Spg021117 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg021117 vs. NCBI nr
Match: KAG6607499.1 (putative UPF0481 protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 765.4 bits (1975), Expect = 4.3e-217
Identity = 406/520 (78.08%), Postives = 450/520 (86.54%), Query Frame = 0

Query: 6   SILTGTLDKSQWVLQVNDLLDQNALPAASQTPISVFQLPETVRNGDPEAYLPRRLAVGPY 65
           SIL GTL KS WVLQ+NDL+D+NALPAAS+TPISVFQLPE+VR+GDP AYLP+ +A+GPY
Sbjct: 2   SILNGTLQKSLWVLQINDLIDRNALPAASRTPISVFQLPESVRDGDPAAYLPQFIALGPY 61

Query: 66  HHFQPELYKMDLFKLGRAKNLKWSPQIHKLADQLNPLELKIRACFDQSLDIDGETLSWVV 125
           HHF+PEL+KMDLFKLG+AKNLKW PQ+H+LADQL PLEL+IR CF  SL+IDGETLSWVV
Sbjct: 62  HHFRPELFKMDLFKLGKAKNLKWRPQLHELADQLKPLELQIRGCFCGSLEIDGETLSWVV 121

Query: 126 LIDGLFLLYLLQNKYDNRPLKLPLEIFYGKLWSEFEIVSDMVKLENQIPLFVLKEICPKE 185
           LIDGLFL++LLQN++ NRPLKLPLEIFYGKLWSEFEIVSDM+KLENQIPL VLKEICP E
Sbjct: 122 LIDGLFLIHLLQNEFSNRPLKLPLEIFYGKLWSEFEIVSDMMKLENQIPLLVLKEICPDE 181

Query: 186 PRNYLAPLLYKFYVSISPFKLPSFNI--ELPQIFDLSHHLLHFLYFLILLIPNEMVVAVI 245
           P NYLAPL Y+F  S+SPF+L  F     LP+IFD S+HLLHF+YFLILLIP+EM VA I
Sbjct: 182 PFNYLAPLFYQFSASVSPFELVCFGFASNLPEIFDSSYHLLHFVYFLILLIPDEMAVANI 241

Query: 246 AEMETGDVPISIGMDSNSSSSSSSVNFLSEFLNILGSVINIAFLQQVKETFGLIQRLFRL 305
                    + IGMDS SSSSSSSV FL E LNILGSVINIAF+QQVKETFGLIQRLFRL
Sbjct: 242 CAYS-----MPIGMDS-SSSSSSSVEFLGESLNILGSVINIAFIQQVKETFGLIQRLFRL 301

Query: 306 LGFITKPKPNVSEKK-TRSVPSASDLKTAGITFKSTENGILKCRFDETTLTLTLPSIHLD 365
           LG IT  KPN+ EKK    +PSASDLKTAGITFKST+NG+LKCRFDE TLTLTLPSIHLD
Sbjct: 302 LGSIT--KPNLCEKKLPMIIPSASDLKTAGITFKSTKNGVLKCRFDEKTLTLTLPSIHLD 361

Query: 366 GFSGVLLKNLVAFEAMAELNPPCLGVYIALMNGLLRNSKDLKILEKEEIVHNHLNSEEEA 425
           GFS VLLKNLVAFEAMAELNP CL  YIALM GLLRNSKDLKIL K EIV NHL+SEE+A
Sbjct: 362 GFSEVLLKNLVAFEAMAELNPQCLVNYIALMKGLLRNSKDLKILVKAEIVDNHLSSEEQA 421

Query: 426 VRLFNGVENSTSSLKKESVLKIHLKSGCDLEIESKFIQDIRTNYEMGLGAMVEEINKCYE 485
           V +FNGVENSTSSLKKE VL IHLKSG D EI S FIQDI+ NYEMGLG+MVEEINK Y 
Sbjct: 422 VEVFNGVENSTSSLKKEEVLNIHLKSGLDCEIGSGFIQDIKMNYEMGLGSMVEEINKWYG 481

Query: 486 SCWRMKMKKFVSVVLKCFAILVVVFLIVLVIARFICNYNS 523
           SCWRMKMKKF+SVV KCFA+LVVVF+IVLVIARF+CN+ S
Sbjct: 482 SCWRMKMKKFMSVVFKCFAVLVVVFVIVLVIARFVCNFVS 513

BLAST of Spg021117 vs. NCBI nr
Match: KAG7037156.1 (GDSL esterase/lipase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 764.2 bits (1972), Expect = 9.6e-217
Identity = 405/523 (77.44%), Postives = 450/523 (86.04%), Query Frame = 0

Query: 3   SGFSILTGTLDKSQWVLQVNDLLDQNALPAASQTPISVFQLPETVRNGDPEAYLPRRLAV 62
           S  SIL GTL KS WVLQ+NDL+D+NALPAAS+TPISVFQLPE+VR+GDP AYLP+ +A+
Sbjct: 359 SNMSILNGTLQKSLWVLQINDLIDRNALPAASRTPISVFQLPESVRDGDPAAYLPQFIAL 418

Query: 63  GPYHHFQPELYKMDLFKLGRAKNLKWSPQIHKLADQLNPLELKIRACFDQSLDIDGETLS 122
           GPYHHF+PE++KMDLFKLG+AKNLKW PQ+H+LADQL PLEL+IR CF  SL+IDGETLS
Sbjct: 419 GPYHHFRPEIFKMDLFKLGKAKNLKWRPQLHELADQLKPLELQIRGCFCGSLEIDGETLS 478

Query: 123 WVVLIDGLFLLYLLQNKYDNRPLKLPLEIFYGKLWSEFEIVSDMVKLENQIPLFVLKEIC 182
           WVVLIDGLFL++LLQN++ NRPLKLPLEIFYGKLWSEFEIVSDM+KLENQIPL VLKEIC
Sbjct: 479 WVVLIDGLFLIHLLQNEFSNRPLKLPLEIFYGKLWSEFEIVSDMMKLENQIPLLVLKEIC 538

Query: 183 PKEPRNYLAPLLYKFYVSISPFKLPSFNI--ELPQIFDLSHHLLHFLYFLILLIPNEMVV 242
           P EP NYLAPL Y+F  S+SPF+L  F     LP+IFD S+HLLHF+YFLILLIP+EM V
Sbjct: 539 PDEPFNYLAPLFYQFSASVSPFELVCFGFASNLPEIFDSSYHLLHFVYFLILLIPDEMAV 598

Query: 243 AVIAEMETGDVPISIGMDSNSSSSSSSVNFLSEFLNILGSVINIAFLQQVKETFGLIQRL 302
           A I         + IGMD  SSSSSSSV FL E LNILGSVINIAF+QQVKETFGLIQRL
Sbjct: 599 ANICAYS-----MPIGMD--SSSSSSSVEFLGESLNILGSVINIAFIQQVKETFGLIQRL 658

Query: 303 FRLLGFITKPKPNVSEKK-TRSVPSASDLKTAGITFKSTENGILKCRFDETTLTLTLPSI 362
           FRLLG IT  KPN+ EKK    +PSASDLKTAGITFKST+NG+LKCRFDE TLTLTLPSI
Sbjct: 659 FRLLGSIT--KPNLCEKKLPMIIPSASDLKTAGITFKSTKNGVLKCRFDEKTLTLTLPSI 718

Query: 363 HLDGFSGVLLKNLVAFEAMAELNPPCLGVYIALMNGLLRNSKDLKILEKEEIVHNHLNSE 422
           HLDGFS VLLKNLVAFEAMAELNP CL  YIALM GLLRNSKDLKIL K EIV NHL+SE
Sbjct: 719 HLDGFSEVLLKNLVAFEAMAELNPQCLVNYIALMKGLLRNSKDLKILVKAEIVDNHLSSE 778

Query: 423 EEAVRLFNGVENSTSSLKKESVLKIHLKSGCDLEIESKFIQDIRTNYEMGLGAMVEEINK 482
           E+AV +FNGVENSTSSLKKE VL IHLKSG D EI S FIQDI+ NYEMGLG+MVEEINK
Sbjct: 779 EQAVEVFNGVENSTSSLKKEEVLNIHLKSGLDCEIGSGFIQDIKMNYEMGLGSMVEEINK 838

