Spg002279 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg002279 vs. NCBI nr
Match: XP_038877121.1 (protein KOKOPELLI-like isoform X1 [Benincasa hispida])

HSP 1 Score: 565.8 bits (1457), Expect = 4.1e-157
Identity = 357/577 (61.87%), Postives = 421/577 (72.96%), Query Frame = 0

Query: 2   IFAAWAMLSDKMDVDKLYLDLLALRELYILLLKSCLRDANSKLLDERAQILLKHLLDDAT 61
           +F   A+ S  MDVDKLYLDLLALRELYILLLKSCL DANS+LLDERAQILLKHLLDDAT
Sbjct: 20  LFRLNALCSYNMDVDKLYLDLLALRELYILLLKSCLGDANSELLDERAQILLKHLLDDAT 79

Query: 62  AEVVEFYSKILATDSSISYNFQHKDVKQTKPLDEKVAEWMEHNNQSVRKMGNLEIEHNPR 121
           A V+EF S  LAT+S+I  NF HKD KQ KPL +KV EWM+H NQ+ RKMGN EI     
Sbjct: 80  AGVLEFLSNDLATNSNIFDNFLHKDDKQVKPLADKVPEWMKH-NQTRRKMGNPEI----- 139

Query: 122 RARASASNVATNDFSNGISTALRRIEVHILSLQRCTSQSRNNTSNHIGETKLAHSGQSVL 181
           R RASASNVA N+ S+ IS+ALRRIE+HILSLQ CTSQ R          K     QSVL
Sbjct: 140 RDRASASNVAINNLSHSISSALRRIELHILSLQHCTSQRR----------KTRCHWQSVL 199

Query: 182 QRNETMNQQKVQTRTNHSTLRTGFTEPIKGHNLSSQLRSHLVGGQ-KIKPTVTNHSSEFV 241
           Q NE++NQQ V  RT  STLR+ FT+PIKG       R H VG Q K+KP   NH SE+V
Sbjct: 200 QWNESLNQQNVHPRTGPSTLRSRFTKPIKG-------RGHFVGEQKKVKPKTANHCSEYV 259

Query: 242 HGFRIPLSQDNEEAMKPPTVETYISKQQKLINPLTQIGQSGY-SVGSKVTIRAGTKLNQT 301
           HGFRIPLSQ N+EAMKP T+ET+I+KQ K++NP+T I +SGY SVGSK T R   KLNQT
Sbjct: 260 HGFRIPLSQTNDEAMKPLTIETHITKQHKVVNPMTLIDKSGYTSVGSKATFRPAMKLNQT 319

Query: 302 -RIQERRSQNSSGGMIMRPTLLD-HPSREVRKEQTYNKIHL-ATQPESEFTNSE--SESA 361
            + Q +R+QNS G M+M PTLLD HPS+E R E+  +K HL ATQ ESEFT+SE  S S+
Sbjct: 320 SKQQAKRNQNSYGQMVMGPTLLDHHPSKETRNERINSKTHLAATQQESEFTSSEFQSASS 379

Query: 362 SSSSWASQKTLESETT-----DDPSSPSYQDSPPTTGSEASTRYRSSSSSSSSISTKAFR 421
           SSSSW +Q+T  SET       +PSSPS+QD P             S+ S SS  TK F 
Sbjct: 380 SSSSWTTQETSVSETVANDGDSNPSSPSHQDDP------------LSTDSKSSSLTKTFY 439

Query: 422 FSHRKKGSKRAIGRFKRLKNKLGLIF-HHHHHHHHHHNSHNFMWK-QLRKIFHHTDNRK- 481
               K  SK+ +GRFKRLKNKLG++F HHHHHHHHHHNS+NFMWK QLRKIFH  DN++ 
Sbjct: 440 IKQGKTESKKVLGRFKRLKNKLGVVFHHHHHHHHHHHNSNNFMWKQQLRKIFHSRDNKRL 499

Query: 482 LTSNEEKSGKLKKTAIRSRGVSHKNQVGKFQALAEGLRSHVWRSKAMKKKELRGLTFGKK 541
           L S E+ + K+KK AIR+  V +KNQVGKFQALAEGLRSHVWRSKAMK+K ++G+  G K
Sbjct: 500 LVSKEDGNEKVKKRAIRN--VCYKNQVGKFQALAEGLRSHVWRSKAMKRKGVKGMKCG-K 558

Query: 542 KGVKKLHWWKMFRRRHGMKLSNKGRVRIRYVNRKSQL 564
           KGVKKLHWWKMFR R G++L NKG ++I YVN+K++L
Sbjct: 560 KGVKKLHWWKMFRNRRGVRLPNKGHMKIGYVNKKAKL 558

BLAST of Spg002279 vs. NCBI nr
Match: XP_038877123.1 (protein KOKOPELLI-like isoform X3 [Benincasa hispida] >XP_038877124.1 protein KOKOPELLI-like isoform X3 [Benincasa hispida])

HSP 1 Score: 562.8 bits (1449), Expect = 3.4e-156
Identity = 354/566 (62.54%), Postives = 416/566 (73.50%), Query Frame = 0

Query: 13  MDVDKLYLDLLALRELYILLLKSCLRDANSKLLDERAQILLKHLLDDATAEVVEFYSKIL 72
           MDVDKLYLDLLALRELYILLLKSCL DANS+LLDERAQILLKHLLDDATA V+EF S  L
Sbjct: 1   MDVDKLYLDLLALRELYILLLKSCLGDANSELLDERAQILLKHLLDDATAGVLEFLSNDL 60

Query: 73  ATDSSISYNFQHKDVKQTKPLDEKVAEWMEHNNQSVRKMGNLEIEHNPRRARASASNVAT 132
           AT+S+I  NF HKD KQ KPL +KV EWM+H NQ+ RKMGN EI     R RASASNVA 
Sbjct: 61  ATNSNIFDNFLHKDDKQVKPLADKVPEWMKH-NQTRRKMGNPEI-----RDRASASNVAI 120

