Spg003402 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg003402 vs. NCBI nr
Match: XP_022138962.1 (1-aminocyclopropane-1-carboxylate oxidase 1 [Momordica charantia])

HSP 1 Score: 544.3 bits (1401), Expect = 6.9e-151
Identity = 258/297 (86.87%), Postives = 283/297 (95.29%), Query Frame = 0

Query: 13  MKIPTIDFNQLNGDNRAKTLSVLHDACKDWGFFLVENHGIEEEVLEKVKLLVNRHYDENM 72
           M+IPTIDFNQLNGDNRAKTLS+LH+AC++WGFF+VENHGIEEEVLEKVK LVNRHYDENM
Sbjct: 1   MEIPTIDFNQLNGDNRAKTLSLLHEACQNWGFFMVENHGIEEEVLEKVKELVNRHYDENM 60

Query: 73  KEKFYNSDVAKNLGKMAVSDVDWESTFFIWHKPNSNISQFTNVSQDLCKAMEEEYIPQVI 132
           KE FY+S+VAKNLGKM +SDVDWESTFFI H+P SNISQ TN+SQ LC+AMEE+YIPQ+I
Sbjct: 61  KESFYSSEVAKNLGKMPLSDVDWESTFFICHRPKSNISQITNISQHLCEAMEEDYIPQLI 120

Query: 133 KIAEKLAELMCENLGLEKAHIKQLFTGTKGPSVGTKVAKYPKCPRPDLVRGLREHTDAGG 192
           KIAE LAELMCENLGLEKAHIKQLF+GT GPSVGTKVAKYP+CPRP+LVRGLREHTDAGG
Sbjct: 121 KIAETLAELMCENLGLEKAHIKQLFSGTNGPSVGTKVAKYPECPRPELVRGLREHTDAGG 180

Query: 193 IILLLQDDQVPGLEFENDGVWFKIPPSKNNAIFVNIGDQIEVLSSGKYKSILHRVLAEKD 252
           IILLLQDDQVPGLEF+NDGVW KIPPSK NAIFVNIGDQIEV+S+G+YKSILHRVLAEK+
Sbjct: 181 IILLLQDDQVPGLEFQNDGVWLKIPPSKKNAIFVNIGDQIEVVSNGRYKSILHRVLAEKE 240

Query: 253 GSRLSIATFYNPATDAVISPAAELLYPGGYRFEDYLKFYNTTKFGDKEKRFQSMKRM 310
           GSR+SIATFYNPATDAVISPA ELLYPG YRFEDYLKFYNTTKFGDK +RFQSMK+M
Sbjct: 241 GSRISIATFYNPATDAVISPAPELLYPGEYRFEDYLKFYNTTKFGDKGRRFQSMKKM 297

BLAST of Spg003402 vs. NCBI nr
Match: XP_038890644.1 (1-aminocyclopropane-1-carboxylate oxidase 1 [Benincasa hispida])

HSP 1 Score: 537.3 bits (1383), Expect = 8.4e-149
Identity = 258/298 (86.58%), Postives = 279/298 (93.62%), Query Frame = 0

Query: 13  MKIPTIDFNQLNGDNRAKTLSVLHDACKDWGFFLVENHGIEEEVLEKVKLLVNRHYDENM 72
           MKIPTIDFNQL GDN+A  LSVLH+AC++WGFFLVENHGIEE VLEKVK LVN+HYD+NM
Sbjct: 3   MKIPTIDFNQLTGDNKANALSVLHEACQNWGFFLVENHGIEERVLEKVKFLVNKHYDDNM 62

Query: 73  KEKFYNSDVAKNLGKMAVSDVDWESTFFIWHKPNSNISQFTNVSQDLCKAMEEEYIPQVI 132
           KE+FYNSDVAKNLGKMA+++VDWESTFFI HKP SN+S FTN+SQ LCKAMEEEYIP+VI
Sbjct: 63  KEEFYNSDVAKNLGKMALTNVDWESTFFICHKPTSNLSHFTNISQHLCKAMEEEYIPEVI 122

Query: 133 KIAEKLAELMCENLGLEKAHIKQLFTGTKGPSVGTKVAKYPKCPRPDLVRGLREHTDAGG 192
           KIAEKLAELM ENLGL+KAHIKQLF GT GPSVGTKVAKYP+CPRP+LVRGLREHTDAGG
Sbjct: 123 KIAEKLAELMSENLGLQKAHIKQLFNGTNGPSVGTKVAKYPECPRPELVRGLREHTDAGG 182

Query: 193 IILLLQDDQVPGLEFENDGVWFKIPPSKNNAIFVNIGDQIEVLSSGKYKSILHRVLAEKD 252
           IILLLQDDQVPGLEF+ DG WFKIPPSKNNAIFVNIGDQIEVLS+GKYKSILHRVLAEKD
Sbjct: 183 IILLLQDDQVPGLEFQIDGGWFKIPPSKNNAIFVNIGDQIEVLSNGKYKSILHRVLAEKD 242

Query: 253 GSRLSIATFYNPATDAVISPAAELLYPGGYRFEDYLKFYNTTKFGDKEKRFQS-MKRM 310
           GSRLSIATFYNPATDA+ISPA ELLYPGGYRF DYLKFYNTTKFGDKE+RF   MK+M
Sbjct: 243 GSRLSIATFYNPATDALISPAPELLYPGGYRFGDYLKFYNTTKFGDKERRFSDFMKKM 300

BLAST of Spg003402 vs. NCBI nr
Match: XP_022965527.1 (1-aminocyclopropane-1-carboxylate oxidase 1 [Cucurbita maxima])

HSP 1 Score: 532.3 bits (1370), Expect = 2.7e-147
Identity = 252/297 (84.85%), Postives = 277/297 (93.27%), Query Frame = 0

Query: 13  MKIPTIDFNQLNGDNRAKTLSVLHDACKDWGFFLVENHGIEEEVLEKVKLLVNRHYDENM 72
           MKIPTIDF+++NGDNRA+ LSVLHDAC +WGFF++ENHGIEE VLEKVKLLVN HYDE M
Sbjct: 1   MKIPTIDFSEVNGDNRARALSVLHDACLNWGFFMIENHGIEEAVLEKVKLLVNTHYDETM 60

Query: 73  KEKFYNSDVAKNLGKMAVSDVDWESTFFIWHKPNSNISQFTNVSQDLCKAMEEEYIPQVI 132
           K+KFY+S+VAKNLGK+ + DVDWESTFFI HKP SNIS+F N+S  LCKAMEEEYIPQ++
Sbjct: 61  KDKFYDSEVAKNLGKIQLFDVDWESTFFISHKPTSNISRFPNISDHLCKAMEEEYIPQLV 120