Query: 483 CYESCWRMKMKKFVSVVLKCFAILVVVFLIVLVIARFICNYNS 523
            Y SCWRMKMKKF+SVV KCFA+LVVVF+IVLVIARF+CN+ S
Sbjct: 839 WYGSCWRMKMKKFMSVVFKCFAVLVVVFVIVLVIARFVCNFVS 872

BLAST of Spg021117 vs. NCBI nr
Match: XP_022949095.1 (putative UPF0481 protein At3g02645 [Cucurbita moschata])

HSP 1 Score: 761.5 bits (1965), Expect = 6.2e-216
Identity = 404/520 (77.69%), Postives = 447/520 (85.96%), Query Frame = 0

Query: 6   SILTGTLDKSQWVLQVNDLLDQNALPAASQTPISVFQLPETVRNGDPEAYLPRRLAVGPY 65
           SIL GTL KSQWVLQ+NDL+D+NALPAAS+TPISVFQLPE+VR+GDP AYLP+ +A+GPY
Sbjct: 2   SILNGTLQKSQWVLQINDLIDRNALPAASRTPISVFQLPESVRDGDPAAYLPQFIALGPY 61

Query: 66  HHFQPELYKMDLFKLGRAKNLKWSPQIHKLADQLNPLELKIRACFDQSLDIDGETLSWVV 125
           HHF+PELYKMDLFKLG+AKNLKW PQ+H+LADQL PLEL+IR CF  SL+ID ETLSWVV
Sbjct: 62  HHFRPELYKMDLFKLGKAKNLKWRPQLHELADQLKPLELQIRGCFCGSLEIDDETLSWVV 121

Query: 126 LIDGLFLLYLLQNKYDNRPLKLPLEIFYGKLWSEFEIVSDMVKLENQIPLFVLKEICPKE 185
           LIDGLFL++LLQN++ NRPLKLPLEIFYGKLWSEFEIVSDM+KLENQIPL VLKEICP E
Sbjct: 122 LIDGLFLIHLLQNEFSNRPLKLPLEIFYGKLWSEFEIVSDMMKLENQIPLLVLKEICPDE 181

Query: 186 PRNYLAPLLYKFYVSISPFKLPSFNI--ELPQIFDLSHHLLHFLYFLILLIPNEMVVAVI 245
           P NYLAPL Y+F  S+SPF+L  F     LP+IFD S+HLLHF+YFLILLIP+EM VA I
Sbjct: 182 PFNYLAPLFYQFSASVSPFELVCFGFASNLPEIFDSSYHLLHFVYFLILLIPDEMAVANI 241

Query: 246 AEMETGDVPISIGMDSNSSSSSSSVNFLSEFLNILGSVINIAFLQQVKETFGLIQRLFRL 305
                    + IGMD    SSSSSV FL E LNILGSVINIAF+QQVKETFGLIQRLFRL
Sbjct: 242 CAYS-----MPIGMD----SSSSSVEFLGESLNILGSVINIAFIQQVKETFGLIQRLFRL 301

Query: 306 LGFITKPKPNVSEKK-TRSVPSASDLKTAGITFKSTENGILKCRFDETTLTLTLPSIHLD 365
           LG IT  KPN+ EKK    +PSASDLKTAGITFKST+NG+LKCRFDE TLTLTLPSIHLD
Sbjct: 302 LGSIT--KPNLCEKKLPMIIPSASDLKTAGITFKSTKNGVLKCRFDEKTLTLTLPSIHLD 361

Query: 366 GFSGVLLKNLVAFEAMAELNPPCLGVYIALMNGLLRNSKDLKILEKEEIVHNHLNSEEEA 425
           GFS VLLKNLVAFEAMAELNP CL  YIALM GLLRNSKDLKIL K EIV NHL+SEE+A
Sbjct: 362 GFSEVLLKNLVAFEAMAELNPQCLVNYIALMKGLLRNSKDLKILVKAEIVDNHLSSEEQA 421

Query: 426 VRLFNGVENSTSSLKKESVLKIHLKSGCDLEIESKFIQDIRTNYEMGLGAMVEEINKCYE 485
           V +FNGVENSTSSLKKE VL IHLKSG D EI S FIQDI+ NYEMGLG+MVEEINK Y 
Sbjct: 422 VEVFNGVENSTSSLKKEEVLNIHLKSGLDCEIGSGFIQDIKMNYEMGLGSMVEEINKWYG 481

Query: 486 SCWRMKMKKFVSVVLKCFAILVVVFLIVLVIARFICNYNS 523
           SCWRMKMKKF+SVV KCFA+LVVVF+IVLVIARF+CN+ S
Sbjct: 482 SCWRMKMKKFMSVVFKCFAVLVVVFVIVLVIARFVCNFVS 510

BLAST of Spg021117 vs. NCBI nr
Match: XP_022973420.1 (putative UPF0481 protein At3g02645 [Cucurbita maxima])

HSP 1 Score: 753.1 bits (1943), Expect = 2.2e-213
Identity = 401/520 (77.12%), Postives = 444/520 (85.38%), Query Frame = 0

Query: 6   SILTGTLDKSQWVLQVNDLLDQNALPAASQTPISVFQLPETVRNGDPEAYLPRRLAVGPY 65
           SIL GTL+KSQWVLQ+ D++ QNALPAAS TPISVFQLPE+VR+GDP AYLP+ +A+GPY
Sbjct: 2   SILNGTLEKSQWVLQIFDIIHQNALPAASHTPISVFQLPESVRDGDPAAYLPQFIALGPY 61

Query: 66  HHFQPELYKMDLFKLGRAKNLKWSPQIHKLADQLNPLELKIRACFDQSLDIDGETLSWVV 125
           HHF+ ELYKMDLFKLG+AKNLKW PQ+H+LADQL PLEL+IR CF  SL+IDGETLSWVV
Sbjct: 62  HHFRSELYKMDLFKLGKAKNLKWRPQLHELADQLKPLELQIRGCFCGSLEIDGETLSWVV 121

Query: 126 LIDGLFLLYLLQNKYDNRPLKLPLEIFYGKLWSEFEIVSDMVKLENQIPLFVLKEICPKE 185
           LIDGLFL+ LLQN++ NRPLKLPLEIFYGKLWSEFEIVSDMVKLENQIPL VL+EICP E
Sbjct: 122 LIDGLFLIDLLQNEFSNRPLKLPLEIFYGKLWSEFEIVSDMVKLENQIPLLVLQEICPDE 181

Query: 186 PRNYLAPLLYKFYVSISPFKLPSFNI--ELPQIFDLSHHLLHFLYFLILLIPNEMVVAVI 245
           P NYLAPL Y+F  S+SPF+L  F     LP+IFD SHHLLHF+YFLILLIP+EM VA I
Sbjct: 182 PFNYLAPLFYQFSASVSPFELVCFGFASNLPEIFDSSHHLLHFVYFLILLIPDEMAVANI 241

Query: 246 AEMETGDVPISIGMDSNSSSSSSSVNFLSEFLNILGSVINIAFLQQVKETFGLIQRLFRL 305
                    + IGMD   SSSSSSV  L E LNILGSVI+IAF+QQVKETFGLI+RLFRL
Sbjct: 242 CAYS-----MPIGMD---SSSSSSVELLGESLNILGSVISIAFIQQVKETFGLIRRLFRL 301

Query: 306 LGFITKPKPNVSEKK-TRSVPSASDLKTAGITFKSTENGILKCRFDETTLTLTLPSIHLD 365
           LG IT  KPN+ EKK    +PSASDLKTAGITFKST NG+LKCRFDE TLTLTLPSIHLD
Sbjct: 302 LGSIT--KPNLCEKKLPMIIPSASDLKTAGITFKSTNNGVLKCRFDEKTLTLTLPSIHLD 361

Query: 366 GFSGVLLKNLVAFEAMAELNPPCLGVYIALMNGLLRNSKDLKILEKEEIVHNHLNSEEEA 425
            FS VLLKNLVAFEAMAELNP CL  YIALM GLLRNSKDLKILEK EIVHNHL++EE+A
Sbjct: 362 WFSEVLLKNLVAFEAMAELNPQCLVNYIALMKGLLRNSKDLKILEKAEIVHNHLSNEEQA 421