Query: 133 NDFSNGISTALRRIEVHILSLQRCTSQSRNNTSNHIGETKLAHSGQSVLQRNETMNQQKV 192
           N+ S+ IS+ALRRIE+HILSLQ CTSQ R          K     QSVLQ NE++NQQ V
Sbjct: 121 NNLSHSISSALRRIELHILSLQHCTSQRR----------KTRCHWQSVLQWNESLNQQNV 180

Query: 193 QTRTNHSTLRTGFTEPIKGHNLSSQLRSHLVGGQ-KIKPTVTNHSSEFVHGFRIPLSQDN 252
             RT  STLR+ FT+PIKG       R H VG Q K+KP   NH SE+VHGFRIPLSQ N
Sbjct: 181 HPRTGPSTLRSRFTKPIKG-------RGHFVGEQKKVKPKTANHCSEYVHGFRIPLSQTN 240

Query: 253 EEAMKPPTVETYISKQQKLINPLTQIGQSGY-SVGSKVTIRAGTKLNQT-RIQERRSQNS 312
           +EAMKP T+ET+I+KQ K++NP+T I +SGY SVGSK T R   KLNQT + Q +R+QNS
Sbjct: 241 DEAMKPLTIETHITKQHKVVNPMTLIDKSGYTSVGSKATFRPAMKLNQTSKQQAKRNQNS 300

Query: 313 SGGMIMRPTLLD-HPSREVRKEQTYNKIHL-ATQPESEFTNSE--SESASSSSWASQKTL 372
            G M+M PTLLD HPS+E R E+  +K HL ATQ ESEFT+SE  S S+SSSSW +Q+T 
Sbjct: 301 YGQMVMGPTLLDHHPSKETRNERINSKTHLAATQQESEFTSSEFQSASSSSSSWTTQETS 360

Query: 373 ESETT-----DDPSSPSYQDSPPTTGSEASTRYRSSSSSSSSISTKAFRFSHRKKGSKRA 432
            SET       +PSSPS+QD P             S+ S SS  TK F     K  SK+ 
Sbjct: 361 VSETVANDGDSNPSSPSHQDDP------------LSTDSKSSSLTKTFYIKQGKTESKKV 420

Query: 433 IGRFKRLKNKLGLIF-HHHHHHHHHHNSHNFMWK-QLRKIFHHTDNRK-LTSNEEKSGKL 492
           +GRFKRLKNKLG++F HHHHHHHHHHNS+NFMWK QLRKIFH  DN++ L S E+ + K+
Sbjct: 421 LGRFKRLKNKLGVVFHHHHHHHHHHHNSNNFMWKQQLRKIFHSRDNKRLLVSKEDGNEKV 480

Query: 493 KKTAIRSRGVSHKNQVGKFQALAEGLRSHVWRSKAMKKKELRGLTFGKKKGVKKLHWWKM 552
           KK AIR+  V +KNQVGKFQALAEGLRSHVWRSKAMK+K ++G+  G KKGVKKLHWWKM
Sbjct: 481 KKRAIRN--VCYKNQVGKFQALAEGLRSHVWRSKAMKRKGVKGMKCG-KKGVKKLHWWKM 528

Query: 553 FRRRHGMKLSNKGRVRIRYVNRKSQL 564
           FR R G++L NKG ++I YVN+K++L
Sbjct: 541 FRNRRGVRLPNKGHMKIGYVNKKAKL 528

BLAST of Spg002279 vs. NCBI nr
Match: KAG6579634.1 (Protein KOKOPELLI, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 552.7 bits (1423), Expect = 3.6e-153
Identity = 334/552 (60.51%), Postives = 400/552 (72.46%), Query Frame = 0

Query: 13  MDVDKLYLDLLALRELYILLLKSCLRDANSKLLDERAQILLKHLLDDATAEVVEFYSKIL 72
           MDVD+ YLDLLALRELYILLLKSCLRDA S+LLD RAQILLK+LLDDATAEV+EF  K +
Sbjct: 1   MDVDESYLDLLALRELYILLLKSCLRDAPSELLDGRAQILLKNLLDDATAEVLEFLPKNM 60

Query: 73  ATDSSISYNFQHKDVKQTKPLDEKVAEWMEHNNQSVRKMGNLEIEHNPRRARASASNVAT 132
           ATDS I Y F HKD KQ+KPLDEKV EWM               +H P+RAR SASN  T
Sbjct: 61  ATDSGIFYKFLHKDDKQSKPLDEKVVEWM---------------KHIPKRARGSASNATT 120

Query: 133 NDFSNGISTALRRIEVHILSLQRCTSQSRNNTSNHIGETKLAHSGQSVLQRNETMNQQKV 192
           +    GIS+ALRRIE HILSLQR TSQS+         + +++ G+SVL+ NET+N+QKV
Sbjct: 121 DLILQGISSALRRIEHHILSLQRYTSQSK--------RSHISYCGRSVLKGNETLNRQKV 180

Query: 193 QTRTNHSTLRTGFTEPIKGHNLSSQLRSHLVGGQKIKPTVTNHSSEFVHGFRIPLSQDNE 252
           Q+RT+HST+             + Q++ HLVGGQ +K  VT H SEFVHGFR+PLSQ +E
Sbjct: 181 QSRTDHSTIS------------ARQIKGHLVGGQNVKAVVTPHRSEFVHGFRLPLSQGSE 240

Query: 253 EAMKPPTVETYISKQQKLINPLTQIGQSGYSVGSKVTIRAGTKLNQTRIQERRSQNSSGG 312
           E  KP TVET++SKQ KL+NP+T I +SG SVGSK TIR   K +Q+R+  ++SQNS G 
Sbjct: 241 EGRKPLTVETHLSKQHKLVNPMTPIDKSGGSVGSKATIRPRKKPSQSRV--KKSQNSYGL 300

Query: 313 MIMRPTLLDHPSREVRKEQTYNKIHLATQPESEFTNSESESASSSSWASQKTLESETTDD 372
           M+M+PTLLDHPSREVRKE+T  K HLATQ ESEFT    +SA SSSW +Q+T ESET DD
Sbjct: 301 MVMKPTLLDHPSREVRKEETQKKTHLATQHESEFT----DSACSSSWTTQQTSESETLDD 360

