Spg006966 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg006966 vs. NCBI nr
Match: XP_038875156.1 (WPP domain-interacting tail-anchored protein 2-like [Benincasa hispida])

HSP 1 Score: 978.0 bits (2527), Expect = 3.9e-281
Identity = 537/589 (91.17%), Postives = 566/589 (96.10%), Query Frame = 0

Query: 56  VGMMDENACNQESERLEMQELENTMKTLTKLELDLAYSSEKLMNLQVLFMCLLAQENDLE 115
           +GMMDENACN ES+RLEMQE+ENTMKTLTKLELDLAYSSEKLMNL VL MCLL QEND E
Sbjct: 1   MGMMDENACNHESQRLEMQEMENTMKTLTKLELDLAYSSEKLMNLHVLLMCLLTQENDFE 60

Query: 116 AMDLESDYIPDDSFGKVLAFDFLSGFLDSEVRELDSFMDTLVAEIVDVRGMISSCRHSSE 175
           AMDLE++YIPDDSFGKVLAFDFLSGFLDSEVRELDSFMD L AEIVD RGM+SSCR SSE
Sbjct: 61  AMDLENEYIPDDSFGKVLAFDFLSGFLDSEVRELDSFMDKLEAEIVDARGMVSSCRQSSE 120

Query: 176 VFNVVEEKLHDSEKSLIQSRQQILEVKMQSTKLQRIVLSSGNWRLEDPVMSSSQNDQV-L 235
           VF+VVEEKL DSEKSL+QS+QQILEVKMQS KLQRIVLSSGNWRLEDPVM SSQNDQV L
Sbjct: 121 VFSVVEEKLLDSEKSLVQSQQQILEVKMQSAKLQRIVLSSGNWRLEDPVM-SSQNDQVLL 180

Query: 236 NINGKSNTMTEQQRHVLRMLEKSLARELDLEKKLSESKQREEELRLKLHYTEQVALRMEE 295
           NINGKSNTMTEQQRH+LRMLEKSLARELDLEKKLSESKQREEELR+KLHYTEQVALRMEE
Sbjct: 181 NINGKSNTMTEQQRHILRMLEKSLARELDLEKKLSESKQREEELRMKLHYTEQVALRMEE 240

Query: 296 AAEVVWGRFLEADNSVEILTGISKELVGRLQLVQFNLHGSFQRENEIKAKFQDWTEQLKA 355
           AAEVVWGRFLEADNSVEIL GISKELVGRLQLVQFNLHGSFQRENE+K K QDWTEQLKA
Sbjct: 241 AAEVVWGRFLEADNSVEILMGISKELVGRLQLVQFNLHGSFQRENEVKTKCQDWTEQLKA 300

Query: 356 KEVAIQELEKRNAE-QIAKNAELDKLREEVKSLEEQLKESRLDLKSAYDSNEASQDQLIE 415
           KEVAIQ+LEKRNAE  IAKNAELDKLREEVKSLEEQLKESRLDLKSAYDSNEASQDQLIE
Sbjct: 301 KEVAIQKLEKRNAELLIAKNAELDKLREEVKSLEEQLKESRLDLKSAYDSNEASQDQLIE 360

Query: 416 MENLVESLKESICMAENRAESAEAKLTQLQETNLELTEEVTFLKDSASNKEKKVGSLEKQ 475
           MENLVESLKESIC++ENRAES EAKLTQLQETNLELTEEV+FLKDSASNKEKKVGSLEKQ
Sbjct: 361 MENLVESLKESICISENRAESVEAKLTQLQETNLELTEEVSFLKDSASNKEKKVGSLEKQ 420

Query: 476 LRELEIQLQHAKSSSEASQEQQNMLYSAIWDMETLIEDLKSKVSKAESKTDTAEEHCIIL 535
           LR+LEIQLQHAKSSSEASQEQQNMLYSAIWDMETLIEDLKSKVSKAESKTD+AEEHCIIL
Sbjct: 421 LRQLEIQLQHAKSSSEASQEQQNMLYSAIWDMETLIEDLKSKVSKAESKTDSAEEHCIIL 480

Query: 536 SETNFELNKELTSLKARVEFLEKALDQANSEKFTNANEINLSTKFIMDMVLQLAVERDRI 595
           SETNFELNKELTSLKARVEFLEKALDQAN EK+TNAN++NLS+KFIMDMVLQLAVERDRI
Sbjct: 481 SETNFELNKELTSLKARVEFLEKALDQANGEKYTNANKVNLSSKFIMDMVLQLAVERDRI 540

Query: 596 QSQLSILTNDNKSLIERLKNVKDDGSLITRDRGDYDEKERSAPKNDVNI 643
           QSQLSILTNDNK+LIE+L+ V+D  S+ITRDRGDYD+KERSAP+NDVNI
Sbjct: 541 QSQLSILTNDNKALIEKLRTVRDGASIITRDRGDYDKKERSAPENDVNI 588

BLAST of Spg006966 vs. NCBI nr
Match: KAG7016657.1 (WPP domain-interacting tail-anchored protein 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 961.8 bits (2485), Expect = 2.9e-276
Identity = 525/590 (88.98%), Postives = 561/590 (95.08%), Query Frame = 0

Query: 56  VGMMDENACNQESERLEMQELENTMKTLTKLELDLAYSSEKLMNLQVLFMCLLAQENDLE 115
           +GMMDENACNQES+ LEMQELE+TMKTLTKLEL+LAYSSEKL NL VL MCL+AQEND E
Sbjct: 1   MGMMDENACNQESQGLEMQELESTMKTLTKLELNLAYSSEKLTNLHVLLMCLMAQENDFE 60

Query: 116 AMDLESDYIPDDSFGKVLAFDFLSGFLDSEVRELDSFMDTLVAEIVDVRGMISSCRHSSE 175
            +DLE+D IPDD F KVLAFDFLSGFLDSEVRELDS MD+L AEIVD RGMISSCR SSE
Sbjct: 61  GVDLENDSIPDDLFEKVLAFDFLSGFLDSEVRELDSNMDSLEAEIVDSRGMISSCRQSSE 120

Query: 176 VFNVVEEKLHDSEKSLIQSRQQILEVKMQSTKLQRIVLSSGNWRLEDPVMSSSQNDQVLN 235
           VFNVVEEKL+DSE+S++QSRQQILEVKMQ  KLQR+VLSSGNWRLEDPVM SSQNDQVLN
Sbjct: 121 VFNVVEEKLNDSERSIVQSRQQILEVKMQLAKLQRMVLSSGNWRLEDPVM-SSQNDQVLN 180

Query: 236 INGKSNTMTEQQRHVLRMLEKSLARELDLEKKLSESKQREEELRLKLHYTEQVALRMEEA 295
           INGKSN +TEQQRH+LRMLEKSLARELDLEKKLSESKQREEELRLKLHYTEQV LRMEEA
Sbjct: 181 INGKSNPITEQQRHILRMLEKSLARELDLEKKLSESKQREEELRLKLHYTEQVTLRMEEA 240

Query: 296 AEVVWGRFLEADNSVEILTGISKELVGRLQLVQFNLHGSFQRENEIKAKFQDWTEQLKAK 355
           AEVVWGRFLEADNSVEIL GISKELVGRLQLVQFNLHGSFQRENEIKAKFQDWTEQLKAK
Sbjct: 241 AEVVWGRFLEADNSVEILMGISKELVGRLQLVQFNLHGSFQRENEIKAKFQDWTEQLKAK 300

Query: 356 EVAIQELEKRNAEQIAKNAELDKLREEVKSLEEQLKESRLDLKSAYDSNEASQDQLIEME 415
           EV+IQ+LEKRNA+ IAKNAELDKLREEVKSLE+Q+KES+LDLKSAYDSNEASQDQLIEME
Sbjct: 301 EVSIQKLEKRNADLIAKNAELDKLREEVKSLEDQVKESKLDLKSAYDSNEASQDQLIEME 360

Query: 416 NLVESLKESICMAENRAESAEAKLTQLQETNLELTEEVTFLKDSASNKEKKVGSLEKQLR 475
           NL+ESLKESIC++ENRAE+ EAKLTQLQETNLELTEEV+FLKDSASNKEKKVGSLEKQLR
Sbjct: 361 NLIESLKESICISENRAENVEAKLTQLQETNLELTEEVSFLKDSASNKEKKVGSLEKQLR 420

Query: 476 ELEIQLQHAKSSSEASQEQQNMLYSAIWDMETLIEDLKSKVSKAESKTDTAEEHCIILSE 535
           ELEIQLQHAKSSSEASQEQQNMLYSAIWDMETLIE+LKSKVSKAESKTD+AEEHCIILSE
Sbjct: 421 ELEIQLQHAKSSSEASQEQQNMLYSAIWDMETLIEELKSKVSKAESKTDSAEEHCIILSE 480

Query: 536 TNFELNKELTSLKARVEFLEKALDQANSEKFTNANEINLSTKFIMDMVLQLAVERDRIQS 595
           TNFELNKEL SLK R+EFLEKALDQA S KFTNANEINLSTKFIMDMVLQLAVERDRIQS
Sbjct: 481 TNFELNKELASLKTRIEFLEKALDQARSAKFTNANEINLSTKFIMDMVLQLAVERDRIQS 540

Query: 596 QLSILTNDNKSLIERLKNVKDDGSLITRDRGDYDEKERSAPKNDVNILAQ 646
           QLS+LTNDNK+LIERLKNVK+  S++TRDRGDYDE++RSAP  DVNILAQ
Sbjct: 541 QLSLLTNDNKALIERLKNVKNGASVVTRDRGDYDEEQRSAPMKDVNILAQ 589

BLAST of Spg006966 vs. NCBI nr
Match: XP_022994072.1 (WPP domain-interacting tail-anchored protein 2-like [Cucurbita maxima] >XP_022994073.1 WPP domain-interacting tail-anchored protein 2-like [Cucurbita maxima])

HSP 1 Score: 957.2 bits (2473), Expect = 7.1e-275
Identity = 524/590 (88.81%), Postives = 559/590 (94.75%), Query Frame = 0

Query: 56  VGMMDENACNQESERLEMQELENTMKTLTKLELDLAYSSEKLMNLQVLFMCLLAQENDLE 115
           +GMMDENACNQE + LEMQELE++MKTLTKLEL+LAYSSEKL NL VL MCL+AQEND E
Sbjct: 1   MGMMDENACNQEYQGLEMQELESSMKTLTKLELNLAYSSEKLTNLHVLLMCLMAQENDFE 60

Query: 116 AMDLESDYIPDDSFGKVLAFDFLSGFLDSEVRELDSFMDTLVAEIVDVRGMISSCRHSSE 175
            +DLE+D IPDD F KVLAFDFLSGFLDSEVRELDS MD+L AEIVD RGMISSCR SSE
Sbjct: 61  GVDLENDSIPDDLFEKVLAFDFLSGFLDSEVRELDSNMDSLEAEIVDSRGMISSCRQSSE 120