Query: 426 VRLFNGVENSTSSLKKESVLKIHLKSGCDLEIESKFIQDIRTNYEMGLGAMVEEINKCYE 485
           V +FNGVENSTSSLKKE VLKIHLKSG D EI S FIQDI+ NYEMGLG+MVEEINK Y 
Sbjct: 422 VEVFNGVENSTSSLKKEEVLKIHLKSGLDCEIGSGFIQDIKMNYEMGLGSMVEEINKWYG 481

Query: 486 SCWRMKMKKFVSVVLKCFAILVVVFLIVLVIARFICNYNS 523
           SCWRMKMK+F+SVV KCFA+LVVVF+IVLVIARF CN+ S
Sbjct: 482 SCWRMKMKRFMSVVFKCFAVLVVVFVIVLVIARFACNFVS 511

BLAST of Spg021117 vs. NCBI nr
Match: KAA0039249.1 (putative UPF0481 protein [Cucumis melo var. makuwa] >TYK00435.1 putative UPF0481 protein [Cucumis melo var. makuwa])

HSP 1 Score: 733.4 bits (1892), Expect = 1.8e-207
Identity = 387/532 (72.74%), Postives = 437/532 (82.14%), Query Frame = 0

Query: 1   MASGFSILTGTLDKSQWVLQVNDLLDQNALPAASQTPISVFQLPETVRNGDPEAYLPRRL 60
           MASGFSILTGT DKSQWVLQ+NDL+DQNAL  A+QTPIS+F+LPE+V +GDP AYLPR +
Sbjct: 1   MASGFSILTGTFDKSQWVLQINDLVDQNALLDATQTPISIFRLPESVHDGDPTAYLPRHV 60

Query: 61  AVGPYHHFQPELYKMDLFKLGRAKNLKWSPQIHKLADQLNPLELKIRACFDQSLDIDGET 120
           A GP+HHFQPELYKM+LFKLG+AKNLKW PQIHKLAD+L PLELK+RACFDQ+L+I+GET
Sbjct: 61  AFGPFHHFQPELYKMELFKLGKAKNLKWGPQIHKLADRLTPLELKMRACFDQALEINGET 120

Query: 121 LSWVVLIDGLFLLYLLQNKYDNRPLKLPLEIFYGKLWSEFEIVSDMVKLENQIPLFVLKE 180
           LSWV+LIDGLFL+YLLQNKY NRPL+ P EIFYGKLWSEFEIV DMVKLENQIPLFVL E
Sbjct: 121 LSWVLLIDGLFLIYLLQNKYMNRPLRFPSEIFYGKLWSEFEIVGDMVKLENQIPLFVLNE 180

Query: 181 ICPKEPRNYLAPLLYKFYVSISPFKLPSFNIE---------LPQIFDLSHHLLHFLYFLI 240
           ICPKEP N+LAP LY+F VS+S F LP FN +         LP+IFDLSHHLLHFLY LI
Sbjct: 181 ICPKEPINFLAPFLYQFCVSVSSFPLPRFNPQLECFGFSSYLPEIFDLSHHLLHFLYSLI 240

Query: 241 LLIPNEMVVAVIAEMETGDVPISIGMDSNSSSSSSSVNFLSEFLNILGSVINIAFLQQVK 300
           LLIP+E V           +P    +D   +SSSSSV FLSE L+ILGSVINIAF+QQ+K
Sbjct: 241 LLIPDERVAF---------MPYCFRLD---NSSSSSVEFLSESLDILGSVINIAFIQQIK 300

Query: 301 ETFGLIQRLFRLLGFITKPKPNVSEK-KTRSVPSASDLKTAGITFKSTENGILKCRFDET 360
           ET GLIQRL RLL  IT  KPN++EK     +PSASDLK+AG TFKST+NGILK  FDET
Sbjct: 301 ETIGLIQRLLRLLSSIT--KPNLAEKTPPLIIPSASDLKSAGFTFKSTKNGILKSYFDET 360

Query: 361 TLTLTLPSIHLDGFSGVLLKNLVAFEAMAELNPPCLGVYIALMNGLLRNSKDLKILEKEE 420
           TLTLTLP IHL GF+ VLLKNLVAFE+MAELNPPCL  Y ALMNGLLR SKDLK+LEK E
Sbjct: 361 TLTLTLPCIHLHGFTHVLLKNLVAFESMAELNPPCLANYTALMNGLLRKSKDLKVLEKAE 420

Query: 421 IVHNHLNSEEEAVRLFNGVENSTSSLKKESVLKIHLKSGCDLEIESKFIQDIRTNYEMGL 480
           IVHNHLNSEEEA  LF GVENSTS LKKES+L+IHLK G + E  S FI ++ ++YEMGL
Sbjct: 421 IVHNHLNSEEEAAELFYGVENSTSRLKKESLLEIHLKGGFNFENGSNFIPEVESSYEMGL 480

Query: 481 GAMVEEINKCYESCWRMKMKKFVSVVLKCFAILVVVFLIVLVIARFICNYNS 523
           G MVE IN CY +CWRMKMKK VSVV KCFA+LVVVFLIVLV  RF+CN+ S
Sbjct: 481 GNMVERINNCYGNCWRMKMKKVVSVVYKCFAVLVVVFLIVLVTTRFVCNFMS 518

BLAST of Spg021117 vs. ExPASy Swiss-Prot
Match: P0C897 (Putative UPF0481 protein At3g02645 OS=Arabidopsis thaliana OX=3702 GN=At3g02645 PE=3 SV=1)

HSP 1 Score: 157.5 bits (397), Expect = 5.4e-37
Identity = 145/545 (26.61%), Postives = 254/545 (46.61%), Query Frame = 0

Query: 13  DKSQWVLQVNDLLDQNALP-AASQTPISVFQLPETVRNGDPEAYLPRRLAVGPYHHFQPE 72
           D+++WV+ V   LD         +  +S+F +P+ +    P++Y P R+++GPYH  +PE
Sbjct: 17  DETRWVINVQKSLDAELEEHDLEEVTVSIFNVPKALMCSHPDSYTPHRVSIGPYHCLKPE 76

Query: 73  LYKMDLFKLGRAKNLK---WSPQIHKLADQLNPLELKIRACFDQSLDIDGETLSWVVLID 132
           L++M+ +KL  A+ ++    S + H L ++L  +E+KIRAC+ + +  +GETL W++ +D
Sbjct: 77  LHEMERYKLMIARKIRNQYNSFRFHDLVEKLQSMEIKIRACYHKYIGFNGETLLWIMAVD 136

Query: 133 GLFLLYLLQNKYDNRPLKLPLEIFYGKLWSEFEIVSDMVKLENQIPLFVLKEI------C 192
             FL+  L+  Y  R     +E    ++    EI+ D++ +ENQIPLFVL++        
Sbjct: 137 SSFLIEFLK-IYSFR----KVETLINRV-GHNEILRDIMMIENQIPLFVLRKTLEFQLES 196

Query: 193 PKEPRNYLAPLLYKFYVSISPFKLPSFNIE-LPQIFDLSHHLLHFLYFLILLIPNEMVVA 252
            +   + L  +L      +SP  +   + + L   F   +H+L FLY        +M+V 
Sbjct: 197 TESADDLLLSVLTGLCKDLSPLVIKFDDDQILKAQFQECNHILDFLY--------QMIVP 256

Query: 253 VIAEMETGDVPISIGMDSNSSSSSSSVNFLSE----FLNILGS-------------VINI 312
            I E E  +       D N    + ++ F+ E    F  +  S             + N+
Sbjct: 257 RIEEEELEEDDEENRADEN--GGNRAIRFMDEIKHQFKRVFASRPADLILRFPWRIISNL 316

Query: 313 ------------AFLQQVKETFGLIQRLFRLLGFITKPKPNVSEKKTRSVPSASDLKTAG 372
                        F +Q  E     Q    +L      KP + E+ T  +PS SDL  AG
Sbjct: 317 PGFMALKLSADYLFTRQENEATTTRQESVSILDI---EKPPLVEELT--IPSVSDLHKAG 376

