Sgr027668 (gene) Monk fruit (Qingpiguo) v1

BLAST of Sgr027668 vs. NCBI nr
Match: XP_022156753.1 (cytochrome P450 CYP82D47-like [Momordica charantia])

HSP 1 Score: 798.9 bits (2062), Expect = 2.6e-227
Identity = 400/534 (74.91%), Postives = 453/534 (84.83%), Query Frame = 0

Query: 5   TTSSMEIILPYLN-SSIAGLFLLLLFSFFVLKK-ARACKRRQPPEAAGGWPIIGHLRLLG 64
           T +SMEII PYLN +SI  LFLLLL S+ VLKK ++A KRR PP A G WPIIGHL LL 
Sbjct: 6   TATSMEIISPYLNTNSIIPLFLLLLISYLVLKKLSKASKRRAPPAAGGAWPIIGHLPLLS 65

Query: 65  GSQLPHETLGAMADKYGPIFSIRVGVHPSLVISSWEVAKECYTTLDSVVSSRPKSLGGKL 124
           G QLPHETLGAMADKYGPIF+I+VGVHP+LVISSWEVAKECYTT DS+VSSRPK++GGKL
Sbjct: 66  GPQLPHETLGAMADKYGPIFTIQVGVHPTLVISSWEVAKECYTTHDSIVSSRPKTVGGKL 125

Query: 125 LGYNFAAFGFRPYDSFYRSIRKTIASEVLSNRRLEL--QRHIRVSEVKRSVKELYNLWTQ 184
           LGYNFA FGFRPYDSFYRSIR+ IASE+LSNRRLEL  QR IR SEVKRSV+ELY+LW +
Sbjct: 126 LGYNFAGFGFRPYDSFYRSIRRIIASEMLSNRRLELQFQRDIRDSEVKRSVRELYDLWEK 185

Query: 185 REEGSDH----ILIDADQWIGNINLNVILMMVCGKRFLGGSASDEKEMRQCRKVMRDFFD 244
           + E SD     + +D D W+G+++L VILMMVCGKRF+G     EKEM++CR+ MR+FFD
Sbjct: 186 KRETSDDDDVAVNVDLDGWVGSVDLKVILMMVCGKRFVG-----EKEMKRCRRAMREFFD 245

Query: 245 LTGQFTVGDAIPFLRWLDLGGYAKAMKKTAKEMDCLVEEWLEEHRRRKRDSGATDGERDF 304
           L GQF VGDA+PFLRWLDLGGY KAMKKTAKE+D ++EEWL+EHRR+     A DG  DF
Sbjct: 246 LAGQFMVGDALPFLRWLDLGGYQKAMKKTAKELDSIMEEWLDEHRRKADSGAAADGGPDF 305

Query: 305 MDVMLSILEEMDLAGYDADT-------SIISGGTDTITVTLTWAISLLLNNREALRRVQE 364
           MDVMLS LE +DLAGYDA T       +II+GGTDT T T+ WAISLLLNNREAL+R QE
Sbjct: 306 MDVMLSTLEGIDLAGYDAATVNKATCMTIIAGGTDTTTETILWAISLLLNNREALKRAQE 365

Query: 365 ELDIHVGNKRLVDESDLSKLVYLQAVVKETLRLYPAGPLSGVREFSRDCTVGGYDVAAGT 424
           ELDIHVGNKRL+DESD+SKL YLQAVVKETLRLYPAGPLSG+REFSRDCTVGGYDVA GT
Sbjct: 366 ELDIHVGNKRLLDESDISKLEYLQAVVKETLRLYPAGPLSGIREFSRDCTVGGYDVAVGT 425

Query: 425 RLITNLWKIQTDPRVWPEPLEFRPERFLSSHQQLDVKGQNFELAPFGCGRRVCPGAGLGV 484
           RLITNLWKIQTDPRVW EPL+F+PERFLSSH+ LDVKGQNFE  PFGCGRRVCPG G G+
Sbjct: 426 RLITNLWKIQTDPRVWAEPLKFKPERFLSSHKHLDVKGQNFEFTPFGCGRRVCPGLGFGI 485

Query: 485 QMTQLVLASLIHSVELGTRSDDLVDMAANFGLTIHRATPLQVLVKPRILASAYA 524
           QMTQLVLASLIHS+EL TR D+ VDM A+FG+T+HR  PLQVL+KPR+LASAYA
Sbjct: 486 QMTQLVLASLIHSMELKTRFDEPVDMTADFGITMHRVNPLQVLIKPRLLASAYA 534

BLAST of Sgr027668 vs. NCBI nr
Match: XP_022976621.1 (cytochrome P450 CYP82D47-like [Cucurbita maxima])

