Spg020093 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg020093 vs. NCBI nr
Match: KAG7025549.1 (Ankyrin-1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 771.2 bits (1990), Expect = 5.2e-219
Identity = 390/459 (84.97%), Postives = 423/459 (92.16%), Query Frame = 0

Query: 1   MDKLVRPDVKEVEFSFTKGEKCTVTFSLANLMHTMSVAVCLSTSNPSVFSFSQNFSIIPP 60
           MD+LV+PDVKEVEF FTKGEKC+ TF+LANLMHTMSVAVCL++SNPSVFSFSQ+FS+IPP
Sbjct: 1   MDRLVKPDVKEVEFCFTKGEKCSATFTLANLMHTMSVAVCLTSSNPSVFSFSQDFSVIPP 60

Query: 61  LSSSSYTISCKSLDQPPLSTPPDKLSVRSAMLPIGKAHTDDLRRLFSKPGRHIFKDASLL 120
           LSSSSYTISCKS DQPPLS P DK+SVRSAM+PIG AHTDDLR LFSKPGRHIFKDA LL
Sbjct: 61  LSSSSYTISCKSSDQPPLSNPSDKISVRSAMVPIGNAHTDDLRLLFSKPGRHIFKDALLL 120

Query: 121 ISFIGHDVVEFLISHHNRIPDLSFLLNKAISGCTTSQLAALLKPAISSGKLTLVSALIDA 180
           ISF+G DVVEFLISHHNRI +LSFLLNKAIS CT SQL AL++PAI SGKL LVSALIDA
Sbjct: 121 ISFVGPDVVEFLISHHNRITELSFLLNKAISSCTKSQLTALMEPAILSGKLGLVSALIDA 180

Query: 181 GVDVNDKDSLKRSMMSLAVRTGKIDIVKRLIDSHCQVDFSVDLVLHISAAMNRIDLIELL 240
           GVDVN KDSLKRSMMSLAVRTGKIDIVKRLIDSHCQVDFSVDLVLHI+A+MNR+D +ELL
Sbjct: 181 GVDVNVKDSLKRSMMSLAVRTGKIDIVKRLIDSHCQVDFSVDLVLHIAASMNRVDFMELL 240

Query: 241 HRKFLEIPVNSVDSDGRTPIHTAAAHGHVEAIRFLISIGGDPTAVDWEKWTPLHSAAAEG 300
            + F +IPVNSVDS+GRTPIHTAAA GHVEAIRFL+SIGG+P AVD  KWTPLHSAAAEG
Sbjct: 241 LKNFPDIPVNSVDSNGRTPIHTAAARGHVEAIRFLLSIGGNPEAVDRNKWTPLHSAAAEG 300

Query: 301 HFEAVEYLLSCSNVKYAVNSDGRTAFALAAENGHADLFDLLRLGDALHRTARAGDVRGLR 360
           HFEAVEY L+CSNVKYAVNSDG+TAFALA+ENGH DLFD LRLGDALHR ARAGDVRGLR
Sbjct: 301 HFEAVEYFLNCSNVKYAVNSDGKTAFALASENGHTDLFDSLRLGDALHRAARAGDVRGLR 360

Query: 361 SCVAAGANVNGKDQNGWTALHRAAFKGRVECIKALLEHGADADAVDDAGYTPLRCAVEAG 420
           SCVAAGA +NGKDQNGWTALHRAAFKGR+EC+KALLEHGAD DAVDDAGYTPLRCAVEAG
Sbjct: 361 SCVAAGAKINGKDQNGWTALHRAAFKGRIECVKALLEHGADTDAVDDAGYTPLRCAVEAG 420

Query: 421 QEEVARLLLASGAKSNSLRGLKRGLIVSGRQISSLCYGN 460
           QEEVAR+LLASGA+ N L+ L+R L VSGRQISSLC GN
Sbjct: 421 QEEVARVLLASGAEPNPLKCLRRDLAVSGRQISSLCCGN 459

BLAST of Spg020093 vs. NCBI nr
Match: XP_022960469.1 (ankyrin repeat domain-containing protein 50-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 769.6 bits (1986), Expect = 1.5e-218
Identity = 390/459 (84.97%), Postives = 423/459 (92.16%), Query Frame = 0

Query: 1   MDKLVRPDVKEVEFSFTKGEKCTVTFSLANLMHTMSVAVCLSTSNPSVFSFSQNFSIIPP 60
           MD+LV+PDVKEVEF FTKGEKC+ +F+LANLMHTMSVAVCL++SNPSVFSFSQ+FS+IPP
Sbjct: 1   MDRLVKPDVKEVEFFFTKGEKCSASFTLANLMHTMSVAVCLTSSNPSVFSFSQDFSVIPP 60

Query: 61  LSSSSYTISCKSLDQPPLSTPPDKLSVRSAMLPIGKAHTDDLRRLFSKPGRHIFKDASLL 120
           LSSSSYTISCKS DQPPLS P DK+SVRSAM+PIG AHTDDLR LFSKPGRHIFKDA LL
Sbjct: 61  LSSSSYTISCKSSDQPPLSNPSDKISVRSAMVPIGNAHTDDLRLLFSKPGRHIFKDALLL 120

Query: 121 ISFIGHDVVEFLISHHNRIPDLSFLLNKAISGCTTSQLAALLKPAISSGKLTLVSALIDA 180
           ISF+G DVVEFLISHHNRI +LSFLLNKAIS CT SQL AL++PAI SGKL LVSALIDA
Sbjct: 121 ISFVGPDVVEFLISHHNRITELSFLLNKAISSCTKSQLTALMEPAILSGKLGLVSALIDA 180

Query: 181 GVDVNDKDSLKRSMMSLAVRTGKIDIVKRLIDSHCQVDFSVDLVLHISAAMNRIDLIELL 240
           GVDVN KDSLKRSMMSLAVRTGKIDIVKRLIDSHCQVDFSVDLVLHI+A+MNR+D +ELL
Sbjct: 181 GVDVNVKDSLKRSMMSLAVRTGKIDIVKRLIDSHCQVDFSVDLVLHIAASMNRVDFMELL 240

Query: 241 HRKFLEIPVNSVDSDGRTPIHTAAAHGHVEAIRFLISIGGDPTAVDWEKWTPLHSAAAEG 300
            + F +IPVNSVDS+GRTPIHTAAA GHVEAIRFL+SIGG+P AVD  KWTPLHSAAAEG
Sbjct: 241 LKNFPDIPVNSVDSNGRTPIHTAAARGHVEAIRFLLSIGGNPEAVDRNKWTPLHSAAAEG 300

Query: 301 HFEAVEYLLSCSNVKYAVNSDGRTAFALAAENGHADLFDLLRLGDALHRTARAGDVRGLR 360
           HFEAVEY L+CSNVKYAVNSDG+TAFALA+ENGH DLFD LRLGDALHR ARAGDVRGLR
Sbjct: 301 HFEAVEYFLNCSNVKYAVNSDGKTAFALASENGHTDLFDSLRLGDALHRAARAGDVRGLR 360

Query: 361 SCVAAGANVNGKDQNGWTALHRAAFKGRVECIKALLEHGADADAVDDAGYTPLRCAVEAG 420
           SCVAAGA +NGKDQNGWTALHRAAFKGRVEC+KALLEHGAD DAVDDAGYTPLRCAVEAG
Sbjct: 361 SCVAAGAKINGKDQNGWTALHRAAFKGRVECVKALLEHGADTDAVDDAGYTPLRCAVEAG 420

Query: 421 QEEVARLLLASGAKSNSLRGLKRGLIVSGRQISSLCYGN 460
           QEEVAR+LLASGA+ N L+ L+R L VSGRQISSLC GN
Sbjct: 421 QEEVARVLLASGAEPNPLKCLRRDLAVSGRQISSLCCGN 459

BLAST of Spg020093 vs. NCBI nr
Match: KAG6593150.1 (Ankyrin-1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 768.8 bits (1984), Expect = 2.6e-218
Identity = 389/459 (84.75%), Postives = 422/459 (91.94%), Query Frame = 0