Query: 373 ITFKSTENG-ILKCRFDETTLTLTLPSIHLDGFSGVLLKNLVAFEAMAELNPPCLGVYIA 432
           + FK T +G I    FD  +    LP I+LD  +  +L+NLVA+EA     P     Y  
Sbjct: 377 VRFKPTAHGNISTVTFDSNSGQFYLPVINLDINTETVLRNLVAYEATNTSGPLVFTRYTE 436

Query: 433 LMNGLLRNSKDLKILEKEEIVHNHLNSEEEAVRLFNGVENSTSSLKKESVLKIHLKSGCD 492
           L+NG++ + +D+++L ++ ++ + L S++EA  ++NG+  S                   
Sbjct: 437 LINGIIDSEEDVRLLREQGVLVSRLKSDQEAAEMWNGMSKS------------------- 496

Query: 493 LEIESKFIQDIRTNYEMGLGAMVEEINKCYESCWRMKMKKFVSVVL----KCFAILVVVF 513
                     +R      L   +E++N+ Y   W++K+ + V V +    +  A L  V 
Sbjct: 497 ----------VRLTKVGFLDKTIEDVNRYYTGRWKVKIGRLVEVYVYGSWQILAFLAAVL 511

BLAST of Spg021117 vs. ExPASy Swiss-Prot
Match: Q9SD53 (UPF0481 protein At3g47200 OS=Arabidopsis thaliana OX=3702 GN=At3g47200 PE=2 SV=1)

HSP 1 Score: 77.8 bits (190), Expect = 5.4e-13
Identity = 100/411 (24.33%), Postives = 164/411 (39.90%), Query Frame = 0

Query: 30  LPAASQTPISVFQLPETVRNGDPEAYLPRRLAVGPYHHFQPEL-----YKMDLFKLGRAK 89
           L +A +    +F++PE+    +P+AY P+ +++GPYH+ +  L     +K  L +L   +
Sbjct: 38  LESAGKESCCIFRVPESFVALNPKAYKPKVVSIGPYHYGEKHLQMIQQHKPRLLQLFLDE 97

Query: 90  NLKWSPQIHKLADQLNPLELKIRACFDQSLDIDGETLSWVVLIDGLFLLYLLQNKYDNRP 149
             K   + + L   +  LE KIR  + + L   G  L +++++DG F+L +      N  
Sbjct: 98  AKKKDVEENVLVKAVVDLEDKIRKSYSEELK-TGHDLMFMMVLDGCFILMVFLIMSGN-- 157

Query: 150 LKLPLEIFYGKLWSEFEIVSDMVKLENQIPLFVLKEICPKEPRNYLAPLLYKFYVSISPF 209
           ++L  +  +   W    I SD++ LENQ+P FVL+ +                YV  S  
Sbjct: 158 IELSEDPIFSIPWLLSSIQSDLLLLENQVPFFVLQTL----------------YVG-SKI 217

Query: 210 KLPSFNIELPQIFDLSHHLLHFLYFLILLIPNEMVVAVIAEMETGDVPISIGMDSNSSSS 269
            + S         DL+    HF                              +D   S  
Sbjct: 218 GVSS---------DLNRIAFHF--------------------------FKNPIDKEGSYW 277

Query: 270 SSSVNFLSEFLNILGSVINIAFLQQVKETFGLIQRLFRLLGFITKP--KPNVSEKKTRSV 329
               N+ ++ L     +I   FL    E+        ++     K    P+V  K    +
Sbjct: 278 EKHRNYKAKHLL---DLIRETFLPNTSESDKASSPHVQVQLHEGKSGNVPSVDSKAVPLI 337

Query: 330 PSASDLKTAGITF---KSTENGILKCRFDETTLTLTLPSIHLDGFSGVLLKNLVAFEAMA 389
            SA  L+  GI F   +S E+ IL  R  +    L +P +  DGF      N VAFE   
Sbjct: 338 LSAKRLRLQGIKFRLRRSKEDSILNVRLKKN--KLQIPQLRFDGFISSFFLNCVAFEQFY 388

Query: 390 ELNPPCLGVYIALMNGLLRNSKDLKILEKEE-IVHNHLNSEEEAVRLFNGV 430
             +   +  YI  M  LL N +D+  L  ++ I+ NH  S  E    F  +
Sbjct: 398 TDSSNEITTYIVFMGCLLNNEEDVTFLRNDKLIIENHFGSNNEVSEFFKTI 388

BLAST of Spg021117 vs. ExPASy TrEMBL
Match: A0A6J1GBT5 (putative UPF0481 protein At3g02645 OS=Cucurbita moschata OX=3662 GN=LOC111452550 PE=4 SV=1)

HSP 1 Score: 761.5 bits (1965), Expect = 3.0e-216
Identity = 404/520 (77.69%), Postives = 447/520 (85.96%), Query Frame = 0

Query: 6   SILTGTLDKSQWVLQVNDLLDQNALPAASQTPISVFQLPETVRNGDPEAYLPRRLAVGPY 65
           SIL GTL KSQWVLQ+NDL+D+NALPAAS+TPISVFQLPE+VR+GDP AYLP+ +A+GPY
Sbjct: 2   SILNGTLQKSQWVLQINDLIDRNALPAASRTPISVFQLPESVRDGDPAAYLPQFIALGPY 61

Query: 66  HHFQPELYKMDLFKLGRAKNLKWSPQIHKLADQLNPLELKIRACFDQSLDIDGETLSWVV 125
           HHF+PELYKMDLFKLG+AKNLKW PQ+H+LADQL PLEL+IR CF  SL+ID ETLSWVV
Sbjct: 62  HHFRPELYKMDLFKLGKAKNLKWRPQLHELADQLKPLELQIRGCFCGSLEIDDETLSWVV 121

Query: 126 LIDGLFLLYLLQNKYDNRPLKLPLEIFYGKLWSEFEIVSDMVKLENQIPLFVLKEICPKE 185
           LIDGLFL++LLQN++ NRPLKLPLEIFYGKLWSEFEIVSDM+KLENQIPL VLKEICP E
Sbjct: 122 LIDGLFLIHLLQNEFSNRPLKLPLEIFYGKLWSEFEIVSDMMKLENQIPLLVLKEICPDE 181

Query: 186 PRNYLAPLLYKFYVSISPFKLPSFNI--ELPQIFDLSHHLLHFLYFLILLIPNEMVVAVI 245
           P NYLAPL Y+F  S+SPF+L  F     LP+IFD S+HLLHF+YFLILLIP+EM VA I
Sbjct: 182 PFNYLAPLFYQFSASVSPFELVCFGFASNLPEIFDSSYHLLHFVYFLILLIPDEMAVANI 241

Query: 246 AEMETGDVPISIGMDSNSSSSSSSVNFLSEFLNILGSVINIAFLQQVKETFGLIQRLFRL 305
                    + IGMD    SSSSSV FL E LNILGSVINIAF+QQVKETFGLIQRLFRL
Sbjct: 242 CAYS-----MPIGMD----SSSSSVEFLGESLNILGSVINIAFIQQVKETFGLIQRLFRL 301

Query: 306 LGFITKPKPNVSEKK-TRSVPSASDLKTAGITFKSTENGILKCRFDETTLTLTLPSIHLD 365
           LG IT  KPN+ EKK    +PSASDLKTAGITFKST+NG+LKCRFDE TLTLTLPSIHLD
Sbjct: 302 LGSIT--KPNLCEKKLPMIIPSASDLKTAGITFKSTKNGVLKCRFDEKTLTLTLPSIHLD 361

Query: 366 GFSGVLLKNLVAFEAMAELNPPCLGVYIALMNGLLRNSKDLKILEKEEIVHNHLNSEEEA 425
           GFS VLLKNLVAFEAMAELNP CL  YIALM GLLRNSKDLKIL K EIV NHL+SEE+A
Sbjct: 362 GFSEVLLKNLVAFEAMAELNPQCLVNYIALMKGLLRNSKDLKILVKAEIVDNHLSSEEQA 421

