Spg003327 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg003327 vs. NCBI nr
Match: XP_023546787.1 (GDP-fucose transporter 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 620.5 bits (1599), Expect = 8.4e-174
Identity = 319/341 (93.55%), Postives = 329/341 (96.48%), Query Frame = 0

Query: 1   MASIRLDSKQYFTTSGLVVGYALCSSLLAVINKYAITKFNYPGLLTALQYLTSAVGVWIL 60
           M+SIRLDSKQYFTTSGLV+GYALCSSLLAVINK+AITKFNYPGLLTALQYLTSAVGVW+L
Sbjct: 1   MSSIRLDSKQYFTTSGLVIGYALCSSLLAVINKFAITKFNYPGLLTALQYLTSAVGVWVL 60

Query: 61  GKLGFLHHDPFIYSTAKKFLPAAFVFYLAIFTNTNLLRHANVDTFIVFRSCTPLLVAIAD 120
           GKLGFLHHDPFIY+TAKKFLPAAFVFYLAIFTNTNLLRHANVDTFIVFRSCTPLLVAIAD
Sbjct: 61  GKLGFLHHDPFIYATAKKFLPAAFVFYLAIFTNTNLLRHANVDTFIVFRSCTPLLVAIAD 120

Query: 121 TAFRNQPCPSKLTFVSLLIILGGAVGYVVTDSAFTLTAYSWAFAYLVTITTEMVYIKHMV 180
           TAFR+QPCPSKLTF SLLIILGGAVGYV TDSAFTLTAYSWAFAYLVTITTEMVYIKHMV
Sbjct: 121 TAFRHQPCPSKLTFGSLLIILGGAVGYVATDSAFTLTAYSWAFAYLVTITTEMVYIKHMV 180

Query: 181 TNLGLNTWGFVLYNNLISLILAPAFWFITGEYADVFAALGSSGGDWFQYDAFYAVSLSCV 240
           TNLGLNTWGFVLYNNLISLILAPAFWFITGEY DVF+ALGSS GDWFQYDAFYAVSLSCV
Sbjct: 181 TNLGLNTWGFVLYNNLISLILAPAFWFITGEYVDVFSALGSSKGDWFQYDAFYAVSLSCV 240

Query: 241 FGFLISFFGFAARKAISATAFTVTGVVNKFLTVAINVMIWDKHANPFGLVCLLFTISGGV 300
           FGFLISFFGFAARKAISATAFTVTGVVNKFLTVAINV+IWDKHANPFGLVCLLFTISGGV
Sbjct: 241 FGFLISFFGFAARKAISATAFTVTGVVNKFLTVAINVVIWDKHANPFGLVCLLFTISGGV 300

Query: 301 LYQQSVTGVGSASSTPAKPTENENDLEENQGKGVPGKLSSV 342
           LYQQSV+GVGSA ST  KPT N+ND EEN GK  PGKLSSV
Sbjct: 301 LYQQSVSGVGSAPSTADKPTGNDNDPEENHGK--PGKLSSV 339

BLAST of Spg003327 vs. NCBI nr
Match: XP_022946347.1 (GDP-fucose transporter 1 [Cucurbita moschata])

HSP 1 Score: 619.8 bits (1597), Expect = 1.4e-173
Identity = 318/341 (93.26%), Postives = 330/341 (96.77%), Query Frame = 0

Query: 1   MASIRLDSKQYFTTSGLVVGYALCSSLLAVINKYAITKFNYPGLLTALQYLTSAVGVWIL 60
           M+SIRLDSKQYFTTSGLV+GYALCSSLLAVINK+AITKFNYPGLLTALQYLTSAVGVW+L
Sbjct: 1   MSSIRLDSKQYFTTSGLVIGYALCSSLLAVINKFAITKFNYPGLLTALQYLTSAVGVWVL 60

Query: 61  GKLGFLHHDPFIYSTAKKFLPAAFVFYLAIFTNTNLLRHANVDTFIVFRSCTPLLVAIAD 120
           GKLGFLHHDPFIY+TAKKFLPAAFVFYLAIFTNTNLLRHANVDTFIVFRSCTPLLVAIAD
Sbjct: 61  GKLGFLHHDPFIYATAKKFLPAAFVFYLAIFTNTNLLRHANVDTFIVFRSCTPLLVAIAD 120

Query: 121 TAFRNQPCPSKLTFVSLLIILGGAVGYVVTDSAFTLTAYSWAFAYLVTITTEMVYIKHMV 180
           TAFR+QPCPSKLTF SLLIILGGAVGYV TDSAFTLTAYSWAFAYLVTITTEMVYIKHMV
Sbjct: 121 TAFRHQPCPSKLTFGSLLIILGGAVGYVATDSAFTLTAYSWAFAYLVTITTEMVYIKHMV 180

Query: 181 TNLGLNTWGFVLYNNLISLILAPAFWFITGEYADVFAALGSSGGDWFQYDAFYAVSLSCV 240
           TNLGLNTWGFVLYNNLISLILAPAFWFITGEY DVF+ALGSS GDWFQYDAFYAVSLSCV
Sbjct: 181 TNLGLNTWGFVLYNNLISLILAPAFWFITGEYVDVFSALGSSKGDWFQYDAFYAVSLSCV 240

Query: 241 FGFLISFFGFAARKAISATAFTVTGVVNKFLTVAINVMIWDKHANPFGLVCLLFTISGGV 300
           FGFLISFFGFAARKAISATAFTVTGVVNKFLTVAINV+IWDKHANPFGLVCLLFTISGGV
Sbjct: 241 FGFLISFFGFAARKAISATAFTVTGVVNKFLTVAINVVIWDKHANPFGLVCLLFTISGGV 300

