Spg010650 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg010650
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	nicotianamine synthase-like
Location	scaffold2: 2252853 .. 2253413 (+)
RNA-Seq Expression	Spg010650
Synteny	Spg010650

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

atgcatcttaccctcttccatagacgtttccacattgccaccccatttacaagccattcgcgagtgcctcatggttctctgcggccgagccgagggcctactcgagttcgaattctcgaccttgctcggaaaaatccccaaacctctcaacaatctcactctatttccatattaccagaactacatcaattagcccaccttgaacacaagattttgaatgacaatggagttgtgaatccaaaaaaggtagcttttgtgggatcgggtcctttgcctctaacttccattatcatggccatgcagcacatgaagaccacgtatttcgataattacgacattgacagcatggcaaatgacgtggcgggaaagatcgtggcgtcggatgctgagctggagggaagaatgaagttttggacaagagatattgttgaggtaaaggaagagcttgggggatatgattgtgtgtttttggcagctcttgtagggatgaagaaggaagaaaaagtgaagattttagggcacttgagaaagtatatgaagaaggtgggattttggttgtga

mRNA sequence

atgcatcttaccctcttccatagacgtttccacattgccaccccatttacaagccattcgcgagtgcctcatggttctctgcggccgagccgagggcctactcgagttcgaattctcgaccttgctcggaaaaatccccaaacctctcaacaatctcactctatttccatattaccagaactacatcaattagcccaccttgaacacaagattttgaatgacaatggagttgtgaatccaaaaaaggtagcttttgtgggatcgggtcctttgcctctaacttccattatcatggccatgcagcacatgaagaccacgtatttcgataattacgacattgacagcatggcaaatgacgtggcgggaaagatcgtggcgtcggatgctgagctggagggaagaatgaagttttggacaagagatattgttgaggtaaaggaagagcttgggggatatgattgtgtgtttttggcagctcttgtagggatgaagaaggaagaaaaagtgaagattttagggcacttgagaaagtatatgaagaaggtgggattttggttgtga

Coding sequence (CDS)

atgcatcttaccctcttccatagacgtttccacattgccaccccatttacaagccattcgcgagtgcctcatggttctctgcggccgagccgagggcctactcgagttcgaattctcgaccttgctcggaaaaatccccaaacctctcaacaatctcactctatttccatattaccagaactacatcaattagcccaccttgaacacaagattttgaatgacaatggagttgtgaatccaaaaaaggtagcttttgtgggatcgggtcctttgcctctaacttccattatcatggccatgcagcacatgaagaccacgtatttcgataattacgacattgacagcatggcaaatgacgtggcgggaaagatcgtggcgtcggatgctgagctggagggaagaatgaagttttggacaagagatattgttgaggtaaaggaagagcttgggggatatgattgtgtgtttttggcagctcttgtagggatgaagaaggaagaaaaagtgaagattttagggcacttgagaaagtatatgaagaaggtgggattttggttgtga

Protein sequence

MHLTLFHRRFHIATPFTSHSRVPHGSLRPSRGPTRVRILDLARKNPQTSQQSHSISILPELHQLAHLEHKILNDNGVVNPKKVAFVGSGPLPLTSIIMAMQHMKTTYFDNYDIDSMANDVAGKIVASDAELEGRMKFWTRDIVEVKEELGGYDCVFLAALVGMKKEEKVKILGHLRKYMKKVGFWL

Homology

[Close]

BLAST of Spg010650 vs. NCBI nr
Match: XP_038880344.1 (nicotianamine synthase-like [Benincasa hispida])

HSP 1 Score: 220.3 bits (560), Expect = 1.4e-53
Identity = 107/121 (88.43%), Postives = 115/121 (95.04%), Query Frame = 0


Query: 63  QLAHLEHKILNDNGVVNPKKVAFVGSGPLPLTSIIMAMQHMKTTYFDNYDIDSMANDVAG 122
           +LA+LE+KILNDNG+ NPKKVAFVGSGPLPLTSIIMAMQHMK TYFDNYDIDS+ANDVAG
Sbjct: 114 KLANLENKILNDNGIFNPKKVAFVGSGPLPLTSIIMAMQHMKGTYFDNYDIDSVANDVAG 173

