Sgr028859 (gene) Monk fruit (Qingpiguo) v1

BLAST of Sgr028859 vs. NCBI nr
Match: XP_022138947.1 (mediator of RNA polymerase II transcription subunit 15a-like isoform X1 [Momordica charantia])

HSP 1 Score: 573.2 bits (1476), Expect = 1.7e-159
Identity = 299/388 (77.06%), Postives = 320/388 (82.47%), Query Frame = 0

Query: 1   MLTGLPENGRGDELNTSLCSFFILRHLLLYFLQQTDCEE-VWDDYFFCSQRGNMSHQTVN 60
           M+ GLPENGR                         DCEE  WDD+ F SQRG +SHQ VN
Sbjct: 1   MVPGLPENGR------------------------ADCEEDAWDDFLF-SQRGKVSHQIVN 60

Query: 61  DAVQQEKAFIISTPGMSPSALLEEVSESDRENEICCVAPLQRLLKMVNIMSPEALSASVS 120
           DAVQ+EK FIISTPGMSPSALLEEVSESDR+N+IC V PLQRLLKMVN+MSP+ALSAS+S
Sbjct: 61  DAVQEEKPFIISTPGMSPSALLEEVSESDRDNDICSVTPLQRLLKMVNVMSPKALSASIS 120

Query: 121 DIGSIVCMTDSVASSAPAPLNKSKAAIREDLVGTVASRLRKGCCSRFGGTFGSRRTRHCR 180
           DI SIVCMTD VASS  APLNKSKA I EDLVGTV SRLRK  CS FGG+ GSRRTRHCR
Sbjct: 121 DIDSIVCMTDRVASS--APLNKSKAEISEDLVGTVTSRLRKR-CSIFGGSTGSRRTRHCR 180

Query: 181 SARMYASSRRLMDCESYDLNSIFTKRSRVETPEHSHWRGVSLPVKQSLLEEIRKINEQLI 240
           + RMY SSRR+M CESYDLNS FT+RSR+ETPEHSHWRG SLP KQ LLEEIRKINEQLI
Sbjct: 181 NTRMYTSSRRMMYCESYDLNSTFTERSRIETPEHSHWRGFSLPFKQYLLEEIRKINEQLI 240

Query: 241 DTVVSICDKDMITSDTADANDRSEGIIVECSFVAMRINSNLISQQTSALQLAVHPLKLLV 300
           DTVVS+CDKDMIT+D A  NDRSEGIIVECSF AMRI+SN ISQQ SALQL VHPLKLLV
Sbjct: 241 DTVVSVCDKDMITTDNAATNDRSEGIIVECSFAAMRISSNKISQQASALQLTVHPLKLLV 300

Query: 301 PKGYPHCSPIFEKLHVEACPTDCEGLSFKAREKFDMSVRSLPHPFSISEVARTWDVCARE 360
           PKGYPHCSPIF KL +EACPTDCE LS K REKF+M VRSLPHPFSISEVARTWD CARE
Sbjct: 301 PKGYPHCSPIFNKLKLEACPTDCEELSLKTREKFNMCVRSLPHPFSISEVARTWDACARE 360

Query: 361 VVSEYAQRNGGGSFSSKYGSWEDCLHAA 388
           VVSE+AQRNGGGSF+SKYGSWEDCLH A
Sbjct: 361 VVSEFAQRNGGGSFTSKYGSWEDCLHHA 360

BLAST of Sgr028859 vs. NCBI nr
Match: KAG6601093.1 (Mediator of RNA polymerase II transcription subunit 15a, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 461.8 bits (1187), Expect = 5.6e-126
Identity = 242/331 (73.11%), Postives = 279/331 (84.29%), Query Frame = 0

Query: 58  VNDAVQQEKAFIISTPGMSPSALLEEVSESDRENEICCVAPLQRLLKMVNIMSPEALSAS 117
           VNDA Q EKAFI+STPGMSPS+LLEEV++SDR+N+ CCV PLQRL+KMVN+MSP+ALSAS
Sbjct: 101 VNDAAQAEKAFIVSTPGMSPSSLLEEVTDSDRDNDKCCVTPLQRLIKMVNVMSPKALSAS 160

Query: 118 VSDIGSIVCMTDSVASSAPAPLNKSKAAIREDLVGTVASRLRKGCCSRFGGTFGSRRTRH 177
           VSDI SIVCMTD V+SS  APLNKSK A+  DL GTVASRLR+  CSRFGGT GSRRTRH
Sbjct: 161 VSDIDSIVCMTDRVSSS--APLNKSKGAVGMDLAGTVASRLRRK-CSRFGGTIGSRRTRH 220

Query: 178 CRSARMYASSRRLMDCESYDLNSIFTKRSR-VETPEHSHWRGVSLPVKQSLLEEIRKINE 237
            R          LMDCE +DL+S+FT+RSR +ETP+HS+W G SLPVKQSLLEEIR+INE
Sbjct: 221 YRC---------LMDCEGFDLSSLFTQRSRIIETPKHSNWHGFSLPVKQSLLEEIRRINE 280

Query: 238 QLIDTVVSICDKDMITSDTADANDRSEGIIVECSFVAMRINSNLISQQTSALQLAVHPLK 297
           QLI T VSICDKDM+T+D      RSEGI+VECSF A+   S+LIS+QTSA+QLA+HPLK
Sbjct: 281 QLIYTEVSICDKDMVTTDPGATIGRSEGILVECSFGAVCTLSHLISRQTSAIQLAMHPLK 340

Query: 298 LLVPKGYPHCSPIFEKLHVEACPTDCEGLSFKAREKFDMSVRSLPHPFSISEVARTWDVC 357
           LLVP GYPH SPIF+   +EACPT CE LS +AREKF+MSVRSL HPFSISEVARTWD+C
Sbjct: 341 LLVPIGYPHISPIFK---MEACPTGCEDLSLQAREKFNMSVRSLAHPFSISEVARTWDMC 400