Query: 301 LYQQSVTGVGSASSTPAKPTENENDLEENQGKGVPGKLSSV 342
           LYQQSV+GVGSA ST  KPT N+ND E+N+GK  PGKLSSV
Sbjct: 301 LYQQSVSGVGSAPSTADKPTGNDNDPEDNRGK--PGKLSSV 339

BLAST of Spg003327 vs. NCBI nr
Match: XP_022999367.1 (GDP-fucose transporter 1 [Cucurbita maxima])

HSP 1 Score: 619.4 bits (1596), Expect = 1.9e-173
Identity = 319/341 (93.55%), Postives = 328/341 (96.19%), Query Frame = 0

Query: 1   MASIRLDSKQYFTTSGLVVGYALCSSLLAVINKYAITKFNYPGLLTALQYLTSAVGVWIL 60
           M+SIRLDSKQYFTTSGLV+GYALCSSLLAVINK+AITKFNYPGLLTALQYLTSAVGVW+L
Sbjct: 1   MSSIRLDSKQYFTTSGLVIGYALCSSLLAVINKFAITKFNYPGLLTALQYLTSAVGVWVL 60

Query: 61  GKLGFLHHDPFIYSTAKKFLPAAFVFYLAIFTNTNLLRHANVDTFIVFRSCTPLLVAIAD 120
           GKLGFLHHDPFIY+TAKKFLPAAFVFYLAIFTNTNLLRHANVDTFIVFRSCTPLLVAIAD
Sbjct: 61  GKLGFLHHDPFIYATAKKFLPAAFVFYLAIFTNTNLLRHANVDTFIVFRSCTPLLVAIAD 120

Query: 121 TAFRNQPCPSKLTFVSLLIILGGAVGYVVTDSAFTLTAYSWAFAYLVTITTEMVYIKHMV 180
           TAFR QPCPSKLTF SLLIILGGAVGYV TDSAFTLTAYSWAFAYLVTITTEMVYIKHMV
Sbjct: 121 TAFRYQPCPSKLTFGSLLIILGGAVGYVATDSAFTLTAYSWAFAYLVTITTEMVYIKHMV 180

Query: 181 TNLGLNTWGFVLYNNLISLILAPAFWFITGEYADVFAALGSSGGDWFQYDAFYAVSLSCV 240
           TNLGLNTWGFVLYNNLISLILAPAFWFITGEY DVF+ALGSS GDWFQYDAFYAVSLSCV
Sbjct: 181 TNLGLNTWGFVLYNNLISLILAPAFWFITGEYVDVFSALGSSKGDWFQYDAFYAVSLSCV 240

Query: 241 FGFLISFFGFAARKAISATAFTVTGVVNKFLTVAINVMIWDKHANPFGLVCLLFTISGGV 300
           FGFLISFFGFAARKAISATAFTVTGVVNKFLTVAINV+IWDKHANPFGLVCLLFTISGGV
Sbjct: 241 FGFLISFFGFAARKAISATAFTVTGVVNKFLTVAINVVIWDKHANPFGLVCLLFTISGGV 300

Query: 301 LYQQSVTGVGSASSTPAKPTENENDLEENQGKGVPGKLSSV 342
           LYQQSV+GVGSA ST  KPT N+ND EEN GK  PGKLSSV
Sbjct: 301 LYQQSVSGVGSAPSTADKPTGNDNDPEENHGK--PGKLSSV 339

BLAST of Spg003327 vs. NCBI nr
Match: KAG6599419.1 (GDP-fucose transporter 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 613.6 bits (1581), Expect = 1.0e-171
Identity = 313/334 (93.71%), Postives = 324/334 (97.01%), Query Frame = 0

Query: 1   MASIRLDSKQYFTTSGLVVGYALCSSLLAVINKYAITKFNYPGLLTALQYLTSAVGVWIL 60
           M+SIRLDSKQYFTTSGLV+GYALCSSLLAVINK+AITKFNYPGLLTALQYLTSAVGVW+L
Sbjct: 1   MSSIRLDSKQYFTTSGLVIGYALCSSLLAVINKFAITKFNYPGLLTALQYLTSAVGVWVL 60

Query: 61  GKLGFLHHDPFIYSTAKKFLPAAFVFYLAIFTNTNLLRHANVDTFIVFRSCTPLLVAIAD 120
           GKLGFLHHDPFIY+TAKKFLPAAFVFYLAIFTNTNLLRHANVDTFIVFRSCTPLLVAIAD
Sbjct: 61  GKLGFLHHDPFIYATAKKFLPAAFVFYLAIFTNTNLLRHANVDTFIVFRSCTPLLVAIAD 120

Query: 121 TAFRNQPCPSKLTFVSLLIILGGAVGYVVTDSAFTLTAYSWAFAYLVTITTEMVYIKHMV 180
           TAFR+QPCPSKLTF SLLIILGGAVGYV TDSAFTLTAYSWAFAYLVTITTEMVYIKHMV
Sbjct: 121 TAFRHQPCPSKLTFGSLLIILGGAVGYVATDSAFTLTAYSWAFAYLVTITTEMVYIKHMV 180

Query: 181 TNLGLNTWGFVLYNNLISLILAPAFWFITGEYADVFAALGSSGGDWFQYDAFYAVSLSCV 240
           TNLGLNTWGFVLYNNLISLILAPAFWFITGEY DVF+ALGSS GDWFQYDAFYAVSLSCV
Sbjct: 181 TNLGLNTWGFVLYNNLISLILAPAFWFITGEYVDVFSALGSSKGDWFQYDAFYAVSLSCV 240