Query: 176 VFNVVEEKLHDSEKSLIQSRQQILEVKMQSTKLQRIVLSSGNWRLEDPVMSSSQNDQVLN 235
           VFNVVEEKL+DSE+S++QSRQQILEVKMQ  KLQR+VLSSGNWRLEDPVM SSQNDQVLN
Sbjct: 121 VFNVVEEKLNDSERSIVQSRQQILEVKMQLAKLQRMVLSSGNWRLEDPVM-SSQNDQVLN 180

Query: 236 INGKSNTMTEQQRHVLRMLEKSLARELDLEKKLSESKQREEELRLKLHYTEQVALRMEEA 295
           INGKSN +TEQQRH+LRMLEKSLARELDLEKKLSESKQREEELRLKLHYTEQV LRMEEA
Sbjct: 181 INGKSNPITEQQRHILRMLEKSLARELDLEKKLSESKQREEELRLKLHYTEQVTLRMEEA 240

Query: 296 AEVVWGRFLEADNSVEILTGISKELVGRLQLVQFNLHGSFQRENEIKAKFQDWTEQLKAK 355
           AEVVWGRFLEADNSVEIL GISKELVGRLQLVQFNLHGSFQRENEIKAKFQDW EQLKAK
Sbjct: 241 AEVVWGRFLEADNSVEILMGISKELVGRLQLVQFNLHGSFQRENEIKAKFQDWMEQLKAK 300

Query: 356 EVAIQELEKRNAEQIAKNAELDKLREEVKSLEEQLKESRLDLKSAYDSNEASQDQLIEME 415
           EV+IQ+LEKRNA+ IAKNAELDKLREEVKSLEEQLKES+LDLKS YDSNEASQDQLIEME
Sbjct: 301 EVSIQKLEKRNADLIAKNAELDKLREEVKSLEEQLKESKLDLKSVYDSNEASQDQLIEME 360

Query: 416 NLVESLKESICMAENRAESAEAKLTQLQETNLELTEEVTFLKDSASNKEKKVGSLEKQLR 475
           NL+ESLKESIC++ENRAE+ EAKLTQLQETNLELTEEV+FLKDSASNKEKKVGSLEKQLR
Sbjct: 361 NLIESLKESICISENRAENVEAKLTQLQETNLELTEEVSFLKDSASNKEKKVGSLEKQLR 420

Query: 476 ELEIQLQHAKSSSEASQEQQNMLYSAIWDMETLIEDLKSKVSKAESKTDTAEEHCIILSE 535
           ELEIQLQHAKSSSEASQEQQNMLYSAIWDMETLIE+LKSKVSKAESKTD+AEEHCIILSE
Sbjct: 421 ELEIQLQHAKSSSEASQEQQNMLYSAIWDMETLIEELKSKVSKAESKTDSAEEHCIILSE 480

Query: 536 TNFELNKELTSLKARVEFLEKALDQANSEKFTNANEINLSTKFIMDMVLQLAVERDRIQS 595
           TNFELNKEL SLKAR+EFLEKALDQA S KFTNANEINLSTKFIMDMVLQLAVERDRIQS
Sbjct: 481 TNFELNKELASLKARIEFLEKALDQARSAKFTNANEINLSTKFIMDMVLQLAVERDRIQS 540

Query: 596 QLSILTNDNKSLIERLKNVKDDGSLITRDRGDYDEKERSAPKNDVNILAQ 646
           QLSILTNDNK+LIERLKNVK+  S++TRDRG+YDE++RSAP  DVNILAQ
Sbjct: 541 QLSILTNDNKALIERLKNVKNGASVVTRDRGEYDEEQRSAPMKDVNILAQ 589

BLAST of Spg006966 vs. NCBI nr
Match: XP_022146430.1 (WPP domain-interacting tail-anchored protein 2-like [Momordica charantia] >XP_022146431.1 WPP domain-interacting tail-anchored protein 2-like [Momordica charantia] >XP_022146432.1 WPP domain-interacting tail-anchored protein 2-like [Momordica charantia])

HSP 1 Score: 955.7 bits (2469), Expect = 2.1e-274
Identity = 530/591 (89.68%), Postives = 554/591 (93.74%), Query Frame = 0

Query: 59  MDEN----ACNQESERLEMQELENTMKTLTKLELDLAYSSEKLMNLQVLFMCLLAQENDL 118
           MDEN      N+ESERLEMQELENTMKTLTKLELDLAY SEKLMNL VL MCLLAQEND 
Sbjct: 1   MDENPGIYVANEESERLEMQELENTMKTLTKLELDLAYCSEKLMNLHVLLMCLLAQENDF 60

Query: 119 EAMDLESDYIPDDSFGKVLAFDFLSGFLDSEVRELDSFMDTLVAEIVDVRGMISSCRHSS 178
           EAMDLE+DYIPDDSFGKVLAFDFLSGFLDSEVRELDSFM+ L  EI+  R M+SSCR SS
Sbjct: 61  EAMDLENDYIPDDSFGKVLAFDFLSGFLDSEVRELDSFMNMLEEEIIGARRMVSSCRQSS 120

Query: 179 EVFNVVEEKLHDSEKSLIQSRQQILEVKMQSTKLQRIVLSSGNWRLEDPVMSSSQNDQVL 238
           EVFNVVEEKLHDSEKSL+QSR+QILEV MQSTKLQRIVLSSGNWRLEDPV+SS+ NDQVL
Sbjct: 121 EVFNVVEEKLHDSEKSLVQSRKQILEVMMQSTKLQRIVLSSGNWRLEDPVLSSN-NDQVL 180

Query: 239 NINGKSNTMTEQQRHVLRMLEKSLARELDLEKKLSESKQREEELRLKLHYTEQVALRMEE 298
           NI GKSNTMTEQQRH+LRMLEKSLARELDLEKKLSESKQREEELRLKLHYTEQVALRMEE
Sbjct: 181 NIYGKSNTMTEQQRHILRMLEKSLARELDLEKKLSESKQREEELRLKLHYTEQVALRMEE 240

Query: 299 AAEVVWGRFLEADNSVEILTGISKELVGRLQLVQFNLHGSFQRENEIKAKFQDWTEQLKA 358
           AAEVVWGRFLEADNSVE+L GISKELVGRLQLVQFNLHGSFQRENE+K+KFQDWTEQLKA
Sbjct: 241 AAEVVWGRFLEADNSVEVLMGISKELVGRLQLVQFNLHGSFQRENEMKSKFQDWTEQLKA 300

Query: 359 KEVAIQELEKRNAEQIAKNAELDKLREEVKSLEEQLKESRLDLKSAYDSNEASQDQLIEM 418
           KEVAIQELEKRNAE+IAK+AELDKLREEVKSLEEQLKESRLDLK+A++S EASQDQLIEM
Sbjct: 301 KEVAIQELEKRNAEKIAKSAELDKLREEVKSLEEQLKESRLDLKNAFESEEASQDQLIEM 360

Query: 419 ENLVESLKESICMAENRAESAEAKLTQLQETNLELTEEVTFLKDSASNKEKKVGSLEKQL 478
           ENLVESLKE ICMAENRAE AEAKL QLQETNLELTEEV FLKDSAS+KEKKVGSLEKQL
Sbjct: 361 ENLVESLKEGICMAENRAEGAEAKLAQLQETNLELTEEVNFLKDSASSKEKKVGSLEKQL 420

Query: 479 RELEIQLQHAKSSSEASQEQQNMLYSAIWDMETLIEDLKSKVSKAESKTDTAEEHCIILS 538
           RE EIQLQHAKSSSEASQEQQNMLYSAIWDMETLIEDLKSKVSKAESKTDTAEEHCIILS
Sbjct: 421 REQEIQLQHAKSSSEASQEQQNMLYSAIWDMETLIEDLKSKVSKAESKTDTAEEHCIILS 480

Query: 539 ETNFELNKELTSLKARVEFLEKALDQANSEKFTNANEINLSTKFIMDMVLQLAVERDRIQ 598
           E NFELNKELTSLKARVEFLEKALDQANSEK +NANEIN STKFIMDMVLQLAVERDRIQ
Sbjct: 481 EANFELNKELTSLKARVEFLEKALDQANSEKCSNANEINSSTKFIMDMVLQLAVERDRIQ 540

Query: 599 SQLSILTNDNKSLIERLKNVKDDGSLITRDRGDYDEKERSAPKNDVNILAQ 646
            QLSILTNDNK+LIERLKNVKD  S+I  D GDYD+KE+SAPKNDVN LAQ
Sbjct: 541 RQLSILTNDNKALIERLKNVKDGASVIICDGGDYDKKEQSAPKNDVNKLAQ 590

BLAST of Spg006966 vs. NCBI nr
Match: XP_008466053.1 (PREDICTED: WPP domain-interacting tail-anchored protein 2-like [Cucumis melo] >XP_008466062.1 PREDICTED: WPP domain-interacting tail-anchored protein 2-like [Cucumis melo])

HSP 1 Score: 954.5 bits (2466), Expect = 4.6e-274
Identity = 521/591 (88.16%), Postives = 558/591 (94.42%), Query Frame = 0

Query: 56  VGMMDENACNQESERLEMQELENTMKTLTKLELDLAYSSEKLMNLQVLFMCLLAQENDLE 115
           +GMMDEN CN+ES+RLEMQE+E TMKTLTKLELDLAYSSEKLMNL VL MCLLAQEND E
Sbjct: 1   MGMMDENGCNRESQRLEMQEMEYTMKTLTKLELDLAYSSEKLMNLHVLLMCLLAQENDFE 60

Query: 116 AMDLESDYIPDDSFGKVLAFDFLSGFLDSEVRELDSFMDTLVAEIVDVRGMI-SSCRHSS 175
           AMDLE+DYIPDDSFGK+L FDFLSGFLDSEVRELD+FM+TL AEIVD RGM  SSC  S+
Sbjct: 61  AMDLENDYIPDDSFGKLLVFDFLSGFLDSEVRELDTFMNTLEAEIVDARGMASSSCLQST 120

Query: 176 EVFNVVEEKLHDSEKSLIQSRQQILEVKMQSTKLQRIVLSSGNWRLEDPVMSSSQNDQVL 235
           EVF+V+E KL DSEKSL+QSR+QILEVKMQSTKLQRIVLS GNWRLEDP+M SSQNDQV 
Sbjct: 121 EVFSVLEGKLIDSEKSLVQSRKQILEVKMQSTKLQRIVLSRGNWRLEDPMM-SSQNDQVF 180

Query: 236 NINGKSNTMTEQQRHVLRMLEKSLARELDLEKKLSESKQREEELRLKLHYTEQVALRMEE 295
           NINGKSNTMTEQQRH+LRMLEKSLARELDLEK+LSESKQREEEL++KLHYTEQVALRMEE
Sbjct: 181 NINGKSNTMTEQQRHILRMLEKSLARELDLEKQLSESKQREEELKMKLHYTEQVALRMEE 240

Query: 296 AAEVVWGRFLEADNSVEILTGISKELVGRLQLVQFNLHGSFQRENEIKAKFQDWTEQLKA 355
            AEVVWGRFLEADNSVEIL GISKELVGRLQLVQFNLHGSFQRE+EIK KFQDWTEQL A
Sbjct: 241 TAEVVWGRFLEADNSVEILMGISKELVGRLQLVQFNLHGSFQREHEIKTKFQDWTEQLNA 300