Query: 358 AREVVSEYAQRNGGGSFSSKYGSWEDCLHAA 388
           AR+V+SEYA++NGGGSFSSKYGSWEDCLH A
Sbjct: 401 ARDVISEYARQNGGGSFSSKYGSWEDCLHTA 416

BLAST of Sgr028859 vs. NCBI nr
Match: XP_022956517.1 (mediator of RNA polymerase II transcription subunit 15a-like [Cucurbita moschata] >XP_022956518.1 mediator of RNA polymerase II transcription subunit 15a-like [Cucurbita moschata])

HSP 1 Score: 461.5 bits (1186), Expect = 7.4e-126
Identity = 242/331 (73.11%), Postives = 278/331 (83.99%), Query Frame = 0

Query: 58  VNDAVQQEKAFIISTPGMSPSALLEEVSESDRENEICCVAPLQRLLKMVNIMSPEALSAS 117
           VNDA Q EKAFI+STPGMSPS+LLEEV++SDR+N+ CCV PLQRL+KMVN+MSP+ALSAS
Sbjct: 101 VNDAAQAEKAFIVSTPGMSPSSLLEEVTDSDRDNDKCCVTPLQRLIKMVNVMSPKALSAS 160

Query: 118 VSDIGSIVCMTDSVASSAPAPLNKSKAAIREDLVGTVASRLRKGCCSRFGGTFGSRRTRH 177
           VSDI SIVCMTD V+SS  APLNKSK A+  DL GTVASRLR+  CSRFGGT GSRRTRH
Sbjct: 161 VSDIDSIVCMTDRVSSS--APLNKSKGAVGMDLAGTVASRLRRK-CSRFGGTIGSRRTRH 220

Query: 178 CRSARMYASSRRLMDCESYDLNSIFTKRSR-VETPEHSHWRGVSLPVKQSLLEEIRKINE 237
            R          LMDCE +DL+S+FT+RSR +ETP+HS+W G SLPVKQSLLEEIR+INE
Sbjct: 221 YRC---------LMDCEGFDLSSLFTQRSRIIETPKHSNWHGFSLPVKQSLLEEIRRINE 280

Query: 238 QLIDTVVSICDKDMITSDTADANDRSEGIIVECSFVAMRINSNLISQQTSALQLAVHPLK 297
           QLI T VSICDKDM+T+D      RSEGI+VECSF A+   S+LIS QTSA+QLA+HPLK
Sbjct: 281 QLIYTEVSICDKDMVTTDPGATIGRSEGILVECSFGAVCTLSHLISHQTSAIQLAMHPLK 340

Query: 298 LLVPKGYPHCSPIFEKLHVEACPTDCEGLSFKAREKFDMSVRSLPHPFSISEVARTWDVC 357
           LLVP GYPH SPIF+   +EACPT CE LS +AREKF+MSVRSL HPFSISEVARTWD+C
Sbjct: 341 LLVPIGYPHISPIFK---MEACPTGCEDLSLQAREKFNMSVRSLAHPFSISEVARTWDMC 400

Query: 358 AREVVSEYAQRNGGGSFSSKYGSWEDCLHAA 388
           AR+V+SEYA++NGGGSFSSKYGSWEDCLH A
Sbjct: 401 ARDVISEYARQNGGGSFSSKYGSWEDCLHTA 416

BLAST of Sgr028859 vs. NCBI nr
Match: XP_023526658.1 (mediator of RNA polymerase II transcription subunit 15a-like [Cucurbita pepo subsp. pepo] >XP_023526666.1 mediator of RNA polymerase II transcription subunit 15a-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 461.1 bits (1185), Expect = 9.6e-126
Identity = 243/331 (73.41%), Postives = 279/331 (84.29%), Query Frame = 0

Query: 58  VNDAVQQEKAFIISTPGMSPSALLEEVSESDRENEICCVAPLQRLLKMVNIMSPEALSAS 117
           VNDA Q EKAFI+STPGMSPS+LLEEV++SDR+N+ CCV PLQRL+KMVN+MSP+ALSAS
Sbjct: 101 VNDAAQAEKAFIVSTPGMSPSSLLEEVTDSDRDNDKCCVTPLQRLIKMVNVMSPKALSAS 160

Query: 118 VSDIGSIVCMTDSVASSAPAPLNKSKAAIREDLVGTVASRLRKGCCSRFGGTFGSRRTRH 177
           VSDI SIVCMTD V SS  APLNKSK A+  DL GTVASRLR+  CSRFGGT GSRRTRH
Sbjct: 161 VSDIDSIVCMTDRVPSS--APLNKSKGAVGMDLAGTVASRLRRK-CSRFGGTNGSRRTRH 220

Query: 178 CRSARMYASSRRLMDCESYDLNSIFTKRSRV-ETPEHSHWRGVSLPVKQSLLEEIRKINE 237
            R          LMDCE +DL+S+FT+RSR+ ETP+HS+W G SLPVKQSLLEEIR+INE
Sbjct: 221 YRC---------LMDCEGFDLSSLFTQRSRITETPKHSNWHGFSLPVKQSLLEEIRRINE 280

Query: 238 QLIDTVVSICDKDMITSDTADANDRSEGIIVECSFVAMRINSNLISQQTSALQLAVHPLK 297
           QLI T VSICDKDM+T+D+A    RSEGI+VECSF A+   S+LIS QTSA+QLA+HPLK
Sbjct: 281 QLIYTEVSICDKDMVTTDSAATIGRSEGILVECSFGAVCTLSHLISPQTSAVQLAMHPLK 340