Query: 241 FGFLISFFGFAARKAISATAFTVTGVVNKFLTVAINVMIWDKHANPFGLVCLLFTISGGV 300
           FGFLISFFGFAARKAISATAFTVTGVVNKFLTVAINV+IWDKHANPFGLVCLLFTISGGV
Sbjct: 241 FGFLISFFGFAARKAISATAFTVTGVVNKFLTVAINVVIWDKHANPFGLVCLLFTISGGV 300

Query: 301 LYQQSVTGVGSASSTPAKPTENENDLEENQGKGV 335
           LYQQSV+GVGSA ST  KPT N+ND EEN+GK V
Sbjct: 301 LYQQSVSGVGSAPSTADKPTGNDNDPEENRGKPV 334

BLAST of Spg003327 vs. NCBI nr
Match: KAG7030409.1 (GDP-fucose transporter 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 610.9 bits (1574), Expect = 6.6e-171
Identity = 312/334 (93.41%), Postives = 323/334 (96.71%), Query Frame = 0

Query: 1   MASIRLDSKQYFTTSGLVVGYALCSSLLAVINKYAITKFNYPGLLTALQYLTSAVGVWIL 60
           M+SIRLDSKQYFTTSGLV+GYALCSSLLAVINK+AITKFNYPGLLTALQYLTSAVGVW+L
Sbjct: 1   MSSIRLDSKQYFTTSGLVIGYALCSSLLAVINKFAITKFNYPGLLTALQYLTSAVGVWVL 60

Query: 61  GKLGFLHHDPFIYSTAKKFLPAAFVFYLAIFTNTNLLRHANVDTFIVFRSCTPLLVAIAD 120
           GKLGFLHHDPFIY+TAKKFLPAAFVFYLAIFTNTNLLRHANVDTFIVFRSCTPLLVAIAD
Sbjct: 61  GKLGFLHHDPFIYATAKKFLPAAFVFYLAIFTNTNLLRHANVDTFIVFRSCTPLLVAIAD 120

Query: 121 TAFRNQPCPSKLTFVSLLIILGGAVGYVVTDSAFTLTAYSWAFAYLVTITTEMVYIKHMV 180
           TAFR+QPCPSKLTF SLLIILGGAVGYV TDSAFTLTAYSWAFAYLVTITTEMVYIKHMV
Sbjct: 121 TAFRHQPCPSKLTFGSLLIILGGAVGYVATDSAFTLTAYSWAFAYLVTITTEMVYIKHMV 180

Query: 181 TNLGLNTWGFVLYNNLISLILAPAFWFITGEYADVFAALGSSGGDWFQYDAFYAVSLSCV 240
           TNLGLNTWGFVLYNNLISLILAPAFWFITGEY DVF+AL SS GDWFQYDAFYAVSLSCV
Sbjct: 181 TNLGLNTWGFVLYNNLISLILAPAFWFITGEYVDVFSALDSSKGDWFQYDAFYAVSLSCV 240

Query: 241 FGFLISFFGFAARKAISATAFTVTGVVNKFLTVAINVMIWDKHANPFGLVCLLFTISGGV 300
           FGFLISFFGFAARKAISATAFTVTGVVNKFLTVAINV+IWDKHANPFGLVCLLFTISGGV
Sbjct: 241 FGFLISFFGFAARKAISATAFTVTGVVNKFLTVAINVVIWDKHANPFGLVCLLFTISGGV 300

Query: 301 LYQQSVTGVGSASSTPAKPTENENDLEENQGKGV 335
           LYQQSV+GVGSA ST  KPT N+ND EEN+GK V
Sbjct: 301 LYQQSVSGVGSAPSTADKPTGNDNDPEENRGKPV 334

BLAST of Spg003327 vs. ExPASy Swiss-Prot
Match: Q84L08 (GDP-fucose transporter 1 OS=Arabidopsis thaliana OX=3702 GN=GFT1 PE=1 SV=1)

HSP 1 Score: 466.8 bits (1200), Expect = 2.0e-130
Identity = 246/341 (72.14%), Postives = 281/341 (82.40%), Query Frame = 0

Query: 1   MASIRLDSKQYFTTSGLVVGYALCSSLLAVINKYAITKFNYPGLLTALQYLTSAVGVWIL 60
           M+S R DS +  TTS LV+GYALCSSLLAVINK AIT FNYPGLLTALQYLT  V V++L
Sbjct: 1   MSSSRFDSNKQLTTSSLVIGYALCSSLLAVINKLAITYFNYPGLLTALQYLTCTVAVYLL 60

Query: 61  GKLGFLHHDPFIYSTAKKFLPAAFVFYLAIFTNTNLLRHANVDTFIVFRSCTPLLVAIAD 120
           GK G ++HDPF + TAKKFLPAA VFYLAIFTNTNLLRHANVDTFIVFRS TPLLVAIAD
Sbjct: 61  GKSGLINHDPFTWDTAKKFLPAAIVFYLAIFTNTNLLRHANVDTFIVFRSLTPLLVAIAD 120

Query: 121 TAFRNQPCPSKLTFVSLLIILGGAVGYVVTDSAFTLTAYSWAFAYLVTITTEMVYIKHMV 180
           T FR+QP PS+LTF+SL++IL GAVGYV TDS+FTLTAYSWA AYLVTITTEMVYIKHMV
Sbjct: 121 TVFRSQPLPSRLTFLSLVVILAGAVGYVATDSSFTLTAYSWALAYLVTITTEMVYIKHMV 180