Query: 356 KEVAIQELEKRNAEQIAKNAELDKLREEVKSLEEQLKESRLDLKSAYDSNEASQDQLIEM 415
           KEVAIQ+LEKRNAE IAKNAELDKLREEVKSLEEQLKE RLDLK+AY+SNEASQDQLIEM
Sbjct: 301 KEVAIQKLEKRNAELIAKNAELDKLREEVKSLEEQLKELRLDLKTAYESNEASQDQLIEM 360

Query: 416 ENLVESLKESICMAENRAESAEAKLTQLQETNLELTEEVTFLKDSASNKEKKVGSLEKQL 475
           ENLVESLKESIC++ENRAES E KLTQLQETNLELTEEV+FLKDSAS+KEKKVGSLEKQL
Sbjct: 361 ENLVESLKESICISENRAESVETKLTQLQETNLELTEEVSFLKDSASSKEKKVGSLEKQL 420

Query: 476 RELEIQLQHAKSSSEASQEQQNMLYSAIWDMETLIEDLKSKVSKAESKTDTAEEHCIILS 535
           RELEIQLQHAKSSSEASQEQQNMLYSAIWDMETLIEDLKSKVSKAESKTD+AEEHCIILS
Sbjct: 421 RELEIQLQHAKSSSEASQEQQNMLYSAIWDMETLIEDLKSKVSKAESKTDSAEEHCIILS 480

Query: 536 ETNFELNKELTSLKARVEFLEKALDQANSEKFTNANEINLSTKFIMDMVLQLAVERDRIQ 595
           ETNFELNKELTSLK RVEFLEKALDQAN EK+ NANEINLS+KFIMDMVLQLAVERDR+Q
Sbjct: 481 ETNFELNKELTSLKGRVEFLEKALDQANGEKYANANEINLSSKFIMDMVLQLAVERDRVQ 540

Query: 596 SQLSILTNDNKSLIERLKNVKDDGSLITRDRGDYDEKERSAPKNDVNILAQ 646
            QLSILTNDNK+LIE+LKN++D  S++TRDR DYDE+ERSAPK DVNILAQ
Sbjct: 541 RQLSILTNDNKALIEKLKNIRDGASIVTRDREDYDEEERSAPKKDVNILAQ 590

BLAST of Spg006966 vs. ExPASy Swiss-Prot
Match: A8MQR0 (WPP domain-interacting tail-anchored protein 2 OS=Arabidopsis thaliana OX=3702 GN=WIT2 PE=1 SV=1)

HSP 1 Score: 474.9 bits (1221), Expect = 1.4e-132
Identity = 293/537 (54.56%), Postives = 384/537 (71.51%), Query Frame = 0

Query: 74  QELENTMKTLTKLELDLAYSSEKLMNLQVLFMCLLAQENDLEAM-DLESDYIPDDSFGKV 133
           +E +  ++ LTKLE+D AY+SEKLMNL VL M LLA +NDLE M  L+S      SF K 
Sbjct: 18  EEEQEMLRMLTKLEIDSAYTSEKLMNLHVLLMHLLAWDNDLEGMGTLDSS---PASFEKA 77

Query: 134 LAFDFLSGFLDSEVRELDSFMDTLVAEIVDVRGMISSCRHSSEVFNVVEEKLHDSEKSLI 193
           L FD L G L+SEV+E+D  +D L A+IVD    ISSC+H + +  V+E KL +S +SL 
Sbjct: 78  LTFDLLCGILESEVKEVDEVLDVLEAQIVDTSYKISSCKHGNYI--VIEGKLGESAESLK 137

Query: 194 QSRQQILEVKMQSTKLQRIVLSSGNWRLEDPVMSSSQNDQVLNINGKSNTMTEQQRH--V 253
           QSR Q+ E+ +Q  +L+R +    N         +S+N++ + +  K        RH   
Sbjct: 138 QSRGQVSEITLQLAQLRRTLHYIRN--------GTSENEESVELRQKYALKPSDLRHKNA 197

Query: 254 LRMLEKSLARELDLEKKLSESKQREEELRLKLHYTEQVALRMEEAAEVVWGRFLEADNSV 313
           LRMLEKSL+REL+LEKKL E +Q EE+L+LKLHYTE+V+ RMEEA+E +WGRFLEADNS 
Sbjct: 198 LRMLEKSLSRELELEKKLMEFQQNEEQLKLKLHYTEEVSSRMEEASEFIWGRFLEADNSS 257

Query: 314 EILTGISKELVGRLQLVQFNLHGSFQRENEIKAKFQDWTEQLKAKEVAIQELEKRNAEQI 373
           E+LTGISKELVGRLQ++QF+L+GS QRE+E+K+K +D T QL+AK++ +Q+LE   +E  
Sbjct: 258 EVLTGISKELVGRLQILQFSLNGSAQRESELKSKLEDCTVQLEAKDLLVQKLEGTISENS 317

Query: 374 AKNAELDKLREEVKSLEEQLKESRLDLKSAYDSNEASQDQLIEMENLVESLKESICMAEN 433
              +E+  LRE VKS E++LK + L+LKS   S +     L EMEN  ES+KE++  AE+
Sbjct: 318 EIVSEVLTLREYVKSAEQKLKNTDLELKSVNASKQEILVHLAEMENANESVKENLFEAES 377

Query: 434 RAESAEAKLTQLQETNLELTEEVTFLKDSASNKEKKVGSLEKQLRELEIQLQHAKSSSEA 493
           RAES EAK+ +L   NLELTEE+ FLKD+   K KKV SLEKQ+RELE+Q+Q++K SSEA
Sbjct: 378 RAESGEAKIKELDAANLELTEELNFLKDADDKKTKKVNSLEKQVRELEVQVQNSKVSSEA 437

Query: 494 SQEQQNMLYSAIWDMETLIEDLKSKVSKAESKTDTAEEHCIILSETNFELNKELTSLKAR 553
           +QEQQNMLYSAIWDMETLIEDLKSK SKAES+T+T EE CI+LS TN ELNK+++ L+ +
Sbjct: 438 NQEQQNMLYSAIWDMETLIEDLKSKASKAESRTETVEEQCIVLSTTNSELNKDVSFLRQK 497

Query: 554 VEFLEKALDQANSEKFTNANEINLSTKFIMDMVLQLAVERDRIQSQLSILTNDNKSL 608
            + LE  LD AN+EK   A EI    K +MDM+LQL+ ER+RIQ QL  L  +NK L
Sbjct: 498 AKSLEAMLDLANNEKERYAQEITTRNKVLMDMMLQLSSERERIQEQLYSLAKENKIL 541

BLAST of Spg006966 vs. ExPASy Swiss-Prot
Match: Q8L7E5 (WPP domain-interacting tail-anchored protein 1 OS=Arabidopsis thaliana OX=3702 GN=WIT1 PE=1 SV=2)

HSP 1 Score: 348.6 bits (893), Expect = 1.5e-94
Identity = 250/604 (41.39%), Postives = 365/604 (60.43%), Query Frame = 0

Query: 75  ELENTMKTLTKLELDLAYSSEKLMNLQVLFMCLLAQENDLEAM---DLESDYIP------ 134
           +L +  + LTK+ELD A+ SEKL+NL +L M L  +END E+      E +  P      
Sbjct: 44  QLSSAGEVLTKVELDFAFVSEKLVNLSLLTMQLGTRENDFESFVSKKEEDEEEPSSNVDD 103

Query: 135 -DDSFGKVLAFDFLSGFLDSEVRELDSFMDTLVAEIVDVRGMISSCRHSSEVFNVVEEKL 194
            DDS  K L FD LS  L+SEV+EL+S +  L  EI   R MIS  +H  E F  +E KL
Sbjct: 104 DDDSAEKALEFDLLSSILNSEVKELESLLGFLQNEIQSARVMISPFQHDGEAFLDLEGKL 163

Query: 195 HDSEKSLIQSRQQILEVKMQSTKLQRI---VLSSGNWRLEDPVMSSSQND-QVLNINGKS 254
           +D+E+SL Q  +Q++E+K QS+  QR+   +   G+W       S SQND +  +++ K 
Sbjct: 164 NDTEQSLGQLMEQVVEMKKQSSNFQRLSSGLDEQGSW--SGGQTSVSQNDGEFGDLSAKI 223

Query: 255 NTMT-EQQRHVLRMLEKSLARELDLEKKLSESKQREEELRLKLHYTEQVALRMEEAAEVV 314
           N  T +QQR+VLRMLEKSLA+E++LEKKLSES+  E EL +KL+ +EQ  + MEE  E  
Sbjct: 224 NMQTADQQRNVLRMLEKSLAKEMELEKKLSESRNTERELEMKLYSSEQDVVYMEEVTEDA 283

Query: 315 WGRFLEADNSVEILTGISKELVGRLQLVQFNLHGSFQRENEIKAKFQDWTEQLKAKEVAI 374
           + R+LEADN+ E+  G SKE+ G+LQ++QFNL GSF+RE+ +K+K  D  E+L+AKE A+
Sbjct: 284 FSRWLEADNAAEVFKGTSKEMSGKLQILQFNLSGSFKREDNLKSKLVDSKERLEAKECAL 343

Query: 375 QELEKRNA----------------------EQIAKNAELDKLREEVKSLEEQLKESRLDL 434
            +L+  NA                      + I  N E   L E+V SLEEQL E  +  
Sbjct: 344 HKLDSSNARLADFLVAQTEGLKESLQEAEEKLILLNTENSTLSEKVSSLEEQLNEYGIQT 403

Query: 435 KSAYDSNEASQDQLIEMENLVESLKESICMAENRAESAEAKLTQLQETNLELTEEVTFLK 494
           +   D++  S   + ++E + E LK+ +   E RAE  E+K   L+E+  EL +E+   +
Sbjct: 404 E---DADATSGALITDLERINEELKDKLAKTEARAEETESKCKILEESKKELQDELGNFR 463

Query: 495 DSASNKEKKVGSLEKQLRELEIQLQHAKSSSEASQEQQNMLYSAIWDMETLIEDLKSKVS 554
           D       K+ SLEK LR+ ++QL+HA ++ EAS+E+QN+LYS + DME +IEDLKSKV 
Sbjct: 464 DKGFTIH-KLASLEKHLRDSDLQLEHAVAAVEASKEKQNLLYSTVSDMEDVIEDLKSKVL 523

Query: 555 KAESKTDTAEEHCIILSETNFELNKELTSLKARVEFLEKALDQANSEKFTNANEINLSTK 614
           KAE++ D  EE  I++SE+N E+N+EL   K R++  EK L QA   K   A +I +  K
Sbjct: 524 KAENRADITEEKLIMVSESNAEVNEELKFFKGRLKEGEKYLQQAEERKLRTAKDIGVHNK 583

Query: 615 FIMDMVLQLAVERDRIQSQLSILTNDNKSLIERLKNVKDDGSLITRDRGDYDEKERSAPK 642
            +  +V+QLA ER+R+  Q++ L+ +N  L+ +LK V   G +   + G+  E    +PK
Sbjct: 584 IMKKLVMQLAAERERLHKQITNLSRENCVLMVKLKKVGKTGYM---ESGNGSE---VSPK 635

