Spg021929 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg021929 vs. NCBI nr
Match: XP_023530984.1 (uncharacterized protein LOC111793373 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 912.1 bits (2356), Expect = 2.1e-261
Identity = 471/522 (90.23%), Postives = 498/522 (95.40%), Query Frame = 0

Query: 1   MEAPSLSNRWIATVASIWIQCICGPSYTFGIYSSALKSTQNYHQSTLDTVSVFKDIGATA 60
           MEAP  SNRWIATVASIWIQCICGPSYTFGIYSSALKS+QNY QSTLDTVSVFKDIGA+A
Sbjct: 1   MEAPP-SNRWIATVASIWIQCICGPSYTFGIYSSALKSSQNYDQSTLDTVSVFKDIGASA 60

Query: 61  GVLAGLLYSAVVSTDRPRRPWIVLTVGAIQCFLGYIFMWAAVSGLIPRPPVPAMCLFMFL 120
           GVLAGLLYSAVVSTDRPRRPWIVL+VGAIQCFLGYIF+WAAVSGLIPRPPV AMCLFMFL
Sbjct: 61  GVLAGLLYSAVVSTDRPRRPWIVLSVGAIQCFLGYIFIWAAVSGLIPRPPVSAMCLFMFL 120

Query: 121 AVHAQVYFNTANVVTGVHNFQLYGGTIVGILKGFLGLSGAVLIQFSNTFYNGNPVNYLLM 180
           AVHAQV+FNTANVVTGVHNFQLYGGTI+GILKGFLGLSGAVLIQFSNTFYNG+P+NYLLM
Sbjct: 121 AVHAQVFFNTANVVTGVHNFQLYGGTIIGILKGFLGLSGAVLIQFSNTFYNGDPINYLLM 180

Query: 181 LAILPALTTLLLMRFVVIDKTETGNESTHLNSLSSIALIISGYLTILIVLDNVLTLPTWA 240
           LAILPALTT+LLMRFVVI KTE GNESTHLNSLS IALIIS YLTILI+LDNVL LPTW 
Sbjct: 181 LAILPALTTVLLMRFVVIQKTEIGNESTHLNSLSKIALIISCYLTILIILDNVLILPTWV 240

Query: 241 RLFTFILLLALLASPLGIAVRAQTEDSVFKTKLQNTDDSVEYHRIPSEDQRNEQLTIVTD 300
           RLFTF++LL LLASPLGIA RAQTEDS  KTKLQ+TD+SVEYH+IP E+QRN+QL +VT+
Sbjct: 241 RLFTFVILLILLASPLGIANRAQTEDSASKTKLQSTDNSVEYHQIPGEEQRNDQLMMVTN 300

Query: 301 GEMNVIEAIGTVNFWILFFAMMCGMGSGLATINNMNQLGQSLGYGTVEINTFVSLWSIWN 360
           GEMNVIEAIGTVNFW+LF AMMCGMGSGLATINNMNQLGQSLGY TVEI+TFVSLWSIWN
Sbjct: 301 GEMNVIEAIGTVNFWLLFSAMMCGMGSGLATINNMNQLGQSLGYKTVEIHTFVSLWSIWN 360

Query: 361 FLGRLGSGYASDLLLWKLGWARPLLMAIALLTMSVGHIIIASGFSGNLYLGSVIVGICYG 420
           FLGRLGSGYASDLLL +LGWARPLLMA+ALLTMSVGHIIIASG S NLYLGSVIVGICYG
Sbjct: 361 FLGRLGSGYASDLLLRELGWARPLLMAVALLTMSVGHIIIASGSSWNLYLGSVIVGICYG 420

Query: 421 AQWSLMPAIPAEIFGIRHMGTIYNTITVASPIGSYVLSVRVIGYIYDREASAGDNSCSGK 480
           +QWSLMPAI AEIFGIRHMGTIYNTITVASPIGSYVLSVRVIGYIYDREASAGDNSCSGK
Sbjct: 421 SQWSLMPAITAEIFGIRHMGTIYNTITVASPIGSYVLSVRVIGYIYDREASAGDNSCSGK 480

Query: 481 HCFITSFLIMAAVAFLGFVVAVALFFRTRRFYQVLVQRRLKD 523
           HCF+TSFLIMAAVAFLGFVVA ALF RTRRFYQ+LVQR+LKD
Sbjct: 481 HCFMTSFLIMAAVAFLGFVVAFALFLRTRRFYQLLVQRQLKD 521

BLAST of Spg021929 vs. NCBI nr
Match: KAG6587515.1 (Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 910.6 bits (2352), Expect = 6.2e-261
Identity = 470/522 (90.04%), Postives = 498/522 (95.40%), Query Frame = 0

Query: 1   MEAPSLSNRWIATVASIWIQCICGPSYTFGIYSSALKSTQNYHQSTLDTVSVFKDIGATA 60
           MEAP  SNRWIATVASIWIQCICGPSYTFGIYSSALKS+QNY QSTLDTVSVFKDIGATA
Sbjct: 1   MEAPP-SNRWIATVASIWIQCICGPSYTFGIYSSALKSSQNYDQSTLDTVSVFKDIGATA 60

Query: 61  GVLAGLLYSAVVSTDRPRRPWIVLTVGAIQCFLGYIFMWAAVSGLIPRPPVPAMCLFMFL 120
           GVLAGLLYSAVVSTDRP RPWIVL+VGAIQCFLGYIF+WAAVSGLIPRP V  MCLFMFL
Sbjct: 61  GVLAGLLYSAVVSTDRPHRPWIVLSVGAIQCFLGYIFIWAAVSGLIPRPSVSTMCLFMFL 120

Query: 121 AVHAQVYFNTANVVTGVHNFQLYGGTIVGILKGFLGLSGAVLIQFSNTFYNGNPVNYLLM 180
           AVHAQV+FNTANVVTGVHNFQLYGGTI+GILKGFLGLSGAVLIQFSNTFY+G+P++YLLM
Sbjct: 121 AVHAQVFFNTANVVTGVHNFQLYGGTIIGILKGFLGLSGAVLIQFSNTFYSGDPISYLLM 180

Query: 181 LAILPALTTLLLMRFVVIDKTETGNESTHLNSLSSIALIISGYLTILIVLDNVLTLPTWA 240
           LAILPALTT+LLMRFVVI+KTE GNESTHLNSLS IALIIS YLTILI+LDNV  LPTW 
Sbjct: 181 LAILPALTTVLLMRFVVIEKTEIGNESTHLNSLSKIALIISCYLTILIILDNVFILPTWV 240

Query: 241 RLFTFILLLALLASPLGIAVRAQTEDSVFKTKLQNTDDSVEYHRIPSEDQRNEQLTIVTD 300
           RLFTF+LLL LLASPLGIA RAQTEDSV KTKLQ+TD+SVEYH+IP E+QRN+QL +VT+
Sbjct: 241 RLFTFVLLLILLASPLGIANRAQTEDSVSKTKLQSTDNSVEYHQIPGEEQRNDQLMMVTN 300

Query: 301 GEMNVIEAIGTVNFWILFFAMMCGMGSGLATINNMNQLGQSLGYGTVEINTFVSLWSIWN 360
           GEMNVIEAIGTVNFW+LF AMMCGMGSGLATINNMNQLGQSLGY  VEI+TFVSLWSIWN
Sbjct: 301 GEMNVIEAIGTVNFWLLFSAMMCGMGSGLATINNMNQLGQSLGYKPVEIHTFVSLWSIWN 360

Query: 361 FLGRLGSGYASDLLLWKLGWARPLLMAIALLTMSVGHIIIASGFSGNLYLGSVIVGICYG 420
           FLGRLGSGYASDLLL +LGWARPLLMA+ALLTMSVGHIIIASGFSGNLYLGSVIVGICYG
Sbjct: 361 FLGRLGSGYASDLLLRELGWARPLLMAVALLTMSVGHIIIASGFSGNLYLGSVIVGICYG 420

Query: 421 AQWSLMPAIPAEIFGIRHMGTIYNTITVASPIGSYVLSVRVIGYIYDREASAGDNSCSGK 480
           +QWSLMPAI AEIFGIRHMGTIYNTITVASPIGSYVLSVRVIGYIYDREASAGDNSCSGK
Sbjct: 421 SQWSLMPAITAEIFGIRHMGTIYNTITVASPIGSYVLSVRVIGYIYDREASAGDNSCSGK 480

Query: 481 HCFITSFLIMAAVAFLGFVVAVALFFRTRRFYQVLVQRRLKD 523
           HCF+TSFLIMAAVAFLGFVVAVALF RTRRFYQ+LVQR+LKD
Sbjct: 481 HCFMTSFLIMAAVAFLGFVVAVALFLRTRRFYQLLVQRQLKD 521

BLAST of Spg021929 vs. NCBI nr
Match: KAG7021495.1 (Protein NUCLEAR FUSION DEFECTIVE 4 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 897.5 bits (2318), Expect = 5.4e-257
Identity = 462/515 (89.71%), Postives = 491/515 (95.34%), Query Frame = 0

