Sgr029982 (gene) Monk fruit (Qingpiguo) v1

Overview


Name	Sgr029982
Type	gene
Organism	Siraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Description	Tyrosine--tRNA ligase
Location	tig00153554: 1765120 .. 1765809 (+)
RNA-Seq Expression	Sgr029982
Synteny	Sgr029982

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGGGAATATCACTGCGGGACAGAGCTCATTAGGAAAGTTTTGCCTCAGGCAGATGGCGTTTATGGCTTAACATTCCCTCTTCTTCTGAAGAGCGACGGCACCAAATTTGGAAAATCCGAGGACGGCGCCATTTGGCTCTCGCCGTCGATGTTATCTCCATACAAGTTCTATCAGTATTTCTTCAGTGTCCCAGATGCTGATGTAATGAGATTTCTCAGAATTCTCACCTTTTTGGATATGAATGATATTCAGGACTTGGAAAACCAAATGGCGAGCCCCTGGTATGTGCCGAACACAGCGCAGAGGTGGCTGGCCGAAGAAGTTACCCGGTTTGTTCATGGAGAAGATGGTTTGAGTGAGGCCCTTAAGGCCACTGAAGCATTGAAACCAGGGGCAGAGACAAAGTTGGACTGGAAGACTATTGAGGGGATTGCTGAGGATGTTCCATGCTGCTCATTGGGGTATGATCAAGTGTTGAATCTTTCGGTTGTCGATCTTTCGGTTACTGCTGGTCTGCTCGAAAGCAAGTCGGCTGCCCGTCGGTTGCTGAAGCAAGGTGGACTTTATTTGAACAATAGTAGAGTGGATTCTGAAAGCAAGAGAGTTGAAGCTGTAGACATTGTAGATGGGAAAGTTCTACTTTTATCTGCAGGAAAGAAGAACAAGGTGGTTGTAAGGATATCATGA

mRNA sequence

ATGGGGGAATATCACTGCGGGACAGAGCTCATTAGGAAAGTTTTGCCTCAGGCAGATGGCGTTTATGGCTTAACATTCCCTCTTCTTCTGAAGAGCGACGGCACCAAATTTGGAAAATCCGAGGACGGCGCCATTTGGCTCTCGCCGTCGATGTTATCTCCATACAAGTTCTATCAGTATTTCTTCAGTGTCCCAGATGCTGATGTAATGAGATTTCTCAGAATTCTCACCTTTTTGGATATGAATGATATTCAGGACTTGGAAAACCAAATGGCGAGCCCCTGGTATGTGCCGAACACAGCGCAGAGGTGGCTGGCCGAAGAAGTTACCCGGTTTGTTCATGGAGAAGATGGTTTGAGTGAGGCCCTTAAGGCCACTGAAGCATTGAAACCAGGGGCAGAGACAAAGTTGGACTGGAAGACTATTGAGGGGATTGCTGAGGATGTTCCATGCTGCTCATTGGGGTATGATCAAGTGTTGAATCTTTCGGTTGTCGATCTTTCGGTTACTGCTGGTCTGCTCGAAAGCAAGTCGGCTGCCCGTCGGTTGCTGAAGCAAGGTGGACTTTATTTGAACAATAGTAGAGTGGATTCTGAAAGCAAGAGAGTTGAAGCTGTAGACATTGTAGATGGGAAAGTTCTACTTTTATCTGCAGGAAAGAAGAACAAGGTGGTTGTAAGGATATCATGA

Coding sequence (CDS)

ATGGGGGAATATCACTGCGGGACAGAGCTCATTAGGAAAGTTTTGCCTCAGGCAGATGGCGTTTATGGCTTAACATTCCCTCTTCTTCTGAAGAGCGACGGCACCAAATTTGGAAAATCCGAGGACGGCGCCATTTGGCTCTCGCCGTCGATGTTATCTCCATACAAGTTCTATCAGTATTTCTTCAGTGTCCCAGATGCTGATGTAATGAGATTTCTCAGAATTCTCACCTTTTTGGATATGAATGATATTCAGGACTTGGAAAACCAAATGGCGAGCCCCTGGTATGTGCCGAACACAGCGCAGAGGTGGCTGGCCGAAGAAGTTACCCGGTTTGTTCATGGAGAAGATGGTTTGAGTGAGGCCCTTAAGGCCACTGAAGCATTGAAACCAGGGGCAGAGACAAAGTTGGACTGGAAGACTATTGAGGGGATTGCTGAGGATGTTCCATGCTGCTCATTGGGGTATGATCAAGTGTTGAATCTTTCGGTTGTCGATCTTTCGGTTACTGCTGGTCTGCTCGAAAGCAAGTCGGCTGCCCGTCGGTTGCTGAAGCAAGGTGGACTTTATTTGAACAATAGTAGAGTGGATTCTGAAAGCAAGAGAGTTGAAGCTGTAGACATTGTAGATGGGAAAGTTCTACTTTTATCTGCAGGAAAGAAGAACAAGGTGGTTGTAAGGATATCATGA

Protein sequence

MGEYHCGTELIRKVLPQADGVYGLTFPLLLKSDGTKFGKSEDGAIWLSPSMLSPYKFYQYFFSVPDADVMRFLRILTFLDMNDIQDLENQMASPWYVPNTAQRWLAEEVTRFVHGEDGLSEALKATEALKPGAETKLDWKTIEGIAEDVPCCSLGYDQVLNLSVVDLSVTAGLLESKSAARRLLKQGGLYLNNSRVDSESKRVEAVDIVDGKVLLLSAGKKNKVVVRIS

Homology

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BLAST of Sgr029982 vs. NCBI nr
Match: XP_038892591.1 (tyrosine--tRNA ligase, chloroplastic/mitochondrial isoform X1 [Benincasa hispida] >XP_038892594.1 tyrosine--tRNA ligase, chloroplastic/mitochondrial isoform X1 [Benincasa hispida] >XP_038892599.1 tyrosine--tRNA ligase, chloroplastic/mitochondrial isoform X1 [Benincasa hispida])

