Spg003495 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg003495
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptioncalcium uptake protein, mitochondrial-like
Locationscaffold4: 46824362 .. 46827419 (-)
RNA-Seq ExpressionSpg003495
SyntenySpg003495
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAAACCGTTATGACCAATGGCTCAGTCAAAGCCCCCAATTTCGTATCCAATTTTGCCAGCTTATTTATCTCCCTCCCTTTCTCTTTCTCATTTTGCAATTTTTTTTCTCCTTCCTTACCTTTTTCTTGAAGACCTTGATGACATTTTGAAGAACCAGAACCTCGAGATCGATTCGAATCATAGTATGTTTTCTTCGACGTCCTCGGTTGCAAAATCCTCTAAATCGATCTCTAGAGTTATACCTTCTCGACAACTTACCGCTCGGTCGCTCAAAAATCAACCTGATGGTTCTTTAGGATCGCCATCAACTTCTCCACTATCGTCTTCTTCTTTGTTGATCACATCTCAACAATCTGGCTCTGGGGGAGAGAATAGAAGAAGCTTCATATCCGACTTCTTGATCAAATCTATTTCTTCAGGGATTCTGATTGTGGGAGTTGGATCGCTTATTGGCTCAAATAATTCATCCACGGCCAATTCACATGTATCTTTTGCTGACTTCCCCAAACAAACATGGGCTGTGAGAGAAGACCAATTCCAATACCCATCACCAAATCAAAACGCGAAGAAGAAACCGAAGTTTCTATTTGGAGGTAAGAGTGTTTGTCGAAAACCCAAATGTTTTGAGCTAATGGGTTATGGTAAATTTAATTTTTGTGTTTGGATTCCAGATGATTACAGGAGGAAGGTGTTTTTTAACTACGAAAAACGCATGAGGCTCCAAAGTCCTCCAGAGAAGGTGTTCGAGTACTTTGCGTCGATCCACACACCCGAAGGAGAAATTTTTATGACACCTGCAGATCTGATGAGAGCCATTGTTCCTGTATTTCCTCCATCGGAGTCCAATCGTGTTAGGGAGGGGTTTCTAAGAGGAGAAAGAAGAGTTCATGGAGAGCTTTATTGTGTTCCTTCTAAATTTTTCATGCTTTTCGACACCAACAATGATGGACTCATCTCTTTTGCAGAGTATAGAGTTACTTCTACCTTTTTCTTTCTGATTGAAATTCTAGATGAATGATAAGTTTGTTTCGGGTTGAATCGGGCCATCGCTGTTTCAAGATAAATGAACCTAATTTACAAACCATCACTTTTTAAGTTAAAGGTGATGTGGTTTGTTTCTTGTGAATTACTTGCAGGTACATCTTTTTTGTTACACTTCTCAGCATACCAGAGTCAAGCTTTTCAGTGGCATTCAAAATGTTTGACATTGACAACAATGGGTTAGATAATCACCTCTATTTGTGTCATTTTCTTGTGTTTTTGTCTTTTCAACCAACAAAATGATGAAGTTTGTTCCATTGTGGTTATGGTTGTGTTGGTCAAAATAGTGCAATAGACAGGGAGGAGTTCAAGAAAGTTATGGGTTTGATGAGAAAACAGAATAGACAAGGAGCTCGTCATAGAGATGGAAGAAGGCTTGGGATGAAAGTTTGTGTTGAAAATGGTGGTTTGGTTGAGTATTTCTTTGGTCAAGATGGAAATGACTCCCTCCACCATGATAAATTTGTCCAATTTTTGAGACAGCTTCATGAAGAGGTATGATATTTCGATAAAACTTTGCATAATCGTGAGCAGGAAATTTATTGCTTAACATTGACTAAGGGATCAAAAGTTGAACACTCCAACCCCACATGTTGAACTCATAAGAAAAGTTGATTATGTCTTTAACATTACAAATGTGGGGCAAAAATTCCTATAATCAACACAAAACTTCAATGAAATTTGAACATTATGACTCCTATACCAAACTTCAAATTGGGTGAATGCAGATACTTCTACTAGAGTTCTCACATTATGACTTCAAGTCACAAGGAAGCATAACAGCCAAAGACTTTGCACTGTCCGTGGTAGCTTCAGCTGACATAAATCACATCGACAAATTACTGGACCGAGTTGAAGCATTGAACAAAGAACCCTATTTCAGAAACATCAGAATCACATATCAAGAATTCAAAGACTTTGCAGAGCTACGAAAAAAGTTAGAATCCTTCTCTTTGGCCATTTTCAGCTATGGAAAAGTAAATGGGGCGCTGACCAAGAAAGATTTCCAGAGAGCAGCTTCCCATGTAAGTACCAAAAACTTCAATCTGAAAGCTCAATTCAATAAGCTTAATCAGCCTAATAATCACATGCATGTGGCTTTTCTGTGCAAGGTCTGTGGAGTTTCAATCACTAGAAATGTGGTGGACATAATATTTCACATATTTGATGTAAATGCCGATGGGGATTTAAGCTCAGATGAGTTTGTAAGAGTCGTACAGAGAAGAGAAGTCAGTTCTTCACAAGCAGCTGTCGGTGTTGCAGGAATTCTGTCTTGCTGGTTCAGCTGTACAGCTAAATGCTCTTCTGCAAAGCTCTTCCTCCGCAGCTGAATAGAGAATCATCCATGGCAGCGTCCTGTTTCTGATGCCAGTTCCTCGACTTCTTTTCACTACTGATGTTCCTGTAAACATGAAAATTTCCCAACATGATGAAATACTTTCTGGGACTCTTCCTAGGGTTAAGAAAAAACGTAATCGAAGCTTTTCTTTTCTTTTATAAGTTCAATCTCGATTTTACTTAGAACCACAAAATCTTGTTCTCATGTTCTGAAAACAAAACGTAAAACGGTGCCTGAAAAAGAGAAATGAAAGAGAGGTGGCTCAGATCTGGAAATGGAGCAAAGCAGAGGAGGCCGGCCATGGCGGCGAACCCTAGCCGGATCGCCATGCCGATGGATCGGACCTCGTTCGCCATTGCCCTAAAATCCTCACACTCCAAAATCGAAATCATCATCATAGCCATTTTAAGGGAAAATTTCCGGCACTACCGGAGAATGGAGGAGACAGCGTAAAGAGAAAGAAAGAGAGAGAGAGAGAAAGAGATATACATATATATATATATACATACATACACAGAGAGAGAGAGAGAGATGAAGAGGGTGAGGATTCGGAGGGGAAATCACCGGCCGCCGGCGACACAGAGCGAGGAAAAGAAGAAACTCCTCCAGTCGCTCTGCAACGACGGGGCCTCATTCATGTCGGAGTGTGAGGCCGAACCCTAATTTTCAGCCCCCT

mRNA sequence

ATGGCTCAGTCAAAGCCCCCAATTTCGTATCCAATTTTGCCAGCTTATTTATCTCCCTCCCTTTCTCTTTCTCATTTTGCAATTTTTTTTCTCCTTCCTTACCTTTTTCTTGAAGACCTTGATGACATTTTGAAGAACCAGAACCTCGAGATCGATTCGAATCATAGTATGTTTTCTTCGACGTCCTCGGTTGCAAAATCCTCTAAATCGATCTCTAGAGTTATACCTTCTCGACAACTTACCGCTCGGTCGCTCAAAAATCAACCTGATGGTTCTTTAGGATCGCCATCAACTTCTCCACTATCGTCTTCTTCTTTGTTGATCACATCTCAACAATCTGGCTCTGGGGGAGAGAATAGAAGAAGCTTCATATCCGACTTCTTGATCAAATCTATTTCTTCAGGGATTCTGATTGTGGGAGTTGGATCGCTTATTGGCTCAAATAATTCATCCACGGCCAATTCACATGTATCTTTTGCTGACTTCCCCAAACAAACATGGGCTGTGAGAGAAGACCAATTCCAATACCCATCACCAAATCAAAACGCGAAGAAGAAACCGAAGTTTCTATTTGGAGATGATTACAGGAGGAAGGTGTTTTTTAACTACGAAAAACGCATGAGGCTCCAAAGTCCTCCAGAGAAGGTGTTCGAGTACTTTGCGTCGATCCACACACCCGAAGGAGAAATTTTTATGACACCTGCAGATCTGATGAGAGCCATTGTTCCTGTATTTCCTCCATCGGAGTCCAATCGTGTTAGGGAGGGGTTTCTAAGAGGAGAAAGAAGAGTTCATGGAGAGCTTTATTGTGTTCCTTCTAAATTTTTCATGCTTTTCGACACCAACAATGATGGACTCATCTCTTTTGCAGAGTACATCTTTTTTGTTACACTTCTCAGCATACCAGAGTCAAGCTTTTCAGTGGCATTCAAAATGTTTGACATTGACAACAATGGTGCAATAGACAGGGAGGAGTTCAAGAAAGTTATGGGTTTGATGAGAAAACAGAATAGACAAGGAGCTCGTCATAGAGATGGAAGAAGGCTTGGGATGAAAGTTTGTGTTGAAAATGGTGGTTTGGTTGAGTATTTCTTTGGTCAAGATGGAAATGACTCCCTCCACCATGATAAATTTGTCCAATTTTTGAGACAGCTTCATGAAGAGATACTTCTACTAGAGTTCTCACATTATGACTTCAAGTCACAAGGAAGCATAACAGCCAAAGACTTTGCACTGTCCGTGGTAGCTTCAGCTGACATAAATCACATCGACAAATTACTGGACCGAGTTGAAGCATTGAACAAAGAACCCTATTTCAGAAACATCAGAATCACATATCAAGAATTCAAAGACTTTGCAGAGCTACGAAAAAAGTTAGAATCCTTCTCTTTGGCCATTTTCAGCTATGGAAAAGTAAATGGGGCGCTGACCAAGAAAGATTTCCAGAGAGCAGCTTCCCATGTAAGTACCAAAAACTTCAATCTGAAAGCTCAATTCAATAAGCTTAATCAGCCTAATAATCACATGCATGTGGCTTTTCTGTGCAAGGTCTGTGGAGTTTCAATCACTAGAAATGTGGTGGACATAATATTTCACATATTTGATGTAAATGCCGATGGGGATTTAAGCTCAGATGAGTTTGTAAGAGTCGTACAGAGAAGAGAAGTCAGTTCTTCACAAGCAGCTGTCGGTGTTGCAGGAATTCTGTCTTGCTGGTTCAGCTGTACAGCTAAATGCTCTTCTGCAAAGCTCTTCCTCCGCAGCTGA