Query: 298 LLVPKGYPHCSPIFEKLHVEACPTDCEGLSFKAREKFDMSVRSLPHPFSISEVARTWDVC 357
           LLVP GYPH SPIF+   +EACPT CE LS +AREKF+MSVRSL HPFSISEVARTWD+C
Sbjct: 341 LLVPIGYPHISPIFK---MEACPTGCEDLSLQAREKFNMSVRSLAHPFSISEVARTWDMC 400

Query: 358 AREVVSEYAQRNGGGSFSSKYGSWEDCLHAA 388
           AR+V+SEYA++NGGGSFSSKYGSWEDCLH A
Sbjct: 401 ARDVISEYARQNGGGSFSSKYGSWEDCLHTA 416

BLAST of Sgr028859 vs. NCBI nr
Match: XP_022984739.1 (mediator of RNA polymerase II transcription subunit 15a-like [Cucurbita maxima])

HSP 1 Score: 457.6 bits (1176), Expect = 1.1e-124
Identity = 240/331 (72.51%), Postives = 278/331 (83.99%), Query Frame = 0

Query: 58  VNDAVQQEKAFIISTPGMSPSALLEEVSESDRENEICCVAPLQRLLKMVNIMSPEALSAS 117
           VNDA Q EKAFI+STPGMSPS+LLEEV++SDR+N+ CCV PLQRL+KMVN+MSP+ALSAS
Sbjct: 101 VNDAAQAEKAFIVSTPGMSPSSLLEEVTDSDRDNDKCCVTPLQRLIKMVNVMSPKALSAS 160

Query: 118 VSDIGSIVCMTDSVASSAPAPLNKSKAAIREDLVGTVASRLRKGCCSRFGGTFGSRRTRH 177
           VSDI SIVCMTD V+SS  APLNKSK A+  DL GTVASRLR+  CSRFGGT GSRRTRH
Sbjct: 161 VSDIDSIVCMTDRVSSS--APLNKSKGAVGMDLAGTVASRLRRK-CSRFGGTIGSRRTRH 220

Query: 178 CRSARMYASSRRLMDCESYDLNSIFTKRSR-VETPEHSHWRGVSLPVKQSLLEEIRKINE 237
            R          LMDCE +DL+S+FT+RSR +ETP+HS+W G SLPVKQSLLEEIR+INE
Sbjct: 221 YRC---------LMDCEGFDLSSLFTQRSRIIETPKHSNWHGFSLPVKQSLLEEIRRINE 280

Query: 238 QLIDTVVSICDKDMITSDTADANDRSEGIIVECSFVAMRINSNLISQQTSALQLAVHPLK 297
           QLI T VSICDKDM+T+D A    +SEGI+VECSF A+   S+LIS QTSA+QLA+ PLK
Sbjct: 281 QLIYTEVSICDKDMVTTDPAATIGKSEGILVECSFGAVCTLSHLISHQTSAIQLAMQPLK 340

Query: 298 LLVPKGYPHCSPIFEKLHVEACPTDCEGLSFKAREKFDMSVRSLPHPFSISEVARTWDVC 357
           LLVP GYPH SPIF+   +EACPT CE LS +AREKF+MSVRSL HPFSISE+ARTWD+C
Sbjct: 341 LLVPIGYPHISPIFK---MEACPTGCEDLSLQAREKFNMSVRSLAHPFSISEMARTWDMC 400

Query: 358 AREVVSEYAQRNGGGSFSSKYGSWEDCLHAA 388
           AR+V+SEYA++NGGGSFSSKYGSWEDCLH A
Sbjct: 401 ARDVISEYARQNGGGSFSSKYGSWEDCLHTA 416

BLAST of Sgr028859 vs. ExPASy Swiss-Prot
Match: F4I171 (Mediator of RNA polymerase II transcription subunit 15a OS=Arabidopsis thaliana OX=3702 GN=MED15A PE=1 SV=1)

HSP 1 Score: 187.6 bits (475), Expect = 2.7e-46
Identity = 133/363 (36.64%), Postives = 201/363 (55.37%), Query Frame = 0

Query: 51   GNMSHQTVNDAVQQEKAFIISTPGMSPSALLEEVSESDRE----------NEICCVAPLQ 110
            GN++ Q         ++  I TPG+S S LL+E +  D                   P++
Sbjct: 989  GNIARQQATGMQGVVQSLAIGTPGISASPLLQEFTSPDGNILNSSTITSGKPSATELPIE 1048

Query: 111  RLLKMVNIMSPEALSASVSDIGSIVCMTDSVASSAPAPLNKSKAAIREDLVGTVASRLR- 170
            RL++ V  +SP+ALS++VSDIGS+V M D +A SAP   N S+A++ EDLV     RL+ 
Sbjct: 1049 RLIRAVKSISPQALSSAVSDIGSVVSMVDRIAGSAPG--NGSRASVGEDLVAMTKCRLQA 1108

Query: 171  KGCCSRFGGTFGSRRTRHCRSARMYASS---------RRLMDCESYDLNSIFT---KRSR 230
            +   ++ G     +  RH  +  +  +S         ++    E+ DL S  T   K++R
Sbjct: 1109 RNFMTQEGMMATKKMKRHTTAMPLSVASLGGSVGDNYKQFAGSETSDLESTATSDGKKAR 1168

Query: 231  VETPEHSHWRGVSLPVKQSLLEEIRKINEQLIDTVVSICDKDMIT--SDTADANDRSEGI 290
             ET EH            +LLEEI++IN++LIDTVV I D +     S+ A ++   EG 
Sbjct: 1169 TET-EH------------ALLEEIKEINQRLIDTVVEISDDEDAADPSEVAISSIGCEGT 1228

Query: 291  IVECSFVAMRINSNLISQQTSALQLAVHPLKLLVPKGYPHCSP-IFEKLHVEACPTDCEG 350
             V  SF+A+ ++  L +  +S     + PL+LLVP  YP+ SP + +KL VE    + E 
Sbjct: 1229 TVRFSFIAVSLSPALKAHLSSTQMSPIQPLRLLVPCSYPNGSPSLLDKLPVETSKEN-ED 1288