Query: 133 KIAEKLAELMCENLGLEKAHIKQLFTGTKGPSVGTKVAKYPKCPRPDLVRGLREHTDAGG 192
            IAEKLAELMCENLGL+K +IKQLF GTKGPSVGTKVAKYP+CPRP+LVRGLREHTDAGG
Sbjct: 121 MIAEKLAELMCENLGLQKTYIKQLFNGTKGPSVGTKVAKYPECPRPELVRGLREHTDAGG 180

Query: 193 IILLLQDDQVPGLEFENDGVWFKIPPSKNNAIFVNIGDQIEVLSSGKYKSILHRVLAEKD 252
           IILLLQDD+VPGLEF+ DG WFKIPPSKNNAIFVNIGDQIEVL++GKYKSILHRVLAEK+
Sbjct: 181 IILLLQDDKVPGLEFQKDGEWFKIPPSKNNAIFVNIGDQIEVLTNGKYKSILHRVLAEKE 240

Query: 253 GSRLSIATFYNPATDAVISPAAELLYPGGYRFEDYLKFYNTTKFGDKEKRFQSMKRM 310
           GSRLSIATFYNPATDAVISPAAE LYPGGYRFEDYLKFYNTTKFGDKEKRF SMK M
Sbjct: 241 GSRLSIATFYNPATDAVISPAAEALYPGGYRFEDYLKFYNTTKFGDKEKRFGSMKNM 297

BLAST of Spg003402 vs. NCBI nr
Match: TYK10774.1 (1-aminocyclopropane-1-carboxylate oxidase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 530.0 bits (1364), Expect = 1.3e-146
Identity = 253/300 (84.33%), Postives = 278/300 (92.67%), Query Frame = 0

Query: 13  MKIPTIDFNQLNGDNRAKTLSVLHDACKDWGFFLVENHGIEEEVLEKVKLLVNRHYDENM 72
           MKIPTIDFNQLN DN+  TLS+LH+AC++WGFFLVENHGIEE VLEKVKLLVN+HYDENM
Sbjct: 1   MKIPTIDFNQLNEDNKGNTLSILHEACQNWGFFLVENHGIEERVLEKVKLLVNKHYDENM 60

Query: 73  KEKFYNSDVAKNLGKMAVSDVDWESTFFIWHKPNSNISQFTNVSQDLCKAMEEEYIPQVI 132
           KE+FYNSDVAKNLGK+ +S+VDWESTFFI HKP SN+SQF N+SQ LCKAMEEEYIP++I
Sbjct: 61  KEEFYNSDVAKNLGKITLSNVDWESTFFISHKPTSNVSQFCNISQPLCKAMEEEYIPEII 120

Query: 133 KIAEKLAELMCENLGLEKAHIKQLFTGTKGPSVGTKVAKYPKCPRPDLVRGLREHTDAGG 192
           KIAEKL+ELMCENLGL+K+HIKQLF GT GPSVGTKVAKYP+CP+PDLVRGLREHTDAGG
Sbjct: 121 KIAEKLSELMCENLGLQKSHIKQLFNGTNGPSVGTKVAKYPECPQPDLVRGLREHTDAGG 180

Query: 193 IILLLQDDQVPGLEFENDGVWFKIPPSKNNAIFVNIGDQIEVLSSGKYKSILHRVLAEKD 252
           IILLLQDDQVPGLEF+ DG WFKIPPSKNNAIFVNIGDQIEVL++GKYKSILHRVLAEK 
Sbjct: 181 IILLLQDDQVPGLEFQIDGRWFKIPPSKNNAIFVNIGDQIEVLTNGKYKSILHRVLAEKH 240

Query: 253 GSRLSIATFYNPATDAVISPAAELLYPGGYRFEDYLKFYNTTKFGDKEKR---FQSMKRM 310
           GSRLSIATFYNPATDA+I PA EL YPGGYRF DYLKFYNTTKFGDKEKR   FQS+ +M
Sbjct: 241 GSRLSIATFYNPATDALIFPAPELSYPGGYRFGDYLKFYNTTKFGDKEKRFSDFQSINKM 300

BLAST of Spg003402 vs. NCBI nr
Match: XP_008459274.1 (PREDICTED: 1-aminocyclopropane-1-carboxylate oxidase 1 [Cucumis melo])

HSP 1 Score: 528.9 bits (1361), Expect = 3.0e-146
Identity = 252/300 (84.00%), Postives = 278/300 (92.67%), Query Frame = 0

Query: 13  MKIPTIDFNQLNGDNRAKTLSVLHDACKDWGFFLVENHGIEEEVLEKVKLLVNRHYDENM 72
           MKIPTIDFNQLN DN+  TLS+LH+AC++WGFFLVENHGIEE VLEKVKLLVN+HYDENM
Sbjct: 1   MKIPTIDFNQLNEDNKGNTLSILHEACQNWGFFLVENHGIEERVLEKVKLLVNKHYDENM 60

Query: 73  KEKFYNSDVAKNLGKMAVSDVDWESTFFIWHKPNSNISQFTNVSQDLCKAMEEEYIPQVI 132
           KE+FYNSDVAKNLGK+ +S++DWESTFFI HKP SN+SQF N+SQ LCKAMEEEYIP++I
Sbjct: 61  KEEFYNSDVAKNLGKITLSNMDWESTFFISHKPTSNVSQFCNISQPLCKAMEEEYIPEII 120

Query: 133 KIAEKLAELMCENLGLEKAHIKQLFTGTKGPSVGTKVAKYPKCPRPDLVRGLREHTDAGG 192
           KIAEKL+ELMCENLGL+K+HIKQLF GT GPSVGTKVAKYP+CP+PDLVRGLREHTDAGG
Sbjct: 121 KIAEKLSELMCENLGLQKSHIKQLFNGTNGPSVGTKVAKYPECPQPDLVRGLREHTDAGG 180

Query: 193 IILLLQDDQVPGLEFENDGVWFKIPPSKNNAIFVNIGDQIEVLSSGKYKSILHRVLAEKD 252
           IILLLQDDQVPGLEF+ DG WFKIPPSKNNAIFVNIGDQIEVL++GKYKSILHRVLAEK 
Sbjct: 181 IILLLQDDQVPGLEFQIDGRWFKIPPSKNNAIFVNIGDQIEVLTNGKYKSILHRVLAEKH 240

Query: 253 GSRLSIATFYNPATDAVISPAAELLYPGGYRFEDYLKFYNTTKFGDKEKR---FQSMKRM 310
           GSRLSIATFYNPATDA+I PA EL YPGGYRF DYLKFYNTTKFGDKEKR   FQS+ +M
Sbjct: 241 GSRLSIATFYNPATDALIFPAPELSYPGGYRFGDYLKFYNTTKFGDKEKRFSDFQSINKM 300