Query: 426 VRLFNGVENSTSSLKKESVLKIHLKSGCDLEIESKFIQDIRTNYEMGLGAMVEEINKCYE 485
           V +FNGVENSTSSLKKE VL IHLKSG D EI S FIQDI+ NYEMGLG+MVEEINK Y 
Sbjct: 422 VEVFNGVENSTSSLKKEEVLNIHLKSGLDCEIGSGFIQDIKMNYEMGLGSMVEEINKWYG 481

Query: 486 SCWRMKMKKFVSVVLKCFAILVVVFLIVLVIARFICNYNS 523
           SCWRMKMKKF+SVV KCFA+LVVVF+IVLVIARF+CN+ S
Sbjct: 482 SCWRMKMKKFMSVVFKCFAVLVVVFVIVLVIARFVCNFVS 510

BLAST of Spg021117 vs. ExPASy TrEMBL
Match: A0A6J1IBB2 (putative UPF0481 protein At3g02645 OS=Cucurbita maxima OX=3661 GN=LOC111471968 PE=4 SV=1)

HSP 1 Score: 753.1 bits (1943), Expect = 1.1e-213
Identity = 401/520 (77.12%), Postives = 444/520 (85.38%), Query Frame = 0

Query: 6   SILTGTLDKSQWVLQVNDLLDQNALPAASQTPISVFQLPETVRNGDPEAYLPRRLAVGPY 65
           SIL GTL+KSQWVLQ+ D++ QNALPAAS TPISVFQLPE+VR+GDP AYLP+ +A+GPY
Sbjct: 2   SILNGTLEKSQWVLQIFDIIHQNALPAASHTPISVFQLPESVRDGDPAAYLPQFIALGPY 61

Query: 66  HHFQPELYKMDLFKLGRAKNLKWSPQIHKLADQLNPLELKIRACFDQSLDIDGETLSWVV 125
           HHF+ ELYKMDLFKLG+AKNLKW PQ+H+LADQL PLEL+IR CF  SL+IDGETLSWVV
Sbjct: 62  HHFRSELYKMDLFKLGKAKNLKWRPQLHELADQLKPLELQIRGCFCGSLEIDGETLSWVV 121

Query: 126 LIDGLFLLYLLQNKYDNRPLKLPLEIFYGKLWSEFEIVSDMVKLENQIPLFVLKEICPKE 185
           LIDGLFL+ LLQN++ NRPLKLPLEIFYGKLWSEFEIVSDMVKLENQIPL VL+EICP E
Sbjct: 122 LIDGLFLIDLLQNEFSNRPLKLPLEIFYGKLWSEFEIVSDMVKLENQIPLLVLQEICPDE 181

Query: 186 PRNYLAPLLYKFYVSISPFKLPSFNI--ELPQIFDLSHHLLHFLYFLILLIPNEMVVAVI 245
           P NYLAPL Y+F  S+SPF+L  F     LP+IFD SHHLLHF+YFLILLIP+EM VA I
Sbjct: 182 PFNYLAPLFYQFSASVSPFELVCFGFASNLPEIFDSSHHLLHFVYFLILLIPDEMAVANI 241

Query: 246 AEMETGDVPISIGMDSNSSSSSSSVNFLSEFLNILGSVINIAFLQQVKETFGLIQRLFRL 305
                    + IGMD   SSSSSSV  L E LNILGSVI+IAF+QQVKETFGLI+RLFRL
Sbjct: 242 CAYS-----MPIGMD---SSSSSSVELLGESLNILGSVISIAFIQQVKETFGLIRRLFRL 301

Query: 306 LGFITKPKPNVSEKK-TRSVPSASDLKTAGITFKSTENGILKCRFDETTLTLTLPSIHLD 365
           LG IT  KPN+ EKK    +PSASDLKTAGITFKST NG+LKCRFDE TLTLTLPSIHLD
Sbjct: 302 LGSIT--KPNLCEKKLPMIIPSASDLKTAGITFKSTNNGVLKCRFDEKTLTLTLPSIHLD 361

Query: 366 GFSGVLLKNLVAFEAMAELNPPCLGVYIALMNGLLRNSKDLKILEKEEIVHNHLNSEEEA 425
            FS VLLKNLVAFEAMAELNP CL  YIALM GLLRNSKDLKILEK EIVHNHL++EE+A
Sbjct: 362 WFSEVLLKNLVAFEAMAELNPQCLVNYIALMKGLLRNSKDLKILEKAEIVHNHLSNEEQA 421

Query: 426 VRLFNGVENSTSSLKKESVLKIHLKSGCDLEIESKFIQDIRTNYEMGLGAMVEEINKCYE 485
           V +FNGVENSTSSLKKE VLKIHLKSG D EI S FIQDI+ NYEMGLG+MVEEINK Y 
Sbjct: 422 VEVFNGVENSTSSLKKEEVLKIHLKSGLDCEIGSGFIQDIKMNYEMGLGSMVEEINKWYG 481

Query: 486 SCWRMKMKKFVSVVLKCFAILVVVFLIVLVIARFICNYNS 523
           SCWRMKMK+F+SVV KCFA+LVVVF+IVLVIARF CN+ S
Sbjct: 482 SCWRMKMKRFMSVVFKCFAVLVVVFVIVLVIARFACNFVS 511

BLAST of Spg021117 vs. ExPASy TrEMBL
Match: A0A5D3BMY1 (Putative UPF0481 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G00380 PE=4 SV=1)

HSP 1 Score: 733.4 bits (1892), Expect = 8.8e-208
Identity = 387/532 (72.74%), Postives = 437/532 (82.14%), Query Frame = 0

Query: 1   MASGFSILTGTLDKSQWVLQVNDLLDQNALPAASQTPISVFQLPETVRNGDPEAYLPRRL 60
           MASGFSILTGT DKSQWVLQ+NDL+DQNAL  A+QTPIS+F+LPE+V +GDP AYLPR +
Sbjct: 1   MASGFSILTGTFDKSQWVLQINDLVDQNALLDATQTPISIFRLPESVHDGDPTAYLPRHV 60

Query: 61  AVGPYHHFQPELYKMDLFKLGRAKNLKWSPQIHKLADQLNPLELKIRACFDQSLDIDGET 120
           A GP+HHFQPELYKM+LFKLG+AKNLKW PQIHKLAD+L PLELK+RACFDQ+L+I+GET
Sbjct: 61  AFGPFHHFQPELYKMELFKLGKAKNLKWGPQIHKLADRLTPLELKMRACFDQALEINGET 120

Query: 121 LSWVVLIDGLFLLYLLQNKYDNRPLKLPLEIFYGKLWSEFEIVSDMVKLENQIPLFVLKE 180
           LSWV+LIDGLFL+YLLQNKY NRPL+ P EIFYGKLWSEFEIV DMVKLENQIPLFVL E
Sbjct: 121 LSWVLLIDGLFLIYLLQNKYMNRPLRFPSEIFYGKLWSEFEIVGDMVKLENQIPLFVLNE 180

Query: 181 ICPKEPRNYLAPLLYKFYVSISPFKLPSFNIE---------LPQIFDLSHHLLHFLYFLI 240
           ICPKEP N+LAP LY+F VS+S F LP FN +         LP+IFDLSHHLLHFLY LI
Sbjct: 181 ICPKEPINFLAPFLYQFCVSVSSFPLPRFNPQLECFGFSSYLPEIFDLSHHLLHFLYSLI 240

Query: 241 LLIPNEMVVAVIAEMETGDVPISIGMDSNSSSSSSSVNFLSEFLNILGSVINIAFLQQVK 300
           LLIP+E V           +P    +D   +SSSSSV FLSE L+ILGSVINIAF+QQ+K
Sbjct: 241 LLIPDERVAF---------MPYCFRLD---NSSSSSVEFLSESLDILGSVINIAFIQQIK 300

Query: 301 ETFGLIQRLFRLLGFITKPKPNVSEK-KTRSVPSASDLKTAGITFKSTENGILKCRFDET 360
           ET GLIQRL RLL  IT  KPN++EK     +PSASDLK+AG TFKST+NGILK  FDET
Sbjct: 301 ETIGLIQRLLRLLSSIT--KPNLAEKTPPLIIPSASDLKSAGFTFKSTKNGILKSYFDET 360