Query: 1   MDKLVRPDVKEVEFSFTKGEKCTVTFSLANLMHTMSVAVCLSTSNPSVFSFSQNFSIIPP 60
           MD+LV+PDVKEVEF FTKGEKC+ TF+LANLMHTMSVAVCL++SNPSVFSFSQ+FS+IPP
Sbjct: 1   MDRLVKPDVKEVEFCFTKGEKCSATFTLANLMHTMSVAVCLTSSNPSVFSFSQDFSVIPP 60

Query: 61  LSSSSYTISCKSLDQPPLSTPPDKLSVRSAMLPIGKAHTDDLRRLFSKPGRHIFKDASLL 120
           LSSSSYTISCKS DQPPLS P DK+SVRSAM+PIG AHTDDLR LFSKPGRHIFKDA LL
Sbjct: 61  LSSSSYTISCKSSDQPPLSNPSDKISVRSAMVPIGNAHTDDLRLLFSKPGRHIFKDALLL 120

Query: 121 ISFIGHDVVEFLISHHNRIPDLSFLLNKAISGCTTSQLAALLKPAISSGKLTLVSALIDA 180
           ISF+G DVVEFLISHHNRI +LSFLLNKAIS CT SQL AL++PAI SGKL LVSALIDA
Sbjct: 121 ISFVGPDVVEFLISHHNRITELSFLLNKAISSCTKSQLTALMEPAIFSGKLGLVSALIDA 180

Query: 181 GVDVNDKDSLKRSMMSLAVRTGKIDIVKRLIDSHCQVDFSVDLVLHISAAMNRIDLIELL 240
           GVDVN KDSLKRSMMSLAVRTGKIDIVKRLIDSHCQVDFSVDLVLHI+A+MNR+D +ELL
Sbjct: 181 GVDVNVKDSLKRSMMSLAVRTGKIDIVKRLIDSHCQVDFSVDLVLHIAASMNRVDFMELL 240

Query: 241 HRKFLEIPVNSVDSDGRTPIHTAAAHGHVEAIRFLISIGGDPTAVDWEKWTPLHSAAAEG 300
            + F +IPVN VDS+GRTPIHTAAA GHVEAIRFL+SIGG+P AVD  KWTPLHSAAAEG
Sbjct: 241 LKNFPDIPVNFVDSNGRTPIHTAAARGHVEAIRFLLSIGGNPEAVDRNKWTPLHSAAAEG 300

Query: 301 HFEAVEYLLSCSNVKYAVNSDGRTAFALAAENGHADLFDLLRLGDALHRTARAGDVRGLR 360
           HFEAVEY L+CSNVKYAVNSDG+TAFALA+ENGH DLFD LRLGDALHR ARAGDVRGLR
Sbjct: 301 HFEAVEYFLNCSNVKYAVNSDGKTAFALASENGHTDLFDSLRLGDALHRAARAGDVRGLR 360

Query: 361 SCVAAGANVNGKDQNGWTALHRAAFKGRVECIKALLEHGADADAVDDAGYTPLRCAVEAG 420
           SCVAAGA +NGKDQNGWTALHRAAFKGR+EC+KALLEHGAD DAVDDAGYTPLRCAVEAG
Sbjct: 361 SCVAAGAKINGKDQNGWTALHRAAFKGRIECVKALLEHGADTDAVDDAGYTPLRCAVEAG 420

Query: 421 QEEVARLLLASGAKSNSLRGLKRGLIVSGRQISSLCYGN 460
           QEEVAR+LLASGA+ N L+ L+R L VSGRQISSLC GN
Sbjct: 421 QEEVARVLLASGAEPNPLKCLRRDLAVSGRQISSLCCGN 459

BLAST of Spg020093 vs. NCBI nr
Match: XP_023513846.1 (ankyrin repeat domain-containing protein 50-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 768.5 bits (1983), Expect = 3.3e-218
Identity = 388/459 (84.53%), Postives = 422/459 (91.94%), Query Frame = 0

Query: 1   MDKLVRPDVKEVEFSFTKGEKCTVTFSLANLMHTMSVAVCLSTSNPSVFSFSQNFSIIPP 60
           MD+LV+PDVKEVEF FTKGEKC+ TF+LANLMHTMSVAVCL++SNPSVFSFSQ+FS+IPP
Sbjct: 28  MDRLVKPDVKEVEFCFTKGEKCSATFTLANLMHTMSVAVCLTSSNPSVFSFSQDFSVIPP 87

Query: 61  LSSSSYTISCKSLDQPPLSTPPDKLSVRSAMLPIGKAHTDDLRRLFSKPGRHIFKDASLL 120
           LSSSSYTISCKS DQPPLS P DK+SVRSAM+PIG AHTDDLR LFSKPGRHIFKDA LL
Sbjct: 88  LSSSSYTISCKSSDQPPLSNPSDKISVRSAMVPIGNAHTDDLRLLFSKPGRHIFKDALLL 147

Query: 121 ISFIGHDVVEFLISHHNRIPDLSFLLNKAISGCTTSQLAALLKPAISSGKLTLVSALIDA 180
           ISF+G DVVEFLISHHNRI +LSFLLNKAIS CT SQL AL++PAI SGKL LVSALIDA
Sbjct: 148 ISFVGPDVVEFLISHHNRITELSFLLNKAISSCTKSQLTALMEPAILSGKLGLVSALIDA 207

Query: 181 GVDVNDKDSLKRSMMSLAVRTGKIDIVKRLIDSHCQVDFSVDLVLHISAAMNRIDLIELL 240
           GVDVN KDSLKRSMMSLAVRTGKIDIVKRLIDSHCQ+DFSVDLVLHI+A+MNR+D +ELL
Sbjct: 208 GVDVNVKDSLKRSMMSLAVRTGKIDIVKRLIDSHCQIDFSVDLVLHIAASMNRVDFMELL 267

Query: 241 HRKFLEIPVNSVDSDGRTPIHTAAAHGHVEAIRFLISIGGDPTAVDWEKWTPLHSAAAEG 300
            +KF +IPVNS+DS+GRTPIHTAA  GHVEAIRFL+SIGG P AVD  KWTPLHSAAAEG
Sbjct: 268 VKKFPDIPVNSIDSNGRTPIHTAADRGHVEAIRFLLSIGGIPEAVDRNKWTPLHSAAAEG 327

Query: 301 HFEAVEYLLSCSNVKYAVNSDGRTAFALAAENGHADLFDLLRLGDALHRTARAGDVRGLR 360
           HFEAVEY L+CSNVKYAVNSDG+TAFALA+ENGH DLFD LRLGDALHR ARAGDVRGLR
Sbjct: 328 HFEAVEYFLNCSNVKYAVNSDGKTAFALASENGHTDLFDSLRLGDALHRAARAGDVRGLR 387

Query: 361 SCVAAGANVNGKDQNGWTALHRAAFKGRVECIKALLEHGADADAVDDAGYTPLRCAVEAG 420
           SCVAAGA +NGKDQNGWTALHRAAFKGRVEC+KALLEHGAD DA+DDAGYTPLRCAVEAG
Sbjct: 388 SCVAAGAKINGKDQNGWTALHRAAFKGRVECVKALLEHGADTDAIDDAGYTPLRCAVEAG 447

Query: 421 QEEVARLLLASGAKSNSLRGLKRGLIVSGRQISSLCYGN 460
           QEEVAR+LLASGA+ N L+ L+R L VSGRQISSLC GN
Sbjct: 448 QEEVARVLLASGAEPNPLKCLRRDLAVSGRQISSLCCGN 486

BLAST of Spg020093 vs. NCBI nr
Match: XP_038896354.1 (protein VAPYRIN-LIKE-like [Benincasa hispida])

HSP 1 Score: 758.4 bits (1957), Expect = 3.5e-215
Identity = 382/458 (83.41%), Postives = 418/458 (91.27%), Query Frame = 0