Query: 351  LSFKAREKFDMSVRSLPHPFSISEVARTWDVCAREVVSEYAQRNGGGSFSSKYGSWEDCL 388
            LS KA  +F++ +RSL  P S+ ++A+TWD CAR V+ EYAQ+ GGG+FSSKYG+WE  +
Sbjct: 1289 LSSKAMARFNILLRSLSQPMSLKDIAKTWDACARAVICEYAQQFGGGTFSSKYGTWEKYV 1335

BLAST of Sgr028859 vs. ExPASy Swiss-Prot
Match: Q9SHV7 (Probable mediator of RNA polymerase II transcription subunit 15c OS=Arabidopsis thaliana OX=3702 GN=MED15C PE=3 SV=1)

HSP 1 Score: 128.3 bits (321), Expect = 1.9e-28
Identity = 92/301 (30.56%), Postives = 150/301 (49.83%), Query Frame = 0

Query: 98  PLQRLLKMVNIMSPEALSASVSDIGSIVCMTDSVASSAPAPLNKSKAAIREDLVGTVASR 157
           P+ RL+K     SP++L+ SVS+I S++ M D +  S P+    S+A + EDL     S 
Sbjct: 659 PIDRLIKAFQAASPKSLAESVSEISSVISMVDMIGGSFPSS-GGSRAGLGEDL-----SE 718

Query: 158 LRKGCCSRFGGTFGSRRTRHCRSARMYASSRRLMDCESYDLNSIFTKRSRVETPEHSHWR 217
             +   +        R  R         SS+     +SY+   + +  S V +   S  +
Sbjct: 719 RTRNFTTHEETNLSKRMKRSINIVPPDMSSQ----IDSYE--QLSSLESEVVSTTSSGLK 778

Query: 218 GVSLPVKQSLLEEIRKINEQLIDTVVSICDKDMITSDTADANDRSEGIIVECSFVAMRIN 277
             ++    +LL+EI++ N +L++TVV ICD+D            S G IV C++  + ++
Sbjct: 779 VNNIAPGYALLQEIKETNGRLVETVVEICDED------------SLGTIVTCTYAPVALS 838

Query: 278 SNLISQQTSA----------LQLAVHPLKLLVPKGYPHCSPI-FEKLHVEACPTDCEGLS 337
           +       S           +Q  + PL+LL P  YP+ SPI  E++  +      E LS
Sbjct: 839 ATFKDHYKSGKIIFYVSKCLMQAQIQPLRLLFPMDYPYSSPIVLEEISFDTSVHKYEDLS 898

Query: 338 FKAREKFDMSVRSLPHPFSISEVARTWDVCAREVVSEYAQRNGGGSFSSKYGSWEDCLHA 388
            + R +F +S++    P     +A+TW+ CAR  + EYA+R+GGG+FSSKYG+WE  L A
Sbjct: 899 ARTRSRFSLSMKEFSEPGFSKGIAQTWNDCARATMVEYAERHGGGTFSSKYGAWETVLRA 935

BLAST of Sgr028859 vs. ExPASy Swiss-Prot
Match: F4I169 (Probable mediator of RNA polymerase II transcription subunit 15b OS=Arabidopsis thaliana OX=3702 GN=MED15B PE=3 SV=1)

HSP 1 Score: 54.3 bits (129), Expect = 3.6e-06
Identity = 46/144 (31.94%), Postives = 69/144 (47.92%), Query Frame = 0

Query: 32  LQQTDCEEVWDDYFFCSQRGNMSHQTVNDAVQQEKAFI-----ISTPGMSPSALLEEVSE 91
           L QT  ++  DD     Q  +MS Q     VQQ +  +     I TPG+S S LL E++ 
Sbjct: 112 LSQTVQDQSHDDQ-TTLQMQSMSMQGAGSRVQQIRQGVLQSLEIGTPGISASPLLPELTS 171

Query: 92  SDRE----------NEICCVAPLQRLLKMVNIMSPEALSASVSDIGSIVCMTDSVASSAP 151
            D                   P++RL++ +  +SP+ALS++V DI S+V M D +A S P
Sbjct: 172 PDGNIINPLTSTCGKSSATELPIERLIRAMKSISPQALSSAVCDIRSVVSMVDRIAGSVP 231

Query: 152 APLNKSKAAIREDLVGTVASRLRK 161
                S+A+   DLV      L++
Sbjct: 232 G--KGSRASFGVDLVAMTKCHLQE 252

BLAST of Sgr028859 vs. ExPASy TrEMBL
Match: A0A6J1CCM7 (mediator of RNA polymerase II transcription subunit 15a-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111010000 PE=4 SV=1)

HSP 1 Score: 573.2 bits (1476), Expect = 8.4e-160
Identity = 299/388 (77.06%), Postives = 320/388 (82.47%), Query Frame = 0

Query: 1   MLTGLPENGRGDELNTSLCSFFILRHLLLYFLQQTDCEE-VWDDYFFCSQRGNMSHQTVN 60
           M+ GLPENGR                         DCEE  WDD+ F SQRG +SHQ VN
Sbjct: 1   MVPGLPENGR------------------------ADCEEDAWDDFLF-SQRGKVSHQIVN 60

Query: 61  DAVQQEKAFIISTPGMSPSALLEEVSESDRENEICCVAPLQRLLKMVNIMSPEALSASVS 120
           DAVQ+EK FIISTPGMSPSALLEEVSESDR+N+IC V PLQRLLKMVN+MSP+ALSAS+S
Sbjct: 61  DAVQEEKPFIISTPGMSPSALLEEVSESDRDNDICSVTPLQRLLKMVNVMSPKALSASIS 120