Coding sequence (CDS)

ATGGCTCAGTCAAAGCCCCCAATTTCGTATCCAATTTTGCCAGCTTATTTATCTCCCTCCCTTTCTCTTTCTCATTTTGCAATTTTTTTTCTCCTTCCTTACCTTTTTCTTGAAGACCTTGATGACATTTTGAAGAACCAGAACCTCGAGATCGATTCGAATCATAGTATGTTTTCTTCGACGTCCTCGGTTGCAAAATCCTCTAAATCGATCTCTAGAGTTATACCTTCTCGACAACTTACCGCTCGGTCGCTCAAAAATCAACCTGATGGTTCTTTAGGATCGCCATCAACTTCTCCACTATCGTCTTCTTCTTTGTTGATCACATCTCAACAATCTGGCTCTGGGGGAGAGAATAGAAGAAGCTTCATATCCGACTTCTTGATCAAATCTATTTCTTCAGGGATTCTGATTGTGGGAGTTGGATCGCTTATTGGCTCAAATAATTCATCCACGGCCAATTCACATGTATCTTTTGCTGACTTCCCCAAACAAACATGGGCTGTGAGAGAAGACCAATTCCAATACCCATCACCAAATCAAAACGCGAAGAAGAAACCGAAGTTTCTATTTGGAGATGATTACAGGAGGAAGGTGTTTTTTAACTACGAAAAACGCATGAGGCTCCAAAGTCCTCCAGAGAAGGTGTTCGAGTACTTTGCGTCGATCCACACACCCGAAGGAGAAATTTTTATGACACCTGCAGATCTGATGAGAGCCATTGTTCCTGTATTTCCTCCATCGGAGTCCAATCGTGTTAGGGAGGGGTTTCTAAGAGGAGAAAGAAGAGTTCATGGAGAGCTTTATTGTGTTCCTTCTAAATTTTTCATGCTTTTCGACACCAACAATGATGGACTCATCTCTTTTGCAGAGTACATCTTTTTTGTTACACTTCTCAGCATACCAGAGTCAAGCTTTTCAGTGGCATTCAAAATGTTTGACATTGACAACAATGGTGCAATAGACAGGGAGGAGTTCAAGAAAGTTATGGGTTTGATGAGAAAACAGAATAGACAAGGAGCTCGTCATAGAGATGGAAGAAGGCTTGGGATGAAAGTTTGTGTTGAAAATGGTGGTTTGGTTGAGTATTTCTTTGGTCAAGATGGAAATGACTCCCTCCACCATGATAAATTTGTCCAATTTTTGAGACAGCTTCATGAAGAGATACTTCTACTAGAGTTCTCACATTATGACTTCAAGTCACAAGGAAGCATAACAGCCAAAGACTTTGCACTGTCCGTGGTAGCTTCAGCTGACATAAATCACATCGACAAATTACTGGACCGAGTTGAAGCATTGAACAAAGAACCCTATTTCAGAAACATCAGAATCACATATCAAGAATTCAAAGACTTTGCAGAGCTACGAAAAAAGTTAGAATCCTTCTCTTTGGCCATTTTCAGCTATGGAAAAGTAAATGGGGCGCTGACCAAGAAAGATTTCCAGAGAGCAGCTTCCCATGTAAGTACCAAAAACTTCAATCTGAAAGCTCAATTCAATAAGCTTAATCAGCCTAATAATCACATGCATGTGGCTTTTCTGTGCAAGGTCTGTGGAGTTTCAATCACTAGAAATGTGGTGGACATAATATTTCACATATTTGATGTAAATGCCGATGGGGATTTAAGCTCAGATGAGTTTGTAAGAGTCGTACAGAGAAGAGAAGTCAGTTCTTCACAAGCAGCTGTCGGTGTTGCAGGAATTCTGTCTTGCTGGTTCAGCTGTACAGCTAAATGCTCTTCTGCAAAGCTCTTCCTCCGCAGCTGA

Protein sequence

MAQSKPPISYPILPAYLSPSLSLSHFAIFFLLPYLFLEDLDDILKNQNLEIDSNHSMFSSTSSVAKSSKSISRVIPSRQLTARSLKNQPDGSLGSPSTSPLSSSSLLITSQQSGSGGENRRSFISDFLIKSISSGILIVGVGSLIGSNNSSTANSHVSFADFPKQTWAVREDQFQYPSPNQNAKKKPKFLFGDDYRRKVFFNYEKRMRLQSPPEKVFEYFASIHTPEGEIFMTPADLMRAIVPVFPPSESNRVREGFLRGERRVHGELYCVPSKFFMLFDTNNDGLISFAEYIFFVTLLSIPESSFSVAFKMFDIDNNGAIDREEFKKVMGLMRKQNRQGARHRDGRRLGMKVCVENGGLVEYFFGQDGNDSLHHDKFVQFLRQLHEEILLLEFSHYDFKSQGSITAKDFALSVVASADINHIDKLLDRVEALNKEPYFRNIRITYQEFKDFAELRKKLESFSLAIFSYGKVNGALTKKDFQRAASHVSTKNFNLKAQFNKLNQPNNHMHVAFLCKVCGVSITRNVVDIIFHIFDVNADGDLSSDEFVRVVQRREVSSSQAAVGVAGILSCWFSCTAKCSSAKLFLRS
Homology
BLAST of Spg003495 vs. NCBI nr
Match: XP_008456564.1 (PREDICTED: calcium uptake protein 1, mitochondrial-like isoform X1 [Cucumis melo])

HSP 1 Score: 836.3 bits (2159), Expect = 1.7e-238
Identity = 444/537 (82.68%), Postives = 479/537 (89.20%), Query Frame = 0

Query: 57  MFSSTSSVAKSSKSISRVIPSRQLTARSLKNQPDGSLGSPSTSPLSSSSLLITSQQSGSG 116
           MFSST  V+KSSKSI RVIPS+ LT R L NQ  GSLGSPSTSPLSSSS  IT+QQ+G G
Sbjct: 1   MFSSTFLVSKSSKSICRVIPSQHLTVRFLNNQLGGSLGSPSTSPLSSSS-SITTQQTGCG 60

Query: 117 GENRRSFISDFLIKSISSGILIVGVGSLIGSNNSSTANSHVSFADFPKQ-TW-AVREDQF 176
           GENRR+F+ +FLIK ISSG+LI GVGSL+ S+ SST+NSH+SFADFPK+ TW  V+EDQF
Sbjct: 61  GENRRNFVFEFLIKLISSGLLIGGVGSLL-SSKSSTSNSHLSFADFPKETTWTTVKEDQF 120

Query: 177 QYPSPNQ--NAKKKPKFLFGDDYRRKVFFNYEKRMRLQSPPEKVFEYFASIHTPEGEIFM 236
           QYPSPNQ  NAKKK KFLFGDDYRR+VFFNYEKR+R+QSPPEKVFEYFASIHTPEGEI+M
Sbjct: 121 QYPSPNQNINAKKKSKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTPEGEIYM 180

Query: 237 TPADLMRAIVPVFPPSESNRVREGFLRGERRVHGELYCVPSKFFMLFDTNNDGLISFAEY 296
            P+DLMRAIVPVFPPSESNRVREGFLRGERR+ GEL C PSKFFMLFDTNNDGLISFAEY
Sbjct: 181 RPSDLMRAIVPVFPPSESNRVREGFLRGERRISGELCCAPSKFFMLFDTNNDGLISFAEY 240

Query: 297 IFFVTLLSIPESSFSVAFKMFDIDNNGAIDREEFKKVMGLMRKQNRQGARHRDGRRLGMK 356
           IFFVTLLSIPESSFSVAFKMFDIDNNG IDREEFKKVMGLMRKQNRQGA HRDGRR GMK
Sbjct: 241 IFFVTLLSIPESSFSVAFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRFGMK 300

Query: 357 VCVENGGLVEYFFGQDGNDSLHHDKFVQFLRQLHEEILLLEFSHYDFKSQGSITAKDFAL 416
           V VENGGLVEYFFG+DG  SLHHDKFVQFLRQLHEEIL+LEFSHYDFKSQGSI+ KDFAL
Sbjct: 301 VSVENGGLVEYFFGEDGKGSLHHDKFVQFLRQLHEEILILEFSHYDFKSQGSISVKDFAL 360

Query: 417 SVVASADINHIDKLLDRVEALNKEPYFRNIRITYQEFKDFAELRKKLESFSLAIFSYGKV 476
           S+VASADINHIDKLLDRVEALNKEP+F+NIRITY+EFKDFAELRKKLESFSLAIFSYGKV
Sbjct: 361 SIVASADINHIDKLLDRVEALNKEPHFKNIRITYEEFKDFAELRKKLESFSLAIFSYGKV 420

Query: 477 NGALTKKDFQRAASHVSTKNFNLKAQFNKLNQPNNH-MHVAFLCKVCGVSITRNVVDIIF 536
           NG LTK+DFQRAAS VST   +   Q NKL     H +   FL KVCGVSIT+NVVDIIF
Sbjct: 421 NGELTKQDFQRAASQVST---HQTFQVNKLKINLIHNVGFVFLYKVCGVSITKNVVDIIF 480

Query: 537 HIFDVNADGDLSSDEFVRVVQRREVSSSQAAVGVAGILSCWFSCTAKCSSAKLFLRS 589
           HIFD N DGDLSSDEFVRV+QRREV+SSQ AVGV G+LSCWFSC AKCSSAKLF+RS
Sbjct: 481 HIFDANEDGDLSSDEFVRVIQRREVNSSQPAVGVGGLLSCWFSCAAKCSSAKLFVRS 532

BLAST of Spg003495 vs. NCBI nr
Match: KAA0063559.1 (calcium uptake protein 1 [Cucumis melo var. makuwa] >TYJ97885.1 calcium uptake protein 1 [Cucumis melo var. makuwa])

HSP 1 Score: 819.7 bits (2116), Expect = 1.6e-233
Identity = 434/536 (80.97%), Postives = 468/536 (87.31%), Query Frame = 0

Query: 57  MFSSTSSVAKSSKSISRVIPSRQLTARSLKNQPDGSLGSPSTSPLSSSSLLITSQQSGSG 116
           MFSST  V+KSSKSI RVIPS+ LT R L NQ  GSLGSPSTSPLSSSS  IT+QQ+G G
Sbjct: 1   MFSSTFLVSKSSKSICRVIPSQHLTVRFLNNQLGGSLGSPSTSPLSSSS-SITTQQTGCG 60