Query: 361 TLTLTLPSIHLDGFSGVLLKNLVAFEAMAELNPPCLGVYIALMNGLLRNSKDLKILEKEE 420
           TLTLTLP IHL GF+ VLLKNLVAFE+MAELNPPCL  Y ALMNGLLR SKDLK+LEK E
Sbjct: 361 TLTLTLPCIHLHGFTHVLLKNLVAFESMAELNPPCLANYTALMNGLLRKSKDLKVLEKAE 420

Query: 421 IVHNHLNSEEEAVRLFNGVENSTSSLKKESVLKIHLKSGCDLEIESKFIQDIRTNYEMGL 480
           IVHNHLNSEEEA  LF GVENSTS LKKES+L+IHLK G + E  S FI ++ ++YEMGL
Sbjct: 421 IVHNHLNSEEEAAELFYGVENSTSRLKKESLLEIHLKGGFNFENGSNFIPEVESSYEMGL 480

Query: 481 GAMVEEINKCYESCWRMKMKKFVSVVLKCFAILVVVFLIVLVIARFICNYNS 523
           G MVE IN CY +CWRMKMKK VSVV KCFA+LVVVFLIVLV  RF+CN+ S
Sbjct: 481 GNMVERINNCYGNCWRMKMKKVVSVVYKCFAVLVVVFLIVLVTTRFVCNFMS 518

BLAST of Spg021117 vs. ExPASy TrEMBL
Match: A0A0A0KSX9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G650580 PE=4 SV=1)

HSP 1 Score: 733.4 bits (1892), Expect = 8.8e-208
Identity = 393/533 (73.73%), Postives = 441/533 (82.74%), Query Frame = 0

Query: 1   MASGFSILTGTLDKSQWVLQVNDLLDQNALPAASQTPISVFQLPETVRNGDPEAYLPRRL 60
           MASGFSILTGT DKSQW+ Q+N+L++Q+AL AASQ PISVF+LPE+V + DP AYLPR +
Sbjct: 1   MASGFSILTGTFDKSQWLHQINNLVNQDALLAASQIPISVFRLPESVHDVDPTAYLPRHV 60

Query: 61  AVGPYHHFQPELYKMDLFKLGRAKNLKWSPQIHKLADQLNPLELKIRACFDQSLDIDGET 120
           A GP+HHFQPELYKM+LFKLG+AKNLKW PQIHKL+D+L PLELKIRACFDQ+L+I+GET
Sbjct: 61  AFGPFHHFQPELYKMELFKLGKAKNLKWGPQIHKLSDRLTPLELKIRACFDQALEINGET 120

Query: 121 LSWVVLIDGLFLLYLLQNKYDNRPLKLPLEIFYGKLWSEFEIVSDMVKLENQIPLFVLKE 180
           LSW++LIDGLFL+YLLQN+Y N PL  P EIFYGKLWSEFEIV DMVKLENQIPLFVL E
Sbjct: 121 LSWLLLIDGLFLIYLLQNEYMNCPLTFPSEIFYGKLWSEFEIVCDMVKLENQIPLFVLNE 180

Query: 181 ICPKEPRNYLAPLLYKFYVSISPFKLPSFNIE---------LPQIFDLSHHLLHFLYFLI 240
           ICP+EP N+LAP LY+F VS+SPF+LP FN           LP+IFDLSHHLLHFLY LI
Sbjct: 181 ICPEEPINFLAPSLYQFCVSVSPFQLPCFNPRLECFGFSSYLPEIFDLSHHLLHFLYSLI 240

Query: 241 LLIPNEMVVAVIAEMETGDVPISIGMDSNSSSSSSSVNFLSEFLNILGSVINIAFLQQVK 300
           LLIPNE VV V              MD NSSSSSSSV FLSE L+ILGSVINIAF+QQ+K
Sbjct: 241 LLIPNERVVFVAH---------CFRMD-NSSSSSSSVEFLSECLDILGSVINIAFIQQIK 300

Query: 301 ETFGLIQRLFRLLGFITKPKPNVSEK-KTRSVPSASDLKTAGITFKSTENGILKCRFDET 360
           ET GLIQRL RLL FIT  KPN++EK     +PSASDLK+AG TFKST+NGILK  FDET
Sbjct: 301 ETIGLIQRLLRLLSFIT--KPNLAEKTPLLIIPSASDLKSAGFTFKSTKNGILKSNFDET 360

Query: 361 TLTLTLPSIHLDGFSGVLLKNLVAFEAMAELNPPCLGVYIALMNGLLRNSKDLKILEKEE 420
           TLTLTLP IHLDGF+ VLLKNLVAFEAMAELNPPCL  Y ALMNGLLRNSKDLK+LEK E
Sbjct: 361 TLTLTLPCIHLDGFTHVLLKNLVAFEAMAELNPPCLANYTALMNGLLRNSKDLKVLEKAE 420

Query: 421 IVHNHLNSEEEAVRLFNGVENSTSSLKKESVLKIHLKSGCDLEIESKFIQDIRTNY-EMG 480
           IVHNHLNSEEEA  LF GVENSTS LKK S+LKIHL SG + E  SKFIQ++ ++Y EMG
Sbjct: 421 IVHNHLNSEEEAAELFYGVENSTSKLKKGSLLKIHLNSGFNFENGSKFIQEVESSYIEMG 480

Query: 481 LGAMVEEINKCYESCWRMKMKKFVSVVLKCFAILVVVFLIVLVIARFICNYNS 523
           LG MVE IN CY +CWRMKMKKFVSVV KCFA+LVVVFLIVLV  RF+CN+ S
Sbjct: 481 LGDMVERINNCYGNCWRMKMKKFVSVVYKCFAVLVVVFLIVLVTTRFVCNFLS 521

BLAST of Spg021117 vs. ExPASy TrEMBL
Match: A0A1S3CAN6 (putative UPF0481 protein At3g02645 OS=Cucumis melo OX=3656 GN=LOC103498704 PE=4 SV=1)

HSP 1 Score: 731.1 bits (1886), Expect = 4.3e-207
Identity = 384/532 (72.18%), Postives = 437/532 (82.14%), Query Frame = 0

Query: 1   MASGFSILTGTLDKSQWVLQVNDLLDQNALPAASQTPISVFQLPETVRNGDPEAYLPRRL 60
           MASGFSILTGT DKSQWVLQ+N+L+DQNAL  A+QTPISVF+LPE+V +GDP AYLPR +
Sbjct: 1   MASGFSILTGTFDKSQWVLQINNLVDQNALLDATQTPISVFRLPESVHDGDPTAYLPRHV 60

Query: 61  AVGPYHHFQPELYKMDLFKLGRAKNLKWSPQIHKLADQLNPLELKIRACFDQSLDIDGET 120
           A GP+HHFQPEL+KM+LFKLG+AKNLKW PQIHKLAD+L PLELK+RACFDQ+L+I+GET
Sbjct: 61  AFGPFHHFQPELHKMELFKLGKAKNLKWGPQIHKLADRLTPLELKMRACFDQALEINGET 120

Query: 121 LSWVVLIDGLFLLYLLQNKYDNRPLKLPLEIFYGKLWSEFEIVSDMVKLENQIPLFVLKE 180
           LSWV+LIDGLFL+YLLQNKY N PL+ P EIFYGKLWSEFEIV DMVKLENQIPLFVL E
Sbjct: 121 LSWVLLIDGLFLIYLLQNKYMNCPLRFPSEIFYGKLWSEFEIVCDMVKLENQIPLFVLNE 180

Query: 181 ICPKEPRNYLAPLLYKFYVSISPFKLPSFNIE---------LPQIFDLSHHLLHFLYFLI 240
           ICPKEP N+LAP LY+F VS+SPF LP FN +         LP+IFDLSHHLLHFLY LI
Sbjct: 181 ICPKEPINFLAPFLYQFCVSVSPFPLPRFNPQLECFGFSSYLPEIFDLSHHLLHFLYSLI 240

Query: 241 LLIPNEMVVAVIAEMETGDVPISIGMDSNSSSSSSSVNFLSEFLNILGSVINIAFLQQVK 300
           LLIP+E VV ++  +               +SSSSSV FLSE L+ILGSVINIAF+QQ+K
Sbjct: 241 LLIPDERVVIMLFGLRL------------DNSSSSSVEFLSESLDILGSVINIAFIQQIK 300