Query: 121 DIGSIVCMTDSVASSAPAPLNKSKAAIREDLVGTVASRLRKGCCSRFGGTFGSRRTRHCR 180
           DI SIVCMTD VASS  APLNKSKA I EDLVGTV SRLRK  CS FGG+ GSRRTRHCR
Sbjct: 121 DIDSIVCMTDRVASS--APLNKSKAEISEDLVGTVTSRLRKR-CSIFGGSTGSRRTRHCR 180

Query: 181 SARMYASSRRLMDCESYDLNSIFTKRSRVETPEHSHWRGVSLPVKQSLLEEIRKINEQLI 240
           + RMY SSRR+M CESYDLNS FT+RSR+ETPEHSHWRG SLP KQ LLEEIRKINEQLI
Sbjct: 181 NTRMYTSSRRMMYCESYDLNSTFTERSRIETPEHSHWRGFSLPFKQYLLEEIRKINEQLI 240

Query: 241 DTVVSICDKDMITSDTADANDRSEGIIVECSFVAMRINSNLISQQTSALQLAVHPLKLLV 300
           DTVVS+CDKDMIT+D A  NDRSEGIIVECSF AMRI+SN ISQQ SALQL VHPLKLLV
Sbjct: 241 DTVVSVCDKDMITTDNAATNDRSEGIIVECSFAAMRISSNKISQQASALQLTVHPLKLLV 300

Query: 301 PKGYPHCSPIFEKLHVEACPTDCEGLSFKAREKFDMSVRSLPHPFSISEVARTWDVCARE 360
           PKGYPHCSPIF KL +EACPTDCE LS K REKF+M VRSLPHPFSISEVARTWD CARE
Sbjct: 301 PKGYPHCSPIFNKLKLEACPTDCEELSLKTREKFNMCVRSLPHPFSISEVARTWDACARE 360

Query: 361 VVSEYAQRNGGGSFSSKYGSWEDCLHAA 388
           VVSE+AQRNGGGSF+SKYGSWEDCLH A
Sbjct: 361 VVSEFAQRNGGGSFTSKYGSWEDCLHHA 360

BLAST of Sgr028859 vs. ExPASy TrEMBL
Match: A0A6J1GWK1 (mediator of RNA polymerase II transcription subunit 15a-like OS=Cucurbita moschata OX=3662 GN=LOC111458234 PE=4 SV=1)

HSP 1 Score: 461.5 bits (1186), Expect = 3.6e-126
Identity = 242/331 (73.11%), Postives = 278/331 (83.99%), Query Frame = 0

Query: 58  VNDAVQQEKAFIISTPGMSPSALLEEVSESDRENEICCVAPLQRLLKMVNIMSPEALSAS 117
           VNDA Q EKAFI+STPGMSPS+LLEEV++SDR+N+ CCV PLQRL+KMVN+MSP+ALSAS
Sbjct: 101 VNDAAQAEKAFIVSTPGMSPSSLLEEVTDSDRDNDKCCVTPLQRLIKMVNVMSPKALSAS 160

Query: 118 VSDIGSIVCMTDSVASSAPAPLNKSKAAIREDLVGTVASRLRKGCCSRFGGTFGSRRTRH 177
           VSDI SIVCMTD V+SS  APLNKSK A+  DL GTVASRLR+  CSRFGGT GSRRTRH
Sbjct: 161 VSDIDSIVCMTDRVSSS--APLNKSKGAVGMDLAGTVASRLRRK-CSRFGGTIGSRRTRH 220

Query: 178 CRSARMYASSRRLMDCESYDLNSIFTKRSR-VETPEHSHWRGVSLPVKQSLLEEIRKINE 237
            R          LMDCE +DL+S+FT+RSR +ETP+HS+W G SLPVKQSLLEEIR+INE
Sbjct: 221 YRC---------LMDCEGFDLSSLFTQRSRIIETPKHSNWHGFSLPVKQSLLEEIRRINE 280

Query: 238 QLIDTVVSICDKDMITSDTADANDRSEGIIVECSFVAMRINSNLISQQTSALQLAVHPLK 297
           QLI T VSICDKDM+T+D      RSEGI+VECSF A+   S+LIS QTSA+QLA+HPLK
Sbjct: 281 QLIYTEVSICDKDMVTTDPGATIGRSEGILVECSFGAVCTLSHLISHQTSAIQLAMHPLK 340

Query: 298 LLVPKGYPHCSPIFEKLHVEACPTDCEGLSFKAREKFDMSVRSLPHPFSISEVARTWDVC 357
           LLVP GYPH SPIF+   +EACPT CE LS +AREKF+MSVRSL HPFSISEVARTWD+C
Sbjct: 341 LLVPIGYPHISPIFK---MEACPTGCEDLSLQAREKFNMSVRSLAHPFSISEVARTWDMC 400

Query: 358 AREVVSEYAQRNGGGSFSSKYGSWEDCLHAA 388
           AR+V+SEYA++NGGGSFSSKYGSWEDCLH A
Sbjct: 401 ARDVISEYARQNGGGSFSSKYGSWEDCLHTA 416

BLAST of Sgr028859 vs. ExPASy TrEMBL
Match: A0A6J1JBE6 (mediator of RNA polymerase II transcription subunit 15a-like OS=Cucurbita maxima OX=3661 GN=LOC111482923 PE=4 SV=1)

HSP 1 Score: 457.6 bits (1176), Expect = 5.2e-125
Identity = 240/331 (72.51%), Postives = 278/331 (83.99%), Query Frame = 0

Query: 58  VNDAVQQEKAFIISTPGMSPSALLEEVSESDRENEICCVAPLQRLLKMVNIMSPEALSAS 117
           VNDA Q EKAFI+STPGMSPS+LLEEV++SDR+N+ CCV PLQRL+KMVN+MSP+ALSAS
Sbjct: 101 VNDAAQAEKAFIVSTPGMSPSSLLEEVTDSDRDNDKCCVTPLQRLIKMVNVMSPKALSAS 160

