AT1G31430.1 (Pentatricopeptide repeat (PPR-like) superfamily protein )

HSP 1 Score: 218.0 bits (554), Expect = 9.7e-57
Identity = 138/383 (36.03%), Postives = 180/383 (47.00%), Query Frame = 0

Query: 25  PSLFVYNVMVKAYNK-----RGEKIHVFVVKTGMDYDDYVCNSLMDMYAELSKIGNAKKL 84
           P  F   V++K+  +      GEK+H + VK G+++D YV NSLM MYA L KI    K+
Sbjct: 44  PDNFTLPVVLKSIGRLRKVIEGEKVHGYAVKAGLEFDSYVSNSLMGMYASLGKIEITHKV 103

Query: 85  FDEMPTRDYVSWNVMIAGYVRCRRFEDALNTFKEMQQESNEKPSEAAVVSTLSAF----- 144
           FDEMP RD VSWN +I+ YV   RFEDA+  FK M QESN K  E  +VSTLSA      
Sbjct: 104 FDEMPQRDVVSWNGLISSYVGNGRFEDAIGVFKRMSQESNLKFDEGTIVSTLSACSALKN 163

Query: 145 ------LHDYVRKELGFTTIINNALLDMCTK----------FGSSRAREMDSW------- 204
                 ++ +V  E   +  I NAL+DM  K          F S R + +  W       
Sbjct: 164 LEIGERIYRFVVTEFEMSVRIGNALVDMFCKCGCLDKARAVFDSMRDKNVKCWTSMVFGY 223

Query: 205 ------------------------------------------------------------ 264
                                                                       
Sbjct: 224 VSTGRIDEARVLFERSPVKDVVLWTAMMNGYVQFNRFDEALELFRCMQTAGIRPDNFVLV 283

Query: 265 --------------------------ITMDAVVGTALIEMYSKCGCVDKSLEIFCELKEK 285
                                     +T+D VVGTAL++MY+KCGC++ +LE+F E+KE+
Sbjct: 284 SLLTGCAQTGALEQGKWIHGYINENRVTVDKVVGTALVDMYAKCGCIETALEVFYEIKER 343

BLAST of Spg022821 vs. TAIR 10
Match: AT5G06540.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 174.9 bits (442), Expect = 9.5e-44
Identity = 109/349 (31.23%), Postives = 176/349 (50.43%), Query Frame = 0

Query: 1   MAFCADPSLGN-----LRYAERMFDYVQDPSLFVYNVMVKAYNKR--------------- 60
           +A C D S  N     L YA  +F  +Q+P+LFV+N++++ ++                 
Sbjct: 51  LALCVDDSTFNKPTNLLGYAYGIFSQIQNPNLFVFNLLIRCFSTGAEPSKAFGFYTQMLK 110

Query: 61  -------------------------GEKIHVFVVKTGMDYDDYVCNSLMDMYAELSKIGN 120
                                    GE+ H  +V+ G   D YV NSL+ MYA    I  
Sbjct: 111 SRIWPDNITFPFLIKASSEMECVLVGEQTHSQIVRFGFQNDVYVENSLVHMYANCGFIAA 170

Query: 121 AKKLFDEMPTRDYVSWNVMIAGYVRCRRFEDALNTFKEMQQES--------NEKPSEAAV 180
           A ++F +M  RD VSW  M+AGY +C   E+A   F EM   +        N        
Sbjct: 171 AGRIFGQMGFRDVVSWTSMVAGYCKCGMVENAREMFDEMPHRNLFTWSIMINGYAKNNCF 230

Query: 181 VSTLSAFLHDYVRKE-LGFTTIINNALLDMCTKFGS----SRARE--MDSWITMDAVVGT 240
              +  F  +++++E +     +  +++  C   G+     RA E  + S +T++ ++GT
Sbjct: 231 EKAIDLF--EFMKREGVVANETVMVSVISSCAHLGALEFGERAYEYVVKSHMTVNLILGT 290

Query: 241 ALIEMYSKCGCVDKSLEIFCELKEKDTTSWTSIICGLAMNGKTGETPRLFTAMELVRARL 286
           AL++M+ +CG ++K++ +F  L E D+ SW+SII GLA++G   +    F+ M  +    
Sbjct: 291 ALVDMFWRCGDIEKAIHVFEGLPETDSLSWSSIIKGLAVHGHAHKAMHYFSQMISLGFIP 350

BLAST of Spg022821 vs. TAIR 10
Match: AT3G15930.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 172.2 bits (435), Expect = 6.1e-43
Identity = 100/288 (34.72%), Postives = 157/288 (54.51%), Query Frame = 0

Query: 16  ERMFDYVQ----DPSLFVYNVMVKAYNKRGE-KIHVFVVKTGMDYDDYVCNSLMDMYAEL 75
           +R+ +YV     +PSL + N +V AY   GE  I V + ++    D     S++  Y E 
Sbjct: 256 KRVHEYVSECKTEPSLRLENALVNAYAACGEMDIAVRIFRSMKARDVISWTSIVKGYVER 315

Query: 76  SKIGNAKKLFDEMPTRDYVSWNVMIAGYVRCRRFEDALNTFKEMQQESNEKPSEAAVVST 135
             +  A+  FD+MP RD +SW +MI GY+R   F ++L  F+EM Q +   P E  +VS 
Sbjct: 316 GNLKLARTYFDQMPVRDRISWTIMIDGYLRAGCFNESLEIFREM-QSAGMIPDEFTMVSV 375

