Spg007135 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg007135 vs. NCBI nr
Match: XP_038898390.1 (ent-kaurene oxidase [Benincasa hispida])

HSP 1 Score: 889.4 bits (2297), Expect = 1.4e-254
Identity = 439/517 (84.91%), Postives = 475/517 (91.88%), Query Frame = 0

Query: 1   MAIVNDPLGSMQHLADALQAPPYAAAAVLGGVSLLVSMFLLGDWRKRRRNLLPPVPAIPG 60
           MA+V DPL SMQ LA+ LQAPPYAAAAVLGGVSL++S+FL+ D RK+RRN LPPVPAIPG
Sbjct: 1   MAVVTDPLTSMQLLANTLQAPPYAAAAVLGGVSLVLSVFLVADCRKKRRNFLPPVPAIPG 60

Query: 61  VPVLGNLLQLKEKKPHKTFAQWAETYGPVYSIRTGASTVVVLNTAEVAKEALVTRYSSMS 120
           VPVLGNLLQLKEKKPH TFA+WAE YGPVYSIRTGASTV+VLNTAEVAKEA+VTRY+S+S
Sbjct: 61  VPVLGNLLQLKEKKPHMTFARWAEKYGPVYSIRTGASTVIVLNTAEVAKEAMVTRYASIS 120

Query: 121 SRKLSKALTILTADKCMVAMSDYNDFHKLAKRYILANLLGVNAQKRLRQRRDTMMENICS 180
           SRKLSKALTILTADKCMVAMSDYNDFHK+ KRYILAN+LG NAQK+ RQRRDTM+ENI  
Sbjct: 121 SRKLSKALTILTADKCMVAMSDYNDFHKMVKRYILANMLGANAQKKHRQRRDTMIENISR 180

Query: 181 QLFAQVKDSPSEFVNFRKIFEAELFRLALKETFGKDIESLYVDGLGTTLPREDLFKVLVI 240
           +LFA VK+ PSE VNFRKIFE+ELFRLALKET GKDIES+YVDGLGTTLPREDLF+VLVI
Sbjct: 181 ELFAHVKEFPSESVNFRKIFESELFRLALKETLGKDIESIYVDGLGTTLPREDLFRVLVI 240

Query: 241 DPMEGAIEVDWRDFFPYLRWIPNKGLENKIKQMDFRRRMTMKSLIEDQKKRIAAGEETNC 300
           DPMEGAIEVDWRDFFPYLRWIPNK +ENKI++MDF+R+MTM+SLIE+QKKRIAAGEETNC
Sbjct: 241 DPMEGAIEVDWRDFFPYLRWIPNKSVENKIRKMDFQRQMTMRSLIEEQKKRIAAGEETNC 300

Query: 301 YADFLLSEAKTLTEDQISMLLWETIIETSDTTLV----------------DYLYQHIQSV 360
           YADFLLSEAKTLTEDQISMLLWETIIETSDTTLV                D+LYQHI S 
Sbjct: 301 YADFLLSEAKTLTEDQISMLLWETIIETSDTTLVVTEWAMYELAKDPKRQDFLYQHILSA 360

Query: 361 CGSTKLTEDNLSQLPYLTAVFHETLRKHSPVPIVPLRYAHEDTQLGGFFVPAGSEIAVNI 420
           CGS+KLTE+NLSQLPYLTAVFHETLRKHSPVP+VPLRYAHEDTQLGGFFVPAGSEIAVNI
Sbjct: 361 CGSSKLTEENLSQLPYLTAVFHETLRKHSPVPVVPLRYAHEDTQLGGFFVPAGSEIAVNI 420

Query: 421 YACNMDKQHWESPEEWMPERFLDEKSDPMDLHKTMAFGGGKRVCAGALKAMLISCTTIGK 480
           YACNMDK HWESPEEW PERFLDEK DPMDLHKTMAFGGGKRVCAGALKAMLI+CTTIG+
Sbjct: 421 YACNMDKDHWESPEEWKPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKAMLIACTTIGR 480

Query: 481 LVQEFEWKLREGEEDKVDTLGLTARKLQPLHAIVKPR 502
           + QEFEWKLREGEEDKVDTLGLTARKLQPLH +VKPR
Sbjct: 481 MAQEFEWKLREGEEDKVDTLGLTARKLQPLHVVVKPR 517

BLAST of Spg007135 vs. NCBI nr
Match: KAG7024228.1 (Ent-kaurene oxidase, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 889.0 bits (2296), Expect = 1.9e-254
Identity = 436/501 (87.03%), Postives = 470/501 (93.81%), Query Frame = 0

Query: 1   MAIVNDPLGSMQHLADALQAPPYAAAAVLGGVSLLVSMFLLGDWRKRRRNLLPPVPAIPG 60
           MAI NDPLGS+QHL D LQAP YAAA++LG +S L++ FL+ DWRK RRN LPPVPAIPG
Sbjct: 1   MAIFNDPLGSLQHLLDTLQAPTYAAASILGVISFLLAGFLVADWRKSRRNFLPPVPAIPG 60

Query: 61  VPVLGNLLQLKEKKPHKTFAQWAETYGPVYSIRTGASTVVVLNTAEVAKEALVTRYSSMS 120
           +PVLGNLLQLKEKKPHKTFA+WAETYGP+YSIRTGASTV+VLNTAEVAKEA+VTRY+SMS
Sbjct: 61  LPVLGNLLQLKEKKPHKTFARWAETYGPIYSIRTGASTVIVLNTAEVAKEAMVTRYASMS 120

Query: 121 SRKLSKALTILTADKCMVAMSDYNDFHKLAKRYILANLLGVNAQKRLRQRRDTMMENICS 180
           SRKLSKALTILT+DKCMVAMSDYNDFHK+ K+YILAN+LG NAQKR RQRRDTM++NI S
Sbjct: 121 SRKLSKALTILTSDKCMVAMSDYNDFHKMVKKYILANVLGANAQKRHRQRRDTMIDNISS 180

Query: 181 QLFAQVKDSPSEFVNFRKIFEAELFRLALKETFGKDIESLYVDGLGTTLPREDLFKVLVI 240
           QLFA VKDSPSE VNFR+IFE+ELFRLALKET GKDIES+YVDGLG TLPREDLF+VLVI
Sbjct: 181 QLFAHVKDSPSESVNFRRIFESELFRLALKETLGKDIESIYVDGLG-TLPREDLFRVLVI 240

Query: 241 DPMEGAIEVDWRDFFPYLRWIPNKGLENKIKQMDFRRRMTMKSLIEDQKKRIAAGEETNC 300
           DPMEGAIEVDWRDFFPYL+WIPNK +ENKI+QMDF RR+TM SLIE+QKKRIAAGEETNC
Sbjct: 241 DPMEGAIEVDWRDFFPYLKWIPNKSVENKIRQMDFCRRVTMMSLIEEQKKRIAAGEETNC 300

Query: 301 YADFLLSEAKTLTEDQISMLLWETIIETSDTTLVDYLYQHIQSVCGSTKLTEDNLSQLPY 360
           YADFLLSEAKTLTE QI+MLLWETIIETSDTTLVDYLYQHI SVCGS KLTED+LS+LPY
Sbjct: 301 YADFLLSEAKTLTEHQIAMLLWETIIETSDTTLVDYLYQHIHSVCGSEKLTEDDLSKLPY 360

Query: 361 LTAVFHETLRKHSPVPIVPLRYAHEDTQLGGFFVPAGSEIAVNIYACNMDKQHWESPEEW 420
           LTAVFHETLRKHSPVPIVPLRY HED QLGGFFVPAGSEIAVNIYACNMDK  WESPEEW
Sbjct: 361 LTAVFHETLRKHSPVPIVPLRYVHEDAQLGGFFVPAGSEIAVNIYACNMDKDRWESPEEW 420

Query: 421 MPERFLDEKSDPMDLHKTMAFGGGKRVCAGALKAMLISCTTIGKLVQEFEWKLREGEEDK 480
            PERFLDEK DPMDLHKTMAFGGGKRVCAGALKAMLI+CT+IG+LVQEFEWKL EGEEDK
Sbjct: 421 KPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKAMLIACTSIGRLVQEFEWKLGEGEEDK 480

Query: 481 VDTLGLTARKLQPLHAIVKPR 502
           VDTLGLTARKLQPLHAIVKPR
Sbjct: 481 VDTLGLTARKLQPLHAIVKPR 500

BLAST of Spg007135 vs. NCBI nr
Match: XP_022976063.1 (ent-kaurene oxidase, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 883.2 bits (2281), Expect = 1.0e-252
Identity = 436/517 (84.33%), Postives = 470/517 (90.91%), Query Frame = 0

Query: 1   MAIVNDPLGSMQHLADALQAPPYAAAAVLGGVSLLVSMFLLGDWRKRRRNLLPPVPAIPG 60
           MAI NDPLGS+QHL D LQAPPYAAAA+LG +S L++ FL+ DWRK RRN LPPVPAIPG
Sbjct: 1   MAIFNDPLGSLQHLLDTLQAPPYAAAAILGVISFLLAGFLVADWRKSRRNFLPPVPAIPG 60

Query: 61  VPVLGNLLQLKEKKPHKTFAQWAETYGPVYSIRTGASTVVVLNTAEVAKEALVTRYSSMS 120
           +PVLGNLLQLKEKKPHKTFA+WAETYGP+YSIRTGASTV+VLNTAEVAKEA+VTRY+SMS
Sbjct: 61  LPVLGNLLQLKEKKPHKTFARWAETYGPIYSIRTGASTVIVLNTAEVAKEAMVTRYASMS 120

Query: 121 SRKLSKALTILTADKCMVAMSDYNDFHKLAKRYILANLLGVNAQKRLRQRRDTMMENICS 180
            RKLSKALTILT+DKCMVAMSDYNDFHK+ K+YILAN+LG NAQKR RQRRD+M++NI S
Sbjct: 121 GRKLSKALTILTSDKCMVAMSDYNDFHKMVKKYILANVLGANAQKRHRQRRDSMIDNISS 180