Query: 373 PSSPSYQDSPPTTGSEASTRYRSSSSSSSSISTKAFRFSHRKKGSKRAIGRFKRLKNKLG 432
            SSPS+QD  P   SEAS+                 R+SH KK SKRAIGRFKRLKNKLG
Sbjct: 361 FSSPSHQDERPANSSEASSS----------------RYSHGKKESKRAIGRFKRLKNKLG 420

Query: 433 LIFHHHHHHHHHHNSHNFMWKQLRKIFHHTDNRKLTSNEEKSGKLKKTAIRSRGVSHKNQ 492
           +IF HHHHHHHHHNSH+FMW ++RKIFH T+N+KLTS E++  K K TAIRS      NQ
Sbjct: 421 IIF-HHHHHHHHHNSHSFMWNRVRKIFHPTNNKKLTSMEDRYEKGKNTAIRSE--CRTNQ 480

Query: 493 VGKFQALAEGLRSHVWRSKAMKKKELRGLTFGKKKGVKKLHWWKMFRRRHGMKLSNKGRV 552
           VGKFQA+A+ L+SHV RSK +KKK+   +  G  KGVKKLHWWK+FR RHG++  NKGR+
Sbjct: 481 VGKFQAIAKELQSHVRRSKELKKKDPWEMKCG--KGVKKLHWWKLFRNRHGVRFHNKGRI 490

Query: 553 -RIRYVNRKSQL 564
            RIRYVN+K QL
Sbjct: 541 RRIRYVNKKPQL 490

BLAST of Spg002279 vs. NCBI nr
Match: KAG7017089.1 (Protein KOKOPELLI, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 552.7 bits (1423), Expect = 3.6e-153
Identity = 334/552 (60.51%), Postives = 400/552 (72.46%), Query Frame = 0

Query: 13  MDVDKLYLDLLALRELYILLLKSCLRDANSKLLDERAQILLKHLLDDATAEVVEFYSKIL 72
           MDVD+ YLDLLALRELYILLLKSCLRDA S+LLD RAQILLK+LLDDATAEV+EF  K +
Sbjct: 75  MDVDESYLDLLALRELYILLLKSCLRDAPSELLDGRAQILLKNLLDDATAEVLEFLPKNM 134

Query: 73  ATDSSISYNFQHKDVKQTKPLDEKVAEWMEHNNQSVRKMGNLEIEHNPRRARASASNVAT 132
           ATDS I Y F HKD KQ+KPLDEKV EWM               +H P+RAR SASN  T
Sbjct: 135 ATDSGIFYKFLHKDDKQSKPLDEKVVEWM---------------KHIPKRARGSASNATT 194

Query: 133 NDFSNGISTALRRIEVHILSLQRCTSQSRNNTSNHIGETKLAHSGQSVLQRNETMNQQKV 192
           +    GIS+ALRRIE HILSLQR TSQS+         + +++ G+SVL+ NET+N+QKV
Sbjct: 195 DLILQGISSALRRIEHHILSLQRYTSQSK--------RSHISYCGRSVLKGNETLNRQKV 254

Query: 193 QTRTNHSTLRTGFTEPIKGHNLSSQLRSHLVGGQKIKPTVTNHSSEFVHGFRIPLSQDNE 252
           Q+RT+HST+             + Q++ HLVGGQ +K  VT H SEFVHGFR+PLSQ +E
Sbjct: 255 QSRTDHSTIS------------ARQIKGHLVGGQNVKAVVTPHRSEFVHGFRLPLSQGSE 314

Query: 253 EAMKPPTVETYISKQQKLINPLTQIGQSGYSVGSKVTIRAGTKLNQTRIQERRSQNSSGG 312
           E  KP TVET++SKQ KL+NP+T I +SG SVGSK TIR   K +Q+R+  ++SQNS G 
Sbjct: 315 EGRKPLTVETHLSKQHKLVNPMTPIDKSGGSVGSKATIRPRKKPSQSRV--KKSQNSYGL 374

Query: 313 MIMRPTLLDHPSREVRKEQTYNKIHLATQPESEFTNSESESASSSSWASQKTLESETTDD 372
           M+M+PTLLDHPSREVRKE+T  K HLATQ ESEFT    +SA SSSW +Q+T ESET DD
Sbjct: 375 MVMKPTLLDHPSREVRKEETQKKTHLATQHESEFT----DSACSSSWTTQQTSESETLDD 434

Query: 373 PSSPSYQDSPPTTGSEASTRYRSSSSSSSSISTKAFRFSHRKKGSKRAIGRFKRLKNKLG 432
            SSPS+QD  P   SEAS+                 R+SH KK SKRAIGRFKRLKNKLG
Sbjct: 435 FSSPSHQDERPANSSEASSS----------------RYSHGKKESKRAIGRFKRLKNKLG 494

Query: 433 LIFHHHHHHHHHHNSHNFMWKQLRKIFHHTDNRKLTSNEEKSGKLKKTAIRSRGVSHKNQ 492
           +IF HHHHHHHHHNSH+FMW ++RKIFH T+N+KLTS E++  K K TAIRS      NQ
Sbjct: 495 IIF-HHHHHHHHHNSHSFMWNRVRKIFHPTNNKKLTSMEDRYEKGKNTAIRSE--CRTNQ 554

Query: 493 VGKFQALAEGLRSHVWRSKAMKKKELRGLTFGKKKGVKKLHWWKMFRRRHGMKLSNKGRV 552
           VGKFQA+A+ L+SHV RSK +KKK+   +  G  KGVKKLHWWK+FR RHG++  NKGR+
Sbjct: 555 VGKFQAIAKELQSHVRRSKELKKKDPWEMKCG--KGVKKLHWWKLFRNRHGVRFHNKGRI 564

Query: 553 -RIRYVNRKSQL 564
            RIRYVN+K QL
Sbjct: 615 RRIRYVNKKPQL 564

BLAST of Spg002279 vs. NCBI nr
Match: XP_022996025.1 (uncharacterized protein LOC111491355 isoform X1 [Cucurbita maxima] >XP_022996026.1 uncharacterized protein LOC111491355 isoform X1 [Cucurbita maxima])

HSP 1 Score: 545.0 bits (1403), Expect = 7.4e-151
Identity = 341/565 (60.35%), Postives = 398/565 (70.44%), Query Frame = 0