BLAST of Spg003402 vs. ExPASy Swiss-Prot
Match: Q9ZUN4 (1-aminocyclopropane-1-carboxylate oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=ACO1 PE=2 SV=1)

HSP 1 Score: 422.2 bits (1084), Expect = 5.2e-117
Identity = 194/301 (64.45%), Postives = 247/301 (82.06%), Query Frame = 0

Query: 9   KGKNMKIPTIDFNQLNGDNRAKTLSVLHDACKDWGFFLVENHGIEEEVLEKVKLLVNRHY 68
           K + M+IP IDF +L+G+ R+KT+S+L  AC  WGFF+V+NHGI++E++EKVK ++N HY
Sbjct: 5   KEREMEIPVIDFAELDGEKRSKTMSLLDHACDKWGFFMVDNHGIDKELMEKVKKMINSHY 64

Query: 69  DENMKEKFYNSDVAKNLGKMAVSDVDWESTFFIWHKPNSNISQFTNVSQDLCKAMEEEYI 128
           +E++KEKFY S++ K L +   SD DWES+FFI HKP SNI Q  N+S++L K M +EY+
Sbjct: 65  EEHLKEKFYQSEMVKALSEGKTSDADWESSFFISHKPTSNICQIPNISEELSKTM-DEYV 124

Query: 129 PQVIKIAEKLAELMCENLGLEKAHIKQLFTGTKGPSVGTKVAKYPKCPRPDLVRGLREHT 188
            Q+ K AE+L++LMCENLGL++  I   F+G KGP+ GTKVAKYP+CPRP+L+RGLREHT
Sbjct: 125 CQLHKFAERLSKLMCENLGLDQEDIMNAFSGPKGPAFGTKVAKYPECPRPELMRGLREHT 184

Query: 189 DAGGIILLLQDDQVPGLEFENDGVWFKIPPSKNNAIFVNIGDQIEVLSSGKYKSILHRVL 248
           DAGGIILLLQDDQVPGLEF  DG W  IPPSKNN IFVN GDQ+E+LS+G+YKS++HRV+
Sbjct: 185 DAGGIILLLQDDQVPGLEFFKDGKWVPIPPSKNNTIFVNTGDQLEILSNGRYKSVVHRVM 244

Query: 249 AEKDGSRLSIATFYNPATDAVISPAAELLYPGGYRFEDYLKFYNTTKFGDKEKRFQSMKR 308
             K GSRLSIATFYNPA DA+ISPA +LLYP GYRF+DYLK Y+TTKFGDK  R ++MK+
Sbjct: 245 TVKHGSRLSIATFYNPAGDAIISPAPKLLYPSGYRFQDYLKLYSTTKFGDKGPRLETMKK 304

Query: 309 M 310
           M
Sbjct: 305 M 304

BLAST of Spg003402 vs. ExPASy Swiss-Prot
Match: O48882 (1-aminocyclopropane-1-carboxylate oxidase 2 OS=Malus domestica OX=3750 GN=ACO2 PE=2 SV=1)

HSP 1 Score: 315.8 bits (808), Expect = 5.2e-85
Identity = 152/303 (50.17%), Postives = 217/303 (71.62%), Query Frame = 0

Query: 16  PTIDFNQLNGDNRAKTLSVLHDACKDWGFFLVENHGIEEEVLEKVKLLVNRHYDENMKEK 75
           P +D + +NG+ RA TL  ++DAC++WGFF + NHGI  E+L+ V+ +   HY + M+++
Sbjct: 5   PVVDMDLINGEERAATLEKINDACENWGFFELVNHGISTELLDTVEKMNKDHYKKTMEQR 64

Query: 76  FYNSDVAKNLG--KMAVSDVDWESTFFIWHKPNSNISQFTNVSQDLCKAMEEEYIPQVIK 135
           F     AK L   +  +  +DWESTFF+ H P+SNIS+  ++ +D  K M +E+  ++ K
Sbjct: 65  FKEMVAAKGLEAVQSEIHYLDWESTFFLRHLPSSNISEIPDLEEDYRKTM-KEFAVELEK 124

Query: 136 IAEKLAELMCENLGLEKAHIKQLFTGTKGPSVGTKVAKYPKCPRPDLVRGLREHTDAGGI 195
           +AEKL +L+CENLGLEK ++K+ F G+KGP+ GTKV+ YP CP+PDL++GLR HTDAGGI
Sbjct: 125 LAEKLLDLLCENLGLEKGYLKKAFYGSKGPNFGTKVSNYPPCPKPDLIKGLRAHTDAGGI 184

Query: 196 ILLLQDDQVPGLEFENDGVWFKIPPSKNNAIFVNIGDQIEVLSSGKYKSILHRVLAEKDG 255
           ILL QDD+V GL+   DG W  +PP  +++I +N+GDQIEV+++GKYKSI+HRV+A+ DG
Sbjct: 185 ILLFQDDKVSGLQLLKDGEWMDVPP-VHHSIVINLGDQIEVITNGKYKSIMHRVIAQSDG 244

Query: 256 SRLSIATFYNPATDAVISPAAELL---------YPGGYRFEDYLKFYNTTKFGDKEKRFQ 308
           +R+SIA+FYNP  DA ISPA  LL         YP  + F+DY+K Y+  KF  KE RF+
Sbjct: 245 TRMSIASFYNPGDDAFISPAPALLEEKSEVSPTYP-KFLFDDYMKLYSGLKFQAKEPRFE 304

BLAST of Spg003402 vs. ExPASy Swiss-Prot
Match: P31237 (1-aminocyclopropane-1-carboxylate oxidase OS=Actinidia deliciosa OX=3627 GN=ACO PE=2 SV=1)

HSP 1 Score: 315.8 bits (808), Expect = 5.2e-85
Identity = 152/306 (49.67%), Postives = 219/306 (71.57%), Query Frame = 0

Query: 16  PTIDFNQLNGDNRAKTLSVLHDACKDWGFFLVENHGIEEEVLEKVKLLVNRHYDENMKEK 75
           P ID  +LNG+ RA T+  + DAC++WGFF + NHGI  E+++ V+ L   HY++ M+++
Sbjct: 5   PVIDMEKLNGEERAPTMEKIKDACENWGFFELVNHGISHELMDTVERLTKEHYNKCMEQR 64