Query: 181 QLFAQVKDSPSEFVNFRKIFEAELFRLALKETFGKDIESLYVDGLGTTLPREDLFKVLVI 240
           QLFA VKDSPSE VNFRKIFE+ELF LALKET GKDIES+YVDGLGTTLPREDLF+VLVI
Sbjct: 181 QLFAHVKDSPSESVNFRKIFESELFLLALKETLGKDIESIYVDGLGTTLPREDLFRVLVI 240

Query: 241 DPMEGAIEVDWRDFFPYLRWIPNKGLENKIKQMDFRRRMTMKSLIEDQKKRIAAGEETNC 300
           D MEGAIEVDWRDFFPYL+WIPNK +ENKI+QMD+RRR+TM SLIE+QKKRIAAGEETNC
Sbjct: 241 DLMEGAIEVDWRDFFPYLKWIPNKSVENKIRQMDYRRRVTMMSLIEEQKKRIAAGEETNC 300

Query: 301 YADFLLSEAKTLTEDQISMLLWETIIETSDTTLV----------------DYLYQHIQSV 360
           YADFLLSEAKTLTE QI+MLLWETIIETSDTTLV                DYLYQHIQSV
Sbjct: 301 YADFLLSEAKTLTEHQIAMLLWETIIETSDTTLVVTEWAMYELAKNPERQDYLYQHIQSV 360

Query: 361 CGSTKLTEDNLSQLPYLTAVFHETLRKHSPVPIVPLRYAHEDTQLGGFFVPAGSEIAVNI 420
           CGS KLTED+LS+LPYLTAVFHETLRKHSPVPIVPLRY HED QLGGFFVPAGSEIAVNI
Sbjct: 361 CGSEKLTEDDLSKLPYLTAVFHETLRKHSPVPIVPLRYVHEDAQLGGFFVPAGSEIAVNI 420

Query: 421 YACNMDKQHWESPEEWMPERFLDEKSDPMDLHKTMAFGGGKRVCAGALKAMLISCTTIGK 480
           YACNMDK  WESPEEW PERFLDEK DPMDLHKTMAFGGGKRVCAGALKAMLI+CT+IG+
Sbjct: 421 YACNMDKDRWESPEEWKPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKAMLIACTSIGR 480

Query: 481 LVQEFEWKLREGEEDKVDTLGLTARKLQPLHAIVKPR 502
           LVQEFEWKL EGEEDKVDTLGLTARKLQPLH IVKPR
Sbjct: 481 LVQEFEWKLGEGEEDKVDTLGLTARKLQPLHVIVKPR 517

BLAST of Spg007135 vs. NCBI nr
Match: XP_023536271.1 (ent-kaurene oxidase, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 881.3 bits (2276), Expect = 3.9e-252
Identity = 435/517 (84.14%), Postives = 470/517 (90.91%), Query Frame = 0

Query: 1   MAIVNDPLGSMQHLADALQAPPYAAAAVLGGVSLLVSMFLLGDWRKRRRNLLPPVPAIPG 60
           MAI NDPLGS+QHL D LQAP YAAAA+LG +S L++ F + DWRK RRN LPPVPAIPG
Sbjct: 1   MAIFNDPLGSLQHLLDTLQAPTYAAAAILGVISFLLAGFFVADWRKSRRNFLPPVPAIPG 60

Query: 61  VPVLGNLLQLKEKKPHKTFAQWAETYGPVYSIRTGASTVVVLNTAEVAKEALVTRYSSMS 120
           VP+LGNLLQLKEKKPHKTFA+WAE YGP+YSIRTGASTV+VLNTAEVAKEA+VTR++SMS
Sbjct: 61  VPLLGNLLQLKEKKPHKTFARWAERYGPIYSIRTGASTVIVLNTAEVAKEAMVTRHASMS 120

Query: 121 SRKLSKALTILTADKCMVAMSDYNDFHKLAKRYILANLLGVNAQKRLRQRRDTMMENICS 180
           SRKLSKALTILT+DKCMVAMSDYNDFHK+ K+YILAN+LG NAQKR RQRRD+M++NI S
Sbjct: 121 SRKLSKALTILTSDKCMVAMSDYNDFHKMVKKYILANVLGANAQKRHRQRRDSMIDNISS 180

Query: 181 QLFAQVKDSPSEFVNFRKIFEAELFRLALKETFGKDIESLYVDGLGTTLPREDLFKVLVI 240
           QLFA VKDSPSE VNFRKIFE+ELFRLALKET GKDIES+YVDGL TTLPREDLF+VLVI
Sbjct: 181 QLFAHVKDSPSESVNFRKIFESELFRLALKETVGKDIESIYVDGLSTTLPREDLFRVLVI 240

Query: 241 DPMEGAIEVDWRDFFPYLRWIPNKGLENKIKQMDFRRRMTMKSLIEDQKKRIAAGEETNC 300
           DPMEGAIEVDWRDFFPYL+WIPNK +ENKI+QMDFRRR+TM SLIE+QKKRIAAGEETNC
Sbjct: 241 DPMEGAIEVDWRDFFPYLKWIPNKSVENKIRQMDFRRRVTMMSLIEEQKKRIAAGEETNC 300

Query: 301 YADFLLSEAKTLTEDQISMLLWETIIETSDTTLV----------------DYLYQHIQSV 360
           YADFLLSEAKTLTE QI+MLLWETIIETSDTTLV                DYLYQHIQSV
Sbjct: 301 YADFLLSEAKTLTEHQIAMLLWETIIETSDTTLVVTEWAMYELAKNPKRQDYLYQHIQSV 360

Query: 361 CGSTKLTEDNLSQLPYLTAVFHETLRKHSPVPIVPLRYAHEDTQLGGFFVPAGSEIAVNI 420
           CGS KLTED+LS+LPYLTAVFHETLRKHSPVPIVPLRY HED QLGGFFVPAGSEIAVNI
Sbjct: 361 CGSEKLTEDDLSKLPYLTAVFHETLRKHSPVPIVPLRYVHEDAQLGGFFVPAGSEIAVNI 420

Query: 421 YACNMDKQHWESPEEWMPERFLDEKSDPMDLHKTMAFGGGKRVCAGALKAMLISCTTIGK 480
           YACNMDK  WESPEEW PERFLD+K DPMDLHKTMAFGGGKRVCAGALKAMLI+CT+IG+
Sbjct: 421 YACNMDKDRWESPEEWKPERFLDDKYDPMDLHKTMAFGGGKRVCAGALKAMLIACTSIGR 480

Query: 481 LVQEFEWKLREGEEDKVDTLGLTARKLQPLHAIVKPR 502
           LVQEFEWKL EGEEDKVDTLGLTARKLQPLHAIVKPR
Sbjct: 481 LVQEFEWKLGEGEEDKVDTLGLTARKLQPLHAIVKPR 517

BLAST of Spg007135 vs. NCBI nr
Match: XP_008452488.1 (PREDICTED: ent-kaurene oxidase, chloroplastic [Cucumis melo] >KAA0064350.1 ent-kaurene oxidase [Cucumis melo var. makuwa] >TYK20237.1 ent-kaurene oxidase [Cucumis melo var. makuwa])

HSP 1 Score: 880.2 bits (2273), Expect = 8.6e-252
Identity = 430/517 (83.17%), Postives = 472/517 (91.30%), Query Frame = 0

Query: 1   MAIVNDPLGSMQHLADALQAPPYAAAAVLGGVSLLVSMFLLGDWRKRRRNLLPPVPAIPG 60
           MA+V DPL SMQ LA+ L APPYAAAAVLGGVSL++S+FL+ D RK+RRN LPPVPA+PG
Sbjct: 1   MAVVTDPLASMQLLANTLPAPPYAAAAVLGGVSLVLSVFLVADCRKKRRNFLPPVPAVPG 60

Query: 61  VPVLGNLLQLKEKKPHKTFAQWAETYGPVYSIRTGASTVVVLNTAEVAKEALVTRYSSMS 120
           VP+LGNLLQLKEKKPHKTFA+WAETYGPVYSIRTGASTV+VLNT EVAKEA+VTRY S+S
Sbjct: 61  VPLLGNLLQLKEKKPHKTFARWAETYGPVYSIRTGASTVIVLNTTEVAKEAMVTRYGSIS 120

Query: 121 SRKLSKALTILTADKCMVAMSDYNDFHKLAKRYILANLLGVNAQKRLRQRRDTMMENICS 180
           SRKLSKALTILTADKCMVAMSDYN+FHK+ KRYILAN+LG NAQK+ RQRRD M+ENI  
Sbjct: 121 SRKLSKALTILTADKCMVAMSDYNEFHKMVKRYILANVLGANAQKKHRQRRDAMIENISR 180

Query: 181 QLFAQVKDSPSEFVNFRKIFEAELFRLALKETFGKDIESLYVDGLGTTLPREDLFKVLVI 240
           +LFA VK+ PS+ VNFRKIFE+ELFRLALKET GKDIES+YVDGLGTTLPREDLF++LVI
Sbjct: 181 ELFAHVKEFPSDTVNFRKIFESELFRLALKETLGKDIESIYVDGLGTTLPREDLFRILVI 240

Query: 241 DPMEGAIEVDWRDFFPYLRWIPNKGLENKIKQMDFRRRMTMKSLIEDQKKRIAAGEETNC 300
           DPMEGAIEVDWRDFFPYLRWIPNK +ENKI+ MDFRRRMTMKSLI++ K+RIAAGEETNC
Sbjct: 241 DPMEGAIEVDWRDFFPYLRWIPNKSVENKIRNMDFRRRMTMKSLIQEPKRRIAAGEETNC 300

Query: 301 YADFLLSEAKTLTEDQISMLLWETIIETSDTTLV----------------DYLYQHIQSV 360
           YADFLLSEAKTLTEDQISMLLWETIIETSDTTLV                DYLYQ IQSV
Sbjct: 301 YADFLLSEAKTLTEDQISMLLWETIIETSDTTLVVTEWAMYELSKDPKRQDYLYQQIQSV 360