Query: 123 KIVASDAELEGRMKFWTRDIVEVKEELGGYDCVFLAALVGMKKEEKVKILGHLRKYMKKV 182
           KIV SD ELEGRMKFWTRDIVEVKEELGGYDCVFLAALVGMKKE+KVKI+ HLRKYMK+ 
Sbjct: 174 KIVGSDTELEGRMKFWTRDIVEVKEELGGYDCVFLAALVGMKKEDKVKIIRHLRKYMKEG 233

Query: 183 G 184
           G
Sbjct: 234 G 234

[Close]

BLAST of Spg010650 vs. NCBI nr
Match: XP_022987937.1 (nicotianamine synthase-like [Cucurbita maxima])

HSP 1 Score: 218.0 bits (554), Expect = 6.8e-53
Identity = 107/121 (88.43%), Postives = 114/121 (94.21%), Query Frame = 0


Query: 63  QLAHLEHKILNDNGVVNPKKVAFVGSGPLPLTSIIMAMQHMKTTYFDNYDIDSMANDVAG 122
           +LAHLE+KIL DNGVVNPKKVAFVGSGPLPLTSII+AMQHMK TYFDNYDIDSMAN+VAG
Sbjct: 114 KLAHLEYKILIDNGVVNPKKVAFVGSGPLPLTSIILAMQHMKNTYFDNYDIDSMANEVAG 173

Query: 123 KIVASDAELEGRMKFWTRDIVEVKEELGGYDCVFLAALVGMKKEEKVKILGHLRKYMKKV 182
           KIVASD ELEGRMKFWTRDIVEV EEL GYDCVFLAALVG+KKE+KVKIL HLRKYMK+ 
Sbjct: 174 KIVASDGELEGRMKFWTRDIVEVGEELEGYDCVFLAALVGLKKEDKVKILSHLRKYMKQG 233

Query: 183 G 184
           G
Sbjct: 234 G 234

[Close]

BLAST of Spg010650 vs. NCBI nr
Match: XP_023516742.1 (nicotianamine synthase-like [Cucurbita pepo subsp. pepo] >XP_023521836.1 nicotianamine synthase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 216.5 bits (550), Expect = 2.0e-52
Identity = 105/121 (86.78%), Postives = 114/121 (94.21%), Query Frame = 0


Query: 63  QLAHLEHKILNDNGVVNPKKVAFVGSGPLPLTSIIMAMQHMKTTYFDNYDIDSMANDVAG 122
           +LAHLE+KIL DNG+VNPK+VAFVGSGPLPLTSII+AMQHMK TYFDNYDIDSMAN+VAG
Sbjct: 114 KLAHLEYKILIDNGIVNPKRVAFVGSGPLPLTSIILAMQHMKNTYFDNYDIDSMANEVAG 173

Query: 123 KIVASDAELEGRMKFWTRDIVEVKEELGGYDCVFLAALVGMKKEEKVKILGHLRKYMKKV 182
           KIVASD ELEGRMKFWTRDIVEV EEL GYDCVFLAALVG+KKE+KVKIL HLRKYMK+ 
Sbjct: 174 KIVASDGELEGRMKFWTRDIVEVGEELEGYDCVFLAALVGLKKEDKVKILSHLRKYMKQG 233

Query: 183 G 184
           G
Sbjct: 234 G 234

[Close]

BLAST of Spg010650 vs. NCBI nr
Match: KAG7023395.1 (Nicotianamine synthase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 215.7 bits (548), Expect = 3.4e-52
Identity = 105/121 (86.78%), Postives = 113/121 (93.39%), Query Frame = 0