BLAST of Spg006966 vs. ExPASy Swiss-Prot
Match: P35579 (Myosin-9 OS=Homo sapiens OX=9606 GN=MYH9 PE=1 SV=4)

HSP 1 Score: 53.5 bits (127), Expect = 1.0e-05
Identity = 98/424 (23.11%), Postives = 185/424 (43.63%), Query Frame = 0

Query: 233  VLNINGKSNTMTEQQRHVLRMLEKSLARELDLEKKLSESKQREEELRL-KLHYTEQVALR 292
            +L ++ +   M  ++  ++++ EK LA     E +L+E +  + +L   KL   EQ+   
Sbjct: 837  LLQVSRQEEEMMAKEEELVKVREKQLA----AENRLTEMETLQSQLMAEKLQLQEQLQAE 896

Query: 293  MEEAAEVVWGRFLEADNSVEILTGISKELVGRLQLVQFNLHGSFQRENEIKAKFQDWTEQ 352
             E  AE    R        E L  I  +L  R++  +         + +++   Q+  EQ
Sbjct: 897  TELCAEAEELRARLTAKKQE-LEEICHDLEARVEEEEERCQHLQAEKKKMQQNIQELEEQ 956

Query: 353  LKAKEVAIQELEKRNAEQIAKNAELDKLREEVKSLEEQ----LKESRL--DLKSAYDSN- 412
            L+ +E A Q+L+    E++   A+L KL EE   LE+Q     KE +L  D  + + +N 
Sbjct: 957  LEEEESARQKLQ---LEKVTTEAKLKKLEEEQIILEDQNCKLAKEKKLLEDRIAEFTTNL 1016

Query: 413  ---EASQDQLIEMENLVESLKESICMAENRAESAEAKLTQLQETNLELTEEVTFLKDSAS 472
               E     L +++N  E++   I   E R    E +  +L++T  +L  + T L D  +
Sbjct: 1017 TEEEEKSKSLAKLKNKHEAM---ITDLEERLRREEKQRQELEKTRRKLEGDSTDLSDQIA 1076

Query: 473  NKEKKVGSLEKQLRELEIQLQHAKSSSEASQEQQNMLYSAIWDMETLIEDLKSKVSKAES 532
              + ++  L+ QL + E +LQ A +  E    Q+NM    I ++E+ I +L+  +    +
Sbjct: 1077 ELQAQIAELKMQLAKKEEELQAALARVEEEAAQKNMALKKIRELESQISELQEDLESERA 1136

Query: 533  KTDTAEEHCIILSETNFELNKELTSLKARVEFLEKALDQANSEKFTNANEINLSTKFIMD 592
              + AE+          +L +EL +LK  +E    +       +     E+N+  K + +
Sbjct: 1137 SRNKAEKQ-------KRDLGEELEALKTELEDTLDSTAAQQELRSKREQEVNILKKTLEE 1196

Query: 593  MVLQLAVERDRIQSQLSILTNDNKSLIERLKNVKDDGSLITRDRGDYDEKERSAPKNDVN 646
                   +   ++ + S    +    +E+ K VK +     +      E ER    N+V 
Sbjct: 1197 EAKTHEAQIQEMRQKHSQAVEELAEQLEQTKRVKANLEKAKQTL----ENERGELANEVK 1238

BLAST of Spg006966 vs. ExPASy Swiss-Prot
Match: P14105 (Myosin-9 OS=Gallus gallus OX=9031 GN=MYH9 PE=2 SV=1)

HSP 1 Score: 51.6 bits (122), Expect = 3.8e-05
Identity = 98/424 (23.11%), Postives = 185/424 (43.63%), Query Frame = 0

Query: 233  VLNINGKSNTMTEQQRHVLRMLEKSLARELDLEKKLSESKQREEELRL-KLHYTEQVALR 292
            +L ++ +   M  ++  ++++ EK LA     E +LSE +  + +L   K+   EQ+   
Sbjct: 837  LLQVSRQEEEMMAKEEELIKVKEKQLA----AENRLSEMETFQAQLMAEKMQLQEQLQAE 896

Query: 293  MEEAAEVVWGRFLEADNSVEILTGISKELVGRLQLVQFNLHGSFQRENEIKAKFQDWTEQ 352
             E  AE    R        E L  I  +L  R++  +         + +++   Q+  EQ
Sbjct: 897  AELCAEAEEIRARLTAKKQE-LEEICHDLEARVEEEEERCQHLQAEKKKMQQNIQELEEQ 956

Query: 353  LKAKEVAIQELEKRNAEQIAKNAELDKLREEVKSLEEQ----LKESRL--DLKSAYDSN- 412
            L+ +E A Q+L+    E++   A+L KL E+V  LE+Q     KE +L  D  S + +N 
Sbjct: 957  LEEEESARQKLQ---LEKVTTEAKLKKLEEDVIVLEDQNLKLAKEKKLLEDRMSEFTTNL 1016

Query: 413  ---EASQDQLIEMENLVESLKESICMAENRAESAEAKLTQLQETNLELTEEVTFLKDSAS 472
               E     L +++N  E++   I   E R    E +  +L++T  +L  + + L D  +
Sbjct: 1017 TEEEEKSKSLAKLKNKHEAM---ITDLEERLRREEKQRQELEKTRRKLEGDSSDLHDQIA 1076

Query: 473  NKEKKVGSLEKQLRELEIQLQHAKSSSEASQEQQNMLYSAIWDMETLIEDLKSKVSKAES 532
              + ++  L+ QL + E +LQ A +  E    Q+NM    I ++E+ I +L+  +    +
Sbjct: 1077 ELQAQIAELKIQLSKKEEELQAALARVEEEAAQKNMALKKIRELESQITELQEDLESERA 1136

Query: 533  KTDTAEEHCIILSETNFELNKELTSLKARVEFLEKALDQANSEKFTNANEINLSTKFIMD 592
              + AE+          +L +EL +LK  +E    +       +     E+ +  K + D
Sbjct: 1137 SRNKAEKQ-------KRDLGEELEALKTELEDTLDSTAAQQELRSKREQEVTVLKKTLED 1196

Query: 593  MVLQLAVERDRIQSQLSILTNDNKSLIERLKNVKDDGSLITRDRGDYDEKERSAPKNDVN 646
                   +   ++ + S    +    +E+ K VK +            E ER+   N+V 
Sbjct: 1197 EAKTHEAQIQEMRQKHSQAIEELAEQLEQTKRVKAN----LEKAKQALESERAELSNEVK 1238

BLAST of Spg006966 vs. ExPASy Swiss-Prot
Match: P05659 (Myosin-2 heavy chain, non muscle OS=Acanthamoeba castellanii OX=5755 PE=3 SV=1)

HSP 1 Score: 51.2 bits (121), Expect = 5.0e-05
Identity = 86/378 (22.75%), Postives = 166/378 (43.92%), Query Frame = 0

Query: 247  QRHVLRMLEKSLARELDLEKKLSESKQREEELRLKLHYTEQVALRMEEAAEVVWGRFLEA 306
            QR+  + ++    +  DLEK+L+  K    +L  +    E+ A ++E+    +  + L+ 
Sbjct: 851  QRNFQKEIDDLKKQVKDLEKELAALKDANAKLDKEKQLAEEDADKLEKDLAALKLKILD- 910

Query: 307  DNSVEILTGISKELVGRLQLVQFNLHGSFQRENEIKAKFQDWTEQLKAKEVAIQELEKRN 366
                  L G   +L     L+Q  + G  +   E  +   D  EQ +  E    EL+   
Sbjct: 911  ------LEGEKADLEEDNALLQKKVAGLEEELQEETSASNDILEQKRKLEAEKGELKASL 970

Query: 367  AEQIAKNAELDKLREEVKSLEEQLKESRLDLKSAYDSNEASQDQLIEMENLVESLKESIC 426
             E+      L + + +V+S   +L++   D  +A+DS +  ++   ++   +   K+++ 
Sbjct: 971  EEEERNRKALQEAKTKVESERNELQDKYEDEAAAHDSLKKKEE---DLSRELRETKDALA 1030

Query: 427  MAENRAESAEAKLTQLQETNLELTEEVTFLKDSASNKEKKVGSLEKQLRELEIQLQHAKS 486
             AEN +E+  +KL   +    ++  E+  +  +    EK   SLE++L +   QL+  KS
Sbjct: 1031 DAENISETLRSKLKNTERGADDVRNELDDVTATKLQLEKTKKSLEEELAQTRAQLEEEKS 1090

Query: 487  SSEASQEQQNMLYSAIWDMETLIEDLKSKVSKAESKTDTAEEHCIILSETNFELNKELTS 546
              EA+  +   L   + D  + ++ LKSK+S AE    TA++    L E   +L  E T 
Sbjct: 1091 GKEAASSKAKQLGQQLEDARSEVDSLKSKLSAAEKSLKTAKDQNRDLDE---QLEDERT- 1150

Query: 547  LKARVEFLEKALDQANSEKFTNANEINLSTKFIMDMVLQLAVERDRIQSQLSILTNDNKS 606
            ++A V+  +KAL+   +E       ++            L  + D  + +L         
Sbjct: 1151 VRANVDKQKKALEAKLTELEDQVTALDGQKNAAAAQAKTLKTQVDETKRRLEEAEASAAR 1210

Query: 607  LIERLKNVKDDGSLITRD 625
            L +  KN  D+ + +T D
Sbjct: 1211 LEKERKNALDEVAQLTAD 1214

BLAST of Spg006966 vs. ExPASy TrEMBL
Match: A0A6J1K445 (WPP domain-interacting tail-anchored protein 2-like OS=Cucurbita maxima OX=3661 GN=LOC111489874 PE=4 SV=1)

HSP 1 Score: 957.2 bits (2473), Expect = 3.5e-275
Identity = 524/590 (88.81%), Postives = 559/590 (94.75%), Query Frame = 0

Query: 56  VGMMDENACNQESERLEMQELENTMKTLTKLELDLAYSSEKLMNLQVLFMCLLAQENDLE 115
           +GMMDENACNQE + LEMQELE++MKTLTKLEL+LAYSSEKL NL VL MCL+AQEND E
Sbjct: 1   MGMMDENACNQEYQGLEMQELESSMKTLTKLELNLAYSSEKLTNLHVLLMCLMAQENDFE 60

Query: 116 AMDLESDYIPDDSFGKVLAFDFLSGFLDSEVRELDSFMDTLVAEIVDVRGMISSCRHSSE 175
            +DLE+D IPDD F KVLAFDFLSGFLDSEVRELDS MD+L AEIVD RGMISSCR SSE
Sbjct: 61  GVDLENDSIPDDLFEKVLAFDFLSGFLDSEVRELDSNMDSLEAEIVDSRGMISSCRQSSE 120

Query: 176 VFNVVEEKLHDSEKSLIQSRQQILEVKMQSTKLQRIVLSSGNWRLEDPVMSSSQNDQVLN 235
           VFNVVEEKL+DSE+S++QSRQQILEVKMQ  KLQR+VLSSGNWRLEDPVM SSQNDQVLN
Sbjct: 121 VFNVVEEKLNDSERSIVQSRQQILEVKMQLAKLQRMVLSSGNWRLEDPVM-SSQNDQVLN 180