Query: 9   LSDKMDVDKLYLDLLALRELYILLLKSCLRDANSKL-LDERAQILLKHLLDDATAEVVEF 68
           LSDKM+ D+LYLDLLALR+LY  LLK CLRDANS+L +  RA+ILLKHLLDDAT  ++EF
Sbjct: 41  LSDKMEADELYLDLLALRQLYFFLLKCCLRDANSELVVGARAKILLKHLLDDATTGLLEF 100

Query: 69  YSKILATDSSISYNFQHKDVKQTKPLDEKVAEWMEHNNQSVRKMGNLE-IEHNPRRARAS 128
           +SK LA      YNF  KD KQTKPLDEKVAEWMEH NQ+ R+M N E IEH PRR RAS
Sbjct: 101 HSKTLA-----FYNFLRKDDKQTKPLDEKVAEWMEH-NQTARRMANPEKIEHKPRRDRAS 160

Query: 129 ASNVATNDFSNGISTALRRIEVHILSLQRCTSQSRNNTSNHIGETKLAHSGQSVLQRNET 188
           ASNVA ND S+GI++ALRRIE+HILSLQR        T +HI ETKLA+ GQSV Q NE+
Sbjct: 161 ASNVAANDLSSGINSALRRIELHILSLQR-------YTRSHISETKLAYYGQSVNQGNES 220

Query: 189 MNQQKVQTRTNHSTLRTGFTEPIKGHNLSSQLRSHLVGGQKIKPTVTNHSSEFVHGFRIP 248
            NQQKV                                    KP V NH S+FV+GFRIP
Sbjct: 221 FNQQKV------------------------------------KPMVANHCSKFVNGFRIP 280

Query: 249 LSQDNEEAMKPPTVETYISKQQKLINPLTQIGQSGYSVGSKVTIRAGTKLNQTRIQERRS 308
           L+QD +EAM          KQ +L+ P T + +SG   GSK T R   KLN+T IQE+RS
Sbjct: 281 LTQDKDEAM----------KQHELVLPPTLMDKSGCPEGSKATARRAMKLNRTWIQEKRS 340

Query: 309 QNSSGGMIMRPTLLDHPSREVRKEQT-YNKIHLATQPESEFTNSESESASSSSWASQKTL 368
           +NS G ++M+PTL  HPSREVRKEQT +N+ HLA Q ESEFTN  SESAS SS A+ +T 
Sbjct: 341 KNSRGRIVMKPTLWHHPSREVRKEQTHHNRRHLAAQQESEFTN--SESASCSSPATLQTS 400

Query: 369 ESETTDDPSSPSYQDSPPTTGSEASTRYRSSSSSSSSISTKAFRFSHRKKGSKRAIGRFK 428
           ESETTDD SSP  Q SP  TGSEAS++Y    +SSS+I+ KAF+FSH KK S  A+GRFK
Sbjct: 401 ESETTDDSSSPDNQSSPTATGSEASSQY---GNSSSNITRKAFKFSHGKKESNGAVGRFK 460

Query: 429 RLKNKLGLIFHHH----HHHHHHHNSHNFMWKQLRKIFHHTDNRKLTSNEEKSGKLKKTA 488
            L+NKLGLIFHHH    HHHHHHH+ HN MWKQ+R +FH TD ++LTS EEK+GKL+KT 
Sbjct: 461 SLRNKLGLIFHHHQHHQHHHHHHHHGHNSMWKQVRTVFHRTDKKELTSKEEKTGKLRKTT 520

Query: 489 IRSRGVSHKNQVGKFQALAEGLRSHVWRSKAMKKKELRGLTFGKKKGVKKLHWWKMFRRR 548
           IRS  VS  NQVGKFQAL EGLRSHVW+SKAMKKKE RGL  G     KKLHWWKM RRR
Sbjct: 521 IRS--VSRNNQVGKFQALPEGLRSHVWKSKAMKKKEQRGLNCG-----KKLHWWKMIRRR 534

Query: 549 HGMKLSNKGRVRIRYVNRKSQLKVV 567
            G+K  NKGRV+I YVNRK  +K++
Sbjct: 581 RGVKFPNKGRVKIGYVNRKPDVKLI 534

BLAST of Spg002279 vs. ExPASy Swiss-Prot
Match: Q9FFP2 (Protein KOKOPELLI OS=Arabidopsis thaliana OX=3702 GN=KPL PE=2 SV=1)

HSP 1 Score: 87.8 bits (216), Expect = 4.2e-16
Identity = 88/263 (33.46%), Postives = 131/263 (49.81%), Query Frame = 0

Query: 314 IMRPTLLDH-------PSREVRKEQTYNKIHLATQPE----SEFTNSESESASSSSWASQ 373
           IM+PTL+D         S E   +QT +     ++ E    S+  + E+ S+S S W +Q
Sbjct: 248 IMKPTLMDQETETFDDDSSETEADQTPSATGSESEDEEVSTSQEYSGETGSSSGSEWETQ 307

Query: 374 KTLESETTDDPSSPSYQDSPPTTGSEASTRYRSSSSSSSSISTKAFRFSHRKKGSKR--A 433
              ++E+  + S P   D               S S  S+      R + R+ G +R   
Sbjct: 308 AENDTESKSESSYPPQND--------------DSVSEVSTSPPHTDRDTSREPGKQRRNV 367

Query: 434 IGRFKRLKNKLGLIFHHHHHHHHHHNSHN----FMWKQLRKIFHHTDNRKLTSNEEKSGK 493
           +GRFKR+KNK+G IFHHHHHHHHHH+ H+      W +L+  FHH        ++EKS +
Sbjct: 368 MGRFKRIKNKIGQIFHHHHHHHHHHHHHDKEKPSAWNKLQSKFHH-------KHQEKSKE 427

Query: 494 LKKTAIRSRGV-SHK--NQVGKFQALAEGLRSHVWRSKAMKKKELRGLTFGKKKGVKKLH 553
            K+    S+G+ +HK  +Q G F AL EGL  H   SK  K +         K   KK  
Sbjct: 428 RKRPMSESKGLTTHKQQHQGGHFHALVEGLVRHRKHSKKQKHQ--------LKSDAKKTE 481