Query: 63  QLAHLEHKILNDNGVVNPKKVAFVGSGPLPLTSIIMAMQHMKTTYFDNYDIDSMANDVAG 122
           +LAHLE+KIL DNGVVNPKKVAFVGSGPLPLTSII+AMQHMK TYFDNYD+DSMAN+VAG
Sbjct: 114 KLAHLEYKILIDNGVVNPKKVAFVGSGPLPLTSIILAMQHMKNTYFDNYDVDSMANEVAG 173

Query: 123 KIVASDAELEGRMKFWTRDIVEVKEELGGYDCVFLAALVGMKKEEKVKILGHLRKYMKKV 182
           KIVASD ELEGRMKFWTRDIVEV EEL GYDCVFLAALVG+ KE+KVKIL HLRKYMK+ 
Sbjct: 174 KIVASDGELEGRMKFWTRDIVEVGEELEGYDCVFLAALVGLNKEDKVKILSHLRKYMKQG 233

Query: 183 G 184
           G
Sbjct: 234 G 234

[Close]

BLAST of Spg010650 vs. NCBI nr
Match: KAG6589715.1 (Nicotianamine synthase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 215.7 bits (548), Expect = 3.4e-52
Identity = 105/121 (86.78%), Postives = 113/121 (93.39%), Query Frame = 0


Query: 63  QLAHLEHKILNDNGVVNPKKVAFVGSGPLPLTSIIMAMQHMKTTYFDNYDIDSMANDVAG 122
           +LAHLE+KIL DNGVVNPKKVAFVGSGPLPLTSII+AMQHMK TYFDNYD+DSMAN+VAG
Sbjct: 114 KLAHLEYKILIDNGVVNPKKVAFVGSGPLPLTSIILAMQHMKNTYFDNYDVDSMANEVAG 173

Query: 123 KIVASDAELEGRMKFWTRDIVEVKEELGGYDCVFLAALVGMKKEEKVKILGHLRKYMKKV 182
           KIVASD ELEGRMKFWTRDIVEV EEL GYDCVFLAALVG+ KE+KVKIL HLRKYMK+ 
Sbjct: 174 KIVASDGELEGRMKFWTRDIVEVGEELEGYDCVFLAALVGLNKEDKVKILSHLRKYMKQG 233

Query: 183 G 184
           G
Sbjct: 234 G 234

[Close]

BLAST of Spg010650 vs. ExPASy Swiss-Prot
Match: Q9XGI7 (Nicotianamine synthase OS=Solanum lycopersicum OX=4081 GN=CHLN PE=2 SV=1)

HSP 1 Score: 132.9 bits (333), Expect = 3.8e-30
Identity = 67/130 (51.54%), Postives = 88/130 (67.69%), Query Frame = 0


Query: 50  QQSHSISILPELHQLAHLEHKILNDNGVVNPKKVAFVGSGPLPLTSIIMAMQHMKTTYFD 109
           Q  H         +L+ LE+ IL  N    PKK+AF+GSGPLPLTS+++A +H+KTT F 
Sbjct: 92  QHLHIFPYFDNYIKLSLLEYNILTKNTTNIPKKIAFIGSGPLPLTSLVLATKHLKTTCFH 151

Query: 110 NYDIDSMANDVAGKIVASDAELEGRMKFWTRDIVEVKEELGGYDCVFLAALVGMKKEEKV 169
           NYDID  AN +A  +VA+D ++  RM F T D+++V   L  YD VFLAALVGM KE+KV
Sbjct: 152 NYDIDVDANFMASALVAADPDMSSRMTFHTADVMDVTCALKDYDVVFLAALVGMDKEDKV 211

Query: 170 KILGHLRKYM 180
           K++ HL KYM
Sbjct: 212 KVVDHLAKYM 221

[Close]

BLAST of Spg010650 vs. ExPASy Swiss-Prot
Match: O80483 (Nicotianamine synthase 3 OS=Arabidopsis thaliana OX=3702 GN=NAS3 PE=2 SV=1)

HSP 1 Score: 127.5 bits (319), Expect = 1.6e-28
Identity = 70/132 (53.03%), Postives = 92/132 (69.70%), Query Frame = 0