Query: 236 INGKSNTMTEQQRHVLRMLEKSLARELDLEKKLSESKQREEELRLKLHYTEQVALRMEEA 295
           INGKSN +TEQQRH+LRMLEKSLARELDLEKKLSESKQREEELRLKLHYTEQV LRMEEA
Sbjct: 181 INGKSNPITEQQRHILRMLEKSLARELDLEKKLSESKQREEELRLKLHYTEQVTLRMEEA 240

Query: 296 AEVVWGRFLEADNSVEILTGISKELVGRLQLVQFNLHGSFQRENEIKAKFQDWTEQLKAK 355
           AEVVWGRFLEADNSVEIL GISKELVGRLQLVQFNLHGSFQRENEIKAKFQDW EQLKAK
Sbjct: 241 AEVVWGRFLEADNSVEILMGISKELVGRLQLVQFNLHGSFQRENEIKAKFQDWMEQLKAK 300

Query: 356 EVAIQELEKRNAEQIAKNAELDKLREEVKSLEEQLKESRLDLKSAYDSNEASQDQLIEME 415
           EV+IQ+LEKRNA+ IAKNAELDKLREEVKSLEEQLKES+LDLKS YDSNEASQDQLIEME
Sbjct: 301 EVSIQKLEKRNADLIAKNAELDKLREEVKSLEEQLKESKLDLKSVYDSNEASQDQLIEME 360

Query: 416 NLVESLKESICMAENRAESAEAKLTQLQETNLELTEEVTFLKDSASNKEKKVGSLEKQLR 475
           NL+ESLKESIC++ENRAE+ EAKLTQLQETNLELTEEV+FLKDSASNKEKKVGSLEKQLR
Sbjct: 361 NLIESLKESICISENRAENVEAKLTQLQETNLELTEEVSFLKDSASNKEKKVGSLEKQLR 420

Query: 476 ELEIQLQHAKSSSEASQEQQNMLYSAIWDMETLIEDLKSKVSKAESKTDTAEEHCIILSE 535
           ELEIQLQHAKSSSEASQEQQNMLYSAIWDMETLIE+LKSKVSKAESKTD+AEEHCIILSE
Sbjct: 421 ELEIQLQHAKSSSEASQEQQNMLYSAIWDMETLIEELKSKVSKAESKTDSAEEHCIILSE 480

Query: 536 TNFELNKELTSLKARVEFLEKALDQANSEKFTNANEINLSTKFIMDMVLQLAVERDRIQS 595
           TNFELNKEL SLKAR+EFLEKALDQA S KFTNANEINLSTKFIMDMVLQLAVERDRIQS
Sbjct: 481 TNFELNKELASLKARIEFLEKALDQARSAKFTNANEINLSTKFIMDMVLQLAVERDRIQS 540

Query: 596 QLSILTNDNKSLIERLKNVKDDGSLITRDRGDYDEKERSAPKNDVNILAQ 646
           QLSILTNDNK+LIERLKNVK+  S++TRDRG+YDE++RSAP  DVNILAQ
Sbjct: 541 QLSILTNDNKALIERLKNVKNGASVVTRDRGEYDEEQRSAPMKDVNILAQ 589

BLAST of Spg006966 vs. ExPASy TrEMBL
Match: A0A6J1CZC2 (WPP domain-interacting tail-anchored protein 2-like OS=Momordica charantia OX=3673 GN=LOC111015649 PE=4 SV=1)

HSP 1 Score: 955.7 bits (2469), Expect = 1.0e-274
Identity = 530/591 (89.68%), Postives = 554/591 (93.74%), Query Frame = 0

Query: 59  MDEN----ACNQESERLEMQELENTMKTLTKLELDLAYSSEKLMNLQVLFMCLLAQENDL 118
           MDEN      N+ESERLEMQELENTMKTLTKLELDLAY SEKLMNL VL MCLLAQEND 
Sbjct: 1   MDENPGIYVANEESERLEMQELENTMKTLTKLELDLAYCSEKLMNLHVLLMCLLAQENDF 60

Query: 119 EAMDLESDYIPDDSFGKVLAFDFLSGFLDSEVRELDSFMDTLVAEIVDVRGMISSCRHSS 178
           EAMDLE+DYIPDDSFGKVLAFDFLSGFLDSEVRELDSFM+ L  EI+  R M+SSCR SS
Sbjct: 61  EAMDLENDYIPDDSFGKVLAFDFLSGFLDSEVRELDSFMNMLEEEIIGARRMVSSCRQSS 120

Query: 179 EVFNVVEEKLHDSEKSLIQSRQQILEVKMQSTKLQRIVLSSGNWRLEDPVMSSSQNDQVL 238
           EVFNVVEEKLHDSEKSL+QSR+QILEV MQSTKLQRIVLSSGNWRLEDPV+SS+ NDQVL
Sbjct: 121 EVFNVVEEKLHDSEKSLVQSRKQILEVMMQSTKLQRIVLSSGNWRLEDPVLSSN-NDQVL 180

Query: 239 NINGKSNTMTEQQRHVLRMLEKSLARELDLEKKLSESKQREEELRLKLHYTEQVALRMEE 298
           NI GKSNTMTEQQRH+LRMLEKSLARELDLEKKLSESKQREEELRLKLHYTEQVALRMEE
Sbjct: 181 NIYGKSNTMTEQQRHILRMLEKSLARELDLEKKLSESKQREEELRLKLHYTEQVALRMEE 240

Query: 299 AAEVVWGRFLEADNSVEILTGISKELVGRLQLVQFNLHGSFQRENEIKAKFQDWTEQLKA 358
           AAEVVWGRFLEADNSVE+L GISKELVGRLQLVQFNLHGSFQRENE+K+KFQDWTEQLKA
Sbjct: 241 AAEVVWGRFLEADNSVEVLMGISKELVGRLQLVQFNLHGSFQRENEMKSKFQDWTEQLKA 300

Query: 359 KEVAIQELEKRNAEQIAKNAELDKLREEVKSLEEQLKESRLDLKSAYDSNEASQDQLIEM 418
           KEVAIQELEKRNAE+IAK+AELDKLREEVKSLEEQLKESRLDLK+A++S EASQDQLIEM
Sbjct: 301 KEVAIQELEKRNAEKIAKSAELDKLREEVKSLEEQLKESRLDLKNAFESEEASQDQLIEM 360

Query: 419 ENLVESLKESICMAENRAESAEAKLTQLQETNLELTEEVTFLKDSASNKEKKVGSLEKQL 478
           ENLVESLKE ICMAENRAE AEAKL QLQETNLELTEEV FLKDSAS+KEKKVGSLEKQL
Sbjct: 361 ENLVESLKEGICMAENRAEGAEAKLAQLQETNLELTEEVNFLKDSASSKEKKVGSLEKQL 420

Query: 479 RELEIQLQHAKSSSEASQEQQNMLYSAIWDMETLIEDLKSKVSKAESKTDTAEEHCIILS 538
           RE EIQLQHAKSSSEASQEQQNMLYSAIWDMETLIEDLKSKVSKAESKTDTAEEHCIILS
Sbjct: 421 REQEIQLQHAKSSSEASQEQQNMLYSAIWDMETLIEDLKSKVSKAESKTDTAEEHCIILS 480

Query: 539 ETNFELNKELTSLKARVEFLEKALDQANSEKFTNANEINLSTKFIMDMVLQLAVERDRIQ 598
           E NFELNKELTSLKARVEFLEKALDQANSEK +NANEIN STKFIMDMVLQLAVERDRIQ
Sbjct: 481 EANFELNKELTSLKARVEFLEKALDQANSEKCSNANEINSSTKFIMDMVLQLAVERDRIQ 540

Query: 599 SQLSILTNDNKSLIERLKNVKDDGSLITRDRGDYDEKERSAPKNDVNILAQ 646
            QLSILTNDNK+LIERLKNVKD  S+I  D GDYD+KE+SAPKNDVN LAQ
Sbjct: 541 RQLSILTNDNKALIERLKNVKDGASVIICDGGDYDKKEQSAPKNDVNKLAQ 590

BLAST of Spg006966 vs. ExPASy TrEMBL
Match: A0A1S3CRS0 (WPP domain-interacting tail-anchored protein 2-like OS=Cucumis melo OX=3656 GN=LOC103503596 PE=4 SV=1)

HSP 1 Score: 954.5 bits (2466), Expect = 2.2e-274
Identity = 521/591 (88.16%), Postives = 558/591 (94.42%), Query Frame = 0

Query: 56  VGMMDENACNQESERLEMQELENTMKTLTKLELDLAYSSEKLMNLQVLFMCLLAQENDLE 115
           +GMMDEN CN+ES+RLEMQE+E TMKTLTKLELDLAYSSEKLMNL VL MCLLAQEND E
Sbjct: 1   MGMMDENGCNRESQRLEMQEMEYTMKTLTKLELDLAYSSEKLMNLHVLLMCLLAQENDFE 60

Query: 116 AMDLESDYIPDDSFGKVLAFDFLSGFLDSEVRELDSFMDTLVAEIVDVRGMI-SSCRHSS 175
           AMDLE+DYIPDDSFGK+L FDFLSGFLDSEVRELD+FM+TL AEIVD RGM  SSC  S+
Sbjct: 61  AMDLENDYIPDDSFGKLLVFDFLSGFLDSEVRELDTFMNTLEAEIVDARGMASSSCLQST 120

Query: 176 EVFNVVEEKLHDSEKSLIQSRQQILEVKMQSTKLQRIVLSSGNWRLEDPVMSSSQNDQVL 235
           EVF+V+E KL DSEKSL+QSR+QILEVKMQSTKLQRIVLS GNWRLEDP+M SSQNDQV 
Sbjct: 121 EVFSVLEGKLIDSEKSLVQSRKQILEVKMQSTKLQRIVLSRGNWRLEDPMM-SSQNDQVF 180

Query: 236 NINGKSNTMTEQQRHVLRMLEKSLARELDLEKKLSESKQREEELRLKLHYTEQVALRMEE 295
           NINGKSNTMTEQQRH+LRMLEKSLARELDLEK+LSESKQREEEL++KLHYTEQVALRMEE
Sbjct: 181 NINGKSNTMTEQQRHILRMLEKSLARELDLEKQLSESKQREEELKMKLHYTEQVALRMEE 240

Query: 296 AAEVVWGRFLEADNSVEILTGISKELVGRLQLVQFNLHGSFQRENEIKAKFQDWTEQLKA 355
            AEVVWGRFLEADNSVEIL GISKELVGRLQLVQFNLHGSFQRE+EIK KFQDWTEQL A
Sbjct: 241 TAEVVWGRFLEADNSVEILMGISKELVGRLQLVQFNLHGSFQREHEIKTKFQDWTEQLNA 300