Query: 76  FYNSDVAKNLG--KMAVSDVDWESTFFIWHKPNSNISQFTNVSQDLCKAMEEEYIPQVIK 135
           F      K L   +  ++D+DWESTFF+ H P SNIS+  ++ QD  KAM +E+  ++ K
Sbjct: 65  FKEMVATKGLEAVQSEINDLDWESTFFLRHLPVSNISEIPDLEQDHRKAM-KEFAEKLEK 124

Query: 136 IAEKLAELMCENLGLEKAHIKQLFTGTKGPSVGTKVAKYPKCPRPDLVRGLREHTDAGGI 195
           +AE+L +L+CEN+GLEK ++K+ F G+KGP+ GTKV+ YP CPRP+L++GLR HTDAGGI
Sbjct: 125 LAEQLLDLLCENVGLEKGYLKKAFYGSKGPTFGTKVSNYPPCPRPELIKGLRAHTDAGGI 184

Query: 196 ILLLQDDQVPGLEFENDGVWFKIPPSKNNAIFVNIGDQIEVLSSGKYKSILHRVLAEKDG 255
           ILL QD++V GL+   DG W  +PP K ++I +NIGDQ+EV+++GKYKS++HRV+A+ DG
Sbjct: 185 ILLFQDNKVSGLQLLKDGEWIDVPPMK-HSIVINIGDQLEVITNGKYKSVMHRVIAQPDG 244

Query: 256 SRLSIATFYNPATDAVISPAAEL----------LYPGGYRFEDYLKFYNTTKFGDKEKRF 310
           +R+SIA+FYNP +DAV+ PA  L          +YP  + FEDY+K Y   KF  KE RF
Sbjct: 245 NRMSIASFYNPGSDAVMYPAPALVDKEEDQQKQVYP-KFVFEDYMKLYAGLKFQAKEPRF 304

BLAST of Spg003402 vs. ExPASy Swiss-Prot
Match: Q00985 (1-aminocyclopropane-1-carboxylate oxidase 1 OS=Malus domestica OX=3750 PE=1 SV=1)

HSP 1 Score: 314.3 bits (804), Expect = 1.5e-84
Identity = 147/303 (48.51%), Postives = 219/303 (72.28%), Query Frame = 0

Query: 16  PTIDFNQLNGDNRAKTLSVLHDACKDWGFFLVENHGIEEEVLEKVKLLVNRHYDENMKEK 75
           P +D + +NG+ RA TL  ++DAC++WGFF + NHG+  E+L+ V+ +   HY + M+++
Sbjct: 5   PVVDLSLVNGEERAATLEKINDACENWGFFELVNHGMSTELLDTVEKMTKDHYKKTMEQR 64

Query: 76  FYNSDVAKNLG--KMAVSDVDWESTFFIWHKPNSNISQFTNVSQDLCKAMEEEYIPQVIK 135
           F     AK L   +  + D+DWESTFF+ H P+SNIS+  ++ ++  K M +E+  ++ K
Sbjct: 65  FKEMVAAKGLDDVQSEIHDLDWESTFFLRHLPSSNISEIPDLEEEYRKTM-KEFAVELEK 124

Query: 136 IAEKLAELMCENLGLEKAHIKQLFTGTKGPSVGTKVAKYPKCPRPDLVRGLREHTDAGGI 195
           +AEKL +L+CENLGLEK ++K++F G+KGP+ GTKV+ YP CP+PDL++GLR H+DAGGI
Sbjct: 125 LAEKLLDLLCENLGLEKGYLKKVFYGSKGPNFGTKVSNYPPCPKPDLIKGLRAHSDAGGI 184

Query: 196 ILLLQDDQVPGLEFENDGVWFKIPPSKNNAIFVNIGDQIEVLSSGKYKSILHRVLAEKDG 255
           ILL QDD+V GL+   DG W  +PP  +++I +N+GDQIEV+++GKYKS++HRV+A+ DG
Sbjct: 185 ILLFQDDKVSGLQLLKDGEWVDVPP-MHHSIVINLGDQIEVITNGKYKSVMHRVIAQSDG 244

Query: 256 SRLSIATFYNPATDAVISPAAELL---------YPGGYRFEDYLKFYNTTKFGDKEKRFQ 308
           +R+SIA+FYNP  D+ ISPA  +L         YP  + F+DY+K Y+  KF  KE RF+
Sbjct: 245 TRMSIASFYNPGNDSFISPAPAVLEKKTEDAPTYP-KFVFDDYMKLYSGLKFQAKEPRFE 304

BLAST of Spg003402 vs. ExPASy Swiss-Prot
Match: Q41931 (1-aminocyclopropane-1-carboxylate oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=ACO2 PE=1 SV=2)

HSP 1 Score: 313.9 bits (803), Expect = 2.0e-84
Identity = 143/304 (47.04%), Postives = 222/304 (73.03%), Query Frame = 0

Query: 11  KNMKIPTIDFNQLNGDNRAKTLSVLHDACKDWGFFLVENHGIEEEVLEKVKLLVNRHYDE 70
           KNMK P +D ++LNG+ R +T++++++AC++WGFF + NHG+  ++++K++ +   HY  
Sbjct: 3   KNMKFPVVDLSKLNGEERDQTMALINEACENWGFFEIVNHGLPHDLMDKIEKMTKDHYKT 62

Query: 71  NMKEKFYNSDVAKNLGKM--AVSDVDWESTFFIWHKPNSNISQFTNVSQDLCKAMEEEYI 130
             ++KF +   +K L  +   V DVDWESTF++ H P SN++  ++VS +   AM +++ 
Sbjct: 63  CQEQKFNDMLKSKGLDNLETEVEDVDWESTFYVRHLPQSNLNDISDVSDEYRTAM-KDFG 122

Query: 131 PQVIKIAEKLAELMCENLGLEKAHIKQLFTGTKGPSVGTKVAKYPKCPRPDLVRGLREHT 190
            ++  +AE L +L+CENLGLEK ++K++F GTKGP+ GTKV+ YP CP+P++++GLR HT
Sbjct: 123 KRLENLAEDLLDLLCENLGLEKGYLKKVFHGTKGPTFGTKVSNYPPCPKPEMIKGLRAHT 182

Query: 191 DAGGIILLLQDDQVPGLEFENDGVWFKIPPSKNNAIFVNIGDQIEVLSSGKYKSILHRVL 250
           DAGGIILL QDD+V GL+   DG W  +PP  N++I +N+GDQ+EV+++GKYKS+LHRV+
Sbjct: 183 DAGGIILLFQDDKVSGLQLLKDGDWIDVPP-LNHSIVINLGDQLEVITNGKYKSVLHRVV 242