Query: 301 ETFGLIQRLFRLLGFITKPKPNVSEK-KTRSVPSASDLKTAGITFKSTENGILKCRFDET 360
           ET GLIQRL RLL  IT  KPN++EK     +PSAS LK+AG TFKST+NGILK  FDET
Sbjct: 301 ETIGLIQRLLRLLSSIT--KPNLAEKTPPLIIPSASHLKSAGFTFKSTKNGILKSYFDET 360

Query: 361 TLTLTLPSIHLDGFSGVLLKNLVAFEAMAELNPPCLGVYIALMNGLLRNSKDLKILEKEE 420
           TLTLTLP IHL GF+ VLLKNLVAFE+MAELNPPCL  Y ALMNGLLRNSKDLK+LEK E
Sbjct: 361 TLTLTLPCIHLHGFTHVLLKNLVAFESMAELNPPCLANYTALMNGLLRNSKDLKVLEKAE 420

Query: 421 IVHNHLNSEEEAVRLFNGVENSTSSLKKESVLKIHLKSGCDLEIESKFIQDIRTNYEMGL 480
           IVHNHLNSEEEA  LF GVENST  LKKES+L+IHLK G + E  S FIQ++ ++YEMGL
Sbjct: 421 IVHNHLNSEEEAAELFYGVENSTRRLKKESLLEIHLKGGFNFENGSNFIQEVESSYEMGL 480

Query: 481 GAMVEEINKCYESCWRMKMKKFVSVVLKCFAILVVVFLIVLVIARFICNYNS 523
           G MVE IN CY +CWRMKMKK VSVV KCFA+L+VVFLIVLV  RF+CN+ S
Sbjct: 481 GNMVERINNCYGNCWRMKMKKVVSVVFKCFAVLIVVFLIVLVTTRFVCNFMS 518

BLAST of Spg021117 vs. TAIR 10
Match: AT3G02645.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 157.5 bits (397), Expect = 3.8e-38
Identity = 145/545 (26.61%), Postives = 254/545 (46.61%), Query Frame = 0

Query: 13  DKSQWVLQVNDLLDQNALP-AASQTPISVFQLPETVRNGDPEAYLPRRLAVGPYHHFQPE 72
           D+++WV+ V   LD         +  +S+F +P+ +    P++Y P R+++GPYH  +PE
Sbjct: 17  DETRWVINVQKSLDAELEEHDLEEVTVSIFNVPKALMCSHPDSYTPHRVSIGPYHCLKPE 76

Query: 73  LYKMDLFKLGRAKNLK---WSPQIHKLADQLNPLELKIRACFDQSLDIDGETLSWVVLID 132
           L++M+ +KL  A+ ++    S + H L ++L  +E+KIRAC+ + +  +GETL W++ +D
Sbjct: 77  LHEMERYKLMIARKIRNQYNSFRFHDLVEKLQSMEIKIRACYHKYIGFNGETLLWIMAVD 136

Query: 133 GLFLLYLLQNKYDNRPLKLPLEIFYGKLWSEFEIVSDMVKLENQIPLFVLKEI------C 192
             FL+  L+  Y  R     +E    ++    EI+ D++ +ENQIPLFVL++        
Sbjct: 137 SSFLIEFLK-IYSFR----KVETLINRV-GHNEILRDIMMIENQIPLFVLRKTLEFQLES 196

Query: 193 PKEPRNYLAPLLYKFYVSISPFKLPSFNIE-LPQIFDLSHHLLHFLYFLILLIPNEMVVA 252
            +   + L  +L      +SP  +   + + L   F   +H+L FLY        +M+V 
Sbjct: 197 TESADDLLLSVLTGLCKDLSPLVIKFDDDQILKAQFQECNHILDFLY--------QMIVP 256

Query: 253 VIAEMETGDVPISIGMDSNSSSSSSSVNFLSE----FLNILGS-------------VINI 312
            I E E  +       D N    + ++ F+ E    F  +  S             + N+
Sbjct: 257 RIEEEELEEDDEENRADEN--GGNRAIRFMDEIKHQFKRVFASRPADLILRFPWRIISNL 316

Query: 313 ------------AFLQQVKETFGLIQRLFRLLGFITKPKPNVSEKKTRSVPSASDLKTAG 372
                        F +Q  E     Q    +L      KP + E+ T  +PS SDL  AG
Sbjct: 317 PGFMALKLSADYLFTRQENEATTTRQESVSILDI---EKPPLVEELT--IPSVSDLHKAG 376

Query: 373 ITFKSTENG-ILKCRFDETTLTLTLPSIHLDGFSGVLLKNLVAFEAMAELNPPCLGVYIA 432
           + FK T +G I    FD  +    LP I+LD  +  +L+NLVA+EA     P     Y  
Sbjct: 377 VRFKPTAHGNISTVTFDSNSGQFYLPVINLDINTETVLRNLVAYEATNTSGPLVFTRYTE 436

Query: 433 LMNGLLRNSKDLKILEKEEIVHNHLNSEEEAVRLFNGVENSTSSLKKESVLKIHLKSGCD 492
           L+NG++ + +D+++L ++ ++ + L S++EA  ++NG+  S                   
Sbjct: 437 LINGIIDSEEDVRLLREQGVLVSRLKSDQEAAEMWNGMSKS------------------- 496

Query: 493 LEIESKFIQDIRTNYEMGLGAMVEEINKCYESCWRMKMKKFVSVVL----KCFAILVVVF 513
                     +R      L   +E++N+ Y   W++K+ + V V +    +  A L  V 
Sbjct: 497 ----------VRLTKVGFLDKTIEDVNRYYTGRWKVKIGRLVEVYVYGSWQILAFLAAVL 511

BLAST of Spg021117 vs. TAIR 10
Match: AT3G50180.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 89.4 bits (220), Expect = 1.3e-17
Identity = 60/203 (29.56%), Postives = 105/203 (51.72%), Query Frame = 0

Query: 16  QWVLQVNDLLDQ--NALPAASQTPISVFQLPETVRNGDPEAYLPRRLAVGPYHHFQPELY 75
           +WV+ + D L+Q        S   + ++++P  +   D ++Y P+ +++GPYHH + +  
Sbjct: 154 EWVISIKDKLEQAYRNDDRTSWGKLCIYKVPHYLHGNDKKSYFPQTVSLGPYHHGRQQTQ 213

Query: 76  KMDLFKLGRAKNL---KWSPQIHKLADQLNPLELKIRACFDQSLDIDGETLSWVVLIDGL 135
            M+  K  RA N+   + +  I    D +  LE K RAC++ S+ +     + ++L+DG 
Sbjct: 214 SMECHK-WRAVNMVLKRTNQGIEVFLDAMIELEEKARACYEGSIVLSSNEFTEMLLLDGC 273

Query: 136 FLLYLLQNKYDNRPLKL------PLEIFYGKLWSEFEIVSDMVKLENQIPLFVLKEICPK 195
           F+L LLQ   +   LKL      P+    G + S   I  DM+ LENQ+PLFVL  +   
Sbjct: 274 FILELLQGVNEGF-LKLGYDHNDPVFAVRGSMHS---IQRDMIMLENQLPLFVLNRLLEL 333

Query: 196 EP----RNYLAPLLYKFYVSISP 204
           +P    +  L  L+ +F++ + P
Sbjct: 334 QPGTQNQTGLVELVVRFFIPLMP 351

BLAST of Spg021117 vs. TAIR 10
Match: AT5G22550.2 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 80.9 bits (198), Expect = 4.5e-15
Identity = 103/419 (24.58%), Postives = 174/419 (41.53%), Query Frame = 0

Query: 40  VFQLPETVRNGDPEAYLPRRLAVGPYHHFQP-------ELYKMDLFKLGRAKNLKWSPQI 99
           ++++P T++  + +AY P+ +++GPYHH          E +K    ++  +K  +    +
Sbjct: 46  IYRIPHTLKQVNDKAYAPKIVSIGPYHHSSDKQHLKMIEEHKKRYLEMFVSKTKENGVYL 105