Query: 53  HSISILPELH---QLAHLEHKIL--NDNGVVNPKKVAFVGSGPLPLTSIIMAMQHMKTTY 112
           H ++I P  +   +L  LE  +L  N NG V PK VAF+GSGPLPLTSI++A  H+K T 
Sbjct: 93  HHLNIFPYYNNYLKLGKLEFDLLEQNLNGFV-PKSVAFIGSGPLPLTSIVLASFHLKDTI 152

Query: 113 FDNYDIDSMANDVAGKIVASDAELEGRMKFWTRDIVEVKEELGGYDCVFLAALVGMKKEE 172
           F N+DID  AN +A  +V+SD ++  RM F T DI++V E L  +D VFLAALVGM KEE
Sbjct: 153 FHNFDIDPSANSLASLLVSSDPDISQRMFFHTVDIMDVTESLKSFDVVFLAALVGMNKEE 212

Query: 173 KVKILGHLRKYM 180
           KVK++ HL+K+M
Sbjct: 213 KVKVIEHLQKHM 223

[Close]

BLAST of Spg010650 vs. ExPASy Swiss-Prot
Match: Q9C7X5 (Probable nicotianamine synthase 4 OS=Arabidopsis thaliana OX=3702 GN=NAS4 PE=2 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 2.7e-28
Identity = 68/132 (51.52%), Postives = 94/132 (71.21%), Query Frame = 0


Query: 53  HSISILPELH---QLAHLEHKIL--NDNGVVNPKKVAFVGSGPLPLTSIIMAMQHMKTTY 112
           H +++ P  +   +L+ LE  +L  N NG V P+ VAF+GSGPLPLTS+++A  H+K + 
Sbjct: 94  HHLNLFPYYNNYLKLSKLEFDLLEQNLNGFV-PRTVAFIGSGPLPLTSVVLASSHLKDSI 153

Query: 113 FDNYDIDSMANDVAGKIVASDAELEGRMKFWTRDIVEVKEELGGYDCVFLAALVGMKKEE 172
           F N+DID  AN VA ++V+SD +L  RM F T DI++V E L G+D VFLAALVGM K+E
Sbjct: 154 FHNFDIDPSANMVAARLVSSDPDLSQRMFFHTVDIMDVTESLKGFDVVFLAALVGMDKKE 213

Query: 173 KVKILGHLRKYM 180
           KVK++ HL K+M
Sbjct: 214 KVKVVEHLEKHM 224

[Close]

BLAST of Spg010650 vs. ExPASy Swiss-Prot
Match: Q9FKT9 (Nicotianamine synthase 2 OS=Arabidopsis thaliana OX=3702 GN=NAS2 PE=2 SV=1)

HSP 1 Score: 121.3 bits (303), Expect = 1.1e-26
Identity = 61/117 (52.14%), Postives = 80/117 (68.38%), Query Frame = 0


Query: 63  QLAHLEHKILNDNGVVNPKKVAFVGSGPLPLTSIIMAMQHMKTTYFDNYDIDSMANDVAG 122
           +L  LE  +L+ +    P KVAF+GSGP+PLTSI++A  H+  T F N+DIDS AN +A 
Sbjct: 107 KLGKLEFDLLSQHTTHVPTKVAFIGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLAS 166

Query: 123 KIVASDAELEGRMKFWTRDIVEVKEELGGYDCVFLAALVGMKKEEKVKILGHLRKYM 180
            +V+ D++L  RM F T D++  KE L  YD VFLAALVGM KE KVK + HL K+M
Sbjct: 167 NLVSRDSDLSKRMIFHTTDVLNAKEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHM 223

[Close]

BLAST of Spg010650 vs. ExPASy Swiss-Prot
Match: Q9FF79 (Nicotianamine synthase 1 OS=Arabidopsis thaliana OX=3702 GN=NAS1 PE=2 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 9.6e-26
Identity = 60/117 (51.28%), Postives = 78/117 (66.67%), Query Frame = 0