Query: 7    SNRWIATVASIWIQCICGPSYTFGIYSSALKSTQNYHQSTLDTVSVFKDIGATAGVLAGL 66
            SNRWIATVASIWIQCICGPSYTFGIYSSALKS+QNY QSTLDTVSVFKDIGATAGVLAGL
Sbjct: 546  SNRWIATVASIWIQCICGPSYTFGIYSSALKSSQNYDQSTLDTVSVFKDIGATAGVLAGL 605

Query: 67   LYSAVVSTDRPRRPWIVLTVGAIQCFLGYIFMWAAVSGLIPRPPVPAMCLFMFLAVHAQV 126
            LYSAVVSTDRP RPWIVL+VGAIQCFLGYIF+WAAVSGLIPRP V  MCLFMFLAVHAQV
Sbjct: 606  LYSAVVSTDRPHRPWIVLSVGAIQCFLGYIFIWAAVSGLIPRPSVSTMCLFMFLAVHAQV 665

Query: 127  YFNTANVVTGVHNFQLYGGTIVGILKGFLGLSGAVLIQFSNTFYNGNPVNYLLMLAILPA 186
            +FNTANVVTGVHNFQLYGGTI+GILKGFLGLSGAVLIQFSNTFY+G+P++YLLMLAILPA
Sbjct: 666  FFNTANVVTGVHNFQLYGGTIIGILKGFLGLSGAVLIQFSNTFYSGDPISYLLMLAILPA 725

Query: 187  LTTLLLMRFVVIDKTETGNESTHLNSLSSIALIISGYLTILIVLDNVLTLPTWARLFTFI 246
            LTT+LLMRFVVI+KTE GNESTHLNSLS IALIIS YLTILI+LDNV  L TW RLFTF+
Sbjct: 726  LTTVLLMRFVVIEKTEIGNESTHLNSLSKIALIISCYLTILIILDNVFILTTWVRLFTFV 785

Query: 247  LLLALLASPLGIAVRAQTEDSVFKTKLQNTDDSVEYHRIPSEDQRNEQLTIVTDGEMNVI 306
            LLL LLASPLGIA RAQTEDSV KTKLQ+TD+SVEYH+IP E+QRN+QL +VT+GEMNVI
Sbjct: 786  LLLILLASPLGIANRAQTEDSVSKTKLQSTDNSVEYHQIPGEEQRNDQLMMVTNGEMNVI 845

Query: 307  EAIGTVNFWILFFAMMCGMGSGLATINNMNQLGQSLGYGTVEINTFVSLWSIWNFLGRLG 366
            EAIGTVNFW+LF AMMCGMGSGLATINNMNQLGQSLGY TVEI+TFVSLWSIWNFLGRLG
Sbjct: 846  EAIGTVNFWLLFSAMMCGMGSGLATINNMNQLGQSLGYKTVEIHTFVSLWSIWNFLGRLG 905

Query: 367  SGYASDLLLWKLGWARPLLMAIALLTMSVGHIIIASGFSGNLYLGSVIVGICYGAQWSLM 426
            SGYASDLLL +LGWARPLLMA+ALLTMSVGHIIIASGFSGNLYLGSVIVGICYG+QWSLM
Sbjct: 906  SGYASDLLLRELGWARPLLMAVALLTMSVGHIIIASGFSGNLYLGSVIVGICYGSQWSLM 965

Query: 427  PAIPAEIFGIRHMGTIYNTITVASPIGSYVLSVRVIGYIYDREASAGDNSCSGKHCFITS 486
            PAI AEIFGIRHMGTIYNTITVASPIGSYVLSVRVIGYIYDREASAGDNSCSGKHCF+TS
Sbjct: 966  PAITAEIFGIRHMGTIYNTITVASPIGSYVLSVRVIGYIYDREASAGDNSCSGKHCFMTS 1025

Query: 487  FLIMAAVAFLGFVVAVALFFRTRRFYQVLVQRRLK 522
            FLIMAAVAFLGFVVAVALF RTRRFYQ+L +R+ K
Sbjct: 1026 FLIMAAVAFLGFVVAVALFLRTRRFYQLLGKRKRK 1060

BLAST of Spg021929 vs. NCBI nr
Match: XP_022933362.1 (uncharacterized protein LOC111440723 [Cucurbita moschata])

HSP 1 Score: 882.9 bits (2280), Expect = 1.4e-252
Identity = 454/501 (90.62%), Postives = 483/501 (96.41%), Query Frame = 0

Query: 22  ICGPSYTFGIYSSALKSTQNYHQSTLDTVSVFKDIGATAGVLAGLLYSAVVSTDRPRRPW 81
           ICGPSYTFGIYSSALKS+QNY QSTLDTVSVFKDIGATAGVLAGLLYSAVVSTDRPRRPW
Sbjct: 13  ICGPSYTFGIYSSALKSSQNYDQSTLDTVSVFKDIGATAGVLAGLLYSAVVSTDRPRRPW 72

Query: 82  IVLTVGAIQCFLGYIFMWAAVSGLIPRPPVPAMCLFMFLAVHAQVYFNTANVVTGVHNFQ 141
           IVL+VGAIQCFLGYIF+WAAVSGLIPRPPV AMCLFMFLAVHAQV+FNTANVVTGVHNFQ
Sbjct: 73  IVLSVGAIQCFLGYIFIWAAVSGLIPRPPVSAMCLFMFLAVHAQVFFNTANVVTGVHNFQ 132

Query: 142 LYGGTIVGILKGFLGLSGAVLIQFSNTFYNGNPVNYLLMLAILPALTTLLLMRFVVIDKT 201
           LYGGTI+GILKGFLGLSGAVLIQFSNTFY+G+P++YLLMLAILPALTT+LLMRFVVI+KT
Sbjct: 133 LYGGTIIGILKGFLGLSGAVLIQFSNTFYSGDPISYLLMLAILPALTTVLLMRFVVIEKT 192

Query: 202 ETGNESTHLNSLSSIALIISGYLTILIVLDNVLTLPTWARLFTFILLLALLASPLGIAVR 261
           E GNESTHLNSLS IALIIS YLTILI+LDNV  LPTW RLFTF+LLL LLASPLGIA R
Sbjct: 193 EIGNESTHLNSLSKIALIISCYLTILIILDNVFFLPTWVRLFTFVLLLILLASPLGIANR 252

Query: 262 AQTEDSVFKTKLQNTDDSVEYHRIPSEDQRNEQLTIVTDGEMNVIEAIGTVNFWILFFAM 321
           AQTEDSV KTKLQ+TD+SVEYH+IP E+QRN+QL +VT+GEMNVIEAIGTVNFW+LF AM
Sbjct: 253 AQTEDSVSKTKLQSTDNSVEYHQIPGEEQRNDQLMMVTNGEMNVIEAIGTVNFWLLFSAM 312

Query: 322 MCGMGSGLATINNMNQLGQSLGYGTVEINTFVSLWSIWNFLGRLGSGYASDLLLWKLGWA 381
           MCGMGSGLATINNMNQLGQSLGY TVEI+TFVSLWSIWNFLGRLGSGYASDLLL +LGWA
Sbjct: 313 MCGMGSGLATINNMNQLGQSLGYKTVEIHTFVSLWSIWNFLGRLGSGYASDLLLRELGWA 372

Query: 382 RPLLMAIALLTMSVGHIIIASGFSGNLYLGSVIVGICYGAQWSLMPAIPAEIFGIRHMGT 441
           RPLLMA+ALLTMSVGHIIIASGFSGNLYLGSVIVGICYG+QWSLMPAI AEIFGIRHMGT
Sbjct: 373 RPLLMAVALLTMSVGHIIIASGFSGNLYLGSVIVGICYGSQWSLMPAITAEIFGIRHMGT 432

Query: 442 IYNTITVASPIGSYVLSVRVIGYIYDREASAGDNSCSGKHCFITSFLIMAAVAFLGFVVA 501
           IYNTITVASPIGSYVLSVRVIGYIYDREASAGDNSCSGKHCF+TSFLIMAAVAFLGF+VA
Sbjct: 433 IYNTITVASPIGSYVLSVRVIGYIYDREASAGDNSCSGKHCFMTSFLIMAAVAFLGFIVA 492

Query: 502 VALFFRTRRFYQVLVQRRLKD 523
           VALF RTRRFYQ+LVQR+LKD
Sbjct: 493 VALFLRTRRFYQLLVQRQLKD 513

BLAST of Spg021929 vs. NCBI nr
Match: XP_022134701.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC111006908 [Momordica charantia])

HSP 1 Score: 847.4 bits (2188), Expect = 6.4e-242
Identity = 440/524 (83.97%), Postives = 474/524 (90.46%), Query Frame = 0

Query: 1   MEAPSLSNRWIATVASIWIQCICGPSYTFGIYSSALKSTQNYHQSTLDTVSVFKDIGATA 60
           ME  SLSNRWIATVASIWIQCICGPSYTFGIYSSALKSTQ Y QSTLDTVSVF D+GATA
Sbjct: 1   MEVHSLSNRWIATVASIWIQCICGPSYTFGIYSSALKSTQAYDQSTLDTVSVFXDVGATA 60