Query: 118 VSDIGSIVCMTDSVASSAPAPLNKSKAAIREDLVGTVASRLRKGCCSRFGGTFGSRRTRH 177
           VSDI SIVCMTD V+SS  APLNKSK A+  DL GTVASRLR+  CSRFGGT GSRRTRH
Sbjct: 161 VSDIDSIVCMTDRVSSS--APLNKSKGAVGMDLAGTVASRLRRK-CSRFGGTIGSRRTRH 220

Query: 178 CRSARMYASSRRLMDCESYDLNSIFTKRSR-VETPEHSHWRGVSLPVKQSLLEEIRKINE 237
            R          LMDCE +DL+S+FT+RSR +ETP+HS+W G SLPVKQSLLEEIR+INE
Sbjct: 221 YRC---------LMDCEGFDLSSLFTQRSRIIETPKHSNWHGFSLPVKQSLLEEIRRINE 280

Query: 238 QLIDTVVSICDKDMITSDTADANDRSEGIIVECSFVAMRINSNLISQQTSALQLAVHPLK 297
           QLI T VSICDKDM+T+D A    +SEGI+VECSF A+   S+LIS QTSA+QLA+ PLK
Sbjct: 281 QLIYTEVSICDKDMVTTDPAATIGKSEGILVECSFGAVCTLSHLISHQTSAIQLAMQPLK 340

Query: 298 LLVPKGYPHCSPIFEKLHVEACPTDCEGLSFKAREKFDMSVRSLPHPFSISEVARTWDVC 357
           LLVP GYPH SPIF+   +EACPT CE LS +AREKF+MSVRSL HPFSISE+ARTWD+C
Sbjct: 341 LLVPIGYPHISPIFK---MEACPTGCEDLSLQAREKFNMSVRSLAHPFSISEMARTWDMC 400

Query: 358 AREVVSEYAQRNGGGSFSSKYGSWEDCLHAA 388
           AR+V+SEYA++NGGGSFSSKYGSWEDCLH A
Sbjct: 401 ARDVISEYARQNGGGSFSSKYGSWEDCLHTA 416

BLAST of Sgr028859 vs. ExPASy TrEMBL
Match: A0A6J1CEI3 (mediator of RNA polymerase II transcription subunit 15a-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111010000 PE=4 SV=1)

HSP 1 Score: 441.4 bits (1134), Expect = 3.8e-120
Identity = 238/318 (74.84%), Postives = 256/318 (80.50%), Query Frame = 0

Query: 1   MLTGLPENGRGDELNTSLCSFFILRHLLLYFLQQTDCEE-VWDDYFFCSQRGNMSHQTVN 60
           M+ GLPENGR                         DCEE  WDD+ F SQRG +SHQ VN
Sbjct: 1   MVPGLPENGR------------------------ADCEEDAWDDFLF-SQRGKVSHQIVN 60

Query: 61  DAVQQEKAFIISTPGMSPSALLEEVSESDRENEICCVAPLQRLLKMVNIMSPEALSASVS 120
           DAVQ+EK FIISTPGMSPSALLEEVSESDR+N+IC V PLQRLLKMVN+MSP+ALSAS+S
Sbjct: 61  DAVQEEKPFIISTPGMSPSALLEEVSESDRDNDICSVTPLQRLLKMVNVMSPKALSASIS 120

Query: 121 DIGSIVCMTDSVASSAPAPLNKSKAAIREDLVGTVASRLRKGCCSRFGGTFGSRRTRHCR 180
           DI SIVCMTD VASS  APLNKSKA I EDLVGTV SRLRK  CS FGG+ GSRRTRHCR
Sbjct: 121 DIDSIVCMTDRVASS--APLNKSKAEISEDLVGTVTSRLRKR-CSIFGGSTGSRRTRHCR 180

Query: 181 SARMYASSRRLMDCESYDLNSIFTKRSRVETPEHSHWRGVSLPVKQSLLEEIRKINEQLI 240
           + RMY SSRR+M CESYDLNS FT+RSR+ETPEHSHWRG SLP KQ LLEEIRKINEQLI
Sbjct: 181 NTRMYTSSRRMMYCESYDLNSTFTERSRIETPEHSHWRGFSLPFKQYLLEEIRKINEQLI 240

Query: 241 DTVVSICDKDMITSDTADANDRSEGIIVECSFVAMRINSNLISQQTSALQLAVHPLKLLV 300
           DTVVS+CDKDMIT+D A  NDRSEGIIVECSF AMRI+SN ISQQ SALQL VHPLKLLV
Sbjct: 241 DTVVSVCDKDMITTDNAATNDRSEGIIVECSFAAMRISSNKISQQASALQLTVHPLKLLV 290

Query: 301 PKGYPHCSPIFEKLHVEA 318
           PKGYPHCSPIF KL +EA
Sbjct: 301 PKGYPHCSPIFNKLKLEA 290

BLAST of Sgr028859 vs. ExPASy TrEMBL
Match: A0A5E4ETM4 (PREDICTED: mediator of RNA OS=Prunus dulcis OX=3755 GN=ALMOND_2B013613 PE=4 SV=1)

HSP 1 Score: 254.6 bits (649), Expect = 6.6e-64
Identity = 164/360 (45.56%), Postives = 216/360 (60.00%), Query Frame = 0

Query: 48   SQRGNMSHQTVNDAVQQEKAFIISTPGMSPSALLEEVSESDRE-NEICCVA--------P 107
            S  GN+ HQ       Q ++  I TPG+S S LL E S  D   N +  ++        P
Sbjct: 1013 SNAGNVGHQQTTGVGAQVQSLAIGTPGISASPLLAEFSVPDTHVNALSTISGKSSVTEQP 1072