Query: 63  QLAHLEHKILNDNGVVNPKKVAFVGSGPLPLTSIIMAMQHMKTTYFDNYDIDSMANDVAG 122
           +L  LE  +L+ +    P K+AFVGSGP+PLTSI++A  H+  T F N+DIDS AN +A 
Sbjct: 108 KLGKLEFDLLSQHSSHVPTKIAFVGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLAS 167

Query: 123 KIVASDAELEGRMKFWTRDIVEVKEELGGYDCVFLAALVGMKKEEKVKILGHLRKYM 180
            +V+ D +L  RM F T D++   E L  YD VFLAALVGM KE KVK + HL K+M
Sbjct: 168 NLVSRDPDLSKRMIFHTTDVLNATEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHM 224

[Close]

BLAST of Spg010650 vs. ExPASy TrEMBL
Match: A0A6J1JBQ8 (nicotianamine synthase-like OS=Cucurbita maxima OX=3661 GN=LOC111485338 PE=3 SV=1)

HSP 1 Score: 218.0 bits (554), Expect = 3.3e-53
Identity = 107/121 (88.43%), Postives = 114/121 (94.21%), Query Frame = 0


Query: 63  QLAHLEHKILNDNGVVNPKKVAFVGSGPLPLTSIIMAMQHMKTTYFDNYDIDSMANDVAG 122
           +LAHLE+KIL DNGVVNPKKVAFVGSGPLPLTSII+AMQHMK TYFDNYDIDSMAN+VAG
Sbjct: 114 KLAHLEYKILIDNGVVNPKKVAFVGSGPLPLTSIILAMQHMKNTYFDNYDIDSMANEVAG 173

Query: 123 KIVASDAELEGRMKFWTRDIVEVKEELGGYDCVFLAALVGMKKEEKVKILGHLRKYMKKV 182
           KIVASD ELEGRMKFWTRDIVEV EEL GYDCVFLAALVG+KKE+KVKIL HLRKYMK+ 
Sbjct: 174 KIVASDGELEGRMKFWTRDIVEVGEELEGYDCVFLAALVGLKKEDKVKILSHLRKYMKQG 233

Query: 183 G 184
           G
Sbjct: 234 G 234

[Close]

BLAST of Spg010650 vs. ExPASy TrEMBL
Match: A0A6J1E292 (nicotianamine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111430119 PE=3 SV=1)

HSP 1 Score: 215.7 bits (548), Expect = 1.6e-52
Identity = 105/121 (86.78%), Postives = 113/121 (93.39%), Query Frame = 0


Query: 63  QLAHLEHKILNDNGVVNPKKVAFVGSGPLPLTSIIMAMQHMKTTYFDNYDIDSMANDVAG 122
           +LAHLE+KIL DNGVVNPKKVAFVGSGPLPLTSII+AMQHMK TYFDNYD+DSMAN+VAG
Sbjct: 114 KLAHLEYKILIDNGVVNPKKVAFVGSGPLPLTSIILAMQHMKNTYFDNYDVDSMANEVAG 173

Query: 123 KIVASDAELEGRMKFWTRDIVEVKEELGGYDCVFLAALVGMKKEEKVKILGHLRKYMKKV 182
           KIVASD ELEGRMKFWTRDIVEV EEL GYDCVFLAALVG+ KE+KVKIL HLRKYMK+ 
Sbjct: 174 KIVASDGELEGRMKFWTRDIVEVGEELEGYDCVFLAALVGLNKEDKVKILSHLRKYMKQG 233

Query: 183 G 184
           G
Sbjct: 234 G 234

[Close]

BLAST of Spg010650 vs. ExPASy TrEMBL
Match: A0A5D3CIT2 (Nicotianamine synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold152G00680 PE=3 SV=1)

HSP 1 Score: 210.7 bits (535), Expect = 5.3e-51
Identity = 105/124 (84.68%), Postives = 114/124 (91.94%), Query Frame = 0