Query: 181 TNLGLNTWGFVLYNNLISLILAPAFWFITGEYADVFAALGSSGGDWFQYDAFYAVSLSCV 240
           +N+ LN WG VLYNNL+SL++AP FWF+TGE+ +VFAAL  + G+ F+  AF +V+ SCV
Sbjct: 181 SNIKLNIWGLVLYNNLLSLMIAPVFWFLTGEFTEVFAALSENRGNLFEPYAFSSVAASCV 240

Query: 241 FGFLISFFGFAARKAISATAFTVTGVVNKFLTVAINVMIWDKHANPFGLVCLLFTISGGV 300
           FGFLIS+FGFAAR AISATAFTVTGVVNKFLTV INV+IWDKHA P GLVCLLFTI GGV
Sbjct: 241 FGFLISYFGFAARNAISATAFTVTGVVNKFLTVVINVLIWDKHATPVGLVCLLFTICGGV 300

Query: 301 LYQQSVTGVGSASSTPAKPTENENDLEENQGKGVPGKLSSV 342
            YQQSV           K +E + + +E   + VPGKL+SV
Sbjct: 301 GYQQSVKLDKPIEKVSEKDSE-KGEEDEELTQLVPGKLASV 340

BLAST of Spg003327 vs. ExPASy Swiss-Prot
Match: Q9S845 (GDP-mannose transporter GONST3 OS=Arabidopsis thaliana OX=3702 GN=GONST3 PE=2 SV=1)

HSP 1 Score: 303.5 bits (776), Expect = 3.0e-81
Identity = 164/313 (52.40%), Postives = 212/313 (67.73%), Query Frame = 0

Query: 16  GLVVGYALCSSLLAVINKYAITKFNYPGLLTALQYLTSAVGVWILGKLGFLHHDPFIYST 75
           G+  GY L +SLL++INK+AI KF YPG LTA+QY TSA GV +  ++  + HD     T
Sbjct: 37  GVAAGYCLSASLLSIINKWAIMKFPYPGALTAMQYFTSAAGVLLCAQMKLIEHDSLNLLT 96

Query: 76  AKKFLPAAFVFYLAIFTNTNLLRHANVDTFIVFRSCTPLLVAIADTAFRNQPCPSKLTFV 135
             +FLPAA +FYL++FTN+ LL HANVDTFIVFRS  P+ VAI +T F +QP PS  T+ 
Sbjct: 97  MWRFLPAAMIFYLSLFTNSELLLHANVDTFIVFRSAVPIFVAIGETLFLHQPWPSVKTWG 156

Query: 136 SLLIILGGAVGYVVTDSAFTLTAYSWAFAYLVTITTEMVYIKHMVTNLGLNTWGFVLYNN 195
           SL  I GG++ YV TD  FT+ AYSWA AYLV++T + VYIKH+V  +GLNTWG VLYNN
Sbjct: 157 SLATIFGGSLLYVFTDYQFTIAAYSWALAYLVSMTIDFVYIKHVVMTIGLNTWGLVLYNN 216

Query: 196 LISLILAPAFWFITGEYADVFAALGSSGGDWFQYDAFYAVSLSCVFGFLISFFGFAARKA 255
           L +L+L P    I GE   +   + +   DW+       V LSC+FG  ISFFGF+ R+A
Sbjct: 217 LEALLLFPLELLIMGELKKIKHEI-TDETDWYSLQVVLPVGLSCLFGLAISFFGFSCRRA 276

Query: 256 ISATAFTVTGVVNKFLTVAINVMIWDKHANPFGLVCLLFTISGGVLYQQSVTGVGSASST 315
           ISAT FTV G+VNK LTV IN+M+WDKH+   G + LL  + GGV+YQQS     +A+  
Sbjct: 277 ISATGFTVLGIVNKLLTVVINLMVWDKHSTFVGTLGLLVCMFGGVMYQQSTIKKPNATQ- 336

Query: 316 PAKPTENENDLEE 329
            AKP E + + E+
Sbjct: 337 EAKPQEQDEEQEK 347

BLAST of Spg003327 vs. ExPASy Swiss-Prot
Match: A5DIN8 (GDP-mannose transporter OS=Meyerozyma guilliermondii (strain ATCC 6260 / CBS 566 / DSM 6381 / JCM 1539 / NBRC 10279 / NRRL Y-324) OX=294746 GN=VRG4 PE=3 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 3.0e-09
Identity = 65/297 (21.89%), Postives = 129/297 (43.43%), Query Frame = 0

Query: 18  VVGYALCSSLLAVINKYAI--TKFNYPGLLTALQYLTSAVGVWILGKLGFLHHDPFIYST 77
           +  Y   S L+ V NK+ +   +FN    L A+Q     V +  L  LG + +  F    
Sbjct: 40  IFAYCASSILMTVTNKFVVGAYEFNLNFFLLAVQAAVCLVTIATLKGLGIITYRQFNKDE 99

Query: 78  AKKFLPAAFVFYLAIFTNTNLLRHANVDTFIVFRSCTPLLVAIADTAFRNQPCPSKLTFV 137
           AKK+ P AF+  L I+T++  L++ ++  + +F++ T +L+A  +  +      +     
Sbjct: 100 AKKWFPIAFLLVLMIYTSSKALQYLSIPVYTIFKNLTIILIAYGEVIWFGGKVTTMALGS 159