Query: 61  GVLAGLLYSAVVSTDRPRRPWIVLTVGAIQCFLGYIFMWAAVSGLIPRPPVPAMCLFMFL 120
           GVLA LLYSAV S +RPRR WI+ TVGAIQCF GYIF+WAAVSGLIPRPPVPAMC FMFL
Sbjct: 61  GVLAVLLYSAVASNNRPRRSWIIHTVGAIQCFAGYIFIWAAVSGLIPRPPVPAMCFFMFL 120

Query: 121 AVHAQVYFNTANVVTGVHNFQLYGGTIVGILKGFLGLSGAVLIQFSNTFYNGNPVNYLLM 180
           AVHAQV+FNTANVVTGVHNFQLY  TIVGILKGF GL GAVLIQFSNTF N +P N L++
Sbjct: 121 AVHAQVFFNTANVVTGVHNFQLYSATIVGILKGFAGLXGAVLIQFSNTFCNRDPANNLVL 180

Query: 181 LAILPALTTLLLMRFVVIDKTETGNESTHLNSLSSIALIISGYLTILIVLDNVLTLPTWA 240
           L+ILPAL+TLLLM FVVIDKTETGNES HLNS S+IALIIS YLTILI+L+N LTLP WA
Sbjct: 181 LSILPALSTLLLMAFVVIDKTETGNESNHLNSFSTIALIISAYLTILIILNNALTLPIWA 240

Query: 241 RLFTFILLLALLASPLGIAVRAQTEDSVFKTKLQNTDDSVEYHRIPSEDQRNEQLTIVTD 300
           R+FTF+LLL L+ASPLGIA+RA+TEDSVF   LQN DDSVEY +IPSE Q ++QL IV+D
Sbjct: 241 RIFTFVLLLTLIASPLGIAIRARTEDSVF---LQNADDSVEYRQIPSEVQSDDQLAIVSD 300

Query: 301 GEMNVIEAIGTVNFWILFFAMMCGMGSGLATINNMNQLGQSLGYGTVEINTFVSLWSIWN 360
           GEMN+IEAIGTVNFW+L FAMMCGMGSGLATINNMNQLGQSLGYGTVEIN FVSLWSIWN
Sbjct: 301 GEMNIIEAIGTVNFWLLSFAMMCGMGSGLATINNMNQLGQSLGYGTVEINAFVSLWSIWN 360

Query: 361 FLGRLGSGYASDLLLWKLGWARPLLMAIALLTMSVGHIIIASGFSGNLYLGSVIVGICYG 420
           FLGRLG GYASDLLL +LG ARP LMA ALLTMS+GHIIIASGF GNLY+GSVIVGICYG
Sbjct: 361 FLGRLGPGYASDLLLRQLGLARPFLMAAALLTMSIGHIIIASGFPGNLYVGSVIVGICYG 420

Query: 421 AQWSLMPAIPAEIFGIRHMGTIYNTITVASPIGSYVLSVRVIGYIYDRE--ASAGDNSCS 480
           +QWSLMPAI AEIFGIR+MGTI+NTITVASPI SY+LSVRVIGY YDRE  ASAGDNSCS
Sbjct: 421 SQWSLMPAITAEIFGIRNMGTIFNTITVASPIASYILSVRVIGYTYDREAGASAGDNSCS 480

Query: 481 GKHCFITSFLIMAAVAFLGFVVAVALFFRTRRFYQVLVQRRLKD 523
           GKHCF+TSFL+MAAVAFLGF+VAV LFFRTRRFYQ LVQRRLKD
Sbjct: 481 GKHCFMTSFLVMAAVAFLGFLVAVVLFFRTRRFYQSLVQRRLKD 521

BLAST of Spg021929 vs. ExPASy Swiss-Prot
Match: F4I9E1 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 SV=1)

HSP 1 Score: 120.6 bits (301), Expect = 5.5e-26
Identity = 136/546 (24.91%), Postives = 244/546 (44.69%), Query Frame = 0

Query: 9   RWIATVASIWIQCICGPSYTFGIYSSALKSTQNYHQSTLDTVSVFKDIGATAGVLAGLLY 68
           +W   VA+IWIQ   G ++ F  YSS LKS     Q  L+ ++V  D+G   G  +G+  
Sbjct: 43  KWTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQVRLNYLAVASDLGKAFGWSSGIAL 102

Query: 69  SAVVSTDRPRRPWIVLTVGAIQCFLGYIFMWAAVSGLIPRPPVPAMCLFMFLAVHAQVYF 128
                +       +VL   A   F+GY   W  ++ +I   P   + L   LA  +  +F
Sbjct: 103 GYFPLS-------VVLFAAAAMGFVGYGVQWLVITNIITL-PYSLVFLCCLLAGLSICWF 162

Query: 129 NTANVVTGVHNFQLYGGTIVGILKGFLGLSGAVLIQFSNTFYNGNPVNYLLMLAILPALT 188
           NTA  +  + +F       + +   F G+S A+     N     +   YLL+ +++P + 
Sbjct: 163 NTACFILCIRHFPNNRALALSLTVSFNGISAALYSLAFNAINPSSSNLYLLLNSLVPLVV 222

Query: 189 TLLLMRFVV----IDKTETGNESTHLNSLSSIALIISGYLTILIVLDNVLTLPTWARLFT 248
           +   +  V+    +D T   +   H + + +I  +++   +  ++L +  T  + ARL  
Sbjct: 223 SFAALYPVLTKPSLDTTPDYDSRRHDSHVFTILNVLAVITSFHLLLSSSST--SSARL-N 282

Query: 249 FILLLALLASPLGIAVRAQTED---SVFKTKLQNTDDSVEYHRIPSEDQRNEQLTIV--- 308
           FI  + LL  PL   +     D    V   +L +  +S  Y  +  ++ +N++ ++    
Sbjct: 283 FIGAVVLLVFPLCAPLLVYARDYFLPVINARLNH--ESSGYVMLNIDELKNQKTSVSSKT 342

Query: 309 ------TDGEMNVIE---------AIGTVNFWILFFAMMCGMGSGLATINNMNQLGQSLG 368
                 T  E N +           I  + FW+ + A  CG   GL   NN+ Q+ QSLG
Sbjct: 343 GYEHMGTAKEGNTVRLGDEHSFRLLISRLEFWLYYIAYFCGGTIGLVYSNNLGQIAQSLG 402

Query: 369 YGTVEINTFVSLWSIWNFLGRLGSGYASDLLLWKLGWARPLLMAIALLTMSVGHIIIASG 428
             +    T V+++S ++F GRL S  A D +  +    R    AIALL   +   ++A  
Sbjct: 403 QNS---TTLVTIYSSFSFFGRLLSA-APDFMHKRFRLTRTGWFAIALLPTPIAFFLLAVS 462

Query: 429 FSGN--LYLGSVIVGICYGAQWSLMPAIPAEIFGIRHMGTIYNTITVASPIGSYVLSVRV 488
            S    L   + ++G+  G  ++   +I +++FG   +G  +N +    PIGS +L   +
Sbjct: 463 SSQQTALQTATALIGLSSGFIFAAAVSITSDLFGPNSVGVNHNILITNIPIGS-LLYGYI 522

Query: 489 IGYIYDREAS-------AGDNSCSGKHCFITSFLIMAAVAFLGFVVAVALFFRTRRFYQV 521
              IY+  AS       +    C G+ C+  +F+    ++ LG V +++L+ RT+  Y  
Sbjct: 523 AASIYEANASPDITPIVSDSIVCIGRDCYFKTFVFWGCLSILGVVSSLSLYIRTKPVYHR 570

BLAST of Spg021929 vs. ExPASy Swiss-Prot
Match: Q6CGU8 (Probable transporter MCH1 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) OX=284591 GN=MCH1 PE=3 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 9.3e-10
Identity = 54/188 (28.72%), Postives = 100/188 (53.19%), Query Frame = 0

Query: 336 NQLGQSLGYGTVE------INTFVSLWSIWNFLGRLGSGYASDLLLWKLGWARPLLMAIA 395
           N +G  L   TVE       +T VSL++ ++ + RL  G++S+ +  +   +RP+L+++ 
Sbjct: 293 NNMGAILDTVTVENADSPSFSTHVSLFATFSTVSRLVVGFSSEAM--ESHVSRPVLLSVI 352

Query: 396 LLTMSVGHIIIASG----FSGNLYLG--SVIVGICYGAQWSLMPAIPAEIFGIRHMGTIY 455
            L  +  H+++ SG    F    Y    +++ G  YG+ ++L+P I  +++GI ++GTI+
Sbjct: 353 ALVAACIHLMVPSGIFTVFDNAKYFSVVTIVNGFSYGSSFTLVPTIVTKVWGIANLGTIW 412

Query: 456 NTITVASPIGSYVLSVRVIGYIYDREASAGDNS----CSGKHCFITSFLIM-AAVAFLGF 507
            +  +A  +GS    + +   +YD  +  G  S    CSG HC+  +F+I    +AF   
Sbjct: 413 GSFILALAVGSLGYGL-LFAKVYDAASEVGVGSMSQVCSGVHCYGLTFVITGTGLAF--- 472

BLAST of Spg021929 vs. ExPASy Swiss-Prot
Match: Q6FWD4 (Probable transporter MCH1 OS=Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65) OX=284593 GN=MCH1 PE=3 SV=1)