Query: 117 GENRRSFISDFLIKSISSGILIVGVGSLIGSNNSSTANSHVSFADFPKQ-TW-AVREDQF 176
           GENRR+F+ +FLIK ISSGILI GVGSL+ S+NSST+NSH+SFA+FPK+ TW  V+EDQF
Sbjct: 61  GENRRNFVFEFLIKLISSGILIGGVGSLL-SSNSSTSNSHLSFAEFPKETTWTTVKEDQF 120

Query: 177 QYPSPNQ--NAKKKPKFLFGDDYRRKVFFNYEKRMRLQSPPEKVFEYFASIHTPEGEIFM 236
           QYPSPNQ  NAKKK KFLFGDDYRR+VFFNYEKR+R+QSPPEKVFEYFASIHTPEGEI+M
Sbjct: 121 QYPSPNQNINAKKKSKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTPEGEIYM 180

Query: 237 TPADLMRAIVPVFPPSESNRVREGFLRGERRVHGELYCVPSKFFMLFDTNNDGLISFAEY 296
            P+DLMRAIVPVFPPSESNRVREGFLRGERR+ GEL C PSKFFMLFDTNNDGLISFAEY
Sbjct: 181 RPSDLMRAIVPVFPPSESNRVREGFLRGERRISGELCCAPSKFFMLFDTNNDGLISFAEY 240

Query: 297 IFFVTLLSIPESSFSVAFKMFDIDNNGAIDREEFKKVMGLMRKQNRQGARHRDGRRLGMK 356
           IFFVTLLSIPESSFSVAFKMFDIDNNG IDREEFKKVMGLMRKQNRQGA HRDGRR GMK
Sbjct: 241 IFFVTLLSIPESSFSVAFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRFGMK 300

Query: 357 VCVENGGLVEYFFGQDGNDSLHHDKFVQFLRQLHEEILLLEFSHYDFKSQGSITAKDFAL 416
           V VENGGLVEYFFG+DG  SLHHDKFVQFLRQLHEEIL+LEFSHYDFKSQGSI+ KDFAL
Sbjct: 301 VSVENGGLVEYFFGEDGKGSLHHDKFVQFLRQLHEEILILEFSHYDFKSQGSISVKDFAL 360

Query: 417 SVVASADINHIDKLLDRVEALNKEPYFRNIRITYQEFKDFAELRKKLESFSLAIFSYGKV 476
           S+VASADINHIDKLLDRVEALNKEP+F+NIRITY+EFKDFAELRKKLESFSLAIFSYGKV
Sbjct: 361 SIVASADINHIDKLLDRVEALNKEPHFKNIRITYEEFKDFAELRKKLESFSLAIFSYGKV 420

Query: 477 NGALTKKDFQRAASHVSTKNFNLKAQFNKLNQPNNHMHVAFLCKVCGVSITRNVVDIIFH 536
           NG LTK+DFQRAAS                             +VCGVSIT+NVVDIIFH
Sbjct: 421 NGELTKQDFQRAAS-----------------------------QVCGVSITKNVVDIIFH 480

Query: 537 IFDVNADGDLSSDEFVRVVQRREVSSSQAAVGVAGILSCWFSCTAKCSSAKLFLRS 589
           IFD N DGDLSSDEFVRV+QRREV+SSQ AVGV G+LSCWFSC AKCSSAKLF+RS
Sbjct: 481 IFDANEDGDLSSDEFVRVIQRREVNSSQPAVGVGGLLSCWFSCAAKCSSAKLFVRS 505

BLAST of Spg003495 vs. NCBI nr
Match: XP_008456571.1 (PREDICTED: calcium uptake protein 1, mitochondrial-like isoform X2 [Cucumis melo])

HSP 1 Score: 818.1 bits (2112), Expect = 4.7e-233
Identity = 433/536 (80.78%), Postives = 467/536 (87.13%), Query Frame = 0

Query: 57  MFSSTSSVAKSSKSISRVIPSRQLTARSLKNQPDGSLGSPSTSPLSSSSLLITSQQSGSG 116
           MFSST  V+KSSKSI RVIPS+ LT R L NQ  GSLGSPSTSPLSSSS  IT+QQ+G G
Sbjct: 1   MFSSTFLVSKSSKSICRVIPSQHLTVRFLNNQLGGSLGSPSTSPLSSSS-SITTQQTGCG 60

Query: 117 GENRRSFISDFLIKSISSGILIVGVGSLIGSNNSSTANSHVSFADFPKQ-TW-AVREDQF 176
           GENRR+F+ +FLIK ISSG+LI GVGSL+ S+ SST+NSH+SFADFPK+ TW  V+EDQF
Sbjct: 61  GENRRNFVFEFLIKLISSGLLIGGVGSLL-SSKSSTSNSHLSFADFPKETTWTTVKEDQF 120

Query: 177 QYPSPNQ--NAKKKPKFLFGDDYRRKVFFNYEKRMRLQSPPEKVFEYFASIHTPEGEIFM 236
           QYPSPNQ  NAKKK KFLFGDDYRR+VFFNYEKR+R+QSPPEKVFEYFASIHTPEGEI+M
Sbjct: 121 QYPSPNQNINAKKKSKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTPEGEIYM 180

Query: 237 TPADLMRAIVPVFPPSESNRVREGFLRGERRVHGELYCVPSKFFMLFDTNNDGLISFAEY 296
            P+DLMRAIVPVFPPSESNRVREGFLRGERR+ GEL C PSKFFMLFDTNNDGLISFAEY
Sbjct: 181 RPSDLMRAIVPVFPPSESNRVREGFLRGERRISGELCCAPSKFFMLFDTNNDGLISFAEY 240

Query: 297 IFFVTLLSIPESSFSVAFKMFDIDNNGAIDREEFKKVMGLMRKQNRQGARHRDGRRLGMK 356
           IFFVTLLSIPESSFSVAFKMFDIDNNG IDREEFKKVMGLMRKQNRQGA HRDGRR GMK
Sbjct: 241 IFFVTLLSIPESSFSVAFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRFGMK 300

Query: 357 VCVENGGLVEYFFGQDGNDSLHHDKFVQFLRQLHEEILLLEFSHYDFKSQGSITAKDFAL 416
           V VENGGLVEYFFG+DG  SLHHDKFVQFLRQLHEEIL+LEFSHYDFKSQGSI+ KDFAL
Sbjct: 301 VSVENGGLVEYFFGEDGKGSLHHDKFVQFLRQLHEEILILEFSHYDFKSQGSISVKDFAL 360

Query: 417 SVVASADINHIDKLLDRVEALNKEPYFRNIRITYQEFKDFAELRKKLESFSLAIFSYGKV 476
           S+VASADINHIDKLLDRVEALNKEP+F+NIRITY+EFKDFAELRKKLESFSLAIFSYGKV
Sbjct: 361 SIVASADINHIDKLLDRVEALNKEPHFKNIRITYEEFKDFAELRKKLESFSLAIFSYGKV 420

Query: 477 NGALTKKDFQRAASHVSTKNFNLKAQFNKLNQPNNHMHVAFLCKVCGVSITRNVVDIIFH 536
           NG LTK+DFQRAAS                             +VCGVSIT+NVVDIIFH
Sbjct: 421 NGELTKQDFQRAAS-----------------------------QVCGVSITKNVVDIIFH 480

Query: 537 IFDVNADGDLSSDEFVRVVQRREVSSSQAAVGVAGILSCWFSCTAKCSSAKLFLRS 589
           IFD N DGDLSSDEFVRV+QRREV+SSQ AVGV G+LSCWFSC AKCSSAKLF+RS
Sbjct: 481 IFDANEDGDLSSDEFVRVIQRREVNSSQPAVGVGGLLSCWFSCAAKCSSAKLFVRS 505

BLAST of Spg003495 vs. NCBI nr
Match: XP_004139397.1 (calcium uptake protein, mitochondrial [Cucumis sativus] >KGN60757.1 hypothetical protein Csa_019233 [Cucumis sativus])

HSP 1 Score: 797.7 bits (2059), Expect = 6.6e-227
Identity = 429/541 (79.30%), Postives = 459/541 (84.84%), Query Frame = 0

Query: 57  MFSSTSSVAKSSKSISRVIPSRQLTARSLKNQPDGSLGSPSTSPL--SSSSLLITSQQSG 116
           MFSST  V+KSSK I RVIPS+ LT R L NQ  GSLGSPSTSPL  SSSS  ITSQQ+G
Sbjct: 1   MFSSTFLVSKSSKPICRVIPSQHLTVRFLDNQLGGSLGSPSTSPLFSSSSSSSITSQQTG 60

Query: 117 -SGGENRRSFISDFLIKSISSGILIVGVGSLIGSNNSSTANSHVSFADFPKQ-TW-AVRE 176
             GGENRRSF+ DFLIK ISSGILI GVGSL+ S+NSS +NSH+SFADFPK+ TW  V+E
Sbjct: 61  CGGGENRRSFVFDFLIKLISSGILIGGVGSLL-SSNSSASNSHLSFADFPKETTWTTVKE 120

Query: 177 DQFQYPS----PNQNAKKKPKFLFGDDYRRKVFFNYEKRMRLQSPPEKVFEYFASIHTPE 236
           DQFQYPS     N N KKK KFLFGDDYRR+VFFNYEKR+R+QSPPEKVFEYFASIHTPE
Sbjct: 121 DQFQYPSSPNHQNINPKKKSKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTPE 180

Query: 237 GEIFMTPADLMRAIVPVFPPSESNRVREGFLRGERRVHGELYCVPSKFFMLFDTNNDGLI 296
           GEI+M P+DLMRAIVPVFPPSESNRVREGFLRGERR+ GEL C PS FFMLFDTNNDGLI
Sbjct: 181 GEIYMRPSDLMRAIVPVFPPSESNRVREGFLRGERRMSGELCCAPSTFFMLFDTNNDGLI 240

Query: 297 SFAEYIFFVTLLSIPESSFSVAFKMFDIDNNGAIDREEFKKVMGLMRKQNRQGARHRDGR 356
           SFAEYIFFVTLLSIPESSFSVAFKMFDIDNNG IDREEFKKVMGLMRKQNRQGA HR+GR
Sbjct: 241 SFAEYIFFVTLLSIPESSFSVAFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHREGR 300

Query: 357 RLGMKVCVENGGLVEYFFGQDGNDSLHHDKFVQFLRQLHEEILLLEFSHYDFKSQGSITA 416
           R GMKV VENGGLVEYFFGQDG  SLHHDKFVQFLRQLH+EILLLEFSHYDFKSQGSI+A
Sbjct: 301 RFGMKVSVENGGLVEYFFGQDGKASLHHDKFVQFLRQLHDEILLLEFSHYDFKSQGSISA 360