Query: 361 CGSTKLTEDNLSQLPYLTAVFHETLRKHSPVPIVPLRYAHEDTQLGGFFVPAGSEIAVNI 420
           CGS+ LTE+NLSQLPYLTA+FHETLRKHSPVP+VPLRYAHEDTQLGG+FVPAGSEIAVNI
Sbjct: 361 CGSSTLTEENLSQLPYLTAIFHETLRKHSPVPVVPLRYAHEDTQLGGYFVPAGSEIAVNI 420

Query: 421 YACNMDKQHWESPEEWMPERFLDEKSDPMDLHKTMAFGGGKRVCAGALKAMLISCTTIGK 480
           YACNMDK HWE+PEEW PERFLDEK DPMDLHKTMAFGGGKRVCAGALKAMLI+CTTIG+
Sbjct: 421 YACNMDKNHWENPEEWKPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKAMLIACTTIGR 480

Query: 481 LVQEFEWKLREGEEDKVDTLGLTARKLQPLHAIVKPR 502
           +VQEFEWKLREGEE+KVDTLGLTARKLQPLH ++KPR
Sbjct: 481 MVQEFEWKLREGEEEKVDTLGLTARKLQPLHVVIKPR 517

BLAST of Spg007135 vs. ExPASy Swiss-Prot
Match: A0A0G2RKY1 (Ent-kaurene oxidase OS=Salvia miltiorrhiza OX=226208 GN=KO PE=1 SV=1)

HSP 1 Score: 636.3 bits (1640), Expect = 2.8e-181
Identity = 315/507 (62.13%), Postives = 387/507 (76.33%), Query Frame = 0

Query: 17  ALQAPPYAAA-----AVLGGVSLLVSMFLLGDWRKRRRNLLPPVPAIPGVPVLGNLLQLK 76
           +LQA P AAA       +GG++L      + D RK+  +  PP P +PG+PV+GNLLQLK
Sbjct: 6   SLQAVPAAAAIGGPVVAIGGITLFFIREYVKDQRKKSSS-FPPPPKVPGLPVIGNLLQLK 65

Query: 77  EKKPHKTFAQWAETYGPVYSIRTGASTVVVLNTAEVAKEALVTRYSSMSSRKLSKALTIL 136
           EKKPHKTF +W+E YGP+YSIRTGA+T++VLNT +VAKEA++T+YSS+S+RKLSKALTIL
Sbjct: 66  EKKPHKTFTKWSEKYGPIYSIRTGANTMIVLNTNDVAKEAMITKYSSISTRKLSKALTIL 125

Query: 137 TADKCMVAMSDYNDFHKLAKRYILANLLGVNAQKRLRQRRDTMMENICSQLFAQVKDSPS 196
           T+DK +VAMSDYN+F+K AKR++L + LG  AQKR R  R+ M+ NIC Q  A  K  PS
Sbjct: 126 TSDKSIVAMSDYNEFYKAAKRHLLTSTLGPTAQKRHRVHRNLMINNICDQFLAHAKMYPS 185

Query: 197 EFVNFRKIFEAELFRLALKETFGKDIESLYVDGLGTTLPREDLFKVLVIDPMEGAIEVDW 256
           E VNFRKIF++ELF L++K+  G+D+ES+YV+ L TTL R+++FK+LV+DPMEGAI+VDW
Sbjct: 186 EAVNFRKIFQSELFGLSMKQAIGEDVESIYVEDLDTTLSRQEMFKILVVDPMEGAIDVDW 245

Query: 257 RDFFPYLRWIPNKGLENKIKQMDFRRRMTMKSLIEDQKKRIAAGEETNCYADFLLSE-AK 316
           RDFFPYL+WIPN+  ENKI+QM F R   MK+LIE QKKRIA+G+  NCY D LLSE A 
Sbjct: 246 RDFFPYLKWIPNQHFENKIQQMHFHREAVMKALIEQQKKRIASGKAINCYLDHLLSEAAD 305

Query: 317 TLTEDQISMLLWETIIETSDTTLV----------------DYLYQHIQSVCGSTKLTEDN 376
           TL+E QI MLLWE IIE SDTTLV                +YL   IQ+ CG  +L E+ 
Sbjct: 306 TLSEQQILMLLWEAIIEASDTTLVTTEWAMYELSKDPKRQNYLLSEIQNACGFDQLNEEK 365

Query: 377 LSQLPYLTAVFHETLRKHSPVPIVPLRYAHEDTQLGGFFVPAGSEIAVNIYACNMDKQHW 436
           L +LPYL A+F ETLRKHSPVP+VPLRY HE+TQLGG+ +P GSEIA+NIY CNMDK  W
Sbjct: 366 LCRLPYLAAIFQETLRKHSPVPVVPLRYVHEETQLGGYTIPEGSEIAINIYGCNMDKNVW 425

Query: 437 ESPEEWMPERFLDEKSDPMDLHKTMAFGGGKRVCAGALKAMLISCTTIGKLVQEFEWKLR 496
           +SPEEW PERF+  K D  +LHKTMAFGGGKRVCAGAL+AM ISC  IG+LVQE EW+L 
Sbjct: 426 DSPEEWRPERFVFGKDDTTELHKTMAFGGGKRVCAGALQAMTISCIAIGRLVQELEWRLG 485

Query: 497 EGEEDKVDTLGLTARKLQPLHAIVKPR 502
           +GEE  VDTLGLT  KL PL  I+KPR
Sbjct: 486 DGEEANVDTLGLTTHKLHPLQTIIKPR 511

BLAST of Spg007135 vs. ExPASy Swiss-Prot
Match: Q93ZB2 (Ent-kaurene oxidase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=KO PE=1 SV=2)

HSP 1 Score: 603.6 bits (1555), Expect = 2.0e-171
Identity = 293/467 (62.74%), Postives = 366/467 (78.37%), Query Frame = 0

Query: 52  LPPVPAIPGVPVLGNLLQLKEKKPHKTFAQWAETYGPVYSIRTGASTVVVLNTAEVAKEA 111
           LP VP +PG PV+GNLLQLKEKKPHKTF +W+E YGP+YSI+ G+S+++VLN+ E AKEA
Sbjct: 41  LPSVPVVPGFPVIGNLLQLKEKKPHKTFTRWSEIYGPIYSIKMGSSSLIVLNSTETAKEA 100

Query: 112 LVTRYSSMSSRKLSKALTILTADKCMVAMSDYNDFHKLAKRYILANLLGVNAQKRLRQRR 171
           +VTR+SS+S+RKLS ALT+LT DK MVA SDY+DFHKL KR +L  LLG NAQKR R  R
Sbjct: 101 MVTRFSSISTRKLSNALTVLTCDKSMVATSDYDDFHKLVKRCLLNGLLGANAQKRKRHYR 160

Query: 172 DTMMENICSQLFAQVKDSPSEFVNFRKIFEAELFRLALKETFGKDIESLYVDGLGTTLPR 231
           D ++EN+ S+L A  +D P E VNFR IFE ELF +ALK+ FGKD+ES+YV  LG TL +
Sbjct: 161 DALIENVSSKLHAHARDHPQEPVNFRAIFEHELFGVALKQAFGKDVESIYVKELGVTLSK 220

Query: 232 EDLFKVLVIDPMEGAIEVDWRDFFPYLRWIPNKGLENKIKQMDFRRRMTMKSLIEDQKKR 291
           +++FKVLV D MEGAI+VDWRDFFPYL+WIPNK  E +I+Q   RR   M +LI+D+ K+
Sbjct: 221 DEIFKVLVHDMMEGAIDVDWRDFFPYLKWIPNKSFEARIQQKHKRRLAVMNALIQDRLKQ 280

Query: 292 IAAGEETNCYADFLLSEAKTLTEDQISMLLWETIIETSDTTLV----------------D 351
             +  + +CY +FL+SEAKTLT++QI++L+WETIIET+DTTLV                D
Sbjct: 281 NGSESDDDCYLNFLMSEAKTLTKEQIAILVWETIIETADTTLVTTEWAIYELAKHPSVQD 340

Query: 352 YLYQHIQSVCGSTKLTEDNLSQLPYLTAVFHETLRKHSPVPIVPLRYAHEDTQLGGFFVP 411
            L + IQ+VCG  K  E+ LSQ+PYL  VFHETLRK+SP P+VP+RYAHEDTQ+GG+ VP
Sbjct: 341 RLCKEIQNVCGGEKFKEEQLSQVPYLNGVFHETLRKYSPAPLVPIRYAHEDTQIGGYHVP 400

Query: 412 AGSEIAVNIYACNMDKQHWESPEEWMPERFLDE-KSDPMDLHKTMAFGGGKRVCAGALKA 471
           AGSEIA+NIY CNMDK+ WE PE+W PERFLD+ K +  DLHKTMAFG GKRVCAGAL+A
Sbjct: 401 AGSEIAINIYGCNMDKKRWERPEDWWPERFLDDGKYETSDLHKTMAFGAGKRVCAGALQA 460

Query: 472 MLISCTTIGKLVQEFEWKLREGEEDKVDTLGLTARKLQPLHAIVKPR 502
            L++   IG+LVQEFEWKLR+GEE+ VDT GLT++KL PL AI+ PR
Sbjct: 461 SLMAGIAIGRLVQEFEWKLRDGEEENVDTYGLTSQKLYPLMAIINPR 507

BLAST of Spg007135 vs. ExPASy Swiss-Prot
Match: Q5Z5R4 (Ent-kaurene oxidase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP701A6 PE=1 SV=1)

HSP 1 Score: 540.0 bits (1390), Expect = 2.8e-152
Identity = 262/488 (53.69%), Postives = 354/488 (72.54%), Query Frame = 0

Query: 24  AAAAVLGGVSLLVSMFLLGDWRKRRRNLLPPVPAIPGVPVLGNLLQLKEKKPHKTFAQWA 83
           AAAAV G V+        G    R+R   P  PA+PG+P++GNL QLKEKKPH+TFA+WA
Sbjct: 12  AAAAVGGFVAAAALAERAGVIAPRKRPNAP--PAVPGLPIIGNLHQLKEKKPHQTFAKWA 71