HSP 1 Score: 412.9 bits (1060), Expect = 1.8e-111
Identity = 210/228 (92.11%), Postives = 217/228 (95.18%), Query Frame = 0


Query: 2   GEYHCGTELIRKVLPQADGVYGLTFPLLLKSDGTKFGKSEDGAIWLSPSMLSPYKFYQYF 61
           G    GTELIRK LPQADGVYGLTFPLLLKSDGTKFGKSEDGAIWLSPSMLSPYKFYQYF
Sbjct: 267 GNITAGTELIRKRLPQADGVYGLTFPLLLKSDGTKFGKSEDGAIWLSPSMLSPYKFYQYF 326

Query: 62  FSVPDADVMRFLRILTFLDMNDIQDLENQMASPWYVPNTAQRWLAEEVTRFVHGEDGLSE 121
           FSVPDADV+RFLRILTFLD+ +IQD+E+QMA P Y+PNTAQR LAEEVTRFVHGEDGLSE
Sbjct: 327 FSVPDADVVRFLRILTFLDIEEIQDMESQMAKPGYLPNTAQRRLAEEVTRFVHGEDGLSE 386

Query: 122 ALKATEALKPGAETKLDWKTIEGIAEDVPCCSLGYDQVLNLSVVDLSVTAGLLESKSAAR 181
           ALKATEALKPGAETKLDWK IEGIAEDVPCCSL YDQVLNLSVVDLSVT+GLLESKSAAR
Sbjct: 387 ALKATEALKPGAETKLDWKIIEGIAEDVPCCSLEYDQVLNLSVVDLSVTSGLLESKSAAR 446

Query: 182 RLLKQGGLYLNNSRVDSESKRVEAVDIVDGKVLLLSAGKKNKVVVRIS 230
           RLLKQGGLYLNNSRVDSESKRVEAVDIVDGKVLLLSAGKKNKVVVRIS
Sbjct: 447 RLLKQGGLYLNNSRVDSESKRVEAVDIVDGKVLLLSAGKKNKVVVRIS 494

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BLAST of Sgr029982 vs. NCBI nr
Match: XP_022141006.1 (tyrosine--tRNA ligase, chloroplastic/mitochondrial [Momordica charantia] >XP_022141007.1 tyrosine--tRNA ligase, chloroplastic/mitochondrial [Momordica charantia])

HSP 1 Score: 412.5 bits (1059), Expect = 2.3e-111
Identity = 208/228 (91.23%), Postives = 214/228 (93.86%), Query Frame = 0


Query: 2   GEYHCGTELIRKVLPQADGVYGLTFPLLLKSDGTKFGKSEDGAIWLSPSMLSPYKFYQYF 61
           G    GTELIRK+LP ADGVYGLTFPLLLKSDGTKFGKSEDGA+WLSPSMLSPYKFYQYF
Sbjct: 265 GNITAGTELIRKILPHADGVYGLTFPLLLKSDGTKFGKSEDGAVWLSPSMLSPYKFYQYF 324

Query: 62  FSVPDADVMRFLRILTFLDMNDIQDLENQMASPWYVPNTAQRWLAEEVTRFVHGEDGLSE 121
           FSVPDADV RFL+ILTFLDM DIQDLE+QM S  YVPNTAQR LAEEVTRFVHGEDGLSE
Sbjct: 325 FSVPDADVARFLKILTFLDMKDIQDLESQMGSSGYVPNTAQRRLAEEVTRFVHGEDGLSE 384

Query: 122 ALKATEALKPGAETKLDWKTIEGIAEDVPCCSLGYDQVLNLSVVDLSVTAGLLESKSAAR 181
           ALKATEALKPGAETKLDWKTIEGIAEDVPCCSLGYDQVLNL +VDLSVT+GLLESKSAAR
Sbjct: 385 ALKATEALKPGAETKLDWKTIEGIAEDVPCCSLGYDQVLNLLIVDLSVTSGLLESKSAAR 444

Query: 182 RLLKQGGLYLNNSRVDSESKRVEAVDIVDGKVLLLSAGKKNKVVVRIS 230
           RLLKQGGLYLNNSRVDSE KRVEA DIVDGKVLLLSAGKKNKVVVRIS
Sbjct: 445 RLLKQGGLYLNNSRVDSEGKRVEAADIVDGKVLLLSAGKKNKVVVRIS 492

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BLAST of Sgr029982 vs. NCBI nr
Match: XP_004146892.1 (tyrosine--tRNA ligase, chloroplastic/mitochondrial [Cucumis sativus] >KGN53185.1 hypothetical protein Csa_014838 [Cucumis sativus])

HSP 1 Score: 411.8 bits (1057), Expect = 4.0e-111
Identity = 210/228 (92.11%), Postives = 215/228 (94.30%), Query Frame = 0


Query: 2   GEYHCGTELIRKVLPQADGVYGLTFPLLLKSDGTKFGKSEDGAIWLSPSMLSPYKFYQYF 61
           G    GTELIRK LPQADGVYGLTFPLLLKSDGTKFGKSEDGAIWLSPSMLSPYKFYQYF
Sbjct: 266 GNITAGTELIRKRLPQADGVYGLTFPLLLKSDGTKFGKSEDGAIWLSPSMLSPYKFYQYF 325

Query: 62  FSVPDADVMRFLRILTFLDMNDIQDLENQMASPWYVPNTAQRWLAEEVTRFVHGEDGLSE 121
           FSVPDADV+RFLRILTFLD+ +IQDLE+QM  P YVPNTAQR LAEEVTRFVHGEDGL E
Sbjct: 326 FSVPDADVVRFLRILTFLDIEEIQDLESQMMKPGYVPNTAQRRLAEEVTRFVHGEDGLRE 385