Query: 417 KDFALSVVASADINHIDKLLDRVEALNKEPYFRNIRITYQEFKDFAELRKKLESFSLAIF 476
           KDFALS+VASADINHIDKLLDRVEALNKEP+F+NIRIT+ EFKDFAELRKKLESFSLAIF
Sbjct: 361 KDFALSIVASADINHIDKLLDRVEALNKEPHFKNIRITFDEFKDFAELRKKLESFSLAIF 420

Query: 477 SYGKVNGALTKKDFQRAASHVSTKNFNLKAQFNKLNQPNNHMHVAFLCKVCGVSITRNVV 536
           SYGKVNG LTK+DFQRAASH                             VCGVSIT+NVV
Sbjct: 421 SYGKVNGELTKQDFQRAASH-----------------------------VCGVSITKNVV 480

Query: 537 DIIFHIFDVNADGDLSSDEFVRVVQRREVSSSQAAVGVAGILSCWFSCTAKCSSAKLFLR 589
           DIIFHIFD N DGDLSSDEFVRV+QRREV+S+Q AVG  G+LSCWF C AKCS AKLF+R
Sbjct: 481 DIIFHIFDANEDGDLSSDEFVRVIQRREVNSTQPAVGAGGLLSCWFGCAAKCSYAKLFVR 511

BLAST of Spg003495 vs. NCBI nr
Match: XP_022143166.1 (calcium uptake protein, mitochondrial-like [Momordica charantia])

HSP 1 Score: 797.0 bits (2057), Expect = 1.1e-226
Identity = 422/533 (79.17%), Postives = 458/533 (85.93%), Query Frame = 0

Query: 57  MFSSTSSVAKSSKSISRVIPSRQLTARSLKNQPDGSLGSPSTSPLSSSSLLITSQQSGSG 116
           MFSST  V+KSS SI RV+ S++LT R   NQ     GS STSP  SSSLL +S  +GSG
Sbjct: 1   MFSSTLLVSKSSASIHRVVTSQRLTVRLFNNQLG---GSASTSP--SSSLLASSHNTGSG 60

Query: 117 GENRRSFISDFLIKSISSGILIVGVGSLIGSNNSSTANSHVSFADFPKQT-WAVREDQFQ 176
            E++RS I DFL+KS+SSG++ VG+GSL+   NSST+NS+VSFADF K+T W ++EDQFQ
Sbjct: 61  EESKRSSIFDFLVKSVSSGVVAVGIGSLL-CLNSSTSNSNVSFADFQKETKWTMKEDQFQ 120

Query: 177 YPSPNQNAKKKPKFLFGDDYRRKVFFNYEKRMRLQSPPEKVFEYFASIHTPEGEIFMTPA 236
           YP PNQNA+KK KFLFGDDYRR+VFFNYEKR+R+QSPPEKVFEYFAS+HTPEGEIFMTPA
Sbjct: 121 YPFPNQNARKKSKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASVHTPEGEIFMTPA 180

Query: 237 DLMRAIVPVFPPSESNRVREGFLRGERRVHGELYCVPSKFFMLFDTNNDGLISFAEYIFF 296
           DLMRAIVPVFPPSESNRVREGFLRGE R+HGELYCVPS+FFMLFD N+DGLISFAEYIFF
Sbjct: 181 DLMRAIVPVFPPSESNRVREGFLRGE-RIHGELYCVPSEFFMLFDINSDGLISFAEYIFF 240

Query: 297 VTLLSIPESSFSVAFKMFDIDNNGAIDREEFKKVMGLMRKQNRQGARHRDGRRLGMKVCV 356
           VTLLSIPESSFSVAFKMFDIDNNG IDREEFKKVM LMRKQNRQGARHRDGRRLGMKVCV
Sbjct: 241 VTLLSIPESSFSVAFKMFDIDNNGEIDREEFKKVMALMRKQNRQGARHRDGRRLGMKVCV 300

Query: 357 ENGGLVEYFFGQDGNDSLHHDKFVQFLRQLHEEILLLEFSHYDFKSQGSITAKDFALSVV 416
           ENGGLVEYFFGQDG  SLHHDKFVQFLRQLH+EIL LEFSHYDFKSQGSI+AKDFALS+V
Sbjct: 301 ENGGLVEYFFGQDGKGSLHHDKFVQFLRQLHDEILRLEFSHYDFKSQGSISAKDFALSMV 360

Query: 417 ASADINHIDKLLDRVEALNKEPYFRNIRITYQEFKDFAELRKKLESFSLAIFSYGKVNGA 476
           ASADINHIDKLLDRVEALNKEP FRNIRITY+EFKDFAELRKKLESFSLAIFSYGKVNG 
Sbjct: 361 ASADINHIDKLLDRVEALNKEPQFRNIRITYEEFKDFAELRKKLESFSLAIFSYGKVNGE 420

Query: 477 LTKKDFQRAASHVSTKNFNLKAQFNKLNQPNNHMHVAFLCKVCGVSITRNVVDIIFHIFD 536
           LTK+DFQRAASH                             VCGVSITRNVVDIIFHIFD
Sbjct: 421 LTKQDFQRAASH-----------------------------VCGVSITRNVVDIIFHIFD 480

Query: 537 VNADGDLSSDEFVRVVQRREVSSSQAAVGVAGILSCWFSCTAKCSSAKLFLRS 589
            N DGDLSSDEFVRV+QR+EVSSSQAAVG  G LSCWFSC+AKCSSAKLF+RS
Sbjct: 481 ANGDGDLSSDEFVRVIQRKEVSSSQAAVGAGGFLSCWFSCSAKCSSAKLFVRS 497

BLAST of Spg003495 vs. ExPASy Swiss-Prot
Match: Q9SZ45 (Calcium uptake protein, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=MICU PE=2 SV=1)

HSP 1 Score: 463.8 bits (1192), Expect = 3.0e-129
Identity = 242/397 (60.96%), Postives = 299/397 (75.31%), Query Frame = 0

Query: 189 FLFGDDYRRKVFFNYEKRMRLQSPPEKVFEYFASIHTPEGEIFMTPADLMRAIVPVFPPS 248
           F+FGD YRRK+FFNYEKR+RLQSPPEKVFEYFAS+ T +GEI M PADLMRAIVPVFPPS
Sbjct: 132 FIFGDAYRRKIFFNYEKRLRLQSPPEKVFEYFASVRTDKGEILMKPADLMRAIVPVFPPS 191

Query: 249 ESNRVREGFLRGERRVHGELYCVPSKFFMLFDTNNDGLISFAEYIFFVTLLSIPESSFSV 308
           ES+ VREG+L GER   GEL C PS+FFMLFD +NDGLISF EYIFFVTLLSIPESSF+V
Sbjct: 192 ESHLVREGYLTGERN-PGELRCSPSEFFMLFDVDNDGLISFKEYIFFVTLLSIPESSFAV 251

Query: 309 AFKMFDIDNNGAIDREEFKKVMGLMRKQNRQGARHRDGRRLGMKV--CVENGGLVEYFFG 368
           AFKMFD DNNG ID+EEFK VM LMR Q+RQG  HRDG R G+ +   VE+GGLVEYFFG
Sbjct: 252 AFKMFDTDNNGEIDKEEFKTVMSLMRSQHRQGVGHRDGLRTGLHMTGSVEDGGLVEYFFG 311

Query: 369 QDGNDSLHHDKFVQFLRQLHEEILLLEFSHYDFKSQGSITAKDFALSVVASADINHIDKL 428
           +DG+  L HDKF +F++ L EE+L LEF+HYD+K +GSI+AKDFALS+VA+AD +H+ KL
Sbjct: 312 KDGSQKLKHDKFTKFMKDLTEEMLRLEFAHYDYKRRGSISAKDFALSMVAAADASHLSKL 371

Query: 429 LDRVEALNKEPYFRNIRITYQEFKDFAELRKKLESFSLAIFSYGKVNGALTKKDFQRAAS 488
           LDRVE+L++ P+ R++RI+ +EFK F ELR KL  FSLA+F+YGK NG LT KDF+RAAS
Sbjct: 372 LDRVESLSEHPHLRDMRISLKEFKQFDELRSKLGPFSLALFAYGKANGLLTMKDFKRAAS 431

Query: 489 HVSTKNFNLKAQFNKLNQPNNHMHVAFLCKVCGVSITRNVVDIIFHIFDVNADGDLSSDE 548
                                        +VCG++++ NV++I FH+FD N DG+LS DE
Sbjct: 432 -----------------------------QVCGITLSDNVIEIAFHVFDSNQDGNLSVDE 491

Query: 549 FVRVVQRREVSSSQ-AAVGVAGILSCWFSCTAKCSSA 583
           F+RV+ RRE   +Q  A G++   S  +  +  CSS+
Sbjct: 492 FLRVLHRRERDVAQPIAKGLSRYFSDGWKGSKNCSSS 498

BLAST of Spg003495 vs. ExPASy Swiss-Prot
Match: Q95PZ2 (Calcium uptake protein 1 homolog, mitochondrial OS=Caenorhabditis elegans OX=6239 GN=micu-1 PE=2 SV=2)

HSP 1 Score: 167.9 bits (424), Expect = 3.4e-40
Identity = 124/387 (32.04%), Postives = 186/387 (48.06%), Query Frame = 0

Query: 181 QNAKKKPKFLFGDDYRRKVFFNYEKRMRLQSPPEKVFEYFASI-------HTPEGEIFMT 240
           +  K+KPK      +R +    YE R+RL S P+K+F YFA++        +   E+FMT
Sbjct: 147 EEVKEKPK-KKKLGFRERRIIEYEDRLRLYSTPDKIFRYFATLKIIDPNEDSGRFEVFMT 206

Query: 241 PADLMRAIVP-VFPPSESNRVREGFLRGERRVHGELYCVPSKFFMLFDTNNDGLISFAEY 300
           P D +R+  P V  P             E+    +     S F+ L     +GLI+F++Y
Sbjct: 207 PEDFLRSFTPGVMQPRRWGLDSFKNYNPEKHKRHKFSDPDSIFYKL---GENGLINFSDY 266

Query: 301 IFFVTLLSIPESSFSVAFKMFDIDNNGAIDREEFKKVMGLMRKQNRQGARHRDGRRLGMK 360
           +F +TLLS   + F++AFK+FD+D NGA+D+EEF KV  L+  Q   G RHRD       
Sbjct: 267 LFLMTLLSTSHADFALAFKIFDVDGNGALDKEEFTKVQQLIMSQTTVGQRHRDHITPNQS 326

