Spg018061 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg018061
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	rRNA N-glycosidase
Location	scaffold9: 29683043 .. 29685106 (+)
RNA-Seq Expression	Spg018061
Synteny	Spg018061

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGTGCATTAACTCAAGTACTCAACACTTGATACGACCCCCCGATTGCGGTTTTCATGCTCCCGATCCTATCCGCGCTCGTTTCGTCGCCAATCCTAATATTAGCTTCTCTCTATTGCATGCCACTGGCACAACATACAAACAATTCATAAACGATCTGCGTAGGGCACTCACAGTGAACTCTCACCTAGTGTACGGCATCCCTGTGTTGCCAAACCCGACAGGGCTAGCACGCTTCATATTCGTCAATCTCACCAACTACAAAGGTGAATCCATCACTGTGGCAATCAAGGTAGAAGATGCTTACATCGTGGCATACCAAGCAGGAAACAGGGCCTACTTTCTAAACGATGTTTACGACGAACTAAAACAAGTGCTATTTAAAGGTAGCGAAAGTGGAAAACTCACTTTAATGGGAATTATGATGACCTCGAGAGGTGTGACGCCCGAAGTTAGGAGGGGTACATGAATGAACCGAGATCACATCCGAATGAGAGGGATCCTGAGGACATGAAAGTATGGTCAAGATAAGCTTAAAAGAATTGATAGATACTACCTATACCAACAAGGTGCACCTTCCTTTTCGGTGGCTCAATCATAGGAACTTTGAAGTTAAGCGTGCTTAGCTTGGAGAAGTTCTATGTTGGGTGACCTACTGGGAATTTTCCTAGGAAGCGTGTGAGTGAGGACAAAGCATGCTAAAAGGACCACGTGTTGGTTTGTGGGGACAGTATTCAATCTTAGAAGTAGTATCAGACAAGTATGGTAGCGTGGCCAGGTCGCAGGGGAATGTCGGGGCCGTAAGCATGCTAAAAAGACCCCGTGTTGGTTTGTGGGGATAGTCTTCACTCTTGGAAGCAGTTTAAGACAAGTATGGTAACGTCACCAGGTCGCAGGGGAATAGCGGGGCCTTAGGTGCCAAATCCGAATTCCGAATCCTGGGCCTGGGTCGTCGTTACAGATGGTATCAGAGCGGAACCTCTCCCAGTAGGATGTGGTTCGGGGACGAACCAAAGCGGAAGCTGGTGGGCATGTGACGCCCGAAGTTAGGAGGGGTACATGAATGAACCGAGATCACATCCGAATGAGAGGGATCCTGAGGACATGAAAGTATGGTCAAGATAAGCTTAAAAGAATTGATAGATACTACCTATACCAACAAGGTGCACCTTCCTTTTCGGTGGCTCAATCATAGGAACTTTGAAGTTAAGCGTGCTTGGCTTGGAGAAGTTCTATGTTGGGTGACCTCCTAGGAATTTTCCTAGGAAGCATGTGAGTGAGGACAAAGCATGCTAAAAGGACCCCGTGTTGGTTTGTGGGGATAGTCTTCACTCTTGGAATAGCGGGGCCTTAGGTGCCAAATCCGAATTCCGAATCCTGGGCCTAGGTCGTTACAAGAGGAGTGCAGGATCGAGAGAGAAGATTGATCTTGGATTCTCCGAATTAGACAGTGCCATTGGCAATCTGTTCCACAGTAAAAATGGTAATTCTGTTGCCAGAGGATTCATTGTGATCATTCAAACGATTTCAAAAGCCGCGAGGTTTAGGTATATTGAGCAAAGAGTTTCTGTGAATGTCGAGTCAAAGTTTCTGCCAGATCCGGCATTCTTGAGCTTAGAAAACCGTTGGAAGGATCTCTGTAAACAGATACTGTTGGCGCAAAAGCACGATGGGAAATTTGAGCGTCCAGTTCCAGTTCGAAGTGTTTCCAACAAACCAATTCTGGTAAAAGATGTTAATTCACCAGTTATCAAAGGCATTGCACTGTTACGCAACACGTCCAAAGAGGATGGTCGACCAATCCATATCCATCTGATGGAACCGTGGTTTGATCATTGGGAAGATGCTCGAGGTGACTTTGGAATAGATTTCTAAAGAAATTGTGCGCTTTTTGTATAAATATATATTGTAATAAATAAGTGTGGAATATTTATCTTGCCTTGGTATAATATCTAAATAAGTTCTTCTATCACGTCAAAGCTTCCATCTCTTATATAGAATTGAGAAGAAAACGAAAATTATAAATTCTTTATATTTTTACTATTTTTTAAACACAGTTGGT

mRNA sequence

ATGGAGTGCATTAACTCAAGTACTCAACACTTGATACGACCCCCCGATTGCGGTTTTCATGCTCCCGATCCTATCCGCGCTCGTTTCGTCGCCAATCCTAATATTAGCTTCTCTCTATTGCATGCCACTGGCACAACATACAAACAATTCATAAACGATCTGCGTAGGGCACTCACAGTGAACTCTCACCTAGTGTACGGCATCCCTGTGTTGCCAAACCCGACAGGGCTAGCACGCTTCATATTCGTCAATCTCACCAACTACAAAGGTGAATCCATCACTGTGGCAATCAAGGTAGAAGATGCTTACATCGTGGCATACCAAGCAGGAAACAGGGCCTACTTTCTAAACGATGTTTACGACGAACTAAAACAAGTGCTATTTAAAGATGGTATCAGAGCGGAACCTCTCCCAAGGAGTGCAGGATCGAGAGAGAAGATTGATCTTGGATTCTCCGAATTAGACAGTGCCATTGGCAATCTGTTCCACAGTAAAAATGGTAATTCTGTTGCCAGAGGATTCATTGTGATCATTCAAACGATTTCAAAAGCCGCGAGGTTTAGGTATATTGAGCAAAGAGTTTCTGTGAATGTCGAGTCAAAGTTTCTGCCAGATCCGGCATTCTTGAGCTTAGAAAACCGTTGGAAGGATCTCTGTAAACAGATACTGTTGGCGCAAAAGCACGATGGGAAATTTGAGCGTCCAGTTCCAGTTCGAAGTGTTTCCAACAAACCAATTCTGGTAAAAGATGTTAATTCACCAGTTATCAAAGGCATTGCACTGTTACGCAACACGTCCAAAGAGGATGGTCGACCAATCCATATCCATCTGATGGAACCGTGGTTTGATCATTGGGAAGATGCTCGAGGTGACTTTGGAATAGATTTCTAA

