Spg008663 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg008663 vs. NCBI nr
Match: XP_022990956.1 (MACPF domain-containing protein At1g14780 [Cucurbita maxima])

HSP 1 Score: 1109.7 bits (2869), Expect = 0.0e+00
Identity = 545/640 (85.16%), Postives = 579/640 (90.47%), Query Frame = 0

Query: 1   MGDGIVETALSSLGKGFDLTSDFRLKYCKGERRLVLLNEAQRRDLIVPGFGAVQGVSIDI 60
           MGDGIV+ AL+SLGKGFDLTSDF+LKYCKG+ RLVLLN+AQRRDLIVPGFGAV+ +SIDI
Sbjct: 1   MGDGIVDAALASLGKGFDLTSDFKLKYCKGQHRLVLLNQAQRRDLIVPGFGAVKDISIDI 60

Query: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNAMFGFQSGCWAMDAVNTKCL 120
           KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNAMFGFQSGCWAMDA NTKCL
Sbjct: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNAMFGFQSGCWAMDAENTKCL 120

Query: 121 GLDGYFISLFNAHIDRYPLRLSDQVRSAVPSSWDPPAIARFIEKYGTHIIVGLSIGGHDV 180
           GLDGYFISLFNAHIDRYPLRLSD+VR+AVP+SWDPPAIARFIEKYGTHIIVGLSIGGHDV
Sbjct: 121 GLDGYFISLFNAHIDRYPLRLSDEVRAAVPTSWDPPAIARFIEKYGTHIIVGLSIGGHDV 180

Query: 181 VLVRQDKSSTLEPSELKNHLEELGDQLFTGACTFPPPLSKPKDHHKTKAPQAFNLFDPQL 240
           VLVRQDKSS LEPSELK+HLEELGDQLFTGACTFPPP SKPKDHHKTK PQAFNLFDP+L
Sbjct: 181 VLVRQDKSSNLEPSELKSHLEELGDQLFTGACTFPPPHSKPKDHHKTKLPQAFNLFDPKL 240

Query: 241 VALGNFSSVSAKDGIIVITSKRGGSATASSHCEWLPTVLESPDAIHFQFIPIISLLEGVP 300
           VA  NFSSVSAKDGIIVITSK+GGS TAS+HCEWLPTVLESPDAIHFQFIPIISLL+ +P
Sbjct: 241 VAYANFSSVSAKDGIIVITSKKGGSPTASTHCEWLPTVLESPDAIHFQFIPIISLLDRIP 300

Query: 301 GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNITTSPSLQLNLM 360
           GKGFLSHAINLYLRYKPPIADLQYFLDFQ+HKIWAPIHNDLPLGPTPNITTSP LQ NLM
Sbjct: 301 GKGFLSHAINLYLRYKPPIADLQYFLDFQMHKIWAPIHNDLPLGPTPNITTSPYLQPNLM 360

Query: 361 GPKLYVNTTQVTAENKPVTGMRLYLEGIKSNRLAIHIQHLLTTPLLFENNITQQTPPLWY 420
           GPKLYVNTTQVT+ENKPVTG+RLYLEGIKSNRLAIHIQHL TTP+L +NNITQ  PPLW+
Sbjct: 361 GPKLYVNTTQVTSENKPVTGLRLYLEGIKSNRLAIHIQHLQTTPILLQNNITQHNPPLWH 420

Query: 421 SSQHIADDRYIEPLNRNKFSYVCTAPVKYDPIWATAVAASSSSSNFSPSSSSSPSSPSSP 480
           SS H+ADDRY+EPLNRNKFSYV TAPVKYDP WATA A                    +P
Sbjct: 421 SSLHVADDRYVEPLNRNKFSYVSTAPVKYDPTWATATA--------------------TP 480

Query: 481 TADADSPAAFIVTGAQLHVIKQQDSKTVLHLRLLYSKVSNSCVVQTSWARHSSDISPKSG 540
           T     PAAFIVTGAQLHV+K  DSK  LHL LLYSKVSNS VVQT+WARHSSDISPKSG
Sbjct: 481 T-----PAAFIVTGAQLHVVKHHDSKAALHLGLLYSKVSNSYVVQTNWARHSSDISPKSG 540

Query: 541 IFSAISTSLSGAAAVPKEKPDAAAAVIVDSGVFHGGPPVPLQAQKLVKFVELSEMRKGPQ 600
           IFSAISTSLSG+A+ PK+KP   A VIVDSG+FH GPPVPLQAQKLVKFVELSEMRKGPQ
Sbjct: 541 IFSAISTSLSGSASAPKKKPPDPATVIVDSGIFHHGPPVPLQAQKLVKFVELSEMRKGPQ 600

Query: 601 DSPGHWVVIGARLSLEKGKISLHVKFGLLHVVSETN-DEQ 640
           DSPGHWVVIGARL+LE GKI LHVKFGL+HVVSET+ DEQ
Sbjct: 601 DSPGHWVVIGARLNLENGKICLHVKFGLVHVVSETSGDEQ 615

BLAST of Spg008663 vs. NCBI nr
Match: XP_038874758.1 (MACPF domain-containing protein At1g14780 [Benincasa hispida])

HSP 1 Score: 1108.2 bits (2865), Expect = 0.0e+00
Identity = 554/640 (86.56%), Postives = 577/640 (90.16%), Query Frame = 0

Query: 1   MGDGIVETALSSLGKGFDLTSDFRLKYCKGERRLVLLNEAQRRDLIVPGFGAVQGVSIDI 60
           MGD IV+ AL+SLGKGFDLTSDFRLKYCKGE  LVLLN++QRRDLIVPGFGAV  VSIDI
Sbjct: 1   MGDEIVKIALNSLGKGFDLTSDFRLKYCKGEHSLVLLNQSQRRDLIVPGFGAVNDVSIDI 60

Query: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNAMFGFQSGCWAMDAVNTKCL 120
           KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFN+MFGFQSGCWAMDA+NTKCL
Sbjct: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNSMFGFQSGCWAMDAMNTKCL 120

Query: 121 GLDGYFISLFNAHIDRYPLRLSDQVRSAVPSSWDPPAIARFIEKYGTHIIVGLSIGGHDV 180
           GLDGYFISLFNAHIDRYPLRLSD+VRSAVPSSWDP AIARF+EKYGTHIIVGLS+GGHDV
Sbjct: 121 GLDGYFISLFNAHIDRYPLRLSDEVRSAVPSSWDPLAIARFVEKYGTHIIVGLSVGGHDV 180

Query: 181 VLVRQDKSSTLEPSELKNHLEELGDQLFTGACTFPPPLSKPKDHHKTKAPQAFNLFDPQL 240
           VLVRQDKSS LEPSELKNHLEELGDQLF GACTFPPPLSKPKDHHK K PQAFNLFDPQL
Sbjct: 181 VLVRQDKSSNLEPSELKNHLEELGDQLFNGACTFPPPLSKPKDHHKPKTPQAFNLFDPQL 240

Query: 241 VALGNFSSVSAKDGIIVITSKRGGSATASSHCEWLPTVLESPDAIHFQFIPIISLLEGVP 300
           VA GNFSSVSAKDGIIVITSKRGGS TASSHCEWLPTV ESPDAIHFQFIPIISLLEG+P
Sbjct: 241 VAFGNFSSVSAKDGIIVITSKRGGSPTASSHCEWLPTVHESPDAIHFQFIPIISLLEGMP 300

Query: 301 GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNITTSPSLQLNLM 360
           GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNITT PSLQLNLM
Sbjct: 301 GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNITTFPSLQLNLM 360

Query: 361 GPKLYVNTTQVTAENKPVTGMRLYLEGIKSNRLAIHIQHLLTTPLLFENNITQQTPPLWY 420
           GPKLYVNTTQV  ENKPVTGMRLYLEGIKSNRLAIHIQHLLTTPLL ENNITQQT   W+
Sbjct: 361 GPKLYVNTTQVIVENKPVTGMRLYLEGIKSNRLAIHIQHLLTTPLLLENNITQQT--YWH 420

Query: 421 SSQHIADDRYIEPLNRNKFSYVCTAPVKYDPIWATAVAASSSSSNFSPSSSSSPSSPSSP 480
           SSQHIAD+RY+E LNRNKFS+VCTAP+KYDP WAT                      +S 
Sbjct: 421 SSQHIADERYVESLNRNKFSHVCTAPIKYDPAWAT----------------------TSG 480

Query: 481 TADADSPAAFIVTGAQLHVIKQQDSKTVLHLRLLYSKVSNSCVVQTSWARHSSDISPKSG 540
             D  SP AFIVTGAQLHV+KQ DSKTVLHLRLLYSKVSNS VVQTSWARHSSDISPKSG
Sbjct: 481 NHDFPSPTAFIVTGAQLHVVKQHDSKTVLHLRLLYSKVSNSYVVQTSWARHSSDISPKSG 540

Query: 541 IFSAISTSLSGAAAVPKEKPDAAAAVIVDSGVFHGGPPVPLQAQKLVKFVELSEMRKGPQ 600
           IFSAISTSL+G AA  KEKP   AAVIVDSGVFHGGPP+ +QAQKLVKFVELSEMRKGPQ
Sbjct: 541 IFSAISTSLTGVAAALKEKP-PDAAVIVDSGVFHGGPPMSVQAQKLVKFVELSEMRKGPQ 600

Query: 601 DSPGHWVVIGARLSLEKGKISLHVKFGLLHVVSET-NDEQ 640
           DSPGHW+VIGARL+LEKGKI LHVKFGLLHVVSET  D+Q
Sbjct: 601 DSPGHWMVIGARLNLEKGKICLHVKFGLLHVVSETITDDQ 615

BLAST of Spg008663 vs. NCBI nr
Match: XP_022952953.1 (MACPF domain-containing protein At1g14780 [Cucurbita moschata] >XP_022952961.1 MACPF domain-containing protein At1g14780 [Cucurbita moschata])

HSP 1 Score: 1103.2 bits (2852), Expect = 0.0e+00
Identity = 546/640 (85.31%), Postives = 574/640 (89.69%), Query Frame = 0

Query: 1   MGDGIVETALSSLGKGFDLTSDFRLKYCKGERRLVLLNEAQRRDLIVPGFGAVQGVSIDI 60
           MGDGIV+ AL+SLGKGFDLTSDF+LKYCKG+ RLVLLNEAQRRDLIVPGFG V+ VSIDI
Sbjct: 1   MGDGIVDAALASLGKGFDLTSDFKLKYCKGKHRLVLLNEAQRRDLIVPGFGPVKDVSIDI 60

Query: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNAMFGFQSGCWAMDAVNTKCL 120
           KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSG FNAMFGFQSGCWAMDAVNTKCL
Sbjct: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGFFNAMFGFQSGCWAMDAVNTKCL 120