Query: 1   MDKLVRPDVKEVEFSFTKGEKCTVTFSLANLMHTMSVAVCLSTSNPSVFSFSQNFSIIPP 60
           MD+LV+PDVKEVEFSF K E C+ TF L NLMHTMSVAVCLSTSNPSVFSFSQ+FS+IPP
Sbjct: 1   MDRLVKPDVKEVEFSFVKAENCSATFCLTNLMHTMSVAVCLSTSNPSVFSFSQDFSVIPP 60

Query: 61  LSSSSYTISCKSLDQPPLSTPPDKLSVRSAMLPIGKAHTDDLRRLFSKPGRHIFKDASLL 120
           LSSSSYTISCKS ++PPLSTPPDK+SVRSAMLPIGKAHTDDLRRLFS PGRH+FKDASLL
Sbjct: 61  LSSSSYTISCKSSEKPPLSTPPDKISVRSAMLPIGKAHTDDLRRLFSSPGRHVFKDASLL 120

Query: 121 ISFIGHDVVEFLISHHNRIPDLSFLLNKAISGCTTSQLAALLKPAISSGKLTLVSALIDA 180
           ISF+G DVVEFLIS+H RIPDL FLLNKAISGCT SQL AL++PAISSGKL LVSALIDA
Sbjct: 121 ISFVGFDVVEFLISNHKRIPDLRFLLNKAISGCTKSQLTALMEPAISSGKLGLVSALIDA 180

Query: 181 GVDVNDKDSLKRSMMSLAVRTGKIDIVKRLIDSHCQVDFSVDLVLHISAAMNRIDLIELL 240
           GVDVN KDSLKRSM+S+AVR G IDIVKRLIDSHC++DFSVDLVLHI+A MNR+DLI+LL
Sbjct: 181 GVDVNVKDSLKRSMLSIAVRIGNIDIVKRLIDSHCEIDFSVDLVLHIAATMNRVDLIDLL 240

Query: 241 HRKFLEIPVNSVDSDGRTPIHTAAAHGHVEAIRFLISIGGDPTAVDWEKWTPLHSAAAEG 300
           H  F +IPVNSVDSDGRTPIH AAA+GHVEA+R+L +IGGDP AVD +KWTPLH AAAEG
Sbjct: 241 HENFPKIPVNSVDSDGRTPIHVAAANGHVEAMRYLTTIGGDPEAVDRKKWTPLHFAAAEG 300

Query: 301 HFEAVEYLLSCSNVKYAVNSDGRTAFALAAENGHADLFDLLRLGDALHRTARAGDVRGLR 360
           H EAVEYLL+ SNVKYAVNSDGRTAFALA+ENGH DLFD LRL DALHRTARAGDVRGLR
Sbjct: 301 HLEAVEYLLNWSNVKYAVNSDGRTAFALASENGHTDLFDSLRLDDALHRTARAGDVRGLR 360

Query: 361 SCVAAGANVNGKDQNGWTALHRAAFKGRVECIKALLEHGADADAVDDAGYTPLRCAVEAG 420
           SCVAAGANVNGKDQNGWTALHRAAFKGR+EC+KALLEHGA+ DAVD+AGYTPLRCAVEAG
Sbjct: 361 SCVAAGANVNGKDQNGWTALHRAAFKGRIECVKALLEHGAETDAVDNAGYTPLRCAVEAG 420

Query: 421 QEEVARLLLASGAKSNSLRGLKRGLIVSGRQISSLCYG 459
           QEEVARLL+ SGAK N L+GL+R L V G +ISSLC G
Sbjct: 421 QEEVARLLVESGAKPNLLKGLRRDLNVCGGEISSLCCG 458

BLAST of Spg020093 vs. ExPASy Swiss-Prot
Match: D3J163 (Protein VAPYRIN-LIKE OS=Medicago truncatula OX=3880 GN=VPYL PE=2 SV=1)

HSP 1 Score: 451.1 bits (1159), Expect = 1.5e-125
Identity = 228/443 (51.47%), Postives = 317/443 (71.56%), Query Frame = 0

Query: 1   MDKLVRPDVKEVEFSFTKGEKCTVTFSLANLMHTMSVAVCLSTSNPSVFSFSQNFSIIPP 60
           MD+LV+ +  EV  +F K +KC+ +F L NLMHTMSVAV L+T+NP+ FS ++  S+IPP
Sbjct: 1   MDRLVKTEFNEVNLNFQKNQKCSSSFKLTNLMHTMSVAVSLTTTNPTTFSINKPLSVIPP 60

Query: 61  LSSSSYTISCKSLDQPPLSTPPDKLSVRSAMLPIGKAHTDDLRRLFSKPGRHIFKDASLL 120
           LSSS+YT+   +L+QPPLS P D ++VR++MLP GKA TDDLRRLF+KPG H+F+DA + 
Sbjct: 61  LSSSTYTLHLTNLNQPPLSEPADVITVRTSMLPTGKATTDDLRRLFNKPGPHVFRDAVIT 120

Query: 121 ISFIGHDVVEFLISHHNRIPDLSFLLNKAISGCTTSQLAALLKPAISSGKLTLVSALIDA 180
           +  +G  V E++IS++    +   L  KAIS CT S L  L+KPA+ SGK+  V+ LI A
Sbjct: 121 VILVGPTVAEYVISNY----ETRNLFTKAISVCTKSNLTNLMKPAVESGKVEYVTDLITA 180

Query: 181 GVDVNDKDSLKRSMMSLAVRTGKIDIVKRLIDSHCQVDFSVDLVLHISAAMNRIDLIELL 240
           G DVN +DS  +S++  A+RTGK+ ++K L+ + C+++ SVD VLH +A ++R+D+++ L
Sbjct: 181 GGDVNFRDSNGKSLIPFAIRTGKLAVLKLLVANGCRINDSVDFVLHEAAIIDRVDVVKFL 240

Query: 241 HRKFL-EIPVNSVDSDGRTPIHTAAAHGHVEAIRFLISIGGDPTAVDWEKWTPLHSAAAE 300
              F  E+ VNSV+ +  TPIH +A+ GHV  I F +SIGG+  AVD  +WTPLH AA+ 
Sbjct: 241 FESFCDELDVNSVNREMMTPIHVSASEGHVSLIEFFVSIGGNANAVDSRRWTPLHHAASR 300

Query: 301 GHFEAVEYLLSCSNVKYAVNSDGRTAFALAAENGHADLFDLLRLGDALHRTARAGDVRGL 360
            H +AVE+LL  S+VKYA   +G+TAF +A+E+GH  LF +LR GDAL + AR  DV  L
Sbjct: 301 NHLKAVEFLLENSDVKYARELNGKTAFEIASESGHTRLFGVLRWGDALLQAARVDDVHAL 360

Query: 361 RSCVAAGANVNGKDQNGWTALHRAAFKGRVECIKALLEHGADADAVDDAGYTPLRCAVEA 420
           + C+  GA VN KDQNGWT LH A+FKGR++ +K LLEHGA+ D+VDDAGYTPL CA EA
Sbjct: 361 KKCLGEGAEVNRKDQNGWTPLHWASFKGRIKSVKVLLEHGAEVDSVDDAGYTPLHCAAEA 420

Query: 421 GQEEVARLLLASGAKSNSLRGLK 443
           G  +VA +L+A G    +L+  +
Sbjct: 421 GHLQVALVLIAHGGCQTNLKSFQ 439

BLAST of Spg020093 vs. ExPASy Swiss-Prot
Match: C7B178 (Protein VAPYRIN OS=Petunia hybrida OX=4102 GN=VPY PE=2 SV=1)

HSP 1 Score: 204.1 bits (518), Expect = 3.3e-51
Identity = 153/457 (33.48%), Postives = 232/457 (50.77%), Query Frame = 0

Query: 1   MDKLVRPDVKE-VEFSFTKGEKCTVTFSLANLMHTMSVAVCLSTSNPSVFSFSQNFSIIP 60
           MD+L+  +    V      G+KC+   +L N+M+TM VA  L   N   +S      II 
Sbjct: 1   MDRLLSLEPSNVVTIRLEPGQKCSGVLTLRNVMYTMPVAFRLQPLNKIRYSIRPQSGIIS 60