HSP 1 Score: 53.1 bits (126), Expect = 1.1e-05
Identity = 60/249 (24.10%), Postives = 107/249 (42.97%), Query Frame = 0

Query: 276 TDDSVEYHRIPSEDQRNEQLTIVTDGEMNVIEAIGTVNFWILFFAMMCGMGSGLATINNM 335
           +D+ VE H   +E  R    T     +M +++       +I    + C +G     I NM
Sbjct: 244 SDNDVENHYDDNEQSRLLHATHAQ--QMTLMKVFRDPVLYIFGATIFCALGPLEMFIANM 303

Query: 336 NQLGQSL--GYGTVEINTFVSLWSIWNFLGRLGSGYASDLL---LWKLGWARPLLMAIAL 395
             L   L  G+     +  +S++++ + L RLG+G   D        + W   L + + L
Sbjct: 304 GSLTNVLAGGHEPAMSSALLSIYALTSTLTRLGTGLTVDYFNKRQLSVKWILLLFLVVGL 363

Query: 396 LTMSVGHIIIASG--------FSGNLYLGSVIVGICYGAQWSLMPAIPAEIFGIRHMGTI 455
           +T    +++  S          +  L+   ++ GI YG  +++ P I   ++G +  GT 
Sbjct: 364 VTQGKIYMLSMSSLDHSHMVTINRKLFYIGIMQGIAYGGLFTIYPTITLMVWGEKMFGTA 423

Query: 456 YNTITVASPIGSYVLSVRVIGYIYDRE-ASAGDNSCSGKHCFITSFLIMAAVAFLGFVVA 511
           Y T+ +A  +GS  LS  +   +YD E A++   SC       T+    AA+      V 
Sbjct: 424 YGTLMIAPALGS-ALSCLIYADVYDSECANSTTRSCIAPVYETTALEFCAAIL---LTVV 483

BLAST of Spg021929 vs. ExPASy Swiss-Prot
Match: A0LNN5 (L-lactate transporter OS=Syntrophobacter fumaroxidans (strain DSM 10017 / MPOB) OX=335543 GN=Sfum_3364 PE=1 SV=1)

HSP 1 Score: 50.4 bits (119), Expect = 6.9e-05
Identity = 52/203 (25.62%), Postives = 85/203 (41.87%), Query Frame = 0

Query: 307 EAIGTVNFWILFFAMMCGMGSGLATINNMNQLGQSLGYGTVEINTFVSLWSIWNFLGRLG 366
           EA G   FW+L+ A  CG  +GL  I ++   G+  G   +     VS  +  N   R+ 
Sbjct: 223 EAKGDTKFWLLYLAYFCGSFAGLMVIGHLAGFGRDAGLTAMAAAGAVSSLAFSNAATRIL 282

Query: 367 SGYASDLLLWKLGWARPLLMAIALLTMSVGHIIIASGFSGNLYLGSVIVGICYGAQWSLM 426
           SG+  D    K+G         AL T ++  I    G    L + ++++G  YGA ++L 
Sbjct: 283 SGWFVD----KIGIRVYFAALFALQTAAMIAIFQLGGSVVGLSIVAIVIGWNYGAMFTLF 342

Query: 427 PAIPAEIFGIRHMGTIYNTITVASPIGSYVLSVRVIGYIYDREASAGDNSCSGKHCFITS 486
           PA   + +G    G+ Y  +  A  +  +     V G++ D   +           +   
Sbjct: 343 PATCLQFYGPTAQGSNYGLLFTACGLAGFA-GPWVGGWLKDTTGT-----------YYLP 402

Query: 487 FLIMAAVAFLGFVVAVALFFRTR 510
           FL  AA+  LG     A+ F T+
Sbjct: 403 FLCAAALCALG----TAIVFMTK 405

BLAST of Spg021929 vs. ExPASy TrEMBL
Match: A0A6J1EZJ6 (uncharacterized protein LOC111440723 OS=Cucurbita moschata OX=3662 GN=LOC111440723 PE=4 SV=1)

HSP 1 Score: 882.9 bits (2280), Expect = 6.7e-253
Identity = 454/501 (90.62%), Postives = 483/501 (96.41%), Query Frame = 0

Query: 22  ICGPSYTFGIYSSALKSTQNYHQSTLDTVSVFKDIGATAGVLAGLLYSAVVSTDRPRRPW 81
           ICGPSYTFGIYSSALKS+QNY QSTLDTVSVFKDIGATAGVLAGLLYSAVVSTDRPRRPW
Sbjct: 13  ICGPSYTFGIYSSALKSSQNYDQSTLDTVSVFKDIGATAGVLAGLLYSAVVSTDRPRRPW 72

Query: 82  IVLTVGAIQCFLGYIFMWAAVSGLIPRPPVPAMCLFMFLAVHAQVYFNTANVVTGVHNFQ 141
           IVL+VGAIQCFLGYIF+WAAVSGLIPRPPV AMCLFMFLAVHAQV+FNTANVVTGVHNFQ
Sbjct: 73  IVLSVGAIQCFLGYIFIWAAVSGLIPRPPVSAMCLFMFLAVHAQVFFNTANVVTGVHNFQ 132

Query: 142 LYGGTIVGILKGFLGLSGAVLIQFSNTFYNGNPVNYLLMLAILPALTTLLLMRFVVIDKT 201
           LYGGTI+GILKGFLGLSGAVLIQFSNTFY+G+P++YLLMLAILPALTT+LLMRFVVI+KT
Sbjct: 133 LYGGTIIGILKGFLGLSGAVLIQFSNTFYSGDPISYLLMLAILPALTTVLLMRFVVIEKT 192

Query: 202 ETGNESTHLNSLSSIALIISGYLTILIVLDNVLTLPTWARLFTFILLLALLASPLGIAVR 261
           E GNESTHLNSLS IALIIS YLTILI+LDNV  LPTW RLFTF+LLL LLASPLGIA R
Sbjct: 193 EIGNESTHLNSLSKIALIISCYLTILIILDNVFFLPTWVRLFTFVLLLILLASPLGIANR 252

Query: 262 AQTEDSVFKTKLQNTDDSVEYHRIPSEDQRNEQLTIVTDGEMNVIEAIGTVNFWILFFAM 321
           AQTEDSV KTKLQ+TD+SVEYH+IP E+QRN+QL +VT+GEMNVIEAIGTVNFW+LF AM
Sbjct: 253 AQTEDSVSKTKLQSTDNSVEYHQIPGEEQRNDQLMMVTNGEMNVIEAIGTVNFWLLFSAM 312

Query: 322 MCGMGSGLATINNMNQLGQSLGYGTVEINTFVSLWSIWNFLGRLGSGYASDLLLWKLGWA 381
           MCGMGSGLATINNMNQLGQSLGY TVEI+TFVSLWSIWNFLGRLGSGYASDLLL +LGWA
Sbjct: 313 MCGMGSGLATINNMNQLGQSLGYKTVEIHTFVSLWSIWNFLGRLGSGYASDLLLRELGWA 372

Query: 382 RPLLMAIALLTMSVGHIIIASGFSGNLYLGSVIVGICYGAQWSLMPAIPAEIFGIRHMGT 441
           RPLLMA+ALLTMSVGHIIIASGFSGNLYLGSVIVGICYG+QWSLMPAI AEIFGIRHMGT
Sbjct: 373 RPLLMAVALLTMSVGHIIIASGFSGNLYLGSVIVGICYGSQWSLMPAITAEIFGIRHMGT 432

Query: 442 IYNTITVASPIGSYVLSVRVIGYIYDREASAGDNSCSGKHCFITSFLIMAAVAFLGFVVA 501
           IYNTITVASPIGSYVLSVRVIGYIYDREASAGDNSCSGKHCF+TSFLIMAAVAFLGF+VA
Sbjct: 433 IYNTITVASPIGSYVLSVRVIGYIYDREASAGDNSCSGKHCFMTSFLIMAAVAFLGFIVA 492

Query: 502 VALFFRTRRFYQVLVQRRLKD 523
           VALF RTRRFYQ+LVQR+LKD
Sbjct: 493 VALFLRTRRFYQLLVQRQLKD 513

BLAST of Spg021929 vs. ExPASy TrEMBL
Match: A0A6J1BYJ5 (LOW QUALITY PROTEIN: uncharacterized protein LOC111006908 OS=Momordica charantia OX=3673 GN=LOC111006908 PE=4 SV=1)

HSP 1 Score: 847.4 bits (2188), Expect = 3.1e-242
Identity = 440/524 (83.97%), Postives = 474/524 (90.46%), Query Frame = 0

Query: 1   MEAPSLSNRWIATVASIWIQCICGPSYTFGIYSSALKSTQNYHQSTLDTVSVFKDIGATA 60
           ME  SLSNRWIATVASIWIQCICGPSYTFGIYSSALKSTQ Y QSTLDTVSVF D+GATA
Sbjct: 1   MEVHSLSNRWIATVASIWIQCICGPSYTFGIYSSALKSTQAYDQSTLDTVSVFXDVGATA 60