Query: 121 GLDGYFISLFNAHIDRYPLRLSDQVRSAVPSSWDPPAIARFIEKYGTHIIVGLSIGGHDV 180
           GLDGYFISLFNAHIDRYPLRLSD+VR+AVPSSWDPPAIARFIEKYGTHIIVGLSIGGHDV
Sbjct: 121 GLDGYFISLFNAHIDRYPLRLSDEVRAAVPSSWDPPAIARFIEKYGTHIIVGLSIGGHDV 180

Query: 181 VLVRQDKSSTLEPSELKNHLEELGDQLFTGACTFPPPLSKPKDHHKTKAPQAFNLFDPQL 240
           VLVRQDKSS LE SELK+HLEELGDQLFTGACTFPPP SKPKDHHKTK PQAFNLFDP+L
Sbjct: 181 VLVRQDKSSNLEASELKSHLEELGDQLFTGACTFPPPHSKPKDHHKTKLPQAFNLFDPKL 240

Query: 241 VALGNFSSVSAKDGIIVITSKRGGSATASSHCEWLPTVLESPDAIHFQFIPIISLLEGVP 300
           VA  NFSSVSAKDGIIVITSK+GGS TAS+HCEWLPTVLESPDAIHFQFIPIISLL+ VP
Sbjct: 241 VAYANFSSVSAKDGIIVITSKKGGSPTASTHCEWLPTVLESPDAIHFQFIPIISLLDRVP 300

Query: 301 GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNITTSPSLQLNLM 360
           GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNITTSP LQ NLM
Sbjct: 301 GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNITTSPYLQPNLM 360

Query: 361 GPKLYVNTTQVTAENKPVTGMRLYLEGIKSNRLAIHIQHLLTTPLLFENNITQQTPPLWY 420
           GPKLYVNTTQVT+ENKPVTG+RLYLEGIKSNRLAIHIQHL TTP+L +NNITQ  PPLW+
Sbjct: 361 GPKLYVNTTQVTSENKPVTGLRLYLEGIKSNRLAIHIQHLQTTPILLQNNITQHNPPLWH 420

Query: 421 SSQHIADDRYIEPLNRNKFSYVCTAPVKYDPIWATAVAASSSSSNFSPSSSSSPSSPSSP 480
           SS H+ADDRY+EPLNRNKFSYV TAPV YDP WATA                      +P
Sbjct: 421 SSLHVADDRYVEPLNRNKFSYVSTAPVTYDPTWATA----------------------TP 480

Query: 481 TADADSPAAFIVTGAQLHVIKQQDSKTVLHLRLLYSKVSNSCVVQTSWARHSSDISPKSG 540
           T     PAAFIVTGAQLHV+K  DSK  LHL LLYSKVSNS +VQT+WARHSSDISPKSG
Sbjct: 481 T-----PAAFIVTGAQLHVVKHHDSKAALHLGLLYSKVSNSYIVQTNWARHSSDISPKSG 540

Query: 541 IFSAISTSLSGAAAVPKEKPDAAAAVIVDSGVFHGGPPVPLQAQKLVKFVELSEMRKGPQ 600
           IFSAISTSLSGA++ PKEKP   A VIVDSGVFH GPPVPLQAQKLVKFVELSEMRKGPQ
Sbjct: 541 IFSAISTSLSGASSAPKEKPPDPATVIVDSGVFHHGPPVPLQAQKLVKFVELSEMRKGPQ 600

Query: 601 DSPGHWVVIGARLSLEKGKISLHVKFGLLHVVSETN-DEQ 640
           DSPGHWVVIGARL+LE  KI LHVKFGL+HVVSET+ DEQ
Sbjct: 601 DSPGHWVVIGARLNLENSKICLHVKFGLVHVVSETSADEQ 613

BLAST of Spg008663 vs. NCBI nr
Match: KAG7032583.1 (MACPF domain-containing protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1101.7 bits (2848), Expect = 0.0e+00
Identity = 545/640 (85.16%), Postives = 574/640 (89.69%), Query Frame = 0

Query: 1   MGDGIVETALSSLGKGFDLTSDFRLKYCKGERRLVLLNEAQRRDLIVPGFGAVQGVSIDI 60
           MGDGIV+ AL+SLGKGFDLTSDF+LKYCKG+ RLVLLNEAQRRDLIVPGFG V+ VSIDI
Sbjct: 1   MGDGIVDAALASLGKGFDLTSDFKLKYCKGKHRLVLLNEAQRRDLIVPGFGPVKDVSIDI 60

Query: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNAMFGFQSGCWAMDAVNTKCL 120
           KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSG FNA+FGFQSGCWAMDAVNTKCL
Sbjct: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGFFNAIFGFQSGCWAMDAVNTKCL 120

Query: 121 GLDGYFISLFNAHIDRYPLRLSDQVRSAVPSSWDPPAIARFIEKYGTHIIVGLSIGGHDV 180
           GLDGYFISLFNAHIDRYPLRLSD+VR+AVPSSWDPPAIARFIEKYGTHIIVGLSIGGHDV
Sbjct: 121 GLDGYFISLFNAHIDRYPLRLSDEVRAAVPSSWDPPAIARFIEKYGTHIIVGLSIGGHDV 180

Query: 181 VLVRQDKSSTLEPSELKNHLEELGDQLFTGACTFPPPLSKPKDHHKTKAPQAFNLFDPQL 240
           VLVRQDKSS LE SELK+HLEELGDQLFTGACTFPPP SKPKDHHKTK PQAFNLFDP+L
Sbjct: 181 VLVRQDKSSNLEASELKSHLEELGDQLFTGACTFPPPHSKPKDHHKTKLPQAFNLFDPKL 240

Query: 241 VALGNFSSVSAKDGIIVITSKRGGSATASSHCEWLPTVLESPDAIHFQFIPIISLLEGVP 300
           VA  NFSSVSAKDGIIVITSK+GGS TAS+HCEWLPTVLESPDAIHFQFIPIISLL+ VP
Sbjct: 241 VAYANFSSVSAKDGIIVITSKKGGSPTASTHCEWLPTVLESPDAIHFQFIPIISLLDRVP 300

Query: 301 GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNITTSPSLQLNLM 360
           GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNITTSP LQ NLM
Sbjct: 301 GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNITTSPYLQPNLM 360

Query: 361 GPKLYVNTTQVTAENKPVTGMRLYLEGIKSNRLAIHIQHLLTTPLLFENNITQQTPPLWY 420
           GPKLYVNTTQVT+ENKPVTG+RLYLEGIKSNRLAIHIQHL TTP+L +NNITQ  PPLW+
Sbjct: 361 GPKLYVNTTQVTSENKPVTGLRLYLEGIKSNRLAIHIQHLQTTPILLQNNITQHNPPLWH 420

Query: 421 SSQHIADDRYIEPLNRNKFSYVCTAPVKYDPIWATAVAASSSSSNFSPSSSSSPSSPSSP 480
           SS H+ADDRY+EPLNRNKFSYV TAPV YDP WATA                      +P
Sbjct: 421 SSLHVADDRYVEPLNRNKFSYVSTAPVTYDPTWATA----------------------TP 480

Query: 481 TADADSPAAFIVTGAQLHVIKQQDSKTVLHLRLLYSKVSNSCVVQTSWARHSSDISPKSG 540
           T     PAAFIVTGAQLHV+K  DSK  LHL LLYSKVSNS +VQT+WARHSSDISPKSG
Sbjct: 481 T-----PAAFIVTGAQLHVVKHHDSKAALHLGLLYSKVSNSYIVQTNWARHSSDISPKSG 540

Query: 541 IFSAISTSLSGAAAVPKEKPDAAAAVIVDSGVFHGGPPVPLQAQKLVKFVELSEMRKGPQ 600
           IFSAISTSLSGA++ PKEKP   A VIVDSGVFH GPPVPLQAQKLVKFVELSEMRKGPQ
Sbjct: 541 IFSAISTSLSGASSAPKEKPPDPATVIVDSGVFHHGPPVPLQAQKLVKFVELSEMRKGPQ 600

Query: 601 DSPGHWVVIGARLSLEKGKISLHVKFGLLHVVSETN-DEQ 640
           DSPGHWVVIGARL+LE  KI LHVKFGL+HVVSET+ DEQ
Sbjct: 601 DSPGHWVVIGARLNLENSKICLHVKFGLVHVVSETSADEQ 613

BLAST of Spg008663 vs. NCBI nr
Match: XP_023546039.1 (MACPF domain-containing protein At1g14780 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1100.9 bits (2846), Expect = 0.0e+00
Identity = 545/640 (85.16%), Postives = 573/640 (89.53%), Query Frame = 0

Query: 1   MGDGIVETALSSLGKGFDLTSDFRLKYCKGERRLVLLNEAQRRDLIVPGFGAVQGVSIDI 60
           MGDGIV+ AL+SLGKGFDLTSDF+LKYCKG+ RLVLLNEAQRRDLIVPGFGA++ VSIDI
Sbjct: 1   MGDGIVDAALASLGKGFDLTSDFKLKYCKGQHRLVLLNEAQRRDLIVPGFGAIKDVSIDI 60

Query: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNAMFGFQSGCWAMDAVNTKCL 120
           KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSG FNAMFGFQSGCWAMDAVNTKCL
Sbjct: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGFFNAMFGFQSGCWAMDAVNTKCL 120

Query: 121 GLDGYFISLFNAHIDRYPLRLSDQVRSAVPSSWDPPAIARFIEKYGTHIIVGLSIGGHDV 180
           GLDGYFISLFNAHIDRYPLRLSD+VR+AVPSSWDPPAIARFIEKYGTHIIVGLSIGG DV
Sbjct: 121 GLDGYFISLFNAHIDRYPLRLSDEVRAAVPSSWDPPAIARFIEKYGTHIIVGLSIGGQDV 180

Query: 181 VLVRQDKSSTLEPSELKNHLEELGDQLFTGACTFPPPLSKPKDHHKTKAPQAFNLFDPQL 240
           VLVRQDKSS L+PSELK+HLEELGDQLFTGACTFPPP SKPKDHHKTK PQAFNLFD +L
Sbjct: 181 VLVRQDKSSNLQPSELKSHLEELGDQLFTGACTFPPPHSKPKDHHKTKLPQAFNLFDTKL 240

Query: 241 VALGNFSSVSAKDGIIVITSKRGGSATASSHCEWLPTVLESPDAIHFQFIPIISLLEGVP 300
           VA  NFSSVSAKDGIIVITSK+GGS TAS+HCEWLPTVLESPDAIHFQFIPIISLL+ VP
Sbjct: 241 VAYANFSSVSAKDGIIVITSKKGGSPTASTHCEWLPTVLESPDAIHFQFIPIISLLDRVP 300

Query: 301 GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNITTSPSLQLNLM 360
           GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNITTSP LQ NLM
Sbjct: 301 GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNITTSPYLQPNLM 360