Query: 61  PLSSSSYTISCKSLDQPPLSTPPDKLS-------VRSAMLPIGKAHTDDLRRL------- 120
           PL++ +  I       PP +T PD          + S + P G    D    L       
Sbjct: 61  PLTTITLEI---IYHLPPNTTLPDSFPHCDDSFLLHSVVAP-GATAKDTSSTLDMVPSDW 120

Query: 121 FSKPGRHIFKDASLLISFIGHDVVEFLISHHNRIPDLSFLLNKAIS-----GCTTSQLAA 180
           F+   + +F D+++ I F+G  V+ +L+     + ++  +L K+ +          +   
Sbjct: 121 FTTKRKQVFIDSAIKIMFVGSPVLCYLV-RKGYMDEIREVLEKSDTTWKSVDSVNFEGQT 180

Query: 181 LLKPAISSGKLTLVSALIDAGVDVNDKDSLKRSMMSLAVRTGKIDIVKRLIDSHC---QV 240
           LL  AIS G+  LV  L++ G ++        S +  A  TG+  IV+ L+       + 
Sbjct: 181 LLHLAISQGRPDLVQLLLEFGPNIEAHSRSCSSPLEAASATGEALIVELLLAKKASTERT 240

Query: 241 DFSVDLVLHISAAMNRIDLIELLHRKFLEIPVNSVDSDGRTPIHTAAAHGHVEAIRFLIS 300
           +FS    +H++A    +++++LL  K     VNS+  DG T +H A      +  R L++
Sbjct: 241 EFSASGPIHLAAGNGHLEVLKLLLLKGAN--VNSLTKDGNTALHLAVEERRRDCARLLLA 300

Query: 301 IGGDPTAVDWEKW-TPLHSAAAEGHFEAVEYLLSCSNVKYAVNSDGRTAFALAAENGHAD 360
            G            TPLH AA  G    V  LL     KY  N  G+TA+ +AAE+GH  
Sbjct: 301 NGARADICSTGNGDTPLHIAAGLGDEHMVRVLLQKGAEKYIRNKYGKTAYDVAAEHGHNK 360

Query: 361 LFDLLRLGDALHRTARAGDVRGLRSCVAAGANVNGKDQNGWTALHRAAFKGRVECIKALL 420
           LFD LRLGD+L   AR G+VR ++  +  GA++NG+DQ+GWTALHRA FKGR+E +KAL+
Sbjct: 361 LFDALRLGDSLCVAARKGEVRTVQRLLENGASINGRDQHGWTALHRACFKGRIEVVKALI 420

Query: 421 EHGADADAVDDAGYTPLRCAVEAGQEEVARLLLASGA 434
           ++G D +A D+ GYT L CAVE+G  +VA LL+  GA
Sbjct: 421 DNGIDVNARDEDGYTALHCAVESGHVDVAELLVKKGA 450

BLAST of Spg020093 vs. ExPASy Swiss-Prot
Match: D3J162 (Protein VAPYRIN OS=Medicago truncatula OX=3880 GN=VPY PE=1 SV=1)

HSP 1 Score: 198.7 bits (504), Expect = 1.4e-49
Identity = 149/457 (32.60%), Postives = 229/457 (50.11%), Query Frame = 0

Query: 1   MDKLVRPDVKE-VEFSFTKGEKCTVTFSLANLMHTMSVAVCLSTSNPSVFSFSQNFSIIP 60
           MD+L++ D    V     +G+KC    +L N+M+TM VA  +     + ++      II 
Sbjct: 1   MDRLIKLDPSNIVLIRVEEGQKCLGKITLNNVMYTMPVAFRIQPLIKTRYTIKPQSGIIS 60

Query: 61  PLSSSSYTISCKSLDQP-----PLSTP--PDKLSVRSAMLPIGKAHTDDLRRLFSKPG-- 120
           PL+S    I+     Q      P S P   D   + S + P G A  +      S P   
Sbjct: 61  PLASLVIEITYHPPQQQGSNNLPHSFPFSDDSFLLHSVLAP-GAAIKEPSSMFDSVPSDW 120

Query: 121 -----RHIFKDASLLISFIGHDVVEFLISHHNRIPDLSFLLNKA-----ISGCTTSQLAA 180
                + +F D+++ + F+G  ++  L+   N + D+  +L K+           SQ   
Sbjct: 121 FTTKKKQVFIDSAIKVMFVGSQILTQLVEDGNSMDDIREVLEKSDPLWESVNSKDSQGQT 180

Query: 181 LLKPAISSGKLTLVSALIDAGVDVNDKDSLKRSMMSLAVRTGKIDIVKRLIDSHCQVDFS 240
           LL  AIS  +  LV  +++   D+   +S+  + +  A  +G+  IV+ L+      + S
Sbjct: 181 LLHLAISKTRPDLVQLILEFKPDIEAINSVGSTPLEAASSSGESLIVELLLAHKANTEGS 240

Query: 241 VDLV---LHISAAMNRIDLIELLHRKFLEIPVNSVDSDGRTPIHTAAAHGHVEAIRFLIS 300
              V   +H ++    ++++ LL  K     V+S+  DG T +H A      +  R L++
Sbjct: 241 ESSVFRPIHHASREGHMEILRLLLLKGAR--VDSLTKDGNTSLHLAVEEKRRDCARLLLA 300

Query: 301 IGGDPTAVDW-EKWTPLHSAAAEGHFEAVEYLLSCSNVKYAVNSDGRTAFALAAENGHAD 360
            G      +  E  TPLH AAA G    V+ LL     KY  N  G+TAF +AAENGH+ 
Sbjct: 301 NGARTDVRNMREGDTPLHIAAANGDENMVKLLLHKGATKYVRNKLGKTAFDVAAENGHSR 360

Query: 361 LFDLLRLGDALHRTARAGDVRGLRSCVAAGANVNGKDQNGWTALHRAAFKGRVECIKALL 420
           LFD LRLGD L   AR G+VR ++  + +G  +NG+DQNGWT+LHRAAFKGR++ ++ L+
Sbjct: 361 LFDALRLGDNLCAAARKGEVRTIQKVLESGGVINGRDQNGWTSLHRAAFKGRMDAVRFLV 420

Query: 421 EHGADADAVDDAGYTPLRCAVEAGQEEVARLLLASGA 434
           E G D DA D+ GYT L CA E+G  +V   L+  GA
Sbjct: 421 EKGIDLDAKDEDGYTALHCAAESGHADVTEFLVKKGA 454

BLAST of Spg020093 vs. ExPASy Swiss-Prot
Match: Q9ULJ7 (Ankyrin repeat domain-containing protein 50 OS=Homo sapiens OX=9606 GN=ANKRD50 PE=1 SV=4)

HSP 1 Score: 136.7 bits (343), Expect = 6.5e-31
Identity = 107/350 (30.57%), Postives = 172/350 (49.14%), Query Frame = 0

Query: 115  KDASLLISFIGH-DVVEFLISHHNRIPDLSFLLNKAISGCTTSQLAALLKPAISSGKLTL 174
            + A +  +++GH ++VE L+ H   +       ++ + G T   +AAL  PA S G  ++
Sbjct: 678  RTALIAAAYMGHREIVEHLLDHGAEVN------HEDVDGRTALSVAALCVPA-SKGHASV 737

Query: 175  VSALIDAGVDVNDKDSLKRSMMSLAVRTGKIDIVKRLIDSHCQVDFSVD---LVLHISAA 234
            VS LID G +V+  D    + + +A   G +D+V  L++    VD + +     L  +A+
Sbjct: 738  VSLLIDRGAEVDHCDKDGMTPLLVAAYEGHVDVVDLLLEGGADVDHTDNNGRTPLLAAAS 797

Query: 235  MNRIDLIELLHRKFLEIPVNSVDSDGRTPIHTAAAHGHVEAIRFLISIGGDPTAVDWEKW 294
            M    ++  L   F    V+S+DS+GRT +  A+A G+VE +R L+  G D    D   W
Sbjct: 798  MGHASVVNTL--LFWGAAVDSIDSEGRTVLSIASAQGNVEVVRTLLDRGLDENHRDDAGW 857