Query: 356 KEVAIQELEKRNAEQIAKNAELDKLREEVKSLEEQLKESRLDLKSAYDSNEASQDQLIEM 415
           KEVAIQ+LEKRNAE IAKNAELDKLREEVKSLEEQLKE RLDLK+AY+SNEASQDQLIEM
Sbjct: 301 KEVAIQKLEKRNAELIAKNAELDKLREEVKSLEEQLKELRLDLKTAYESNEASQDQLIEM 360

Query: 416 ENLVESLKESICMAENRAESAEAKLTQLQETNLELTEEVTFLKDSASNKEKKVGSLEKQL 475
           ENLVESLKESIC++ENRAES E KLTQLQETNLELTEEV+FLKDSAS+KEKKVGSLEKQL
Sbjct: 361 ENLVESLKESICISENRAESVETKLTQLQETNLELTEEVSFLKDSASSKEKKVGSLEKQL 420

Query: 476 RELEIQLQHAKSSSEASQEQQNMLYSAIWDMETLIEDLKSKVSKAESKTDTAEEHCIILS 535
           RELEIQLQHAKSSSEASQEQQNMLYSAIWDMETLIEDLKSKVSKAESKTD+AEEHCIILS
Sbjct: 421 RELEIQLQHAKSSSEASQEQQNMLYSAIWDMETLIEDLKSKVSKAESKTDSAEEHCIILS 480

Query: 536 ETNFELNKELTSLKARVEFLEKALDQANSEKFTNANEINLSTKFIMDMVLQLAVERDRIQ 595
           ETNFELNKELTSLK RVEFLEKALDQAN EK+ NANEINLS+KFIMDMVLQLAVERDR+Q
Sbjct: 481 ETNFELNKELTSLKGRVEFLEKALDQANGEKYANANEINLSSKFIMDMVLQLAVERDRVQ 540

Query: 596 SQLSILTNDNKSLIERLKNVKDDGSLITRDRGDYDEKERSAPKNDVNILAQ 646
            QLSILTNDNK+LIE+LKN++D  S++TRDR DYDE+ERSAPK DVNILAQ
Sbjct: 541 RQLSILTNDNKALIEKLKNIRDGASIVTRDREDYDEEERSAPKKDVNILAQ 590

BLAST of Spg006966 vs. ExPASy TrEMBL
Match: A0A0A0KJA1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G136890 PE=4 SV=1)

HSP 1 Score: 954.1 bits (2465), Expect = 2.9e-274
Identity = 519/590 (87.97%), Postives = 555/590 (94.07%), Query Frame = 0

Query: 56  VGMMDENACNQESERLEMQELENTMKTLTKLELDLAYSSEKLMNLQVLFMCLLAQENDLE 115
           +GMMDEN C++ES+RLEMQE+E TMKTLTKLELDLAYSSEKLMNL VL MCLLAQEND E
Sbjct: 1   MGMMDENVCHRESQRLEMQEMEYTMKTLTKLELDLAYSSEKLMNLHVLLMCLLAQENDFE 60

Query: 116 AMDLESDYIPDDSFGKVLAFDFLSGFLDSEVRELDSFMDTLVAEIVDVRGMISSCRHSSE 175
           AMDL  DYIPDDSFGKVL FDFLSGFLDSEVRELDSFM+TL AE VD RGM+S+C  S+E
Sbjct: 61  AMDLVHDYIPDDSFGKVLVFDFLSGFLDSEVRELDSFMNTLEAETVDARGMVSTCLQSTE 120

Query: 176 VFNVVEEKLHDSEKSLIQSRQQILEVKMQSTKLQRIVLSSGNWRLEDPVMSSSQNDQVLN 235
           VF+V+E KL DSEKSL+QSR+QILEVKMQSTKLQRIVLSSGNWRLEDP+M SSQNDQV N
Sbjct: 121 VFSVLEGKLLDSEKSLVQSRKQILEVKMQSTKLQRIVLSSGNWRLEDPMM-SSQNDQVFN 180

Query: 236 INGKSNTMTEQQRHVLRMLEKSLARELDLEKKLSESKQREEELRLKLHYTEQVALRMEEA 295
           INGKSNTMTEQQRH+LRMLEKSLARELDLEK+LSESKQREEEL++KLHYTEQVALRMEE 
Sbjct: 181 INGKSNTMTEQQRHILRMLEKSLARELDLEKQLSESKQREEELKMKLHYTEQVALRMEET 240

Query: 296 AEVVWGRFLEADNSVEILTGISKELVGRLQLVQFNLHGSFQRENEIKAKFQDWTEQLKAK 355
           AEVVWGRFLEADNSVEIL GISKE VGRLQLVQFNLHGSFQREN+IKAKFQDWTEQL AK
Sbjct: 241 AEVVWGRFLEADNSVEILMGISKEFVGRLQLVQFNLHGSFQRENDIKAKFQDWTEQLNAK 300

Query: 356 EVAIQELEKRNAEQIAKNAELDKLREEVKSLEEQLKESRLDLKSAYDSNEASQDQLIEME 415
           EVAIQ+LEKRNAE IAKNAELDKLREEVKSLEEQLKESRLDLKSAYDSNEASQDQLIEME
Sbjct: 301 EVAIQKLEKRNAELIAKNAELDKLREEVKSLEEQLKESRLDLKSAYDSNEASQDQLIEME 360

Query: 416 NLVESLKESICMAENRAESAEAKLTQLQETNLELTEEVTFLKDSASNKEKKVGSLEKQLR 475
           NLVE+LKESIC++ENRAE AE KLTQLQETNLELTEEV+FLKDS SNKEKKVGSLEKQLR
Sbjct: 361 NLVETLKESICISENRAEGAETKLTQLQETNLELTEEVSFLKDSVSNKEKKVGSLEKQLR 420

Query: 476 ELEIQLQHAKSSSEASQEQQNMLYSAIWDMETLIEDLKSKVSKAESKTDTAEEHCIILSE 535
           ELEIQLQHAKSSSEASQEQQNMLYSAIWDMETLIEDLKSKVSKAESKTD+AEEHCIILSE
Sbjct: 421 ELEIQLQHAKSSSEASQEQQNMLYSAIWDMETLIEDLKSKVSKAESKTDSAEEHCIILSE 480

Query: 536 TNFELNKELTSLKARVEFLEKALDQANSEKFTNANEINLSTKFIMDMVLQLAVERDRIQS 595
           TNFELNKELTSLK +VEFLEK+LDQAN EK+ NANEINLS+KF+MDMVLQLAVERDRIQS
Sbjct: 481 TNFELNKELTSLKGQVEFLEKSLDQANGEKYANANEINLSSKFVMDMVLQLAVERDRIQS 540

Query: 596 QLSILTNDNKSLIERLKNVKDDGSLITRDRGDYDEKERSAPKNDVNILAQ 646
           QLSILTNDNK+LIE+LKNV+D   ++T  R DYDEKE+SAPK DVNILAQ
Sbjct: 541 QLSILTNDNKALIEKLKNVRDSAPIVTLHREDYDEKEQSAPKKDVNILAQ 589

BLAST of Spg006966 vs. ExPASy TrEMBL
Match: A0A6J1FMT1 (WPP domain-interacting tail-anchored protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC111445366 PE=4 SV=1)

HSP 1 Score: 952.6 bits (2461), Expect = 8.5e-274
Identity = 522/590 (88.47%), Postives = 558/590 (94.58%), Query Frame = 0

Query: 56  VGMMDENACNQESERLEMQELENTMKTLTKLELDLAYSSEKLMNLQVLFMCLLAQENDLE 115
           +GMMDENACNQES+ LEMQELE+TMKTLTKLEL+LAYSSEKL NL VL MCL+AQEND E
Sbjct: 1   MGMMDENACNQESQGLEMQELESTMKTLTKLELNLAYSSEKLTNLHVLLMCLMAQENDFE 60

Query: 116 AMDLESDYIPDDSFGKVLAFDFLSGFLDSEVRELDSFMDTLVAEIVDVRGMISSCRHSSE 175
            +DLE+D IPDD F KVLAFDFLSGFLDSEVRELDS MD+L AEIVD RGM SSCR SSE
Sbjct: 61  GVDLENDSIPDDLFEKVLAFDFLSGFLDSEVRELDSNMDSLEAEIVDSRGMTSSCRQSSE 120

Query: 176 VFNVVEEKLHDSEKSLIQSRQQILEVKMQSTKLQRIVLSSGNWRLEDPVMSSSQNDQVLN 235
           VFNVVEEKL+DSE+S++QSRQQILEVKMQ  KLQR+VLSSGNWRLEDPVM S QNDQVLN
Sbjct: 121 VFNVVEEKLNDSERSIVQSRQQILEVKMQLAKLQRMVLSSGNWRLEDPVM-SYQNDQVLN 180

Query: 236 INGKSNTMTEQQRHVLRMLEKSLARELDLEKKLSESKQREEELRLKLHYTEQVALRMEEA 295
           INGKSN +TEQQRH+LRMLEKSLARELDLEKKLSESKQREEELRLKLHYTEQV LRMEEA
Sbjct: 181 INGKSNPITEQQRHILRMLEKSLARELDLEKKLSESKQREEELRLKLHYTEQVTLRMEEA 240

Query: 296 AEVVWGRFLEADNSVEILTGISKELVGRLQLVQFNLHGSFQRENEIKAKFQDWTEQLKAK 355
           AEVVWGRFLEADNSVEIL GISKELVGRLQLVQFNLHGSFQRENEIK KFQDWTEQLKAK
Sbjct: 241 AEVVWGRFLEADNSVEILMGISKELVGRLQLVQFNLHGSFQRENEIKDKFQDWTEQLKAK 300

Query: 356 EVAIQELEKRNAEQIAKNAELDKLREEVKSLEEQLKESRLDLKSAYDSNEASQDQLIEME 415
           EV+IQ+LEKRNA+ IAKNAELDKLREEVKSLE+Q+KES+LDLKSAYDSNEASQDQLIEME
Sbjct: 301 EVSIQKLEKRNADLIAKNAELDKLREEVKSLEDQVKESKLDLKSAYDSNEASQDQLIEME 360

Query: 416 NLVESLKESICMAENRAESAEAKLTQLQETNLELTEEVTFLKDSASNKEKKVGSLEKQLR 475
           NL+ESLKESIC++ENRAE+ EAKLTQLQETNLELTEEV+FLKDSASNKEKKVGSLEKQLR
Sbjct: 361 NLIESLKESICISENRAENVEAKLTQLQETNLELTEEVSFLKDSASNKEKKVGSLEKQLR 420

Query: 476 ELEIQLQHAKSSSEASQEQQNMLYSAIWDMETLIEDLKSKVSKAESKTDTAEEHCIILSE 535
           ELEIQLQHAKSSSEASQEQQNMLYSAIWDMETLIE+LKSKVSKAESKTD+AEEH IILSE
Sbjct: 421 ELEIQLQHAKSSSEASQEQQNMLYSAIWDMETLIEELKSKVSKAESKTDSAEEHYIILSE 480

Query: 536 TNFELNKELTSLKARVEFLEKALDQANSEKFTNANEINLSTKFIMDMVLQLAVERDRIQS 595
           TNFELNKEL SLKAR+EFLEKALDQA S KFTNANEINLSTKFIMDMVLQLAVERDRIQS
Sbjct: 481 TNFELNKELASLKARIEFLEKALDQARSAKFTNANEINLSTKFIMDMVLQLAVERDRIQS 540