Query: 138 SLLIILGGAVGYV---------VTDSAFTLTAYSWAFAYLVTITTEMVYIKHMVTNLGLN 197
            +L++L   + Y            +       Y+W F    +    ++ ++  +      
Sbjct: 160 FILMVLSSVIAYYGDTAETGEKTAEMHLLYLGYAWMFTNCFSSAAFVLIMRKRIKLTNFK 219

Query: 198 TWGFVLYNNLISLILAPAFWFITGEYADVFAALGSSGGDWFQYDAFYAVSLSCVFGFLIS 257
            +  + YNNL+SL L   F F+  +++ V   L  +     +    + + LS      IS
Sbjct: 220 DFDTMYYNNLLSLPLLLVFSFLFEDWSSV--NLNKNFPPDNRNTTIFVMILSGASSVGIS 279

Query: 258 FFGFAARKAISATAFTVTGVVNKFLTVAINVMIWDKHANPFGLVCLLFT-ISGGVLY 303
           +      +  S+T +++ G +NK L +A++ +++   A  F  V  +F     GV Y
Sbjct: 280 YCSAWCVRVTSSTTYSMVGALNK-LPIALSGLVFFNAAVNFWSVSSIFVGFLAGVFY 333

BLAST of Spg003327 vs. ExPASy TrEMBL
Match: A0A6J1G3H1 (GDP-fucose transporter 1 OS=Cucurbita moschata OX=3662 GN=LOC111450446 PE=4 SV=1)

HSP 1 Score: 619.8 bits (1597), Expect = 6.9e-174
Identity = 318/341 (93.26%), Postives = 330/341 (96.77%), Query Frame = 0

Query: 1   MASIRLDSKQYFTTSGLVVGYALCSSLLAVINKYAITKFNYPGLLTALQYLTSAVGVWIL 60
           M+SIRLDSKQYFTTSGLV+GYALCSSLLAVINK+AITKFNYPGLLTALQYLTSAVGVW+L
Sbjct: 1   MSSIRLDSKQYFTTSGLVIGYALCSSLLAVINKFAITKFNYPGLLTALQYLTSAVGVWVL 60

Query: 61  GKLGFLHHDPFIYSTAKKFLPAAFVFYLAIFTNTNLLRHANVDTFIVFRSCTPLLVAIAD 120
           GKLGFLHHDPFIY+TAKKFLPAAFVFYLAIFTNTNLLRHANVDTFIVFRSCTPLLVAIAD
Sbjct: 61  GKLGFLHHDPFIYATAKKFLPAAFVFYLAIFTNTNLLRHANVDTFIVFRSCTPLLVAIAD 120

Query: 121 TAFRNQPCPSKLTFVSLLIILGGAVGYVVTDSAFTLTAYSWAFAYLVTITTEMVYIKHMV 180
           TAFR+QPCPSKLTF SLLIILGGAVGYV TDSAFTLTAYSWAFAYLVTITTEMVYIKHMV
Sbjct: 121 TAFRHQPCPSKLTFGSLLIILGGAVGYVATDSAFTLTAYSWAFAYLVTITTEMVYIKHMV 180

Query: 181 TNLGLNTWGFVLYNNLISLILAPAFWFITGEYADVFAALGSSGGDWFQYDAFYAVSLSCV 240
           TNLGLNTWGFVLYNNLISLILAPAFWFITGEY DVF+ALGSS GDWFQYDAFYAVSLSCV
Sbjct: 181 TNLGLNTWGFVLYNNLISLILAPAFWFITGEYVDVFSALGSSKGDWFQYDAFYAVSLSCV 240

Query: 241 FGFLISFFGFAARKAISATAFTVTGVVNKFLTVAINVMIWDKHANPFGLVCLLFTISGGV 300
           FGFLISFFGFAARKAISATAFTVTGVVNKFLTVAINV+IWDKHANPFGLVCLLFTISGGV
Sbjct: 241 FGFLISFFGFAARKAISATAFTVTGVVNKFLTVAINVVIWDKHANPFGLVCLLFTISGGV 300

Query: 301 LYQQSVTGVGSASSTPAKPTENENDLEENQGKGVPGKLSSV 342
           LYQQSV+GVGSA ST  KPT N+ND E+N+GK  PGKLSSV
Sbjct: 301 LYQQSVSGVGSAPSTADKPTGNDNDPEDNRGK--PGKLSSV 339

BLAST of Spg003327 vs. ExPASy TrEMBL
Match: A0A6J1KAP4 (GDP-fucose transporter 1 OS=Cucurbita maxima OX=3661 GN=LOC111493761 PE=4 SV=1)

HSP 1 Score: 619.4 bits (1596), Expect = 9.0e-174
Identity = 319/341 (93.55%), Postives = 328/341 (96.19%), Query Frame = 0

Query: 1   MASIRLDSKQYFTTSGLVVGYALCSSLLAVINKYAITKFNYPGLLTALQYLTSAVGVWIL 60
           M+SIRLDSKQYFTTSGLV+GYALCSSLLAVINK+AITKFNYPGLLTALQYLTSAVGVW+L
Sbjct: 1   MSSIRLDSKQYFTTSGLVIGYALCSSLLAVINKFAITKFNYPGLLTALQYLTSAVGVWVL 60

Query: 61  GKLGFLHHDPFIYSTAKKFLPAAFVFYLAIFTNTNLLRHANVDTFIVFRSCTPLLVAIAD 120
           GKLGFLHHDPFIY+TAKKFLPAAFVFYLAIFTNTNLLRHANVDTFIVFRSCTPLLVAIAD
Sbjct: 61  GKLGFLHHDPFIYATAKKFLPAAFVFYLAIFTNTNLLRHANVDTFIVFRSCTPLLVAIAD 120