Query: 295  TPLHSAAAEGHFEAVEYLLSCSNVKYAVNSDGRTAFALAAENGHADLFDLL--------R 354
            TPLH AA EGH    E L+        +++DGR  F LA++ GH D   +L        +
Sbjct: 858  TPLHMAAFEGHRLICEALIEQGARTNEIDNDGRIPFILASQEGHYDCVQILLENKSNIDQ 917

Query: 355  LGDALHRTARAGDVRGLRSCV----AAGANVNGKDQNGWTALHRAAFKGRVECIKALLEH 414
             G       R   + G R  V    + GA+VN KD +G   L+  A + ++   +  LE+
Sbjct: 918  RGYDGRNALRVAALEGHRDIVELLFSHGADVNCKDADGRPTLYILALENQLTMAEYFLEN 977

Query: 415  GADADAVDDAGYTPLRCAVEAGQEEVARLLLASGAKSNSLRGLKRGLIVS 449
            GA+ +A D  G T L  +   G  E+ ++L+A  A  N+    KR  + S
Sbjct: 978  GANVEASDAEGRTALHVSCWQGHMEMVQVLIAYHADVNAADNEKRSALQS 1018

BLAST of Spg020093 vs. ExPASy Swiss-Prot
Match: P16157 (Ankyrin-1 OS=Homo sapiens OX=9606 GN=ANK1 PE=1 SV=3)

HSP 1 Score: 123.6 bits (309), Expect = 5.7e-27
Identity = 88/309 (28.48%), Postives = 142/309 (45.95%), Query Frame = 0

Query: 145 LLNKAISGCTTSQLAALLKPAISSGKLTLVSALIDAGVDVNDKDSLKRSMMSLAVRTGKI 204
           L NKA            L  A   G   +V  L++   + N   +   + + +A R G +
Sbjct: 457 LQNKAKVNAKAKDDQTPLHCAARIGHTNMVKLLLENNANPNLATTAGHTPLHIAAREGHV 516

Query: 205 DIVKRLID---SHCQVDFSVDLVLHISAAMNRIDLIELLHRKFLEIPVNSVDSDGRTPIH 264
           + V  L++   S   +       LH++A   ++ + ELL  +  +   N+   +G TP+H
Sbjct: 517 ETVLALLEKEASQACMTKKGFTPLHVAAKYGKVRVAELLLER--DAHPNAAGKNGLTPLH 576

Query: 265 TAAAHGHVEAIRFLISIGGDPTAVDWEKWTPLHSAAAEGHFEAVEYLLSCSNVKYAVNSD 324
            A  H +++ ++ L+  GG P +  W  +TPLH AA +   E    LL       A +  
Sbjct: 577 VAVHHNNLDIVKLLLPRGGSPHSPAWNGYTPLHIAAKQNQVEVARSLLQYGGSANAESVQ 636

Query: 325 GRTAFALAAENGHADLFDLL-------RLGD-----ALHRTARAGDVRGLRSCVAAGANV 384
           G T   LAA+ GHA++  LL        LG+      LH  A+ G V      +  G  V
Sbjct: 637 GVTPLHLAAQEGHAEMVALLLSKQANGNLGNKSGLTPLHLVAQEGHVPVADVLIKHGVMV 696

Query: 385 NGKDQNGWTALHRAAFKGRVECIKALLEHGADADAVDDAGYTPLRCAVEAGQEEVARLLL 439
           +   + G+T LH A+  G ++ +K LL+H AD +A    GY+PL  A + G  ++  LLL
Sbjct: 697 DATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLL 756

BLAST of Spg020093 vs. ExPASy TrEMBL
Match: A0A6J1HB40 (ankyrin repeat domain-containing protein 50-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111461195 PE=4 SV=1)

HSP 1 Score: 769.6 bits (1986), Expect = 7.3e-219
Identity = 390/459 (84.97%), Postives = 423/459 (92.16%), Query Frame = 0

Query: 1   MDKLVRPDVKEVEFSFTKGEKCTVTFSLANLMHTMSVAVCLSTSNPSVFSFSQNFSIIPP 60
           MD+LV+PDVKEVEF FTKGEKC+ +F+LANLMHTMSVAVCL++SNPSVFSFSQ+FS+IPP
Sbjct: 1   MDRLVKPDVKEVEFFFTKGEKCSASFTLANLMHTMSVAVCLTSSNPSVFSFSQDFSVIPP 60

Query: 61  LSSSSYTISCKSLDQPPLSTPPDKLSVRSAMLPIGKAHTDDLRRLFSKPGRHIFKDASLL 120
           LSSSSYTISCKS DQPPLS P DK+SVRSAM+PIG AHTDDLR LFSKPGRHIFKDA LL
Sbjct: 61  LSSSSYTISCKSSDQPPLSNPSDKISVRSAMVPIGNAHTDDLRLLFSKPGRHIFKDALLL 120

Query: 121 ISFIGHDVVEFLISHHNRIPDLSFLLNKAISGCTTSQLAALLKPAISSGKLTLVSALIDA 180
           ISF+G DVVEFLISHHNRI +LSFLLNKAIS CT SQL AL++PAI SGKL LVSALIDA
Sbjct: 121 ISFVGPDVVEFLISHHNRITELSFLLNKAISSCTKSQLTALMEPAILSGKLGLVSALIDA 180

Query: 181 GVDVNDKDSLKRSMMSLAVRTGKIDIVKRLIDSHCQVDFSVDLVLHISAAMNRIDLIELL 240
           GVDVN KDSLKRSMMSLAVRTGKIDIVKRLIDSHCQVDFSVDLVLHI+A+MNR+D +ELL
Sbjct: 181 GVDVNVKDSLKRSMMSLAVRTGKIDIVKRLIDSHCQVDFSVDLVLHIAASMNRVDFMELL 240

Query: 241 HRKFLEIPVNSVDSDGRTPIHTAAAHGHVEAIRFLISIGGDPTAVDWEKWTPLHSAAAEG 300
            + F +IPVNSVDS+GRTPIHTAAA GHVEAIRFL+SIGG+P AVD  KWTPLHSAAAEG
Sbjct: 241 LKNFPDIPVNSVDSNGRTPIHTAAARGHVEAIRFLLSIGGNPEAVDRNKWTPLHSAAAEG 300

Query: 301 HFEAVEYLLSCSNVKYAVNSDGRTAFALAAENGHADLFDLLRLGDALHRTARAGDVRGLR 360
           HFEAVEY L+CSNVKYAVNSDG+TAFALA+ENGH DLFD LRLGDALHR ARAGDVRGLR
Sbjct: 301 HFEAVEYFLNCSNVKYAVNSDGKTAFALASENGHTDLFDSLRLGDALHRAARAGDVRGLR 360

Query: 361 SCVAAGANVNGKDQNGWTALHRAAFKGRVECIKALLEHGADADAVDDAGYTPLRCAVEAG 420
           SCVAAGA +NGKDQNGWTALHRAAFKGRVEC+KALLEHGAD DAVDDAGYTPLRCAVEAG
Sbjct: 361 SCVAAGAKINGKDQNGWTALHRAAFKGRVECVKALLEHGADTDAVDDAGYTPLRCAVEAG 420

Query: 421 QEEVARLLLASGAKSNSLRGLKRGLIVSGRQISSLCYGN 460
           QEEVAR+LLASGA+ N L+ L+R L VSGRQISSLC GN
Sbjct: 421 QEEVARVLLASGAEPNPLKCLRRDLAVSGRQISSLCCGN 459

BLAST of Spg020093 vs. ExPASy TrEMBL
Match: A0A6J1KSC8 (ankyrin repeat domain-containing protein 50-like OS=Cucurbita maxima OX=3661 GN=LOC111497807 PE=4 SV=1)

HSP 1 Score: 758.1 bits (1956), Expect = 2.2e-215
Identity = 384/459 (83.66%), Postives = 420/459 (91.50%), Query Frame = 0