Query: 84  ETYGPVYSIRTGASTVVVLNTAEVAKEALVTRYSSMSSRKLSKALTILTADKCMVAMSDY 143
           E YGP+Y+IRTGAS+VVVLN+ EVAKEA+V ++SS+S+RKLSKALT+LT DK MVA SDY
Sbjct: 72  EIYGPIYTIRTGASSVVVLNSTEVAKEAMVAKFSSISTRKLSKALTVLTRDKSMVATSDY 131

Query: 144 NDFHKLAKRYILANLLGVNAQKRLRQRRDTMMENICSQLFAQVKDSPSEFVNFRKIFEAE 203
            DFHK+ KRY+++++LG +AQK+ R  RD M+ N+ S     VKD P   + FR +F+ E
Sbjct: 132 CDFHKMVKRYVMSSMLGTSAQKQFRDIRDMMIHNMLSTFHKLVKDDPHAPLIFRDVFKDE 191

Query: 204 LFRLALKETFGKDIESLYVDGLGTTLPREDLFKVLVIDPMEGAIEVDWRDFFPYLRWIPN 263
           LFRL++ ++ G+D+ S+YVD  G  + +E+++   V D M  AIEVDWRDFFPYL W+PN
Sbjct: 192 LFRLSMIQSLGEDVSSVYVDEFGRDISKEEIYNATVTDMMMCAIEVDWRDFFPYLSWVPN 251

Query: 264 KGLENKIKQMDFRRRMTMKSLIEDQKKRIAAGEETNCYADFLLSEAKTLTEDQISMLLWE 323
           K  E ++   + RR   M++LI+ QK+RI  GE   CY DFLL+E  TLT++Q+ ML+WE
Sbjct: 252 KSFETRVFTTETRRTAVMRALIKQQKERIVRGEAKTCYLDFLLAE-NTLTDEQLMMLVWE 311

Query: 324 TIIETSDTTLV----------------DYLYQHIQSVCGSTKLTEDNLSQLPYLTAVFHE 383
            +IE +DTTLV                + LYQ I+ VCG   +TE++L +LPYL AVFHE
Sbjct: 312 ALIEAADTTLVTTEWAMYELAKNPDKQERLYQEIREVCGDETVTEEHLPRLPYLNAVFHE 371

Query: 384 TLRKHSPVPIVPLRYAHEDTQLGGFFVPAGSEIAVNIYACNMDKQHWESPEEWMPERFLD 443
           TLR+HSPVP++P R+ HEDT+L G+ VPAG+E+ +N+Y CNM+++ WESPEEW+PERF  
Sbjct: 372 TLRRHSPVPLIPPRFVHEDTKLAGYDVPAGTEMVINLYGCNMNRKEWESPEEWVPERFAG 431

Query: 444 EKSDPMDLHKTMAFGGGKRVCAGALKAMLISCTTIGKLVQEFEWKLREGEEDKVDTLGLT 496
            + +  D++KTMAFG G+R CAG+L+A  I+C  + + VQEF W+LREG+E+KVDT+ LT
Sbjct: 432 GRLEVADMYKTMAFGAGRRACAGSLQATHIACAAVARFVQEFGWRLREGDEEKVDTVQLT 491

BLAST of Spg007135 vs. ExPASy Swiss-Prot
Match: Q5Z5R7 (Ent-kaurene oxidase-like 3 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP701A19 PE=2 SV=1)

HSP 1 Score: 533.1 bits (1372), Expect = 3.4e-150
Identity = 261/493 (52.94%), Postives = 351/493 (71.20%), Query Frame = 0

Query: 25  AAAVLGGVSLLVSMFLLGDWRKRRRNLLPPVPAIPGVPVLGNLLQLKEKKPHKTFAQWAE 84
           AAA +GG     ++ +     K RRN   P PA+PG+P++GNL QLKEKKPH+TF +WAE
Sbjct: 15  AAAAVGGFIAAATLAVAPP--KNRRN---PPPAVPGLPIIGNLHQLKEKKPHQTFTKWAE 74

Query: 85  TYGPVYSIRTGASTVVVLNTAEVAKEALVTRYSSMSSRKLSKALTILTADKCMVAMSDYN 144
            YGP+Y+IRTGAS+VVVLN+ EVAKEA+V ++SS+S+RKLSKALT+L+ DK MVA SD  
Sbjct: 75  IYGPIYTIRTGASSVVVLNSTEVAKEAMVAKFSSISTRKLSKALTVLSHDKSMVATSDSG 134

Query: 145 DFHKLAKRYILANLLGVNAQKRLRQRRDTMMENICSQLFAQVKDSPSEFVNFRKIFEAEL 204
           DFHK+ KRYI+ ++LG +AQK+ R  RD ++ N+ S     VKD P   + FR +F+ EL
Sbjct: 135 DFHKMGKRYIMLSMLGTSAQKQFRDTRDMIINNMLSTFHQLVKDDPHAPLIFRDVFKDEL 194

Query: 205 FRLALKETFGKDIESLYVDGLGTTLPREDLFKVLVIDPMEGAIEVDWRDFFPYLRWIPNK 264
           FRL++ ++ G+D+ S+YVD  G  + +E+++   V D M  AIEVDWRDFFPYL W+PNK
Sbjct: 195 FRLSMIQSLGEDVSSVYVDEFGRDISKEEIYNATVTDMMMCAIEVDWRDFFPYLSWVPNK 254

Query: 265 GLENKIKQMDFRRRMTMKSLIEDQKKRIAAGEETNCYADFLLSEAKTLTEDQISMLLWET 324
             E ++   + RR   M++LI+ QK+RI  GE   CY DFLL+E  TLT++Q+ ML+WE 
Sbjct: 255 SFETRVFTTESRRTAVMRALIKQQKERIVRGEARTCYLDFLLAE-NTLTDEQLMMLVWEA 314

Query: 325 IIETSDTTLV----------------DYLYQHIQSVCGSTKLTEDNLSQLPYLTAVFHET 384
           +IE +DTTLV                + LYQ I+ VCG   +TE++L  LPYL AVF ET
Sbjct: 315 LIEAADTTLVTTEWAMYELAKNPDKQERLYQEIREVCGDEAVTEEHLPWLPYLNAVFQET 374

Query: 385 LRKHSPVPIVPLRYAHEDTQLGGFFVPAGSEIAVNIYACNMDKQHWESPEEWMPERFLDE 444
           LR+HSPVP++P R+ +EDT L G+ VPAG+E+ +N+Y CNM+K+ WESPEEW PERF   
Sbjct: 375 LRRHSPVPLIPPRFVNEDTMLAGYDVPAGTEMVINLYGCNMNKKEWESPEEWAPERFAGG 434

Query: 445 KSDPMDLHKTMAFGGGKRVCAGALKAMLISCTTIGKLVQEFEWKLREGEEDKVDTLGLTA 502
           +    D++KTMAFG G+RVCAG+L+A  I+C  I + VQEF W+LREG+E+KVDT+ LTA
Sbjct: 435 RFKVADMYKTMAFGAGRRVCAGSLQATHIACAAIARFVQEFGWRLREGDEEKVDTVQLTA 494

BLAST of Spg007135 vs. ExPASy Swiss-Prot
Match: Q0DBF4 (Ent-sandaracopimaradiene 3-hydroxylase OS=Oryza sativa subsp. japonica OX=39947 GN=CYP701A8 PE=1 SV=1)

HSP 1 Score: 496.5 bits (1277), Expect = 3.5e-139
Identity = 248/500 (49.60%), Postives = 344/500 (68.80%), Query Frame = 0

Query: 24  AAAAVLGGVSLLVSMFLLGD----WRKRRRNLLPPVPAIPGVPVLGNLLQLKEKKPHKTF 83
           A AA +  V  LV+   L D        R+N   P PA+PG+P++GNL QLKEKKPH+TF
Sbjct: 9   AGAAAVAAVGGLVAAAALADKLVAAPPPRKNRANPPPAVPGLPIIGNLHQLKEKKPHQTF 68

Query: 84  AQWAETYGPVYSIRTGASTVVVLNTAEVAKEALVTRYSSMSSRKLSKALTILTADKCMVA 143
           A+W+ETYGP+Y+I+TGAS VVVLN+ EVAKEA++ ++SS+S+RKL KA+++LT  K MVA
Sbjct: 69  AKWSETYGPIYTIKTGASPVVVLNSTEVAKEAMIDKFSSISTRKLPKAMSVLTR-KSMVA 128

Query: 144 MSDYNDFHKLAKRYILANLLGVNAQKRLRQRRDTMMENICSQLFAQVKDSPSEFVNFRKI 203
           +SDY D+ K+AKR I+  +LG NAQK+ R  R+ M+ N+ S L   V   P   +NFR +
Sbjct: 129 ISDYGDYQKMAKRNIMIGMLGFNAQKQFRGTRERMISNVLSTLHKLVSLDPHSPLNFRDV 188

Query: 204 FEAELFRLALKETFGKDIESLYVDGLGTTLPREDLFKVLVIDPMEGAIEVDWRDFFPYLR 263
           +  ELF L+L ++ G+D+ S+YV+  G  + ++++F VLV + M  A+E DWRD+FPYL 
Sbjct: 189 YINELFSLSLIQSLGEDVSSVYVEEFGREISKDEIFDVLVHEMMMCAVEADWRDYFPYLS 248

Query: 264 WIPNKGLENKIKQMDFRRRMTMKSLIEDQKKRIAAGEETNCYADFLLSEAKT--LTEDQI 323
           W+PNK  +  +   +FRR   M +LI+ QK+RIA GE    Y DFLL   ++  LT+DQ+
Sbjct: 249 WLPNKSFDTIVSTTEFRRDAIMNALIKKQKERIARGEARASYIDFLLEAERSAQLTDDQL 308

Query: 324 SMLLWETIIETSDTTLV----------------DYLYQHIQSVCGSTKLTEDNLSQLPYL 383
            +LL E+I+  +DT LV                + LYQ I+  CG   +TED+L +LPYL
Sbjct: 309 MLLLSESILAAADTVLVTTEWTMYEIAKNPDKQELLYQEIREACGGEAVTEDDLPRLPYL 368

