Spg006896 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg006896 vs. NCBI nr
Match: XP_038874970.1 (uncharacterized protein LOC120067490 [Benincasa hispida])

HSP 1 Score: 426.0 bits (1094), Expect = 2.8e-115
Identity = 211/304 (69.41%), Postives = 254/304 (83.55%), Query Frame = 0

Query: 17  VVRSSRDQKPLHYTLQIQSFSLLKAAL-ASSERDRYESQTFNAGGYKWKLALYPNGDEKR 76
           V+R+SRD+KPLHYTL+IQSFSLLKAA+ ASS R+RYESQ F AGGYKWKLALYPNGD KR
Sbjct: 26  VIRTSRDEKPLHYTLKIQSFSLLKAAVAASSIRNRYESQIFKAGGYKWKLALYPNGDFKR 85

Query: 77  NGSDHISLYLVMAEDDVLWTTTSEVNVVFTFLVYNHLKDKYLIVQDGKVRRFHAIRKEWG 136
           N ++HISLYLV AED++  ++  E+ V+  FLVY++LKD+Y  +QDGKVR FHAI+KEWG
Sbjct: 86  NVANHISLYLVSAEDEIS-SSGLEIKVMIKFLVYDNLKDRYWTIQDGKVRCFHAIKKEWG 145

Query: 137 FEKLVSLDTFNDVSNGLLVDDCCVFGVDVIVMKC-DGNGEVISLIKQPKNYKYTWKFTNF 196
           FEKLVSLDTFND SNGLLVDDCC FGVDVIVMK  DGN E++SLIKQ KNYKYTWK  NF
Sbjct: 146 FEKLVSLDTFNDTSNGLLVDDCCAFGVDVIVMKSYDGNNEILSLIKQLKNYKYTWKINNF 205

Query: 197 SQKKLNLYESDPFTVENYCWKIRLYPRGDSKSKTGYLSLYLMFDSYKELPQGFQVFVEWE 256
           SQ   N+YESDPFTV+NY WKI LYPRGDS + TGY+S++L FDS+++LPQG +V+VE++
Sbjct: 206 SQLSKNVYESDPFTVDNYTWKIELYPRGDSLAMTGYMSMFLRFDSFEKLPQGSRVYVEYD 265

Query: 257 MSVLSQFGVGHRTFTPKLWFEGE-EGRGWPNFMSLSNLKEPKKGFLRDDTLIVEVKINVI 316
           ++VLSQ G  +  +  K WF+GE +  G+ NFMSLSN+KEP KGFL DDTL+VEVKINVI
Sbjct: 266 IAVLSQLGGAYTNYPVKHWFDGETKSWGFGNFMSLSNVKEPMKGFLCDDTLMVEVKINVI 325

Query: 317 STSK 318
           S  K
Sbjct: 326 SILK 328

BLAST of Spg006896 vs. NCBI nr
Match: XP_038875772.1 (uncharacterized protein LOC120068142 [Benincasa hispida])

HSP 1 Score: 409.1 bits (1050), Expect = 3.6e-110
Identity = 208/310 (67.10%), Postives = 249/310 (80.32%), Query Frame = 0

Query: 16  AVVRSSRDQKPLHYTLQIQSFSLLKAALASSERDRYESQTFNAGGYKWKLALYPNGDEKR 75
           +VV S+R +KP+HYTL+IQSFSLLK  LASS+R R+ESQ FNAG Y+WKLALYPNGDE+R
Sbjct: 24  SVVMSTRHEKPVHYTLKIQSFSLLKNGLASSQRQRFESQKFNAGCYEWKLALYPNGDERR 83

Query: 76  NGSDHISLYLVMAEDDVLWTTTSEVNVVFTFLVYNHLKDKYLIVQDGKVRRFHAIRKEWG 135
           N S+HISLYLV+  +++L +TTSEVNVVFTFLVY+ L+ KYL VQDGK+RRF A++ EWG
Sbjct: 84  NVSNHISLYLVIVGNNIL-STTSEVNVVFTFLVYDTLRGKYLTVQDGKMRRFSAMKTEWG 143

Query: 136 FEKLVSLDTFNDVSNGLLVDDCCVFGVDVIVMKCDGN-GEVISLIKQPKNYKYTWKFTNF 195
            EKL+ LDTFND SNG LVDDCCVFGVD+ VM CDG  GEV SLIKQPKNY+YTWK  NF
Sbjct: 144 IEKLLPLDTFNDASNGFLVDDCCVFGVDIFVMSCDGGMGEVFSLIKQPKNYEYTWKLNNF 203

Query: 196 SQKKLNLYESDPFTVENYCWKIRLYPRGDSKSKTGYLSLYLMFDSYKELPQGFQVFVEWE 255
           SQ    L ES+PFTVE+Y WKI LYP G SK+K+G+LS+YL+FDS  +LPQG QV+VE+E
Sbjct: 204 SQLGSTLKESNPFTVESYRWKILLYPSGCSKAKSGFLSMYLLFDSSNQLPQGSQVYVEYE 263

Query: 256 MSVLSQFGVGHRTFTPKLW--FEG-EEGRGW--PNFMSLSNLKEPKKGFLRDDTLIVEVK 315
           M++LSQ        T   W  F+G E GRGW  PNF SLS+L+EP KG+L +DTL+ EVK
Sbjct: 264 MAILSQLEAVPVKETYNCWFGFDGVEAGRGWGAPNFSSLSDLEEPTKGYLLNDTLVTEVK 323

Query: 316 INVISTSKKI 320
           INVIST KKI
Sbjct: 324 INVISTLKKI 332

BLAST of Spg006896 vs. NCBI nr
Match: KAE8648447.1 (hypothetical protein Csa_008888 [Cucumis sativus])

HSP 1 Score: 388.7 bits (997), Expect = 5.0e-104
Identity = 198/311 (63.67%), Postives = 239/311 (76.85%), Query Frame = 0