Query: 361 VCVE-NGGLVEYFFGQDGNDSLHHDKFVQFLRQLHEEILLLEFSHYDF--KSQGSITAKD 420
             VE N  L  YFFG+DG  SL  +KF++F  +L  +IL +EF   D      G I    
Sbjct: 327 FRVETNSALETYFFGKDGKGSLSSEKFIEFQERLQHDILKMEFERRDALDNPDGLINEDS 386

Query: 421 FALSVVASADINHIDK--LLDRVEALNKEPYFRNIRITYQEFKDFAELRKKLESFSLAIF 480
           FA  ++  A IN   +  +L RV+   K    +   I++ E K F E    ++   +A+ 
Sbjct: 387 FAQLLLLHAQINEKKQKHMLKRVKRRFKGENLKG--ISFGETKAFFEFLYHIDDVDIALH 446

Query: 481 SYGKVNGALTKKDFQRAASHVSTKNFNLKAQFNKLNQPNNHMHVAFLCKVCGVSITRNVV 540
            +     ++  K  QR A                              KV G+ ++ +VV
Sbjct: 447 FHKMAGMSIDAKLLQRVA-----------------------------VKVTGIPLSDHVV 498

Query: 541 DIIFHIFDVNADGDLSSDEFVRVVQRR 555
           D++  +FD N DG LS +E V V++RR
Sbjct: 507 DVVITLFDDNLDGKLSHEEMVAVMRRR 498

BLAST of Spg003495 vs. ExPASy Swiss-Prot
Match: Q4R518 (Calcium uptake protein 1, mitochondrial OS=Macaca fascicularis OX=9541 GN=MICU1 PE=2 SV=2)

HSP 1 Score: 167.9 bits (424), Expect = 3.4e-40
Identity = 118/387 (30.49%), Postives = 192/387 (49.61%), Query Frame = 0

Query: 178 SPNQNAKKKPKFLFGDDYRRKVFFNYEKRMRLQSPPEKVFEYFAS---IHTP-EGEIFMT 237
           +P+   KKK +  F D   RKV   YE R+R  S P+K+F YFA+   I+ P E E+FMT
Sbjct: 93  APHPEEKKKKRSGFRD---RKV-MEYENRIRAYSTPDKIFRYFATLKVINEPGEAEVFMT 152

Query: 238 PADLMRAIVPVFPPSESNRVREGFLR--GERRVHGELYCVPSKFFMLFDTNNDGLISFAE 297
           P D +R+I P     E   + +  ++    +++  E      +  + +     GLISF++
Sbjct: 153 PEDFVRSITPNEKQPEHLGLDQYIIKRFDGKKISQEREKFADEGSIFYTLGECGLISFSD 212

Query: 298 YIFFVTLLSIPESSFSVAFKMFDIDNNGAIDREEFKKVMGLMRKQNRQGARHRD----GR 357
           YIF  T+LS P+ +F +AFKMFD++ +G +D EEF++V  ++R Q   G RHRD    G 
Sbjct: 213 YIFLTTVLSTPQRNFEIAFKMFDLNGDGEVDMEEFEQVQSIIRSQTSMGMRHRDRPTTGN 272

Query: 358 RLGMKVCVENGGLVEYFFGQDGNDSLHHDKFVQFLRQLHEEILLLEFSHYDFKSQGSITA 417
            L   +C     L  YFFG D    L    F++F R+L  ++L LEF  +D    G IT 
Sbjct: 273 TLKSGLC---SALTTYFFGADLKGKLTIKNFLEFQRKLQHDVLKLEFERHD-PVDGRITE 332

Query: 418 KDFALSVVASADINHIDKLLDRVEALNKEPYFRNIRITYQEFKDFAELRKKLESFSLAIF 477
           + F   ++A + +    K L  ++   K+ +     +T+QE ++F    K +     A+ 
Sbjct: 333 RQFGGMLLAYSGVQ--SKKLTAMQRQLKKHFKEGKGLTFQEVENFFTFLKNINDVDTALS 392

Query: 478 SYGKVNGALTKKDFQRAASHVSTKNFNLKAQFNKLNQPNNHMHVAFLCKVCGVSITRNVV 537
            Y     +L K   Q+ A  V+                              V ++ +V 
Sbjct: 393 FYHMAGASLDKVTMQQVARTVAK-----------------------------VELSDHVC 440

Query: 538 DIIFHIFDVNADGDLSSDEFVRVVQRR 555
           D++F +FD + +G+LS+ EFV ++++R
Sbjct: 453 DVVFALFDCDGNGELSNKEFVSIMKQR 440

BLAST of Spg003495 vs. ExPASy Swiss-Prot
Match: Q9BPX6 (Calcium uptake protein 1, mitochondrial OS=Homo sapiens OX=9606 GN=MICU1 PE=1 SV=1)

HSP 1 Score: 167.5 bits (423), Expect = 4.4e-40
Identity = 116/387 (29.97%), Postives = 190/387 (49.10%), Query Frame = 0

Query: 178 SPNQNAKKKPKFLFGDDYRRKVFFNYEKRMRLQSPPEKVFEYFASI----HTPEGEIFMT 237
           +P+   KKK +  F D   RKV   YE R+R  S P+K+F YFA++       E E+FMT
Sbjct: 93  APHPEEKKKKRSGFRD---RKV-MEYENRIRAYSTPDKIFRYFATLKVISEPGEAEVFMT 152

Query: 238 PADLMRAIVPVFPPSESNRVREGFLR--GERRVHGELYCVPSKFFMLFDTNNDGLISFAE 297
           P D +R+I P     E   + +  ++    +++  E      +  + +     GLISF++
Sbjct: 153 PEDFVRSITPNEKQPEHLGLDQYIIKRFDGKKISQEREKFADEGSIFYTLGECGLISFSD 212

Query: 298 YIFFVTLLSIPESSFSVAFKMFDIDNNGAIDREEFKKVMGLMRKQNRQGARHRD----GR 357
           YIF  T+LS P+ +F +AFKMFD++ +G +D EEF++V  ++R Q   G RHRD    G 
Sbjct: 213 YIFLTTVLSTPQRNFEIAFKMFDLNGDGEVDMEEFEQVQSIIRSQTSMGMRHRDRPTTGN 272

Query: 358 RLGMKVCVENGGLVEYFFGQDGNDSLHHDKFVQFLRQLHEEILLLEFSHYDFKSQGSITA 417
            L   +C     L  YFFG D    L    F++F R+L  ++L LEF  +D    G IT 
Sbjct: 273 TLKSGLC---SALTTYFFGADLKGKLTIKNFLEFQRKLQHDVLKLEFERHD-PVDGRITE 332

Query: 418 KDFALSVVASADINHIDKLLDRVEALNKEPYFRNIRITYQEFKDFAELRKKLESFSLAIF 477
           + F   ++A + +    K L  ++   K+ +     +T+QE ++F    K +     A+ 
Sbjct: 333 RQFGGMLLAYSGVQ--SKKLTAMQRQLKKHFKEGKGLTFQEVENFFTFLKNINDVDTALS 392

Query: 478 SYGKVNGALTKKDFQRAASHVSTKNFNLKAQFNKLNQPNNHMHVAFLCKVCGVSITRNVV 537
            Y     +L K   Q+ A  V+                              V ++ +V 
Sbjct: 393 FYHMAGASLDKVTMQQVARTVAK-----------------------------VELSDHVC 440

Query: 538 DIIFHIFDVNADGDLSSDEFVRVVQRR 555
           D++F +FD + +G+LS+ EFV ++++R
Sbjct: 453 DVVFALFDCDGNGELSNKEFVSIMKQR 440

BLAST of Spg003495 vs. ExPASy Swiss-Prot
Match: B1H2N3 (Calcium uptake protein 1, mitochondrial OS=Xenopus tropicalis OX=8364 GN=micu1 PE=2 SV=2)

HSP 1 Score: 167.5 bits (423), Expect = 4.4e-40
Identity = 114/384 (29.69%), Postives = 192/384 (50.00%), Query Frame = 0

Query: 179 PNQNAKKKPKFLFGDDYRRKVFFNYEKRMRLQSPPEKVFEYFASI----HTPEGEIFMTP 238
           P +  KKK +  F D   RKV   YE R+R  S P+K+F YFA++     + E E+FMTP
Sbjct: 91  PQEGKKKKARVGFRD---RKV-MEYENRIRAYSTPDKIFRYFATLKVIHESGESEVFMTP 150

Query: 239 ADLMRAIVPVFPPSESNRVREGFLR--GERRVHGELYCVPSKFFMLFDTNNDGLISFAEY 298
            D +R+I P     E+  + +  ++    +++  E      +  + +     GLISF++Y
Sbjct: 151 QDFVRSITPNEKQPENLGLDQFIIKRYDGKKISQEREKFADEDSIFYSLGECGLISFSDY 210

Query: 299 IFFVTLLSIPESSFSVAFKMFDIDNNGAIDREEFKKVMGLMRKQNRQGARHRDGRRLGMK 358
           IF  T+LS P+ +F +AFKMFD++ +G +D EEF++V  ++R Q   G RHRD    G  
Sbjct: 211 IFLTTVLSTPQRNFEIAFKMFDLNGDGEVDMEEFEQVQSIIRSQTSMGMRHRDRSTTGNT 270

Query: 359 VCVE-NGGLVEYFFGQDGNDSLHHDKFVQFLRQLHEEILLLEFSHYDFKSQGSITAKDFA 418
           +    +  L  YFFG D    L    F++F R+L  ++L LEF  +D    G IT + F 
Sbjct: 271 LKTGFSSALTTYFFGADLKGKLTIKNFLEFQRKLQHDVLKLEFERHD-PVDGHITERQFG 330

Query: 419 LSVVASADINHIDKLLDRVEALNKEPYFRNIR-ITYQEFKDFAELRKKLESFSLAIFSYG 478
             ++A + +    KL   ++ L K   F++   +T++E ++F    K +     A+  Y 
Sbjct: 331 SMLLAYSGVQS-KKLTHMLKQLKKR--FKDAEGLTFEEVENFFTFLKNINDVDTALSFYH 390

Query: 479 KVNGALTKKDFQRAASHVSTKNFNLKAQFNKLNQPNNHMHVAFLCKVCGVSITRNVVDII 538
               +L K   Q+ A  V+                              V ++ +V D++
Sbjct: 391 MAGASLDKVTMQQVARTVAK-----------------------------VELSDHVCDVV 437