HSP 1 Score: 786.2 bits (2029), Expect = 1.8e-223
Identity = 392/527 (74.38%), Postives = 447/527 (84.82%), Query Frame = 0

Query: 4   LTTSSMEIILPYLNSSIAGLFLLLLFSFFVLKKARACKRRQPPEAAGGWPIIGHLRLL-G 63
           LT  S+ II PYLN+ +A LFL LL SFFV KKA A KRR+PPE AGGWPIIGHL LL  
Sbjct: 3   LTGISIGIIPPYLNTFVAALFLFLLSSFFVFKKATAPKRREPPEVAGGWPIIGHLPLLKA 62

Query: 64  GSQLPHETLGAMADKYGPIFSIRVGVHPSLVISSWEVAKECYTTLDSVVSSRPKSLGGKL 123
            SQLPH+TLGA+ADKYGPIF IRVG  P+L+ISS E+A+EC++TLDS+V SRPKS+GGKL
Sbjct: 63  DSQLPHQTLGALADKYGPIFRIRVGAQPTLIISSSELARECHSTLDSIVCSRPKSVGGKL 122

Query: 124 LGYNFAAFGFRPYDSFYRSIRKTIASEVLSNRRLELQRHIRVSEVKRSVKELYNLWTQRE 183
           LGYN+AAFG RPYDSFYRS+RK + SEVLSNR L+L+R IRVSEVK+S+KELY+ WT RE
Sbjct: 123 LGYNYAAFGARPYDSFYRSMRKIVVSEVLSNRCLDLRRDIRVSEVKKSLKELYDQWTNRE 182

Query: 184 EGSDHILIDADQWIGNINLNVILMMVCGKRFLGGSASDEKEMRQCRKVMRDFFDLTGQFT 243
           EGSDHIL+D +QWIGNINL V+LMMVCGKRFLGGSA DE EM++CRK+MRDF DL G+F 
Sbjct: 183 EGSDHILVDIEQWIGNINLKVVLMMVCGKRFLGGSADDE-EMKRCRKIMRDFLDLMGKFL 242

Query: 244 VGDAIPFLRWLDLGGYAKAMKKTAKEMDCLVEEWLEEHRRRKRDSGATDGERDFMDVMLS 303
           VGD+IPFLRWLDLGGY KAMK ++K++D L+EEWLEEHRR++ D        D MDVMLS
Sbjct: 243 VGDSIPFLRWLDLGGYEKAMKISSKQLDSLLEEWLEEHRRKRTD------HNDLMDVMLS 302

Query: 304 ILEEMDLAGYDADT-------SIISGGTDTITVTLTWAISLLLNNREALRRVQEELDIHV 363
            LE MDLAGYDADT       SII+GGTDT+TVTL+WAISLLLNNREALR+ QEELDIHV
Sbjct: 303 NLEAMDLAGYDADTVNKATCLSIITGGTDTVTVTLSWAISLLLNNREALRKTQEELDIHV 362

Query: 364 GNKRLVDESDLSKLVYLQAVVKETLRLYPAGPLSGVREFSRDCTVGGYDVAAGTRLITNL 423
           G  RLVDESD+SKLVYLQAVV ETLRLYP GPLSGVR FS DCTVGGYDV AGT LIT+L
Sbjct: 363 GKDRLVDESDISKLVYLQAVVNETLRLYPPGPLSGVRVFSEDCTVGGYDVVAGTHLITDL 422

Query: 424 WKIQTDPRVWPEPLEFRPERFLSSHQQLDVKGQNFELAPFGCGRRVCPGAGLGVQMTQLV 483
           WKIQT+P+VW EPLEF+PERF+S  +QLDVKGQNFE APFG GRR CPG  LG+QMTQLV
Sbjct: 423 WKIQTNPQVWAEPLEFKPERFVSGEKQLDVKGQNFEFAPFGLGRRACPGRSLGIQMTQLV 482

Query: 484 LASLIHSVELGTRSDDLVDMAANFGLTIHRATPLQVLVKPRILASAY 523
           LASLIHS+ELGT SD+ VDM A FGLT++RA PLQ+LVKPR+LA AY
Sbjct: 483 LASLIHSLELGTGSDEPVDMTAGFGLTMYRAKPLQILVKPRLLACAY 522

BLAST of Sgr027668 vs. NCBI nr
Match: XP_023535458.1 (cytochrome P450 CYP82D47-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 780.8 bits (2015), Expect = 7.4e-222
Identity = 389/523 (74.38%), Postives = 443/523 (84.70%), Query Frame = 0