Query: 63  QLAHLEHKILNDNGVVNPKKVAFVGSGPLPLTSIIMAMQHMKTTYFDNYDIDSMANDVAG 122
           +LA+LE+KILNDNG+VNPKKVAFVGSGPLPLTSIIMAMQHMK TYFDNYD+DS+ANDVA 
Sbjct: 114 KLANLENKILNDNGIVNPKKVAFVGSGPLPLTSIIMAMQHMKGTYFDNYDVDSVANDVAR 173

Query: 123 KIVASDAELEGRMKFWTRDIVEVKEELGGYDCVFLAALVGMKKEEKVKILGHLRKYMKKV 182
           KIV SDAELEGRMKF + DIVEVKEELGGYDCVFLAALVGM KEEKVKI+ HLRKYMK+ 
Sbjct: 174 KIVGSDAELEGRMKFCSSDIVEVKEELGGYDCVFLAALVGMNKEEKVKIVRHLRKYMKEG 233

Query: 183 GFWL 187
           G  L
Sbjct: 234 GILL 237

[Close]

BLAST of Spg010650 vs. ExPASy TrEMBL
Match: A0A5A7UXS8 (Nicotianamine synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G006240 PE=3 SV=1)

HSP 1 Score: 210.7 bits (535), Expect = 5.3e-51
Identity = 105/124 (84.68%), Postives = 114/124 (91.94%), Query Frame = 0


Query: 63  QLAHLEHKILNDNGVVNPKKVAFVGSGPLPLTSIIMAMQHMKTTYFDNYDIDSMANDVAG 122
           +LA+LE+KILNDNG+VNPKKVAFVGSGPLPLTSIIMAMQHMK TYFDNYD+DS+ANDVA 
Sbjct: 114 KLANLENKILNDNGIVNPKKVAFVGSGPLPLTSIIMAMQHMKGTYFDNYDVDSVANDVAR 173

Query: 123 KIVASDAELEGRMKFWTRDIVEVKEELGGYDCVFLAALVGMKKEEKVKILGHLRKYMKKV 182
           KIV SDAELEGRMKF + DIVEVKEELGGYDCVFLAALVGM KEEKVKI+ HLRKYMK+ 
Sbjct: 174 KIVGSDAELEGRMKFCSSDIVEVKEELGGYDCVFLAALVGMNKEEKVKIVRHLRKYMKEG 233

Query: 183 GFWL 187
           G  L
Sbjct: 234 GILL 237

[Close]

BLAST of Spg010650 vs. ExPASy TrEMBL
Match: A0A1S3BXH0 (nicotianamine synthase-like OS=Cucumis melo OX=3656 GN=LOC103494275 PE=3 SV=1)

HSP 1 Score: 210.7 bits (535), Expect = 5.3e-51
Identity = 105/124 (84.68%), Postives = 114/124 (91.94%), Query Frame = 0


Query: 63  QLAHLEHKILNDNGVVNPKKVAFVGSGPLPLTSIIMAMQHMKTTYFDNYDIDSMANDVAG 122
           +LA+LE+KILNDNG+VNPKKVAFVGSGPLPLTSIIMAMQHMK TYFDNYD+DS+ANDVA 
Sbjct: 114 KLANLENKILNDNGIVNPKKVAFVGSGPLPLTSIIMAMQHMKGTYFDNYDVDSVANDVAR 173

Query: 123 KIVASDAELEGRMKFWTRDIVEVKEELGGYDCVFLAALVGMKKEEKVKILGHLRKYMKKV 182
           KIV SDAELEGRMKF + DIVEVKEELGGYDCVFLAALVGM KEEKVKI+ HLRKYMK+ 
Sbjct: 174 KIVGSDAELEGRMKFCSSDIVEVKEELGGYDCVFLAALVGMNKEEKVKIVRHLRKYMKEG 233

Query: 183 GFWL 187
           G  L
Sbjct: 234 GILL 237

[Close]

BLAST of Spg010650 vs. TAIR 10
Match: AT1G09240.1 (nicotianamine synthase 3 )