Query: 251 AEKDGSRLSIATFYNPATDAVISPAAELL-----YPGGYRFEDYLKFYNTTKFGDKEKRF 308
            +++G+R+S+A+FYNP +DA ISPA  L+     YP  + F+DY+K Y   KF  KE RF
Sbjct: 243 TQQEGNRMSVASFYNPGSDAEISPATSLVEKDSEYP-SFVFDDYMKLYAGVKFQPKEPRF 302

BLAST of Spg003402 vs. ExPASy TrEMBL
Match: A0A6J1CCP2 (1-aminocyclopropane-1-carboxylate oxidase 1 OS=Momordica charantia OX=3673 GN=LOC111010009 PE=3 SV=1)

HSP 1 Score: 544.3 bits (1401), Expect = 3.3e-151
Identity = 258/297 (86.87%), Postives = 283/297 (95.29%), Query Frame = 0

Query: 13  MKIPTIDFNQLNGDNRAKTLSVLHDACKDWGFFLVENHGIEEEVLEKVKLLVNRHYDENM 72
           M+IPTIDFNQLNGDNRAKTLS+LH+AC++WGFF+VENHGIEEEVLEKVK LVNRHYDENM
Sbjct: 1   MEIPTIDFNQLNGDNRAKTLSLLHEACQNWGFFMVENHGIEEEVLEKVKELVNRHYDENM 60

Query: 73  KEKFYNSDVAKNLGKMAVSDVDWESTFFIWHKPNSNISQFTNVSQDLCKAMEEEYIPQVI 132
           KE FY+S+VAKNLGKM +SDVDWESTFFI H+P SNISQ TN+SQ LC+AMEE+YIPQ+I
Sbjct: 61  KESFYSSEVAKNLGKMPLSDVDWESTFFICHRPKSNISQITNISQHLCEAMEEDYIPQLI 120

Query: 133 KIAEKLAELMCENLGLEKAHIKQLFTGTKGPSVGTKVAKYPKCPRPDLVRGLREHTDAGG 192
           KIAE LAELMCENLGLEKAHIKQLF+GT GPSVGTKVAKYP+CPRP+LVRGLREHTDAGG
Sbjct: 121 KIAETLAELMCENLGLEKAHIKQLFSGTNGPSVGTKVAKYPECPRPELVRGLREHTDAGG 180

Query: 193 IILLLQDDQVPGLEFENDGVWFKIPPSKNNAIFVNIGDQIEVLSSGKYKSILHRVLAEKD 252
           IILLLQDDQVPGLEF+NDGVW KIPPSK NAIFVNIGDQIEV+S+G+YKSILHRVLAEK+
Sbjct: 181 IILLLQDDQVPGLEFQNDGVWLKIPPSKKNAIFVNIGDQIEVVSNGRYKSILHRVLAEKE 240

Query: 253 GSRLSIATFYNPATDAVISPAAELLYPGGYRFEDYLKFYNTTKFGDKEKRFQSMKRM 310
           GSR+SIATFYNPATDAVISPA ELLYPG YRFEDYLKFYNTTKFGDK +RFQSMK+M
Sbjct: 241 GSRISIATFYNPATDAVISPAPELLYPGEYRFEDYLKFYNTTKFGDKGRRFQSMKKM 297

BLAST of Spg003402 vs. ExPASy TrEMBL
Match: A0A6J1HKJ9 (1-aminocyclopropane-1-carboxylate oxidase 1 OS=Cucurbita maxima OX=3661 GN=LOC111465402 PE=3 SV=1)

HSP 1 Score: 532.3 bits (1370), Expect = 1.3e-147
Identity = 252/297 (84.85%), Postives = 277/297 (93.27%), Query Frame = 0

Query: 13  MKIPTIDFNQLNGDNRAKTLSVLHDACKDWGFFLVENHGIEEEVLEKVKLLVNRHYDENM 72
           MKIPTIDF+++NGDNRA+ LSVLHDAC +WGFF++ENHGIEE VLEKVKLLVN HYDE M
Sbjct: 1   MKIPTIDFSEVNGDNRARALSVLHDACLNWGFFMIENHGIEEAVLEKVKLLVNTHYDETM 60

Query: 73  KEKFYNSDVAKNLGKMAVSDVDWESTFFIWHKPNSNISQFTNVSQDLCKAMEEEYIPQVI 132
           K+KFY+S+VAKNLGK+ + DVDWESTFFI HKP SNIS+F N+S  LCKAMEEEYIPQ++
Sbjct: 61  KDKFYDSEVAKNLGKIQLFDVDWESTFFISHKPTSNISRFPNISDHLCKAMEEEYIPQLV 120

Query: 133 KIAEKLAELMCENLGLEKAHIKQLFTGTKGPSVGTKVAKYPKCPRPDLVRGLREHTDAGG 192
            IAEKLAELMCENLGL+K +IKQLF GTKGPSVGTKVAKYP+CPRP+LVRGLREHTDAGG
Sbjct: 121 MIAEKLAELMCENLGLQKTYIKQLFNGTKGPSVGTKVAKYPECPRPELVRGLREHTDAGG 180

Query: 193 IILLLQDDQVPGLEFENDGVWFKIPPSKNNAIFVNIGDQIEVLSSGKYKSILHRVLAEKD 252
           IILLLQDD+VPGLEF+ DG WFKIPPSKNNAIFVNIGDQIEVL++GKYKSILHRVLAEK+
Sbjct: 181 IILLLQDDKVPGLEFQKDGEWFKIPPSKNNAIFVNIGDQIEVLTNGKYKSILHRVLAEKE 240

Query: 253 GSRLSIATFYNPATDAVISPAAELLYPGGYRFEDYLKFYNTTKFGDKEKRFQSMKRM 310
           GSRLSIATFYNPATDAVISPAAE LYPGGYRFEDYLKFYNTTKFGDKEKRF SMK M
Sbjct: 241 GSRLSIATFYNPATDAVISPAAEALYPGGYRFEDYLKFYNTTKFGDKEKRFGSMKNM 297

BLAST of Spg003402 vs. ExPASy TrEMBL
Match: A0A5D3CHV3 (1-aminocyclopropane-1-carboxylate oxidase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold332G00870 PE=3 SV=1)

HSP 1 Score: 530.0 bits (1364), Expect = 6.5e-147
Identity = 253/300 (84.33%), Postives = 278/300 (92.67%), Query Frame = 0

Query: 13  MKIPTIDFNQLNGDNRAKTLSVLHDACKDWGFFLVENHGIEEEVLEKVKLLVNRHYDENM 72
           MKIPTIDFNQLN DN+  TLS+LH+AC++WGFFLVENHGIEE VLEKVKLLVN+HYDENM
Sbjct: 1   MKIPTIDFNQLNEDNKGNTLSILHEACQNWGFFLVENHGIEERVLEKVKLLVNKHYDENM 60