Query: 108  LQRLLKMVNIMSPEALSASVSDIGSIVCMTDSVASSAPAPLNKSKAAIREDLVGTVASRL 167
            L+RL+K V  MSP ALSASVSDIGS+V M D +A SAP   N S+AA+ EDLV     RL
Sbjct: 1073 LERLIKAVKSMSPNALSASVSDIGSVVSMIDRIAGSAPG--NGSRAAVGEDLVAMTKCRL 1132

Query: 168  RKGCCSRFGGTFGSRRTRHCRSA----------RMYASSRRLMDCESYDLNSIFTKRSRV 227
            +        GT G+R+ R   SA           M  S ++L + E+ DL S  T  SR+
Sbjct: 1133 QARNVMTHDGTNGTRKMRRYTSAVPLNVVSSAGSMNDSFKQLTNSETSDLES--TATSRI 1192

Query: 228  ETPEHSHWRGVSLPVKQSLLEEIRKINEQLIDTVVSICDKDMITSDTADANDRSEGIIVE 287
            + P         +    +LLEEIR+IN +LIDTVV+I D+D+    +A A +  EG IV+
Sbjct: 1193 KRPR--------IEANHALLEEIREINRRLIDTVVNISDEDV--DPSAAAAEGGEGTIVK 1252

Query: 288  CSFVAMRINSNLISQQTSALQLAVHPLKLLVPKGYPHCSPI-FEKLHVEACPTDCEGLSF 347
            CSF A+ ++ NL SQ  SA    + PL+LLVP  YP+CSPI  +K  VE    + E LS 
Sbjct: 1253 CSFDAVALSPNLKSQYASAQMSPIQPLRLLVPMNYPNCSPILLDKFPVEV-SKEYEDLSV 1312

Query: 348  KAREKFDMSVRSLPHPFSISEVARTWDVCAREVVSEYAQRNGGGSFSSKYGSWEDCLHAA 388
            KA+ KF +S+RS+  P S+ E+ARTWDVCAR V+SE+AQ++GGGSFSSKYG+WE+CL AA
Sbjct: 1313 KAKSKFSISLRSISQPMSLGEIARTWDVCARAVISEHAQQSGGGSFSSKYGTWENCLSAA 1357

BLAST of Sgr028859 vs. TAIR 10
Match: AT1G15780.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT2G10440.1); Has 103701 Blast hits to 43153 proteins in 1828 species: Archae - 30; Bacteria - 7385; Metazoa - 38639; Fungi - 11531; Plants - 7727; Viruses - 307; Other Eukaryotes - 38082 (source: NCBI BLink). )

HSP 1 Score: 187.6 bits (475), Expect = 1.9e-47
Identity = 133/363 (36.64%), Postives = 201/363 (55.37%), Query Frame = 0

Query: 51   GNMSHQTVNDAVQQEKAFIISTPGMSPSALLEEVSESDRE----------NEICCVAPLQ 110
            GN++ Q         ++  I TPG+S S LL+E +  D                   P++
Sbjct: 989  GNIARQQATGMQGVVQSLAIGTPGISASPLLQEFTSPDGNILNSSTITSGKPSATELPIE 1048

Query: 111  RLLKMVNIMSPEALSASVSDIGSIVCMTDSVASSAPAPLNKSKAAIREDLVGTVASRLR- 170
            RL++ V  +SP+ALS++VSDIGS+V M D +A SAP   N S+A++ EDLV     RL+ 
Sbjct: 1049 RLIRAVKSISPQALSSAVSDIGSVVSMVDRIAGSAPG--NGSRASVGEDLVAMTKCRLQA 1108

Query: 171  KGCCSRFGGTFGSRRTRHCRSARMYASS---------RRLMDCESYDLNSIFT---KRSR 230
            +   ++ G     +  RH  +  +  +S         ++    E+ DL S  T   K++R
Sbjct: 1109 RNFMTQEGMMATKKMKRHTTAMPLSVASLGGSVGDNYKQFAGSETSDLESTATSDGKKAR 1168

Query: 231  VETPEHSHWRGVSLPVKQSLLEEIRKINEQLIDTVVSICDKDMIT--SDTADANDRSEGI 290
             ET EH            +LLEEI++IN++LIDTVV I D +     S+ A ++   EG 
Sbjct: 1169 TET-EH------------ALLEEIKEINQRLIDTVVEISDDEDAADPSEVAISSIGCEGT 1228

Query: 291  IVECSFVAMRINSNLISQQTSALQLAVHPLKLLVPKGYPHCSP-IFEKLHVEACPTDCEG 350
             V  SF+A+ ++  L +  +S     + PL+LLVP  YP+ SP + +KL VE    + E 
Sbjct: 1229 TVRFSFIAVSLSPALKAHLSSTQMSPIQPLRLLVPCSYPNGSPSLLDKLPVETSKEN-ED 1288

Query: 351  LSFKAREKFDMSVRSLPHPFSISEVARTWDVCAREVVSEYAQRNGGGSFSSKYGSWEDCL 388
            LS KA  +F++ +RSL  P S+ ++A+TWD CAR V+ EYAQ+ GGG+FSSKYG+WE  +
Sbjct: 1289 LSSKAMARFNILLRSLSQPMSLKDIAKTWDACARAVICEYAQQFGGGTFSSKYGTWEKYV 1335

BLAST of Sgr028859 vs. TAIR 10
Match: AT2G10440.2 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G15780.1); Has 1628 Blast hits to 1350 proteins in 149 species: Archae - 0; Bacteria - 39; Metazoa - 480; Fungi - 159; Plants - 187; Viruses - 2; Other Eukaryotes - 761 (source: NCBI BLink). )

HSP 1 Score: 131.7 bits (330), Expect = 1.2e-30
Identity = 91/291 (31.27%), Postives = 148/291 (50.86%), Query Frame = 0