Coding sequence (CDS)

ATGGAGTGCATTAACTCAAGTACTCAACACTTGATACGACCCCCCGATTGCGGTTTTCATGCTCCCGATCCTATCCGCGCTCGTTTCGTCGCCAATCCTAATATTAGCTTCTCTCTATTGCATGCCACTGGCACAACATACAAACAATTCATAAACGATCTGCGTAGGGCACTCACAGTGAACTCTCACCTAGTGTACGGCATCCCTGTGTTGCCAAACCCGACAGGGCTAGCACGCTTCATATTCGTCAATCTCACCAACTACAAAGGTGAATCCATCACTGTGGCAATCAAGGTAGAAGATGCTTACATCGTGGCATACCAAGCAGGAAACAGGGCCTACTTTCTAAACGATGTTTACGACGAACTAAAACAAGTGCTATTTAAAGATGGTATCAGAGCGGAACCTCTCCCAAGGAGTGCAGGATCGAGAGAGAAGATTGATCTTGGATTCTCCGAATTAGACAGTGCCATTGGCAATCTGTTCCACAGTAAAAATGGTAATTCTGTTGCCAGAGGATTCATTGTGATCATTCAAACGATTTCAAAAGCCGCGAGGTTTAGGTATATTGAGCAAAGAGTTTCTGTGAATGTCGAGTCAAAGTTTCTGCCAGATCCGGCATTCTTGAGCTTAGAAAACCGTTGGAAGGATCTCTGTAAACAGATACTGTTGGCGCAAAAGCACGATGGGAAATTTGAGCGTCCAGTTCCAGTTCGAAGTGTTTCCAACAAACCAATTCTGGTAAAAGATGTTAATTCACCAGTTATCAAAGGCATTGCACTGTTACGCAACACGTCCAAAGAGGATGGTCGACCAATCCATATCCATCTGATGGAACCGTGGTTTGATCATTGGGAAGATGCTCGAGGTGACTTTGGAATAGATTTCTAA

Protein sequence

MECINSSTQHLIRPPDCGFHAPDPIRARFVANPNISFSLLHATGTTYKQFINDLRRALTVNSHLVYGIPVLPNPTGLARFIFVNLTNYKGESITVAIKVEDAYIVAYQAGNRAYFLNDVYDELKQVLFKDGIRAEPLPRSAGSREKIDLGFSELDSAIGNLFHSKNGNSVARGFIVIIQTISKAARFRYIEQRVSVNVESKFLPDPAFLSLENRWKDLCKQILLAQKHDGKFERPVPVRSVSNKPILVKDVNSPVIKGIALLRNTSKEDGRPIHIHLMEPWFDHWEDARGDFGIDF

Homology

[Close]

BLAST of Spg018061 vs. NCBI nr
Match: XP_022926255.1 (ribosome-inactivating protein bryodin II-like [Cucurbita moschata])

HSP 1 Score: 338.2 bits (866), Expect = 7.2e-89
Identity = 198/311 (63.67%), Postives = 219/311 (70.42%), Query Frame = 0


Query: 1   MECINSSTQHLIRPP---DCGFHAPDPIRA-RFVANPNISFSLLHATGTTYKQFINDLRR 60
           MECI  STQ LI+ P   +CG HA   IRA R  AN N+SFSLL AT  TYKQFINDLR+
Sbjct: 1   MECI-GSTQDLIQAPFPLNCGAHA--AIRAPRPSANANLSFSLLGATAQTYKQFINDLRK 60

Query: 61  ALTVNSHLVYGIPVLP-NPTGLARFIFVNLTNYKGESITVAIKVEDAYIVAYQAGNRAYF 120
           ALTVNSH VY IPVLP    GLARFI VNLTNYKGESITVAI   D YIVAYQAGN+AYF
Sbjct: 61  ALTVNSHKVYDIPVLPATAKGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNKAYF 120

Query: 121 LNDVYDELKQVLFKDGIRA--------EPLPRSAG--SREKIDLGFSELDSAIGNLFHSK 180
           L DV  E   VLFK             + L R AG  SRE I+LGFSEL S+IGN+FH K
Sbjct: 121 LKDVSKEAHDVLFKGSKHETLSYKGNYDDLERHAGKISRENIELGFSELSSSIGNMFHYK 180

Query: 181 NGNSVARGFIVIIQTISKAARFRYIEQRVSVNVESKFLPDPAFLSLENRWKDLCKQILLA 240
            G SV R FIV+IQ +S+A+RF+YIE R    VESKF PDP +LSLENRW +L +Q+  A
Sbjct: 181 AGYSVPRSFIVMIQMLSEASRFKYIETRNCEKVESKFKPDPGYLSLENRWSNLSEQVQKA 240

Query: 241 QKHDGKFERPVPVRSVSNKPILVKDVNSPVIKGIALLRNTSKEDGRPIHIHLMEPWFDHW 297
           QKH GKFE PV VRSVSNKP+LV DVNSPV+KGIALL   S + G      LME  F HW
Sbjct: 241 QKHGGKFEHPVQVRSVSNKPVLVSDVNSPVVKGIALL--LSNKPGVNGVSKLMEE-FIHW 300

[Close]