Query: 361 GPKLYVNTTQVTAENKPVTGMRLYLEGIKSNRLAIHIQHLLTTPLLFENNITQQTPPLWY 420
           GPKLYVNTTQVT+E KPVTG+RLYLEGIKSNRLAIHIQHL TTP+L +NNITQ  PPLW+
Sbjct: 361 GPKLYVNTTQVTSEKKPVTGLRLYLEGIKSNRLAIHIQHLQTTPILLQNNITQHNPPLWH 420

Query: 421 SSQHIADDRYIEPLNRNKFSYVCTAPVKYDPIWATAVAASSSSSNFSPSSSSSPSSPSSP 480
           SS H+ADDRY+EPLNRNKFSYV TAPVKYDP WATA                      +P
Sbjct: 421 SSLHVADDRYVEPLNRNKFSYVSTAPVKYDPTWATA----------------------TP 480

Query: 481 TADADSPAAFIVTGAQLHVIKQQDSKTVLHLRLLYSKVSNSCVVQTSWARHSSDISPKSG 540
           T     PAAFIVTGAQLHV+K  DSK  LHL LLYSKVSNS VVQT+WARHSSDISPKSG
Sbjct: 481 T-----PAAFIVTGAQLHVVKHHDSKAALHLGLLYSKVSNSYVVQTNWARHSSDISPKSG 540

Query: 541 IFSAISTSLSGAAAVPKEKPDAAAAVIVDSGVFHGGPPVPLQAQKLVKFVELSEMRKGPQ 600
           IFSAISTSLSGA + PKEKP   A VIVDSGVFH GPPVPLQAQKLVKFVELSEMRKGPQ
Sbjct: 541 IFSAISTSLSGATSAPKEKPPDPATVIVDSGVFHHGPPVPLQAQKLVKFVELSEMRKGPQ 600

Query: 601 DSPGHWVVIGARLSLEKGKISLHVKFGLLHVVSETN-DEQ 640
           DSPGHWVVIGARL+LE  KI LHVKFGL+HVVSET+ DEQ
Sbjct: 601 DSPGHWVVIGARLNLENSKICLHVKFGLVHVVSETSADEQ 613

BLAST of Spg008663 vs. ExPASy Swiss-Prot
Match: Q8L612 (MACPF domain-containing protein At1g14780 OS=Arabidopsis thaliana OX=3702 GN=At1g14780 PE=2 SV=1)

HSP 1 Score: 685.6 bits (1768), Expect = 5.2e-196
Identity = 360/646 (55.73%), Postives = 449/646 (69.50%), Query Frame = 0

Query: 2   GDGIVETALSSLGKGFDLTSDFRLKYCK------GERRLVLLNEAQRRDLIVPGFGAVQG 61
           G  ++ETA+ SLGKGFDLT+DFRLKYCK      G+ RLV+L++ Q R+L +PGFG  Q 
Sbjct: 5   GGDVIETAVKSLGKGFDLTADFRLKYCKDGDGSAGDDRLVVLDQTQNRELHIPGFGVFQN 64

Query: 62  VSIDIKCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNAMFGFQSGCWAMDAV 121
           VS DI CD+G+RTR++SDIL+FN+MSE FN++ S+ GKIPSG+FNA FGFQSG WA DA 
Sbjct: 65  VSADINCDKGERTRFRSDILDFNKMSEYFNQRSSVTGKIPSGNFNATFGFQSGSWATDAA 124

Query: 122 NTKCLGLDGYFISLFNAHI-DRYPLRLSDQVRSAVPSSWDPPAIARFIEKYGTHIIVGLS 181
           N K LGLD   ++LFN HI +   LRL+D+VR+AVPSSWDP  +ARFIE+YGTH+I G+S
Sbjct: 125 NVKSLGLDASVVTLFNLHIHNPNRLRLTDRVRNAVPSSWDPQLLARFIERYGTHVITGVS 184

Query: 182 IGGHDVVLVRQDKSSTLEPSELKNHLEELGDQLFTGACTFPPPLSKPKDHH---KTKAPQ 241
           +GG DVV+VRQDKSS L+   L++HL +LGDQLFTG+C           HH   + K P+
Sbjct: 185 VGGQDVVVVRQDKSSDLDNDLLRHHLYDLGDQLFTGSCLLSTRRLNKAYHHSHSQPKFPE 244

Query: 242 AFNLF-DPQLVALGNFSSVSAKDGIIVITSKRGGSATASSHCEWLPTVLESPDAIHFQFI 301
           AFN+F D Q VA  NF S+++++GI VI +KRGG   A SH EWL TV + PDAI+F FI
Sbjct: 245 AFNVFDDKQTVAFNNF-SINSQNGITVICAKRGGDGRAKSHSEWLITVPDKPDAINFNFI 304

Query: 302 PIISLLEGVPGKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNIT 361
           PI SLL+ VPG G LSHA++LYLRYKPP+ DLQYFLDF   + WAP+HNDLP G  PN+ 
Sbjct: 305 PITSLLKDVPGSGLLSHAMSLYLRYKPPLMDLQYFLDFSGPRAWAPVHNDLPFGAAPNMA 364

Query: 362 TS-PSLQLNLMGPKLYVNTTQVTAENKPVTGMRLYLEGIKSNRLAIHIQHLLTTPLLFEN 421
           ++ P+L +N MGPKLYVNTT VT+E  PVTGMR +LEG K NRLAIH+QHL  T      
Sbjct: 365 SAYPALHINFMGPKLYVNTTPVTSEKNPVTGMRFFLEGKKCNRLAIHLQHLDNTRTTVGE 424

Query: 422 NITQQTPPLWYSSQHIAD-DRYIEPLNRNKFSYVCTAPVKYDPIWATAVAASSSSSNFSP 481
            IT +   +W  S  I D DRY EPLN  KFS+VCT PVKYDP W    +   S ++   
Sbjct: 425 KITDE--HIWRGSDQITDNDRYFEPLNGKKFSHVCTVPVKYDPNWIKTTSNHKSQND--- 484

Query: 482 SSSSSPSSPSSPTADADSPAAFIVTGAQLHVIKQQDSKTVLHLRLLYSKVSNSCVVQTSW 541
                               AFIVTGAQL V K+  SK+VLHLRL Y+KVS+  VVQ SW
Sbjct: 485 -------------------VAFIVTGAQLEV-KKHGSKSVLHLRLRYTKVSDHYVVQNSW 544

Query: 542 ARHSSDISPKSGIFSAISTSLSGAAA----VPKEKPDAAAAVIVDSGVFHGGPPVPLQAQ 601
                  S KSGIFS++S  L+  +     + K+K +    V++DSGVF GGPPVP    
Sbjct: 545 VHGPIGTSQKSGIFSSMSMPLTSGSVHHNMIQKDKNE----VVLDSGVFPGGPPVPAN-N 604

Query: 602 KLVKFVELSEMRKGPQDSPGHWVVIGARLSLEKGKISLHVKFGLLH 631
           K+VKFV+LS++ +GPQ SPGHW+V G RL L+KGK+ LHVKF LLH
Sbjct: 605 KIVKFVDLSQLCRGPQHSPGHWLVTGVRLYLDKGKLCLHVKFALLH 619

BLAST of Spg008663 vs. ExPASy Swiss-Prot
Match: Q9STW5 (MACPF domain-containing protein At4g24290 OS=Arabidopsis thaliana OX=3702 GN=At4g24290 PE=2 SV=1)

HSP 1 Score: 499.2 bits (1284), Expect = 6.9e-140
Identity = 281/643 (43.70%), Postives = 385/643 (59.88%), Query Frame = 0

Query: 7   ETALSSLGKGFDLTSDFRLKYCKG---ERRLVLLNEAQRR-DLIVPGFGAVQGVSIDIKC 66
           E A+ S+G G+DL  D RLKYCKG   + RL+ + E     ++++PG  ++  VS  IKC
Sbjct: 12  EVAIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGISIPNVSKSIKC 71

Query: 67  DRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNAMFGFQSGCWAMDAVNTKCLGL 126
           D+G+R R++SDIL F QM+E FN++ S+ GKIPSG FNAMF F S CW  DA  TK L  
Sbjct: 72  DKGERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEF-SSCWQKDAAYTKNLAF 131

Query: 127 DGYFISLFNAHIDRYPLRLSDQVRSAVPSSWDPPAIARFIEKYGTHIIVGLSIGGHDVVL 186
           DG FISL++  +D+  + L + V+ AVPS+WDP A+ARFI+ YGTHIIV + +GG DV+ 
Sbjct: 132 DGVFISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVSVKMGGKDVIY 191

Query: 187 VRQDKSSTLEPSELKNHLEELGDQLFTGACTFPPPLSKPKDHHKTKAPQAFNLFDPQLVA 246
            +Q  SS L+P +L+  L+E+ D+ F  A       S+          +   L      +
Sbjct: 192 AKQQHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVETKEQRLRFADTSS 251

Query: 247 LGNFSSVSAKDGIIVITSKRGGSATAS-SHCEWLPTVLESPDAIHFQFIPIISLLEGVPG 306
           LG++++   K+  + +  +RGG+   +  H EWL TV   PD I   FIPI SLL GVPG
Sbjct: 252 LGSYAN---KEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFIPITSLLNGVPG 311

Query: 307 KGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNITTSPSLQLNLMG 366
            GFLSHAINLYLRYKPPI +L  FL+FQ+ + WAP+ ++LPLGP     +  SLQ +  G
Sbjct: 312 SGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPVFSELPLGPQRKQQSCASLQFSFFG 371

Query: 367 PKLYVNTTQVTAENKPVTGMRLYLEGIKSNRLAIHIQHLLTTPLLF--ENNITQQTPPLW 426
           PKLYVNTT V    +P+TGMRLYLEG +SNRLAIH+QHL + P ++  E+++ +      
Sbjct: 372 PKLYVNTTPVDVGKRPITGMRLYLEGRRSNRLAIHLQHLSSLPKIYQLEDDLNRSI---- 431

Query: 427 YSSQHIADDRYIEPLNRNKFSYVCTAPVKYDPIWATAVAASSSSSNFSPSSSSSPSSPSS 486
              Q   D RY E +N   +S+VCT PV+ D   +                         
Sbjct: 432 --RQESHDRRYYEKVNWKNYSHVCTEPVESDDDLS------------------------- 491

Query: 487 PTADADSPAAFIVTGAQLHVIKQQDSKTVLHLRLLYSKVSNSCVVQTSWARHSSDISPKS 546
                      +VTGAQLHV +    K VL LRL +S+V  + +V+ S    +   +PKS
Sbjct: 492 -----------VVTGAQLHV-ESHGFKNVLFLRLCFSRVVGATLVKNSEWDEAVGFAPKS 551