Query: 136 LSAFLHDYVRKELGFTTIINNALLDMCTKFGSSRAREMDSWITM---------DAVVGTA 195
           L+A                       C   GS    E+  WI           D VVG A
Sbjct: 376 LTA-----------------------CAHLGS---LEIGEWIKTYIDKNKIKNDVVVGNA 435

Query: 196 LIEMYSKCGCVDKSLEIFCELKEKDTTSWTSIICGLAMNGKTGETPRLFTAMELVRARLD 255
           LI+MY KCGC +K+ ++F ++ ++D  +WT+++ GLA NG+  E  ++F  M+ +  + D
Sbjct: 436 LIDMYFKCGCSEKAQKVFHDMDQRDKFTWTAMVVGLANNGQGQEAIKVFFQMQDMSIQPD 495

Query: 256 DITFIGVLSACSHGGLVEEGR----WMKKVYRVKPKVEHYGCVIDLLG 286
           DIT++GVLSAC+H G+V++ R     M+  +R++P + HYGC++D+LG
Sbjct: 496 DITYLGVLSACNHSGMVDQARKFFAKMRSDHRIEPSLVHYGCMVDMLG 516

BLAST of Spg022821 vs. TAIR 10
Match: AT3G57430.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 171.0 bits (432), Expect = 1.4e-42
Identity = 96/251 (38.25%), Postives = 137/251 (54.58%), Query Frame = 0

Query: 40  RGEKIHVFVVKTGMDYDDYVCNSLMDMYAELSKIGNAKKLFDEMPTRDYVSWNVMIAGYV 99
           R E IH FVVK G+D D +V N+LMDMY+ L KI  A ++F +M  RD V+WN MI GYV
Sbjct: 423 RKEAIHGFVVKRGLDRDRFVQNTLMDMYSRLGKIDIAMRIFGKMEDRDLVTWNTMITGYV 482

Query: 100 RCRRFEDALNTFKEMQQESNEKPSEAAVVSTLSAFLHDYVRKELGFTTIINN-ALLDMCT 159
                EDAL    +MQ    +    A+ VS             +   TI+ + A L    
Sbjct: 483 FSEHHEDALLLLHKMQNLERKVSKGASRVSLKP--------NSITLMTILPSCAALSALA 542

Query: 160 KFGSSRAREMDSWITMDAVVGTALIEMYSKCGCVDKSLEIFCELKEKDTTSWTSIICGLA 219
           K     A  + + +  D  VG+AL++MY+KCGC+  S ++F ++ +K+  +W  II    
Sbjct: 543 KGKEIHAYAIKNNLATDVAVGSALVDMYAKCGCLQMSRKVFDQIPQKNVITWNVIIMAYG 602

Query: 220 MNGKTGETPRLFTAMELVRARLDDITFIGVLSACSHGGLVEEG----RWMKKVYRVKPKV 279
           M+G   E   L   M +   + +++TFI V +ACSH G+V+EG      MK  Y V+P  
Sbjct: 603 MHGNGQEAIDLLRMMMVQGVKPNEVTFISVFAACSHSGMVDEGLRIFYVMKPDYGVEPSS 662

Query: 280 EHYGCVIDLLG 286
           +HY CV+DLLG
Sbjct: 663 DHYACVVDLLG 665

BLAST of Spg022821 vs. TAIR 10
Match: AT4G37380.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 165.6 bits (418), Expect = 5.7e-41
Identity = 104/308 (33.77%), Postives = 154/308 (50.00%), Query Frame = 0

Query: 24  DPSLFVYNVMVKAYN-KRGEKIHVFVVKTGMDYDDYVCNSLMDMYAELSKIGNAKKLFDE 83
           +P+ F ++ ++K+ + K G+ IH  V+K G+  D YV   L+D+YA+   + +A+K+FD 
Sbjct: 127 NPNEFTFSSLLKSCSTKSGKLIHTHVLKFGLGIDPYVATGLVDVYAKGGDVVSAQKVFDR 186

Query: 84  MP-------------------------------TRDYVSWNVMIAGYVRCRRFEDALNTF 143
           MP                                RD VSWNVMI GY +     DAL  F
Sbjct: 187 MPERSLVSSTAMITCYAKQGNVEAARALFDSMCERDIVSWNVMIDGYAQHGFPNDALMLF 246

Query: 144 KEMQQESNEKPSEAAVVSTLSAFLHDYVRKELGFTTIINNALLDMCTKFGSSRAREMDSW 203
           +++  E   KP E  VV+ LSA                       C++ G   A E   W
Sbjct: 247 QKLLAEGKPKPDEITVVAALSA-----------------------CSQIG---ALETGRW 306

Query: 204 ---------ITMDAVVGTALIEMYSKCGCVDKSLEIFCELKEKDTTSWTSIICGLAMNGK 263
                    I ++  V T LI+MYSKCG +++++ +F +   KD  +W ++I G AM+G 
Sbjct: 307 IHVFVKSSRIRLNVKVCTGLIDMYSKCGSLEEAVLVFNDTPRKDIVAWNAMIAGYAMHGY 366

Query: 264 TGETPRLFTAME-LVRARLDDITFIGVLSACSHGGLVEEG----RWMKKVYRVKPKVEHY 286
           + +  RLF  M+ +   +  DITFIG L AC+H GLV EG      M + Y +KPK+EHY
Sbjct: 367 SQDALRLFNEMQGITGLQPTDITFIGTLQACAHAGLVNEGIRIFESMGQEYGIKPKIEHY 408