Query: 73  KEKFYNSDVAKNLGKMAVSDVDWESTFFIWHKPNSNISQFTNVSQDLCKAMEEEYIPQVI 132
           KE+FYNSDVAKNLGK+ +S+VDWESTFFI HKP SN+SQF N+SQ LCKAMEEEYIP++I
Sbjct: 61  KEEFYNSDVAKNLGKITLSNVDWESTFFISHKPTSNVSQFCNISQPLCKAMEEEYIPEII 120

Query: 133 KIAEKLAELMCENLGLEKAHIKQLFTGTKGPSVGTKVAKYPKCPRPDLVRGLREHTDAGG 192
           KIAEKL+ELMCENLGL+K+HIKQLF GT GPSVGTKVAKYP+CP+PDLVRGLREHTDAGG
Sbjct: 121 KIAEKLSELMCENLGLQKSHIKQLFNGTNGPSVGTKVAKYPECPQPDLVRGLREHTDAGG 180

Query: 193 IILLLQDDQVPGLEFENDGVWFKIPPSKNNAIFVNIGDQIEVLSSGKYKSILHRVLAEKD 252
           IILLLQDDQVPGLEF+ DG WFKIPPSKNNAIFVNIGDQIEVL++GKYKSILHRVLAEK 
Sbjct: 181 IILLLQDDQVPGLEFQIDGRWFKIPPSKNNAIFVNIGDQIEVLTNGKYKSILHRVLAEKH 240

Query: 253 GSRLSIATFYNPATDAVISPAAELLYPGGYRFEDYLKFYNTTKFGDKEKR---FQSMKRM 310
           GSRLSIATFYNPATDA+I PA EL YPGGYRF DYLKFYNTTKFGDKEKR   FQS+ +M
Sbjct: 241 GSRLSIATFYNPATDALIFPAPELSYPGGYRFGDYLKFYNTTKFGDKEKRFSDFQSINKM 300

BLAST of Spg003402 vs. ExPASy TrEMBL
Match: A0A1S3CB09 (1-aminocyclopropane-1-carboxylate oxidase 1 OS=Cucumis melo OX=3656 GN=LOC103498449 PE=3 SV=1)

HSP 1 Score: 528.9 bits (1361), Expect = 1.5e-146
Identity = 252/300 (84.00%), Postives = 278/300 (92.67%), Query Frame = 0

Query: 13  MKIPTIDFNQLNGDNRAKTLSVLHDACKDWGFFLVENHGIEEEVLEKVKLLVNRHYDENM 72
           MKIPTIDFNQLN DN+  TLS+LH+AC++WGFFLVENHGIEE VLEKVKLLVN+HYDENM
Sbjct: 1   MKIPTIDFNQLNEDNKGNTLSILHEACQNWGFFLVENHGIEERVLEKVKLLVNKHYDENM 60

Query: 73  KEKFYNSDVAKNLGKMAVSDVDWESTFFIWHKPNSNISQFTNVSQDLCKAMEEEYIPQVI 132
           KE+FYNSDVAKNLGK+ +S++DWESTFFI HKP SN+SQF N+SQ LCKAMEEEYIP++I
Sbjct: 61  KEEFYNSDVAKNLGKITLSNMDWESTFFISHKPTSNVSQFCNISQPLCKAMEEEYIPEII 120

Query: 133 KIAEKLAELMCENLGLEKAHIKQLFTGTKGPSVGTKVAKYPKCPRPDLVRGLREHTDAGG 192
           KIAEKL+ELMCENLGL+K+HIKQLF GT GPSVGTKVAKYP+CP+PDLVRGLREHTDAGG
Sbjct: 121 KIAEKLSELMCENLGLQKSHIKQLFNGTNGPSVGTKVAKYPECPQPDLVRGLREHTDAGG 180

Query: 193 IILLLQDDQVPGLEFENDGVWFKIPPSKNNAIFVNIGDQIEVLSSGKYKSILHRVLAEKD 252
           IILLLQDDQVPGLEF+ DG WFKIPPSKNNAIFVNIGDQIEVL++GKYKSILHRVLAEK 
Sbjct: 181 IILLLQDDQVPGLEFQIDGRWFKIPPSKNNAIFVNIGDQIEVLTNGKYKSILHRVLAEKH 240

Query: 253 GSRLSIATFYNPATDAVISPAAELLYPGGYRFEDYLKFYNTTKFGDKEKR---FQSMKRM 310
           GSRLSIATFYNPATDA+I PA EL YPGGYRF DYLKFYNTTKFGDKEKR   FQS+ +M
Sbjct: 241 GSRLSIATFYNPATDALIFPAPELSYPGGYRFGDYLKFYNTTKFGDKEKRFSDFQSINKM 300

BLAST of Spg003402 vs. ExPASy TrEMBL
Match: A0A5A7V755 (ACC oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold41G00590 PE=3 SV=1)

HSP 1 Score: 527.3 bits (1357), Expect = 4.2e-146
Identity = 252/300 (84.00%), Postives = 276/300 (92.00%), Query Frame = 0

Query: 13  MKIPTIDFNQLNGDNRAKTLSVLHDACKDWGFFLVENHGIEEEVLEKVKLLVNRHYDENM 72
           MKIPTIDFNQLN DN   TLS+LH+AC++WGFFLVENHGIEE VLEKVKLLVN+HYDENM
Sbjct: 1   MKIPTIDFNQLNEDNEGNTLSILHEACQNWGFFLVENHGIEERVLEKVKLLVNKHYDENM 60

Query: 73  KEKFYNSDVAKNLGKMAVSDVDWESTFFIWHKPNSNISQFTNVSQDLCKAMEEEYIPQVI 132
           KE+FYNSDVAKNLGK+ +S+VDWESTFFI HKP SN+SQF N+ Q LCKAMEEEYIP++I
Sbjct: 61  KEEFYNSDVAKNLGKITLSNVDWESTFFISHKPTSNVSQFCNIPQPLCKAMEEEYIPEII 120

Query: 133 KIAEKLAELMCENLGLEKAHIKQLFTGTKGPSVGTKVAKYPKCPRPDLVRGLREHTDAGG 192
           KIAEKL+ELMCENLGL+K+HIKQLF GT GPSVGTKVAKYP+CP+PDLVRGLREHTDAGG
Sbjct: 121 KIAEKLSELMCENLGLQKSHIKQLFNGTNGPSVGTKVAKYPECPQPDLVRGLREHTDAGG 180

Query: 193 IILLLQDDQVPGLEFENDGVWFKIPPSKNNAIFVNIGDQIEVLSSGKYKSILHRVLAEKD 252
           IILLLQDDQVPGLEF+ DG WFKIPPSKNNAIFVNIGDQIEVL++GKYKSILHRVLAEK 
Sbjct: 181 IILLLQDDQVPGLEFQIDGRWFKIPPSKNNAIFVNIGDQIEVLTNGKYKSILHRVLAEKH 240