Query: 61  GVLAGLLYSAVVSTDRPRRPWIVLTVGAIQCFLGYIFMWAAVSGLIPRPPVPAMCLFMFL 120
           GVLA LLYSAV S +RPRR WI+ TVGAIQCF GYIF+WAAVSGLIPRPPVPAMC FMFL
Sbjct: 61  GVLAVLLYSAVASNNRPRRSWIIHTVGAIQCFAGYIFIWAAVSGLIPRPPVPAMCFFMFL 120

Query: 121 AVHAQVYFNTANVVTGVHNFQLYGGTIVGILKGFLGLSGAVLIQFSNTFYNGNPVNYLLM 180
           AVHAQV+FNTANVVTGVHNFQLY  TIVGILKGF GL GAVLIQFSNTF N +P N L++
Sbjct: 121 AVHAQVFFNTANVVTGVHNFQLYSATIVGILKGFAGLXGAVLIQFSNTFCNRDPANNLVL 180

Query: 181 LAILPALTTLLLMRFVVIDKTETGNESTHLNSLSSIALIISGYLTILIVLDNVLTLPTWA 240
           L+ILPAL+TLLLM FVVIDKTETGNES HLNS S+IALIIS YLTILI+L+N LTLP WA
Sbjct: 181 LSILPALSTLLLMAFVVIDKTETGNESNHLNSFSTIALIISAYLTILIILNNALTLPIWA 240

Query: 241 RLFTFILLLALLASPLGIAVRAQTEDSVFKTKLQNTDDSVEYHRIPSEDQRNEQLTIVTD 300
           R+FTF+LLL L+ASPLGIA+RA+TEDSVF   LQN DDSVEY +IPSE Q ++QL IV+D
Sbjct: 241 RIFTFVLLLTLIASPLGIAIRARTEDSVF---LQNADDSVEYRQIPSEVQSDDQLAIVSD 300

Query: 301 GEMNVIEAIGTVNFWILFFAMMCGMGSGLATINNMNQLGQSLGYGTVEINTFVSLWSIWN 360
           GEMN+IEAIGTVNFW+L FAMMCGMGSGLATINNMNQLGQSLGYGTVEIN FVSLWSIWN
Sbjct: 301 GEMNIIEAIGTVNFWLLSFAMMCGMGSGLATINNMNQLGQSLGYGTVEINAFVSLWSIWN 360

Query: 361 FLGRLGSGYASDLLLWKLGWARPLLMAIALLTMSVGHIIIASGFSGNLYLGSVIVGICYG 420
           FLGRLG GYASDLLL +LG ARP LMA ALLTMS+GHIIIASGF GNLY+GSVIVGICYG
Sbjct: 361 FLGRLGPGYASDLLLRQLGLARPFLMAAALLTMSIGHIIIASGFPGNLYVGSVIVGICYG 420

Query: 421 AQWSLMPAIPAEIFGIRHMGTIYNTITVASPIGSYVLSVRVIGYIYDRE--ASAGDNSCS 480
           +QWSLMPAI AEIFGIR+MGTI+NTITVASPI SY+LSVRVIGY YDRE  ASAGDNSCS
Sbjct: 421 SQWSLMPAITAEIFGIRNMGTIFNTITVASPIASYILSVRVIGYTYDREAGASAGDNSCS 480

Query: 481 GKHCFITSFLIMAAVAFLGFVVAVALFFRTRRFYQVLVQRRLKD 523
           GKHCF+TSFL+MAAVAFLGF+VAV LFFRTRRFYQ LVQRRLKD
Sbjct: 481 GKHCFMTSFLVMAAVAFLGFLVAVVLFFRTRRFYQSLVQRRLKD 521

BLAST of Spg021929 vs. ExPASy TrEMBL
Match: A0A5A7UT75 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold350G002270 PE=4 SV=1)

HSP 1 Score: 679.1 bits (1751), Expect = 1.5e-191
Identity = 352/544 (64.71%), Postives = 426/544 (78.31%), Query Frame = 0

Query: 1   MEAPS-LSNRWIATVASIWIQCICGPSYTFGIYSSALKSTQNYHQSTLDTVSVFKDIGAT 60
           MEA + + N+W ATV  IWIQCICG SYTF IYSSALKSTQ+Y QSTLDTVSVFKDIGA 
Sbjct: 1   MEASNFMHNKWTATVLGIWIQCICGASYTFSIYSSALKSTQSYDQSTLDTVSVFKDIGAN 60

Query: 61  AGVLAGLLYSAVVSTDRPRR----PWIVLTVGAIQCFLGYIFMWAAVSGLIPRPPVPAMC 120
           AG+++G LYSAV   + PRR    PW+V   GAIQ FLGYIF+WAAVSG+I RPPVPAMC
Sbjct: 61  AGIISGFLYSAVTPLN-PRRAFAGPWMVHAAGAIQWFLGYIFIWAAVSGVIDRPPVPAMC 120

Query: 121 LFMFLAVHAQVYFNTANVVTGVHNFQLYGGTIVGILKGFLGLSGAVLIQFSNTFYNGNPV 180
            FMFLA HAQ +FNTANVVTGVHNF  Y GTIVGI+KG+LGLSGA+LIQ  NT  N +P 
Sbjct: 121 FFMFLAAHAQTFFNTANVVTGVHNFANYSGTIVGIMKGYLGLSGALLIQVYNTTCNADPS 180

Query: 181 NYLLMLAILPALTTLLLMRFVVIDKTETGNESTHLNSLSSIALIISGYLTILIVLDNVLT 240
           N+LLMLA+LP + +++ M FV IDKTE+ NE  HLNSLS++A+I++ YL ++I+L+N  +
Sbjct: 181 NFLLMLAVLPTVLSIMFMWFVRIDKTESKNEMKHLNSLSALAVIVAFYLMVVIILNNAFS 240

Query: 241 LPTWARLFTFILLLALLASPLGIAVRAQTED--------------SVFKTKLQNTDDSVE 300
           L +W R FTF +LL LLA+PLGIA+ AQ ED               V K +  + +DSVE
Sbjct: 241 LSSWTRYFTFSILLILLAAPLGIAINAQKEDFRGSPPSLITEESHVVNKPEPIDAEDSVE 300

Query: 301 YHRIPSEDQRNEQLTIVTDGE----MNVIEAIGTVNFWILFFAMMCGMGSGLATINNMNQ 360
           YH +P E+   +Q+ +V++      MNV+EAI T+NFW+LF AM+CGMGSGLATINNM+Q
Sbjct: 301 YHELPREE---DQIMVVSNSRAPQPMNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQ 360

Query: 361 LGQSLGYGTVEINTFVSLWSIWNFLGRLGSGYASDLLLWKLGWARPLLMAIALLTMSVGH 420
           LGQSLGY   E  TFVSLWSIWNFLGR G+GY SD L    GWARPLLMAI LL MS GH
Sbjct: 361 LGQSLGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLFHTYGWARPLLMAITLLIMSGGH 420

Query: 421 IIIASGFSGNLYLGSVIVGICYGAQWSLMPAIPAEIFGIRHMGTIYNTITVASPIGSYVL 480
           I+IASGFSGNLY+GS++VGICYG+QWSLMP I +EIFG+ HMGTI+NTI +ASP+GSY+ 
Sbjct: 421 IVIASGFSGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIF 480

Query: 481 SVRVIGYIYDREASAGDNSCSGKHCFITSFLIMAAVAFLGFVVAVALFFRTRRFYQVLVQ 522
           SVRVIGYIYDREA+    +CSG HCF+ SFL+MA VAFLGF+VA ALFFRTRRFY++ +Q
Sbjct: 481 SVRVIGYIYDREAAGEHGACSGTHCFVVSFLVMAIVAFLGFLVAAALFFRTRRFYRLALQ 540

BLAST of Spg021929 vs. ExPASy TrEMBL
Match: A0A1S3BWC2 (protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo OX=3656 GN=LOC103493962 PE=4 SV=1)

HSP 1 Score: 679.1 bits (1751), Expect = 1.5e-191
Identity = 352/544 (64.71%), Postives = 426/544 (78.31%), Query Frame = 0

Query: 1   MEAPS-LSNRWIATVASIWIQCICGPSYTFGIYSSALKSTQNYHQSTLDTVSVFKDIGAT 60
           MEA + + N+W ATV  IWIQCICG SYTF IYSSALKSTQ+Y QSTLDTVSVFKDIGA 
Sbjct: 1   MEASNFMHNKWTATVLGIWIQCICGASYTFSIYSSALKSTQSYDQSTLDTVSVFKDIGAN 60

Query: 61  AGVLAGLLYSAVVSTDRPRR----PWIVLTVGAIQCFLGYIFMWAAVSGLIPRPPVPAMC 120
           AG+++G LYSAV   + PRR    PW+V   GAIQ FLGYIF+WAAVSG+I RPPVPAMC
Sbjct: 61  AGIISGFLYSAVTPLN-PRRAFAGPWMVHAAGAIQWFLGYIFIWAAVSGVIDRPPVPAMC 120

Query: 121 LFMFLAVHAQVYFNTANVVTGVHNFQLYGGTIVGILKGFLGLSGAVLIQFSNTFYNGNPV 180
            FMFLA HAQ +FNTANVVTGVHNF  Y GTIVGI+KG+LGLSGA+LIQ  NT  N +P 
Sbjct: 121 FFMFLAAHAQTFFNTANVVTGVHNFANYSGTIVGIMKGYLGLSGALLIQVYNTTCNADPS 180