BLAST of Spg018061 vs. NCBI nr
Match: XP_023523582.1 (ribosome-inactivating protein bryodin II-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 333.2 bits (853), Expect = 2.3e-87
Identity = 197/311 (63.34%), Postives = 216/311 (69.45%), Query Frame = 0


Query: 1   MECINSSTQHLIRPP---DCGFHAPDPIRA-RFVANPNISFSLLHATGTTYKQFINDLRR 60
           MECI  STQ LI+ P   +CG HA   IRA R  AN N+SFSLL AT  TYKQFINDLR 
Sbjct: 1   MECI-GSTQDLIQAPFPLNCGAHA--AIRAPRPSANANLSFSLLGATAQTYKQFINDLRN 60

Query: 61  ALTVNSHLVYGIPVLP-NPTGLARFIFVNLTNYKGESITVAIKVEDAYIVAYQAGNRAYF 120
           ALTV SH VYGIPVLP    GLARFI VNLTNYKGESITVAI   D YIVAYQAGN+AYF
Sbjct: 61  ALTVQSHKVYGIPVLPATAKGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNKAYF 120

Query: 121 LNDVYDELKQVLFKDGIRA--------EPLPRSAG--SREKIDLGFSELDSAIGNLFHSK 180
           L DV  E   VLFK             + L R AG  SRE I+LGFSEL S+IGN+FH  
Sbjct: 121 LKDVSKEAHDVLFKGSKHETLSYKGNYDDLERHAGMISRENIELGFSELSSSIGNMFHYN 180

Query: 181 NGNSVARGFIVIIQTISKAARFRYIEQRVSVNVESKFLPDPAFLSLENRWKDLCKQILLA 240
            G SV R FIV+IQ +S+A+RF+YIE R    VESKF PDP +LSLENRW +L +Q+  A
Sbjct: 181 AGYSVPRSFIVMIQMLSEASRFKYIETRNCEKVESKFKPDPGYLSLENRWSNLSEQVQKA 240

Query: 241 QKHDGKFERPVPVRSVSNKPILVKDVNSPVIKGIALLRNTSKEDGRPIHIHLMEPWFDHW 297
           QKH GKFERPV VRSVSNKPI V DVNSPV+KGIALL     + G      LME  F HW
Sbjct: 241 QKHGGKFERPVQVRSVSNKPIKVTDVNSPVVKGIALL--LCNKPGVNGVSKLMEQ-FIHW 300

[Close]

BLAST of Spg018061 vs. NCBI nr
Match: KAG7037232.1 (hypothetical protein SDJN02_00855, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 328.6 bits (841), Expect = 5.7e-86
Identity = 194/311 (62.38%), Postives = 216/311 (69.45%), Query Frame = 0


Query: 1   MECINSSTQHLIRPP---DCGFHAPDPIRA-RFVANPNISFSLLHATGTTYKQFINDLRR 60
           MECI  STQ LI+ P   +CG HA   IRA R  AN N+SFSLL AT  TYKQFINDLR+
Sbjct: 1   MECI-GSTQDLIQAPFPLNCGAHA--AIRAPRPSANANLSFSLLGATAQTYKQFINDLRK 60

Query: 61  ALTVNSHLVYGIPVLP-NPTGLARFIFVNLTNYKGESITVAIKVEDAYIVAYQAGNRAYF 120
           ALTVNSH VY IPVLP    GLARFI VNLTNYKGESITVAI   D YIVAYQAGN+AYF
Sbjct: 61  ALTVNSHKVYDIPVLPATAKGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNKAYF 120

Query: 121 LNDVYDELKQVLFKDGIRA--------EPLPRSAG--SREKIDLGFSELDSAIGNLFHSK 180
           L DV  E   VLFK             + L R AG  SRE I+LGFSEL S+IGN+FH  
Sbjct: 121 LKDVSKEAHDVLFKGSKHETLSYKGNYDDLERHAGKISRENIELGFSELSSSIGNMFHYN 180

Query: 181 NGNSVARGFIVIIQTISKAARFRYIEQRVSVNVESKFLPDPAFLSLENRWKDLCKQILLA 240
            G SV R FIV+IQ +S+A+RF+YIE R    VESKF PDP +LSLENRW +L +Q+  A
Sbjct: 181 AGYSVPRSFIVMIQMLSEASRFKYIETRNCEKVESKFKPDPGYLSLENRWSNLSEQVQKA 240

Query: 241 QKHDGKFERPVPVRSVSNKPILVKDVNSPVIKGIALLRNTSKEDGRPIHIHLMEPWFDHW 297
           QKH GKFE PV VRSVSNKP+LV DVNSPV+KGIALL     + G      LME  F  W
Sbjct: 241 QKHGGKFEHPVQVRSVSNKPVLVSDVNSPVVKGIALL--LCNKPGVNGVSKLMEE-FIKW 300

[Close]

BLAST of Spg018061 vs. NCBI nr
Match: XP_022981562.1 (ribosome-inactivating protein bryodin II-like [Cucurbita maxima])

HSP 1 Score: 327.4 bits (838), Expect = 1.3e-85
Identity = 192/311 (61.74%), Postives = 216/311 (69.45%), Query Frame = 0


Query: 1   MECINSSTQHLIRPP---DCGFHAPDPIRA-RFVANPNISFSLLHATGTTYKQFINDLRR 60
           MECI  ST+ LI+ P   +CG H    IRA R  AN N+SFSLL AT  TYKQFI+DLR+
Sbjct: 1   MECI-GSTKDLIQAPFPLNCGAHV--AIRAPRPSANANLSFSLLGATAQTYKQFISDLRK 60

Query: 61  ALTVNSHLVYGIPVLP-NPTGLARFIFVNLTNYKGESITVAIKVEDAYIVAYQAGNRAYF 120
           ALTVNSH VY IPVLP    GLARFI VNLTNYKGESITVAI   D YIVAYQAGN+AYF
Sbjct: 61  ALTVNSHKVYDIPVLPATAKGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNKAYF 120