Query: 547 GIFSAISTSLSGAAAVPKEKPDAAAAVIVDSGVFHGGPPVPLQAQKLVKFVELSEMRKGP 606
           G+ S + +    AA  P  +P   A V ++S ++ GGPPVP QA KL+KFV+ SEM +GP
Sbjct: 552 GLISTLISHHFTAAQKPPPRP---ADVNINSAIYPGGPPVPTQAPKLLKFVDTSEMTRGP 604

Query: 607 QDSPGHWVVIGARLSLEKGKISLHVKFGLLHVV--SETNDEQY 641
           Q+SPG+WVV GARL +EKGKISL VK+ L   +   E  +E Y
Sbjct: 612 QESPGYWVVSGARLLVEKGKISLKVKYSLFTPILGDEVIEEAY 604

BLAST of Spg008663 vs. ExPASy Swiss-Prot
Match: Q9SGN6 (MACPF domain-containing protein NSL1 OS=Arabidopsis thaliana OX=3702 GN=NSL1 PE=2 SV=1)

HSP 1 Score: 459.9 bits (1182), Expect = 4.7e-128
Identity = 270/636 (42.45%), Postives = 370/636 (58.18%), Query Frame = 0

Query: 7   ETALSSLGKGFDLTSDFRLKYCK---GERRLVLLNEAQRRDLIVPGFGAVQGVSIDIKCD 66
           E A+S +G G+DL SD R   CK      RLV ++  + RDLI PG   V  VS  IKCD
Sbjct: 16  EKAVSVIGLGYDLCSDVRFSACKTTPDGSRLVEIDPTRNRDLIFPGGIVVNNVSSSIKCD 75

Query: 67  RGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNAMFGFQSGCWAMDAVNTKCLGLD 126
           +G+RTR +SDIL+FNQMSE FN+   + GKIPSG FN MF F S CW  DA + K L  D
Sbjct: 76  KGERTRLRSDILSFNQMSEKFNQDMCLSGKIPSGMFNNMFAF-SKCWPKDASSVKTLAYD 135

Query: 127 GYFISLFNAHIDRYPLRLSDQVRSAVPSSWDPPAIARFIEKYGTHIIVGLSIGGHDVVLV 186
           G+FISL++  I R  L L D+V+  VPSSWD  A+A FIEKYGTH++VG+++GG DV+ V
Sbjct: 136 GWFISLYSVEIVRKQLTLRDEVKREVPSSWDSAALAGFIEKYGTHVVVGVTMGGKDVIHV 195

Query: 187 RQDKSSTLEPSELKNHLEELGDQLFTGACTFPPPLSKPKDHHKTKAPQAFNLFDPQLVAL 246
           +Q + S  EP E++  L+  GD+ F   C  P     P   +  K P+  NL    L   
Sbjct: 196 KQMRKSNHEPEEIQKMLKHWGDERF---CVDPVESKSPASVYSGK-PKEENLLQWGLQPF 255

Query: 247 GNFSSVSA-----KDGIIVITSKRGGSATASSHCEWLPTVLESPDAIHFQFIPIISLLEG 306
           G   S +       + I+ +  +RGG     SH  WL TV ++P+ I   F+PI SLL G
Sbjct: 256 GTSVSSAVVMHTKNEEIMRVCIRRGGVDLGQSHERWLSTVSQAPNVISMCFVPITSLLSG 315

Query: 307 VPGKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNITTSPSLQLN 366
           +PG GFLSHA+NLYLRYKPPI +L  FL+FQ+ + WAP++ DLPLG   +  +SPSLQ +
Sbjct: 316 LPGTGFLSHAVNLYLRYKPPIEELHQFLEFQLPRQWAPVYGDLPLGLRRSKQSSPSLQFS 375

Query: 367 LMGPKLYVNTTQVTAENKPVTGMRLYLEGIKSNRLAIHIQHLLTTPLLFENNITQQTPPL 426
           LMGPKLYVNT++V +  +PVTG+R +LEG K N LAIH+QHL   P     +      P+
Sbjct: 376 LMGPKLYVNTSKVDSGERPVTGLRFFLEGKKGNHLAIHLQHLSACPPSLHLSHDDTYEPI 435

Query: 427 WYSSQHIADDRYIEPLNRNKFSYVCTAPVKYDPIWATAVAASSSSSNFSPSSSSSPSSPS 486
               +   +  Y  P+    FS+VCT PV+Y+                            
Sbjct: 436 ----EEPVEKGYYVPVKWGIFSHVCTYPVQYN---------------------------- 495

Query: 487 SPTADADSPAAFIVTGAQLHVIKQQDSKTVLHLRLLYSKVSNSCVVQTSWARHSSDISPK 546
              A +D  A+ IVT A L V K    + VL LRL +S  +++   ++ W   S++ S K
Sbjct: 496 --GARSDDTAS-IVTKAWLEV-KGMGMRKVLFLRLGFSLDASAVTRKSCWDNLSTN-SRK 555

Query: 547 SGIFSAISTSLSGAAAVPKEKPDAAAAVIVDSGVFHGGPPVPLQAQKLVKFVELSEMRKG 606
           SG+FS IST LS   +         + + ++S V+  GP  P++  KL+  V+  E+ +G
Sbjct: 556 SGVFSMISTRLSTGLSPNPATTKPQSKIDINSAVYPRGPSPPVK-PKLLSLVDTKEVMRG 608

Query: 607 PQDSPGHWVVIGARLSLEKGKISLHVKFGLLHVVSE 635
           P++ PG+WVV GA+L +E GKIS+  K+ LL V+SE
Sbjct: 616 PEEQPGYWVVTGAKLCVEAGKISIKAKYSLLTVISE 608

BLAST of Spg008663 vs. ExPASy Swiss-Prot
Match: Q9C7N2 (MACPF domain-containing protein CAD1 OS=Arabidopsis thaliana OX=3702 GN=CAD1 PE=2 SV=1)

HSP 1 Score: 406.0 bits (1042), Expect = 8.0e-112
Identity = 243/631 (38.51%), Postives = 340/631 (53.88%), Query Frame = 0

Query: 6   VETALSSLGKGFDLTSDFRLKYCKGE--RRLVLLNEAQRRDLIVPGFGAVQGVSIDIKCD 65
           +  A+ +LG+GFD+TSD RL YCKG    RLV + E Q RDL +     +  V  DI C 
Sbjct: 21  LRNAIQALGRGFDVTSDVRLLYCKGAPGSRLVRIEEGQNRDLELSHGFLLPNVPADIDCS 80

Query: 66  RGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNAMFGFQSGCWAMDAVNTKCLGLD 125
           RG+    +  + +F++M+E FN +  + G IP G FNAMF + +G W +DA +TK L L 
Sbjct: 81  RGNSGTQRISVCSFHEMAEEFNVRSGVKGNIPLGCFNAMFNY-TGSWQVDAASTKSLALV 140

Query: 126 GYFISLFNAHIDRYPLRLSDQVRSAVPSSWDPPAIARFIEKYGTHIIVGLSIGGHDVVLV 185
           GYFI L++  + +  L L +++R AVPSSWDP ++A FIE YGTHI+  ++IGG DVV +
Sbjct: 141 GYFIPLYDVKLAKLTLVLHNEIRRAVPSSWDPASLASFIENYGTHIVTSVTIGGRDVVYI 200

Query: 186 RQDKSSTLEPSELKNHLEELGDQLFTGACTFPPPLSKPKDHHKTKAPQAFNLFDPQLVAL 245
           RQ +SS L  SE++N++ ++                     H+    ++ ++  P     
Sbjct: 201 RQHQSSPLPVSEIENYVNDM-------------------IKHRFHEAESQSITGP----- 260

Query: 246 GNFSSVSAKD-GIIVITSKRGGSATASSHCEWLPTVLESPDAIHFQFIPIISLLEGVPGK 305
                +  KD  I VI  +RGG     SH  W  TV  +PD I+  F PI+SLLEGVPG 
Sbjct: 261 -----LKYKDKDITVIFRRRGGDDLEQSHARWAETVPAAPDIINMTFTPIVSLLEGVPGL 320

Query: 306 GFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNITTSPSLQLNLMGP 365
             L+ AI LYL YKPPI DLQYFLD+Q+ + WAP  ++L            SLQ +LMGP
Sbjct: 321 RHLTRAIELYLEYKPPIEDLQYFLDYQIARAWAPEQSNL----QRKEPVCSSLQFSLMGP 380

Query: 366 KLYVNTTQVTAENKPVTGMRLYLEGIKSNRLAIHIQHLLTTPLLFENNITQQTP---PLW 425
           KL+++  QVT   KPVTG+RL LEG K NRL+IH+QHL++ P + + +     P   P W
Sbjct: 381 KLFISADQVTVGRKPVTGLRLSLEGSKQNRLSIHLQHLVSLPKILQPHWDSHVPIGAPKW 440

Query: 426 YSSQHIADDRYIEPLNRNKFSYVCTAPVKYDPIWATAVAASSSSSNFSPSSSSSPSSPSS 485
              +   D R+ EP+    FS+V T+P+++                             +
Sbjct: 441 QGPEE-QDSRWFEPIKWKNFSHVSTSPIEH-----------------------------T 500

Query: 486 PTADADSPAAFIVTGAQLHVIKQQDSKTVLHLRLLYSKVSNSCVVQTSWARHSSDISPKS 545
            T   D     IVTGAQL V     SK VLHL+LL+SKV    + ++ W           
Sbjct: 501 ETHIGDLSGVHIVTGAQLGV-WDFGSKNVLHLKLLFSKVPGCTIRRSVWDH--------- 560

Query: 546 GIFSAISTSLSGAAAVPKEKPDAAAAVIVDSGVFHGGPPVPLQAQKLVKFVELSEMRKGP 605
                  T ++ +  +    P  +++    SG          Q+ KL K V+ SEM KGP
Sbjct: 561 -------TPVASSGRLEPGGPSTSSSTEEVSG----------QSGKLAKIVDSSEMLKGP 560

Query: 606 QDSPGHWVVIGARLSLEKGKISLHVKFGLLH 631
           QD PGHW+V GA+L +EKGKI L VK+ LL+
Sbjct: 621 QDLPGHWLVTGAKLGVEKGKIVLRVKYSLLN 560

BLAST of Spg008663 vs. ExPASy TrEMBL
Match: A0A6J1JPD7 (MACPF domain-containing protein At1g14780 OS=Cucurbita maxima OX=3661 GN=LOC111487690 PE=4 SV=1)

HSP 1 Score: 1109.7 bits (2869), Expect = 0.0e+00
Identity = 545/640 (85.16%), Postives = 579/640 (90.47%), Query Frame = 0

Query: 1   MGDGIVETALSSLGKGFDLTSDFRLKYCKGERRLVLLNEAQRRDLIVPGFGAVQGVSIDI 60
           MGDGIV+ AL+SLGKGFDLTSDF+LKYCKG+ RLVLLN+AQRRDLIVPGFGAV+ +SIDI
Sbjct: 1   MGDGIVDAALASLGKGFDLTSDFKLKYCKGQHRLVLLNQAQRRDLIVPGFGAVKDISIDI 60