HSP 1 Score: 127.5 bits (319), Expect = 1.1e-29
Identity = 70/132 (53.03%), Postives = 92/132 (69.70%), Query Frame = 0


Query: 53  HSISILPELH---QLAHLEHKIL--NDNGVVNPKKVAFVGSGPLPLTSIIMAMQHMKTTY 112
           H ++I P  +   +L  LE  +L  N NG V PK VAF+GSGPLPLTSI++A  H+K T 
Sbjct: 93  HHLNIFPYYNNYLKLGKLEFDLLEQNLNGFV-PKSVAFIGSGPLPLTSIVLASFHLKDTI 152

Query: 113 FDNYDIDSMANDVAGKIVASDAELEGRMKFWTRDIVEVKEELGGYDCVFLAALVGMKKEE 172
           F N+DID  AN +A  +V+SD ++  RM F T DI++V E L  +D VFLAALVGM KEE
Sbjct: 153 FHNFDIDPSANSLASLLVSSDPDISQRMFFHTVDIMDVTESLKSFDVVFLAALVGMNKEE 212

Query: 173 KVKILGHLRKYM 180
           KVK++ HL+K+M
Sbjct: 213 KVKVIEHLQKHM 223

[Close]

BLAST of Spg010650 vs. TAIR 10
Match: AT1G56430.1 (nicotianamine synthase 4 )

HSP 1 Score: 126.7 bits (317), Expect = 1.9e-29
Identity = 68/132 (51.52%), Postives = 94/132 (71.21%), Query Frame = 0


Query: 53  HSISILPELH---QLAHLEHKIL--NDNGVVNPKKVAFVGSGPLPLTSIIMAMQHMKTTY 112
           H +++ P  +   +L+ LE  +L  N NG V P+ VAF+GSGPLPLTS+++A  H+K + 
Sbjct: 94  HHLNLFPYYNNYLKLSKLEFDLLEQNLNGFV-PRTVAFIGSGPLPLTSVVLASSHLKDSI 153

Query: 113 FDNYDIDSMANDVAGKIVASDAELEGRMKFWTRDIVEVKEELGGYDCVFLAALVGMKKEE 172
           F N+DID  AN VA ++V+SD +L  RM F T DI++V E L G+D VFLAALVGM K+E
Sbjct: 154 FHNFDIDPSANMVAARLVSSDPDLSQRMFFHTVDIMDVTESLKGFDVVFLAALVGMDKKE 213

Query: 173 KVKILGHLRKYM 180
           KVK++ HL K+M
Sbjct: 214 KVKVVEHLEKHM 224

[Close]

BLAST of Spg010650 vs. TAIR 10
Match: AT5G56080.1 (nicotianamine synthase 2 )

HSP 1 Score: 121.3 bits (303), Expect = 8.1e-28
Identity = 61/117 (52.14%), Postives = 80/117 (68.38%), Query Frame = 0


Query: 63  QLAHLEHKILNDNGVVNPKKVAFVGSGPLPLTSIIMAMQHMKTTYFDNYDIDSMANDVAG 122
           +L  LE  +L+ +    P KVAF+GSGP+PLTSI++A  H+  T F N+DIDS AN +A 
Sbjct: 107 KLGKLEFDLLSQHTTHVPTKVAFIGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLAS 166

Query: 123 KIVASDAELEGRMKFWTRDIVEVKEELGGYDCVFLAALVGMKKEEKVKILGHLRKYM 180
            +V+ D++L  RM F T D++  KE L  YD VFLAALVGM KE KVK + HL K+M
Sbjct: 167 NLVSRDSDLSKRMIFHTTDVLNAKEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHM 223

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BLAST of Spg010650 vs. TAIR 10
Match: AT5G04950.1 (nicotianamine synthase 1 )

HSP 1 Score: 118.2 bits (295), Expect = 6.9e-27
Identity = 60/117 (51.28%), Postives = 78/117 (66.67%), Query Frame = 0