Query: 554 WWKMFRRRH--GMKLSNKGRVRI 555
           WWK+ ++R   G+K+  +GRV++
Sbjct: 488 WWKLLKKRQGGGVKIPKRGRVKL 481

BLAST of Spg002279 vs. ExPASy TrEMBL
Match: A0A6J1K5J4 (uncharacterized protein LOC111491355 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111491355 PE=4 SV=1)

HSP 1 Score: 545.0 bits (1403), Expect = 3.6e-151
Identity = 341/565 (60.35%), Postives = 398/565 (70.44%), Query Frame = 0

Query: 9   LSDKMDVDKLYLDLLALRELYILLLKSCLRDANSKL-LDERAQILLKHLLDDATAEVVEF 68
           LSDKM+ D+LYLDLLALR+LY  LLK CLRDANS+L +  RA+ILLKHLLDDAT  ++EF
Sbjct: 41  LSDKMEADELYLDLLALRQLYFFLLKCCLRDANSELVVGARAKILLKHLLDDATTGLLEF 100

Query: 69  YSKILATDSSISYNFQHKDVKQTKPLDEKVAEWMEHNNQSVRKMGNLE-IEHNPRRARAS 128
           +SK LA      YNF  KD KQTKPLDEKVAEWMEH NQ+ R+M N E IEH PRR RAS
Sbjct: 101 HSKTLA-----FYNFLRKDDKQTKPLDEKVAEWMEH-NQTARRMANPEKIEHKPRRDRAS 160

Query: 129 ASNVATNDFSNGISTALRRIEVHILSLQRCTSQSRNNTSNHIGETKLAHSGQSVLQRNET 188
           ASNVA ND S+GI++ALRRIE+HILSLQR        T +HI ETKLA+ GQSV Q NE+
Sbjct: 161 ASNVAANDLSSGINSALRRIELHILSLQR-------YTRSHISETKLAYYGQSVNQGNES 220

Query: 189 MNQQKVQTRTNHSTLRTGFTEPIKGHNLSSQLRSHLVGGQKIKPTVTNHSSEFVHGFRIP 248
            NQQKV                                    KP V NH S+FV+GFRIP
Sbjct: 221 FNQQKV------------------------------------KPMVANHCSKFVNGFRIP 280

Query: 249 LSQDNEEAMKPPTVETYISKQQKLINPLTQIGQSGYSVGSKVTIRAGTKLNQTRIQERRS 308
           L+QD +EAM          KQ +L+ P T + +SG   GSK T R   KLN+T IQE+RS
Sbjct: 281 LTQDKDEAM----------KQHELVLPPTLMDKSGCPEGSKATARRAMKLNRTWIQEKRS 340

Query: 309 QNSSGGMIMRPTLLDHPSREVRKEQT-YNKIHLATQPESEFTNSESESASSSSWASQKTL 368
           +NS G ++M+PTL  HPSREVRKEQT +N+ HLA Q ESEFTN  SESAS SS A+ +T 
Sbjct: 341 KNSRGRIVMKPTLWHHPSREVRKEQTHHNRRHLAAQQESEFTN--SESASCSSPATLQTS 400

Query: 369 ESETTDDPSSPSYQDSPPTTGSEASTRYRSSSSSSSSISTKAFRFSHRKKGSKRAIGRFK 428
           ESETTDD SSP  Q SP  TGSEAS++Y    +SSS+I+ KAF+FSH KK S  A+GRFK
Sbjct: 401 ESETTDDSSSPDNQSSPTATGSEASSQY---GNSSSNITRKAFKFSHGKKESNGAVGRFK 460

Query: 429 RLKNKLGLIFHHH----HHHHHHHNSHNFMWKQLRKIFHHTDNRKLTSNEEKSGKLKKTA 488
            L+NKLGLIFHHH    HHHHHHH+ HN MWKQ+R +FH TD ++LTS EEK+GKL+KT 
Sbjct: 461 SLRNKLGLIFHHHQHHQHHHHHHHHGHNSMWKQVRTVFHRTDKKELTSKEEKTGKLRKTT 520

Query: 489 IRSRGVSHKNQVGKFQALAEGLRSHVWRSKAMKKKELRGLTFGKKKGVKKLHWWKMFRRR 548
           IRS  VS  NQVGKFQAL EGLRSHVW+SKAMKKKE RGL  G     KKLHWWKM RRR
Sbjct: 521 IRS--VSRNNQVGKFQALPEGLRSHVWKSKAMKKKEQRGLNCG-----KKLHWWKMIRRR 534

Query: 549 HGMKLSNKGRVRIRYVNRKSQLKVV 567
            G+K  NKGRV+I YVNRK  +K++
Sbjct: 581 RGVKFPNKGRVKIGYVNRKPDVKLI 534

BLAST of Spg002279 vs. ExPASy TrEMBL
Match: A0A6J1K0S1 (uncharacterized protein LOC111491355 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111491355 PE=4 SV=1)

HSP 1 Score: 537.3 bits (1383), Expect = 7.5e-149
Identity = 337/561 (60.07%), Postives = 394/561 (70.23%), Query Frame = 0

Query: 13  MDVDKLYLDLLALRELYILLLKSCLRDANSKL-LDERAQILLKHLLDDATAEVVEFYSKI 72
           M+ D+LYLDLLALR+LY  LLK CLRDANS+L +  RA+ILLKHLLDDAT  ++EF+SK 
Sbjct: 1   MEADELYLDLLALRQLYFFLLKCCLRDANSELVVGARAKILLKHLLDDATTGLLEFHSKT 60

Query: 73  LATDSSISYNFQHKDVKQTKPLDEKVAEWMEHNNQSVRKMGNLE-IEHNPRRARASASNV 132
           LA      YNF  KD KQTKPLDEKVAEWMEH NQ+ R+M N E IEH PRR RASASNV
Sbjct: 61  LA-----FYNFLRKDDKQTKPLDEKVAEWMEH-NQTARRMANPEKIEHKPRRDRASASNV 120