Query: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNAMFGFQSGCWAMDAVNTKCL 120
           KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNAMFGFQSGCWAMDA NTKCL
Sbjct: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNAMFGFQSGCWAMDAENTKCL 120

Query: 121 GLDGYFISLFNAHIDRYPLRLSDQVRSAVPSSWDPPAIARFIEKYGTHIIVGLSIGGHDV 180
           GLDGYFISLFNAHIDRYPLRLSD+VR+AVP+SWDPPAIARFIEKYGTHIIVGLSIGGHDV
Sbjct: 121 GLDGYFISLFNAHIDRYPLRLSDEVRAAVPTSWDPPAIARFIEKYGTHIIVGLSIGGHDV 180

Query: 181 VLVRQDKSSTLEPSELKNHLEELGDQLFTGACTFPPPLSKPKDHHKTKAPQAFNLFDPQL 240
           VLVRQDKSS LEPSELK+HLEELGDQLFTGACTFPPP SKPKDHHKTK PQAFNLFDP+L
Sbjct: 181 VLVRQDKSSNLEPSELKSHLEELGDQLFTGACTFPPPHSKPKDHHKTKLPQAFNLFDPKL 240

Query: 241 VALGNFSSVSAKDGIIVITSKRGGSATASSHCEWLPTVLESPDAIHFQFIPIISLLEGVP 300
           VA  NFSSVSAKDGIIVITSK+GGS TAS+HCEWLPTVLESPDAIHFQFIPIISLL+ +P
Sbjct: 241 VAYANFSSVSAKDGIIVITSKKGGSPTASTHCEWLPTVLESPDAIHFQFIPIISLLDRIP 300

Query: 301 GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNITTSPSLQLNLM 360
           GKGFLSHAINLYLRYKPPIADLQYFLDFQ+HKIWAPIHNDLPLGPTPNITTSP LQ NLM
Sbjct: 301 GKGFLSHAINLYLRYKPPIADLQYFLDFQMHKIWAPIHNDLPLGPTPNITTSPYLQPNLM 360

Query: 361 GPKLYVNTTQVTAENKPVTGMRLYLEGIKSNRLAIHIQHLLTTPLLFENNITQQTPPLWY 420
           GPKLYVNTTQVT+ENKPVTG+RLYLEGIKSNRLAIHIQHL TTP+L +NNITQ  PPLW+
Sbjct: 361 GPKLYVNTTQVTSENKPVTGLRLYLEGIKSNRLAIHIQHLQTTPILLQNNITQHNPPLWH 420

Query: 421 SSQHIADDRYIEPLNRNKFSYVCTAPVKYDPIWATAVAASSSSSNFSPSSSSSPSSPSSP 480
           SS H+ADDRY+EPLNRNKFSYV TAPVKYDP WATA A                    +P
Sbjct: 421 SSLHVADDRYVEPLNRNKFSYVSTAPVKYDPTWATATA--------------------TP 480

Query: 481 TADADSPAAFIVTGAQLHVIKQQDSKTVLHLRLLYSKVSNSCVVQTSWARHSSDISPKSG 540
           T     PAAFIVTGAQLHV+K  DSK  LHL LLYSKVSNS VVQT+WARHSSDISPKSG
Sbjct: 481 T-----PAAFIVTGAQLHVVKHHDSKAALHLGLLYSKVSNSYVVQTNWARHSSDISPKSG 540

Query: 541 IFSAISTSLSGAAAVPKEKPDAAAAVIVDSGVFHGGPPVPLQAQKLVKFVELSEMRKGPQ 600
           IFSAISTSLSG+A+ PK+KP   A VIVDSG+FH GPPVPLQAQKLVKFVELSEMRKGPQ
Sbjct: 541 IFSAISTSLSGSASAPKKKPPDPATVIVDSGIFHHGPPVPLQAQKLVKFVELSEMRKGPQ 600

Query: 601 DSPGHWVVIGARLSLEKGKISLHVKFGLLHVVSETN-DEQ 640
           DSPGHWVVIGARL+LE GKI LHVKFGL+HVVSET+ DEQ
Sbjct: 601 DSPGHWVVIGARLNLENGKICLHVKFGLVHVVSETSGDEQ 615

BLAST of Spg008663 vs. ExPASy TrEMBL
Match: A0A6J1GM16 (MACPF domain-containing protein At1g14780 OS=Cucurbita moschata OX=3662 GN=LOC111455473 PE=4 SV=1)

HSP 1 Score: 1103.2 bits (2852), Expect = 0.0e+00
Identity = 546/640 (85.31%), Postives = 574/640 (89.69%), Query Frame = 0

Query: 1   MGDGIVETALSSLGKGFDLTSDFRLKYCKGERRLVLLNEAQRRDLIVPGFGAVQGVSIDI 60
           MGDGIV+ AL+SLGKGFDLTSDF+LKYCKG+ RLVLLNEAQRRDLIVPGFG V+ VSIDI
Sbjct: 1   MGDGIVDAALASLGKGFDLTSDFKLKYCKGKHRLVLLNEAQRRDLIVPGFGPVKDVSIDI 60

Query: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNAMFGFQSGCWAMDAVNTKCL 120
           KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSG FNAMFGFQSGCWAMDAVNTKCL
Sbjct: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGFFNAMFGFQSGCWAMDAVNTKCL 120

Query: 121 GLDGYFISLFNAHIDRYPLRLSDQVRSAVPSSWDPPAIARFIEKYGTHIIVGLSIGGHDV 180
           GLDGYFISLFNAHIDRYPLRLSD+VR+AVPSSWDPPAIARFIEKYGTHIIVGLSIGGHDV
Sbjct: 121 GLDGYFISLFNAHIDRYPLRLSDEVRAAVPSSWDPPAIARFIEKYGTHIIVGLSIGGHDV 180

Query: 181 VLVRQDKSSTLEPSELKNHLEELGDQLFTGACTFPPPLSKPKDHHKTKAPQAFNLFDPQL 240
           VLVRQDKSS LE SELK+HLEELGDQLFTGACTFPPP SKPKDHHKTK PQAFNLFDP+L
Sbjct: 181 VLVRQDKSSNLEASELKSHLEELGDQLFTGACTFPPPHSKPKDHHKTKLPQAFNLFDPKL 240

Query: 241 VALGNFSSVSAKDGIIVITSKRGGSATASSHCEWLPTVLESPDAIHFQFIPIISLLEGVP 300
           VA  NFSSVSAKDGIIVITSK+GGS TAS+HCEWLPTVLESPDAIHFQFIPIISLL+ VP
Sbjct: 241 VAYANFSSVSAKDGIIVITSKKGGSPTASTHCEWLPTVLESPDAIHFQFIPIISLLDRVP 300

Query: 301 GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNITTSPSLQLNLM 360
           GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNITTSP LQ NLM
Sbjct: 301 GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNITTSPYLQPNLM 360

Query: 361 GPKLYVNTTQVTAENKPVTGMRLYLEGIKSNRLAIHIQHLLTTPLLFENNITQQTPPLWY 420
           GPKLYVNTTQVT+ENKPVTG+RLYLEGIKSNRLAIHIQHL TTP+L +NNITQ  PPLW+
Sbjct: 361 GPKLYVNTTQVTSENKPVTGLRLYLEGIKSNRLAIHIQHLQTTPILLQNNITQHNPPLWH 420

Query: 421 SSQHIADDRYIEPLNRNKFSYVCTAPVKYDPIWATAVAASSSSSNFSPSSSSSPSSPSSP 480
           SS H+ADDRY+EPLNRNKFSYV TAPV YDP WATA                      +P
Sbjct: 421 SSLHVADDRYVEPLNRNKFSYVSTAPVTYDPTWATA----------------------TP 480

Query: 481 TADADSPAAFIVTGAQLHVIKQQDSKTVLHLRLLYSKVSNSCVVQTSWARHSSDISPKSG 540
           T     PAAFIVTGAQLHV+K  DSK  LHL LLYSKVSNS +VQT+WARHSSDISPKSG
Sbjct: 481 T-----PAAFIVTGAQLHVVKHHDSKAALHLGLLYSKVSNSYIVQTNWARHSSDISPKSG 540

Query: 541 IFSAISTSLSGAAAVPKEKPDAAAAVIVDSGVFHGGPPVPLQAQKLVKFVELSEMRKGPQ 600
           IFSAISTSLSGA++ PKEKP   A VIVDSGVFH GPPVPLQAQKLVKFVELSEMRKGPQ
Sbjct: 541 IFSAISTSLSGASSAPKEKPPDPATVIVDSGVFHHGPPVPLQAQKLVKFVELSEMRKGPQ 600

Query: 601 DSPGHWVVIGARLSLEKGKISLHVKFGLLHVVSETN-DEQ 640
           DSPGHWVVIGARL+LE  KI LHVKFGL+HVVSET+ DEQ
Sbjct: 601 DSPGHWVVIGARLNLENSKICLHVKFGLVHVVSETSADEQ 613

BLAST of Spg008663 vs. ExPASy TrEMBL
Match: A0A5D3CRV5 (MACPF domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold663G00330 PE=4 SV=1)

HSP 1 Score: 1100.1 bits (2844), Expect = 0.0e+00
Identity = 547/640 (85.47%), Postives = 577/640 (90.16%), Query Frame = 0

Query: 1   MGDGIVETALSSLGKGFDLTSDFRLKYCKGERRLVLLNEAQRRDLIVPGFGAVQGVSIDI 60
           M D IV+ AL+SLGKGFDLTSDFRLKYCKG   LVLLN++QRRDLIVPGFGAV+ VS+DI
Sbjct: 1   MADEIVQNALNSLGKGFDLTSDFRLKYCKGVHSLVLLNQSQRRDLIVPGFGAVKDVSVDI 60

Query: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNAMFGFQSGCWAMDAVNTKCL 120
           KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFN+MFGFQSGCWA DAVNTKCL
Sbjct: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNSMFGFQSGCWATDAVNTKCL 120

Query: 121 GLDGYFISLFNAHIDRYPLRLSDQVRSAVPSSWDPPAIARFIEKYGTHIIVGLSIGGHDV 180
           GLDGYFISLFNAHIDRYPLRLSD+VRSAVPS+WDPPAIARFIEKYGTHIIVGLSIGGHDV
Sbjct: 121 GLDGYFISLFNAHIDRYPLRLSDEVRSAVPSTWDPPAIARFIEKYGTHIIVGLSIGGHDV 180

Query: 181 VLVRQDKSSTLEPSELKNHLEELGDQLFTGACTFPPPLSKPKDHHKTKAPQAFNLFDPQL 240
           VLVRQDKSS LEPSELKNHLEELGDQLF GACTFPPPLSKPKDHHK K PQAFNLFDPQL
Sbjct: 181 VLVRQDKSSKLEPSELKNHLEELGDQLFNGACTFPPPLSKPKDHHKPKIPQAFNLFDPQL 240