Query: 122 ALKATEALKPGAETKLDWKTIEGIAEDVPCCSLGYDQVLNLSVVDLSVTAGLLESKSAAR 181
           ALKATEALKPGAETKLDWKTIEGIAEDVPCCSL YDQVLNLSVVDLSVT+GLLESKSAAR
Sbjct: 386 ALKATEALKPGAETKLDWKTIEGIAEDVPCCSLEYDQVLNLSVVDLSVTSGLLESKSAAR 445

Query: 182 RLLKQGGLYLNNSRVDSESKRVEAVDIVDGKVLLLSAGKKNKVVVRIS 230
           RLLKQGGLYLNN RVDSESKRVEAVDIVDGKVLLLSAGKKNKVVVRIS
Sbjct: 446 RLLKQGGLYLNNGRVDSESKRVEAVDIVDGKVLLLSAGKKNKVVVRIS 493

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BLAST of Sgr029982 vs. NCBI nr
Match: XP_016901583.1 (PREDICTED: tyrosine--tRNA ligase, chloroplastic/mitochondrial [Cucumis melo] >KAA0044689.1 tyrosine--tRNA ligase [Cucumis melo var. makuwa] >TYK16895.1 tyrosine--tRNA ligase [Cucumis melo var. makuwa])

HSP 1 Score: 409.5 bits (1051), Expect = 2.0e-110
Identity = 207/228 (90.79%), Postives = 214/228 (93.86%), Query Frame = 0


Query: 2   GEYHCGTELIRKVLPQADGVYGLTFPLLLKSDGTKFGKSEDGAIWLSPSMLSPYKFYQYF 61
           G    GTELIRK LPQADGVYGLTFPLLLKSDGTKFGKSEDGAIWLSPSMLSPYKFYQYF
Sbjct: 266 GNITAGTELIRKRLPQADGVYGLTFPLLLKSDGTKFGKSEDGAIWLSPSMLSPYKFYQYF 325

Query: 62  FSVPDADVMRFLRILTFLDMNDIQDLENQMASPWYVPNTAQRWLAEEVTRFVHGEDGLSE 121
           FSVPDADV+RFLRILTFLD+ +IQDLE+QM  P YVPNT QR LAEEVTRFVHGE+GLSE
Sbjct: 326 FSVPDADVVRFLRILTFLDIEEIQDLESQMTKPGYVPNTVQRRLAEEVTRFVHGENGLSE 385

Query: 122 ALKATEALKPGAETKLDWKTIEGIAEDVPCCSLGYDQVLNLSVVDLSVTAGLLESKSAAR 181
           ALKATEALKPGAETKLDWKTIEGIAEDVPCCSL YDQVLNLSVVDLSVT GLLESKSAAR
Sbjct: 386 ALKATEALKPGAETKLDWKTIEGIAEDVPCCSLEYDQVLNLSVVDLSVTTGLLESKSAAR 445

Query: 182 RLLKQGGLYLNNSRVDSESKRVEAVDIVDGKVLLLSAGKKNKVVVRIS 230
           RLLKQGGLYLNN RVDSESKRVEAVD++DGKVLLLSAGKKNKVVVRIS
Sbjct: 446 RLLKQGGLYLNNGRVDSESKRVEAVDVIDGKVLLLSAGKKNKVVVRIS 493

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BLAST of Sgr029982 vs. NCBI nr
Match: XP_038892604.1 (tyrosine--tRNA ligase, chloroplastic/mitochondrial isoform X2 [Benincasa hispida] >XP_038892611.1 tyrosine--tRNA ligase, chloroplastic/mitochondrial isoform X2 [Benincasa hispida] >XP_038892618.1 tyrosine--tRNA ligase, chloroplastic/mitochondrial isoform X2 [Benincasa hispida] >XP_038892625.1 tyrosine--tRNA ligase, chloroplastic/mitochondrial isoform X2 [Benincasa hispida])

HSP 1 Score: 404.1 bits (1037), Expect = 8.3e-109
Identity = 204/223 (91.48%), Postives = 212/223 (95.07%), Query Frame = 0


Query: 2   GEYHCGTELIRKVLPQADGVYGLTFPLLLKSDGTKFGKSEDGAIWLSPSMLSPYKFYQYF 61
           G    GTELIRK LPQADGVYGLTFPLLLKSDGTKFGKSEDGAIWLSPSMLSPYKFYQYF
Sbjct: 267 GNITAGTELIRKRLPQADGVYGLTFPLLLKSDGTKFGKSEDGAIWLSPSMLSPYKFYQYF 326

Query: 62  FSVPDADVMRFLRILTFLDMNDIQDLENQMASPWYVPNTAQRWLAEEVTRFVHGEDGLSE 121
           FSVPDADV+RFLRILTFLD+ +IQD+E+QMA P Y+PNTAQR LAEEVTRFVHGEDGLSE
Sbjct: 327 FSVPDADVVRFLRILTFLDIEEIQDMESQMAKPGYLPNTAQRRLAEEVTRFVHGEDGLSE 386

Query: 122 ALKATEALKPGAETKLDWKTIEGIAEDVPCCSLGYDQVLNLSVVDLSVTAGLLESKSAAR 181
           ALKATEALKPGAETKLDWK IEGIAEDVPCCSL YDQVLNLSVVDLSVT+GLLESKSAAR
Sbjct: 387 ALKATEALKPGAETKLDWKIIEGIAEDVPCCSLEYDQVLNLSVVDLSVTSGLLESKSAAR 446

Query: 182 RLLKQGGLYLNNSRVDSESKRVEAVDIVDGKVLLLSAGKKNKV 225
           RLLKQGGLYLNNSRVDSESKRVEAVDIVDGKVLLLSAGKKNK+
Sbjct: 447 RLLKQGGLYLNNSRVDSESKRVEAVDIVDGKVLLLSAGKKNKL 489