Query: 596 QLSILTNDNKSLIERLKNVKDDGSLITRDRGDYDEKERSAPKNDVNILAQ 646
           QLS+LTNDNK+LIERLKNVK+  S++TRDRGDYDE++RSAP  DVNILAQ
Sbjct: 541 QLSLLTNDNKALIERLKNVKNGASVVTRDRGDYDEEQRSAPMKDVNILAQ 589

BLAST of Spg006966 vs. TAIR 10
Match: AT1G68910.1 (WPP domain-interacting protein 2 )

HSP 1 Score: 474.9 bits (1221), Expect = 1.0e-133
Identity = 293/537 (54.56%), Postives = 384/537 (71.51%), Query Frame = 0

Query: 74  QELENTMKTLTKLELDLAYSSEKLMNLQVLFMCLLAQENDLEAM-DLESDYIPDDSFGKV 133
           +E +  ++ LTKLE+D AY+SEKLMNL VL M LLA +NDLE M  L+S      SF K 
Sbjct: 18  EEEQEMLRMLTKLEIDSAYTSEKLMNLHVLLMHLLAWDNDLEGMGTLDSS---PASFEKA 77

Query: 134 LAFDFLSGFLDSEVRELDSFMDTLVAEIVDVRGMISSCRHSSEVFNVVEEKLHDSEKSLI 193
           L FD L G L+SEV+E+D  +D L A+IVD    ISSC+H + +  V+E KL +S +SL 
Sbjct: 78  LTFDLLCGILESEVKEVDEVLDVLEAQIVDTSYKISSCKHGNYI--VIEGKLGESAESLK 137

Query: 194 QSRQQILEVKMQSTKLQRIVLSSGNWRLEDPVMSSSQNDQVLNINGKSNTMTEQQRH--V 253
           QSR Q+ E+ +Q  +L+R +    N         +S+N++ + +  K        RH   
Sbjct: 138 QSRGQVSEITLQLAQLRRTLHYIRN--------GTSENEESVELRQKYALKPSDLRHKNA 197

Query: 254 LRMLEKSLARELDLEKKLSESKQREEELRLKLHYTEQVALRMEEAAEVVWGRFLEADNSV 313
           LRMLEKSL+REL+LEKKL E +Q EE+L+LKLHYTE+V+ RMEEA+E +WGRFLEADNS 
Sbjct: 198 LRMLEKSLSRELELEKKLMEFQQNEEQLKLKLHYTEEVSSRMEEASEFIWGRFLEADNSS 257

Query: 314 EILTGISKELVGRLQLVQFNLHGSFQRENEIKAKFQDWTEQLKAKEVAIQELEKRNAEQI 373
           E+LTGISKELVGRLQ++QF+L+GS QRE+E+K+K +D T QL+AK++ +Q+LE   +E  
Sbjct: 258 EVLTGISKELVGRLQILQFSLNGSAQRESELKSKLEDCTVQLEAKDLLVQKLEGTISENS 317

Query: 374 AKNAELDKLREEVKSLEEQLKESRLDLKSAYDSNEASQDQLIEMENLVESLKESICMAEN 433
              +E+  LRE VKS E++LK + L+LKS   S +     L EMEN  ES+KE++  AE+
Sbjct: 318 EIVSEVLTLREYVKSAEQKLKNTDLELKSVNASKQEILVHLAEMENANESVKENLFEAES 377

Query: 434 RAESAEAKLTQLQETNLELTEEVTFLKDSASNKEKKVGSLEKQLRELEIQLQHAKSSSEA 493
           RAES EAK+ +L   NLELTEE+ FLKD+   K KKV SLEKQ+RELE+Q+Q++K SSEA
Sbjct: 378 RAESGEAKIKELDAANLELTEELNFLKDADDKKTKKVNSLEKQVRELEVQVQNSKVSSEA 437

Query: 494 SQEQQNMLYSAIWDMETLIEDLKSKVSKAESKTDTAEEHCIILSETNFELNKELTSLKAR 553
           +QEQQNMLYSAIWDMETLIEDLKSK SKAES+T+T EE CI+LS TN ELNK+++ L+ +
Sbjct: 438 NQEQQNMLYSAIWDMETLIEDLKSKASKAESRTETVEEQCIVLSTTNSELNKDVSFLRQK 497

Query: 554 VEFLEKALDQANSEKFTNANEINLSTKFIMDMVLQLAVERDRIQSQLSILTNDNKSL 608
            + LE  LD AN+EK   A EI    K +MDM+LQL+ ER+RIQ QL  L  +NK L
Sbjct: 498 AKSLEAMLDLANNEKERYAQEITTRNKVLMDMMLQLSSERERIQEQLYSLAKENKIL 541

BLAST of Spg006966 vs. TAIR 10
Match: AT1G68910.2 (WPP domain-interacting protein 2 )

HSP 1 Score: 474.9 bits (1221), Expect = 1.0e-133
Identity = 293/537 (54.56%), Postives = 384/537 (71.51%), Query Frame = 0

Query: 74  QELENTMKTLTKLELDLAYSSEKLMNLQVLFMCLLAQENDLEAM-DLESDYIPDDSFGKV 133
           +E +  ++ LTKLE+D AY+SEKLMNL VL M LLA +NDLE M  L+S      SF K 
Sbjct: 18  EEEQEMLRMLTKLEIDSAYTSEKLMNLHVLLMHLLAWDNDLEGMGTLDSS---PASFEKA 77

Query: 134 LAFDFLSGFLDSEVRELDSFMDTLVAEIVDVRGMISSCRHSSEVFNVVEEKLHDSEKSLI 193
           L FD L G L+SEV+E+D  +D L A+IVD    ISSC+H + +  V+E KL +S +SL 
Sbjct: 78  LTFDLLCGILESEVKEVDEVLDVLEAQIVDTSYKISSCKHGNYI--VIEGKLGESAESLK 137

Query: 194 QSRQQILEVKMQSTKLQRIVLSSGNWRLEDPVMSSSQNDQVLNINGKSNTMTEQQRH--V 253
           QSR Q+ E+ +Q  +L+R +    N         +S+N++ + +  K        RH   
Sbjct: 138 QSRGQVSEITLQLAQLRRTLHYIRN--------GTSENEESVELRQKYALKPSDLRHKNA 197

Query: 254 LRMLEKSLARELDLEKKLSESKQREEELRLKLHYTEQVALRMEEAAEVVWGRFLEADNSV 313
           LRMLEKSL+REL+LEKKL E +Q EE+L+LKLHYTE+V+ RMEEA+E +WGRFLEADNS 
Sbjct: 198 LRMLEKSLSRELELEKKLMEFQQNEEQLKLKLHYTEEVSSRMEEASEFIWGRFLEADNSS 257

Query: 314 EILTGISKELVGRLQLVQFNLHGSFQRENEIKAKFQDWTEQLKAKEVAIQELEKRNAEQI 373
           E+LTGISKELVGRLQ++QF+L+GS QRE+E+K+K +D T QL+AK++ +Q+LE   +E  
Sbjct: 258 EVLTGISKELVGRLQILQFSLNGSAQRESELKSKLEDCTVQLEAKDLLVQKLEGTISENS 317

Query: 374 AKNAELDKLREEVKSLEEQLKESRLDLKSAYDSNEASQDQLIEMENLVESLKESICMAEN 433
              +E+  LRE VKS E++LK + L+LKS   S +     L EMEN  ES+KE++  AE+
Sbjct: 318 EIVSEVLTLREYVKSAEQKLKNTDLELKSVNASKQEILVHLAEMENANESVKENLFEAES 377

Query: 434 RAESAEAKLTQLQETNLELTEEVTFLKDSASNKEKKVGSLEKQLRELEIQLQHAKSSSEA 493
           RAES EAK+ +L   NLELTEE+ FLKD+   K KKV SLEKQ+RELE+Q+Q++K SSEA
Sbjct: 378 RAESGEAKIKELDAANLELTEELNFLKDADDKKTKKVNSLEKQVRELEVQVQNSKVSSEA 437

Query: 494 SQEQQNMLYSAIWDMETLIEDLKSKVSKAESKTDTAEEHCIILSETNFELNKELTSLKAR 553
           +QEQQNMLYSAIWDMETLIEDLKSK SKAES+T+T EE CI+LS TN ELNK+++ L+ +
Sbjct: 438 NQEQQNMLYSAIWDMETLIEDLKSKASKAESRTETVEEQCIVLSTTNSELNKDVSFLRQK 497

Query: 554 VEFLEKALDQANSEKFTNANEINLSTKFIMDMVLQLAVERDRIQSQLSILTNDNKSL 608
            + LE  LD AN+EK   A EI    K +MDM+LQL+ ER+RIQ QL  L  +NK L
Sbjct: 498 AKSLEAMLDLANNEKERYAQEITTRNKVLMDMMLQLSSERERIQEQLYSLAKENKIL 541

BLAST of Spg006966 vs. TAIR 10
Match: AT1G68910.3 (WPP domain-interacting protein 2 )

HSP 1 Score: 474.9 bits (1221), Expect = 1.0e-133
Identity = 293/537 (54.56%), Postives = 384/537 (71.51%), Query Frame = 0

Query: 74  QELENTMKTLTKLELDLAYSSEKLMNLQVLFMCLLAQENDLEAM-DLESDYIPDDSFGKV 133
           +E +  ++ LTKLE+D AY+SEKLMNL VL M LLA +NDLE M  L+S      SF K 
Sbjct: 18  EEEQEMLRMLTKLEIDSAYTSEKLMNLHVLLMHLLAWDNDLEGMGTLDSS---PASFEKA 77

Query: 134 LAFDFLSGFLDSEVRELDSFMDTLVAEIVDVRGMISSCRHSSEVFNVVEEKLHDSEKSLI 193
           L FD L G L+SEV+E+D  +D L A+IVD    ISSC+H + +  V+E KL +S +SL 
Sbjct: 78  LTFDLLCGILESEVKEVDEVLDVLEAQIVDTSYKISSCKHGNYI--VIEGKLGESAESLK 137

Query: 194 QSRQQILEVKMQSTKLQRIVLSSGNWRLEDPVMSSSQNDQVLNINGKSNTMTEQQRH--V 253
           QSR Q+ E+ +Q  +L+R +    N         +S+N++ + +  K        RH   
Sbjct: 138 QSRGQVSEITLQLAQLRRTLHYIRN--------GTSENEESVELRQKYALKPSDLRHKNA 197

Query: 254 LRMLEKSLARELDLEKKLSESKQREEELRLKLHYTEQVALRMEEAAEVVWGRFLEADNSV 313
           LRMLEKSL+REL+LEKKL E +Q EE+L+LKLHYTE+V+ RMEEA+E +WGRFLEADNS 
Sbjct: 198 LRMLEKSLSRELELEKKLMEFQQNEEQLKLKLHYTEEVSSRMEEASEFIWGRFLEADNSS 257