Query: 121 LNDVYDELKQVLFKDGIRA--------EPLPRSAG--SREKIDLGFSELDSAIGNLFHSK 180
           L DV  E   VLFK             + L R AG  SRE IDLGFSEL S+IGN+FH  
Sbjct: 121 LKDVSKEAHDVLFKGSKHETLSYKGNYDDLERHAGKISRENIDLGFSELSSSIGNMFHYN 180

Query: 181 NGNSVARGFIVIIQTISKAARFRYIEQRVSVNVESKFLPDPAFLSLENRWKDLCKQILLA 240
            G SV + FIV+IQ +S+A+RF+YIE R   NVESKF PDP +LSLENRW +L +Q+  A
Sbjct: 181 AGYSVPKSFIVMIQMLSEASRFKYIEARNCENVESKFKPDPGYLSLENRWSNLSEQVQKA 240

Query: 241 QKHDGKFERPVPVRSVSNKPILVKDVNSPVIKGIALLRNTSKEDGRPIHIHLMEPWFDHW 297
           QKH GKFE PV VRSVSNKP+LV DVNSPV+KG+ALL     + G      LME  F  W
Sbjct: 241 QKHGGKFEHPVQVRSVSNKPVLVSDVNSPVVKGMALL--LCNKPGVNGVSKLMEE-FIKW 300

[Close]

BLAST of Spg018061 vs. NCBI nr
Match: KAG7037234.1 (hypothetical protein SDJN02_00857, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 321.6 bits (823), Expect = 7.0e-84
Identity = 188/310 (60.65%), Postives = 210/310 (67.74%), Query Frame = 0


Query: 1   MECINSSTQHLIRPP---DCGFHAPDPIRARFVANPNISFSLLHATGTTYKQFINDLRRA 60
           MECI  STQ LI+ P   +CG HA   +  R  AN N+SFSLL AT  TYKQFI DLR+A
Sbjct: 1   MECI-GSTQDLIQAPFPLNCGAHAAIRV-PRPSANANVSFSLLGATAETYKQFIKDLRKA 60

Query: 61  LTVNSHLVYGIPVLP-NPTGLARFIFVNLTNYKGESITVAIKVEDAYIVAYQAGNRAYFL 120
           LTVNSH VYGIPVLP    GLARFI VNLTNY GESITVAI   D YIVAYQAGN+AYFL
Sbjct: 61  LTVNSHKVYGIPVLPATAKGLARFILVNLTNYNGESITVAIDTVDVYIVAYQAGNKAYFL 120

Query: 121 NDVYDELKQVLFKDGIRA--------EPLPRSAG--SREKIDLGFSELDSAIGNLFHSKN 180
            DV  E   VLFK             + L R AG  SRE + LGFSEL S+IGN+FH   
Sbjct: 121 KDVSKEAHNVLFKGSKHETLWYKGNYDDLERHAGKISRENVQLGFSELSSSIGNMFHYNA 180

Query: 181 GNSVARGFIVIIQTISKAARFRYIEQRVSVNVESKFLPDPAFLSLENRWKDLCKQILLAQ 240
           G SV R FIV+IQ +S+A+RF+YIE+  S NV S F PDP +LSLENRW +L +Q+  AQ
Sbjct: 181 GYSVPRSFIVMIQMLSEASRFKYIEKINSDNVSSTFKPDPGYLSLENRWNNLSEQVQKAQ 240

Query: 241 KHDGKFERPVPVRSVSNKPILVKDVNSPVIKGIALLRNTSKEDGRPIHIHLMEPWFDHWE 297
           KH GKFE  V VRSV NK I V DVNSPV+KGIALL     + G      LME  F HWE
Sbjct: 241 KHGGKFEHAVQVRSVGNKSIKVTDVNSPVVKGIALL--LCNKPGANGVSKLMEE-FIHWE 300

[Close]

BLAST of Spg018061 vs. ExPASy Swiss-Prot
Match: P98184 (Ribosome-inactivating protein bryodin II OS=Bryonia dioica OX=3652 PE=1 SV=1)

HSP 1 Score: 255.0 bits (650), Expect = 1.1e-66
Identity = 141/249 (56.63%), Postives = 170/249 (68.27%), Query Frame = 0


Query: 25  IRARFVANPNISFSLLHATGTTYKQFINDLRRALTVNSHLVYGIPVLPN-PTGLARFIFV 84
           + A    + +I+FSL+ ATG TYK FI +LR  LTV +  VY IPVL N   GLARF  V
Sbjct: 14  VGAHVTEDVDINFSLIGATGATYKTFIRNLRTKLTVGTPRVYDIPVLRNAAAGLARFQLV 73

Query: 85  NLTNYKGESITVAIKVEDAYIVAYQAGNRAYFLNDVYDELKQVLFKD--------GIRAE 144
            LTNY GES+TVA+ V + Y+VAY+AGN AYFL D   E   VLF          G   +
Sbjct: 74  TLTNYNGESVTVALDVVNVYVVAYRAGNTAYFLADASTEANNVLFAGINHVRLPYGGNYD 133

Query: 145 PLPRSAG--SREKIDLGFSELDSAIGNLFHSKNGNSVARGFIVIIQTISKAARFRYIEQR 204
            L  +AG  SRE I+LGFSE+ SAIGN+F    G SV R FIVIIQT+S+AARF+YIEQR
Sbjct: 134 GLETAAGRISRENIELGFSEISSAIGNMFRHNPGTSVPRAFIVIIQTVSEAARFKYIEQR 193

Query: 205 VSVNVESKFLPDPAFLSLENRWKDLCKQILLAQKHDGKFERPVPVRSVSNKPILVKDVNS 263
           VS NV +KF PDPAFLSL+N W  L +QI +AQ   G+F RPV +R+VSN P  V +VNS
Sbjct: 194 VSENVGTKFKPDPAFLSLQNAWGSLSEQIQIAQTRGGEFARPVELRTVSNTPTFVTNVNS 253

[Close]