Query: 241 VALGNFSSVSAKDGIIVITSKRGGSATASSHCEWLPTVLESPDAIHFQFIPIISLLEGVP 300
           VA GNFSSVSAKDGIIVITSKRGGS TASSHCEWLPTV ESPDAIHFQFIPIISLLEG+P
Sbjct: 241 VAFGNFSSVSAKDGIIVITSKRGGSPTASSHCEWLPTVHESPDAIHFQFIPIISLLEGIP 300

Query: 301 GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNITTSPSLQLNLM 360
           GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNI+TSPSLQLNLM
Sbjct: 301 GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNISTSPSLQLNLM 360

Query: 361 GPKLYVNTTQVTAENKPVTGMRLYLEGIKSNRLAIHIQHLLTTPLLFENNITQQTPPLWY 420
           GPKLYVNTTQV A+NKPVTGMRLYLEGIKSNRLAIHIQHLLTTPLL ENNITQQT   W+
Sbjct: 361 GPKLYVNTTQVIADNKPVTGMRLYLEGIKSNRLAIHIQHLLTTPLLLENNITQQT--YWH 420

Query: 421 SSQHIADDRYIEPLNRNKFSYVCTAPVKYDPIWATAVAASSSSSNFSPSSSSSPSSPSSP 480
           SSQHIADDRY+EPLNRNKFS+VCT P+KYDP WAT                         
Sbjct: 421 SSQHIADDRYVEPLNRNKFSHVCTVPIKYDPAWATT------------------------ 480

Query: 481 TADADSPAAFIVTGAQLHVIKQQDSKTVLHLRLLYSKVSNSCVVQTSWARHSSDISPKSG 540
           + D +SPAAFIVTGAQL V+KQ DSKTVLHLRLLYSKVSNS +VQT+WARHSSDISPKSG
Sbjct: 481 SGDDNSPAAFIVTGAQLLVVKQHDSKTVLHLRLLYSKVSNSYIVQTNWARHSSDISPKSG 540

Query: 541 IFSAISTSLSGAAAVPKEKPDAAAAVIVDSGVFHGGPPVPLQAQKLVKFVELSEMRKGPQ 600
           IFSAISTSL+GA+A  KEKP   AAVIVDSGVFHGGPP+ +Q QKLVKFVELSEMRKGPQ
Sbjct: 541 IFSAISTSLTGASAALKEKP-PEAAVIVDSGVFHGGPPMSVQVQKLVKFVELSEMRKGPQ 600

Query: 601 DSPGHWVVIGARLSLEKGKISLHVKFGLLHVVSET-NDEQ 640
           DSPG+W+VIGARL+LEKGKI L VKFGLLHV+SET  D+Q
Sbjct: 601 DSPGYWMVIGARLNLEKGKICLQVKFGLLHVLSETITDDQ 613

BLAST of Spg008663 vs. ExPASy TrEMBL
Match: E5GB62 (MACPF domain-containing protein OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 1100.1 bits (2844), Expect = 0.0e+00
Identity = 547/640 (85.47%), Postives = 577/640 (90.16%), Query Frame = 0

Query: 1   MGDGIVETALSSLGKGFDLTSDFRLKYCKGERRLVLLNEAQRRDLIVPGFGAVQGVSIDI 60
           M D IV+ AL+SLGKGFDLTSDFRLKYCKG   LVLLN++QRRDLIVPGFGAV+ VS+DI
Sbjct: 1   MADEIVQNALNSLGKGFDLTSDFRLKYCKGVHSLVLLNQSQRRDLIVPGFGAVKDVSVDI 60

Query: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNAMFGFQSGCWAMDAVNTKCL 120
           KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFN+MFGFQSGCWA DAVNTKCL
Sbjct: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNSMFGFQSGCWATDAVNTKCL 120

Query: 121 GLDGYFISLFNAHIDRYPLRLSDQVRSAVPSSWDPPAIARFIEKYGTHIIVGLSIGGHDV 180
           GLDGYFISLFNAHIDRYPLRLSD+VRSAVPS+WDPPAIARFIEKYGTHIIVGLSIGGHDV
Sbjct: 121 GLDGYFISLFNAHIDRYPLRLSDEVRSAVPSTWDPPAIARFIEKYGTHIIVGLSIGGHDV 180

Query: 181 VLVRQDKSSTLEPSELKNHLEELGDQLFTGACTFPPPLSKPKDHHKTKAPQAFNLFDPQL 240
           VLVRQDKSS LEPSELKNHLEELGDQLF GACTFPPPLSKPKDHHK K PQAFNLFDPQL
Sbjct: 181 VLVRQDKSSKLEPSELKNHLEELGDQLFNGACTFPPPLSKPKDHHKPKIPQAFNLFDPQL 240

Query: 241 VALGNFSSVSAKDGIIVITSKRGGSATASSHCEWLPTVLESPDAIHFQFIPIISLLEGVP 300
           VA GNFSSVSAKDGIIVITSKRGGS TASSHCEWLPTV ESPDAIHFQFIPIISLLEG+P
Sbjct: 241 VAFGNFSSVSAKDGIIVITSKRGGSPTASSHCEWLPTVHESPDAIHFQFIPIISLLEGIP 300

Query: 301 GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNITTSPSLQLNLM 360
           GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNI+TSPSLQLNLM
Sbjct: 301 GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNISTSPSLQLNLM 360

Query: 361 GPKLYVNTTQVTAENKPVTGMRLYLEGIKSNRLAIHIQHLLTTPLLFENNITQQTPPLWY 420
           GPKLYVNTTQV A+NKPVTGMRLYLEGIKSNRLAIHIQHLLTTPLL ENNITQQT   W+
Sbjct: 361 GPKLYVNTTQVIADNKPVTGMRLYLEGIKSNRLAIHIQHLLTTPLLLENNITQQT--YWH 420

Query: 421 SSQHIADDRYIEPLNRNKFSYVCTAPVKYDPIWATAVAASSSSSNFSPSSSSSPSSPSSP 480
           SSQHIADDRY+EPLNRNKFS+VCT P+KYDP WAT                         
Sbjct: 421 SSQHIADDRYVEPLNRNKFSHVCTVPIKYDPAWATT------------------------ 480

Query: 481 TADADSPAAFIVTGAQLHVIKQQDSKTVLHLRLLYSKVSNSCVVQTSWARHSSDISPKSG 540
           + D +SPAAFIVTGAQL V+KQ DSKTVLHLRLLYSKVSNS +VQT+WARHSSDISPKSG
Sbjct: 481 SGDDNSPAAFIVTGAQLLVVKQHDSKTVLHLRLLYSKVSNSYIVQTNWARHSSDISPKSG 540

Query: 541 IFSAISTSLSGAAAVPKEKPDAAAAVIVDSGVFHGGPPVPLQAQKLVKFVELSEMRKGPQ 600
           IFSAISTSL+GA+A  KEKP   AAVIVDSGVFHGGPP+ +Q QKLVKFVELSEMRKGPQ
Sbjct: 541 IFSAISTSLTGASAALKEKP-PEAAVIVDSGVFHGGPPMSVQVQKLVKFVELSEMRKGPQ 600

Query: 601 DSPGHWVVIGARLSLEKGKISLHVKFGLLHVVSET-NDEQ 640
           DSPG+W+VIGARL+LEKGKI L VKFGLLHV+SET  D+Q
Sbjct: 601 DSPGYWMVIGARLNLEKGKICLQVKFGLLHVLSETITDDQ 613

BLAST of Spg008663 vs. ExPASy TrEMBL
Match: A0A1S3CSS8 (LOW QUALITY PROTEIN: MACPF domain-containing protein At1g14780-like OS=Cucumis melo OX=3656 GN=LOC103504476 PE=4 SV=1)

HSP 1 Score: 1097.8 bits (2838), Expect = 0.0e+00
Identity = 546/640 (85.31%), Postives = 576/640 (90.00%), Query Frame = 0

Query: 1   MGDGIVETALSSLGKGFDLTSDFRLKYCKGERRLVLLNEAQRRDLIVPGFGAVQGVSIDI 60
           M D IV+ AL+SLGKGFDLTSDFRLKYCKG   LVLLN++QRRDLIVPGFGAV+ VS+DI
Sbjct: 1   MADEIVQNALNSLGKGFDLTSDFRLKYCKGVHSLVLLNQSQRRDLIVPGFGAVKDVSVDI 60

Query: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNAMFGFQSGCWAMDAVNTKCL 120
           KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFN+MFGFQSGCWA DAVNTKCL
Sbjct: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNSMFGFQSGCWATDAVNTKCL 120

Query: 121 GLDGYFISLFNAHIDRYPLRLSDQVRSAVPSSWDPPAIARFIEKYGTHIIVGLSIGGHDV 180
           GLDGYFISLFNAHIDRYPLRLSD+VRSAVPS+WDPPAIARFIEKYGTHIIVGLSIGGHDV
Sbjct: 121 GLDGYFISLFNAHIDRYPLRLSDEVRSAVPSTWDPPAIARFIEKYGTHIIVGLSIGGHDV 180

Query: 181 VLVRQDKSSTLEPSELKNHLEELGDQLFTGACTFPPPLSKPKDHHKTKAPQAFNLFDPQL 240
           VLVRQDKSS LEPSELKNHLEELGDQLF GACTFPPPLSKPKDHHK K PQAFNLFDPQL
Sbjct: 181 VLVRQDKSSKLEPSELKNHLEELGDQLFNGACTFPPPLSKPKDHHKPKIPQAFNLFDPQL 240

Query: 241 VALGNFSSVSAKDGIIVITSKRGGSATASSHCEWLPTVLESPDAIHFQFIPIISLLEGVP 300
           VA GNFSSVSAKDGIIVITSKRGGS TASSHCEWLPTV ESPDAIHFQFIPIISLLEG+P
Sbjct: 241 VAFGNFSSVSAKDGIIVITSKRGGSPTASSHCEWLPTVHESPDAIHFQFIPIISLLEGIP 300

Query: 301 GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNITTSPSLQLNLM 360
           GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNI+TSPSLQLNLM
Sbjct: 301 GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNISTSPSLQLNLM 360

Query: 361 GPKLYVNTTQVTAENKPVTGMRLYLEGIKSNRLAIHIQHLLTTPLLFENNITQQTPPLWY 420
           GPKLYVNTTQV A+NKPVTGMRLYLEGIKSNRLAIHIQHLLTTPLL ENNITQQT   W+
Sbjct: 361 GPKLYVNTTQVIADNKPVTGMRLYLEGIKSNRLAIHIQHLLTTPLLLENNITQQT--YWH 420

Query: 421 SSQHIADDRYIEPLNRNKFSYVCTAPVKYDPIWATAVAASSSSSNFSPSSSSSPSSPSSP 480
           SSQHIADDRY+EPLNRNKFS+VCT P+KYDP WAT                         
Sbjct: 421 SSQHIADDRYVEPLNRNKFSHVCTVPIKYDPAWATT------------------------ 480