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BLAST of Sgr029982 vs. ExPASy Swiss-Prot
Match: Q9M876 (Tyrosine--tRNA ligase, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=EMB2768 PE=2 SV=1)

HSP 1 Score: 341.7 bits (875), Expect = 6.6e-93
Identity = 169/228 (74.12%), Postives = 195/228 (85.53%), Query Frame = 0


Query: 2   GEYHCGTELIRKVLPQADGVYGLTFPLLLKSDGTKFGKSEDGAIWLSPSMLSPYKFYQYF 61
           G    GT+LIRK+L   +  YGLTFPLLLK+DGTKFGKSEDGAIWLSPSMLSPYKFYQYF
Sbjct: 284 GNITAGTDLIRKILQAEEAAYGLTFPLLLKNDGTKFGKSEDGAIWLSPSMLSPYKFYQYF 343

Query: 62  FSVPDADVMRFLRILTFLDMNDIQDLENQMASPWYVPNTAQRWLAEEVTRFVHGEDGLSE 121
           FSVPD DV+RFL+ LTFL +++I+ LE+QM+ P YVPNTAQ  LAEEVTRFVHGE+GL E
Sbjct: 344 FSVPDVDVIRFLKTLTFLSLDEIKILEDQMSKPGYVPNTAQIKLAEEVTRFVHGEEGLKE 403

Query: 122 ALKATEALKPGAETKLDWKTIEGIAEDVPCCSLGYDQVLNLSVVDLSVTAGLLESKSAAR 181
           A+KATEAL+PGAETKLDW  IE IAED+P CSL  D+V  LS+VDLSV+AGL ESKSAAR
Sbjct: 404 AIKATEALRPGAETKLDWNLIERIAEDIPSCSLPIDRVSGLSIVDLSVSAGLFESKSAAR 463

Query: 182 RLLKQGGLYLNNSRVDSESKRVEAVDIVDGKVLLLSAGKKNKVVVRIS 230
           R+LKQGG Y+NN RVD E+KRV+  DIV+G+ L+LSAGKKNKVVVRIS
Sbjct: 464 RMLKQGGFYMNNERVDDENKRVDEEDIVEGRGLVLSAGKKNKVVVRIS 511

[Close]

BLAST of Sgr029982 vs. ExPASy Swiss-Prot
Match: Q6MC06 (Tyrosine--tRNA ligase OS=Protochlamydia amoebophila (strain UWE25) OX=264201 GN=tyrS PE=3 SV=1)

HSP 1 Score: 234.6 bits (597), Expect = 1.1e-60
Identity = 114/227 (50.22%), Postives = 157/227 (69.16%), Query Frame = 0


Query: 2   GEYHCGTELIRKVLPQADGVYGLTFPLLLKSDGTKFGKSEDGAIWLSPSMLSPYKFYQYF 61
           G    GT+LIRKV   A   YG+TFPLL KSDG KFGKSE GA+WLSP  LS Y+FYQ+ 
Sbjct: 198 GNITAGTDLIRKV--HAKPAYGITFPLLTKSDGQKFGKSEKGAVWLSPDKLSSYEFYQHL 257

Query: 62  FSVPDADVMRFLRILTFLDMNDIQDLENQMASPWYVPNTAQRWLAEEVTRFVHGEDGLSE 121
             V DADV+  +R+LTFLDM +I+  E  M    YVP TAQ+ LAEE+TR VHGE+GL  
Sbjct: 258 IRVEDADVINLMRMLTFLDMGEIRHYEQMMKEADYVPRTAQKRLAEEITRLVHGEEGLKI 317

Query: 122 ALKATEALKPGAETKLDWKTIEGIAEDVPCCSLGYDQVLNLSVVDLSVTAGLLESKSAAR 181
           A+K TE + PG++T L+   +E +A D+P C +  + VLN  ++DL V   L  SKS AR
Sbjct: 318 AIKVTEGVAPGSQTSLNADILEKLAADMPSCEMKLENVLNKKLIDLLVETKLQTSKSEAR 377

Query: 182 RLLKQGGLYLNNSRVDSESKRVEAVDIVDGKVLLLSAGKKNKVVVRI 229
           RLL+ GG+Y+NN +++ E+  +    ++  +++LL+AGKKNK+++R+
Sbjct: 378 RLLRNGGVYINNKKIEDENHIISVECLISSRLILLAAGKKNKMIIRL 422

[Close]

BLAST of Sgr029982 vs. ExPASy Swiss-Prot
Match: A5UUZ9 (Tyrosine--tRNA ligase OS=Roseiflexus sp. (strain RS-1) OX=357808 GN=tyrS PE=3 SV=1)

HSP 1 Score: 170.2 bits (430), Expect = 2.6e-41
Identity = 94/229 (41.05%), Postives = 141/229 (61.57%), Query Frame = 0


Query: 2   GEYHCGTELIRKVLPQADGVYGLTFPLLLKSDGTKFGKSEDGAIWLSPSMLSPYKFYQYF 61
           G    G ELIR+ L Q    YGL +PL+ K+DGTKFGK+E GA+WL P   SPY+FYQ++
Sbjct: 198 GNITAGIELIRRTLGQK--AYGLVYPLVTKADGTKFGKTESGAVWLDPKRTSPYRFYQFW 257

Query: 62  FSVPDADVMRFLRILTFLDMNDIQDLENQMASPWYVPNTAQRWLAEEVTRFVHGEDGLSE 121
           ++V DADV ++LR  T+L  ++I++LE   AS      TAQ+ LA EVTR VHGE  L+ 
Sbjct: 258 YNVDDADVGKYLRYFTWLSADEIEELERITASQ-PERRTAQQRLAREVTRMVHGETALAR 317