Query: 384 TAVFHETLRKHSPVPIVPLRYAHEDTQLGGFFVPAGSEIAVNIYACNMDKQHWESPEEWM 443
            AVFHETLR HSPVP++P R+ H+DT L G+ + AG+++ +N+YAC+MD++ WESP EW 
Sbjct: 369 NAVFHETLRLHSPVPVLPPRFVHDDTTLAGYDIAAGTQMMINVYACHMDEKVWESPGEWS 428

Query: 444 PERFLDEKSDPMDLHKTMAFGGGKRVCAGALKAMLISCTTIGKLVQEFEWKLREGEEDKV 502
           PERFL E  +  D +KTMAFG G+R CAG+L+AM I+C  + +LVQE EW+LREG+ DK 
Sbjct: 429 PERFLGEGFEVADRYKTMAFGAGRRTCAGSLQAMNIACVAVARLVQELEWRLREGDGDKE 488

BLAST of Spg007135 vs. ExPASy TrEMBL
Match: A0A6J1IL06 (ent-kaurene oxidase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111476578 PE=3 SV=1)

HSP 1 Score: 883.2 bits (2281), Expect = 4.9e-253
Identity = 436/517 (84.33%), Postives = 470/517 (90.91%), Query Frame = 0

Query: 1   MAIVNDPLGSMQHLADALQAPPYAAAAVLGGVSLLVSMFLLGDWRKRRRNLLPPVPAIPG 60
           MAI NDPLGS+QHL D LQAPPYAAAA+LG +S L++ FL+ DWRK RRN LPPVPAIPG
Sbjct: 1   MAIFNDPLGSLQHLLDTLQAPPYAAAAILGVISFLLAGFLVADWRKSRRNFLPPVPAIPG 60

Query: 61  VPVLGNLLQLKEKKPHKTFAQWAETYGPVYSIRTGASTVVVLNTAEVAKEALVTRYSSMS 120
           +PVLGNLLQLKEKKPHKTFA+WAETYGP+YSIRTGASTV+VLNTAEVAKEA+VTRY+SMS
Sbjct: 61  LPVLGNLLQLKEKKPHKTFARWAETYGPIYSIRTGASTVIVLNTAEVAKEAMVTRYASMS 120

Query: 121 SRKLSKALTILTADKCMVAMSDYNDFHKLAKRYILANLLGVNAQKRLRQRRDTMMENICS 180
            RKLSKALTILT+DKCMVAMSDYNDFHK+ K+YILAN+LG NAQKR RQRRD+M++NI S
Sbjct: 121 GRKLSKALTILTSDKCMVAMSDYNDFHKMVKKYILANVLGANAQKRHRQRRDSMIDNISS 180

Query: 181 QLFAQVKDSPSEFVNFRKIFEAELFRLALKETFGKDIESLYVDGLGTTLPREDLFKVLVI 240
           QLFA VKDSPSE VNFRKIFE+ELF LALKET GKDIES+YVDGLGTTLPREDLF+VLVI
Sbjct: 181 QLFAHVKDSPSESVNFRKIFESELFLLALKETLGKDIESIYVDGLGTTLPREDLFRVLVI 240

Query: 241 DPMEGAIEVDWRDFFPYLRWIPNKGLENKIKQMDFRRRMTMKSLIEDQKKRIAAGEETNC 300
           D MEGAIEVDWRDFFPYL+WIPNK +ENKI+QMD+RRR+TM SLIE+QKKRIAAGEETNC
Sbjct: 241 DLMEGAIEVDWRDFFPYLKWIPNKSVENKIRQMDYRRRVTMMSLIEEQKKRIAAGEETNC 300

Query: 301 YADFLLSEAKTLTEDQISMLLWETIIETSDTTLV----------------DYLYQHIQSV 360
           YADFLLSEAKTLTE QI+MLLWETIIETSDTTLV                DYLYQHIQSV
Sbjct: 301 YADFLLSEAKTLTEHQIAMLLWETIIETSDTTLVVTEWAMYELAKNPERQDYLYQHIQSV 360

Query: 361 CGSTKLTEDNLSQLPYLTAVFHETLRKHSPVPIVPLRYAHEDTQLGGFFVPAGSEIAVNI 420
           CGS KLTED+LS+LPYLTAVFHETLRKHSPVPIVPLRY HED QLGGFFVPAGSEIAVNI
Sbjct: 361 CGSEKLTEDDLSKLPYLTAVFHETLRKHSPVPIVPLRYVHEDAQLGGFFVPAGSEIAVNI 420

Query: 421 YACNMDKQHWESPEEWMPERFLDEKSDPMDLHKTMAFGGGKRVCAGALKAMLISCTTIGK 480
           YACNMDK  WESPEEW PERFLDEK DPMDLHKTMAFGGGKRVCAGALKAMLI+CT+IG+
Sbjct: 421 YACNMDKDRWESPEEWKPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKAMLIACTSIGR 480

Query: 481 LVQEFEWKLREGEEDKVDTLGLTARKLQPLHAIVKPR 502
           LVQEFEWKL EGEEDKVDTLGLTARKLQPLH IVKPR
Sbjct: 481 LVQEFEWKLGEGEEDKVDTLGLTARKLQPLHVIVKPR 517

BLAST of Spg007135 vs. ExPASy TrEMBL
Match: A0A5D3D9I5 (Ent-kaurene oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G003760 PE=3 SV=1)

HSP 1 Score: 880.2 bits (2273), Expect = 4.2e-252
Identity = 430/517 (83.17%), Postives = 472/517 (91.30%), Query Frame = 0

Query: 1   MAIVNDPLGSMQHLADALQAPPYAAAAVLGGVSLLVSMFLLGDWRKRRRNLLPPVPAIPG 60
           MA+V DPL SMQ LA+ L APPYAAAAVLGGVSL++S+FL+ D RK+RRN LPPVPA+PG
Sbjct: 1   MAVVTDPLASMQLLANTLPAPPYAAAAVLGGVSLVLSVFLVADCRKKRRNFLPPVPAVPG 60

Query: 61  VPVLGNLLQLKEKKPHKTFAQWAETYGPVYSIRTGASTVVVLNTAEVAKEALVTRYSSMS 120
           VP+LGNLLQLKEKKPHKTFA+WAETYGPVYSIRTGASTV+VLNT EVAKEA+VTRY S+S
Sbjct: 61  VPLLGNLLQLKEKKPHKTFARWAETYGPVYSIRTGASTVIVLNTTEVAKEAMVTRYGSIS 120

Query: 121 SRKLSKALTILTADKCMVAMSDYNDFHKLAKRYILANLLGVNAQKRLRQRRDTMMENICS 180
           SRKLSKALTILTADKCMVAMSDYN+FHK+ KRYILAN+LG NAQK+ RQRRD M+ENI  
Sbjct: 121 SRKLSKALTILTADKCMVAMSDYNEFHKMVKRYILANVLGANAQKKHRQRRDAMIENISR 180

Query: 181 QLFAQVKDSPSEFVNFRKIFEAELFRLALKETFGKDIESLYVDGLGTTLPREDLFKVLVI 240
           +LFA VK+ PS+ VNFRKIFE+ELFRLALKET GKDIES+YVDGLGTTLPREDLF++LVI
Sbjct: 181 ELFAHVKEFPSDTVNFRKIFESELFRLALKETLGKDIESIYVDGLGTTLPREDLFRILVI 240

Query: 241 DPMEGAIEVDWRDFFPYLRWIPNKGLENKIKQMDFRRRMTMKSLIEDQKKRIAAGEETNC 300
           DPMEGAIEVDWRDFFPYLRWIPNK +ENKI+ MDFRRRMTMKSLI++ K+RIAAGEETNC
Sbjct: 241 DPMEGAIEVDWRDFFPYLRWIPNKSVENKIRNMDFRRRMTMKSLIQEPKRRIAAGEETNC 300

Query: 301 YADFLLSEAKTLTEDQISMLLWETIIETSDTTLV----------------DYLYQHIQSV 360
           YADFLLSEAKTLTEDQISMLLWETIIETSDTTLV                DYLYQ IQSV
Sbjct: 301 YADFLLSEAKTLTEDQISMLLWETIIETSDTTLVVTEWAMYELSKDPKRQDYLYQQIQSV 360

Query: 361 CGSTKLTEDNLSQLPYLTAVFHETLRKHSPVPIVPLRYAHEDTQLGGFFVPAGSEIAVNI 420
           CGS+ LTE+NLSQLPYLTA+FHETLRKHSPVP+VPLRYAHEDTQLGG+FVPAGSEIAVNI
Sbjct: 361 CGSSTLTEENLSQLPYLTAIFHETLRKHSPVPVVPLRYAHEDTQLGGYFVPAGSEIAVNI 420

Query: 421 YACNMDKQHWESPEEWMPERFLDEKSDPMDLHKTMAFGGGKRVCAGALKAMLISCTTIGK 480
           YACNMDK HWE+PEEW PERFLDEK DPMDLHKTMAFGGGKRVCAGALKAMLI+CTTIG+
Sbjct: 421 YACNMDKNHWENPEEWKPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKAMLIACTTIGR 480

Query: 481 LVQEFEWKLREGEEDKVDTLGLTARKLQPLHAIVKPR 502
           +VQEFEWKLREGEE+KVDTLGLTARKLQPLH ++KPR
Sbjct: 481 MVQEFEWKLREGEEEKVDTLGLTARKLQPLHVVIKPR 517

BLAST of Spg007135 vs. ExPASy TrEMBL
Match: A0A1S3BTZ0 (ent-kaurene oxidase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103493505 PE=3 SV=1)

HSP 1 Score: 880.2 bits (2273), Expect = 4.2e-252
Identity = 430/517 (83.17%), Postives = 472/517 (91.30%), Query Frame = 0

Query: 1   MAIVNDPLGSMQHLADALQAPPYAAAAVLGGVSLLVSMFLLGDWRKRRRNLLPPVPAIPG 60
           MA+V DPL SMQ LA+ L APPYAAAAVLGGVSL++S+FL+ D RK+RRN LPPVPA+PG
Sbjct: 1   MAVVTDPLASMQLLANTLPAPPYAAAAVLGGVSLVLSVFLVADCRKKRRNFLPPVPAVPG 60