Query: 16  AVVRSSRDQKPLHYTLQIQSFSLLKAALASSERDRYESQTFNAGGYKWKLALYPNGDEKR 75
           +VV SSR +KP+HY L+IQSFSLLK  LA S R R+ESQ FNAGGY+WKLALYPNGD++R
Sbjct: 27  SVVTSSRYEKPVHYILKIQSFSLLKEGLACSPRQRFESQKFNAGGYEWKLALYPNGDQRR 86

Query: 76  NGSDHISLYLVMAEDDVLWTTTSEVNVVFTFLVYNHLKDKYLIVQDGKVRRFHAIRKEWG 135
           + SDHISLYLVM  D++L +TTSEVN VFTFLVY+ L+ KYL VQDGK+RRF A + EWG
Sbjct: 87  DVSDHISLYLVMVGDNIL-STTSEVNAVFTFLVYDTLRGKYLTVQDGKMRRFSATKTEWG 146

Query: 136 FEKLVSLDTFNDVSNGLLVDDCCVFGVDVIVMKCD-GNGEVISLIKQPKNYKYTWKFTNF 195
            EKL+ L+TF D SNG LVDDCCVFGVD+ VM  D G GEV SLI+QP NYKYTWK  NF
Sbjct: 147 IEKLLPLNTFKDASNGFLVDDCCVFGVDIFVMNSDVGKGEVFSLIEQPNNYKYTWKLNNF 206

Query: 196 SQKKLNLYESDPFTVENYCWKIRLYPRGDSKSKTGYLSLYLMFDSYKELPQGFQVFVEWE 255
           S+   +L E +PFTVEN CWKIRL+P GD ++K G+ S+YLM  + KE PQG QV+VE+E
Sbjct: 207 SKLDSSLRECNPFTVENCCWKIRLFPSGDLQAKPGFFSMYLMLTNLKEFPQGAQVYVEYE 266

Query: 256 MSVLSQF-GVGHRTFTPKLWF-----EGEEGRGWPNFMSLSNLKEPKKGFLRDDTLIVEV 315
           M+VLSQ   V     T   WF     E ++G G  NF+SLS+LKEP +G+L +DTL++EV
Sbjct: 267 MAVLSQLEDVPPIKETYICWFGFGGIEADKGCGASNFLSLSDLKEPARGYLLNDTLVIEV 326

Query: 316 KINVISTSKKI 320
           KINVIST KKI
Sbjct: 327 KINVISTLKKI 336

BLAST of Spg006896 vs. NCBI nr
Match: XP_022146380.1 (probable inactive serine/threonine-protein kinase fnkC [Momordica charantia])

HSP 1 Score: 385.2 bits (988), Expect = 5.6e-103
Identity = 194/305 (63.61%), Postives = 226/305 (74.10%), Query Frame = 0

Query: 17  VVRSSRDQKPLHYTLQIQSFSLLKAALASSERDRYESQTFNAGGYKWKLALYPNGDEKRN 76
           V+RS+RD+KPLHYTLQIQSFSLLKAALASS RDRYESQ FNAGGYKWKLA+YPNGD KRN
Sbjct: 8   VIRSTRDEKPLHYTLQIQSFSLLKAALASSNRDRYESQIFNAGGYKWKLAVYPNGDVKRN 67

Query: 77  GSDHISLYLVMAEDDVLWTTTSEVNVVFTFLVYNHLKDKYLIVQDGKVRRFHAIRKEWGF 136
           G+DHISLYLV A+DD+                             GKVRRFHA++ EWGF
Sbjct: 68  GADHISLYLVKADDDI------------------------NPCSHGKVRRFHAMKAEWGF 127

Query: 137 EKLVSLDTFNDVSNGLLVDDCCVFGVDVIVMKCDGNGEVISLIKQPKNYKYTWKFTNFSQ 196
           EKL+SLDTFND SNG +V DCC FGVDV+VMKCDG GEV+S IK+PK++KYTWK  NFSQ
Sbjct: 128 EKLISLDTFNDSSNGFVVGDCCGFGVDVVVMKCDGKGEVVSFIKEPKSHKYTWKTKNFSQ 187

Query: 197 KKLNLYESDPFTVENYCWKIRLYPRGDSKSKTGYLSLYLMFDSYKELPQGFQVFVEWEMS 256
                YESDPFTVE Y WK+ LYP+G SK+KTG+LSLYL ++SY+E+PQG QV+VE+E S
Sbjct: 188 LDKKFYESDPFTVEGYRWKLWLYPKGSSKAKTGFLSLYLSYNSYEEIPQGSQVYVEYEDS 247

Query: 257 VLSQFGVGHRTFTPKLWFEGEEGRGW--PNFMSLSNLKEPKKGFLRDDTLIVEVKINVIS 316
               FG G            E GRGW  PNF+SL +L EP +GF+ DDT+IVEVKINV+S
Sbjct: 248 HNCWFGFG----------GVESGRGWGHPNFLSLRDLNEPTEGFIVDDTIIVEVKINVVS 278

Query: 317 TSKKI 320
           T KKI
Sbjct: 308 TLKKI 278

BLAST of Spg006896 vs. NCBI nr
Match: XP_022146382.1 (uncharacterized protein LOC111015610 [Momordica charantia])

HSP 1 Score: 368.2 bits (944), Expect = 7.0e-98
Identity = 188/305 (61.64%), Postives = 228/305 (74.75%), Query Frame = 0

Query: 16  AVVRSSRDQKPLHYTLQIQSFSLLKAALASSERDRYESQTFNAGGYKWKLALYPNGDEKR 75
           +V+RS RD+KPLHYTLQIQ FSLLK ALA+S RDRYES  F+  G+KWKLA+YP+GD KR
Sbjct: 52  SVLRSIRDEKPLHYTLQIQPFSLLKTALATSSRDRYESPIFSVAGFKWKLAVYPSGDVKR 111

Query: 76  NGSDHISLYLVMAEDDVLWTTTSEVNVVFTFLVYNHLKDKYLIVQDGKVRRFHAIRKEWG 135
           NGS+HISLYLV AEDD+    T ++ VVFT LVY++L+D YL V DGKVRRFHA++KEWG
Sbjct: 112 NGSNHISLYLVTAEDDI---PTLDLTVVFTILVYDNLRDNYLAVLDGKVRRFHAMKKEWG 171