BLAST of Spg018061 vs. ExPASy Swiss-Prot
Match: P09989 (Ribosome-inactivating protein alpha-trichosanthin OS=Trichosanthes kirilowii OX=3677 PE=1 SV=2)

HSP 1 Score: 167.5 bits (423), Expect = 2.2e-40
Identity = 101/244 (41.39%), Postives = 150/244 (61.48%), Query Frame = 0


Query: 34  NISFSLLHATGTTYKQFINDLRRALTVNSHLVYGIPVLPNP-TGLARFIFVNLTNYKGES 93
           ++SF L  AT ++Y  FI++LR+AL  N   +Y IP+L +   G  R+  ++LTNY  E+
Sbjct: 24  DVSFRLSGATSSSYGVFISNLRKALP-NERKLYDIPLLRSSLPGSQRYALIHLTNYADET 83

Query: 94  ITVAIKVEDAYIVAYQAGNRAYFLNDV-YDELKQVLFKDGIRAEPLPRS---------AG 153
           I+VAI V + YI+ Y+AG+ +YF N+    E  + +FKD +R   LP S         AG
Sbjct: 84  ISVAIDVTNVYIMGYRAGDTSYFFNEASATEAAKYVFKDAMRKVTLPYSGNYERLQTAAG 143

Query: 154 S-REKIDLGFSELDSAIGNLFHSKNGNSVARGFIVIIQTISKAARFRYIEQRVSVNVESK 213
             RE I LG   LDSAI  LF+  N NS A   +V+IQ+ S+AAR+++IEQ++   V+  
Sbjct: 144 KIRENIPLGLPALDSAITTLFY-YNANSAASALMVLIQSTSEAARYKFIEQQIGKRVDKT 203

Query: 214 FLPDPAFLSLENRWKDLCKQILLAQKHDGKFERPVPVRSVSNKPILVKDVNSPVI-KGIA 265
           FLP  A +SLEN W  L KQI +A  ++G+FE PV + +  N+ + + +V++ V+   IA
Sbjct: 204 FLPSLAIISLENSWSALSKQIQIASTNNGQFESPVVLINAQNQRVTITNVDAGVVTSNIA 263

[Close]

BLAST of Spg018061 vs. ExPASy Swiss-Prot
Match: P24478 (Ribosome-inactivating protein karasurin-C OS=Trichosanthes kirilowii OX=3677 PE=1 SV=2)

HSP 1 Score: 164.1 bits (414), Expect = 2.4e-39
Identity = 100/244 (40.98%), Postives = 148/244 (60.66%), Query Frame = 0


Query: 34  NISFSLLHATGTTYKQFINDLRRALTVNSHLVYGIPVLPNP-TGLARFIFVNLTNYKGES 93
           ++SF L  AT ++Y  FI++LR+AL     L Y IP+L +   G  R+  ++LTNY  E+
Sbjct: 24  DVSFRLSGATSSSYGVFISNLRKALPYERKL-YDIPLLRSTLPGSQRYALIHLTNYADET 83

Query: 94  ITVAIKVEDAYIVAYQAGNRAYFLNDV-YDELKQVLFKDGIRAEPLPRS---------AG 153
           I+VAI V + Y++ Y+AG+ +YF N+    E  + +FKD  R   LP S         AG
Sbjct: 84  ISVAIDVTNVYVMGYRAGDTSYFFNEASATEAAKYVFKDAKRKVTLPYSGNYERLQIAAG 143

Query: 154 S-REKIDLGFSELDSAIGNLFHSKNGNSVARGFIVIIQTISKAARFRYIEQRVSVNVESK 213
             RE I LG   LDSAI  LF+  N NS A   +V+IQ+ S+AAR+++IEQ++   V+  
Sbjct: 144 KIRENIPLGLPALDSAITTLFY-YNANSAASALMVLIQSTSEAARYKFIEQQIGKRVDKT 203

Query: 214 FLPDPAFLSLENRWKDLCKQILLAQKHDGKFERPVPVRSVSNKPILVKDVNSPVI-KGIA 265
           FLP  A +SLEN W  L KQI +A  ++G+FE PV + +  N+ + + +V++ V+   IA
Sbjct: 204 FLPSLAIISLENSWSALSKQIQIASTNNGQFETPVVLINAQNQRVTITNVDAGVVTSNIA 263

[Close]

BLAST of Spg018061 vs. ExPASy Swiss-Prot
Match: P84531 (Ribosome-inactivating protein cucurmosin OS=Cucurbita moschata OX=3662 PE=1 SV=2)

HSP 1 Score: 164.1 bits (414), Expect = 2.4e-39
Identity = 102/243 (41.98%), Postives = 138/243 (56.79%), Query Frame = 0


Query: 34  NISFSLLHATGTTYKQFINDLRRALTVNSHLVYGIPVLPNPT-GLARFIFVNLTNYKGES 93
           N+ F L  AT ++YK FI +LR AL  +   VY IPVL +      RFI ++L NY G+S
Sbjct: 1   NVRFDLSSATSSSYKTFIKNLREALPKDGK-VYDIPVLLSTVMDSRRFILIDLVNYDGQS 60

Query: 94  ITVAIKVEDAYIVAYQAGNRAYFLNDVYDELKQVLFKDGIRAEPLPRSAG---------- 153
           IT AI V + YIVAY  G  +YF   V  +  ++LFK G +   LP +            
Sbjct: 61  ITAAIDVLNVYIVAYSTGTVSYFFQQVPAQAPKLLFK-GTQQRTLPYTGNYENLQTAAKK 120

Query: 154 SREKIDLGFSELDSAIGNLFHSKNGNSVARGFIVIIQTISKAARFRYIEQRVSVNVESKF 213
            RE I+LG   LDSAI  LFH  N  + A   +V+IQT S+AARFRYIE +++ NV +KF
Sbjct: 121 LRENIELGLPALDSAITTLFH-YNAEAAASALLVLIQTTSEAARFRYIELQIANNVGTKF 180