Query: 122 ALKATEALKPGAETKLDWKTIEGIAEDVPCCSLGYDQVL--NLSVVDLSVTAGLLESKSA 181
           A +A++AL  G+  +L    +  I EDVP  +L           ++D+ +  G+  SKS 
Sbjct: 318 AEEASQALFGGSLARLSAGDVIDIFEDVPSTTLPRQDFEGDGFPLIDVLLRCGIATSKSD 377

Query: 182 ARRLLKQGGLYLNNSRVDSESKRVEAVDIVDGKVLLLSAGKKNKVVVRI 229
           ARR ++ GG+Y+NN +     +R+   D +DG+ ++L  G+K   +V++
Sbjct: 378 ARRTIEGGGIYVNNIQCPDVGRRLTLNDSIDGQFIVLRKGRKQYHLVKV 423

[Close]

BLAST of Sgr029982 vs. ExPASy Swiss-Prot
Match: A7NK64 (Tyrosine--tRNA ligase OS=Roseiflexus castenholzii (strain DSM 13941 / HLO8) OX=383372 GN=tyrS PE=3 SV=1)

HSP 1 Score: 169.9 bits (429), Expect = 3.4e-41
Identity = 93/229 (40.61%), Postives = 142/229 (62.01%), Query Frame = 0


Query: 2   GEYHCGTELIRKVLPQADGVYGLTFPLLLKSDGTKFGKSEDGAIWLSPSMLSPYKFYQYF 61
           G    G ELIR+   Q    YGL +PL+ K+DGTKFGK+E GA+WL P   SPY+FYQ++
Sbjct: 198 GNITAGIELIRRTSGQK--AYGLVYPLVTKADGTKFGKTESGAVWLDPKRTSPYRFYQFW 257

Query: 62  FSVPDADVMRFLRILTFLDMNDIQDLENQMASPWYVPNTAQRWLAEEVTRFVHGEDGLSE 121
           ++V DADV ++LR  T+L   +I++LE Q+ +      TAQ+ LA EVTR VHGE  L+ 
Sbjct: 258 YNVDDADVGKYLRYFTWLSAAEIEELE-QITARQPERRTAQQRLAREVTRMVHGEAALAR 317

Query: 122 ALKATEALKPGAETKLDWKTIEGIAEDVPCCSLGYDQVL--NLSVVDLSVTAGLLESKSA 181
           A +A++AL  G+   L    +  I EDVP  +L    +    +S++D  V  G+  SKS 
Sbjct: 318 AEEASQALFGGSLASLSADDVADIFEDVPSITLPRQDLEGDGMSLIDALVRCGIATSKSD 377

Query: 182 ARRLLKQGGLYLNNSRVDSESKRVEAVDIVDGKVLLLSAGKKNKVVVRI 229
           ARR ++ GG+Y+NN +     +R+   D +DG+ ++L  G+++  ++R+
Sbjct: 378 ARRAIEGGGIYINNVQSADVGRRLTLHDSIDGQCIVLRKGRRHYHLIRL 423

[Close]

BLAST of Sgr029982 vs. ExPASy Swiss-Prot
Match: Q5FMI6 (Tyrosine--tRNA ligase OS=Lactobacillus acidophilus (strain ATCC 700396 / NCK56 / N2 / NCFM) OX=272621 GN=tyrS PE=3 SV=1)

HSP 1 Score: 160.2 bits (404), Expect = 2.7e-38
Identity = 91/228 (39.91%), Postives = 127/228 (55.70%), Query Frame = 0


Query: 2   GEYHCGTELIRKVLPQADGVYGLTFPLLLKSDGTKFGKSEDGAIWLSPSMLSPYKFYQYF 61
           G    G +LI K+       +GLT PL+LK+DGTKFGKS  GA+WL P   SPY+FYQ++
Sbjct: 197 GNITAGIDLIHKLEGSDRPAFGLTIPLMLKADGTKFGKSAGGAVWLDPEKTSPYEFYQFW 256

Query: 62  FSVPDADVMRFLRILTFLDMNDIQDL-ENQMASPWYVPNTAQRWLAEEVTRFVHGEDGLS 121
            +  D DV+++L+  TFL   +I+DL E     PW     AQ+ LAEEVT+FVHGE+GL 
Sbjct: 257 INQDDRDVVKYLKYFTFLSHEEIEDLAEKTEKEPW--KRAAQKKLAEEVTKFVHGEEGLK 316

Query: 122 EALKATEALKPGAETKLDWKTIEGIAEDVPCCSLGYDQVLNLSVVDLSVTAGLLESKSAA 181
           EA   T+AL  G    L    IE   ++ P    G ++    ++VD  V   +  SK  A
Sbjct: 317 EAQMITDALFSGDIKNLSVTQIEQGLKNAPSAEAGSEK---KNIVDFLVDTKIEPSKRQA 376

Query: 182 RRLLKQGGLYLNNSRVDSESKRVEAVDIVDGKVLLLSAGKKNKVVVRI 229
           R  +K G +Y+N  R  S    V+     DGK +++  GK+   +V I
Sbjct: 377 REDVKNGAIYVNGDREQSVDFEVDPSSAFDGKYVIIRKGKRKYTLVTI 419

[Close]

BLAST of Sgr029982 vs. ExPASy TrEMBL
Match: A0A6J1CIM7 (Tyrosine--tRNA ligase OS=Momordica charantia OX=3673 GN=LOC111011519 PE=3 SV=1)

HSP 1 Score: 412.5 bits (1059), Expect = 1.1e-111
Identity = 208/228 (91.23%), Postives = 214/228 (93.86%), Query Frame = 0