Query: 61  VPVLGNLLQLKEKKPHKTFAQWAETYGPVYSIRTGASTVVVLNTAEVAKEALVTRYSSMS 120
           VP+LGNLLQLKEKKPHKTFA+WAETYGPVYSIRTGASTV+VLNT EVAKEA+VTRY S+S
Sbjct: 61  VPLLGNLLQLKEKKPHKTFARWAETYGPVYSIRTGASTVIVLNTTEVAKEAMVTRYGSIS 120

Query: 121 SRKLSKALTILTADKCMVAMSDYNDFHKLAKRYILANLLGVNAQKRLRQRRDTMMENICS 180
           SRKLSKALTILTADKCMVAMSDYN+FHK+ KRYILAN+LG NAQK+ RQRRD M+ENI  
Sbjct: 121 SRKLSKALTILTADKCMVAMSDYNEFHKMVKRYILANVLGANAQKKHRQRRDAMIENISR 180

Query: 181 QLFAQVKDSPSEFVNFRKIFEAELFRLALKETFGKDIESLYVDGLGTTLPREDLFKVLVI 240
           +LFA VK+ PS+ VNFRKIFE+ELFRLALKET GKDIES+YVDGLGTTLPREDLF++LVI
Sbjct: 181 ELFAHVKEFPSDTVNFRKIFESELFRLALKETLGKDIESIYVDGLGTTLPREDLFRILVI 240

Query: 241 DPMEGAIEVDWRDFFPYLRWIPNKGLENKIKQMDFRRRMTMKSLIEDQKKRIAAGEETNC 300
           DPMEGAIEVDWRDFFPYLRWIPNK +ENKI+ MDFRRRMTMKSLI++ K+RIAAGEETNC
Sbjct: 241 DPMEGAIEVDWRDFFPYLRWIPNKSVENKIRNMDFRRRMTMKSLIQEPKRRIAAGEETNC 300

Query: 301 YADFLLSEAKTLTEDQISMLLWETIIETSDTTLV----------------DYLYQHIQSV 360
           YADFLLSEAKTLTEDQISMLLWETIIETSDTTLV                DYLYQ IQSV
Sbjct: 301 YADFLLSEAKTLTEDQISMLLWETIIETSDTTLVVTEWAMYELSKDPKRQDYLYQQIQSV 360

Query: 361 CGSTKLTEDNLSQLPYLTAVFHETLRKHSPVPIVPLRYAHEDTQLGGFFVPAGSEIAVNI 420
           CGS+ LTE+NLSQLPYLTA+FHETLRKHSPVP+VPLRYAHEDTQLGG+FVPAGSEIAVNI
Sbjct: 361 CGSSTLTEENLSQLPYLTAIFHETLRKHSPVPVVPLRYAHEDTQLGGYFVPAGSEIAVNI 420

Query: 421 YACNMDKQHWESPEEWMPERFLDEKSDPMDLHKTMAFGGGKRVCAGALKAMLISCTTIGK 480
           YACNMDK HWE+PEEW PERFLDEK DPMDLHKTMAFGGGKRVCAGALKAMLI+CTTIG+
Sbjct: 421 YACNMDKNHWENPEEWKPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKAMLIACTTIGR 480

Query: 481 LVQEFEWKLREGEEDKVDTLGLTARKLQPLHAIVKPR 502
           +VQEFEWKLREGEE+KVDTLGLTARKLQPLH ++KPR
Sbjct: 481 MVQEFEWKLREGEEEKVDTLGLTARKLQPLHVVIKPR 517

BLAST of Spg007135 vs. ExPASy TrEMBL
Match: A0A6J1F7C8 (ent-kaurene oxidase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111442772 PE=3 SV=1)

HSP 1 Score: 878.6 bits (2269), Expect = 1.2e-251
Identity = 436/517 (84.33%), Postives = 469/517 (90.72%), Query Frame = 0

Query: 1   MAIVNDPLGSMQHLADALQAPPYAAAAVLGGVSLLVSMFLLGDWRKRRRNLLPPVPAIPG 60
           M I NDPLGS+QHL D LQAP YAAA++LG +S L++ FL+ DWRK RRN LPPVPAIPG
Sbjct: 1   MVIFNDPLGSLQHLLDTLQAPTYAAASILGVISFLLAGFLVADWRKSRRNFLPPVPAIPG 60

Query: 61  VPVLGNLLQLKEKKPHKTFAQWAETYGPVYSIRTGASTVVVLNTAEVAKEALVTRYSSMS 120
           +PVLGNLLQLKEKKPHKTFA+WAETYGP+YSIRTGASTV+VLNTAEVAKEA+VTRY+SMS
Sbjct: 61  LPVLGNLLQLKEKKPHKTFARWAETYGPIYSIRTGASTVIVLNTAEVAKEAMVTRYASMS 120

Query: 121 SRKLSKALTILTADKCMVAMSDYNDFHKLAKRYILANLLGVNAQKRLRQRRDTMMENICS 180
           SRKLSKALTILT+DKCMVAMSDYNDFHK+ K+YILAN+LG NAQKR RQRRDTM++NI S
Sbjct: 121 SRKLSKALTILTSDKCMVAMSDYNDFHKMVKKYILANVLGANAQKRHRQRRDTMIDNISS 180

Query: 181 QLFAQVKDSPSEFVNFRKIFEAELFRLALKETFGKDIESLYVDGLGTTLPREDLFKVLVI 240
           QLFA VKDSPSE VNFR+IFE+ELFRLALKET GKDIES+YVDGLG  LPREDLF+VLVI
Sbjct: 181 QLFAHVKDSPSESVNFRRIFESELFRLALKETLGKDIESIYVDGLG-MLPREDLFRVLVI 240

Query: 241 DPMEGAIEVDWRDFFPYLRWIPNKGLENKIKQMDFRRRMTMKSLIEDQKKRIAAGEETNC 300
           DPMEGAIEVDWRDFFPYL+WIPNK +ENKI+QMDFRRR+TM SLIE QKKRIAAGEETNC
Sbjct: 241 DPMEGAIEVDWRDFFPYLKWIPNKSVENKIRQMDFRRRVTMMSLIEKQKKRIAAGEETNC 300

Query: 301 YADFLLSEAKTLTEDQISMLLWETIIETSDTTLV----------------DYLYQHIQSV 360
           YADFLLSEAKTLTE QI+MLLWETIIETSDTTLV                DYLYQHIQSV
Sbjct: 301 YADFLLSEAKTLTEHQIAMLLWETIIETSDTTLVVTEWAMYELAKNPKRQDYLYQHIQSV 360

Query: 361 CGSTKLTEDNLSQLPYLTAVFHETLRKHSPVPIVPLRYAHEDTQLGGFFVPAGSEIAVNI 420
           CGS KLTED+LS+LPYLTAVFHETLRKHSPVPIVPLRY HED QLGGFFVPAGSEIAVNI
Sbjct: 361 CGSEKLTEDDLSKLPYLTAVFHETLRKHSPVPIVPLRYVHEDAQLGGFFVPAGSEIAVNI 420

Query: 421 YACNMDKQHWESPEEWMPERFLDEKSDPMDLHKTMAFGGGKRVCAGALKAMLISCTTIGK 480
           YACNMDK  WESPEEW PERFLDEK DPMDLHKTMAFGGGKRVCAGALKAMLI+CT+IG+
Sbjct: 421 YACNMDKDRWESPEEWKPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKAMLIACTSIGR 480

Query: 481 LVQEFEWKLREGEEDKVDTLGLTARKLQPLHAIVKPR 502
           LVQEFEWKL EGEEDKVDTLGLTARKLQPLHAIVKPR
Sbjct: 481 LVQEFEWKLGEGEEDKVDTLGLTARKLQPLHAIVKPR 516

BLAST of Spg007135 vs. ExPASy TrEMBL
Match: J7I3T1 (Ent-kaurene oxidase OS=Cucumis sativus OX=3659 GN=ko PE=2 SV=1)

HSP 1 Score: 870.2 bits (2247), Expect = 4.3e-249
Identity = 427/517 (82.59%), Postives = 466/517 (90.14%), Query Frame = 0

Query: 1   MAIVNDPLGSMQHLADALQAPPYAAAAVLGGVSLLVSMFLLGDWRKRRRNLLPPVPAIPG 60
           MA+V DPL SMQ LA+ + APPYAAAAVLGGVSL++S+F + D RK+RRN LPPVPA+PG
Sbjct: 1   MAVVTDPLASMQLLANTIPAPPYAAAAVLGGVSLVLSVFFVADCRKKRRNFLPPVPAVPG 60

Query: 61  VPVLGNLLQLKEKKPHKTFAQWAETYGPVYSIRTGASTVVVLNTAEVAKEALVTRYSSMS 120
           VPVLGNLLQLKEKKPHKTFA+WAETYG VYSIRTGASTV+VLNT EVAKEA+VTRY S+S
Sbjct: 61  VPVLGNLLQLKEKKPHKTFARWAETYGAVYSIRTGASTVIVLNTTEVAKEAMVTRYGSIS 120

Query: 121 SRKLSKALTILTADKCMVAMSDYNDFHKLAKRYILANLLGVNAQKRLRQRRDTMMENICS 180
           SRKLSKALTILTADKCMVAMSDYN+FHK+ KRYILAN+LG NAQK+ RQRRD M+ENI  
Sbjct: 121 SRKLSKALTILTADKCMVAMSDYNEFHKMVKRYILANVLGANAQKKHRQRRDAMIENISR 180

Query: 181 QLFAQVKDSPSEFVNFRKIFEAELFRLALKETFGKDIESLYVDGLGTTLPREDLFKVLVI 240
           +LFA VK+ P + VNFRKIFEAELFRLALKET GKDIES+YVDGLGTTLPREDLF++LVI
Sbjct: 181 ELFAHVKEFPLDTVNFRKIFEAELFRLALKETLGKDIESIYVDGLGTTLPREDLFRILVI 240

Query: 241 DPMEGAIEVDWRDFFPYLRWIPNKGLENKIKQMDFRRRMTMKSLIEDQKKRIAAGEETNC 300
           DPMEGAIEVDWRDFFPYLRWIPNK +ENKI+ MDFRRRMTMK L+E+ KKRIAAGEET C
Sbjct: 241 DPMEGAIEVDWRDFFPYLRWIPNKRVENKIRNMDFRRRMTMKKLMEEPKKRIAAGEETYC 300