Query: 214 LPDPAFLSLENRWKDLCKQILLAQKHDGKFERPVPVRSVSNKPILVKDVNSPVI-KGIAL 265
            P    +SLEN W  L KQI +A+  +G+FE PV +       + + +V S V+ + I L
Sbjct: 181 KPSQTIISLENNWSALSKQIQIAKNKNGQFETPVILIDPQGNRVQITNVTSNVVTQNIQL 240

[Close]

BLAST of Spg018061 vs. ExPASy Swiss-Prot
Match: P24817 (Ribosome-inactivating protein beta-momorcharin OS=Momordica charantia OX=3673 GN=MAP30 PE=1 SV=2)

HSP 1 Score: 161.4 bits (407), Expect = 1.6e-38
Identity = 103/247 (41.70%), Postives = 141/247 (57.09%), Query Frame = 0


Query: 31  ANPNISFSLLHATGTTYKQFINDLRRALTVNSHLVYGIPVLPNP-TGLARFIFVNLTNYK 90
           A  +++F L  AT  TY +FI D R  L   SH VY IP+L +  +   RFI +NLT+Y 
Sbjct: 21  AKGDVNFDLSTATAKTYTKFIEDFRATLPF-SHKVYDIPLLYSTISDSRRFILLNLTSYA 80

Query: 91  GESITVAIKVEDAYIVAYQAGNRAYFLNDVYDELKQVLFKDGIRAEPLPRSAG------- 150
            E+I+VAI V + Y+VAY+  + +YF  +   E   +LFK G R   LP +         
Sbjct: 81  YETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFK-GTRKITLPYTGNYENLQTA 140

Query: 151 ---SREKIDLGFSELDSAIGNLFHSKNGNSVARGFIVIIQTISKAARFRYIEQRVSVNVE 210
               RE IDLG   L SAI  LF+  N  S     +V+IQT ++AARF+YIE+ V+  V 
Sbjct: 141 AHKIRENIDLGLPALSSAITTLFY-YNAQSAPSALLVLIQTTAEAARFKYIERHVAKYVA 200

Query: 211 SKFLPDPAFLSLENRWKDLCKQILLAQKHDGKFERPVPVRSVSNKPILVKDVNSPVIKG- 266
           + F P+ A +SLEN+W  L KQI LAQ   GKF  PV +   + +   V +V+S V+KG 
Sbjct: 201 TNFKPNLAIISLENQWSALSKQIFLAQNQGGKFRNPVDLIKPTGERFQVTNVDSDVVKGN 260

[Close]

BLAST of Spg018061 vs. ExPASy TrEMBL
Match: A0A6J1EKL0 (rRNA N-glycosidase OS=Cucurbita moschata OX=3662 GN=LOC111433434 PE=3 SV=1)

HSP 1 Score: 338.2 bits (866), Expect = 3.5e-89
Identity = 198/311 (63.67%), Postives = 219/311 (70.42%), Query Frame = 0


Query: 1   MECINSSTQHLIRPP---DCGFHAPDPIRA-RFVANPNISFSLLHATGTTYKQFINDLRR 60
           MECI  STQ LI+ P   +CG HA   IRA R  AN N+SFSLL AT  TYKQFINDLR+
Sbjct: 1   MECI-GSTQDLIQAPFPLNCGAHA--AIRAPRPSANANLSFSLLGATAQTYKQFINDLRK 60

Query: 61  ALTVNSHLVYGIPVLP-NPTGLARFIFVNLTNYKGESITVAIKVEDAYIVAYQAGNRAYF 120
           ALTVNSH VY IPVLP    GLARFI VNLTNYKGESITVAI   D YIVAYQAGN+AYF
Sbjct: 61  ALTVNSHKVYDIPVLPATAKGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNKAYF 120

Query: 121 LNDVYDELKQVLFKDGIRA--------EPLPRSAG--SREKIDLGFSELDSAIGNLFHSK 180
           L DV  E   VLFK             + L R AG  SRE I+LGFSEL S+IGN+FH K
Sbjct: 121 LKDVSKEAHDVLFKGSKHETLSYKGNYDDLERHAGKISRENIELGFSELSSSIGNMFHYK 180

Query: 181 NGNSVARGFIVIIQTISKAARFRYIEQRVSVNVESKFLPDPAFLSLENRWKDLCKQILLA 240
            G SV R FIV+IQ +S+A+RF+YIE R    VESKF PDP +LSLENRW +L +Q+  A
Sbjct: 181 AGYSVPRSFIVMIQMLSEASRFKYIETRNCEKVESKFKPDPGYLSLENRWSNLSEQVQKA 240

Query: 241 QKHDGKFERPVPVRSVSNKPILVKDVNSPVIKGIALLRNTSKEDGRPIHIHLMEPWFDHW 297
           QKH GKFE PV VRSVSNKP+LV DVNSPV+KGIALL   S + G      LME  F HW
Sbjct: 241 QKHGGKFEHPVQVRSVSNKPVLVSDVNSPVVKGIALL--LSNKPGVNGVSKLMEE-FIHW 300

[Close]

BLAST of Spg018061 vs. ExPASy TrEMBL
Match: A0A6J1IUB2 (rRNA N-glycosidase OS=Cucurbita maxima OX=3661 GN=LOC111480641 PE=3 SV=1)

HSP 1 Score: 327.4 bits (838), Expect = 6.2e-86
Identity = 192/311 (61.74%), Postives = 216/311 (69.45%), Query Frame = 0


Query: 1   MECINSSTQHLIRPP---DCGFHAPDPIRA-RFVANPNISFSLLHATGTTYKQFINDLRR 60
           MECI  ST+ LI+ P   +CG H    IRA R  AN N+SFSLL AT  TYKQFI+DLR+
Sbjct: 1   MECI-GSTKDLIQAPFPLNCGAHV--AIRAPRPSANANLSFSLLGATAQTYKQFISDLRK 60