Query: 121 TAFRNQPCPSKLTFVSLLIILGGAVGYVVTDSAFTLTAYSWAFAYLVTITTEMVYIKHMV 180
           TAFR QPCPSKLTF SLLIILGGAVGYV TDSAFTLTAYSWAFAYLVTITTEMVYIKHMV
Sbjct: 121 TAFRYQPCPSKLTFGSLLIILGGAVGYVATDSAFTLTAYSWAFAYLVTITTEMVYIKHMV 180

Query: 181 TNLGLNTWGFVLYNNLISLILAPAFWFITGEYADVFAALGSSGGDWFQYDAFYAVSLSCV 240
           TNLGLNTWGFVLYNNLISLILAPAFWFITGEY DVF+ALGSS GDWFQYDAFYAVSLSCV
Sbjct: 181 TNLGLNTWGFVLYNNLISLILAPAFWFITGEYVDVFSALGSSKGDWFQYDAFYAVSLSCV 240

Query: 241 FGFLISFFGFAARKAISATAFTVTGVVNKFLTVAINVMIWDKHANPFGLVCLLFTISGGV 300
           FGFLISFFGFAARKAISATAFTVTGVVNKFLTVAINV+IWDKHANPFGLVCLLFTISGGV
Sbjct: 241 FGFLISFFGFAARKAISATAFTVTGVVNKFLTVAINVVIWDKHANPFGLVCLLFTISGGV 300

Query: 301 LYQQSVTGVGSASSTPAKPTENENDLEENQGKGVPGKLSSV 342
           LYQQSV+GVGSA ST  KPT N+ND EEN GK  PGKLSSV
Sbjct: 301 LYQQSVSGVGSAPSTADKPTGNDNDPEENHGK--PGKLSSV 339

BLAST of Spg003327 vs. ExPASy TrEMBL
Match: A0A6J1CP75 (GDP-fucose transporter 1 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111013049 PE=4 SV=1)

HSP 1 Score: 608.2 bits (1567), Expect = 2.1e-170
Identity = 311/336 (92.56%), Postives = 321/336 (95.54%), Query Frame = 0

Query: 1   MASIRLDSKQYFTTSGLVVGYALCSSLLAVINKYAITKFNYPGLLTALQYLTSAVGVWIL 60
           M+S RLD+KQYFTTSGLVVGYALCSSLLAVINK+AITKFNYPGLLTALQYLTSAVGVW+L
Sbjct: 1   MSSTRLDTKQYFTTSGLVVGYALCSSLLAVINKFAITKFNYPGLLTALQYLTSAVGVWVL 60

Query: 61  GKLGFLHHDPFIYSTAKKFLPAAFVFYLAIFTNTNLLRHANVDTFIVFRSCTPLLVAIAD 120
           GKLGFLHHDPFIY+TAKKFLPAAFVFYLAIFTNTNLLRHANVDTFIVFRSCTPLLVAIAD
Sbjct: 61  GKLGFLHHDPFIYATAKKFLPAAFVFYLAIFTNTNLLRHANVDTFIVFRSCTPLLVAIAD 120

Query: 121 TAFRNQPCPSKLTFVSLLIILGGAVGYVVTDSAFTLTAYSWAFAYLVTITTEMVYIKHMV 180
           T FR QPCPSKLTFVSLLIILGGAVGYV TDSAFTLTAYSWAFAYLVTITTEMVYIKHMV
Sbjct: 121 TMFRKQPCPSKLTFVSLLIILGGAVGYVATDSAFTLTAYSWAFAYLVTITTEMVYIKHMV 180

Query: 181 TNLGLNTWGFVLYNNLISLILAPAFWFITGEYADVFAALGSSGGDWFQYDAFYAVSLSCV 240
           TNLGLNTWGFVLYNNLISLILAP FWFITGEYADVFAALGS+ GDWFQYDAF+AVSLSCV
Sbjct: 181 TNLGLNTWGFVLYNNLISLILAPVFWFITGEYADVFAALGSAAGDWFQYDAFFAVSLSCV 240

Query: 241 FGFLISFFGFAARKAISATAFTVTGVVNKFLTVAINVMIWDKHANPFGLVCLLFTISGGV 300
           FGFLISFFGFAARKAISATAFTVTGVVNKFLTVAINV+IWDKHANPFGLVCLLFTISGGV
Sbjct: 241 FGFLISFFGFAARKAISATAFTVTGVVNKFLTVAINVVIWDKHANPFGLVCLLFTISGGV 300

Query: 301 LYQQSVTGVGSASSTPAKPTENENDLEENQGKGVPG 337
           LYQQSVTGVG ASSTP K T +E  LEEN+GK   G
Sbjct: 301 LYQQSVTGVGVASSTPLKTTGDEKLLEENEGKASDG 336

BLAST of Spg003327 vs. ExPASy TrEMBL
Match: A0A6J1CPT0 (GDP-fucose transporter 1 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111013049 PE=4 SV=1)

HSP 1 Score: 603.6 bits (1555), Expect = 5.1e-169
Identity = 308/330 (93.33%), Postives = 318/330 (96.36%), Query Frame = 0