Query: 314 EILTGISKELVGRLQLVQFNLHGSFQRENEIKAKFQDWTEQLKAKEVAIQELEKRNAEQI 373
           E+LTGISKELVGRLQ++QF+L+GS QRE+E+K+K +D T QL+AK++ +Q+LE   +E  
Sbjct: 258 EVLTGISKELVGRLQILQFSLNGSAQRESELKSKLEDCTVQLEAKDLLVQKLEGTISENS 317

Query: 374 AKNAELDKLREEVKSLEEQLKESRLDLKSAYDSNEASQDQLIEMENLVESLKESICMAEN 433
              +E+  LRE VKS E++LK + L+LKS   S +     L EMEN  ES+KE++  AE+
Sbjct: 318 EIVSEVLTLREYVKSAEQKLKNTDLELKSVNASKQEILVHLAEMENANESVKENLFEAES 377

Query: 434 RAESAEAKLTQLQETNLELTEEVTFLKDSASNKEKKVGSLEKQLRELEIQLQHAKSSSEA 493
           RAES EAK+ +L   NLELTEE+ FLKD+   K KKV SLEKQ+RELE+Q+Q++K SSEA
Sbjct: 378 RAESGEAKIKELDAANLELTEELNFLKDADDKKTKKVNSLEKQVRELEVQVQNSKVSSEA 437

Query: 494 SQEQQNMLYSAIWDMETLIEDLKSKVSKAESKTDTAEEHCIILSETNFELNKELTSLKAR 553
           +QEQQNMLYSAIWDMETLIEDLKSK SKAES+T+T EE CI+LS TN ELNK+++ L+ +
Sbjct: 438 NQEQQNMLYSAIWDMETLIEDLKSKASKAESRTETVEEQCIVLSTTNSELNKDVSFLRQK 497

Query: 554 VEFLEKALDQANSEKFTNANEINLSTKFIMDMVLQLAVERDRIQSQLSILTNDNKSL 608
            + LE  LD AN+EK   A EI    K +MDM+LQL+ ER+RIQ QL  L  +NK L
Sbjct: 498 AKSLEAMLDLANNEKERYAQEITTRNKVLMDMMLQLSSERERIQEQLYSLAKENKIL 541

BLAST of Spg006966 vs. TAIR 10
Match: AT5G11390.1 (WPP domain-interacting protein 1 )

HSP 1 Score: 348.6 bits (893), Expect = 1.1e-95
Identity = 250/604 (41.39%), Postives = 365/604 (60.43%), Query Frame = 0

Query: 75  ELENTMKTLTKLELDLAYSSEKLMNLQVLFMCLLAQENDLEAM---DLESDYIP------ 134
           +L +  + LTK+ELD A+ SEKL+NL +L M L  +END E+      E +  P      
Sbjct: 44  QLSSAGEVLTKVELDFAFVSEKLVNLSLLTMQLGTRENDFESFVSKKEEDEEEPSSNVDD 103

Query: 135 -DDSFGKVLAFDFLSGFLDSEVRELDSFMDTLVAEIVDVRGMISSCRHSSEVFNVVEEKL 194
            DDS  K L FD LS  L+SEV+EL+S +  L  EI   R MIS  +H  E F  +E KL
Sbjct: 104 DDDSAEKALEFDLLSSILNSEVKELESLLGFLQNEIQSARVMISPFQHDGEAFLDLEGKL 163

Query: 195 HDSEKSLIQSRQQILEVKMQSTKLQRI---VLSSGNWRLEDPVMSSSQND-QVLNINGKS 254
           +D+E+SL Q  +Q++E+K QS+  QR+   +   G+W       S SQND +  +++ K 
Sbjct: 164 NDTEQSLGQLMEQVVEMKKQSSNFQRLSSGLDEQGSW--SGGQTSVSQNDGEFGDLSAKI 223

Query: 255 NTMT-EQQRHVLRMLEKSLARELDLEKKLSESKQREEELRLKLHYTEQVALRMEEAAEVV 314
           N  T +QQR+VLRMLEKSLA+E++LEKKLSES+  E EL +KL+ +EQ  + MEE  E  
Sbjct: 224 NMQTADQQRNVLRMLEKSLAKEMELEKKLSESRNTERELEMKLYSSEQDVVYMEEVTEDA 283

Query: 315 WGRFLEADNSVEILTGISKELVGRLQLVQFNLHGSFQRENEIKAKFQDWTEQLKAKEVAI 374
           + R+LEADN+ E+  G SKE+ G+LQ++QFNL GSF+RE+ +K+K  D  E+L+AKE A+
Sbjct: 284 FSRWLEADNAAEVFKGTSKEMSGKLQILQFNLSGSFKREDNLKSKLVDSKERLEAKECAL 343

Query: 375 QELEKRNA----------------------EQIAKNAELDKLREEVKSLEEQLKESRLDL 434
            +L+  NA                      + I  N E   L E+V SLEEQL E  +  
Sbjct: 344 HKLDSSNARLADFLVAQTEGLKESLQEAEEKLILLNTENSTLSEKVSSLEEQLNEYGIQT 403

Query: 435 KSAYDSNEASQDQLIEMENLVESLKESICMAENRAESAEAKLTQLQETNLELTEEVTFLK 494
           +   D++  S   + ++E + E LK+ +   E RAE  E+K   L+E+  EL +E+   +
Sbjct: 404 E---DADATSGALITDLERINEELKDKLAKTEARAEETESKCKILEESKKELQDELGNFR 463

Query: 495 DSASNKEKKVGSLEKQLRELEIQLQHAKSSSEASQEQQNMLYSAIWDMETLIEDLKSKVS 554
           D       K+ SLEK LR+ ++QL+HA ++ EAS+E+QN+LYS + DME +IEDLKSKV 
Sbjct: 464 DKGFTIH-KLASLEKHLRDSDLQLEHAVAAVEASKEKQNLLYSTVSDMEDVIEDLKSKVL 523

Query: 555 KAESKTDTAEEHCIILSETNFELNKELTSLKARVEFLEKALDQANSEKFTNANEINLSTK 614
           KAE++ D  EE  I++SE+N E+N+EL   K R++  EK L QA   K   A +I +  K
Sbjct: 524 KAENRADITEEKLIMVSESNAEVNEELKFFKGRLKEGEKYLQQAEERKLRTAKDIGVHNK 583

Query: 615 FIMDMVLQLAVERDRIQSQLSILTNDNKSLIERLKNVKDDGSLITRDRGDYDEKERSAPK 642
            +  +V+QLA ER+R+  Q++ L+ +N  L+ +LK V   G +   + G+  E    +PK
Sbjct: 584 IMKKLVMQLAAERERLHKQITNLSRENCVLMVKLKKVGKTGYM---ESGNGSE---VSPK 635

BLAST of Spg006966 vs. TAIR 10
Match: AT2G32240.1 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: response to cadmium ion; LOCATED IN: plasma membrane; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Prefoldin (InterPro:IPR009053); BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G05320.3); Has 470429 Blast hits to 168274 proteins in 4282 species: Archae - 6896; Bacteria - 131956; Metazoa - 175525; Fungi - 33166; Plants - 25441; Viruses - 2243; Other Eukaryotes - 95202 (source: NCBI BLink). )

HSP 1 Score: 59.7 bits (143), Expect = 1.0e-08
Identity = 124/614 (20.20%), Postives = 248/614 (40.39%), Query Frame = 0

Query: 54   QAVGMMDENACNQESERLEMQELENTMKTLTKLELDLAYSSEKLMNLQVLFMCLLAQEND 113
            Q + ++   + + E E  E+ E  + ++T     +++A   +K    Q+       QE  
Sbjct: 515  QQLNLLQLKSSDAERELKELSEKSSELQT----AIEVAEEEKKQATTQM-------QEYK 574

Query: 114  LEAMDLESDYIPDDSFGKVLAFDFLSGFLDSEVRELDSFMDTLVAEIVDVRGMISSCRHS 173
             +A +LE       +    L  D        +  E +   +T     +++ G+   C+ S
Sbjct: 575  QKASELELSLTQSSARNSELEEDLRIAL--QKGAEHEDRANTTHQRSIELEGL---CQSS 634

Query: 174  SEVFNVVEEKLHDSEKSLIQSRQQILEVKMQSTKLQRIVLSSGNWRLEDPVMSSSQNDQV 233
                   E +L D E  L   + +I E++ Q + L++                    +  
Sbjct: 635  QSKHEDAEGRLKDLELLLQTEKYRIQELEEQVSSLEK-----------------KHGETE 694

Query: 234  LNINGKSNTMTEQQRHVLRMLEKSLARELDLEKKLSESKQREEELRLKLHYTEQVALRME 293
             +  G    + E Q      LE    +   LE  L+ + + E+EL   L+       ++E
Sbjct: 695  ADSKGYLGQVAELQ----STLEAFQVKSSSLEAALNIATENEKELTENLNAVTSEKKKLE 754

Query: 294  EAAEVVWGRFLEADNSVEILTGISKELVGRLQLVQFNLHGSFQRENEIKAKFQDWTEQLK 353
               +    +  E++N +E +        G+L+ ++ +L  +  +E+E+  K +   E L+
Sbjct: 755  ATVDEYSVKISESENLLESIRNELNVTQGKLESIENDLKAAGLQESEVMEKLKSAEESLE 814

Query: 354  AK-----------------------------EVAIQELEKRNAEQIAKNAELDKLREEVK 413
             K                             + A++E   R++E  +   +L  L  ++K
Sbjct: 815  QKGREIDEATTKRMELEALHQSLSIDSEHRLQKAMEEFTSRDSEASSLTEKLRDLEGKIK 874

Query: 414  SLEEQLKESRLDLKSAYDSNEASQDQLIEMENLVESLKESICMAENRAESAEAKLTQLQE 473
            S EEQL E+     S  +  E +  +L   E++ E LK+    A+ ++  + ++   L E
Sbjct: 875  SYEEQLAEASGKSSSLKEKLEQTLGRLAAAESVNEKLKQEFDQAQEKSLQSSSESELLAE 934

Query: 474  TNLEL---------------TEEVTFLK------DSASNKEKKVGSLEKQLRELEIQLQH 533
            TN +L                E+ T LK      +  + KE +   L ++L+  E Q++ 
Sbjct: 935  TNNQLKIKIQELEGLIGSGSVEKETALKRLEEAIERFNQKETESSDLVEKLKTHENQIEE 994

Query: 534  AKSSSEASQEQQNMLYSAIWDMETLIEDLKSKVSKAESKTDTAEEHCIILSETNFELNKE 593
             K  +  +    +     + D  + +++L+S + +  +K    E+    L+E N +LN E
Sbjct: 995  YKKLAHEASGVADTRKVELEDALSKLKNLESTIEELGAKCQGLEKESGDLAEVNLKLNLE 1054

Query: 594  LTSLKARVEFLEKALDQANSEKFTNANEINLSTKFIMDMVLQLAVERDRIQSQLSILTND 618
            L +  +    L+  L    +EK   ANE+  S   I D+  QL  E +++QSQ+S  T +
Sbjct: 1055 LANHGSEANELQTKLSALEAEKEQTANELEASKTTIEDLTKQLTSEGEKLQSQISSHTEE 1091