Query: 100 HKLADQLNPLELKIRACFDQSLDIDGETLSWVVLIDGLFLLYL---LQNKYDNRPLKLPL 159
             L D ++ LE KIR  + ++L+   + L  V+L+DG F+L L   +  K +   LK P+
Sbjct: 106 IHLVDLVSGLEQKIRDSYSENLEFSQQKLIKVMLLDGCFILMLFLVVSQKIEYTNLKDPI 165

Query: 160 EIFYGKLWSEFEIVSDMVKLENQIPLFVLK---EICPKEPRNYLAPLLYKFYVSISPFKL 219
              +   W    + SD++ LENQ+PLF+LK   E     P   L  L +KF+        
Sbjct: 166 ---FKLRWILPTLRSDLLLLENQVPLFLLKVLLETSKLAPSTSLNMLAFKFF-------- 225

Query: 220 PSFNIELPQIFDLSHHLLHFLYFLILLIPNEMVVAVIAEMETGDVPISIGMDSNSSSSSS 279
             ++I+ P+ F   H+ L   + L+ LI    + A           I+I       S + 
Sbjct: 226 -DYSIKKPEGFWEKHNNLRAKH-LLDLIRKTFIPAPPPSTTPRQCCINIFNGPREYSRTE 285

Query: 280 SVNFLSEFLNILGSVINIAFLQQVKETFGLIQRLFRLLGFITKPKPNVSEKKTRSVPSAS 339
           +        NI    I+ +     KE  G            + P P         + SA 
Sbjct: 286 TSK------NICLGKISCS-----KEITG---------AQTSSPPPPPRRPFLGLIVSAR 345

Query: 340 DLKTAGITFKSTENGILKCRFDETTLTLTLPSIHLDGFSGVLLKNLVAFEAMAELNPPC- 399
            L+  GI F   EN          +  + +P +  D F   LL N VAFE   + N  C 
Sbjct: 346 KLRLRGIKFMRKENVETPLDISFKSGLVEIPLLVFDDFISNLLINCVAFE---QFNMSCS 405

Query: 400 --LGVYIALMNGLLRNSKDLKILEKEEIVHNHLNSEEEAVRLFNGVENSTSSLKKESVL 443
             +  ++  M  L+    D   L ++ I+ N+  + EE    F  +    S    +S L
Sbjct: 406 TEITSFVIFMGCLINTEDDATFLIEKGILENYFGTGEEVSLFFKNIGKDISFSISKSFL 428

BLAST of Spg021117 vs. TAIR 10
Match: AT3G47250.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 79.7 bits (195), Expect = 1.0e-14
Identity = 94/408 (23.04%), Postives = 163/408 (39.95%), Query Frame = 0

Query: 30  LPAASQTPISVFQLPETVRNGDPEAYLPRRLAVGPYHHFQPEL-----YKMDLFKLGRAK 89
           L +A +    +F++P+++   +P+AY P+ +++GPYH+ +  L     +K    +L   +
Sbjct: 53  LESAGKASCCIFRIPDSLAEVNPKAYKPKVVSIGPYHYGENHLQMIQQHKFRFLELFVDR 112

Query: 90  NLKWSPQIHKLADQLNPLELKIRACFDQSLDIDGETLSWVVLIDGLFLLYLLQNKYDNRP 149
             K     + L   +  L+ KIRA + + L I+   L  ++++DG F+L LL        
Sbjct: 113 ATKKGMDENVLYAAVGALQHKIRASYSEELRIEKSELVSMMILDGCFILMLLLIVSRKID 172

Query: 150 LKLPLEIFYGKLWSEFEIVSDMVKLENQIPLFVLKEICPKEPRNYLAPLLYKFYVSISPF 209
           L +  +  +   W    I SD++ LENQ+P FVL+ I  K        L      +   F
Sbjct: 173 LDMNKDPIFTIPWILASIQSDLLLLENQVPFFVLRTIFDKSGIGSPGDL------NRMAF 232

Query: 210 KLPSFNIELPQIFDLSHHLLHFLYFLILLIPNEMVVAVIAEMETGDVPISIGMDSNSSSS 269
              + +I+ P  +   H      + L                   D+     + S  S  
Sbjct: 233 SFFNLSIDKPDTYWAKHRDRGAKHLL-------------------DLNRKTFIPSMRSMG 292

Query: 270 SSSVNFLSEFLNILGSVINIAFLQQVKETFGLIQRLFRLLGFITKPKPNVSEKKTRSVPS 329
            S     S+F +  G    ++     + TF LI                          S
Sbjct: 293 ESETTPSSKFQHSKGKSGEVS---SSESTFPLIL-------------------------S 352

Query: 330 ASDLKTAGITF--KSTENGILKCRFDETTLTLTLPSIHLDGFSGVLLKNLVAFEAMAELN 389
           A  L+  GI F  +S    IL  +  +    L +P + LDGF   +  N VAFE     +
Sbjct: 353 AKRLRLQGIKFRLRSDAESILDIKLKKN--KLQIPLLRLDGFISSIFLNCVAFEQFYTES 405

Query: 390 PPCLGVYIALMNGLLRNSKDLKILEKEE-IVHNHLNSEEEAVRLFNGV 430
              +  Y+  M  LL + +D   L  ++ I+ N+  +E E  + F  +
Sbjct: 413 TNDITSYVVFMGCLLNDQEDATFLNNDKRIIENYFGNENEVSQFFKTI 405

BLAST of Spg021117 vs. TAIR 10
Match: AT3G47250.2 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 79.7 bits (195), Expect = 1.0e-14
Identity = 94/408 (23.04%), Postives = 163/408 (39.95%), Query Frame = 0

Query: 30  LPAASQTPISVFQLPETVRNGDPEAYLPRRLAVGPYHHFQPEL-----YKMDLFKLGRAK 89
           L +A +    +F++P+++   +P+AY P+ +++GPYH+ +  L     +K    +L   +
Sbjct: 53  LESAGKASCCIFRIPDSLAEVNPKAYKPKVVSIGPYHYGENHLQMIQQHKFRFLELFVDR 112

Query: 90  NLKWSPQIHKLADQLNPLELKIRACFDQSLDIDGETLSWVVLIDGLFLLYLLQNKYDNRP 149
             K     + L   +  L+ KIRA + + L I+   L  ++++DG F+L LL        
Sbjct: 113 ATKKGMDENVLYAAVGALQHKIRASYSEELRIEKSELVSMMILDGCFILMLLLIVSRKID 172

Query: 150 LKLPLEIFYGKLWSEFEIVSDMVKLENQIPLFVLKEICPKEPRNYLAPLLYKFYVSISPF 209
           L +  +  +   W    I SD++ LENQ+P FVL+ I  K        L      +   F
Sbjct: 173 LDMNKDPIFTIPWILASIQSDLLLLENQVPFFVLRTIFDKSGIGSPGDL------NRMAF 232

Query: 210 KLPSFNIELPQIFDLSHHLLHFLYFLILLIPNEMVVAVIAEMETGDVPISIGMDSNSSSS 269
              + +I+ P  +   H      + L                   D+     + S  S  
Sbjct: 233 SFFNLSIDKPDTYWAKHRDRGAKHLL-------------------DLNRKTFIPSMRSMG 292

Query: 270 SSSVNFLSEFLNILGSVINIAFLQQVKETFGLIQRLFRLLGFITKPKPNVSEKKTRSVPS 329
            S     S+F +  G    ++     + TF LI                          S
Sbjct: 293 ESETTPSSKFQHSKGKSGEVS---SSESTFPLIL-------------------------S 352

Query: 330 ASDLKTAGITF--KSTENGILKCRFDETTLTLTLPSIHLDGFSGVLLKNLVAFEAMAELN 389
           A  L+  GI F  +S    IL  +  +    L +P + LDGF   +  N VAFE     +
Sbjct: 353 AKRLRLQGIKFRLRSDAESILDIKLKKN--KLQIPLLRLDGFISSIFLNCVAFEQFYTES 405

Query: 390 PPCLGVYIALMNGLLRNSKDLKILEKEE-IVHNHLNSEEEAVRLFNGV 430
              +  Y+  M  LL + +D   L  ++ I+ N+  +E E  + F  +
Sbjct: 413 TNDITSYVVFMGCLLNDQEDATFLNNDKRIIENYFGNENEVSQFFKTI 405