Query: 61  ALTVNSHLVYGIPVLP-NPTGLARFIFVNLTNYKGESITVAIKVEDAYIVAYQAGNRAYF 120
           ALTVNSH VY IPVLP    GLARFI VNLTNYKGESITVAI   D YIVAYQAGN+AYF
Sbjct: 61  ALTVNSHKVYDIPVLPATAKGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNKAYF 120

Query: 121 LNDVYDELKQVLFKDGIRA--------EPLPRSAG--SREKIDLGFSELDSAIGNLFHSK 180
           L DV  E   VLFK             + L R AG  SRE IDLGFSEL S+IGN+FH  
Sbjct: 121 LKDVSKEAHDVLFKGSKHETLSYKGNYDDLERHAGKISRENIDLGFSELSSSIGNMFHYN 180

Query: 181 NGNSVARGFIVIIQTISKAARFRYIEQRVSVNVESKFLPDPAFLSLENRWKDLCKQILLA 240
            G SV + FIV+IQ +S+A+RF+YIE R   NVESKF PDP +LSLENRW +L +Q+  A
Sbjct: 181 AGYSVPKSFIVMIQMLSEASRFKYIEARNCENVESKFKPDPGYLSLENRWSNLSEQVQKA 240

Query: 241 QKHDGKFERPVPVRSVSNKPILVKDVNSPVIKGIALLRNTSKEDGRPIHIHLMEPWFDHW 297
           QKH GKFE PV VRSVSNKP+LV DVNSPV+KG+ALL     + G      LME  F  W
Sbjct: 241 QKHGGKFEHPVQVRSVSNKPVLVSDVNSPVVKGMALL--LCNKPGVNGVSKLMEE-FIKW 300

[Close]

BLAST of Spg018061 vs. ExPASy TrEMBL
Match: A0A6J1E093 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024726 PE=3 SV=1)

HSP 1 Score: 314.3 bits (804), Expect = 5.4e-82
Identity = 177/310 (57.10%), Postives = 212/310 (68.39%), Query Frame = 0


Query: 1   MECINSSTQHLIRPP--DCGFHAPDPIRARFVANPNISFSLLHATGTTYKQFINDLRRAL 60
           MEC+ SS   +   P  D G H      A    N N+SFSLL ATG TY+QFI +LR AL
Sbjct: 1   MECVRSSEPFIQPLPFNDIGIHGVHANGAVNAPNSNVSFSLLGATGKTYQQFIQNLRNAL 60

Query: 61  TVNSHLVYGIPVL-PNPTGLARFIFVNLTNYKGESITVAIKVEDAYIVAYQAGNRAYFLN 120
           T+NS +VY IPVL       ARFI V+LTNYK E+ITVAI V + YIVAYQAGN+AYFL 
Sbjct: 61  TINSKIVYNIPVLAATALSSARFILVHLTNYKNETITVAIDVVNVYIVAYQAGNKAYFLK 120

Query: 121 DVYDELKQVLFKDGIRAEPLP---------RSAG--SREKIDLGFSELDSAIGNLFHSKN 180
           D   E + VLFK GI+ E LP          +AG  SREKIDLGFSEL SAIGN++H   
Sbjct: 121 DASKEARDVLFK-GIKQEILPYKGNYDGLETAAGKISREKIDLGFSELGSAIGNMYHYNA 180

Query: 181 GNSVARGFIVIIQTISKAARFRYIEQRVSVNVESKFLPDPAFLSLENRWKDLCKQILLAQ 240
           G SV R FIV+IQTIS+AARF+YIE +VS NVE+KF PDP FLSLENRW DL +Q+ +AQ
Sbjct: 181 GTSVPRAFIVMIQTISEAARFKYIETKVSQNVETKFKPDPGFLSLENRWSDLSEQVQIAQ 240

Query: 241 KHDGKFERPVPVRSVSNKPILVKDVNSPVIKGIALLRNTSKEDGRPIHIHLMEPWFDHWE 297
              G+F RP+ VRSV+NKPILV +V S V++G+ALL   SK  G    + LME +   WE
Sbjct: 241 NRKGEFARPIEVRSVTNKPILVTNVKSKVVEGMALLL-YSKPRGNGDLMELMEEFNKEWE 300

[Close]

BLAST of Spg018061 vs. ExPASy TrEMBL
Match: A0A6J1DWK4 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024728 PE=3 SV=1)

HSP 1 Score: 275.4 bits (703), Expect = 2.8e-70
Identity = 147/244 (60.25%), Postives = 183/244 (75.00%), Query Frame = 0


Query: 31  ANPNISFSLLHATGTTYKQFINDLRRALTVNSHLVYGIPVLPN-PTGLARFIFVNLTNYK 90
           A  ++SFS+L AT  TY+QFI +LR  LT+ S +VYGIPVL +   G ARFI V+LTNY 
Sbjct: 19  AEADVSFSMLGATSETYRQFIRNLRSTLTIRSPVVYGIPVLQSTAVGSARFILVHLTNYN 78

Query: 91  GESITVAIKVEDAYIVAYQAGNRAYFLNDVYDELKQVLFKDGIRAEPLP---------RS 150
            ESITVAI V + Y+VAY+AGN AYFLND   E   VLFK GI+   LP          +
Sbjct: 79  TESITVAIDVVNIYVVAYRAGNNAYFLNDASAEANNVLFK-GIKHVRLPYKGNYDGLETA 138

Query: 151 AG--SREKIDLGFSELDSAIGNLFHSKNGNSVARGFIVIIQTISKAARFRYIEQRVSVNV 210
           AG  SREKIDLGFSE+ S+IGN+FH   G S+AR FIVIIQ++ +AARF+YIEQRVS NV
Sbjct: 139 AGKISREKIDLGFSEISSSIGNMFHHNVGTSIARAFIVIIQSVLEAARFKYIEQRVSENV 198

Query: 211 ESKFLPDPAFLSLENRWKDLCKQILLAQKHDGKFERPVPVRSVSNKPILVKDVNSPVIKG 263
           ++KF PDPAFLSLENRW DL +QI +AQK +G+F R + +R+VSNKPI+V +V+SP++KG
Sbjct: 199 KTKFKPDPAFLSLENRWSDLSEQIQIAQKREGEFARAIELRTVSNKPIIVTNVSSPIVKG 258