Query: 133 ATNDFSNGISTALRRIEVHILSLQRCTSQSRNNTSNHIGETKLAHSGQSVLQRNETMNQQ 192
           A ND S+GI++ALRRIE+HILSLQR        T +HI ETKLA+ GQSV Q NE+ NQQ
Sbjct: 121 AANDLSSGINSALRRIELHILSLQR-------YTRSHISETKLAYYGQSVNQGNESFNQQ 180

Query: 193 KVQTRTNHSTLRTGFTEPIKGHNLSSQLRSHLVGGQKIKPTVTNHSSEFVHGFRIPLSQD 252
           KV                                    KP V NH S+FV+GFRIPL+QD
Sbjct: 181 KV------------------------------------KPMVANHCSKFVNGFRIPLTQD 240

Query: 253 NEEAMKPPTVETYISKQQKLINPLTQIGQSGYSVGSKVTIRAGTKLNQTRIQERRSQNSS 312
            +EAM          KQ +L+ P T + +SG   GSK T R   KLN+T IQE+RS+NS 
Sbjct: 241 KDEAM----------KQHELVLPPTLMDKSGCPEGSKATARRAMKLNRTWIQEKRSKNSR 300

Query: 313 GGMIMRPTLLDHPSREVRKEQT-YNKIHLATQPESEFTNSESESASSSSWASQKTLESET 372
           G ++M+PTL  HPSREVRKEQT +N+ HLA Q ESEFTN  SESAS SS A+ +T ESET
Sbjct: 301 GRIVMKPTLWHHPSREVRKEQTHHNRRHLAAQQESEFTN--SESASCSSPATLQTSESET 360

Query: 373 TDDPSSPSYQDSPPTTGSEASTRYRSSSSSSSSISTKAFRFSHRKKGSKRAIGRFKRLKN 432
           TDD SSP  Q SP  TGSEAS++Y    +SSS+I+ KAF+FSH KK S  A+GRFK L+N
Sbjct: 361 TDDSSSPDNQSSPTATGSEASSQY---GNSSSNITRKAFKFSHGKKESNGAVGRFKSLRN 420

Query: 433 KLGLIFHHH----HHHHHHHNSHNFMWKQLRKIFHHTDNRKLTSNEEKSGKLKKTAIRSR 492
           KLGLIFHHH    HHHHHHH+ HN MWKQ+R +FH TD ++LTS EEK+GKL+KT IRS 
Sbjct: 421 KLGLIFHHHQHHQHHHHHHHHGHNSMWKQVRTVFHRTDKKELTSKEEKTGKLRKTTIRS- 480

Query: 493 GVSHKNQVGKFQALAEGLRSHVWRSKAMKKKELRGLTFGKKKGVKKLHWWKMFRRRHGMK 552
            VS  NQVGKFQAL EGLRSHVW+SKAMKKKE RGL  G     KKLHWWKM RRR G+K
Sbjct: 481 -VSRNNQVGKFQALPEGLRSHVWKSKAMKKKEQRGLNCG-----KKLHWWKMIRRRRGVK 490

Query: 553 LSNKGRVRIRYVNRKSQLKVV 567
             NKGRV+I YVNRK  +K++
Sbjct: 541 FPNKGRVKIGYVNRKPDVKLI 490

BLAST of Spg002279 vs. ExPASy TrEMBL
Match: A0A6J1I0S9 (protein KOKOPELLI-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111468843 PE=4 SV=1)

HSP 1 Score: 536.2 bits (1380), Expect = 1.7e-148
Identity = 326/553 (58.95%), Postives = 399/553 (72.15%), Query Frame = 0

Query: 13  MDVDKLYLDLLALRELYILLLKSCLRDANSKLLDERAQILLKHLLDDATAEVVEFYSKIL 72
           MDVD+ YLDLLALRELYILLLKSCLRDA S+LLDERAQILLK+ LDDATAEV+EF SK L
Sbjct: 18  MDVDESYLDLLALRELYILLLKSCLRDAPSELLDERAQILLKNFLDDATAEVLEFLSKNL 77

Query: 73  ATDSSISYNFQHKDVKQTKPLDEKVAEWMEHNNQSVRKMGNLEIEHNPRRARASASNVAT 132
           ATDS I Y F HKD KQTKPLDEKV E M               +H P+RAR SAS   T
Sbjct: 78  ATDSGIFYKFLHKDNKQTKPLDEKVVERM---------------KHIPKRARGSASKATT 137

Query: 133 NDFSNGISTALRRIEVHILSLQRCTSQSRNNTSNHIGETKLAHSGQSVLQRNETMNQQKV 192
           +    GI +ALRRIE HILS QR  SQS+         + +++ G+SVL+ NET+N+QKV
Sbjct: 138 DLILQGIRSALRRIEHHILSRQRYISQSK--------RSHISYCGRSVLKGNETLNRQKV 197

Query: 193 QTRTNHSTLRTGFTEPIKGHNLSSQLRSHLVGGQKIKPTVTNHSSEFVHGFRIPLSQDNE 252
           Q+RT+HST+             + Q++ HLVGGQ +KP ++ H SEFVHGFR+PLSQ N 
Sbjct: 198 QSRTDHSTIS------------ARQIKGHLVGGQNVKPVLSPHCSEFVHGFRLPLSQGNA 257

Query: 253 EAMKPPTVETYISKQQKLINPLTQIGQSGYSVGSKVTIRAGTKLNQTRIQERRSQNSSG- 312
           E  KP  VET++SKQ K +NP+T+I +SG SVGSK TI    K +Q+R+  +RS+NS G 
Sbjct: 258 EGRKPLAVETHLSKQHKFVNPMTRIDRSGGSVGSKATIMPRKKPSQSRV--KRSKNSYGP 317

Query: 313 GMIMRPTLLDHPSREVRKEQTYNKIHLATQPESEFTNSESESASSSSWASQKTLESETTD 372
            M+M+PTLL+HPSREVRKE+T NK HLA+Q E+EFT    +SASSSSW +Q+T ESET D
Sbjct: 318 HMVMKPTLLEHPSREVRKEETQNKTHLASQQEAEFT----DSASSSSWTTQQTSESETLD 377