Query: 136 FEKLVSLDTFNDVSNGLLVDDCCVFGVDVIVMKCDGNGEVISLIKQPKNYKYTWKFTNFS 195
           FEKLVSL+TFND SNG LVDDCC FGVD+ VMKCDG GE +SLIKQPKN KYTWK  NFS
Sbjct: 172 FEKLVSLETFNDSSNGFLVDDCCAFGVDIFVMKCDGKGEALSLIKQPKNNKYTWKTNNFS 231

Query: 196 QKKLNLYESDPFTVENYCWKIRLYPRGDSKSKTGYLSLYLMFDSYKELPQGFQVFVEWEM 255
           Q   N+             K+ LYP+G  K+   YLSL+L  +S KELPQG +V+ E+E+
Sbjct: 232 QLDENM-------------KMSLYPKGSPKASPDYLSLFLRCESLKELPQGCRVYAEFEI 291

Query: 256 SVLSQFGVGHRTFTPKLWFE-GEEGRGWPNFMSLSNLKEPKKGFLRDDTLIVEVKINVIS 315
           +VLS           K WFE G+ G G+P+FMSL +L E  KG++ DDTLIVEVKINV+S
Sbjct: 292 AVLS-----------KYWFEFGDLGLGFPDFMSLRDLNEVTKGYICDDTLIVEVKINVVS 329

Query: 316 TSKKI 320
           T K++
Sbjct: 352 TLKEL 329

BLAST of Spg006896 vs. ExPASy Swiss-Prot
Match: Q9FPT1 (Ubiquitin C-terminal hydrolase 12 OS=Arabidopsis thaliana OX=3702 GN=UBP12 PE=1 SV=2)

HSP 1 Score: 80.5 bits (197), Expect = 3.8e-14
Identity = 42/135 (31.11%), Postives = 75/135 (55.56%), Query Frame = 0

Query: 180 KQPKNYKYTWKFTNFSQKKLNLYESDPFTVENYCWKIRLYPRGDSKSKTGYLSLYLMFDS 239
           + P   K+TW   NFS++    + SD F V  Y W+I ++P+G++     +LS+YL    
Sbjct: 50  EDPPTLKFTWTIPNFSRQNTRKHYSDVFVVGGYKWRILIFPKGNN---VDHLSMYLDVSD 109

Query: 240 YKELPQGFQVFVEWEMSVLSQFGVGH--RTFTPKLWFEGEEGRGWPNFMSLSNLKEPKKG 299
              LP G+  + ++ ++V++Q    +  R  T   +   E   G+ +FM LS L +P +G
Sbjct: 110 AASLPYGWSRYAQFSLAVVNQIHTRYTVRKETQHQFNARESDWGFTSFMPLSELYDPSRG 169

Query: 300 FLRDDTLIVEVKINV 313
           +L +DT++VE ++ V
Sbjct: 170 YLVNDTVLVEAEVAV 181

BLAST of Spg006896 vs. ExPASy Swiss-Prot
Match: Q84WU2 (Ubiquitin C-terminal hydrolase 13 OS=Arabidopsis thaliana OX=3702 GN=UBP13 PE=1 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 4.7e-12
Identity = 40/135 (29.63%), Postives = 74/135 (54.81%), Query Frame = 0

Query: 180 KQPKNYKYTWKFTNFSQKKLNLYESDPFTVENYCWKIRLYPRGDSKSKTGYLSLYLMFDS 239
           + P + K+TW    F++     + SD F V  Y W+I ++P+G++     +LS+YL    
Sbjct: 49  EDPPSLKFTWTIPMFTRLNTRKHYSDVFVVGGYKWRILIFPKGNN---VDHLSMYLDVAD 108

Query: 240 YKELPQGFQVFVEWEMSVLSQFGVGH--RTFTPKLWFEGEEGRGWPNFMSLSNLKEPKKG 299
              LP G+  + ++ ++V++Q    +  R  T   +   E   G+ +FM LS L EP +G
Sbjct: 109 AANLPYGWSRYSQFSLAVVNQVNNRYSIRKETQHQFNARESDWGFTSFMPLSELYEPTRG 168

Query: 300 FLRDDTLIVEVKINV 313
           +L +DT+++E ++ V
Sbjct: 169 YLVNDTVLIEAEVAV 180

BLAST of Spg006896 vs. ExPASy Swiss-Prot
Match: Q9M2H9 (MATH domain and coiled-coil domain-containing protein At3g58370 OS=Arabidopsis thaliana OX=3702 GN=At3g58370 PE=4 SV=3)

HSP 1 Score: 64.3 bits (155), Expect = 2.8e-09
Identity = 40/143 (27.97%), Postives = 72/143 (50.35%), Query Frame = 0

Query: 186 KYTWKFTNFSQKKLNLYESDPFTVENYCWKIRLYPRGDSKSKTGYLSLYLMFDSYKELPQ 245
           K+TW   NFS  + +   SDPF +    W +  YP+G+  + +  LSLYL+ D  + LP 
Sbjct: 9   KFTWVIKNFSTLQYDKIYSDPFVIGGCKWHLLAYPKGNKFNNS--LSLYLVVDDARALPC 68

Query: 246 GFQVFVEWEMSVLSQFGVGHRTFTPKLWFEGEE---------GRGWPNFMSLSNLKEPKK 305
           G++ + ++ +++++Q        T KL   GE+         G G+ + + L NL     
Sbjct: 69  GWRRYAQFSLTIINQ-------LTDKLSQRGEKQNWFNQRNLGSGFTSMIPLPNLHAKNA 128

Query: 306 GFLRDDTLIVEVKINVISTSKKI 320
           G+L +  + + V+IN +    K+
Sbjct: 129 GYLVNGEVKIVVEINDLEVIGKL 142

BLAST of Spg006896 vs. ExPASy Swiss-Prot
Match: Q9C870 (MATH domain and coiled-coil domain-containing protein At1g31390 OS=Arabidopsis thaliana OX=3702 GN=At1g31390 PE=4 SV=2)