Query: 181 NYLLMLAILPALTTLLLMRFVVIDKTETGNESTHLNSLSSIALIISGYLTILIVLDNVLT 240
           N+LLMLA+LP + +++ M FV IDKTE+ NE  HLNSLS++A+I++ YL ++I+L+N  +
Sbjct: 181 NFLLMLAVLPTVLSIMFMWFVRIDKTESKNEMKHLNSLSALAVIVAFYLMVVIILNNAFS 240

Query: 241 LPTWARLFTFILLLALLASPLGIAVRAQTED--------------SVFKTKLQNTDDSVE 300
           L +W R FTF +LL LLA+PLGIA+ AQ ED               V K +  + +DSVE
Sbjct: 241 LSSWTRYFTFSILLILLAAPLGIAINAQKEDFRGSPPSLITEESHVVNKPEPIDAEDSVE 300

Query: 301 YHRIPSEDQRNEQLTIVTDGE----MNVIEAIGTVNFWILFFAMMCGMGSGLATINNMNQ 360
           YH +P E+   +Q+ +V++      MNV+EAI T+NFW+LF AM+CGMGSGLATINNM+Q
Sbjct: 301 YHELPREE---DQIMVVSNSRAPQPMNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQ 360

Query: 361 LGQSLGYGTVEINTFVSLWSIWNFLGRLGSGYASDLLLWKLGWARPLLMAIALLTMSVGH 420
           LGQSLGY   E  TFVSLWSIWNFLGR G+GY SD L    GWARPLLMAI LL MS GH
Sbjct: 361 LGQSLGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLFHTYGWARPLLMAITLLIMSGGH 420

Query: 421 IIIASGFSGNLYLGSVIVGICYGAQWSLMPAIPAEIFGIRHMGTIYNTITVASPIGSYVL 480
           I+IASGFSGNLY+GS++VGICYG+QWSLMP I +EIFG+ HMGTI+NTI +ASP+GSY+ 
Sbjct: 421 IVIASGFSGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIF 480

Query: 481 SVRVIGYIYDREASAGDNSCSGKHCFITSFLIMAAVAFLGFVVAVALFFRTRRFYQVLVQ 522
           SVRVIGYIYDREA+    +CSG HCF+ SFL+MA VAFLGF+VA ALFFRTRRFY++ +Q
Sbjct: 481 SVRVIGYIYDREAAGEHGACSGTHCFVVSFLVMAIVAFLGFLVAAALFFRTRRFYRLALQ 540

BLAST of Spg021929 vs. ExPASy TrEMBL
Match: A0A6J1IA87 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111471070 PE=4 SV=1)

HSP 1 Score: 677.6 bits (1747), Expect = 4.3e-191
Identity = 347/534 (64.98%), Postives = 414/534 (77.53%), Query Frame = 0

Query: 6   LSNRWIATVASIWIQCICGPSYTFGIYSSALKSTQNYHQSTLDTVSVFKDIGATAGVLAG 65
           ++N+W+AT   IWIQCICG SYTF IYSSALKSTQ Y QSTLDTVSVFKDIGA AGV++G
Sbjct: 7   MTNKWVATAIGIWIQCICGASYTFSIYSSALKSTQGYDQSTLDTVSVFKDIGANAGVISG 66

Query: 66  LLYSAVVSTDRPRR---PWIVLTVGAIQCFLGYIFMWAAVSGLIPRPPVPAMCLFMFLAV 125
            LYSAV    R R    PW+V   GAIQCFLGYIF+WAAVSGLI RPPVPAMC FMFLA 
Sbjct: 67  FLYSAVTPASRRRSSVGPWVVHAAGAIQCFLGYIFIWAAVSGLIDRPPVPAMCFFMFLAA 126

Query: 126 HAQVYFNTANVVTGVHNFQLYGGTIVGILKGFLGLSGAVLIQFSNTFYNGNPVNYLLMLA 185
           HAQ +FNTANVVTGVHNF  + GTIVGI+KG+LGLSGA+LIQ  NT  NG+P N++LMLA
Sbjct: 127 HAQTFFNTANVVTGVHNFAHHSGTIVGIMKGYLGLSGALLIQVYNTACNGDPSNFVLMLA 186

Query: 186 ILPALTTLLLMRFVVIDKTETGNESTHLNSLSSIALIISGYLTILIVLDNVLTLPTWARL 245
           + P L +++ M FV ID+TE+ NE  HLNSLS++A+I++ YL ++I+ +N  +L +W R 
Sbjct: 187 VFPTLLSIMFMWFVRIDRTESRNEMKHLNSLSALAVIVAFYLMVVIIFNNTFSLSSWTRF 246

Query: 246 FTFILLLALLASPLGIAVRAQTED------SVF--------KTKLQNTDDSVEYHRIPSE 305
            TF +LL LL +PLGIA+ AQ ED      S F        K K  N +DSVEYH +PS+
Sbjct: 247 VTFSILLVLLVAPLGIAINAQREDFRMSSPSFFTEENPVLNKPKSANAEDSVEYHELPSD 306

Query: 306 D-QRNEQLTIVTDGEMNVIEAIGTVNFWILFFAMMCGMGSGLATINNMNQLGQSLGYGTV 365
           + Q            MNV+EA+ T+NFW+LF AM+CGMGSGLATINNM+QLGQSLGY   
Sbjct: 307 ELQTMAAANSRAPRAMNVLEALRTLNFWLLFVAMVCGMGSGLATINNMSQLGQSLGYTAT 366

Query: 366 EINTFVSLWSIWNFLGRLGSGYASDLLLWKLGWARPLLMAIALLTMSVGHIIIASGFSGN 425
           E  TFVSLWSIWNFLGR G+GY SD LL   GWARPLLMAI LL MS GHI+IASGFSGN
Sbjct: 367 ETKTFVSLWSIWNFLGRFGAGYTSDFLLHSYGWARPLLMAITLLIMSGGHIVIASGFSGN 426

Query: 426 LYLGSVIVGICYGAQWSLMPAIPAEIFGIRHMGTIYNTITVASPIGSYVLSVRVIGYIYD 485
           LY+GS++VGICYG+QWSLMP I +EIFG+ HMGTI+NTI +ASP+GSY+ SVRVIGYIYD
Sbjct: 427 LYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRVIGYIYD 486

Query: 486 REASAGDNSCSGKHCFITSFLIMAAVAFLGFVVAVALFFRTRRFYQVLVQRRLK 522
           REA+    SCSG HCF+ SFL+MA VAFLG +VA ALFFRTRRFY++ +QRR++
Sbjct: 487 REAAEKHGSCSGTHCFVVSFLVMAIVAFLGSLVAAALFFRTRRFYRLALQRRVE 540

BLAST of Spg021929 vs. TAIR 10
Match: AT1G74780.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 585.9 bits (1509), Expect = 3.3e-167
Identity = 307/528 (58.14%), Postives = 390/528 (73.86%), Query Frame = 0

Query: 6   LSNRWIATVASIWIQCICGPSYTFGIYSSALKSTQNYHQSTLDTVSVFKDIGATAGVLAG 65
           L  +W+A  ASIWIQC  G SYTFGIYS+ LKSTQ+Y QSTLDTVSVFKDIGA AGV +G
Sbjct: 4   LRTKWVAMTASIWIQCASGASYTFGIYSAVLKSTQSYDQSTLDTVSVFKDIGANAGVFSG 63

Query: 66  LLYSAVVSTDRPRR---------PWIVLTVGAIQCFLGYIFMWAAVSGLIPRPPVPAMCL 125
           LLY+   S     R         PW+VL VGAIQCF GY  +WA+V+GLI +PPVP MCL
Sbjct: 64  LLYTYATSNRLRGRGGGIGGAGGPWVVLAVGAIQCFAGYFLIWASVTGLIRKPPVPLMCL 123

Query: 126 FMFLAVHAQVYFNTANVVTGVHNFQLYGGTIVGILKGFLGLSGAVLIQFSNTFYNGNPVN 185
           FMFLA  +Q +FNTANVV+ V NF  YGGT VGI+KGFLGLSGA+LIQ   T   G+P +
Sbjct: 124 FMFLAAQSQTFFNTANVVSAVENFADYGGTAVGIMKGFLGLSGAILIQLYETLCAGDPAS 183

Query: 186 YLLMLAILPALTTLLLMRFVVIDKTETGNESTHLNSLSSIALIISGYLTILIVLDNVLTL 245
           ++L+LA+ P + +LL+M  V I +T   ++  HLN LS+++LII+ YL I+I+L N   L
Sbjct: 184 FILLLAVTPTVLSLLVMPLVRIYETSVADDKKHLNGLSAVSLIIAAYLMIIIILKNTFGL 243

Query: 246 PTWARLFTFILLLALLASPLGIAVRAQTEDSVFKTKLQNTDDSVEYHR-IPSEDQRNEQL 305
            +WA + T + LL +LA PL IA RAQ  D + KT   +    +   +   S +Q +E  
Sbjct: 244 SSWANIVTLVCLLVMLALPLLIARRAQ-RDGMEKTVPHDYSPLISSPKATTSGNQSSEGD 303