Query: 1   MASIRLDSKQYFTTSGLVVGYALCSSLLAVINKYAITKFNYPGLLTALQYLTSAVGVWIL 60
           M+S RLD+KQYFTTSGLVVGYALCSSLLAVINK+AITKFNYPGLLTALQYLTSAVGVW+L
Sbjct: 1   MSSTRLDTKQYFTTSGLVVGYALCSSLLAVINKFAITKFNYPGLLTALQYLTSAVGVWVL 60

Query: 61  GKLGFLHHDPFIYSTAKKFLPAAFVFYLAIFTNTNLLRHANVDTFIVFRSCTPLLVAIAD 120
           GKLGFLHHDPFIY+TAKKFLPAAFVFYLAIFTNTNLLRHANVDTFIVFRSCTPLLVAIAD
Sbjct: 61  GKLGFLHHDPFIYATAKKFLPAAFVFYLAIFTNTNLLRHANVDTFIVFRSCTPLLVAIAD 120

Query: 121 TAFRNQPCPSKLTFVSLLIILGGAVGYVVTDSAFTLTAYSWAFAYLVTITTEMVYIKHMV 180
           T FR QPCPSKLTFVSLLIILGGAVGYV TDSAFTLTAYSWAFAYLVTITTEMVYIKHMV
Sbjct: 121 TMFRKQPCPSKLTFVSLLIILGGAVGYVATDSAFTLTAYSWAFAYLVTITTEMVYIKHMV 180

Query: 181 TNLGLNTWGFVLYNNLISLILAPAFWFITGEYADVFAALGSSGGDWFQYDAFYAVSLSCV 240
           TNLGLNTWGFVLYNNLISLILAP FWFITGEYADVFAALGS+ GDWFQYDAF+AVSLSCV
Sbjct: 181 TNLGLNTWGFVLYNNLISLILAPVFWFITGEYADVFAALGSAAGDWFQYDAFFAVSLSCV 240

Query: 241 FGFLISFFGFAARKAISATAFTVTGVVNKFLTVAINVMIWDKHANPFGLVCLLFTISGGV 300
           FGFLISFFGFAARKAISATAFTVTGVVNKFLTVAINV+IWDKHANPFGLVCLLFTISGGV
Sbjct: 241 FGFLISFFGFAARKAISATAFTVTGVVNKFLTVAINVVIWDKHANPFGLVCLLFTISGGV 300

Query: 301 LYQQSVTGVGSASSTPAKPTENENDLEENQ 331
           LYQQSVTGVG ASSTP K T +E  LEEN+
Sbjct: 301 LYQQSVTGVGVASSTPLKTTGDEKLLEENE 330

BLAST of Spg003327 vs. ExPASy TrEMBL
Match: A0A1S3C698 (GDP-mannose transporter GONST4 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103497519 PE=4 SV=1)

HSP 1 Score: 602.8 bits (1553), Expect = 8.7e-169
Identity = 306/334 (91.62%), Postives = 318/334 (95.21%), Query Frame = 0

Query: 1   MASIRLDSKQYFTTSGLVVGYALCSSLLAVINKYAITKFNYPGLLTALQYLTSAVGVWIL 60
           M+SIR+D+KQYFTTSGLV+GYALCSSLLAVINK+AITKFNYPGLLTALQYLTSAVGVWIL
Sbjct: 1   MSSIRVDTKQYFTTSGLVIGYALCSSLLAVINKFAITKFNYPGLLTALQYLTSAVGVWIL 60

Query: 61  GKLGFLHHDPFIYSTAKKFLPAAFVFYLAIFTNTNLLRHANVDTFIVFRSCTPLLVAIAD 120
           GKLGFLHHDPF Y+TAKKFLPAAFVFYLAIFTNTNLLRHANVDTFIVFRSCTPLLVAIAD
Sbjct: 61  GKLGFLHHDPFTYATAKKFLPAAFVFYLAIFTNTNLLRHANVDTFIVFRSCTPLLVAIAD 120

Query: 121 TAFRNQPCPSKLTFVSLLIILGGAVGYVVTDSAFTLTAYSWAFAYLVTITTEMVYIKHMV 180
           T FRNQPCPSKLTF SLLIILGGAVGYV TDSAFTLTAYSWAFAYLVTITTEMVYIKHMV
Sbjct: 121 TMFRNQPCPSKLTFGSLLIILGGAVGYVATDSAFTLTAYSWAFAYLVTITTEMVYIKHMV 180

Query: 181 TNLGLNTWGFVLYNNLISLILAPAFWFITGEYADVFAALGSSGGDWFQYDAFYAVSLSCV 240
           TNLGLNTWGFVLYNNLISLILAP FWFITGEY DVF+ LGSSGGDWF+YDAF+AVSLSCV
Sbjct: 181 TNLGLNTWGFVLYNNLISLILAPVFWFITGEYVDVFSTLGSSGGDWFEYDAFFAVSLSCV 240

Query: 241 FGFLISFFGFAARKAISATAFTVTGVVNKFLTVAINVMIWDKHANPFGLVCLLFTISGGV 300
           FGFLISFFGFAARKAISATAFTVTGVVNKFLTVAINV+IWDKHANPFGLVCLLFTISGGV
Sbjct: 241 FGFLISFFGFAARKAISATAFTVTGVVNKFLTVAINVLIWDKHANPFGLVCLLFTISGGV 300

Query: 301 LYQQSVTGVGSASSTPAKPTENENDLEENQGKGV 335
           LYQQSVTG GSA S+  KPT N+ND E N GK V
Sbjct: 301 LYQQSVTGAGSAPSSADKPTGNDNDPEGNHGKPV 334

BLAST of Spg003327 vs. TAIR 10
Match: AT5G19980.1 (golgi nucleotide sugar transporter 4 )