Query: 63  QLAHLEHKILNDNGVVNPKKVAFVGSGPLPLTSIIMAMQHMKTTYFDNYDIDSMANDVAG 122
           +L  LE  +L+ +    P K+AFVGSGP+PLTSI++A  H+  T F N+DIDS AN +A 
Sbjct: 108 KLGKLEFDLLSQHSSHVPTKIAFVGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLAS 167

Query: 123 KIVASDAELEGRMKFWTRDIVEVKEELGGYDCVFLAALVGMKKEEKVKILGHLRKYM 180
            +V+ D +L  RM F T D++   E L  YD VFLAALVGM KE KVK + HL K+M
Sbjct: 168 NLVSRDPDLSKRMIFHTTDVLNATEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHM 224

The following BLAST results are available for this feature:

BLAST of Spg010650 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038880344.1	1.4e-53	88.43	nicotianamine synthase-like [Benincasa hispida]	[more]
XP_022987937.1	6.8e-53	88.43	nicotianamine synthase-like [Cucurbita maxima]	[more]
XP_023516742.1	2.0e-52	86.78	nicotianamine synthase-like [Cucurbita pepo subsp. pepo] >XP_023521836.1 nicotia...	[more]
KAG7023395.1	3.4e-52	86.78	Nicotianamine synthase, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6589715.1	3.4e-52	86.78	Nicotianamine synthase, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of Spg010650 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9XGI7	3.8e-30	51.54	Nicotianamine synthase OS=Solanum lycopersicum OX=4081 GN=CHLN PE=2 SV=1	[more]
O80483	1.6e-28	53.03	Nicotianamine synthase 3 OS=Arabidopsis thaliana OX=3702 GN=NAS3 PE=2 SV=1	[more]
Q9C7X5	2.7e-28	51.52	Probable nicotianamine synthase 4 OS=Arabidopsis thaliana OX=3702 GN=NAS4 PE=2 S...	[more]
Q9FKT9	1.1e-26	52.14	Nicotianamine synthase 2 OS=Arabidopsis thaliana OX=3702 GN=NAS2 PE=2 SV=1	[more]
Q9FF79	9.6e-26	51.28	Nicotianamine synthase 1 OS=Arabidopsis thaliana OX=3702 GN=NAS1 PE=2 SV=1	[more]

BLAST of Spg010650 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JBQ8	3.3e-53	88.43	nicotianamine synthase-like OS=Cucurbita maxima OX=3661 GN=LOC111485338 PE=3 SV=...	[more]
A0A6J1E292	1.6e-52	86.78	nicotianamine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111430119 PE=3 S...	[more]
A0A5D3CIT2	5.3e-51	84.68	Nicotianamine synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf...	[more]
A0A5A7UXS8	5.3e-51	84.68	Nicotianamine synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaf...	[more]
A0A1S3BXH0	5.3e-51	84.68	nicotianamine synthase-like OS=Cucumis melo OX=3656 GN=LOC103494275 PE=3 SV=1	[more]

BLAST of Spg010650 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G09240.1	1.1e-29	53.03	nicotianamine synthase 3	[more]
AT1G56430.1	1.9e-29	51.52	nicotianamine synthase 4	[more]
AT5G56080.1	8.1e-28	52.14	nicotianamine synthase 2	[more]
AT5G04950.1	6.9e-27	51.28	nicotianamine synthase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004298	Nicotianamine synthase	PFAM	PF03059	NAS	coord: 63..181 e-value: 7.2E-44 score: 150.2
IPR004298	Nicotianamine synthase	PANTHER	PTHR32266	NICOTIANAMINE SYNTHASE 3	coord: 63..184
IPR004298	Nicotianamine synthase	PROSITE	PS51142	NAS	coord: 1..186 score: 18.798449
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 39..184 e-value: 2.6E-26 score: 94.3
None	No IPR available	PANTHER	PTHR32266:SF11	NICOTIANAMINE SYNTHASE	coord: 63..184

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg010650.1	Spg010650.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030418	nicotianamine biosynthetic process
molecular_function	GO:0030410	nicotianamine synthase activity