Query: 306 TIVTDG---EMNVIEAIGTVNFWILFFAMMCGMGSGLATINNMNQLGQSLGYGTVEINTF 365
           + V  G    +N+++A+  ++FW+LF AM+CGMGSGL+TINN+ Q+G+SL Y +VEIN+ 
Sbjct: 304 SKVEAGLSENLNLLQAMKKLSFWLLFLAMICGMGSGLSTINNIRQIGESLRYSSVEINSL 363

Query: 366 VSLWSIWNFLGRLGSGYASDLLLWKLGWARPLLMAIALLTMSVGHIIIASGFSGNLYLGS 425
           VSLWSIWNFLGR G+GYASD LL K GW RPLLMA  L TMS+GH+IIASGF GNLY+GS
Sbjct: 364 VSLWSIWNFLGRFGAGYASDALLHKKGWPRPLLMAATLGTMSIGHLIIASGFQGNLYVGS 423

Query: 426 VIVGICYGAQWSLMPAIPAEIFGIRHMGTIYNTITVASPIGSYVLSVRVIGYIYDREASA 485
           VIVG+CYG+QWSLMP I +E+FGIRHMGTI+NTI+VASPIGSY+ SVR+IGYIYD+ AS 
Sbjct: 424 VIVGVCYGSQWSLMPTITSELFGIRHMGTIFNTISVASPIGSYIFSVRLIGYIYDKTASG 483

Query: 486 GDNSCSGKHCFITSFLIMAAVAFLGFVVAVALFFRTRRFYQVLVQRRL 521
             N+C G HCF  SF+IMA+VAF GF+VA+ LFFRT+  Y+ ++ +RL
Sbjct: 484 EGNTCYGSHCFRLSFIIMASVAFFGFLVAIVLFFRTKTLYRQILVKRL 530

BLAST of Spg021929 vs. TAIR 10
Match: AT1G18940.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 550.1 bits (1416), Expect = 2.0e-156
Identity = 283/533 (53.10%), Postives = 380/533 (71.29%), Query Frame = 0

Query: 1   MEAPSLSNRWIATVASIWIQCICGPSYTFGIYSSALKSTQNYHQSTLDTVSVFKDIGATA 60
           M    L  +W+A  ASIWIQC  G SYTFGIYS+ LKSTQ+Y QSTLDTVSVFKDIG   
Sbjct: 1   MATEILRTKWMAMTASIWIQCSAGGSYTFGIYSAILKSTQSYDQSTLDTVSVFKDIGGNV 60

Query: 61  GVLAGLLYSAVVSTDRPR-------RPWIVLTVGAIQCFLGYIFMWAAVSGLIPRPPVPA 120
           GVL+GL+Y+A     R R        PW+V+ +GAI  F GY  MWA+V+GLI RPPVP 
Sbjct: 61  GVLSGLVYTAATFNRRRRDGRERRGGPWVVILIGAILNFTGYFLMWASVTGLIKRPPVPV 120

Query: 121 MCLFMFLAVHAQVYFNTANVVTGVHNFQLYGGTIVGILKGFLGLSGAVLIQFSNTFYNGN 180
           MCLFMF+A  +  + NTANVV+ + NF  YGGT VGI+KGF+GLSGA+LIQ       G+
Sbjct: 121 MCLFMFIAAQSLTFLNTANVVSSLENFADYGGTAVGIMKGFVGLSGAMLIQLYEVVCPGD 180

Query: 181 PVNYLLMLAILPALTTLLLMRFVVIDKTETGNESTHLNSLSSIALIISGYLTILIVLDNV 240
           P  ++L+LAI+P+L ++L+M  V + KT T +E  HL+ LS+++LII+ YL I I+L + 
Sbjct: 181 PKTFILLLAIVPSLLSVLVMPLVRVYKTSTVDEKKHLDGLSTLSLIIAAYLMITIILKST 240

Query: 241 LTLPTWARLFTFILLLALLASPLGIAVRAQTE------DSVFKTKLQNTDDSVEYHRIPS 300
           L+LP+WA   T  +LL LL+SPL +AVRA  +       SV+   + N + +        
Sbjct: 241 LSLPSWANAVTLAVLLVLLSSPLLVAVRAHRDSIEKPLSSVYSPLVDNLEATTS------ 300

Query: 301 EDQRNEQLTIVTDGEMNVIEAIGTVNFWILFFAMMCGMGSGLATINNMNQLGQSLGYGTV 360
                E L +  D  +N+++A+  V+FW+LF AM+CGMGSG++TINN+ Q+G+SL Y +V
Sbjct: 301 ----GEILMLDEDKSLNLLQAMCNVDFWLLFLAMICGMGSGISTINNIRQIGESLRYTSV 360

Query: 361 EINTFVSLWSIWNFLGRLGSGYASDLLLWKLGWARPLLMAIALLTMSVGHIIIASGFSGN 420
           EIN+ ++LW+IWNF+GR G GY SD LL + GW RPLLMA  L TM++GH+IIASGF GN
Sbjct: 361 EINSLLALWNIWNFIGRFGGGYVSDWLLHRKGWPRPLLMATTLGTMTIGHLIIASGFQGN 420

Query: 421 LYLGSVIVGICYGAQWSLMPAIPAEIFGIRHMGTIYNTITVASPIGSYVLSVRVIGYIYD 480
           LY GS+IVGICYG+QWSLMP I +E+FG++HMGTIYNTI++ASP+GSY+ SVR+IGYIYD
Sbjct: 421 LYPGSIIVGICYGSQWSLMPTITSELFGVKHMGTIYNTISIASPMGSYIFSVRLIGYIYD 480

Query: 481 REASAGDNSCSGKHCFITSFLIMAAVAFLGFVVAVALFFRTRRFYQVLVQRRL 521
           R      N+C G HCF  +++++A+VAFLGF+V+  L FRT+  Y+ + ++ L
Sbjct: 481 RTIIGEGNTCYGPHCFRLAYVVIASVAFLGFLVSCVLVFRTKTIYRQIFEKIL 523

BLAST of Spg021929 vs. TAIR 10
Match: AT2G34350.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 547.7 bits (1410), Expect = 9.8e-156
Identity = 288/522 (55.17%), Postives = 375/522 (71.84%), Query Frame = 0

Query: 7   SNRWIATVASIWIQCICGPSYTFGIYSSALKSTQNYHQSTLDTVSVFKDIGATAGVLAGL 66
           + +W+A  ASIWIQ   G SYTFGIYSS LKS+Q+Y QSTLDTVSV+KDIGA  G+L+GL
Sbjct: 5   NTKWVAAAASIWIQSFSGASYTFGIYSSVLKSSQSYDQSTLDTVSVYKDIGANVGILSGL 64

Query: 67  LYSAVVSTDRPR-----RPWIVLTVGAIQCFLGYIFMWAAVSGLIPRPPVPAMCLFMFLA 126
            Y+AV S           PW+V+ VG +Q F+GY F+W A SG+IPRPPV  MCLFMF A
Sbjct: 65  FYTAVASRKSGNGGFFSGPWLVIFVGLLQWFVGYGFIWMATSGVIPRPPVAMMCLFMFFA 124

Query: 127 VHAQVYFNTANVVTGVHNFQLYGGTIVGILKGFLGLSGAVLIQFSNTFYNGNPVNYLLML 186
            H Q +FNTA VVT V NF  YGGT VGI+KG+LGLSGA+L+Q  + F  G+P NY+L+L
Sbjct: 125 GHCQPFFNTAIVVTAVRNFSDYGGTAVGIMKGYLGLSGAILVQMYHIFCGGDPRNYILLL 184

Query: 187 AILPALTTLLLMRFVVIDKTETGNESTHLNSLSSIALIISGYLTILIVLDNVLTLPTWAR 246
           A++P+L  L LM FV    T    +  HLN LS+I+LII  YL ++I+++N++ +    +
Sbjct: 185 AVVPSLLILTLMPFVRTYDTVIAGDKKHLNGLSAISLIIVTYLMVVILVENIIGMSMPMK 244

Query: 247 LFTFILLLALLASPLGIAVRAQTEDSVFKTKLQNTDDSVEYHRIPSEDQRNEQLTIVTD- 306
           + +F  LL LLASPL +AVRAQ E+   + +  + D  V       +  +    + V D 
Sbjct: 245 ICSFTFLLLLLASPLLVAVRAQREE---EHRFLSLDFPVTERTTLLDSPKLNSSSDVKDV 304

Query: 307 --GEMNVIEAIGTVNFWILFFAMMCGMGSGLATINNMNQLGQSLGYGTVEINTFVSLWSI 366
              +MNV+EAI T NFW+LF AM+CGMGSGLATINN+ Q+G+SL Y TV++N+ VSLWSI
Sbjct: 305 MTNDMNVLEAICTTNFWLLFVAMICGMGSGLATINNIRQMGESLRYSTVQLNSLVSLWSI 364

Query: 367 WNFLGRLGSGYASDLLLWKLGWARPLLMAIALLTMSVGHIIIASGFSGNLYLGSVIVGIC 426
           WNFLGR GSGY SD  L   GW RP+ MAI L  M++GHI++ASG  G+LY+GS++VG+ 
Sbjct: 365 WNFLGRFGSGYISDTYLHSHGWPRPVFMAITLGLMAIGHIVMASGLLGSLYIGSLLVGLA 424