Query: 1   MDKLVRPDVKEVEFSFTKGEKCTVTFSLANLMHTMSVAVCLSTSNPSVFSFSQNFSIIPP 60
           MD+LV+PDVKEVEF FTKGEKC+ TF+LANLMHTMSVAVCL++SNPSVFSFSQ+FS+IPP
Sbjct: 1   MDRLVKPDVKEVEFCFTKGEKCSATFTLANLMHTMSVAVCLTSSNPSVFSFSQDFSVIPP 60

Query: 61  LSSSSYTISCKSLDQPPLSTPPDKLSVRSAMLPIGKAHTDDLRRLFSKPGRHIFKDASLL 120
           LSSSSYTISCKS DQPPLS P DK+SVRSAM+PIG +HTDDLR LFSK GRHIFKDA LL
Sbjct: 61  LSSSSYTISCKSSDQPPLSNPSDKISVRSAMVPIGNSHTDDLRLLFSKHGRHIFKDALLL 120

Query: 121 ISFIGHDVVEFLISHHNRIPDLSFLLNKAISGCTTSQLAALLKPAISSGKLTLVSALIDA 180
           ISF+G DVVEFLISHHNRI +LSFL NKAIS CT SQL AL++PAI SGKL LVSALIDA
Sbjct: 121 ISFVGPDVVEFLISHHNRITELSFLFNKAISSCTKSQLTALMEPAILSGKLGLVSALIDA 180

Query: 181 GVDVNDKDSLKRSMMSLAVRTGKIDIVKRLIDSHCQVDFSVDLVLHISAAMNRIDLIELL 240
           GVDVN KDSLKRSMMSLAV TGKIDIVKRLIDSHCQ+DFSVDLVLHI+A+MNR+D +ELL
Sbjct: 181 GVDVNVKDSLKRSMMSLAVMTGKIDIVKRLIDSHCQIDFSVDLVLHIAASMNRVDFMELL 240

Query: 241 HRKFLEIPVNSVDSDGRTPIHTAAAHGHVEAIRFLISIGGDPTAVDWEKWTPLHSAAAEG 300
            +KF +I VNSVDS+GRTPIHTAAA GHVEAIRFL+SIGG+P AVD  KWTPLHSAAAEG
Sbjct: 241 VKKFPDILVNSVDSNGRTPIHTAAARGHVEAIRFLLSIGGNPEAVDRNKWTPLHSAAAEG 300

Query: 301 HFEAVEYLLSCSNVKYAVNSDGRTAFALAAENGHADLFDLLRLGDALHRTARAGDVRGLR 360
           HFEAVEY L+CSNVKYAVNSDG+TAFALA+ENGH DLFD LRLGDALHR ARAGDVRGLR
Sbjct: 301 HFEAVEYFLNCSNVKYAVNSDGKTAFALASENGHTDLFDSLRLGDALHRAARAGDVRGLR 360

Query: 361 SCVAAGANVNGKDQNGWTALHRAAFKGRVECIKALLEHGADADAVDDAGYTPLRCAVEAG 420
           SCVAAGA +NGKDQNGWTALHRAAFKGRVEC+KALLEHGAD DAVDDAGYTPL+CAVE+G
Sbjct: 361 SCVAAGAKINGKDQNGWTALHRAAFKGRVECVKALLEHGADTDAVDDAGYTPLQCAVESG 420

Query: 421 QEEVARLLLASGAKSNSLRGLKRGLIVSGRQISSLCYGN 460
           QEEVAR+LLASGA+ N L+ L+R L VSGRQISSLC GN
Sbjct: 421 QEEVARVLLASGAEPNPLKCLRRDLAVSGRQISSLCCGN 459

BLAST of Spg020093 vs. ExPASy TrEMBL
Match: A0A0A0KAL1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G390740 PE=4 SV=1)

HSP 1 Score: 723.0 bits (1865), Expect = 7.8e-205
Identity = 369/434 (85.02%), Postives = 394/434 (90.78%), Query Frame = 0

Query: 1   MDKLVRPDVKEVEFSFTKGEKCTVTFSLANLMHTMSVAVCLSTSNPSVFSFSQNFSIIPP 60
           MD+LV+PDV EVEFSF KGE CT TF L NLMHTMSVAVCLSTSNPSVFSFSQ+FSIIPP
Sbjct: 1   MDRLVKPDVDEVEFSFMKGENCTATFCLTNLMHTMSVAVCLSTSNPSVFSFSQDFSIIPP 60

Query: 61  LSSSSYTISCKSLDQPPLSTPPDKLSVRSAMLPIGKAHTDDLRRLFSKPGRHIFKDASLL 120
           LSSSSYTISCKS D+ PLSTPPDK+SVRSAMLPIGKAHTDDLRRLFSKPGRH+FKDASLL
Sbjct: 61  LSSSSYTISCKSSDKLPLSTPPDKISVRSAMLPIGKAHTDDLRRLFSKPGRHVFKDASLL 120

Query: 121 ISFIGHDVVEFLISHHNRIPDLSFLLNKAISGCTTSQLAALLKPAISSGKLTLVSALIDA 180
           ISF+G DVVEFLIS+H RIPDL  LLNKAISGC+ SQL AL++PA+SSGKL LVS LIDA
Sbjct: 121 ISFVGFDVVEFLISNHKRIPDLRSLLNKAISGCSKSQLTALMEPAVSSGKLGLVSVLIDA 180

Query: 181 GVDVNDKDSLKRSMMSLAVRTGKIDIVKRLIDSHCQVDFSVDLVLHISAAMNRIDLIELL 240
           GVDVN KD LK+SM+S AVRTGKIDIVKRLIDSHC++DFSVDLVLHI+AAMN +DLIELL
Sbjct: 181 GVDVNVKDCLKQSMLSSAVRTGKIDIVKRLIDSHCKIDFSVDLVLHIAAAMNHVDLIELL 240

Query: 241 HRKFLEIPVNSVDSDGRTPIHTAAAHGHVEAIRFLISIGGDPTAVDWEKWTPLHSAAAEG 300
              F +IPVNSVDSDGRTPIHTAAAHGHVE I FL S+GGD  AVD  KWTPLH AAA G
Sbjct: 241 RENFPDIPVNSVDSDGRTPIHTAAAHGHVEVISFLASVGGDVEAVDRTKWTPLHFAAAGG 300

Query: 301 HFEAVEYLLSCSNVKYAVNSDGRTAFALAAENGHADLFDLLRLGDALHRTARAGDVRGLR 360
           H EAVEYLL+CSNVKYAVNSDGRTAFALA+ENGH DLFD LRL DALHRTARAGDVRGLR
Sbjct: 301 HLEAVEYLLNCSNVKYAVNSDGRTAFALASENGHTDLFDSLRLDDALHRTARAGDVRGLR 360

Query: 361 SCVAAGANVNGKDQNGWTALHRAAFKGRVECIKALLEHGADADAVDDAGYTPLRCAVEAG 420
           SCVAAGA VNGKDQNGWTALHRAAFKGRVEC+KALLE GA+ADAVD+AGYTPLRCAVEAG
Sbjct: 361 SCVAAGAKVNGKDQNGWTALHRAAFKGRVECVKALLEVGAEADAVDNAGYTPLRCAVEAG 420

Query: 421 QEEVARLLLASGAK 435
           QEEVARLLL SGAK
Sbjct: 421 QEEVARLLLDSGAK 434

BLAST of Spg020093 vs. ExPASy TrEMBL
Match: A0A6J1H957 (ankyrin repeat domain-containing protein 50-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111461195 PE=4 SV=1)

HSP 1 Score: 719.5 bits (1856), Expect = 8.6e-204
Identity = 366/428 (85.51%), Postives = 394/428 (92.06%), Query Frame = 0

Query: 32  MHTMSVAVCLSTSNPSVFSFSQNFSIIPPLSSSSYTISCKSLDQPPLSTPPDKLSVRSAM 91
           MHTMSVAVCL++SNPSVFSFSQ+FS+IPPLSSSSYTISCKS DQPPLS P DK+SVRSAM
Sbjct: 1   MHTMSVAVCLTSSNPSVFSFSQDFSVIPPLSSSSYTISCKSSDQPPLSNPSDKISVRSAM 60