Query: 301 YADFLLSEAKTLTEDQISMLLWETIIETSDTTLV----------------DYLYQHIQSV 360
           YADFLLSEAKTLTEDQISMLLWETIIETSDTTLV                DYLYQ IQSV
Sbjct: 301 YADFLLSEAKTLTEDQISMLLWETIIETSDTTLVVTEWAMYELSKDPRRQDYLYQQIQSV 360

Query: 361 CGSTKLTEDNLSQLPYLTAVFHETLRKHSPVPIVPLRYAHEDTQLGGFFVPAGSEIAVNI 420
           CGS  LTE+NLSQLPYLTA+FHETLRKHSPVP+VPLRYAHEDTQLGG+FVPAGSEIAVNI
Sbjct: 361 CGSATLTEENLSQLPYLTAIFHETLRKHSPVPVVPLRYAHEDTQLGGYFVPAGSEIAVNI 420

Query: 421 YACNMDKQHWESPEEWMPERFLDEKSDPMDLHKTMAFGGGKRVCAGALKAMLISCTTIGK 480
           YACNMDK HWESPEEW PERFLD+K DPMDLHKTMAFGGGKRVCAGALKAMLI+CTTIG+
Sbjct: 421 YACNMDKDHWESPEEWKPERFLDDKYDPMDLHKTMAFGGGKRVCAGALKAMLIACTTIGR 480

Query: 481 LVQEFEWKLREGEEDKVDTLGLTARKLQPLHAIVKPR 502
           +VQEFEWKLREGEE+KVDTLGLTARKLQPLH ++KPR
Sbjct: 481 MVQEFEWKLREGEEEKVDTLGLTARKLQPLHVVIKPR 517

BLAST of Spg007135 vs. TAIR 10
Match: AT5G25900.1 (GA requiring 3 )

HSP 1 Score: 603.6 bits (1555), Expect = 1.5e-172
Identity = 293/467 (62.74%), Postives = 366/467 (78.37%), Query Frame = 0

Query: 52  LPPVPAIPGVPVLGNLLQLKEKKPHKTFAQWAETYGPVYSIRTGASTVVVLNTAEVAKEA 111
           LP VP +PG PV+GNLLQLKEKKPHKTF +W+E YGP+YSI+ G+S+++VLN+ E AKEA
Sbjct: 41  LPSVPVVPGFPVIGNLLQLKEKKPHKTFTRWSEIYGPIYSIKMGSSSLIVLNSTETAKEA 100

Query: 112 LVTRYSSMSSRKLSKALTILTADKCMVAMSDYNDFHKLAKRYILANLLGVNAQKRLRQRR 171
           +VTR+SS+S+RKLS ALT+LT DK MVA SDY+DFHKL KR +L  LLG NAQKR R  R
Sbjct: 101 MVTRFSSISTRKLSNALTVLTCDKSMVATSDYDDFHKLVKRCLLNGLLGANAQKRKRHYR 160

Query: 172 DTMMENICSQLFAQVKDSPSEFVNFRKIFEAELFRLALKETFGKDIESLYVDGLGTTLPR 231
           D ++EN+ S+L A  +D P E VNFR IFE ELF +ALK+ FGKD+ES+YV  LG TL +
Sbjct: 161 DALIENVSSKLHAHARDHPQEPVNFRAIFEHELFGVALKQAFGKDVESIYVKELGVTLSK 220

Query: 232 EDLFKVLVIDPMEGAIEVDWRDFFPYLRWIPNKGLENKIKQMDFRRRMTMKSLIEDQKKR 291
           +++FKVLV D MEGAI+VDWRDFFPYL+WIPNK  E +I+Q   RR   M +LI+D+ K+
Sbjct: 221 DEIFKVLVHDMMEGAIDVDWRDFFPYLKWIPNKSFEARIQQKHKRRLAVMNALIQDRLKQ 280

Query: 292 IAAGEETNCYADFLLSEAKTLTEDQISMLLWETIIETSDTTLV----------------D 351
             +  + +CY +FL+SEAKTLT++QI++L+WETIIET+DTTLV                D
Sbjct: 281 NGSESDDDCYLNFLMSEAKTLTKEQIAILVWETIIETADTTLVTTEWAIYELAKHPSVQD 340

Query: 352 YLYQHIQSVCGSTKLTEDNLSQLPYLTAVFHETLRKHSPVPIVPLRYAHEDTQLGGFFVP 411
            L + IQ+VCG  K  E+ LSQ+PYL  VFHETLRK+SP P+VP+RYAHEDTQ+GG+ VP
Sbjct: 341 RLCKEIQNVCGGEKFKEEQLSQVPYLNGVFHETLRKYSPAPLVPIRYAHEDTQIGGYHVP 400

Query: 412 AGSEIAVNIYACNMDKQHWESPEEWMPERFLDE-KSDPMDLHKTMAFGGGKRVCAGALKA 471
           AGSEIA+NIY CNMDK+ WE PE+W PERFLD+ K +  DLHKTMAFG GKRVCAGAL+A
Sbjct: 401 AGSEIAINIYGCNMDKKRWERPEDWWPERFLDDGKYETSDLHKTMAFGAGKRVCAGALQA 460

Query: 472 MLISCTTIGKLVQEFEWKLREGEEDKVDTLGLTARKLQPLHAIVKPR 502
            L++   IG+LVQEFEWKLR+GEE+ VDT GLT++KL PL AI+ PR
Sbjct: 461 SLMAGIAIGRLVQEFEWKLRDGEEENVDTYGLTSQKLYPLMAIINPR 507

BLAST of Spg007135 vs. TAIR 10
Match: AT2G40890.1 (cytochrome P450, family 98, subfamily A, polypeptide 3 )

HSP 1 Score: 161.0 bits (406), Expect = 2.5e-39
Identity = 123/484 (25.41%), Postives = 226/484 (46.69%), Query Frame = 0

Query: 46  KRRRNLLPPVPAIPGVPVLGNLLQLKEKKPHKTFAQWAETYGPVYSIRTGASTVVVLNTA 105
           +R R   PP P+    P++GNL  +K  +  + + +WA++YGP+ S+  G+   VV+++A
Sbjct: 21  QRLRYKFPPGPS--PKPIVGNLYDIKPVR-FRCYYEWAQSYGPIISVWIGSILNVVVSSA 80

Query: 106 EVAKEALVTRYSSMSSRKLSKALTILTADKCMVAMSDYNDFHKLAKRYILANLLGVNAQK 165
           E+AKE L      ++ R  +++    + +   +  +DY   +   ++     L      +
Sbjct: 81  ELAKEVLKEHDQKLADRHRNRSTEAFSRNGQDLIWADYGPHYVKVRKVCTLELFTPKRLE 140

Query: 166 RLRQRRDTMMENICSQLF--AQVKDSPSEFVNFRKIFEAELFRLALKETFGKDIESLYVD 225
            LR  R+  +  +   +F    + ++ ++ +  RK   A  F    +  FGK     +++
Sbjct: 141 SLRPIREDEVTAMVESVFRDCNLPENRAKGLQLRKYLGAVAFNNITRLAFGK----RFMN 200

Query: 226 GLGTTLPREDLFKVLVIDPMEGAIEVDWRDFFPYLRWIPNKGLENKIKQMDFRRRMTMKS 285
             G    +   FK +V + ++    +   +  P+LRW+     E    +   RR    ++
Sbjct: 201 AEGVVDEQGLEFKAIVSNGLKLGASLSIAEHIPWLRWM-FPADEKAFAEHGARRDRLTRA 260

Query: 286 LIEDQ---KKRIAAGEETNCYADFLLSEAKTLTEDQISMLLWETIIETSDTTLV------ 345
           ++E+    +++ +  ++    A   L +   L+ED I  LLW+ I    DTT +      
Sbjct: 261 IMEEHTLARQKSSGAKQHFVDALLTLKDQYDLSEDTIIGLLWDMITAGMDTTAITAEWAM 320

Query: 346 ----------DYLYQHIQSVCGSTK-LTEDNLSQLPYLTAVFHETLRKHSPVPIVPLRYA 405
                       + +    V G  + LTE + S+LPYL  V  E+ R H P P++    +
Sbjct: 321 AEMIKNPRVQQKVQEEFDRVVGLDRILTEADFSRLPYLQCVVKESFRLHPPTPLMLPHRS 380

Query: 406 HEDTQLGGFFVPAGSEIAVNIYACNMDKQHWESPEEWMPERFLDEKSDPMDLH--KTMAF 465
           + D ++GG+ +P GS + VN++A   D   W++P E+ PERFL+E  D M  H  + + F
Sbjct: 381 NADVKIGGYDIPKGSNVHVNVWAVARDPAVWKNPFEFRPERFLEEDVD-MKGHDFRLLPF 440

Query: 466 GGGKRVCAGALKAMLISCTTIGKLVQEFEWKLREG----EEDKVDTLGLTARKLQPLHAI 502
           G G+RVC GA   + +  + +  L+  F W   +G    E D  +  GL      P+ A+
Sbjct: 441 GAGRRVCPGAQLGINLVTSMMSHLLHHFVWTPPQGTKPEEIDMSENPGLVTYMRTPVQAV 495

BLAST of Spg007135 vs. TAIR 10
Match: AT4G13770.1 (cytochrome P450, family 83, subfamily A, polypeptide 1 )

HSP 1 Score: 160.6 bits (405), Expect = 3.3e-39
Identity = 128/503 (25.45%), Postives = 240/503 (47.71%), Query Frame = 0

Query: 28  VLGGVSL-LVSMFLLGDWRKRRRNLLPPVPAIPGVPVLGNLLQLKEKKPHKTFAQWAETY 87
           ++G V+L  V +F L    K +R  LPP P+   +PV+GNLLQL++  P + FA WA+ Y
Sbjct: 5   IIGVVALAAVLLFFLYQKPKTKRYKLPPGPS--PLPVIGNLLQLQKLNPQRFFAGWAKKY 64