Query: 253 GSRLSIATFYNPATDAVISPAAELLYPGGYRFEDYLKFYNTTKFGDKEKR---FQSMKRM 310
           GSRLSIATFYNPATDA+I PA EL YPGGYRF DYLKFYNTTKFGDKEKR   FQS+ +M
Sbjct: 241 GSRLSIATFYNPATDALIFPAPELSYPGGYRFGDYLKFYNTTKFGDKEKRFSDFQSINKM 300

BLAST of Spg003402 vs. TAIR 10
Match: AT2G19590.1 (ACC oxidase 1 )

HSP 1 Score: 422.2 bits (1084), Expect = 3.7e-118
Identity = 194/301 (64.45%), Postives = 247/301 (82.06%), Query Frame = 0

Query: 9   KGKNMKIPTIDFNQLNGDNRAKTLSVLHDACKDWGFFLVENHGIEEEVLEKVKLLVNRHY 68
           K + M+IP IDF +L+G+ R+KT+S+L  AC  WGFF+V+NHGI++E++EKVK ++N HY
Sbjct: 5   KEREMEIPVIDFAELDGEKRSKTMSLLDHACDKWGFFMVDNHGIDKELMEKVKKMINSHY 64

Query: 69  DENMKEKFYNSDVAKNLGKMAVSDVDWESTFFIWHKPNSNISQFTNVSQDLCKAMEEEYI 128
           +E++KEKFY S++ K L +   SD DWES+FFI HKP SNI Q  N+S++L K M +EY+
Sbjct: 65  EEHLKEKFYQSEMVKALSEGKTSDADWESSFFISHKPTSNICQIPNISEELSKTM-DEYV 124

Query: 129 PQVIKIAEKLAELMCENLGLEKAHIKQLFTGTKGPSVGTKVAKYPKCPRPDLVRGLREHT 188
            Q+ K AE+L++LMCENLGL++  I   F+G KGP+ GTKVAKYP+CPRP+L+RGLREHT
Sbjct: 125 CQLHKFAERLSKLMCENLGLDQEDIMNAFSGPKGPAFGTKVAKYPECPRPELMRGLREHT 184

Query: 189 DAGGIILLLQDDQVPGLEFENDGVWFKIPPSKNNAIFVNIGDQIEVLSSGKYKSILHRVL 248
           DAGGIILLLQDDQVPGLEF  DG W  IPPSKNN IFVN GDQ+E+LS+G+YKS++HRV+
Sbjct: 185 DAGGIILLLQDDQVPGLEFFKDGKWVPIPPSKNNTIFVNTGDQLEILSNGRYKSVVHRVM 244

Query: 249 AEKDGSRLSIATFYNPATDAVISPAAELLYPGGYRFEDYLKFYNTTKFGDKEKRFQSMKR 308
             K GSRLSIATFYNPA DA+ISPA +LLYP GYRF+DYLK Y+TTKFGDK  R ++MK+
Sbjct: 245 TVKHGSRLSIATFYNPAGDAIISPAPKLLYPSGYRFQDYLKLYSTTKFGDKGPRLETMKK 304

Query: 309 M 310
           M
Sbjct: 305 M 304

BLAST of Spg003402 vs. TAIR 10
Match: AT1G62380.1 (ACC oxidase 2 )

HSP 1 Score: 313.9 bits (803), Expect = 1.4e-85
Identity = 143/304 (47.04%), Postives = 222/304 (73.03%), Query Frame = 0

Query: 11  KNMKIPTIDFNQLNGDNRAKTLSVLHDACKDWGFFLVENHGIEEEVLEKVKLLVNRHYDE 70
           KNMK P +D ++LNG+ R +T++++++AC++WGFF + NHG+  ++++K++ +   HY  
Sbjct: 3   KNMKFPVVDLSKLNGEERDQTMALINEACENWGFFEIVNHGLPHDLMDKIEKMTKDHYKT 62

Query: 71  NMKEKFYNSDVAKNLGKM--AVSDVDWESTFFIWHKPNSNISQFTNVSQDLCKAMEEEYI 130
             ++KF +   +K L  +   V DVDWESTF++ H P SN++  ++VS +   AM +++ 
Sbjct: 63  CQEQKFNDMLKSKGLDNLETEVEDVDWESTFYVRHLPQSNLNDISDVSDEYRTAM-KDFG 122

Query: 131 PQVIKIAEKLAELMCENLGLEKAHIKQLFTGTKGPSVGTKVAKYPKCPRPDLVRGLREHT 190
            ++  +AE L +L+CENLGLEK ++K++F GTKGP+ GTKV+ YP CP+P++++GLR HT
Sbjct: 123 KRLENLAEDLLDLLCENLGLEKGYLKKVFHGTKGPTFGTKVSNYPPCPKPEMIKGLRAHT 182

Query: 191 DAGGIILLLQDDQVPGLEFENDGVWFKIPPSKNNAIFVNIGDQIEVLSSGKYKSILHRVL 250
           DAGGIILL QDD+V GL+   DG W  +PP  N++I +N+GDQ+EV+++GKYKS+LHRV+
Sbjct: 183 DAGGIILLFQDDKVSGLQLLKDGDWIDVPP-LNHSIVINLGDQLEVITNGKYKSVLHRVV 242

Query: 251 AEKDGSRLSIATFYNPATDAVISPAAELL-----YPGGYRFEDYLKFYNTTKFGDKEKRF 308
            +++G+R+S+A+FYNP +DA ISPA  L+     YP  + F+DY+K Y   KF  KE RF
Sbjct: 243 TQQEGNRMSVASFYNPGSDAEISPATSLVEKDSEYP-SFVFDDYMKLYAGVKFQPKEPRF 302

BLAST of Spg003402 vs. TAIR 10
Match: AT1G05010.1 (ethylene-forming enzyme )

HSP 1 Score: 303.5 bits (776), Expect = 1.9e-82
Identity = 153/310 (49.35%), Postives = 216/310 (69.68%), Query Frame = 0

Query: 16  PTIDFNQLNGDNRAKTLSVLHDACKDWGFFLVENHGIEEEVLEKVKLLVNRHYDENMKEK 75
           P I+  +LNG+ RA T+  + DAC++WGFF   NHGI  E+L+KV+ +   HY + M+E+
Sbjct: 5   PIINLEKLNGEERAITMEKIKDACENWGFFECVNHGISLELLDKVEKMTKEHYKKCMEER 64