Query: 92  LPIGKAHTDDLRRLFSKPGRHIFKDASLLISFIGHDVVEFLISHHNRIPDLSFLLNKAIS 151
           +PIG AHTDDLR LFSKPGRHIFKDA LLISF+G DVVEFLISHHNRI +LSFLLNKAIS
Sbjct: 61  VPIGNAHTDDLRLLFSKPGRHIFKDALLLISFVGPDVVEFLISHHNRITELSFLLNKAIS 120

Query: 152 GCTTSQLAALLKPAISSGKLTLVSALIDAGVDVNDKDSLKRSMMSLAVRTGKIDIVKRLI 211
            CT SQL AL++PAI SGKL LVSALIDAGVDVN KDSLKRSMMSLAVRTGKIDIVKRLI
Sbjct: 121 SCTKSQLTALMEPAILSGKLGLVSALIDAGVDVNVKDSLKRSMMSLAVRTGKIDIVKRLI 180

Query: 212 DSHCQVDFSVDLVLHISAAMNRIDLIELLHRKFLEIPVNSVDSDGRTPIHTAAAHGHVEA 271
           DSHCQVDFSVDLVLHI+A+MNR+D +ELL + F +IPVNSVDS+GRTPIHTAAA GHVEA
Sbjct: 181 DSHCQVDFSVDLVLHIAASMNRVDFMELLLKNFPDIPVNSVDSNGRTPIHTAAARGHVEA 240

Query: 272 IRFLISIGGDPTAVDWEKWTPLHSAAAEGHFEAVEYLLSCSNVKYAVNSDGRTAFALAAE 331
           IRFL+SIGG+P AVD  KWTPLHSAAAEGHFEAVEY L+CSNVKYAVNSDG+TAFALA+E
Sbjct: 241 IRFLLSIGGNPEAVDRNKWTPLHSAAAEGHFEAVEYFLNCSNVKYAVNSDGKTAFALASE 300

Query: 332 NGHADLFDLLRLGDALHRTARAGDVRGLRSCVAAGANVNGKDQNGWTALHRAAFKGRVEC 391
           NGH DLFD LRLGDALHR ARAGDVRGLRSCVAAGA +NGKDQNGWTALHRAAFKGRVEC
Sbjct: 301 NGHTDLFDSLRLGDALHRAARAGDVRGLRSCVAAGAKINGKDQNGWTALHRAAFKGRVEC 360

Query: 392 IKALLEHGADADAVDDAGYTPLRCAVEAGQEEVARLLLASGAKSNSLRGLKRGLIVSGRQ 451
           +KALLEHGAD DAVDDAGYTPLRCAVEAGQEEVAR+LLASGA+ N L+ L+R L VSGRQ
Sbjct: 361 VKALLEHGADTDAVDDAGYTPLRCAVEAGQEEVARVLLASGAEPNPLKCLRRDLAVSGRQ 420

Query: 452 ISSLCYGN 460
           ISSLC GN
Sbjct: 421 ISSLCCGN 428

BLAST of Spg020093 vs. ExPASy TrEMBL
Match: A0A5D3D412 (Tankyrase-2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G002150 PE=4 SV=1)

HSP 1 Score: 718.4 bits (1853), Expect = 1.9e-203
Identity = 365/434 (84.10%), Postives = 394/434 (90.78%), Query Frame = 0

Query: 1   MDKLVRPDVKEVEFSFTKGEKCTVTFSLANLMHTMSVAVCLSTSNPSVFSFSQNFSIIPP 60
           MD+LV+PDVKEVEFSF KGE CT TF L NLMHTMSVAVCLSTSNPSVFSFSQ+FSIIPP
Sbjct: 1   MDRLVKPDVKEVEFSFMKGENCTATFCLTNLMHTMSVAVCLSTSNPSVFSFSQDFSIIPP 60

Query: 61  LSSSSYTISCKSLDQPPLSTPPDKLSVRSAMLPIGKAHTDDLRRLFSKPGRHIFKDASLL 120
           LSSSSYTISCKS D+ PLSTPPDK+SVRSAMLPIGKAHTDDLRRLFSKPGRH+FKDASLL
Sbjct: 61  LSSSSYTISCKSSDKLPLSTPPDKISVRSAMLPIGKAHTDDLRRLFSKPGRHVFKDASLL 120

Query: 121 ISFIGHDVVEFLISHHNRIPDLSFLLNKAISGCTTSQLAALLKPAISSGKLTLVSALIDA 180
           ISF+G DVVE+LIS+H RIPDL  LLNKAISGC+ SQL AL++PA+SSGKL LVSALIDA
Sbjct: 121 ISFVGFDVVEYLISNHKRIPDLRSLLNKAISGCSKSQLTALMEPAVSSGKLGLVSALIDA 180

Query: 181 GVDVNDKDSLKRSMMSLAVRTGKIDIVKRLIDSHCQVDFSVDLVLHISAAMNRIDLIELL 240
           GVDVN  +SLK+SM+S AVR GKIDIVKRLIDSHC++DFSVDLVLHI+AAMNR+DLIELL
Sbjct: 181 GVDVNVNNSLKQSMLSTAVRIGKIDIVKRLIDSHCKIDFSVDLVLHIAAAMNRVDLIELL 240

Query: 241 HRKFLEIPVNSVDSDGRTPIHTAAAHGHVEAIRFLISIGGDPTAVDWEKWTPLHSAAAEG 300
              F +IPVNSVDSDGRTPIHTAAAHGHVE I FL S+GGD   VD  KWTPLH AAA G
Sbjct: 241 RENFPDIPVNSVDSDGRTPIHTAAAHGHVEVISFLASVGGDVETVDRTKWTPLHFAAAGG 300

Query: 301 HFEAVEYLLSCSNVKYAVNSDGRTAFALAAENGHADLFDLLRLGDALHRTARAGDVRGLR 360
           H E V+YLL+CSNVKYAVNS+GRTAFALA+ENGH DLFDLLRL DALHRTARAGDVRGLR
Sbjct: 301 HLETVDYLLNCSNVKYAVNSEGRTAFALASENGHTDLFDLLRLDDALHRTARAGDVRGLR 360

Query: 361 SCVAAGANVNGKDQNGWTALHRAAFKGRVECIKALLEHGADADAVDDAGYTPLRCAVEAG 420
           SCVAAGA VNGKDQNGWTALHRAAFKGRVEC+KALLE GA+ADAVD+AGYTPLRCAVEAG
Sbjct: 361 SCVAAGAKVNGKDQNGWTALHRAAFKGRVECVKALLEVGAEADAVDNAGYTPLRCAVEAG 420

Query: 421 QEEVARLLLASGAK 435
            EEVARLLL SGAK
Sbjct: 421 HEEVARLLLDSGAK 434

BLAST of Spg020093 vs. TAIR 10
Match: AT2G03430.1 (Ankyrin repeat family protein )

HSP 1 Score: 102.4 bits (254), Expect = 9.6e-22
Identity = 70/197 (35.53%), Postives = 98/197 (49.75%), Query Frame = 0

Query: 240 LHRKFLEIPVNSVDSDGRTPIHTAAAHGHVEAIRFLISIGGDPTAV---DWEKWTPLHSA 299
           L  + L   +N  + DGR+ +H AA+ GH + ++ L S     T +   D E W PLHSA
Sbjct: 31  LSEEQLSKSLNFRNEDGRSLLHVAASFGHSQIVKLLSSSDEAKTVINSKDDEGWAPLHSA 90

Query: 300 AAEGHFEAVEYLLSCSNVKYAVNSDGRTAFALAAENGHADLFDLLRLGDALHRTARAGDV 359
           A+ G+ E VE LL+      A N+ GRTA   AA  G  ++  LL               
Sbjct: 91  ASIGNAELVEVLLTRGADVNAKNNGGRTALHYAASKGRLEIAQLL--------------- 150