[Close]

BLAST of Spg018061 vs. ExPASy TrEMBL
Match: A0A6J1DVE7 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024729 PE=3 SV=1)

HSP 1 Score: 269.2 bits (687), Expect = 2.0e-68
Identity = 145/243 (59.67%), Postives = 176/243 (72.43%), Query Frame = 0


Query: 31  ANPNISFSLLHATGTTYKQFINDLRRALTVNSHLVYGIPVL-PNPTGLARFIFVNLTNYK 90
           A  ++SFS+L +T  TYKQFI ++R ALTV S +VY IPVL    TG ARF+ V+LTNY 
Sbjct: 19  AEADVSFSMLGSTSETYKQFIKNVRSALTVGSQIVYDIPVLKATATGSARFLLVHLTNYN 78

Query: 91  GESITVAIKVEDAYIVAYQAGNRAYFLNDVYDELKQVLFKDGIRAEPLPRSAG------- 150
            ESI VAI V + Y+VAY+AGN AYFLND   E  +VLF+ GI    L            
Sbjct: 79  SESIIVAIDVVNVYVVAYRAGNNAYFLNDASAEADRVLFQ-GINHVKLSYKGNYDGLETA 138

Query: 151 ---SREKIDLGFSELDSAIGNLFHSKNGNSVARGFIVIIQTISKAARFRYIEQRVSVNVE 210
              SREKIDLGFSE+ S+IGN+FH   G SV R FIVIIQ++S+AARF+YIEQRVS NV+
Sbjct: 139 AKISREKIDLGFSEISSSIGNMFHHNAGTSVPRAFIVIIQSVSEAARFKYIEQRVSENVK 198

Query: 211 SKFLPDPAFLSLENRWKDLCKQILLAQKHDGKFERPVPVRSVSNKPILVKDVNSPVIKGI 263
           + F PDPAFLSL+NRW DL +QI +AQK  G+F R V +R+VSNKPILV DV+SPV+KGI
Sbjct: 199 TTFKPDPAFLSLQNRWSDLSEQIQIAQKRGGEFARAVELRTVSNKPILVTDVSSPVVKGI 258

The following BLAST results are available for this feature:

BLAST of Spg018061 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022926255.1	7.2e-89	63.67	ribosome-inactivating protein bryodin II-like [Cucurbita moschata]	[more]
XP_023523582.1	2.3e-87	63.34	ribosome-inactivating protein bryodin II-like [Cucurbita pepo subsp. pepo]	[more]
KAG7037232.1	5.7e-86	62.38	hypothetical protein SDJN02_00855, partial [Cucurbita argyrosperma subsp. argyro...	[more]
XP_022981562.1	1.3e-85	61.74	ribosome-inactivating protein bryodin II-like [Cucurbita maxima]	[more]
KAG7037234.1	7.0e-84	60.65	hypothetical protein SDJN02_00857, partial [Cucurbita argyrosperma subsp. argyro...	[more]

BLAST of Spg018061 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P98184	1.1e-66	56.63	Ribosome-inactivating protein bryodin II OS=Bryonia dioica OX=3652 PE=1 SV=1	[more]
P09989	2.2e-40	41.39	Ribosome-inactivating protein alpha-trichosanthin OS=Trichosanthes kirilowii OX=...	[more]
P24478	2.4e-39	40.98	Ribosome-inactivating protein karasurin-C OS=Trichosanthes kirilowii OX=3677 PE=...	[more]
P84531	2.4e-39	41.98	Ribosome-inactivating protein cucurmosin OS=Cucurbita moschata OX=3662 PE=1 SV=2	[more]
P24817	1.6e-38	41.70	Ribosome-inactivating protein beta-momorcharin OS=Momordica charantia OX=3673 GN...	[more]

BLAST of Spg018061 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EKL0	3.5e-89	63.67	rRNA N-glycosidase OS=Cucurbita moschata OX=3662 GN=LOC111433434 PE=3 SV=1	[more]
A0A6J1IUB2	6.2e-86	61.74	rRNA N-glycosidase OS=Cucurbita maxima OX=3661 GN=LOC111480641 PE=3 SV=1	[more]
A0A6J1E093	5.4e-82	57.10	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024726 PE=3 SV=1	[more]
A0A6J1DWK4	2.8e-70	60.25	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024728 PE=3 SV=1	[more]
A0A6J1DVE7	2.0e-68	59.67	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024729 PE=3 SV=1	[more]

BLAST of Spg018061 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR017989	Ribosome-inactivating protein type 1/2	PRINTS	PR00396	SHIGARICIN	coord: 206..223 score: 37.57 coord: 92..107 score: 43.59 coord: 174..194 score: 55.39 coord: 45..58 score: 40.6
IPR016139	Ribosome-inactivating protein, subdomain 2	GENE3D	4.10.470.10	Ricin (A Subunit), domain 2	coord: 186..267 e-value: 9.5E-21 score: 75.6
IPR016138	Ribosome-inactivating protein, subdomain 1	GENE3D	3.40.420.10	Ricin (A subunit), domain 1	coord: 32..185 e-value: 2.2E-32 score: 114.1
IPR001574	Ribosome-inactivating protein	PFAM	PF00161	RIP	coord: 45..223 e-value: 2.0E-25 score: 89.6
IPR001574	Ribosome-inactivating protein	PANTHER	PTHR33453	FAMILY NOT NAMED	coord: 34..272
None	No IPR available	PANTHER	PTHR33453:SF14	RRNA N-GLYCOSIDASE	coord: 34..272
IPR036041	Ribosome-inactivating protein superfamily	SUPERFAMILY	56371	Ribosome inactivating proteins (RIP)	coord: 34..266

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg018061.1	Spg018061.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0017148	negative regulation of translation
molecular_function	GO:0030598	rRNA N-glycosylase activity