HSP 1 Score: 466.8 bits (1200), Expect = 1.4e-131
Identity = 246/341 (72.14%), Postives = 281/341 (82.40%), Query Frame = 0

Query: 1   MASIRLDSKQYFTTSGLVVGYALCSSLLAVINKYAITKFNYPGLLTALQYLTSAVGVWIL 60
           M+S R DS +  TTS LV+GYALCSSLLAVINK AIT FNYPGLLTALQYLT  V V++L
Sbjct: 1   MSSSRFDSNKQLTTSSLVIGYALCSSLLAVINKLAITYFNYPGLLTALQYLTCTVAVYLL 60

Query: 61  GKLGFLHHDPFIYSTAKKFLPAAFVFYLAIFTNTNLLRHANVDTFIVFRSCTPLLVAIAD 120
           GK G ++HDPF + TAKKFLPAA VFYLAIFTNTNLLRHANVDTFIVFRS TPLLVAIAD
Sbjct: 61  GKSGLINHDPFTWDTAKKFLPAAIVFYLAIFTNTNLLRHANVDTFIVFRSLTPLLVAIAD 120

Query: 121 TAFRNQPCPSKLTFVSLLIILGGAVGYVVTDSAFTLTAYSWAFAYLVTITTEMVYIKHMV 180
           T FR+QP PS+LTF+SL++IL GAVGYV TDS+FTLTAYSWA AYLVTITTEMVYIKHMV
Sbjct: 121 TVFRSQPLPSRLTFLSLVVILAGAVGYVATDSSFTLTAYSWALAYLVTITTEMVYIKHMV 180

Query: 181 TNLGLNTWGFVLYNNLISLILAPAFWFITGEYADVFAALGSSGGDWFQYDAFYAVSLSCV 240
           +N+ LN WG VLYNNL+SL++AP FWF+TGE+ +VFAAL  + G+ F+  AF +V+ SCV
Sbjct: 181 SNIKLNIWGLVLYNNLLSLMIAPVFWFLTGEFTEVFAALSENRGNLFEPYAFSSVAASCV 240

Query: 241 FGFLISFFGFAARKAISATAFTVTGVVNKFLTVAINVMIWDKHANPFGLVCLLFTISGGV 300
           FGFLIS+FGFAAR AISATAFTVTGVVNKFLTV INV+IWDKHA P GLVCLLFTI GGV
Sbjct: 241 FGFLISYFGFAARNAISATAFTVTGVVNKFLTVVINVLIWDKHATPVGLVCLLFTICGGV 300

Query: 301 LYQQSVTGVGSASSTPAKPTENENDLEENQGKGVPGKLSSV 342
            YQQSV           K +E + + +E   + VPGKL+SV
Sbjct: 301 GYQQSVKLDKPIEKVSEKDSE-KGEEDEELTQLVPGKLASV 340

BLAST of Spg003327 vs. TAIR 10
Match: AT1G76340.1 (golgi nucleotide sugar transporter 3 )

HSP 1 Score: 303.5 bits (776), Expect = 2.1e-82
Identity = 164/313 (52.40%), Postives = 212/313 (67.73%), Query Frame = 0

Query: 16  GLVVGYALCSSLLAVINKYAITKFNYPGLLTALQYLTSAVGVWILGKLGFLHHDPFIYST 75
           G+  GY L +SLL++INK+AI KF YPG LTA+QY TSA GV +  ++  + HD     T
Sbjct: 37  GVAAGYCLSASLLSIINKWAIMKFPYPGALTAMQYFTSAAGVLLCAQMKLIEHDSLNLLT 96

Query: 76  AKKFLPAAFVFYLAIFTNTNLLRHANVDTFIVFRSCTPLLVAIADTAFRNQPCPSKLTFV 135
             +FLPAA +FYL++FTN+ LL HANVDTFIVFRS  P+ VAI +T F +QP PS  T+ 
Sbjct: 97  MWRFLPAAMIFYLSLFTNSELLLHANVDTFIVFRSAVPIFVAIGETLFLHQPWPSVKTWG 156

Query: 136 SLLIILGGAVGYVVTDSAFTLTAYSWAFAYLVTITTEMVYIKHMVTNLGLNTWGFVLYNN 195
           SL  I GG++ YV TD  FT+ AYSWA AYLV++T + VYIKH+V  +GLNTWG VLYNN
Sbjct: 157 SLATIFGGSLLYVFTDYQFTIAAYSWALAYLVSMTIDFVYIKHVVMTIGLNTWGLVLYNN 216

Query: 196 LISLILAPAFWFITGEYADVFAALGSSGGDWFQYDAFYAVSLSCVFGFLISFFGFAARKA 255
           L +L+L P    I GE   +   + +   DW+       V LSC+FG  ISFFGF+ R+A
Sbjct: 217 LEALLLFPLELLIMGELKKIKHEI-TDETDWYSLQVVLPVGLSCLFGLAISFFGFSCRRA 276

Query: 256 ISATAFTVTGVVNKFLTVAINVMIWDKHANPFGLVCLLFTISGGVLYQQSVTGVGSASST 315
           ISAT FTV G+VNK LTV IN+M+WDKH+   G + LL  + GGV+YQQS     +A+  
Sbjct: 277 ISATGFTVLGIVNKLLTVVINLMVWDKHSTFVGTLGLLVCMFGGVMYQQSTIKKPNATQ- 336

Query: 316 PAKPTENENDLEE 329
            AKP E + + E+
Sbjct: 337 EAKPQEQDEEQEK 347