Query: 360 RGLRSCVAAGANVNGKDQNGWTALHRAAFKGRVECIKALLEHGADADAVDDAGYTPLRCA 419
                 +  GA +N  D+ G T LHRAA  G++E  + L+E GA+ DA D  G T L  +
Sbjct: 151 ------LTHGAKINITDKVGCTPLHRAASVGKLEVCEFLIEEGAEIDATDKMGQTALMHS 206

Query: 420 VEAGQEEVARLLLASGA 434
           V    ++VA LL+  GA
Sbjct: 211 VICDDKQVAFLLIRHGA 206

BLAST of Spg020093 vs. TAIR 10
Match: AT5G60070.1 (ankyrin repeat family protein )

HSP 1 Score: 97.1 bits (240), Expect = 4.0e-20
Identity = 80/274 (29.20%), Postives = 126/274 (45.99%), Query Frame = 0

Query: 193 SMMSLAVRTGKIDIVKRLIDSHCQVDFSV-----------DLVLHISAAMNRIDLIELLH 252
           S +  AVR G    VK ++ +H + +  +           +  L+++A     D++  L 
Sbjct: 34  SQLLSAVRRGDFSAVKEILSNHMESEDELRDLLRKQNQCGETALYVAAEYGDADVVAELI 93

Query: 253 RKFLEIPVNSVDSDGRTPIHTAAAHGHVEAIRFLISIGGD-PTAVDWEKWTPLHSAAAEG 312
           + +      +   +G  P H AA  G ++ +R L+    +    VD    T LH+AAA+G
Sbjct: 94  KYYDLEDAETKARNGFDPFHIAAKQGELDVLRVLMEEHPELSMTVDLSNTTALHTAAAQG 153

Query: 313 HFEAVEYLLSC--SNVKYAVNSDGRTAFALAAENGHADLFDLLRL--GDALHRTARAGD- 372
           H E VEYLL    S++     S+G+TA   AA NGHA++   +     D   RT + G  
Sbjct: 154 HVEVVEYLLEAAGSSLAAIAKSNGKTALHSAARNGHAEVVKAIVAVEPDTATRTDKKGQT 213

Query: 373 -----VRGLRSCVAA------GANVNGKDQNGWTALHRAAFKGRVECIKALL---EHGAD 432
                V+G    V         +++N  D  G TALH A  KGR++ ++ LL   E    
Sbjct: 214 PLHMAVKGQSIDVVVELMKGHRSSLNMADSKGNTALHVATRKGRIKIVELLLDNNETSPS 273

Query: 433 ADAVDDAGYTPLRCAVEAGQEEVARLLLASGAKS 436
             A++ AG TPL  A + G  ++A +L   G  S
Sbjct: 274 TKAINRAGETPLDTAEKTGHPQIAAVLKTRGVPS 307

BLAST of Spg020093 vs. TAIR 10
Match: AT1G07710.1 (Ankyrin repeat family protein )

HSP 1 Score: 90.1 bits (222), Expect = 4.9e-18
Identity = 72/248 (29.03%), Postives = 116/248 (46.77%), Query Frame = 0

Query: 197 LAVRTGKIDIVKRLIDSHCQVDFSVDL-----VLHISAAMNRIDLIELLHRKFLEIPVNS 256
           +A   G ++IVK +I+ +      +         HI+A    +D++++L     E+ + +
Sbjct: 72  VAAEYGDVEIVKEMINCYDLALVEIKARNGFDAFHIAAKQGDLDVLKVLAEAHSELAM-T 131

Query: 257 VDSDGRTPIHTAAAHGHVEAIRFLISIGGDPTAVDWEKW-TPLHSAAAEGHFEAVEYLLS 316
           VD    T +HTAA  GH E + FL+ +G     +      T LHSA+  GH + ++ LL+
Sbjct: 132 VDLSNTTALHTAATQGHTEVVNFLLELGSSLAGIAKSNGKTALHSASRNGHVKVIKALLA 191

Query: 317 CSNVKYAVNSD--GRTAFALAAENGHADLFDLLRLGDALHRTARAGDVRGLRSCVAAGAN 376
            S    A+  D  G+TA  +A +  + ++ + L   D                     ++
Sbjct: 192 -SEPAIAIRMDKKGQTALHMAVKGTNVEVVEELIKADR--------------------SS 251

Query: 377 VNGKDQNGWTALHRAAFKGRVECIKALLEHG-ADADAVDDAGYTPLRCAVEAGQEEVARL 436
           +N  D  G TALH AA KGR + +K LL +   D  AV+ +G T L  A + G  EVA +
Sbjct: 252 INIADTKGNTALHIAARKGRSQIVKLLLANNMTDTKAVNRSGETALDTAEKIGNPEVALI 297

BLAST of Spg020093 vs. TAIR 10
Match: AT2G26650.1 (K+ transporter 1 )

HSP 1 Score: 86.3 bits (212), Expect = 7.1e-17
Identity = 63/208 (30.29%), Postives = 93/208 (44.71%), Query Frame = 0

Query: 221 VDLVLHISAAMNRIDLIELLHRKFLE-IPVNSVDSDGRTPIHTAAAHGHVEAIRFLISIG 280
           +DL L++  A  R D + LLH+     +  N  D++GRTP+H AA+ G +  +  L+   
Sbjct: 516 MDLPLNLCFAAIREDDL-LLHQLLKRGLDPNESDNNGRTPLHIAASKGTLNCVLLLLEYH 575

Query: 281 GDPTAVDWEKWTPLHSAAAEGHFEAVEYLLSCSNVKYAVNSDGRTAFALAAENGHADLFD 340
            DP   D E   PL  A  EGH + V+ LL                        H    D
Sbjct: 576 ADPNCRDAEGSVPLWEAMVEGHEKVVKVLLE-----------------------HGSTID 635

Query: 341 LLRLGDALHRTARAGDVRGLRSCVAAGANVNGKDQNGWTALHRAAFKGRVECIKALLEHG 400
              +G      A  G+++ L+  V  G +V      G +ALH A  +  +E +K LLE G
Sbjct: 636 AGDVGHFACTAAEQGNLKLLKEIVLHGGDVTRPRATGTSALHTAVCEENIEMVKYLLEQG 695

Query: 401 ADADAVDDAGYTPLRCAVEAGQEEVARL 428
           AD +  D  G+TP   A + G E++  L
Sbjct: 696 ADVNKQDMHGWTPRDLAEQQGHEDIKAL 699

BLAST of Spg020093 vs. TAIR 10
Match: AT2G25600.1 (Shaker pollen inward K+ channel )

HSP 1 Score: 81.3 bits (199), Expect = 2.3e-15
Identity = 69/215 (32.09%), Postives = 94/215 (43.72%), Query Frame = 0

Query: 214 HCQVDFSVDLVLHISAAMNRIDLIELLHRKFLE-IPVNSVDSDGRTPIHTAAAHGHVEAI 273
           H      +DL L +  A  R D + LLH+        N +D DGRT +H AA+ G    +
Sbjct: 535 HMLAQGKMDLPLSLCFAAARGDDL-LLHQLLRRGSSPNEMDKDGRTALHIAASKGSHYCV 594

Query: 274 RFLISIGGDPTAVDWEKWTPLHSAAAEGHFEAVEYLLSCSNVKYAVNSDGRTAFALAAEN 333
             L+  G DP   D E   PL  A    H E +  LL+ +  K +++S    +  LA E 
Sbjct: 595 VLLLEHGADPNIRDSEGNVPLWEAIIGRHRE-IAKLLAENGAKLSLDSVSYFS-GLAVEK 654

Query: 334 GHADLFDLLRLGDALHRTARAGDVRGLRSCVAAGANVNGKDQNGWTALHRAAFKGRVECI 393
              D                      L+  +  G +V   D NG TALHRA  +G +E +
Sbjct: 655 NCLD---------------------ALKDIIKYGGDVTLPDGNGTTALHRAVSEGHLEIV 714

Query: 394 KALLEHGADADAVDDAGYTPLRCAVEAGQEEVARL 428
           K LL+ GAD D  D  G+TP   A   G EE+  L
Sbjct: 715 KFLLDQGADLDWPDSYGWTPRGLADHQGNEEIKTL 725