Query: 2   GEYHCGTELIRKVLPQADGVYGLTFPLLLKSDGTKFGKSEDGAIWLSPSMLSPYKFYQYF 61
           G    GTELIRK+LP ADGVYGLTFPLLLKSDGTKFGKSEDGA+WLSPSMLSPYKFYQYF
Sbjct: 265 GNITAGTELIRKILPHADGVYGLTFPLLLKSDGTKFGKSEDGAVWLSPSMLSPYKFYQYF 324

Query: 62  FSVPDADVMRFLRILTFLDMNDIQDLENQMASPWYVPNTAQRWLAEEVTRFVHGEDGLSE 121
           FSVPDADV RFL+ILTFLDM DIQDLE+QM S  YVPNTAQR LAEEVTRFVHGEDGLSE
Sbjct: 325 FSVPDADVARFLKILTFLDMKDIQDLESQMGSSGYVPNTAQRRLAEEVTRFVHGEDGLSE 384

Query: 122 ALKATEALKPGAETKLDWKTIEGIAEDVPCCSLGYDQVLNLSVVDLSVTAGLLESKSAAR 181
           ALKATEALKPGAETKLDWKTIEGIAEDVPCCSLGYDQVLNL +VDLSVT+GLLESKSAAR
Sbjct: 385 ALKATEALKPGAETKLDWKTIEGIAEDVPCCSLGYDQVLNLLIVDLSVTSGLLESKSAAR 444

Query: 182 RLLKQGGLYLNNSRVDSESKRVEAVDIVDGKVLLLSAGKKNKVVVRIS 230
           RLLKQGGLYLNNSRVDSE KRVEA DIVDGKVLLLSAGKKNKVVVRIS
Sbjct: 445 RLLKQGGLYLNNSRVDSEGKRVEAADIVDGKVLLLSAGKKNKVVVRIS 492

[Close]

BLAST of Sgr029982 vs. ExPASy TrEMBL
Match: A0A0A0KU70 (Tyrosine--tRNA ligase OS=Cucumis sativus OX=3659 GN=Csa_4G025150 PE=3 SV=1)

HSP 1 Score: 411.8 bits (1057), Expect = 1.9e-111
Identity = 210/228 (92.11%), Postives = 215/228 (94.30%), Query Frame = 0


Query: 2   GEYHCGTELIRKVLPQADGVYGLTFPLLLKSDGTKFGKSEDGAIWLSPSMLSPYKFYQYF 61
           G    GTELIRK LPQADGVYGLTFPLLLKSDGTKFGKSEDGAIWLSPSMLSPYKFYQYF
Sbjct: 266 GNITAGTELIRKRLPQADGVYGLTFPLLLKSDGTKFGKSEDGAIWLSPSMLSPYKFYQYF 325

Query: 62  FSVPDADVMRFLRILTFLDMNDIQDLENQMASPWYVPNTAQRWLAEEVTRFVHGEDGLSE 121
           FSVPDADV+RFLRILTFLD+ +IQDLE+QM  P YVPNTAQR LAEEVTRFVHGEDGL E
Sbjct: 326 FSVPDADVVRFLRILTFLDIEEIQDLESQMMKPGYVPNTAQRRLAEEVTRFVHGEDGLRE 385

Query: 122 ALKATEALKPGAETKLDWKTIEGIAEDVPCCSLGYDQVLNLSVVDLSVTAGLLESKSAAR 181
           ALKATEALKPGAETKLDWKTIEGIAEDVPCCSL YDQVLNLSVVDLSVT+GLLESKSAAR
Sbjct: 386 ALKATEALKPGAETKLDWKTIEGIAEDVPCCSLEYDQVLNLSVVDLSVTSGLLESKSAAR 445

Query: 182 RLLKQGGLYLNNSRVDSESKRVEAVDIVDGKVLLLSAGKKNKVVVRIS 230
           RLLKQGGLYLNN RVDSESKRVEAVDIVDGKVLLLSAGKKNKVVVRIS
Sbjct: 446 RLLKQGGLYLNNGRVDSESKRVEAVDIVDGKVLLLSAGKKNKVVVRIS 493

[Close]

BLAST of Sgr029982 vs. ExPASy TrEMBL
Match: A0A5D3CZD9 (Tyrosine--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold130G00290 PE=3 SV=1)

HSP 1 Score: 409.5 bits (1051), Expect = 9.5e-111
Identity = 207/228 (90.79%), Postives = 214/228 (93.86%), Query Frame = 0


Query: 2   GEYHCGTELIRKVLPQADGVYGLTFPLLLKSDGTKFGKSEDGAIWLSPSMLSPYKFYQYF 61
           G    GTELIRK LPQADGVYGLTFPLLLKSDGTKFGKSEDGAIWLSPSMLSPYKFYQYF
Sbjct: 266 GNITAGTELIRKRLPQADGVYGLTFPLLLKSDGTKFGKSEDGAIWLSPSMLSPYKFYQYF 325

Query: 62  FSVPDADVMRFLRILTFLDMNDIQDLENQMASPWYVPNTAQRWLAEEVTRFVHGEDGLSE 121
           FSVPDADV+RFLRILTFLD+ +IQDLE+QM  P YVPNT QR LAEEVTRFVHGE+GLSE
Sbjct: 326 FSVPDADVVRFLRILTFLDIEEIQDLESQMTKPGYVPNTVQRRLAEEVTRFVHGENGLSE 385

Query: 122 ALKATEALKPGAETKLDWKTIEGIAEDVPCCSLGYDQVLNLSVVDLSVTAGLLESKSAAR 181
           ALKATEALKPGAETKLDWKTIEGIAEDVPCCSL YDQVLNLSVVDLSVT GLLESKSAAR
Sbjct: 386 ALKATEALKPGAETKLDWKTIEGIAEDVPCCSLEYDQVLNLSVVDLSVTTGLLESKSAAR 445