HSP 1 Score: 62.0 bits (149), Expect = 1.4e-08
Identity = 35/135 (25.93%), Postives = 67/135 (49.63%), Query Frame = 0

Query: 186 KYTWKFTNFSQKKLNLYESDPFTVENYCWKIRLYPRGDSKSKTGYLSLYLMFDSYKELPQ 245
           K TW   NFS  +    +SD F V +  W +  YP+G+ +S    LSLYL    ++ LP 
Sbjct: 8   KITWTIKNFSFVQSQAIDSDIFVVGDSKWHLVAYPKGNGESTNKCLSLYLNVADFQSLPN 67

Query: 246 GFQVFVEWEMSVLSQFG--VGHRTFTPKLWFEGEEGRGWPNFMSLSNLKEPKKGFLRDDT 305
           G++  +++ ++V++Q    +  +      +++     G+   + LS L +   GFL +  
Sbjct: 68  GWKRHIKYRLTVVNQMSEKLSEQEVIQGWFYKNFHISGFQTMLPLSKLLDKNGGFLVNGD 127

Query: 306 LIVEVKINVISTSKK 319
           + + V++ V+    K
Sbjct: 128 VKIVVEVGVLEVVGK 142

BLAST of Spg006896 vs. ExPASy Swiss-Prot
Match: Q8T126 (Probable inactive serine/threonine-protein kinase fnkC OS=Dictyostelium discoideum OX=44689 GN=fnkC PE=3 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 1.2e-07
Identity = 64/282 (22.70%), Postives = 116/282 (41.13%), Query Frame = 0

Query: 45   SSERDRYESQTFNAGGYKWKLALYPNGDEKRNGSDHISLYLVMAEDDVL---WTTTSEVN 104
            S+ +D++ S  F+  G  W+   Y NG   ++ S    L + ++  D+L   +T   E +
Sbjct: 1036 SNRKDQFYSPIFSLIGSNWRCKFYSNG---KDASTSGKLSIFISNCDLLNNPFTIFLEKS 1095

Query: 105  VVFTFLVYNHLKDKYLIVQDGKVRRFHAIRKEWGFEKLVSLDTFNDVSNGLLVDDCCVFG 164
            + +   + N  K+    +Q      F       G+   + L +  + +NG LV++     
Sbjct: 1096 ISYKLTLINQ-KNPNESIQKSSSHTFSIKEFNHGYGSFIGLFSLLNPNNGFLVNNTIKVR 1155

Query: 165  VDVIVMKCDGNGEVISLIKQPKNYKYTWKFTNFSQKKLNLYESDPFTVENYCWKIRLYPR 224
            +D        N      I   + + Y+        KK   + S  F      W I++YP 
Sbjct: 1156 IDAAPTSPLVNTYDKYNIGLNQAFSYS---VPMMSKKSEPFISPIFMSCGRKWIIKIYPM 1215

Query: 225  GDSKSKTGYLSLYLMFDSYKELPQGFQVFVEWEMSVLSQFGVGHRTFTPKLWFE-----G 284
            G   S   Y+S++L +    E        V + + ++SQ    +   + K W +      
Sbjct: 1216 GQPSS--NYMSVFLEYRDEGE------ENVHFSLELISQL---YPEQSIKYWVQYRFNSK 1275

Query: 285  EEGRGWPNFMSLSNLKEPKKGFLRDDTLIVEVKINVISTSKK 319
                G+P F+ +S L +P  GFL +DT+I+ V I  +   KK
Sbjct: 1276 SNSFGYPKFIGVSTLMDPDMGFLVNDTIILNVSILQLKPIKK 1299

BLAST of Spg006896 vs. ExPASy TrEMBL
Match: A0A6J1CX47 (probable inactive serine/threonine-protein kinase fnkC OS=Momordica charantia OX=3673 GN=LOC111015608 PE=4 SV=1)

HSP 1 Score: 385.2 bits (988), Expect = 2.7e-103
Identity = 194/305 (63.61%), Postives = 226/305 (74.10%), Query Frame = 0

Query: 17  VVRSSRDQKPLHYTLQIQSFSLLKAALASSERDRYESQTFNAGGYKWKLALYPNGDEKRN 76
           V+RS+RD+KPLHYTLQIQSFSLLKAALASS RDRYESQ FNAGGYKWKLA+YPNGD KRN
Sbjct: 8   VIRSTRDEKPLHYTLQIQSFSLLKAALASSNRDRYESQIFNAGGYKWKLAVYPNGDVKRN 67

Query: 77  GSDHISLYLVMAEDDVLWTTTSEVNVVFTFLVYNHLKDKYLIVQDGKVRRFHAIRKEWGF 136
           G+DHISLYLV A+DD+                             GKVRRFHA++ EWGF
Sbjct: 68  GADHISLYLVKADDDI------------------------NPCSHGKVRRFHAMKAEWGF 127

Query: 137 EKLVSLDTFNDVSNGLLVDDCCVFGVDVIVMKCDGNGEVISLIKQPKNYKYTWKFTNFSQ 196
           EKL+SLDTFND SNG +V DCC FGVDV+VMKCDG GEV+S IK+PK++KYTWK  NFSQ
Sbjct: 128 EKLISLDTFNDSSNGFVVGDCCGFGVDVVVMKCDGKGEVVSFIKEPKSHKYTWKTKNFSQ 187

Query: 197 KKLNLYESDPFTVENYCWKIRLYPRGDSKSKTGYLSLYLMFDSYKELPQGFQVFVEWEMS 256
                YESDPFTVE Y WK+ LYP+G SK+KTG+LSLYL ++SY+E+PQG QV+VE+E S
Sbjct: 188 LDKKFYESDPFTVEGYRWKLWLYPKGSSKAKTGFLSLYLSYNSYEEIPQGSQVYVEYEDS 247