Query: 427 YGAQWSLMPAIPAEIFGIRHMGTIYNTITVASPIGSYVLSVRVIGYIYDREASAGDNSCS 486
           YG+QWSLMP I +EIFG+ HMGTI+ TI++ASP+GSY  SV+VIGY+YD+ AS  D+SC 
Sbjct: 425 YGSQWSLMPTITSEIFGVLHMGTIFYTISIASPVGSYFFSVKVIGYLYDKVASEDDHSCY 484

Query: 487 GKHCFITSFLIMAAVAFLGFVVAVALFFRTRRFYQVLVQRRL 521
           G HCF TSFLIMAA+A LG +VA+ L  RT++FY  LV +R+
Sbjct: 485 GNHCFRTSFLIMAAMALLGSLVALVLLLRTKKFYATLVAKRI 523

BLAST of Spg021929 vs. TAIR 10
Match: AT2G34355.1 (Major facilitator superfamily protein )

HSP 1 Score: 542.0 bits (1395), Expect = 5.4e-154
Identity = 286/518 (55.21%), Postives = 375/518 (72.39%), Query Frame = 0

Query: 6   LSNRWIATVASIWIQCICGPSYTFGIYSSALKSTQNYHQSTLDTVSVFKDIGATAGVLAG 65
           ++ +W+A  ASIWIQ   G +YTF IYSS LKS+Q+Y QSTLD VSVFKDIG T G+++G
Sbjct: 4   INTKWVAAAASIWIQSFSGATYTFAIYSSILKSSQSYDQSTLDFVSVFKDIGGTFGIISG 63

Query: 66  LLYSAVVSTDRP-RRPWIVLTVGAIQCFLGYIFMWAAVSGLIPRPPVPAMCLFMFLAVHA 125
            LY+A+ S  R    PW+V+ VG +Q F+G+ F+WA+V GLI  PPVP MCLF+FLA H+
Sbjct: 64  FLYTAMTSKSRGFGGPWVVVFVGLVQWFVGFFFIWASVVGLIAPPPVPLMCLFVFLAGHS 123

Query: 126 QVYFNTANVVTGVHNFQLYGGTIVGILKGFLGLSGAVLIQFSNTFY--NGNPVNYLLMLA 185
             +FNTANVVT   NF  YGGT VGI++GFLGLSGA+LIQ  +      GNP  ++L+LA
Sbjct: 124 LPFFNTANVVTAARNFSQYGGTAVGIMQGFLGLSGAILIQLYHAVCGGEGNPATFILLLA 183

Query: 186 ILPALTTLLLMRFVVIDKTETGNESTHLNSLSSIALIISGYLTILIVLDNVLTLPTWARL 245
           I+P L   L M FV + +T T ++  HL+ LS+I++II+ YL ++I ++NVL L    ++
Sbjct: 184 IVPTLVMFLAMPFVRVYETVTISDKKHLDGLSAISMIIAAYLMVVITVENVLGLSRSMQI 243

Query: 246 FTFILLLALLASPLGIAVRAQTEDSVFKTKLQN-TDDSVEYHRIPSEDQRNEQLTIVTDG 305
           F+FIL+L LLASPL +AVRA  E     + L     D+      PS +   +   +V + 
Sbjct: 244 FSFILVLLLLASPLLVAVRALREKRQTLSSLDGPVLDTSALLDPPSSNIFPDGDHLVAE- 303

Query: 306 EMNVIEAIGTVNFWILFFAMMCGMGSGLATINNMNQLGQSLGYGTVEINTFVSLWSIWNF 365
           + N++EA+ TVNFW+LF AM+CGMGSG AT+NNM Q+G+SL Y +V++N+ VSLWSIWNF
Sbjct: 304 DSNILEAMSTVNFWLLFLAMLCGMGSGFATVNNMRQIGESLRYSSVQLNSLVSLWSIWNF 363

Query: 366 LGRLGSGYASDLLLWKLGWARPLLMAIALLTMSVGHIIIASGFSGNLYLGSVIVGICYGA 425
           LGR G+GY SD  L K  W RP+ MAI L  M++GHII+ASG  G+LY GSV++G+ YG+
Sbjct: 364 LGRFGAGYVSDTFLHKHSWPRPIFMAITLGVMAIGHIIVASGVQGSLYAGSVLIGMAYGS 423

Query: 426 QWSLMPAIPAEIFGIRHMGTIYNTITVASPIGSYVLSVRVIGYIYDREASAGDNSCSGKH 485
           QWSLMP I +EIFGIRHMGTIY TI++A PIGSY+LSV+VIGY YD+ AS  DNSC G  
Sbjct: 424 QWSLMPTITSEIFGIRHMGTIYFTISIAGPIGSYILSVKVIGYFYDKVASEDDNSCFGSQ 483

Query: 486 CFITSFLIMAAVAFLGFVVAVALFFRTRRFYQVLVQRR 520
           CF TSF+IMA+VA  G +VA  LFFRT +FY+ LV +R
Sbjct: 484 CFRTSFMIMASVALFGSLVASVLFFRTHKFYKNLVAKR 520

BLAST of Spg021929 vs. TAIR 10
Match: AT2G39210.1 (Major facilitator superfamily protein )

HSP 1 Score: 317.0 bits (811), Expect = 2.8e-86
Identity = 199/559 (35.60%), Postives = 308/559 (55.10%), Query Frame = 0

Query: 6   LSNRWIATVASIWIQCICGPSYTFGIYSSALKSTQNYHQSTLDTVSVFKDIGATAGVLAG 65
           L+ RW     S+ I    G +Y FGIYS  +K T  Y Q+TL+ +S FKD+GA  GVLAG
Sbjct: 18  LTGRWFMFFGSLLIMSTAGATYMFGIYSGDIKETLGYDQTTLNLLSFFKDLGANVGVLAG 77

Query: 66  LLYSAVVSTDRPRRPWIVLTVGAIQCFLGYIFMWAAVSGLIPRPPVPAMCLFMFLAVHAQ 125
           LL       +    PW +L +GAI  F GY  +W AV+  I +P V  MCL++ +  ++Q
Sbjct: 78  LL-------NEVTPPWFILLIGAILNFFGYFMIWLAVTERISKPQVWHMCLYICVGANSQ 137

Query: 126 VYFNTANVVTGVHNFQLYGGTIVGILKGFLGLSGAVLIQFSNTFYNGNPVNYLLMLAILP 185
            + NT ++VT V NF    G ++GILKG++GLSGA++ Q    FY  +    +LM+  LP
Sbjct: 138 SFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLYRAFYGEDTKELILMIGWLP 197

Query: 186 ALTTLLLMRFVVIDKTE-TGNESTHLNSLSSIALIISGYLTILIVLDNV--LTLPTWARL 245
           A+ +   +R + I K +   NE     +   I+L ++ +L ++I+++ +   T   +   
Sbjct: 198 AIVSFAFLRTIRIMKVKRQTNELKVFYNFLYISLGLATFLMVVIIINKLSGFTQSEFGGS 257

Query: 246 FTFILLLALLASPLGIAVRAQTEDSVFKTKLQNTDDSVEYHRIPSEDQRNEQLTIVTDGE 305
              +++L LL     I V    E  ++K K    +D    + +  + + +       DGE
Sbjct: 258 AAVVIVLLLLP----IIVVILEEKKLWKEKQVALNDPAPINVVTEKPKLDSSEFKDDDGE 317

Query: 306 ---------------------------MNVIEAIGTVNFWILFFAMMCGMGSGLATINNM 365
                                        +++A+ +V+  ILF A +CG+G  L  I+N+
Sbjct: 318 ESKEVVEKVKTPSCWTTVFNPPERGDDYTILQALFSVDMLILFLATICGVGGTLTAIDNL 377

Query: 366 NQLGQSLGYGTVEINTFVSLWSIWNFLGRLGSGYASDLLLWKLGWARPLLMAIALLTMSV 425
            Q+G SLGY    ++TFVSL SIWN+ GR+ SG  S++ L K  + RPL++ + LL    
Sbjct: 378 GQIGNSLGYPKRSVSTFVSLVSIWNYYGRVVSGVVSEIFLIKYKFPRPLMLTMVLLLSCA 437

Query: 426 GHIIIASGFSGNLYLGSVIVGICYGAQWSLMPAIPAEIFGIRHMGTIYNTITVASPIGSY 485
           GH++IA    G LY+ SVI+G C+GAQW L+ AI +EIFG+++  T+YN  +VASPIGSY
Sbjct: 438 GHLLIAFNVPGGLYVASVIIGFCFGAQWPLLFAIISEIFGLKYYSTLYNFGSVASPIGSY 497

Query: 486 VLSVRVIGYIYDREA------------SAGDNSCSGKHCFITSFLIMAAVAFLGFVVAVA 523
           +L+VRV GY+YD EA               D +C G  CF  SF+I+AAV   G +V++ 
Sbjct: 498 LLNVRVAGYLYDVEAGKQYKALGKTRVEGQDLNCIGTSCFKLSFIIIAAVTLFGVLVSMV 557