Query: 88  GPVYSIRTGASTVVVLNTAEVAKEALVTRYSSMSSRKLSKALTILTADKCMVAMSDYNDF 147
           GP+ S R G+ T+VV+++AE+AKE L T+  + + R   +    ++  +  +A++ Y  +
Sbjct: 65  GPILSYRIGSRTMVVISSAELAKELLKTQDVNFADRPPHRGHEFISYGRRDMALNHYTPY 124

Query: 148 HKLAKRYILANLLGVNAQKRLRQRRDTMMENICSQLFAQVKDSPSEFVNFRKIFEAELFR 207
           ++  ++  + +L         +  R+     +  ++        SE V+  ++       
Sbjct: 125 YREIRKMGMNHLFSPTRVATFKHVREEEARRMMDKI--NKAADKSEVVDISELMLTFTNS 184

Query: 208 LALKETFGKDIESLYVDGLGTTLPREDLFKVLVIDPMEGAIEVDWRDFFPYLRWIPN-KG 267
           +  ++ FGK       DG          F  ++        ++ + DFFPY  ++ +  G
Sbjct: 185 VVCRQAFGKKYNE---DG-----EEMKRFIKILYGTQSVLGKIFFSDFFPYCGFLDDLSG 244

Query: 268 LENKIKQMDFRRRMTMKSLIEDQKKRIAAGEETNCYADFLLS------EAKTLTEDQISM 327
           L   +K+   R+   ++ ++ +         ET    D L+        A   T D +  
Sbjct: 245 LTAYMKECFERQDTYIQEVVNETLDPKRVKPETESMIDLLMGIYKEQPFASEFTVDNVKA 304

Query: 328 LLWETIIETSDTTL------VDYLYQHIQSV-------------CGSTKLTEDNLSQLPY 387
           ++ + ++  +DT        + YL ++ Q +              GST +TED++  LPY
Sbjct: 305 VILDIVVAGTDTAAAAVVWGMTYLMKYPQVLKKAQAEVREYMKEKGSTFVTEDDVKNLPY 364

Query: 388 LTAVFHETLRKHSPVPIVPLRYAHEDTQLGGFFVPAGSEIAVNIYACNMDKQHW-ESPEE 447
             A+  ETLR    +P++  R   +DT++ G+ +PAG+ + VN +A + D++ W  +P+E
Sbjct: 365 FRALVKETLRIEPVIPLLIPRACIQDTKIAGYDIPAGTTVNVNAWAVSRDEKEWGPNPDE 424

Query: 448 WMPERFLDEKSDPMDL-HKTMAFGGGKRVCAGALKAMLISCTTIGKLVQEFEWKLREG-- 498
           + PERFL+++ D     ++ + FG G+R+C G      +       L+  F +KL  G  
Sbjct: 425 FRPERFLEKEVDFKGTDYEFIPFGSGRRMCPGMRLGAAMLEVPYANLLLSFNFKLPNGMK 484

BLAST of Spg007135 vs. TAIR 10
Match: AT1G74550.1 (cytochrome P450, family 98, subfamily A, polypeptide 9 )

HSP 1 Score: 156.8 bits (395), Expect = 4.7e-38
Identity = 123/480 (25.62%), Postives = 222/480 (46.25%), Query Frame = 0

Query: 46  KRRRNLLPPVPAIPGVPVLGNLLQLKEKKPHKTFAQWAETYGPVYSIRTGASTVVVLNTA 105
           +RR + +PP P  P   ++GNL QLK     ++F++W++TYGP+ S+  G+   VV++++
Sbjct: 21  RRRGSNIPPGP--PTRFLVGNLHQLKPLWT-QSFSEWSQTYGPIISVWLGSQLAVVVSSS 80

Query: 106 EVAKEALVTRYSSMSSRKLSKALTILTADKCMVAMSDYNDFHKLAKRYILANLLGVNAQK 165
           ++AK+ L  +   + +R  +  +T   +D   +  SDY   +   ++     L  + + +
Sbjct: 81  DLAKQVLRDKDYQLCNRHRTARMTQNGSD---LIWSDYGAHYVKMRKLCTLELFSLKSIE 140

Query: 166 RLRQRRDTMMENICSQLFAQVKDSPSEFVNFRKIFEAELFRLALKETFGKDIESLYVDGL 225
             R  R+  + ++   +F        + V  R   ++    +  +   GK  E       
Sbjct: 141 CFRSMREMEVSSMVKSIFNDFMSDDQKPVVLRNYLDSVALNIVSRLVIGKTFEPKDGREF 200

Query: 226 GTTLPREDLFKVLVIDPMEGAIEVDWRDFFPYLRWIPNKGLENK--IKQMDFRRRMTMKS 285
            + + RE          + GA ++   D+  +L+ + +    +K  +K M  R+R   K 
Sbjct: 201 RSIVERE--------TRLPGATKM--LDYTVWLKRLSSWFTSDKAFMKHM-ARKRNWFKR 260

Query: 286 LIEDQKKRIAAGEETNCYADFL--LSEAKTLTEDQISMLLWETIIETSDTTLV------- 345
            + D+   +  G +  C+   L  L E   LTE+ +  L+W  +   +DTT +       
Sbjct: 261 AVMDE---VYGGRDQKCFVQSLLELKEKDELTEETVMGLVWNMLTAGADTTAITIEWAMA 320

Query: 346 ---------DYLYQHIQSVCGSTKLTED-NLSQLPYLTAVFHETLRKHSPVPIVPLRYAH 405
                    + +   + SV GS +L  D ++ +LP+L  V  E LR H P P++    A 
Sbjct: 321 EMIRCPTVKEKVQDELDSVVGSGRLMSDADIPKLPFLQCVLKEALRLHPPTPLMLPHKAS 380

Query: 406 EDTQLGGFFVPAGSEIAVNIYACNMDKQHWESPEEWMPERFLDEKSDPMDL-HKTMAFGG 465
           E  Q+GG+ VP G+ + VN+ A   D  +W +P+E+ PERFL E++D      + + FG 
Sbjct: 381 ESVQVGGYKVPKGATVYVNVQAIARDPANWSNPDEFRPERFLVEETDVKGQDFRVLPFGS 440

Query: 466 GKRVCAGALKAMLISCTTIGKLVQEFEWKLREGEE--DKVDTLGLTARKLQPLHAIVKPR 502
           G+RVC  A  ++ +    +G L+  F W      E  D  +  GL      PL A+   R
Sbjct: 441 GRRVCPAAQLSLNMMTLALGSLLHCFSWTSSTPREHIDMTEKPGLVCYMKAPLQALASSR 480

BLAST of Spg007135 vs. TAIR 10
Match: AT2G45570.1 (cytochrome P450, family 76, subfamily C, polypeptide 2 )

HSP 1 Score: 156.4 bits (394), Expect = 6.1e-38
Identity = 132/488 (27.05%), Postives = 217/488 (44.47%), Query Frame = 0

Query: 49  RNLLPPVPAIPGVPVLGNLLQLKEKKPHKTFAQWAETYGPVYSIRTGASTVVVLNTAEVA 108
           R ++P  P  P +P++GN + L  + PH +FA  ++TYGP+ S++ G+   VV+ + E A
Sbjct: 33  RKVVPSPPGPPRLPIIGN-IHLVGRNPHHSFADLSKTYGPIMSLKFGSLNTVVVTSPEAA 92

Query: 109 KEALVTRYSSMSSRKLSKALTILTADKCMVA-MSDYNDFHKLAKRYILANLLGVNAQKRL 168
           +E L T    +SSR  + ++  +  DK  V  +   +   +L ++     L      +  
Sbjct: 93  REVLRTYDQILSSRTPTNSIRSINHDKVSVVWLPPSSSRWRLLRKLSATQLFSPQRIEAT 152

Query: 169 RQRRDTMMENICSQLFAQVKDSPSEFVNFRKIFEAELFRLALKETFGKDIESLYVDGLGT 228
           +  R+  ++ + S  F        E V+  +        +     F  D+ +   +  G 
Sbjct: 153 KTLRENKVKELVS--FMSESSEREEAVDISRATFITALNIISNILFSVDLGNYDSNKSG- 212

Query: 229 TLPREDLFKVLVIDPMEGAIEVDWRDFFPYLRWIPNKGLENKIKQMDFRRRMTMKSLIED 288
                 +F+  VI  ME     D  +FFP+L ++  +G    +K    R     +  I D
Sbjct: 213 ------VFQDTVIGVMEAVGNPDAANFFPFLGFLDLQGNRKTLKACSERLFKVFRGFI-D 272

Query: 289 QKKRIAAGEETNC-------YADFLL-----SEAKTLTEDQISMLL------WETIIETS 348
            K    +  +TN        + D LL      EA+  T D + +LL       +T   T 
Sbjct: 273 AKLAEKSLRDTNSKDVRERDFVDVLLDLTEGDEAELNTNDIVHLLLDLFGAGTDTNSSTV 332

Query: 349 DTTLVDYL---------YQHIQSVCGSTKLTED-NLSQLPYLTAVFHETLRKHSPVPIVP 408
           +  + + L            I  V G   + E+ ++S LPYL AV  ET R H   P++ 
Sbjct: 333 EWAMAELLRNPETMVKAQAEIDCVIGQKGVVEESDISALPYLQAVVKETFRLHPAAPLLV 392

Query: 409 LRYAHEDTQLGGFFVPAGSEIAVNIYACNMDKQHWESPEEWMPERFLDEKSDPMDL-HKT 468
            R A  D ++ GF VP  +++ VN++A   D   WE+   + PERFL +  D     ++ 
Sbjct: 393 PRKAESDVEVLGFMVPKDTQVFVNVWAIGRDPNVWENSSRFKPERFLGKDIDLRGRDYEL 452

Query: 469 MAFGGGKRVCAGALKAMLISCTTIGKLVQEFEWKLREG----EEDKVDTLGLTARKLQPL 502
             FG G+R+C G   A+      +  L+  F+WKL  G    + D  +T GLT  K  PL
Sbjct: 453 TPFGAGRRICPGLPLAVKTVPLMLASLLYSFDWKLPNGVGSEDLDMDETFGLTLHKTNPL 509