Query: 539 FHIFDVNADGDLSSDEFVRVVQRR 555
           F +FD + +G+LS+ EF+ ++++R
Sbjct: 451 FALFDCDGNGELSNKEFIAIMKQR 437

BLAST of Spg003495 vs. ExPASy TrEMBL
Match: A0A1S3C3I8 (calcium uptake protein 1, mitochondrial-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103496476 PE=4 SV=1)

HSP 1 Score: 836.3 bits (2159), Expect = 8.1e-239
Identity = 444/537 (82.68%), Postives = 479/537 (89.20%), Query Frame = 0

Query: 57  MFSSTSSVAKSSKSISRVIPSRQLTARSLKNQPDGSLGSPSTSPLSSSSLLITSQQSGSG 116
           MFSST  V+KSSKSI RVIPS+ LT R L NQ  GSLGSPSTSPLSSSS  IT+QQ+G G
Sbjct: 1   MFSSTFLVSKSSKSICRVIPSQHLTVRFLNNQLGGSLGSPSTSPLSSSS-SITTQQTGCG 60

Query: 117 GENRRSFISDFLIKSISSGILIVGVGSLIGSNNSSTANSHVSFADFPKQ-TW-AVREDQF 176
           GENRR+F+ +FLIK ISSG+LI GVGSL+ S+ SST+NSH+SFADFPK+ TW  V+EDQF
Sbjct: 61  GENRRNFVFEFLIKLISSGLLIGGVGSLL-SSKSSTSNSHLSFADFPKETTWTTVKEDQF 120

Query: 177 QYPSPNQ--NAKKKPKFLFGDDYRRKVFFNYEKRMRLQSPPEKVFEYFASIHTPEGEIFM 236
           QYPSPNQ  NAKKK KFLFGDDYRR+VFFNYEKR+R+QSPPEKVFEYFASIHTPEGEI+M
Sbjct: 121 QYPSPNQNINAKKKSKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTPEGEIYM 180

Query: 237 TPADLMRAIVPVFPPSESNRVREGFLRGERRVHGELYCVPSKFFMLFDTNNDGLISFAEY 296
            P+DLMRAIVPVFPPSESNRVREGFLRGERR+ GEL C PSKFFMLFDTNNDGLISFAEY
Sbjct: 181 RPSDLMRAIVPVFPPSESNRVREGFLRGERRISGELCCAPSKFFMLFDTNNDGLISFAEY 240

Query: 297 IFFVTLLSIPESSFSVAFKMFDIDNNGAIDREEFKKVMGLMRKQNRQGARHRDGRRLGMK 356
           IFFVTLLSIPESSFSVAFKMFDIDNNG IDREEFKKVMGLMRKQNRQGA HRDGRR GMK
Sbjct: 241 IFFVTLLSIPESSFSVAFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRFGMK 300

Query: 357 VCVENGGLVEYFFGQDGNDSLHHDKFVQFLRQLHEEILLLEFSHYDFKSQGSITAKDFAL 416
           V VENGGLVEYFFG+DG  SLHHDKFVQFLRQLHEEIL+LEFSHYDFKSQGSI+ KDFAL
Sbjct: 301 VSVENGGLVEYFFGEDGKGSLHHDKFVQFLRQLHEEILILEFSHYDFKSQGSISVKDFAL 360

Query: 417 SVVASADINHIDKLLDRVEALNKEPYFRNIRITYQEFKDFAELRKKLESFSLAIFSYGKV 476
           S+VASADINHIDKLLDRVEALNKEP+F+NIRITY+EFKDFAELRKKLESFSLAIFSYGKV
Sbjct: 361 SIVASADINHIDKLLDRVEALNKEPHFKNIRITYEEFKDFAELRKKLESFSLAIFSYGKV 420

Query: 477 NGALTKKDFQRAASHVSTKNFNLKAQFNKLNQPNNH-MHVAFLCKVCGVSITRNVVDIIF 536
           NG LTK+DFQRAAS VST   +   Q NKL     H +   FL KVCGVSIT+NVVDIIF
Sbjct: 421 NGELTKQDFQRAASQVST---HQTFQVNKLKINLIHNVGFVFLYKVCGVSITKNVVDIIF 480

Query: 537 HIFDVNADGDLSSDEFVRVVQRREVSSSQAAVGVAGILSCWFSCTAKCSSAKLFLRS 589
           HIFD N DGDLSSDEFVRV+QRREV+SSQ AVGV G+LSCWFSC AKCSSAKLF+RS
Sbjct: 481 HIFDANEDGDLSSDEFVRVIQRREVNSSQPAVGVGGLLSCWFSCAAKCSSAKLFVRS 532

BLAST of Spg003495 vs. ExPASy TrEMBL
Match: A0A5A7VDG3 (Calcium uptake protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold285G001000 PE=4 SV=1)

HSP 1 Score: 819.7 bits (2116), Expect = 7.8e-234
Identity = 434/536 (80.97%), Postives = 468/536 (87.31%), Query Frame = 0

Query: 57  MFSSTSSVAKSSKSISRVIPSRQLTARSLKNQPDGSLGSPSTSPLSSSSLLITSQQSGSG 116
           MFSST  V+KSSKSI RVIPS+ LT R L NQ  GSLGSPSTSPLSSSS  IT+QQ+G G
Sbjct: 1   MFSSTFLVSKSSKSICRVIPSQHLTVRFLNNQLGGSLGSPSTSPLSSSS-SITTQQTGCG 60

Query: 117 GENRRSFISDFLIKSISSGILIVGVGSLIGSNNSSTANSHVSFADFPKQ-TW-AVREDQF 176
           GENRR+F+ +FLIK ISSGILI GVGSL+ S+NSST+NSH+SFA+FPK+ TW  V+EDQF
Sbjct: 61  GENRRNFVFEFLIKLISSGILIGGVGSLL-SSNSSTSNSHLSFAEFPKETTWTTVKEDQF 120

Query: 177 QYPSPNQ--NAKKKPKFLFGDDYRRKVFFNYEKRMRLQSPPEKVFEYFASIHTPEGEIFM 236
           QYPSPNQ  NAKKK KFLFGDDYRR+VFFNYEKR+R+QSPPEKVFEYFASIHTPEGEI+M
Sbjct: 121 QYPSPNQNINAKKKSKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTPEGEIYM 180

Query: 237 TPADLMRAIVPVFPPSESNRVREGFLRGERRVHGELYCVPSKFFMLFDTNNDGLISFAEY 296
            P+DLMRAIVPVFPPSESNRVREGFLRGERR+ GEL C PSKFFMLFDTNNDGLISFAEY
Sbjct: 181 RPSDLMRAIVPVFPPSESNRVREGFLRGERRISGELCCAPSKFFMLFDTNNDGLISFAEY 240

Query: 297 IFFVTLLSIPESSFSVAFKMFDIDNNGAIDREEFKKVMGLMRKQNRQGARHRDGRRLGMK 356
           IFFVTLLSIPESSFSVAFKMFDIDNNG IDREEFKKVMGLMRKQNRQGA HRDGRR GMK
Sbjct: 241 IFFVTLLSIPESSFSVAFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRFGMK 300

Query: 357 VCVENGGLVEYFFGQDGNDSLHHDKFVQFLRQLHEEILLLEFSHYDFKSQGSITAKDFAL 416
           V VENGGLVEYFFG+DG  SLHHDKFVQFLRQLHEEIL+LEFSHYDFKSQGSI+ KDFAL
Sbjct: 301 VSVENGGLVEYFFGEDGKGSLHHDKFVQFLRQLHEEILILEFSHYDFKSQGSISVKDFAL 360

Query: 417 SVVASADINHIDKLLDRVEALNKEPYFRNIRITYQEFKDFAELRKKLESFSLAIFSYGKV 476
           S+VASADINHIDKLLDRVEALNKEP+F+NIRITY+EFKDFAELRKKLESFSLAIFSYGKV
Sbjct: 361 SIVASADINHIDKLLDRVEALNKEPHFKNIRITYEEFKDFAELRKKLESFSLAIFSYGKV 420

Query: 477 NGALTKKDFQRAASHVSTKNFNLKAQFNKLNQPNNHMHVAFLCKVCGVSITRNVVDIIFH 536
           NG LTK+DFQRAAS                             +VCGVSIT+NVVDIIFH
Sbjct: 421 NGELTKQDFQRAAS-----------------------------QVCGVSITKNVVDIIFH 480

Query: 537 IFDVNADGDLSSDEFVRVVQRREVSSSQAAVGVAGILSCWFSCTAKCSSAKLFLRS 589
           IFD N DGDLSSDEFVRV+QRREV+SSQ AVGV G+LSCWFSC AKCSSAKLF+RS
Sbjct: 481 IFDANEDGDLSSDEFVRVIQRREVNSSQPAVGVGGLLSCWFSCAAKCSSAKLFVRS 505

BLAST of Spg003495 vs. ExPASy TrEMBL
Match: A0A1S3C4U2 (calcium uptake protein 1, mitochondrial-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103496476 PE=4 SV=1)

HSP 1 Score: 818.1 bits (2112), Expect = 2.3e-233
Identity = 433/536 (80.78%), Postives = 467/536 (87.13%), Query Frame = 0

Query: 57  MFSSTSSVAKSSKSISRVIPSRQLTARSLKNQPDGSLGSPSTSPLSSSSLLITSQQSGSG 116
           MFSST  V+KSSKSI RVIPS+ LT R L NQ  GSLGSPSTSPLSSSS  IT+QQ+G G
Sbjct: 1   MFSSTFLVSKSSKSICRVIPSQHLTVRFLNNQLGGSLGSPSTSPLSSSS-SITTQQTGCG 60

Query: 117 GENRRSFISDFLIKSISSGILIVGVGSLIGSNNSSTANSHVSFADFPKQ-TW-AVREDQF 176
           GENRR+F+ +FLIK ISSG+LI GVGSL+ S+ SST+NSH+SFADFPK+ TW  V+EDQF
Sbjct: 61  GENRRNFVFEFLIKLISSGLLIGGVGSLL-SSKSSTSNSHLSFADFPKETTWTTVKEDQF 120

Query: 177 QYPSPNQ--NAKKKPKFLFGDDYRRKVFFNYEKRMRLQSPPEKVFEYFASIHTPEGEIFM 236
           QYPSPNQ  NAKKK KFLFGDDYRR+VFFNYEKR+R+QSPPEKVFEYFASIHTPEGEI+M
Sbjct: 121 QYPSPNQNINAKKKSKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTPEGEIYM 180

Query: 237 TPADLMRAIVPVFPPSESNRVREGFLRGERRVHGELYCVPSKFFMLFDTNNDGLISFAEY 296
            P+DLMRAIVPVFPPSESNRVREGFLRGERR+ GEL C PSKFFMLFDTNNDGLISFAEY
Sbjct: 181 RPSDLMRAIVPVFPPSESNRVREGFLRGERRISGELCCAPSKFFMLFDTNNDGLISFAEY 240