Query: 257 VLSQFGVGHRTFTPKLWFEGEEGRGW--PNFMSLSNLKEPKKGFLRDDTLIVEVKINVIS 316
               FG G            E GRGW  PNF+SL +L EP +GF+ DDT+IVEVKINV+S
Sbjct: 248 HNCWFGFG----------GVESGRGWGHPNFLSLRDLNEPTEGFIVDDTIIVEVKINVVS 278

Query: 317 TSKKI 320
           T KKI
Sbjct: 308 TLKKI 278

BLAST of Spg006896 vs. ExPASy TrEMBL
Match: A0A6J1CZF7 (uncharacterized protein LOC111015610 OS=Momordica charantia OX=3673 GN=LOC111015610 PE=4 SV=1)

HSP 1 Score: 368.2 bits (944), Expect = 3.4e-98
Identity = 188/305 (61.64%), Postives = 228/305 (74.75%), Query Frame = 0

Query: 16  AVVRSSRDQKPLHYTLQIQSFSLLKAALASSERDRYESQTFNAGGYKWKLALYPNGDEKR 75
           +V+RS RD+KPLHYTLQIQ FSLLK ALA+S RDRYES  F+  G+KWKLA+YP+GD KR
Sbjct: 52  SVLRSIRDEKPLHYTLQIQPFSLLKTALATSSRDRYESPIFSVAGFKWKLAVYPSGDVKR 111

Query: 76  NGSDHISLYLVMAEDDVLWTTTSEVNVVFTFLVYNHLKDKYLIVQDGKVRRFHAIRKEWG 135
           NGS+HISLYLV AEDD+    T ++ VVFT LVY++L+D YL V DGKVRRFHA++KEWG
Sbjct: 112 NGSNHISLYLVTAEDDI---PTLDLTVVFTILVYDNLRDNYLAVLDGKVRRFHAMKKEWG 171

Query: 136 FEKLVSLDTFNDVSNGLLVDDCCVFGVDVIVMKCDGNGEVISLIKQPKNYKYTWKFTNFS 195
           FEKLVSL+TFND SNG LVDDCC FGVD+ VMKCDG GE +SLIKQPKN KYTWK  NFS
Sbjct: 172 FEKLVSLETFNDSSNGFLVDDCCAFGVDIFVMKCDGKGEALSLIKQPKNNKYTWKTNNFS 231

Query: 196 QKKLNLYESDPFTVENYCWKIRLYPRGDSKSKTGYLSLYLMFDSYKELPQGFQVFVEWEM 255
           Q   N+             K+ LYP+G  K+   YLSL+L  +S KELPQG +V+ E+E+
Sbjct: 232 QLDENM-------------KMSLYPKGSPKASPDYLSLFLRCESLKELPQGCRVYAEFEI 291

Query: 256 SVLSQFGVGHRTFTPKLWFE-GEEGRGWPNFMSLSNLKEPKKGFLRDDTLIVEVKINVIS 315
           +VLS           K WFE G+ G G+P+FMSL +L E  KG++ DDTLIVEVKINV+S
Sbjct: 292 AVLS-----------KYWFEFGDLGLGFPDFMSLRDLNEVTKGYICDDTLIVEVKINVVS 329

Query: 316 TSKKI 320
           T K++
Sbjct: 352 TLKEL 329

BLAST of Spg006896 vs. ExPASy TrEMBL
Match: A0A6J1CZ69 (ubiquitin carboxyl-terminal hydrolase 13-like OS=Momordica charantia OX=3673 GN=LOC111015609 PE=4 SV=1)

HSP 1 Score: 364.8 bits (935), Expect = 3.8e-97
Identity = 190/303 (62.71%), Postives = 220/303 (72.61%), Query Frame = 0

Query: 16  AVVRSSRDQKPLHYTLQIQSFSLLKAALASSERDRYESQTFNAGGYKWKLALYPNGDEKR 75
           +V+  +RD+KPLHYTLQIQSFSLLK ALA+S  DRYESQ FNAGGYKWKLA+YPNGD KR
Sbjct: 16  SVLSFTRDEKPLHYTLQIQSFSLLKTALAASNCDRYESQIFNAGGYKWKLAVYPNGDVKR 75

Query: 76  NGSDHISLYLVMAEDDVLWTTTSEVNVVFTFLVYNHLKDKYLIVQDGKVRRFHAIRKEWG 135
           NGSDHISLYLV AED +    TSEVNVVFTFLVY++L DKYL VQD KVRRFH+I+ EW 
Sbjct: 76  NGSDHISLYLVKAEDGI---PTSEVNVVFTFLVYDNLNDKYLAVQDEKVRRFHSIQTEWE 135

Query: 136 FEKLVSLDTFNDVSNGLLVDDCCVFGVDVIVMKCDGNGEVISLIKQPKNYKYTWKFTNFS 195
           FEKL+SL+TFND SNG LV DCC FGVD++V KCDG GEV+S IK+PKN +YTWK  NFS
Sbjct: 136 FEKLISLETFNDSSNGFLVGDCCAFGVDIVVKKCDGKGEVVSFIKEPKNNEYTWKTNNFS 195

Query: 196 QKKLNLYESDPFTVENYCWKIRLYPRGDSKSKTGYLSLYLMFDSYKELPQGFQVFVEWEM 255
           Q   N+YESDPFTVE Y                           +KELPQG QV+ E+E+
Sbjct: 196 QLDKNMYESDPFTVEGY-------------------------RCFKELPQGSQVYTEFEI 255

Query: 256 SVLSQFGVGHRTFTPKLWFE-GEEGRGWPNFMSLSNLKEPKKGFLRDDTLIVEVKINVIS 315
           +VLS           K WFE G+ G G+ NFMSL +L E  KG++ DDTLIVEVKINV+S
Sbjct: 256 AVLS-----------KYWFEFGDLGLGFRNFMSLRDLNEVTKGYICDDTLIVEVKINVVS 279

Query: 316 TSK 318
           T K
Sbjct: 316 TLK 279

BLAST of Spg006896 vs. ExPASy TrEMBL
Match: A0A1S3CQ20 (uncharacterized protein LOC103503506 OS=Cucumis melo OX=3656 GN=LOC103503506 PE=4 SV=1)