Query: 373 DPSSPSYQDSPPTTGSEASTRYRSSSSSSSSISTKAFRFSHRKKGSKRAIGRFKRLKNKL 432
           + SSPS+QD PP   S+AS+R                R+SH KK SKRAIGRFKRLKNKL
Sbjct: 378 EFSSPSHQDEPPANSSKASSR----------------RYSHGKKESKRAIGRFKRLKNKL 437

Query: 433 GLIFHHHHHHHHHHNSHNFMWKQLRKIFHHTDNRKLTSNEEKSGKLKKTAIRSRGVSHKN 492
           G+IF HHHHHHHHHN H+FMW ++RKIFH T+N+KLTS E++  K+K TA+RS G +  N
Sbjct: 438 GIIF-HHHHHHHHHNRHSFMWNRVRKIFHPTNNKKLTSMEDRYEKVKNTAVRSEGWT--N 497

Query: 493 QVGKFQALAEGLRSHVWRSKAMKKKELRGLTFGKKKGVKKLHWWKMFRRRHGMKLSNKGR 552
           QV KFQA+A+ L+SHV RSKAMKKK+   +  G  KGVKKLHWWK+F  RHG++  NKG 
Sbjct: 498 QVSKFQAIAKELQSHVRRSKAMKKKDPWKMKCG--KGVKKLHWWKLFCNRHGVRFHNKGC 508

Query: 553 V-RIRYVNRKSQL 564
           + RIRYVNRKS+L
Sbjct: 558 IRRIRYVNRKSKL 508

BLAST of Spg002279 vs. ExPASy TrEMBL
Match: A0A6J1ETH9 (protein KOKOPELLI-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111435826 PE=4 SV=1)

HSP 1 Score: 535.8 bits (1379), Expect = 2.2e-148
Identity = 329/552 (59.60%), Postives = 394/552 (71.38%), Query Frame = 0

Query: 13  MDVDKLYLDLLALRELYILLLKSCLRDANSKLLDERAQILLKHLLDDATAEVVEFYSKIL 72
           MDVD+ YLDLLALRELYILLLKSCLRDA S+LLDERAQILLK+LLDDATAEV+EF  K +
Sbjct: 1   MDVDESYLDLLALRELYILLLKSCLRDAPSELLDERAQILLKNLLDDATAEVLEFLPKNM 60

Query: 73  ATDSSISYNFQHKDVKQTKPLDEKVAEWMEHNNQSVRKMGNLEIEHNPRRARASASNVAT 132
           ATDS I Y F HKD KQ+KPLDEKV EWM               +  P+RAR SASN  T
Sbjct: 61  ATDSGIFYKFLHKDDKQSKPLDEKVVEWM---------------KPIPKRARGSASNATT 120

Query: 133 NDFSNGISTALRRIEVHILSLQRCTSQSRNNTSNHIGETKLAHSGQSVLQRNETMNQQKV 192
           +    GIS+A+RRIE HILSLQR TSQS+         + +++ G+SVL+ NET N+QKV
Sbjct: 121 DLILQGISSAIRRIEHHILSLQRYTSQSK--------RSHISYCGRSVLKGNETSNRQKV 180

Query: 193 QTRTNHSTLRTGFTEPIKGHNLSSQLRSHLVGGQKIKPTVTNHSSEFVHGFRIPLSQDNE 252
           Q+RT+HST+             + Q++  LVGGQ  K  VT H SEFVHGFR+PLSQ ++
Sbjct: 181 QSRTDHSTIS------------ARQIKGLLVGGQNAKAVVTPHCSEFVHGFRLPLSQGSK 240

Query: 253 EAMKPPTVETYISKQQKLINPLTQIGQSGYSVGSKVTIRAGTKLNQTRIQERRSQNSSGG 312
           E  KP  VET++SKQ KL+NP+T I + G SVGSK TIR   K +Q+R+  ++SQNS G 
Sbjct: 241 EGRKPLAVETHLSKQHKLVNPMTLIDKCGGSVGSKATIRPRKKPSQSRV--KKSQNSYGL 300

Query: 313 MIMRPTLLDHPSREVRKEQTYNKIHLATQPESEFTNSESESASSSSWASQKTLESETTDD 372
           M+M+PTLLDHPSREVRKE+T  K HLATQ ESEFT    +SA SSSW +Q+T ES T DD
Sbjct: 301 MVMKPTLLDHPSREVRKEETQKKTHLATQHESEFT----DSACSSSWTTQQTSESGTLDD 360

Query: 373 PSSPSYQDSPPTTGSEASTRYRSSSSSSSSISTKAFRFSHRKKGSKRAIGRFKRLKNKLG 432
            SSPS+QD  P   SE                T + R+S  KK SKRAIGRFKRLKNKLG
Sbjct: 361 FSSPSHQDERPANSSE----------------TSSIRYSQGKKESKRAIGRFKRLKNKLG 420

Query: 433 LIFHHHHHHHHHHNSHNFMWKQLRKIFHHTDNRKLTSNEEKSGKLKKTAIRSRGVSHKNQ 492
           +IF HHHHHHHHHNSH+FMW ++RKIFH T+N+KLTS E++  K K TAIRS      NQ
Sbjct: 421 IIF-HHHHHHHHHNSHSFMWNRVRKIFHPTNNKKLTSMEDRYEKGKNTAIRSE--CRTNQ 480

Query: 493 VGKFQALAEGLRSHVWRSKAMKKKELRGLTFGKKKGVKKLHWWKMFRRRHGMKLSNKGRV 552
           VGKFQA+A+ LRSHV RSKA+ KK+   +  G KKGVKKLHWWK+FR RHG++L NKGR+
Sbjct: 481 VGKFQAIAKELRSHVRRSKALTKKDPWEMKCG-KKGVKKLHWWKLFRDRHGVRLHNKGRI 491

Query: 553 -RIRYVNRKSQL 564
            RIRYVN+K QL
Sbjct: 541 RRIRYVNKKPQL 491

BLAST of Spg002279 vs. ExPASy TrEMBL
Match: A0A6J1DNR3 (protein KOKOPELLI isoform X2 OS=Momordica charantia OX=3673 GN=LOC111022084 PE=4 SV=1)

HSP 1 Score: 534.3 bits (1375), Expect = 6.3e-148
Identity = 339/558 (60.75%), Postives = 388/558 (69.53%), Query Frame = 0