Query: 297 IFFVTLLSIPESSFSVAFKMFDIDNNGAIDREEFKKVMGLMRKQNRQGARHRDGRRLGMK 356
           IFFVTLLSIPESSFSVAFKMFDIDNNG IDREEFKKVMGLMRKQNRQGA HRDGRR GMK
Sbjct: 241 IFFVTLLSIPESSFSVAFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRFGMK 300

Query: 357 VCVENGGLVEYFFGQDGNDSLHHDKFVQFLRQLHEEILLLEFSHYDFKSQGSITAKDFAL 416
           V VENGGLVEYFFG+DG  SLHHDKFVQFLRQLHEEIL+LEFSHYDFKSQGSI+ KDFAL
Sbjct: 301 VSVENGGLVEYFFGEDGKGSLHHDKFVQFLRQLHEEILILEFSHYDFKSQGSISVKDFAL 360

Query: 417 SVVASADINHIDKLLDRVEALNKEPYFRNIRITYQEFKDFAELRKKLESFSLAIFSYGKV 476
           S+VASADINHIDKLLDRVEALNKEP+F+NIRITY+EFKDFAELRKKLESFSLAIFSYGKV
Sbjct: 361 SIVASADINHIDKLLDRVEALNKEPHFKNIRITYEEFKDFAELRKKLESFSLAIFSYGKV 420

Query: 477 NGALTKKDFQRAASHVSTKNFNLKAQFNKLNQPNNHMHVAFLCKVCGVSITRNVVDIIFH 536
           NG LTK+DFQRAAS                             +VCGVSIT+NVVDIIFH
Sbjct: 421 NGELTKQDFQRAAS-----------------------------QVCGVSITKNVVDIIFH 480

Query: 537 IFDVNADGDLSSDEFVRVVQRREVSSSQAAVGVAGILSCWFSCTAKCSSAKLFLRS 589
           IFD N DGDLSSDEFVRV+QRREV+SSQ AVGV G+LSCWFSC AKCSSAKLF+RS
Sbjct: 481 IFDANEDGDLSSDEFVRVIQRREVNSSQPAVGVGGLLSCWFSCAAKCSSAKLFVRS 505

BLAST of Spg003495 vs. ExPASy TrEMBL
Match: A0A0A0LJ54 (Calcium ion binding protein OS=Cucumis sativus OX=3659 GN=Csa_2G009450 PE=4 SV=1)

HSP 1 Score: 797.7 bits (2059), Expect = 3.2e-227
Identity = 429/541 (79.30%), Postives = 459/541 (84.84%), Query Frame = 0

Query: 57  MFSSTSSVAKSSKSISRVIPSRQLTARSLKNQPDGSLGSPSTSPL--SSSSLLITSQQSG 116
           MFSST  V+KSSK I RVIPS+ LT R L NQ  GSLGSPSTSPL  SSSS  ITSQQ+G
Sbjct: 1   MFSSTFLVSKSSKPICRVIPSQHLTVRFLDNQLGGSLGSPSTSPLFSSSSSSSITSQQTG 60

Query: 117 -SGGENRRSFISDFLIKSISSGILIVGVGSLIGSNNSSTANSHVSFADFPKQ-TW-AVRE 176
             GGENRRSF+ DFLIK ISSGILI GVGSL+ S+NSS +NSH+SFADFPK+ TW  V+E
Sbjct: 61  CGGGENRRSFVFDFLIKLISSGILIGGVGSLL-SSNSSASNSHLSFADFPKETTWTTVKE 120

Query: 177 DQFQYPS----PNQNAKKKPKFLFGDDYRRKVFFNYEKRMRLQSPPEKVFEYFASIHTPE 236
           DQFQYPS     N N KKK KFLFGDDYRR+VFFNYEKR+R+QSPPEKVFEYFASIHTPE
Sbjct: 121 DQFQYPSSPNHQNINPKKKSKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTPE 180

Query: 237 GEIFMTPADLMRAIVPVFPPSESNRVREGFLRGERRVHGELYCVPSKFFMLFDTNNDGLI 296
           GEI+M P+DLMRAIVPVFPPSESNRVREGFLRGERR+ GEL C PS FFMLFDTNNDGLI
Sbjct: 181 GEIYMRPSDLMRAIVPVFPPSESNRVREGFLRGERRMSGELCCAPSTFFMLFDTNNDGLI 240

Query: 297 SFAEYIFFVTLLSIPESSFSVAFKMFDIDNNGAIDREEFKKVMGLMRKQNRQGARHRDGR 356
           SFAEYIFFVTLLSIPESSFSVAFKMFDIDNNG IDREEFKKVMGLMRKQNRQGA HR+GR
Sbjct: 241 SFAEYIFFVTLLSIPESSFSVAFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHREGR 300

Query: 357 RLGMKVCVENGGLVEYFFGQDGNDSLHHDKFVQFLRQLHEEILLLEFSHYDFKSQGSITA 416
           R GMKV VENGGLVEYFFGQDG  SLHHDKFVQFLRQLH+EILLLEFSHYDFKSQGSI+A
Sbjct: 301 RFGMKVSVENGGLVEYFFGQDGKASLHHDKFVQFLRQLHDEILLLEFSHYDFKSQGSISA 360

Query: 417 KDFALSVVASADINHIDKLLDRVEALNKEPYFRNIRITYQEFKDFAELRKKLESFSLAIF 476
           KDFALS+VASADINHIDKLLDRVEALNKEP+F+NIRIT+ EFKDFAELRKKLESFSLAIF
Sbjct: 361 KDFALSIVASADINHIDKLLDRVEALNKEPHFKNIRITFDEFKDFAELRKKLESFSLAIF 420

Query: 477 SYGKVNGALTKKDFQRAASHVSTKNFNLKAQFNKLNQPNNHMHVAFLCKVCGVSITRNVV 536
           SYGKVNG LTK+DFQRAASH                             VCGVSIT+NVV
Sbjct: 421 SYGKVNGELTKQDFQRAASH-----------------------------VCGVSITKNVV 480

Query: 537 DIIFHIFDVNADGDLSSDEFVRVVQRREVSSSQAAVGVAGILSCWFSCTAKCSSAKLFLR 589
           DIIFHIFD N DGDLSSDEFVRV+QRREV+S+Q AVG  G+LSCWF C AKCS AKLF+R
Sbjct: 481 DIIFHIFDANEDGDLSSDEFVRVIQRREVNSTQPAVGAGGLLSCWFGCAAKCSYAKLFVR 511

BLAST of Spg003495 vs. ExPASy TrEMBL
Match: A0A6J1CPF9 (calcium uptake protein, mitochondrial-like OS=Momordica charantia OX=3673 GN=LOC111013100 PE=4 SV=1)

HSP 1 Score: 797.0 bits (2057), Expect = 5.4e-227
Identity = 422/533 (79.17%), Postives = 458/533 (85.93%), Query Frame = 0

Query: 57  MFSSTSSVAKSSKSISRVIPSRQLTARSLKNQPDGSLGSPSTSPLSSSSLLITSQQSGSG 116
           MFSST  V+KSS SI RV+ S++LT R   NQ     GS STSP  SSSLL +S  +GSG
Sbjct: 1   MFSSTLLVSKSSASIHRVVTSQRLTVRLFNNQLG---GSASTSP--SSSLLASSHNTGSG 60

Query: 117 GENRRSFISDFLIKSISSGILIVGVGSLIGSNNSSTANSHVSFADFPKQT-WAVREDQFQ 176
            E++RS I DFL+KS+SSG++ VG+GSL+   NSST+NS+VSFADF K+T W ++EDQFQ
Sbjct: 61  EESKRSSIFDFLVKSVSSGVVAVGIGSLL-CLNSSTSNSNVSFADFQKETKWTMKEDQFQ 120

Query: 177 YPSPNQNAKKKPKFLFGDDYRRKVFFNYEKRMRLQSPPEKVFEYFASIHTPEGEIFMTPA 236
           YP PNQNA+KK KFLFGDDYRR+VFFNYEKR+R+QSPPEKVFEYFAS+HTPEGEIFMTPA
Sbjct: 121 YPFPNQNARKKSKFLFGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASVHTPEGEIFMTPA 180

Query: 237 DLMRAIVPVFPPSESNRVREGFLRGERRVHGELYCVPSKFFMLFDTNNDGLISFAEYIFF 296
           DLMRAIVPVFPPSESNRVREGFLRGE R+HGELYCVPS+FFMLFD N+DGLISFAEYIFF
Sbjct: 181 DLMRAIVPVFPPSESNRVREGFLRGE-RIHGELYCVPSEFFMLFDINSDGLISFAEYIFF 240

Query: 297 VTLLSIPESSFSVAFKMFDIDNNGAIDREEFKKVMGLMRKQNRQGARHRDGRRLGMKVCV 356
           VTLLSIPESSFSVAFKMFDIDNNG IDREEFKKVM LMRKQNRQGARHRDGRRLGMKVCV
Sbjct: 241 VTLLSIPESSFSVAFKMFDIDNNGEIDREEFKKVMALMRKQNRQGARHRDGRRLGMKVCV 300

Query: 357 ENGGLVEYFFGQDGNDSLHHDKFVQFLRQLHEEILLLEFSHYDFKSQGSITAKDFALSVV 416
           ENGGLVEYFFGQDG  SLHHDKFVQFLRQLH+EIL LEFSHYDFKSQGSI+AKDFALS+V
Sbjct: 301 ENGGLVEYFFGQDGKGSLHHDKFVQFLRQLHDEILRLEFSHYDFKSQGSISAKDFALSMV 360

Query: 417 ASADINHIDKLLDRVEALNKEPYFRNIRITYQEFKDFAELRKKLESFSLAIFSYGKVNGA 476
           ASADINHIDKLLDRVEALNKEP FRNIRITY+EFKDFAELRKKLESFSLAIFSYGKVNG 
Sbjct: 361 ASADINHIDKLLDRVEALNKEPQFRNIRITYEEFKDFAELRKKLESFSLAIFSYGKVNGE 420

Query: 477 LTKKDFQRAASHVSTKNFNLKAQFNKLNQPNNHMHVAFLCKVCGVSITRNVVDIIFHIFD 536
           LTK+DFQRAASH                             VCGVSITRNVVDIIFHIFD
Sbjct: 421 LTKQDFQRAASH-----------------------------VCGVSITRNVVDIIFHIFD 480