HSP 1 Score: 348.6 bits (893), Expect = 2.8e-92
Identity = 186/310 (60.00%), Postives = 230/310 (74.19%), Query Frame = 0

Query: 18  VRSSRDQKPLHYTLQIQSFSLLKAALASSERDRYESQTFNAGGYKWKLALYPNGDEKRNG 77
           V S+R +KP+HY L+IQSFSLLK A+ASS   R+ESQ F AGG +WKLALYPNGD +R+ 
Sbjct: 33  VTSTRYEKPIHYILKIQSFSLLKEAVASSPCQRFESQKFYAGGSEWKLALYPNGDRRRDV 92

Query: 78  SDHISLYLVMAEDDVLWTTTSEVNVVFTFLVYNHLKDKYLIVQDGKVRRFHAIRKEWGFE 137
           SDHISLYLV  E+++L +TTSEVNVVFTFLVY+ L+ KYL VQD K+R+F A ++EWG E
Sbjct: 93  SDHISLYLVRVENNIL-STTSEVNVVFTFLVYDTLRGKYLTVQDEKMRQFSATKREWGIE 152

Query: 138 KLVSLDTFNDVSNGLLVDDCCVFGVDVIVMKCD-GNGEVISLIKQPKNYKYTWKFTNFSQ 197
           KL+ L+TF+D SNG LVDDCCVFGVD+ VM C+   GEV SLIKQP NYKYTWK  NFS+
Sbjct: 153 KLLPLNTFSDASNGFLVDDCCVFGVDIFVMNCNVEKGEVFSLIKQPNNYKYTWKLDNFSK 212

Query: 198 -KKLNLYESDPFTVENYCWKIRLYPRGDSKSKTGYLSLYLMFDSYKELPQGFQVFVEWEM 257
               + +E + F VEN  WKIRL+P GD ++K G+ S+YLM  +    P+G QV+VE+EM
Sbjct: 213 LLDCSFWECNSFRVENCWWKIRLFPSGDWQAKPGFFSMYLMLSNLIAHPKGAQVYVEYEM 272

Query: 258 SVLSQF-GVGHRTFTPKLWF-----EGEEGRGWPNFMSLSNLKEPKKGFLRDDTLIVEVK 317
           +VLSQ   V     T   WF     E  +G G  NFMSLS+LKE  KG+L +DTL+VEVK
Sbjct: 273 AVLSQLEAVPPVKETYTCWFKSGCIEIYKGCGASNFMSLSDLKERTKGYLVNDTLVVEVK 332

Query: 318 INVISTSKKI 320
           INVIST KK+
Sbjct: 333 INVISTIKKL 341

BLAST of Spg006896 vs. ExPASy TrEMBL
Match: A0A6J1CXZ0 (ubiquitin carboxyl-terminal hydrolase 12-like OS=Momordica charantia OX=3673 GN=LOC111015607 PE=4 SV=1)

HSP 1 Score: 345.5 bits (885), Expect = 2.4e-91
Identity = 184/305 (60.33%), Postives = 214/305 (70.16%), Query Frame = 0

Query: 17  VVRSSRDQKPLHYTLQIQSFSLLKAALASSERDRYESQTFNAGGYKWKLALYPNGDEKRN 76
           V+RS+RD+KPLHYTLQIQSFSLLK AL  S+ DRYES+ FNAGGYKWKLA+YP GD KRN
Sbjct: 23  VIRSTRDEKPLHYTLQIQSFSLLKTALVRSDCDRYESRIFNAGGYKWKLAMYPTGDVKRN 82

Query: 77  GS-DHISLYLVMAEDDVLWTTTSEVNVVFTFLVYNHLKDKYLIVQDGKVRRFHAIRKEWG 136
           GS DHISLYLV AEDD+    TSEVNVVFTFLVY+ LKDKYL VQDG VRRFHAI+ EWG
Sbjct: 83  GSHDHISLYLVKAEDDI---PTSEVNVVFTFLVYDSLKDKYLAVQDGIVRRFHAIKTEWG 142

Query: 137 FEKLVSLDTFNDVSNGLLVDDCCVFGVDVIVMKCDGNGEVISLIKQPKNYKYTWKFTNFS 196
           FEKL+SLDTFND SNG LVDDCC FGVDV+VMK +G                        
Sbjct: 143 FEKLISLDTFNDSSNGFLVDDCCAFGVDVVVMKLEG------------------------ 202

Query: 197 QKKLNLYESDPFTVENYCWKIRLYPRGDSKSKTGYLSLYLMFDSYKELPQGFQVFVEWEM 256
                           Y WK+ LYP+G S ++TG+LSLYL+ DS KE+ QG +V+VE+E+
Sbjct: 203 ----------------YRWKLWLYPKGCSNAETGFLSLYLILDSSKEILQGSKVYVEYEL 262

Query: 257 SVLSQFGVGHRTFTPKLWFEGEEGRGWPNFMSLSNLKEPKKGFLRDDTLIVEVKINVIST 316
           ++LSQ G      T   WF G    G PNF+SLS LK+P KG+L +DTL VEVKINVIST
Sbjct: 263 ALLSQLGAEPEKETYNYWFAG----GGPNFISLSELKKPLKGYLHNDTLKVEVKINVIST 280

Query: 317 SKKIS 321
           SK  +
Sbjct: 323 SKSFN 280

BLAST of Spg006896 vs. TAIR 10
Match: AT3G17380.1 (TRAF-like family protein )

HSP 1 Score: 221.9 bits (564), Expect = 7.6e-58
Identity = 116/300 (38.67%), Postives = 172/300 (57.33%), Query Frame = 0

Query: 17  VVRSSRDQKPLHYTLQIQSFSLL-KAALASSERDRYESQTFNAGGYKWKLALYPNGDEKR 76
           +++S  D  P HY ++I+SFSLL K A+     +RYE+++F AGGYKWKL LYPNG++ +
Sbjct: 10  IIKSISDAPPTHYMVKIESFSLLTKHAI-----ERYETESFEAGGYKWKLVLYPNGNKSK 69