Query: 182 RLLKQGGLYLNNSRVDSESKRVEAVDIVDGKVLLLSAGKKNKVVVRIS 230
           RLLKQGGLYLNN RVDSESKRVEAVD++DGKVLLLSAGKKNKVVVRIS
Sbjct: 446 RLLKQGGLYLNNGRVDSESKRVEAVDVIDGKVLLLSAGKKNKVVVRIS 493

[Close]

BLAST of Sgr029982 vs. ExPASy TrEMBL
Match: A0A1S4E026 (Tyrosine--tRNA ligase OS=Cucumis melo OX=3656 GN=LOC103494447 PE=3 SV=1)

HSP 1 Score: 409.5 bits (1051), Expect = 9.5e-111
Identity = 207/228 (90.79%), Postives = 214/228 (93.86%), Query Frame = 0


Query: 2   GEYHCGTELIRKVLPQADGVYGLTFPLLLKSDGTKFGKSEDGAIWLSPSMLSPYKFYQYF 61
           G    GTELIRK LPQADGVYGLTFPLLLKSDGTKFGKSEDGAIWLSPSMLSPYKFYQYF
Sbjct: 266 GNITAGTELIRKRLPQADGVYGLTFPLLLKSDGTKFGKSEDGAIWLSPSMLSPYKFYQYF 325

Query: 62  FSVPDADVMRFLRILTFLDMNDIQDLENQMASPWYVPNTAQRWLAEEVTRFVHGEDGLSE 121
           FSVPDADV+RFLRILTFLD+ +IQDLE+QM  P YVPNT QR LAEEVTRFVHGE+GLSE
Sbjct: 326 FSVPDADVVRFLRILTFLDIEEIQDLESQMTKPGYVPNTVQRRLAEEVTRFVHGENGLSE 385

Query: 122 ALKATEALKPGAETKLDWKTIEGIAEDVPCCSLGYDQVLNLSVVDLSVTAGLLESKSAAR 181
           ALKATEALKPGAETKLDWKTIEGIAEDVPCCSL YDQVLNLSVVDLSVT GLLESKSAAR
Sbjct: 386 ALKATEALKPGAETKLDWKTIEGIAEDVPCCSLEYDQVLNLSVVDLSVTTGLLESKSAAR 445

Query: 182 RLLKQGGLYLNNSRVDSESKRVEAVDIVDGKVLLLSAGKKNKVVVRIS 230
           RLLKQGGLYLNN RVDSESKRVEAVD++DGKVLLLSAGKKNKVVVRIS
Sbjct: 446 RLLKQGGLYLNNGRVDSESKRVEAVDVIDGKVLLLSAGKKNKVVVRIS 493

[Close]

BLAST of Sgr029982 vs. ExPASy TrEMBL
Match: A0A6J1FW55 (Tyrosine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111447426 PE=3 SV=1)

HSP 1 Score: 400.6 bits (1028), Expect = 4.4e-108
Identity = 200/228 (87.72%), Postives = 215/228 (94.30%), Query Frame = 0


Query: 2   GEYHCGTELIRKVLPQADGVYGLTFPLLLKSDGTKFGKSEDGAIWLSPSMLSPYKFYQYF 61
           G    GTELIRK+LPQADGVYGLTFPLLLKSDGTKFGKS DGAIWLSPS LSPYKFYQYF
Sbjct: 264 GNITAGTELIRKILPQADGVYGLTFPLLLKSDGTKFGKSVDGAIWLSPSKLSPYKFYQYF 323

Query: 62  FSVPDADVMRFLRILTFLDMNDIQDLENQMASPWYVPNTAQRWLAEEVTRFVHGEDGLSE 121
           FSVPDADV+RFLRILTFLD+ +I+DLE+QMASP YVPNTAQR LAEEVTRFVHGEDGL+E
Sbjct: 324 FSVPDADVIRFLRILTFLDIEEIRDLESQMASPGYVPNTAQRRLAEEVTRFVHGEDGLNE 383

Query: 122 ALKATEALKPGAETKLDWKTIEGIAEDVPCCSLGYDQVLNLSVVDLSVTAGLLESKSAAR 181
           AL+ATEALKPGAETKLDWKTI GI EDVPCCSL YDQVLNLSVVDL+V +GLLESKSAAR
Sbjct: 384 ALEATEALKPGAETKLDWKTIVGIGEDVPCCSLEYDQVLNLSVVDLAVMSGLLESKSAAR 443

Query: 182 RLLKQGGLYLNNSRVDSESKRVEAVDIVDGKVLLLSAGKKNKVVVRIS 230
           RLLKQGGLYLNNSRVDSESK++EA+DI+DGKVLLLSAGKKNK+VVRIS
Sbjct: 444 RLLKQGGLYLNNSRVDSESKKIEAIDIIDGKVLLLSAGKKNKMVVRIS 491

[Close]

BLAST of Sgr029982 vs. TAIR 10
Match: AT3G02660.1 (Tyrosyl-tRNA synthetase, class Ib, bacterial/mitochondrial )

HSP 1 Score: 341.7 bits (875), Expect = 4.7e-94
Identity = 169/228 (74.12%), Postives = 195/228 (85.53%), Query Frame = 0


Query: 2   GEYHCGTELIRKVLPQADGVYGLTFPLLLKSDGTKFGKSEDGAIWLSPSMLSPYKFYQYF 61
           G    GT+LIRK+L   +  YGLTFPLLLK+DGTKFGKSEDGAIWLSPSMLSPYKFYQYF
Sbjct: 284 GNITAGTDLIRKILQAEEAAYGLTFPLLLKNDGTKFGKSEDGAIWLSPSMLSPYKFYQYF 343

Query: 62  FSVPDADVMRFLRILTFLDMNDIQDLENQMASPWYVPNTAQRWLAEEVTRFVHGEDGLSE 121
           FSVPD DV+RFL+ LTFL +++I+ LE+QM+ P YVPNTAQ  LAEEVTRFVHGE+GL E
Sbjct: 344 FSVPDVDVIRFLKTLTFLSLDEIKILEDQMSKPGYVPNTAQIKLAEEVTRFVHGEEGLKE 403