Query: 13  MDVDKLYLDLLALRELYILLLKSCLRDANSKLLDERAQILLKHLLDDATAEVVEFYSKIL 72
           M+V++LYLDLLALRELYILLLKSCLRDANS+LLDERAQILLKHLLDDATAE+V+F+SK  
Sbjct: 1   MEVNELYLDLLALRELYILLLKSCLRDANSELLDERAQILLKHLLDDATAEIVQFHSK-- 60

Query: 73  ATDSSISYNFQHKDVKQTKPLDEKVAEWMEHNNQSVRKMGNLEIEHNPRRARASASNVAT 132
                            TKP++EKVAEWME+ NQS RK G                NVA 
Sbjct: 61  -----------------TKPVEEKVAEWMEY-NQSTRKTG----------------NVAA 120

Query: 133 NDFSNGISTALRRIEVHILSLQRCTSQSRNNTSNHIGETKLAHSGQSVLQRNETMNQQKV 192
           ND SNGI  ALRRIE HILSLQ  TSQSR NT +HI   KL+         N  ++QQKV
Sbjct: 121 NDLSNGIGLALRRIEFHILSLQHYTSQSR-NTRSHINGAKLS---------NSPLDQQKV 180

Query: 193 QTRTNHSTLRTGFTEPIKGHNLSSQLRSHLVGGQKIKPTVTNHSSEFVHGFRIPLSQDNE 252
           Q+R +HS L+    EPI G                       H SEFVHGFR+PLSQDN 
Sbjct: 181 QSRMDHSNLKARVAEPING-----------------------HCSEFVHGFRVPLSQDNV 240

Query: 253 EAMKPPTVETYISKQQKLINPLTQIGQSGYSVGSKVTIRAGTKLNQTRIQERRSQNSSGG 312
           EAMKPP V T +SKQ K+INP+  I +S  SVGSK T+R+   +N+T+I ERR QN  G 
Sbjct: 241 EAMKPPNVGTQVSKQNKVINPVILIDKSRCSVGSKATVRS---VNRTQIHERRCQNLPGH 300

Query: 313 MIMRPTLLDHPSREVRKEQTYNKIHLATQPESEFTNSESESASSSSWASQKTLESETTDD 372
           MIMRPTLL+H            K  + TQ ESEFTNSESES SSSSWA+Q+T E+ETTD 
Sbjct: 301 MIMRPTLLNH-----------MKTRMPTQQESEFTNSESESVSSSSWATQQTSETETTDY 360

Query: 373 PSSPSYQDSPPTTGSEASTRYRSSSSSSSSISTKAFRFSHRKKGSKRAIGRFKRLKNKLG 432
           PSS S+Q+  P TGSE S+RYR     SS IS+KAFR SH KKGSK+AIGRFKRL+NKLG
Sbjct: 361 PSSSSHQEDQPATGSEVSSRYR-----SSRISSKAFRISHGKKGSKKAIGRFKRLRNKLG 420

Query: 433 LIF--HHHHHHHHHHNSHN--FMWKQLRKIFHHTDNRKLTSNEEKSGKLKKTAIRSRGVS 492
           LIF  HHHHHHHHHHNSHN  FMWKQLRKIFH TD +++TS + +   LKKTAIRS  VS
Sbjct: 421 LIFHHHHHHHHHHHHNSHNNFFMWKQLRKIFHGTDKKRVTS-KGRHETLKKTAIRS--VS 466

Query: 493 HKNQVGKFQALAEGLRSHVWRSKAMKKKELRGLTFGKKKGVKKLHWWKMFRRRHGMKLSN 552
            KNQVG+FQALAEGLRSHVW+  AMKKKELR    G KKGVKKLHWW+MF RR G+KL N
Sbjct: 481 RKNQVGRFQALAEGLRSHVWKPTAMKKKELRKPRLG-KKGVKKLHWWRMFCRRRGVKLPN 466

Query: 553 KGRVRIRYVNRKSQLKVV 567
           KGRV+I YVNRK Q K+V
Sbjct: 541 KGRVKIGYVNRKPQHKIV 466

BLAST of Spg002279 vs. TAIR 10
Match: AT5G63720.1 (kokopelli )

HSP 1 Score: 87.8 bits (216), Expect = 3.0e-17
Identity = 88/263 (33.46%), Postives = 131/263 (49.81%), Query Frame = 0

Query: 314 IMRPTLLDH-------PSREVRKEQTYNKIHLATQPE----SEFTNSESESASSSSWASQ 373
           IM+PTL+D         S E   +QT +     ++ E    S+  + E+ S+S S W +Q
Sbjct: 248 IMKPTLMDQETETFDDDSSETEADQTPSATGSESEDEEVSTSQEYSGETGSSSGSEWETQ 307

Query: 374 KTLESETTDDPSSPSYQDSPPTTGSEASTRYRSSSSSSSSISTKAFRFSHRKKGSKR--A 433
              ++E+  + S P   D               S S  S+      R + R+ G +R   
Sbjct: 308 AENDTESKSESSYPPQND--------------DSVSEVSTSPPHTDRDTSREPGKQRRNV 367

Query: 434 IGRFKRLKNKLGLIFHHHHHHHHHHNSHN----FMWKQLRKIFHHTDNRKLTSNEEKSGK 493
           +GRFKR+KNK+G IFHHHHHHHHHH+ H+      W +L+  FHH        ++EKS +
Sbjct: 368 MGRFKRIKNKIGQIFHHHHHHHHHHHHHDKEKPSAWNKLQSKFHH-------KHQEKSKE 427

Query: 494 LKKTAIRSRGV-SHK--NQVGKFQALAEGLRSHVWRSKAMKKKELRGLTFGKKKGVKKLH 553
            K+    S+G+ +HK  +Q G F AL EGL  H   SK  K +         K   KK  
Sbjct: 428 RKRPMSESKGLTTHKQQHQGGHFHALVEGLVRHRKHSKKQKHQ--------LKSDAKKTE 481

Query: 554 WWKMFRRRH--GMKLSNKGRVRI 555
           WWK+ ++R   G+K+  +GRV++
Sbjct: 488 WWKLLKKRQGGGVKIPKRGRVKL 481