Query: 481 TADADSPAAFIVTGAQLHVIKQQDSKTVLHLRLLYSKVSNSCVVQTSWARHSSDISPKSG 540
           + D +SPAAFIVTGAQL V+KQ DSK VLHLRLLYSKVSNS +VQT+WARHSSDISPKSG
Sbjct: 481 SGDDNSPAAFIVTGAQLLVVKQHDSKXVLHLRLLYSKVSNSYIVQTNWARHSSDISPKSG 540

Query: 541 IFSAISTSLSGAAAVPKEKPDAAAAVIVDSGVFHGGPPVPLQAQKLVKFVELSEMRKGPQ 600
           IFSAISTSL+GA+A  KEKP   AAVIVDSGVFHGGPP+ +Q QKLVKFVELSEMRKGPQ
Sbjct: 541 IFSAISTSLTGASAALKEKP-PEAAVIVDSGVFHGGPPMSVQVQKLVKFVELSEMRKGPQ 600

Query: 601 DSPGHWVVIGARLSLEKGKISLHVKFGLLHVVSET-NDEQ 640
           DSPG+W+VIGARL+LEKGKI L VKFGLLHV+SET  D+Q
Sbjct: 601 DSPGYWMVIGARLNLEKGKICLQVKFGLLHVLSETITDDQ 613

BLAST of Spg008663 vs. TAIR 10
Match: AT1G14780.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 685.6 bits (1768), Expect = 3.7e-197
Identity = 360/646 (55.73%), Postives = 449/646 (69.50%), Query Frame = 0

Query: 2   GDGIVETALSSLGKGFDLTSDFRLKYCK------GERRLVLLNEAQRRDLIVPGFGAVQG 61
           G  ++ETA+ SLGKGFDLT+DFRLKYCK      G+ RLV+L++ Q R+L +PGFG  Q 
Sbjct: 5   GGDVIETAVKSLGKGFDLTADFRLKYCKDGDGSAGDDRLVVLDQTQNRELHIPGFGVFQN 64

Query: 62  VSIDIKCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNAMFGFQSGCWAMDAV 121
           VS DI CD+G+RTR++SDIL+FN+MSE FN++ S+ GKIPSG+FNA FGFQSG WA DA 
Sbjct: 65  VSADINCDKGERTRFRSDILDFNKMSEYFNQRSSVTGKIPSGNFNATFGFQSGSWATDAA 124

Query: 122 NTKCLGLDGYFISLFNAHI-DRYPLRLSDQVRSAVPSSWDPPAIARFIEKYGTHIIVGLS 181
           N K LGLD   ++LFN HI +   LRL+D+VR+AVPSSWDP  +ARFIE+YGTH+I G+S
Sbjct: 125 NVKSLGLDASVVTLFNLHIHNPNRLRLTDRVRNAVPSSWDPQLLARFIERYGTHVITGVS 184

Query: 182 IGGHDVVLVRQDKSSTLEPSELKNHLEELGDQLFTGACTFPPPLSKPKDHH---KTKAPQ 241
           +GG DVV+VRQDKSS L+   L++HL +LGDQLFTG+C           HH   + K P+
Sbjct: 185 VGGQDVVVVRQDKSSDLDNDLLRHHLYDLGDQLFTGSCLLSTRRLNKAYHHSHSQPKFPE 244

Query: 242 AFNLF-DPQLVALGNFSSVSAKDGIIVITSKRGGSATASSHCEWLPTVLESPDAIHFQFI 301
           AFN+F D Q VA  NF S+++++GI VI +KRGG   A SH EWL TV + PDAI+F FI
Sbjct: 245 AFNVFDDKQTVAFNNF-SINSQNGITVICAKRGGDGRAKSHSEWLITVPDKPDAINFNFI 304

Query: 302 PIISLLEGVPGKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNIT 361
           PI SLL+ VPG G LSHA++LYLRYKPP+ DLQYFLDF   + WAP+HNDLP G  PN+ 
Sbjct: 305 PITSLLKDVPGSGLLSHAMSLYLRYKPPLMDLQYFLDFSGPRAWAPVHNDLPFGAAPNMA 364

Query: 362 TS-PSLQLNLMGPKLYVNTTQVTAENKPVTGMRLYLEGIKSNRLAIHIQHLLTTPLLFEN 421
           ++ P+L +N MGPKLYVNTT VT+E  PVTGMR +LEG K NRLAIH+QHL  T      
Sbjct: 365 SAYPALHINFMGPKLYVNTTPVTSEKNPVTGMRFFLEGKKCNRLAIHLQHLDNTRTTVGE 424

Query: 422 NITQQTPPLWYSSQHIAD-DRYIEPLNRNKFSYVCTAPVKYDPIWATAVAASSSSSNFSP 481
            IT +   +W  S  I D DRY EPLN  KFS+VCT PVKYDP W    +   S ++   
Sbjct: 425 KITDE--HIWRGSDQITDNDRYFEPLNGKKFSHVCTVPVKYDPNWIKTTSNHKSQND--- 484

Query: 482 SSSSSPSSPSSPTADADSPAAFIVTGAQLHVIKQQDSKTVLHLRLLYSKVSNSCVVQTSW 541
                               AFIVTGAQL V K+  SK+VLHLRL Y+KVS+  VVQ SW
Sbjct: 485 -------------------VAFIVTGAQLEV-KKHGSKSVLHLRLRYTKVSDHYVVQNSW 544

Query: 542 ARHSSDISPKSGIFSAISTSLSGAAA----VPKEKPDAAAAVIVDSGVFHGGPPVPLQAQ 601
                  S KSGIFS++S  L+  +     + K+K +    V++DSGVF GGPPVP    
Sbjct: 545 VHGPIGTSQKSGIFSSMSMPLTSGSVHHNMIQKDKNE----VVLDSGVFPGGPPVPAN-N 604

Query: 602 KLVKFVELSEMRKGPQDSPGHWVVIGARLSLEKGKISLHVKFGLLH 631
           K+VKFV+LS++ +GPQ SPGHW+V G RL L+KGK+ LHVKF LLH
Sbjct: 605 KIVKFVDLSQLCRGPQHSPGHWLVTGVRLYLDKGKLCLHVKFALLH 619

BLAST of Spg008663 vs. TAIR 10
Match: AT4G24290.2 (MAC/Perforin domain-containing protein )

HSP 1 Score: 499.2 bits (1284), Expect = 4.9e-141
Identity = 281/643 (43.70%), Postives = 385/643 (59.88%), Query Frame = 0

Query: 7   ETALSSLGKGFDLTSDFRLKYCKG---ERRLVLLNEAQRR-DLIVPGFGAVQGVSIDIKC 66
           E A+ S+G G+DL  D RLKYCKG   + RL+ + E     ++++PG  ++  VS  IKC
Sbjct: 12  EVAIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGISIPNVSKSIKC 71

Query: 67  DRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNAMFGFQSGCWAMDAVNTKCLGL 126
           D+G+R R++SDIL F QM+E FN++ S+ GKIPSG FNAMF F S CW  DA  TK L  
Sbjct: 72  DKGERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEF-SSCWQKDAAYTKNLAF 131

Query: 127 DGYFISLFNAHIDRYPLRLSDQVRSAVPSSWDPPAIARFIEKYGTHIIVGLSIGGHDVVL 186
           DG FISL++  +D+  + L + V+ AVPS+WDP A+ARFI+ YGTHIIV + +GG DV+ 
Sbjct: 132 DGVFISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVSVKMGGKDVIY 191

Query: 187 VRQDKSSTLEPSELKNHLEELGDQLFTGACTFPPPLSKPKDHHKTKAPQAFNLFDPQLVA 246
            +Q  SS L+P +L+  L+E+ D+ F  A       S+          +   L      +
Sbjct: 192 AKQQHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVETKEQRLRFADTSS 251

Query: 247 LGNFSSVSAKDGIIVITSKRGGSATAS-SHCEWLPTVLESPDAIHFQFIPIISLLEGVPG 306
           LG++++   K+  + +  +RGG+   +  H EWL TV   PD I   FIPI SLL GVPG
Sbjct: 252 LGSYAN---KEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFIPITSLLNGVPG 311

Query: 307 KGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNITTSPSLQLNLMG 366
            GFLSHAINLYLRYKPPI +L  FL+FQ+ + WAP+ ++LPLGP     +  SLQ +  G
Sbjct: 312 SGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPVFSELPLGPQRKQQSCASLQFSFFG 371

Query: 367 PKLYVNTTQVTAENKPVTGMRLYLEGIKSNRLAIHIQHLLTTPLLF--ENNITQQTPPLW 426
           PKLYVNTT V    +P+TGMRLYLEG +SNRLAIH+QHL + P ++  E+++ +      
Sbjct: 372 PKLYVNTTPVDVGKRPITGMRLYLEGRRSNRLAIHLQHLSSLPKIYQLEDDLNRSI---- 431

Query: 427 YSSQHIADDRYIEPLNRNKFSYVCTAPVKYDPIWATAVAASSSSSNFSPSSSSSPSSPSS 486
              Q   D RY E +N   +S+VCT PV+ D   +                         
Sbjct: 432 --RQESHDRRYYEKVNWKNYSHVCTEPVESDDDLS------------------------- 491

Query: 487 PTADADSPAAFIVTGAQLHVIKQQDSKTVLHLRLLYSKVSNSCVVQTSWARHSSDISPKS 546
                      +VTGAQLHV +    K VL LRL +S+V  + +V+ S    +   +PKS
Sbjct: 492 -----------VVTGAQLHV-ESHGFKNVLFLRLCFSRVVGATLVKNSEWDEAVGFAPKS 551

Query: 547 GIFSAISTSLSGAAAVPKEKPDAAAAVIVDSGVFHGGPPVPLQAQKLVKFVELSEMRKGP 606
           G+ S + +    AA  P  +P   A V ++S ++ GGPPVP QA KL+KFV+ SEM +GP
Sbjct: 552 GLISTLISHHFTAAQKPPPRP---ADVNINSAIYPGGPPVPTQAPKLLKFVDTSEMTRGP 604

Query: 607 QDSPGHWVVIGARLSLEKGKISLHVKFGLLHVV--SETNDEQY 641
           Q+SPG+WVV GARL +EKGKISL VK+ L   +   E  +E Y
Sbjct: 612 QESPGYWVVSGARLLVEKGKISLKVKYSLFTPILGDEVIEEAY 604

BLAST of Spg008663 vs. TAIR 10
Match: AT1G28380.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 459.9 bits (1182), Expect = 3.3e-129
Identity = 270/636 (42.45%), Postives = 370/636 (58.18%), Query Frame = 0