Query: 122 ALKATEALKPGAETKLDWKTIEGIAEDVPCCSLGYDQVLNLSVVDLSVTAGLLESKSAAR 181
           A+KATEAL+PGAETKLDW  IE IAED+P CSL  D+V  LS+VDLSV+AGL ESKSAAR
Sbjct: 404 AIKATEALRPGAETKLDWNLIERIAEDIPSCSLPIDRVSGLSIVDLSVSAGLFESKSAAR 463

Query: 182 RLLKQGGLYLNNSRVDSESKRVEAVDIVDGKVLLLSAGKKNKVVVRIS 230
           R+LKQGG Y+NN RVD E+KRV+  DIV+G+ L+LSAGKKNKVVVRIS
Sbjct: 464 RMLKQGGFYMNNERVDDENKRVDEEDIVEGRGLVLSAGKKNKVVVRIS 511

The following BLAST results are available for this feature:

BLAST of Sgr029982 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038892591.1	1.8e-111	92.11	tyrosine--tRNA ligase, chloroplastic/mitochondrial isoform X1 [Benincasa hispida...	[more]
XP_022141006.1	2.3e-111	91.23	tyrosine--tRNA ligase, chloroplastic/mitochondrial [Momordica charantia] >XP_022...	[more]
XP_004146892.1	4.0e-111	92.11	tyrosine--tRNA ligase, chloroplastic/mitochondrial [Cucumis sativus] >KGN53185.1...	[more]
XP_016901583.1	2.0e-110	90.79	PREDICTED: tyrosine--tRNA ligase, chloroplastic/mitochondrial [Cucumis melo] >KA...	[more]
XP_038892604.1	8.3e-109	91.48	tyrosine--tRNA ligase, chloroplastic/mitochondrial isoform X2 [Benincasa hispida...	[more]

BLAST of Sgr029982 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9M876	6.6e-93	74.12	Tyrosine--tRNA ligase, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=37...	[more]
Q6MC06	1.1e-60	50.22	Tyrosine--tRNA ligase OS=Protochlamydia amoebophila (strain UWE25) OX=264201 GN=...	[more]
A5UUZ9	2.6e-41	41.05	Tyrosine--tRNA ligase OS=Roseiflexus sp. (strain RS-1) OX=357808 GN=tyrS PE=3 SV...	[more]
A7NK64	3.4e-41	40.61	Tyrosine--tRNA ligase OS=Roseiflexus castenholzii (strain DSM 13941 / HLO8) OX=3...	[more]
Q5FMI6	2.7e-38	39.91	Tyrosine--tRNA ligase OS=Lactobacillus acidophilus (strain ATCC 700396 / NCK56 /...	[more]

BLAST of Sgr029982 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CIM7	1.1e-111	91.23	Tyrosine--tRNA ligase OS=Momordica charantia OX=3673 GN=LOC111011519 PE=3 SV=1	[more]
A0A0A0KU70	1.9e-111	92.11	Tyrosine--tRNA ligase OS=Cucumis sativus OX=3659 GN=Csa_4G025150 PE=3 SV=1	[more]
A0A5D3CZD9	9.5e-111	90.79	Tyrosine--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13...	[more]
A0A1S4E026	9.5e-111	90.79	Tyrosine--tRNA ligase OS=Cucumis melo OX=3656 GN=LOC103494447 PE=3 SV=1	[more]
A0A6J1FW55	4.4e-108	87.72	Tyrosine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111447426 PE=3 SV=1	[more]

BLAST of Sgr029982 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G02660.1	4.7e-94	74.12	Tyrosyl-tRNA synthetase, class Ib, bacterial/mitochondrial	[more]

InterPro

Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002307	Tyrosine-tRNA ligase	TIGRFAM	TIGR00234	TIGR00234	coord: 7..227 e-value: 1.3E-38 score: 131.1
IPR002942	RNA-binding S4 domain	PFAM	PF01479	S4	coord: 170..208 e-value: 1.5E-6 score: 27.8
IPR002942	RNA-binding S4 domain	CDD	cd00165	S4	coord: 169..228 e-value: 2.7129E-7 score: 44.5478
IPR002305	Aminoacyl-tRNA synthetase, class Ic	PFAM	PF00579	tRNA-synt_1b	coord: 7..128 e-value: 3.0E-20 score: 72.7
None	No IPR available	GENE3D	1.10.240.10		coord: 29..131 e-value: 1.5E-34 score: 120.4
None	No IPR available	PROSITE	PS50889	S4	coord: 162..228 score: 11.94722
None	No IPR available	SUPERFAMILY	52374	Nucleotidylyl transferase	coord: 17..128
None	No IPR available	SUPERFAMILY	55174	Alpha-L RNA-binding motif	coord: 131..227
IPR036986	RNA-binding S4 domain superfamily	GENE3D	3.10.290.10		coord: 132..229 e-value: 3.0E-22 score: 80.3
IPR024088	Tyrosine-tRNA ligase, bacterial-type	PANTHER	PTHR11766	TYROSYL-TRNA SYNTHETASE	coord: 1..228

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Sgr029982.1	Sgr029982.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0000165	MAPK cascade
biological_process	GO:0006468	protein phosphorylation
biological_process	GO:0006437	tyrosyl-tRNA aminoacylation
biological_process	GO:0006418	tRNA aminoacylation for protein translation
cellular_component	GO:0005829	cytosol
cellular_component	GO:0005739	mitochondrion
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004707	MAP kinase activity
molecular_function	GO:0003723	RNA binding
molecular_function	GO:0004831	tyrosine-tRNA ligase activity
molecular_function	GO:0004812	aminoacyl-tRNA ligase activity
molecular_function	GO:0000166	nucleotide binding