Query: 98  PLQRLLKMVNIMSPEALSASVSDIGSIVCMTDSVASSAPAPLNKSKAAIREDLVGTVASR 157
           P+ RL+K     SP++L+ SVS+I S++ M D +  S P+    S+A + EDL     S 
Sbjct: 579 PIDRLIKAFQAASPKSLAESVSEISSVISMVDMIGGSFPSS-GGSRAGLGEDL-----SE 638

Query: 158 LRKGCCSRFGGTFGSRRTRHCRSARMYASSRRLMDCESYDLNSIFTKRSRVETPEHSHWR 217
             +   +        R  R         SS+     +SY+   + +  S V +   S  +
Sbjct: 639 RTRNFTTHEETNLSKRMKRSINIVPPDMSSQ----IDSYE--QLSSLESEVVSTTSSGLK 698

Query: 218 GVSLPVKQSLLEEIRKINEQLIDTVVSICDKDMITSDTADANDRSEGIIVECSFVAMRIN 277
             ++    +LL+EI++ N +L++TVV ICD+D            S G IV C++  + ++
Sbjct: 699 VNNIAPGYALLQEIKETNGRLVETVVEICDED------------SLGTIVTCTYAPVALS 758

Query: 278 SNLISQQTSALQLAVHPLKLLVPKGYPHCSPI-FEKLHVEACPTDCEGLSFKAREKFDMS 337
           +       S     + PL+LL P  YP+ SPI  E++  +      E LS + R +F +S
Sbjct: 759 ATFKDHYKSGKIAQIQPLRLLFPMDYPYSSPIVLEEISFDTSVHKYEDLSARTRSRFSLS 818

Query: 338 VRSLPHPFSISEVARTWDVCAREVVSEYAQRNGGGSFSSKYGSWEDCLHAA 388
           ++    P     +A+TW+ CAR  + EYA+R+GGG+FSSKYG+WE  L A+
Sbjct: 819 MKEFSEPGFSKGIAQTWNDCARATMVEYAERHGGGTFSSKYGAWETVLRAS 845

BLAST of Sgr028859 vs. TAIR 10
Match: AT2G10440.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G15780.1); Has 8319 Blast hits to 5104 proteins in 317 species: Archae - 0; Bacteria - 285; Metazoa - 1706; Fungi - 535; Plants - 320; Viruses - 18; Other Eukaryotes - 5455 (source: NCBI BLink). )

HSP 1 Score: 128.3 bits (321), Expect = 1.4e-29
Identity = 92/301 (30.56%), Postives = 150/301 (49.83%), Query Frame = 0

Query: 98  PLQRLLKMVNIMSPEALSASVSDIGSIVCMTDSVASSAPAPLNKSKAAIREDLVGTVASR 157
           P+ RL+K     SP++L+ SVS+I S++ M D +  S P+    S+A + EDL     S 
Sbjct: 659 PIDRLIKAFQAASPKSLAESVSEISSVISMVDMIGGSFPSS-GGSRAGLGEDL-----SE 718

Query: 158 LRKGCCSRFGGTFGSRRTRHCRSARMYASSRRLMDCESYDLNSIFTKRSRVETPEHSHWR 217
             +   +        R  R         SS+     +SY+   + +  S V +   S  +
Sbjct: 719 RTRNFTTHEETNLSKRMKRSINIVPPDMSSQ----IDSYE--QLSSLESEVVSTTSSGLK 778

Query: 218 GVSLPVKQSLLEEIRKINEQLIDTVVSICDKDMITSDTADANDRSEGIIVECSFVAMRIN 277
             ++    +LL+EI++ N +L++TVV ICD+D            S G IV C++  + ++
Sbjct: 779 VNNIAPGYALLQEIKETNGRLVETVVEICDED------------SLGTIVTCTYAPVALS 838

Query: 278 SNLISQQTSA----------LQLAVHPLKLLVPKGYPHCSPI-FEKLHVEACPTDCEGLS 337
           +       S           +Q  + PL+LL P  YP+ SPI  E++  +      E LS
Sbjct: 839 ATFKDHYKSGKIIFYVSKCLMQAQIQPLRLLFPMDYPYSSPIVLEEISFDTSVHKYEDLS 898

Query: 338 FKAREKFDMSVRSLPHPFSISEVARTWDVCAREVVSEYAQRNGGGSFSSKYGSWEDCLHA 388
            + R +F +S++    P     +A+TW+ CAR  + EYA+R+GGG+FSSKYG+WE  L A
Sbjct: 899 ARTRSRFSLSMKEFSEPGFSKGIAQTWNDCARATMVEYAERHGGGTFSSKYGAWETVLRA 935

BLAST of Sgr028859 vs. TAIR 10
Match: AT1G15770.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G15780.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 54.3 bits (129), Expect = 2.5e-07
Identity = 46/144 (31.94%), Postives = 69/144 (47.92%), Query Frame = 0

Query: 32  LQQTDCEEVWDDYFFCSQRGNMSHQTVNDAVQQEKAFI-----ISTPGMSPSALLEEVSE 91
           L QT  ++  DD     Q  +MS Q     VQQ +  +     I TPG+S S LL E++ 
Sbjct: 112 LSQTVQDQSHDDQ-TTLQMQSMSMQGAGSRVQQIRQGVLQSLEIGTPGISASPLLPELTS 171

Query: 92  SDRE----------NEICCVAPLQRLLKMVNIMSPEALSASVSDIGSIVCMTDSVASSAP 151
            D                   P++RL++ +  +SP+ALS++V DI S+V M D +A S P
Sbjct: 172 PDGNIINPLTSTCGKSSATELPIERLIRAMKSISPQALSSAVCDIRSVVSMVDRIAGSVP 231

Query: 152 APLNKSKAAIREDLVGTVASRLRK 161
                S+A+   DLV      L++
Sbjct: 232 G--KGSRASFGVDLVAMTKCHLQE 252