Query: 76  FYNSDVAKNLG----KMAVSDVDWESTFFIWHKPNSNISQFTNVSQDLCKAMEEEYIPQV 135
           F  S   KN G    +  V+DVDWESTF++ H P SNIS   ++  D  + + +++  ++
Sbjct: 65  FKES--IKNRGLDSLRSEVNDVDWESTFYLKHLPVSNISDVPDLDDDY-RTLMKDFAGKI 124

Query: 136 IKIAEKLAELMCENLGLEKAHIKQLFTGTKGPSVGTKVAKYPKCPRPDLVRGLREHTDAG 195
            K++E+L +L+CENLGLEK ++K++F G+K P+ GTKV+ YP CP PDLV+GLR HTDAG
Sbjct: 125 EKLSEELLDLLCENLGLEKGYLKKVFYGSKRPTFGTKVSNYPPCPNPDLVKGLRAHTDAG 184

Query: 196 GIILLLQDDQVPGLEFENDGVWFKIPPSKNNAIFVNIGDQIEVLSSGKYKSILHRVLAEK 255
           GIILL QDD+V GL+   DG W  +PP K ++I VN+GDQ+EV+++GKYKS+ HRVL++ 
Sbjct: 185 GIILLFQDDKVSGLQLLKDGEWVDVPPVK-HSIVVNLGDQLEVITNGKYKSVEHRVLSQT 244

Query: 256 DG-SRLSIATFYNPATDAVISPAAELL-----------YPGGYRFEDYLKFYNTTKFGDK 310
           DG  R+SIA+FYNP +D+VI PA EL+           YP  + FEDY+K Y+  KF  K
Sbjct: 245 DGEGRMSIASFYNPGSDSVIFPAPELIGKEAEKEKKENYP-RFVFEDYMKLYSAVKFQAK 304

BLAST of Spg003402 vs. TAIR 10
Match: AT1G12010.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 303.1 bits (775), Expect = 2.5e-82
Identity = 139/303 (45.87%), Postives = 219/303 (72.28%), Query Frame = 0

Query: 12  NMKIPTIDFNQLNGDNRAKTLSVLHDACKDWGFFLVENHGIEEEVLEKVKLLVNRHYDEN 71
           N+K P ID ++LNG+ R +T++++ DAC++WGFF + NHG+  ++++ ++ +   HY ++
Sbjct: 4   NIKFPVIDLSKLNGEERDQTMALIDDACQNWGFFELVNHGLPYDLMDNIERMTKEHYKKH 63

Query: 72  MKEKFYNSDVAKNLGKM--AVSDVDWESTFFIWHKPNSNISQFTNVSQDLCKAMEEEYIP 131
           M++KF     +K L  +   V DVDWESTF++ H P SN+    ++S +   AM +++  
Sbjct: 64  MEQKFKEMLRSKGLDTLETEVEDVDWESTFYLHHLPQSNLYDIPDMSNEYRLAM-KDFGK 123

Query: 132 QVIKIAEKLAELMCENLGLEKAHIKQLFTGTKGPSVGTKVAKYPKCPRPDLVRGLREHTD 191
           ++  +AE+L +L+CENLGLEK ++K++F GT GP+  TK++ YP CP+P++++GLR HTD
Sbjct: 124 RLEILAEELLDLLCENLGLEKGYLKKVFHGTTGPTFATKLSNYPPCPKPEMIKGLRAHTD 183

Query: 192 AGGIILLLQDDQVPGLEFENDGVWFKIPPSKNNAIFVNIGDQIEVLSSGKYKSILHRVLA 251
           AGG+ILL QDD+V GL+   DG W  +PP K ++I +N+GDQ+EV+++GKYKS++HRV+ 
Sbjct: 184 AGGLILLFQDDKVSGLQLLKDGDWVDVPPLK-HSIVINLGDQLEVITNGKYKSVMHRVMT 243

Query: 252 EKDGSRLSIATFYNPATDAVISPAAELL-----YPGGYRFEDYLKFYNTTKFGDKEKRFQ 308
           +K+G+R+SIA+FYNP +DA ISPA  L+     YP  + F+DY+K Y   KF  KE RF+
Sbjct: 244 QKEGNRMSIASFYNPGSDAEISPATSLVDKDSKYP-SFVFDDYMKLYAGLKFQAKEPRFE 303

BLAST of Spg003402 vs. TAIR 10
Match: AT1G77330.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 246.1 bits (627), Expect = 3.6e-65
Identity = 143/317 (45.11%), Postives = 186/317 (58.68%), Query Frame = 0

Query: 13  MKIPTIDFNQLNGDNRAKTLSVLHDACKDWGFFLVENHGIEEEVLEKVKLLVNRHYDENM 72
           M IP IDF++LNG+ R KTLS +  AC++WGFF + NHGI  E+L KVK L +  Y    
Sbjct: 1   MAIPVIDFSKLNGEEREKTLSEIARACEEWGFFQLVNHGIPLELLNKVKKLSSDCYKTER 60

Query: 73  KEKFYNSDVAKNLGKMA-------VSDVDWESTF-FIWHKPNSNISQFTNVSQDLCKAME 132
           +E F  S+  K L ++        + +VDWE  F  + H  N   S          K   
Sbjct: 61  EEAFKTSNPVKLLNELVQKNSGEKLENVDWEDVFTLLDHNQNEWPSNI--------KETM 120

Query: 133 EEYIPQVIKIAEKLAELMCENLGLEKAHIKQLFT-----GTKGPSVGTKVAKYPKCPRPD 192
            EY  +V K+A K+ E+M ENLGL K +IK+ F      G +    GTKV+ YP CP P+
Sbjct: 121 GEYREEVRKLASKMMEVMDENLGLPKGYIKKAFNEGMEDGEETAFFGTKVSHYPPCPHPE 180

Query: 193 LVRGLREHTDAGGIILLLQDDQVPGLEFENDGVWFKIPPSKNNAIFVNIGDQIEVLSSGK 252
           LV GLR HTDAGG++LL QDD+  GL+   DG W  + P   NAI +N GDQIEVLS+G+
Sbjct: 181 LVNGLRAHTDAGGVVLLFQDDEYDGLQVLKDGEWIDVQPLP-NAIVINTGDQIEVLSNGR 240

Query: 253 YKSILHRVLAEKDGSRLSIATFYNPATDAVISPAA-------ELLYPGGYRFEDYLKFYN 310
           YKS  HRVLA ++G+R SIA+FYNP+  A I PAA       E  YP  + F DY+  Y 
Sbjct: 241 YKSAWHRVLAREEGNRRSIASFYNPSYKAAIGPAAVAEEEGSEKKYP-KFVFGDYMDVYA 300