Query: 7   ETALSSLGKGFDLTSDFRLKYCK---GERRLVLLNEAQRRDLIVPGFGAVQGVSIDIKCD 66
           E A+S +G G+DL SD R   CK      RLV ++  + RDLI PG   V  VS  IKCD
Sbjct: 16  EKAVSVIGLGYDLCSDVRFSACKTTPDGSRLVEIDPTRNRDLIFPGGIVVNNVSSSIKCD 75

Query: 67  RGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNAMFGFQSGCWAMDAVNTKCLGLD 126
           +G+RTR +SDIL+FNQMSE FN+   + GKIPSG FN MF F S CW  DA + K L  D
Sbjct: 76  KGERTRLRSDILSFNQMSEKFNQDMCLSGKIPSGMFNNMFAF-SKCWPKDASSVKTLAYD 135

Query: 127 GYFISLFNAHIDRYPLRLSDQVRSAVPSSWDPPAIARFIEKYGTHIIVGLSIGGHDVVLV 186
           G+FISL++  I R  L L D+V+  VPSSWD  A+A FIEKYGTH++VG+++GG DV+ V
Sbjct: 136 GWFISLYSVEIVRKQLTLRDEVKREVPSSWDSAALAGFIEKYGTHVVVGVTMGGKDVIHV 195

Query: 187 RQDKSSTLEPSELKNHLEELGDQLFTGACTFPPPLSKPKDHHKTKAPQAFNLFDPQLVAL 246
           +Q + S  EP E++  L+  GD+ F   C  P     P   +  K P+  NL    L   
Sbjct: 196 KQMRKSNHEPEEIQKMLKHWGDERF---CVDPVESKSPASVYSGK-PKEENLLQWGLQPF 255

Query: 247 GNFSSVSA-----KDGIIVITSKRGGSATASSHCEWLPTVLESPDAIHFQFIPIISLLEG 306
           G   S +       + I+ +  +RGG     SH  WL TV ++P+ I   F+PI SLL G
Sbjct: 256 GTSVSSAVVMHTKNEEIMRVCIRRGGVDLGQSHERWLSTVSQAPNVISMCFVPITSLLSG 315

Query: 307 VPGKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNITTSPSLQLN 366
           +PG GFLSHA+NLYLRYKPPI +L  FL+FQ+ + WAP++ DLPLG   +  +SPSLQ +
Sbjct: 316 LPGTGFLSHAVNLYLRYKPPIEELHQFLEFQLPRQWAPVYGDLPLGLRRSKQSSPSLQFS 375

Query: 367 LMGPKLYVNTTQVTAENKPVTGMRLYLEGIKSNRLAIHIQHLLTTPLLFENNITQQTPPL 426
           LMGPKLYVNT++V +  +PVTG+R +LEG K N LAIH+QHL   P     +      P+
Sbjct: 376 LMGPKLYVNTSKVDSGERPVTGLRFFLEGKKGNHLAIHLQHLSACPPSLHLSHDDTYEPI 435

Query: 427 WYSSQHIADDRYIEPLNRNKFSYVCTAPVKYDPIWATAVAASSSSSNFSPSSSSSPSSPS 486
               +   +  Y  P+    FS+VCT PV+Y+                            
Sbjct: 436 ----EEPVEKGYYVPVKWGIFSHVCTYPVQYN---------------------------- 495

Query: 487 SPTADADSPAAFIVTGAQLHVIKQQDSKTVLHLRLLYSKVSNSCVVQTSWARHSSDISPK 546
              A +D  A+ IVT A L V K    + VL LRL +S  +++   ++ W   S++ S K
Sbjct: 496 --GARSDDTAS-IVTKAWLEV-KGMGMRKVLFLRLGFSLDASAVTRKSCWDNLSTN-SRK 555

Query: 547 SGIFSAISTSLSGAAAVPKEKPDAAAAVIVDSGVFHGGPPVPLQAQKLVKFVELSEMRKG 606
           SG+FS IST LS   +         + + ++S V+  GP  P++  KL+  V+  E+ +G
Sbjct: 556 SGVFSMISTRLSTGLSPNPATTKPQSKIDINSAVYPRGPSPPVK-PKLLSLVDTKEVMRG 608

Query: 607 PQDSPGHWVVIGARLSLEKGKISLHVKFGLLHVVSE 635
           P++ PG+WVV GA+L +E GKIS+  K+ LL V+SE
Sbjct: 616 PEEQPGYWVVTGAKLCVEAGKISIKAKYSLLTVISE 608

BLAST of Spg008663 vs. TAIR 10
Match: AT1G29690.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 406.0 bits (1042), Expect = 5.7e-113
Identity = 243/631 (38.51%), Postives = 340/631 (53.88%), Query Frame = 0

Query: 6   VETALSSLGKGFDLTSDFRLKYCKGE--RRLVLLNEAQRRDLIVPGFGAVQGVSIDIKCD 65
           +  A+ +LG+GFD+TSD RL YCKG    RLV + E Q RDL +     +  V  DI C 
Sbjct: 21  LRNAIQALGRGFDVTSDVRLLYCKGAPGSRLVRIEEGQNRDLELSHGFLLPNVPADIDCS 80

Query: 66  RGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNAMFGFQSGCWAMDAVNTKCLGLD 125
           RG+    +  + +F++M+E FN +  + G IP G FNAMF + +G W +DA +TK L L 
Sbjct: 81  RGNSGTQRISVCSFHEMAEEFNVRSGVKGNIPLGCFNAMFNY-TGSWQVDAASTKSLALV 140

Query: 126 GYFISLFNAHIDRYPLRLSDQVRSAVPSSWDPPAIARFIEKYGTHIIVGLSIGGHDVVLV 185
           GYFI L++  + +  L L +++R AVPSSWDP ++A FIE YGTHI+  ++IGG DVV +
Sbjct: 141 GYFIPLYDVKLAKLTLVLHNEIRRAVPSSWDPASLASFIENYGTHIVTSVTIGGRDVVYI 200

Query: 186 RQDKSSTLEPSELKNHLEELGDQLFTGACTFPPPLSKPKDHHKTKAPQAFNLFDPQLVAL 245
           RQ +SS L  SE++N++ ++                     H+    ++ ++  P     
Sbjct: 201 RQHQSSPLPVSEIENYVNDM-------------------IKHRFHEAESQSITGP----- 260

Query: 246 GNFSSVSAKD-GIIVITSKRGGSATASSHCEWLPTVLESPDAIHFQFIPIISLLEGVPGK 305
                +  KD  I VI  +RGG     SH  W  TV  +PD I+  F PI+SLLEGVPG 
Sbjct: 261 -----LKYKDKDITVIFRRRGGDDLEQSHARWAETVPAAPDIINMTFTPIVSLLEGVPGL 320

Query: 306 GFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNITTSPSLQLNLMGP 365
             L+ AI LYL YKPPI DLQYFLD+Q+ + WAP  ++L            SLQ +LMGP
Sbjct: 321 RHLTRAIELYLEYKPPIEDLQYFLDYQIARAWAPEQSNL----QRKEPVCSSLQFSLMGP 380

Query: 366 KLYVNTTQVTAENKPVTGMRLYLEGIKSNRLAIHIQHLLTTPLLFENNITQQTP---PLW 425
           KL+++  QVT   KPVTG+RL LEG K NRL+IH+QHL++ P + + +     P   P W
Sbjct: 381 KLFISADQVTVGRKPVTGLRLSLEGSKQNRLSIHLQHLVSLPKILQPHWDSHVPIGAPKW 440

Query: 426 YSSQHIADDRYIEPLNRNKFSYVCTAPVKYDPIWATAVAASSSSSNFSPSSSSSPSSPSS 485
              +   D R+ EP+    FS+V T+P+++                             +
Sbjct: 441 QGPEE-QDSRWFEPIKWKNFSHVSTSPIEH-----------------------------T 500

Query: 486 PTADADSPAAFIVTGAQLHVIKQQDSKTVLHLRLLYSKVSNSCVVQTSWARHSSDISPKS 545
            T   D     IVTGAQL V     SK VLHL+LL+SKV    + ++ W           
Sbjct: 501 ETHIGDLSGVHIVTGAQLGV-WDFGSKNVLHLKLLFSKVPGCTIRRSVWDH--------- 560

Query: 546 GIFSAISTSLSGAAAVPKEKPDAAAAVIVDSGVFHGGPPVPLQAQKLVKFVELSEMRKGP 605
                  T ++ +  +    P  +++    SG          Q+ KL K V+ SEM KGP
Sbjct: 561 -------TPVASSGRLEPGGPSTSSSTEEVSG----------QSGKLAKIVDSSEMLKGP 560

Query: 606 QDSPGHWVVIGARLSLEKGKISLHVKFGLLH 631
           QD PGHW+V GA+L +EKGKI L VK+ LL+
Sbjct: 621 QDLPGHWLVTGAKLGVEKGKIVLRVKYSLLN 560

BLAST of Spg008663 vs. TAIR 10
Match: AT4G24290.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 275.8 bits (704), Expect = 8.8e-74
Identity = 145/316 (45.89%), Postives = 198/316 (62.66%), Query Frame = 0

Query: 7   ETALSSLGKGFDLTSDFRLKYCKG---ERRLVLLNEAQRR-DLIVPGFGAVQGVSIDIKC 66
           E A+ S+G G+DL  D RLKYCKG   + RL+ + E     ++++PG  ++  VS  IKC
Sbjct: 12  EVAIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGISIPNVSKSIKC 71

Query: 67  DRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNAMFGFQSGCWAMDAVNTKCLGL 126
           D+G+R R++SDIL F QM+E FN++ S+ GKIPSG FNAMF F S CW  DA  TK L  
Sbjct: 72  DKGERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEF-SSCWQKDAAYTKNLAF 131

Query: 127 DGYFISLFNAHIDRYPLRLSDQVRSAVPSSWDPPAIARFIEKYGTHIIVGLSIGGHDVVL 186
           DG FISL++  +D+  + L + V+ AVPS+WDP A+ARFI+ YGTHIIV + +GG DV+ 
Sbjct: 132 DGVFISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVSVKMGGKDVIY 191

Query: 187 VRQDKSSTLEPSELKNHLEELGDQLFTGACTFPPPLSKPKDHHKTKAPQAFNLFDPQLVA 246
            +Q  SS L+P +L+  L+E+ D+ F  A       S+          +   L      +
Sbjct: 192 AKQQHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVETKEQRLRFADTSS 251

Query: 247 LGNFSSVSAKDGIIVITSKRGGSATAS-SHCEWLPTVLESPDAIHFQFIPIISLLEGVPG 306
           LG++++   K+  + +  +RGG+   +  H EWL TV   PD I   FIPI SLL GVPG
Sbjct: 252 LGSYAN---KEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFIPITSLLNGVPG 311

Query: 307 KGFLSHAINLYLRYKP 318
            GFLSHAINLYLR KP
Sbjct: 312 SGFLSHAINLYLRCKP 323