Query: 77  NGSDHISLYLVMAEDDVLWTTTSEVNVVFTFLVYNHLKDKYLIVQDGKVRRFHAIRKEWG 136
           N  DH+S+YL +A+   L +   EV  VF   + +  KD YLI+Q G  RRFH++++EWG
Sbjct: 70  NTKDHVSVYLSLADSSSL-SPGWEVYAVFRLYLLDQNKDNYLILQ-GNERRFHSVKREWG 129

Query: 137 FEKLVSLDTFNDVSNGLLVDDCCVFGVDVIVMK--CDGNGEVISLIKQPKNYKYTWKFTN 196
           F+K +   TF+D SNG L++D C+FG DV V K    G GE +S+IK   + K+ WK  N
Sbjct: 130 FDKFIPTGTFSDASNGYLMEDTCMFGADVFVSKERRSGRGECLSMIKDATSSKHVWKIEN 189

Query: 197 FSQKKLNLYESDPFTVENYCWKIRLYPRGDSKSKTGYLSLYLMFDSYKELPQGFQVFVEW 256
           FS+     Y+S+ F   +  WKI  YP G  +    +LS+YL     + +  G ++FVE+
Sbjct: 190 FSKLDKESYDSNAFFAGDRKWKIEFYPTGTKQGTGTHLSIYLTLVDPETISDGTKIFVEF 249

Query: 257 EMSVLSQFGVGHRTFTPKLWF-EGEEGRGWPNFMSLSNLKEPKKGFLRDDTLIVEVKINV 313
            + +  Q    H       WF       GW  ++S+    +P  G L  D  +VE  + V
Sbjct: 250 TIRIFDQLQGRHIAGKVTKWFSRSSSEHGWVKYVSMVYFTQPNSGLLLKDVCLVEADVCV 302

BLAST of Spg006896 vs. TAIR 10
Match: AT3G22080.1 (TRAF-like family protein )

HSP 1 Score: 189.1 bits (479), Expect = 5.4e-48
Identity = 103/302 (34.11%), Postives = 172/302 (56.95%), Query Frame = 0

Query: 18  VRSSRDQKPLHYTLQIQSFSLLKAALASSERDRYESQTFNAGGYKWKLALYPNGDEKRNG 77
           +RS R++ P  Y+L++Q+ S ++ +   S+  +Y+S+ F++GGY W++ +YP G+ K +G
Sbjct: 345 LRSWRERTPNSYSLKLQNISQVEKSTLFSD-GKYQSRLFSSGGYNWRMIIYPKGNRKDDG 404

Query: 78  SDHISLYLVMAEDDVLWTTTSEVNVVFTFLVYNHLKDKYLIVQDGKVRRFHAIRKEWGFE 137
           S  IS+Y+ +    +L T T+EV     F V+N  ++KY  +Q  + + F+A R  WG  
Sbjct: 405 SGFISMYVEIDSTSLLTTPTTEVFADLRFFVFNKKENKYYTIQHVESKLFNAFRTIWGLA 464

Query: 138 KLVSLDTFNDVSNGLLVD-DCCVFGVDVIVMKCDGNGEVISLIKQPKNYKYTWKFTNFSQ 197
           +++ +DTF D  NG + + D C FGVDVIV     N E+ +L +     K+ W   NFS+
Sbjct: 465 QVLPVDTFTDPKNGYIFEGDQCEFGVDVIVAAPPTNWEIHTLHEALSQPKFFWTVKNFSE 524

Query: 198 KKLNLYESDPFTVENYCWKIRLYPRGDSKSKTGYLSLYLMFDSYKELPQGFQVFVEWEMS 257
              N+Y S  F++    W ++LYP+GD K    +LSLYL  D  + L +  ++FV+ ++ 
Sbjct: 525 LNNNVYTSGNFSMRERKWVLKLYPKGDVKGDRKWLSLYLYLDQSETLKESEKIFVQAQLR 584

Query: 258 VLSQFGVGHRTFTPKLWF-EGEEGRGWPNFMSLSNLKEPKKGFLRDDTLIVEVKINVIST 317
           VL   G  H T     W+       G+  F+SL+   E  K +L  DTL V++ + V+S 
Sbjct: 585 VLDPRGSNHVTHKISSWYTSSNTAWGYRKFVSLA---EIPKAYLDKDTLKVQIDVEVVSE 642

BLAST of Spg006896 vs. TAIR 10
Match: AT3G20360.1 (TRAF-like family protein )

HSP 1 Score: 180.3 bits (456), Expect = 2.5e-45
Identity = 110/309 (35.60%), Postives = 173/309 (55.99%), Query Frame = 0

Query: 16  AVVRSSRDQKPLHYTLQIQSFSLLKAALASSERDRYESQTFNAGGYKWKLALYPNGDEKR 75
           + V+  R++ P  Y+L+++SF+ L   + S   +RYES+ F  G Y W L +YP G++  
Sbjct: 56  STVKGLRERPPSSYSLKMESFNTL---MKSVYTERYESRPFRVGRYNWTLVVYPKGNKND 115

Query: 76  NGSDHISLYLVMAEDDVLWTTTSEVNVVFTFLVYNHLKDKYLIVQDGKVRRFHAIRKEWG 135
           NG+ HISLY+V+ ++  L + + EV+V   F V+N  + KY  +QD  V RF AI++ WG
Sbjct: 116 NGTGHISLYVVL-DNSTLTSQSEEVHVDLRFYVFNKKETKYFTIQDTDVWRFSAIKRMWG 175

Query: 136 FEKLVSLDTFNDVSNGLLVD-DCCVFGVDVIVMKCDGNGEVISLIKQPKNYKYTWKFTNF 195
           F K++ L TFN++ NG L D D C FGVDVI+       EV S+ K   + ++TW    +
Sbjct: 176 FSKVLPLITFNNLKNGYLYDVDHCEFGVDVIIPPFYEKSEVFSVTKSFPSPRFTWYIQGY 235