Query: 537 VNADGDLSSDEFVRVVQRREVSSSQAAVGVAGILSCWFSCTAKCSSAKLFLRS 589
            N DGDLSSDEFVRV+QR+EVSSSQAAVG  G LSCWFSC+AKCSSAKLF+RS
Sbjct: 481 ANGDGDLSSDEFVRVIQRKEVSSSQAAVGAGGFLSCWFSCSAKCSSAKLFVRS 497

BLAST of Spg003495 vs. TAIR 10
Match: AT4G32060.1 (calcium-binding EF hand family protein )

HSP 1 Score: 463.8 bits (1192), Expect = 2.1e-130
Identity = 242/397 (60.96%), Postives = 299/397 (75.31%), Query Frame = 0

Query: 189 FLFGDDYRRKVFFNYEKRMRLQSPPEKVFEYFASIHTPEGEIFMTPADLMRAIVPVFPPS 248
           F+FGD YRRK+FFNYEKR+RLQSPPEKVFEYFAS+ T +GEI M PADLMRAIVPVFPPS
Sbjct: 132 FIFGDAYRRKIFFNYEKRLRLQSPPEKVFEYFASVRTDKGEILMKPADLMRAIVPVFPPS 191

Query: 249 ESNRVREGFLRGERRVHGELYCVPSKFFMLFDTNNDGLISFAEYIFFVTLLSIPESSFSV 308
           ES+ VREG+L GER   GEL C PS+FFMLFD +NDGLISF EYIFFVTLLSIPESSF+V
Sbjct: 192 ESHLVREGYLTGERN-PGELRCSPSEFFMLFDVDNDGLISFKEYIFFVTLLSIPESSFAV 251

Query: 309 AFKMFDIDNNGAIDREEFKKVMGLMRKQNRQGARHRDGRRLGMKV--CVENGGLVEYFFG 368
           AFKMFD DNNG ID+EEFK VM LMR Q+RQG  HRDG R G+ +   VE+GGLVEYFFG
Sbjct: 252 AFKMFDTDNNGEIDKEEFKTVMSLMRSQHRQGVGHRDGLRTGLHMTGSVEDGGLVEYFFG 311

Query: 369 QDGNDSLHHDKFVQFLRQLHEEILLLEFSHYDFKSQGSITAKDFALSVVASADINHIDKL 428
           +DG+  L HDKF +F++ L EE+L LEF+HYD+K +GSI+AKDFALS+VA+AD +H+ KL
Sbjct: 312 KDGSQKLKHDKFTKFMKDLTEEMLRLEFAHYDYKRRGSISAKDFALSMVAAADASHLSKL 371

Query: 429 LDRVEALNKEPYFRNIRITYQEFKDFAELRKKLESFSLAIFSYGKVNGALTKKDFQRAAS 488
           LDRVE+L++ P+ R++RI+ +EFK F ELR KL  FSLA+F+YGK NG LT KDF+RAAS
Sbjct: 372 LDRVESLSEHPHLRDMRISLKEFKQFDELRSKLGPFSLALFAYGKANGLLTMKDFKRAAS 431

Query: 489 HVSTKNFNLKAQFNKLNQPNNHMHVAFLCKVCGVSITRNVVDIIFHIFDVNADGDLSSDE 548
                                        +VCG++++ NV++I FH+FD N DG+LS DE
Sbjct: 432 -----------------------------QVCGITLSDNVIEIAFHVFDSNQDGNLSVDE 491

Query: 549 FVRVVQRREVSSSQ-AAVGVAGILSCWFSCTAKCSSA 583
           F+RV+ RRE   +Q  A G++   S  +  +  CSS+
Sbjct: 492 FLRVLHRRERDVAQPIAKGLSRYFSDGWKGSKNCSSS 498

BLAST of Spg003495 vs. TAIR 10
Match: AT4G32060.2 (calcium-binding EF hand family protein )

HSP 1 Score: 425.6 bits (1093), Expect = 6.3e-119
Identity = 212/300 (70.67%), Postives = 252/300 (84.00%), Query Frame = 0

Query: 189 FLFGDDYRRKVFFNYEKRMRLQSPPEKVFEYFASIHTPEGEIFMTPADLMRAIVPVFPPS 248
           F+FGD YRRK+FFNYEKR+RLQSPPEKVFEYFAS+ T +GEI M PADLMRAIVPVFPPS
Sbjct: 132 FIFGDAYRRKIFFNYEKRLRLQSPPEKVFEYFASVRTDKGEILMKPADLMRAIVPVFPPS 191

Query: 249 ESNRVREGFLRGERRVHGELYCVPSKFFMLFDTNNDGLISFAEYIFFVTLLSIPESSFSV 308
           ES+ VREG+L GER   GEL C PS+FFMLFD +NDGLISF EYIFFVTLLSIPESSF+V
Sbjct: 192 ESHLVREGYLTGERN-PGELRCSPSEFFMLFDVDNDGLISFKEYIFFVTLLSIPESSFAV 251

Query: 309 AFKMFDIDNNGAIDREEFKKVMGLMRKQNRQGARHRDGRRLGMKV--CVENGGLVEYFFG 368
           AFKMFD DNNG ID+EEFK VM LMR Q+RQG  HRDG R G+ +   VE+GGLVEYFFG
Sbjct: 252 AFKMFDTDNNGEIDKEEFKTVMSLMRSQHRQGVGHRDGLRTGLHMTGSVEDGGLVEYFFG 311

Query: 369 QDGNDSLHHDKFVQFLRQLHEEILLLEFSHYDFKSQGSITAKDFALSVVASADINHIDKL 428
           +DG+  L HDKF +F++ L EE+L LEF+HYD+K +GSI+AKDFALS+VA+AD +H+ KL
Sbjct: 312 KDGSQKLKHDKFTKFMKDLTEEMLRLEFAHYDYKRRGSISAKDFALSMVAAADASHLSKL 371

Query: 429 LDRVEALNKEPYFRNIRITYQEFKDFAELRKKLESFSLAIFSYGKVNGALTKKDFQRAAS 487
           LDRVE+L++ P+ R++RI+ +EFK F ELR KL  FSLA+F+YGK NG LT KDF+RAAS
Sbjct: 372 LDRVESLSEHPHLRDMRISLKEFKQFDELRSKLGPFSLALFAYGKANGLLTMKDFKRAAS 430

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008456564.11.7e-23882.68PREDICTED: calcium uptake protein 1, mitochondrial-like isoform X1 [Cucumis melo... [more]
KAA0063559.11.6e-23380.97calcium uptake protein 1 [Cucumis melo var. makuwa] >TYJ97885.1 calcium uptake p... [more]
XP_008456571.14.7e-23380.78PREDICTED: calcium uptake protein 1, mitochondrial-like isoform X2 [Cucumis melo... [more]
XP_004139397.16.6e-22779.30calcium uptake protein, mitochondrial [Cucumis sativus] >KGN60757.1 hypothetical... [more]
XP_022143166.11.1e-22679.17calcium uptake protein, mitochondrial-like [Momordica charantia][more]
Match NameE-valueIdentityDescription
Q9SZ453.0e-12960.96Calcium uptake protein, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=MICU PE... [more]
Q95PZ23.4e-4032.04Calcium uptake protein 1 homolog, mitochondrial OS=Caenorhabditis elegans OX=623... [more]
Q4R5183.4e-4030.49Calcium uptake protein 1, mitochondrial OS=Macaca fascicularis OX=9541 GN=MICU1 ... [more]
Q9BPX64.4e-4029.97Calcium uptake protein 1, mitochondrial OS=Homo sapiens OX=9606 GN=MICU1 PE=1 SV... [more]
B1H2N34.4e-4029.69Calcium uptake protein 1, mitochondrial OS=Xenopus tropicalis OX=8364 GN=micu1 P... [more]
Match NameE-valueIdentityDescription
A0A1S3C3I88.1e-23982.68calcium uptake protein 1, mitochondrial-like isoform X1 OS=Cucumis melo OX=3656 ... [more]
A0A5A7VDG37.8e-23480.97Calcium uptake protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol... [more]
A0A1S3C4U22.3e-23380.78calcium uptake protein 1, mitochondrial-like isoform X2 OS=Cucumis melo OX=3656 ... [more]
A0A0A0LJ543.2e-22779.30Calcium ion binding protein OS=Cucumis sativus OX=3659 GN=Csa_2G009450 PE=4 SV=1[more]
A0A6J1CPF95.4e-22779.17calcium uptake protein, mitochondrial-like OS=Momordica charantia OX=3673 GN=LOC... [more]
Match NameE-valueIdentityDescription
AT4G32060.12.1e-13060.96calcium-binding EF hand family protein [more]
AT4G32060.26.3e-11970.67calcium-binding EF hand family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainSMARTSM00054efh_1coord: 271..299
e-value: 0.032
score: 23.4
coord: 305..333
e-value: 6.8E-5
score: 32.3
coord: 526..554
e-value: 1.0
score: 17.7
IPR002048EF-hand domainPFAMPF13833EF-hand_8coord: 276..297
e-value: 0.0015
score: 18.3
IPR002048EF-hand domainPFAMPF13202EF-hand_5coord: 528..551
e-value: 0.0026
score: 17.2
IPR002048EF-hand domainPFAMPF00036EF-hand_1coord: 309..331
e-value: 1.8E-6
score: 27.1
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 301..336
score: 12.839682
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 522..557
score: 10.3012
IPR002048EF-hand domainCDDcd00051EFhcoord: 274..330
e-value: 6.00747E-11
score: 56.0169
NoneNo IPR availableGENE3D1.10.238.10coord: 210..350
e-value: 6.6E-15
score: 57.1
NoneNo IPR availableGENE3D1.10.238.10coord: 382..566
e-value: 8.0E-9
score: 37.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 85..119
NoneNo IPR availablePANTHERPTHR12294:SF23CALCIUM UPTAKE PROTEIN, MITOCHONDRIALcoord: 516..584
coord: 63..489
NoneNo IPR availableCDDcd15900EFh_MICUcoord: 306..554
e-value: 1.3263E-37
score: 134.278
IPR039800Calcium uptake protein 1/2/3PANTHERPTHR12294EF HAND DOMAIN FAMILY A1,A2-RELATEDcoord: 516..584
coord: 63..489
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 535..547
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 314..326
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 274..552

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg003495.1Spg003495.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0036444 calcium import into the mitochondrion
biological_process GO:0051560 mitochondrial calcium ion homeostasis
biological_process GO:0006851 mitochondrial calcium ion transmembrane transport
cellular_component GO:1990246 uniplex complex
molecular_function GO:0005509 calcium ion binding