Query: 196 SQKKLNLYESDPFTVENYCWKIRLYPRGDSKSKTGYLSLYLMF---DSYKELPQGFQVFV 255
           S    + Y S+ F +    W +R++  G    +   LSLYL     +  K  P   +V+V
Sbjct: 236 STLPTD-YLSEEFIIGGKSWNLRIFKNGFGAFEGKNLSLYLNLGPQELLKAKPYD-KVYV 295

Query: 256 EWEMSVLSQFGVGHRTFTPK---LWFEGEE-GRGWPNFMSLSNLKEPKKGFLRDDTLIVE 315
             ++ V +QFG        +    WF  +  G G+ +FM LS+L+   KGFL +D L+V+
Sbjct: 296 RAKLRVPNQFGSQSNLVLERPLDNWFSPQTIGWGYADFMPLSDLRNSSKGFLVNDMLVVQ 355

Query: 316 VKINVISTS 317
           V +  IS++
Sbjct: 356 VAMEEISST 358

BLAST of Spg006896 vs. TAIR 10
Match: AT5G26280.1 (TRAF-like family protein )

HSP 1 Score: 172.9 bits (437), Expect = 4.0e-43
Identity = 100/309 (32.36%), Postives = 155/309 (50.16%), Query Frame = 0

Query: 17  VVRSSRDQKPLHYTLQIQSFSLLKAALASSERDRYESQTFNAGGYKWKLALYPNGDEKRN 76
           V R  R+++P    + I SFS++K        + YES  F A GYKW+L LY NG++   
Sbjct: 50  VTRELREERPSSKIVTITSFSVIK-----GRGEPYESSVFEAAGYKWRLVLYVNGNKNDG 109

Query: 77  GSDHISLYLVMAEDDVL---WTTTSEVNVVFTFLVYNHLKDKYLIVQDGKVRRFHAIRKE 136
           G+DHISLY  + E + L   W    EVNV     V+N    KYL V DG V+R++  +KE
Sbjct: 110 GNDHISLYARIEETNSLPLGW----EVNVDLKLFVHNGKLHKYLTVTDGLVKRYNNAKKE 169

Query: 137 WGFEKLVSLDTFNDVSNGLLVDDCCVFGVDVIVMKCDGNGEVISLIKQPKNYKYTWKFTN 196
           WGF +L+   TF + + G L  D   FG ++ ++K     E ++ I  P N  +TWK   
Sbjct: 170 WGFGQLIPRSTFYNANEGYLDQDTGSFGAEIFIVKPAQQQEKVTFISNPPNNVFTWKILR 229

Query: 197 FSQKKLNLYESDPFTVENYCWKIRLYPRGDSKSKTGYLSLYLMFDSYKELPQGFQVFVEW 256
           FS  +   Y SD F VE+  W++   P+GD   +   L ++L    +K        +   
Sbjct: 230 FSTLEDKFYYSDDFLVEDRYWRLGFNPKGDGGGRPHALPIFLFAQGHKANAVATNTWGAV 289

Query: 257 EMSVLSQFGVGHRTFTPKLWFE--GEEGRGWPNFMSLSNLKEPKKGFLRDDTLIVEVKIN 316
            + + +Q    HR      W+      G G  N + L++L +  KG+L +D +I E ++ 
Sbjct: 290 NLRLKNQRSTNHRQIYSAAWYPIGSGYGVGVNNIILLADLNDASKGYLVNDAIIFEAEMV 349

Query: 317 VISTSKKIS 321
            +S +  +S
Sbjct: 350 KVSITNIVS 349

BLAST of Spg006896 vs. TAIR 10
Match: AT2G04170.1 (TRAF-like family protein )

HSP 1 Score: 171.8 bits (434), Expect = 9.0e-43
Identity = 97/300 (32.33%), Postives = 154/300 (51.33%), Query Frame = 0

Query: 22  RDQKPLHYTLQIQSFSLLKAALASSERDRYESQTFNAGGYKWKLALYPNGDEKRNGSDHI 81
           RD++P +  L I SFS++K        + YES  F AGGYKW+L LY NG++   G++HI
Sbjct: 125 RDERPSNKILTITSFSVIK-----GRGEPYESSVFEAGGYKWRLVLYVNGNQNDGGNNHI 184

Query: 82  SLYLVMAEDDVL---WTTTSEVNVVFTFLVYNHLKDKYLIVQDGKVRRFHAIRKEWGFEK 141
           SLY+ + E + L   W    EVNV     VYN  + KYLIV+DG V+R++  +KEWG+ K
Sbjct: 185 SLYVRIEETESLPKGW----EVNVELKLFVYNGKQRKYLIVKDGIVKRYNDAKKEWGYGK 244

Query: 142 LVSLDTFNDVSNGLLVDDCCVFGVDVIVMKCDGNGEVISLIKQPKNYKYTWKFTNFSQKK 201
           L+ L TF D + G L  D   FG ++         E ++ I  P N  +TWK  +FS  +
Sbjct: 245 LIPLTTFLDTNEGYLEQDIASFGAEIFSGTAVQVQEKVTFISNPPNNVFTWKILHFSNLE 304

Query: 202 LNLYESDPFTVENYCWKIRLYPRGDSKSKTGYLSLYLMFDSYKELPQGFQVFVEWEMSVL 261
              Y SD F VE+  W++   P+G    ++  + ++L    +K        +    + + 
Sbjct: 305 DKFYYSDDFLVEDRYWRLGFNPKGTGDGRSQAIPIFLYAQGHKPNAVATNTWGAVNLRLK 364

Query: 262 SQFGVGHRTFTPKLWF--EGEEGRGWPNFMSLSNLKEPKKGFLRDDTLIVEVKINVISTS 317
           +Q    H       W+    + G G    +SL+   +  KG+  +D++I E ++  +S +
Sbjct: 365 NQRSSNHAQIYSAAWYPTRSDYGVGVNTIISLAEFNDASKGYSVNDSIIFEAEMVKVSVT 415