Query: 8   SMEIILPYLNSSIAGLFLLLLFSFFVLKKARACKRRQPPEAAGGWPIIGHLRLL-GGSQL 67
           S+ II PYLN+ +A LFL LL SFFV KKA A KRR+PPE AGGWPIIGHL LL   SQL
Sbjct: 7   SIGIIPPYLNTFVAALFLFLLSSFFVFKKATAPKRREPPEVAGGWPIIGHLPLLKADSQL 66

Query: 68  PHETLGAMADKYGPIFSIRVGVHPSLVISSWEVAKECYTTLDSVVSSRPKSLGGKLLGYN 127
           PH+TLGA+ADKYGP+F IRVG  P+L+ISS E+A+EC++TLDS+V SRPKS+GGKLLGYN
Sbjct: 67  PHQTLGALADKYGPVFRIRVGAQPTLIISSSELARECHSTLDSIVCSRPKSVGGKLLGYN 126

Query: 128 FAAFGFRPYDSFYRSIRKTIASEVLSNRRLELQRHIRVSEVKRSVKELYNLWTQREEGSD 187
           +AAFG RPYDSFYRS+RK + SEVLSNR L L+R IRVSEV +S+KELYN WT+REEGSD
Sbjct: 127 YAAFGARPYDSFYRSMRKIVVSEVLSNRCLGLRRDIRVSEVNKSLKELYNQWTKREEGSD 186

Query: 188 HILIDADQWIGNINLNVILMMVCGKRFLGGSASDEKEMRQCRKVMRDFFDLTGQFTVGDA 247
           HIL+D +QWIGN+NL V+LMMVCGKRFLG SA DE EM++CRKVMR+F DL G+F VGD+
Sbjct: 187 HILVDIEQWIGNVNLRVVLMMVCGKRFLGDSADDE-EMKRCRKVMREFLDLMGKFLVGDS 246

Query: 248 IPFLRWLDLGGYAKAMKKTAKEMDCLVEEWLEEHRRRKRDSGATDGERDFMDVMLSILEE 307
           IPFLRWLDLGGY KAMK T+K++D L+EEWLEEHRR++ D        D MDVMLS LE 
Sbjct: 247 IPFLRWLDLGGYEKAMKITSKQLDSLLEEWLEEHRRKRTD------HNDLMDVMLSNLEG 306

Query: 308 MDLAGYDADT-------SIISGGTDTITVTLTWAISLLLNNREALRRVQEELDIHVGNKR 367
           MDLAGYDADT       SII+GG+DT+TVTL WAISLLLNNREALR+ QEELDIHVG  R
Sbjct: 307 MDLAGYDADTVNKATCLSIITGGSDTVTVTLAWAISLLLNNREALRKTQEELDIHVGKDR 366

Query: 368 LVDESDLSKLVYLQAVVKETLRLYPAGPLSGVREFSRDCTVGGYDVAAGTRLITNLWKIQ 427
           LVDESD+SKLVYLQAVV ETLRLYP GPLSGVR FS DCTVGGYDV AGT LITNLWKIQ
Sbjct: 367 LVDESDISKLVYLQAVVNETLRLYPPGPLSGVRVFSEDCTVGGYDVVAGTHLITNLWKIQ 426

Query: 428 TDPRVWPEPLEFRPERFLSSHQQLDVKGQNFELAPFGCGRRVCPGAGLGVQMTQLVLASL 487
           T+P+VW EPLEF+PERF+S  +QLDVKGQNFE APFG GRR CPG  LG+QMTQLVLASL
Sbjct: 427 TNPQVWAEPLEFKPERFVSGEKQLDVKGQNFEFAPFGLGRRACPGMSLGIQMTQLVLASL 486

Query: 488 IHSVELGTRSDDLVDMAANFGLTIHRATPLQVLVKPRILASAY 523
           IHS+ELGT SD+ VDMAA FGLT++RA PLQ+LVKPR+LA AY
Sbjct: 487 IHSLELGTGSDEPVDMAAGFGLTMYRANPLQLLVKPRLLACAY 522

BLAST of Sgr027668 vs. NCBI nr
Match: XP_022937188.1 (cytochrome P450 CYP82D47-like [Cucurbita moschata])

HSP 1 Score: 780.8 bits (2015), Expect = 7.4e-222
Identity = 390/523 (74.57%), Postives = 442/523 (84.51%), Query Frame = 0

Query: 8   SMEIILPYLNSSIAGLFLLLLFSFFVLKKARACKRRQPPEAAGGWPIIGHLRLL-GGSQL 67
           S+ II PYLN+ +A LFL LL SFFV KKA A KRR+PPE AGGWPIIGHL LL   SQL
Sbjct: 7   SIGIIPPYLNTFVAALFLFLLSSFFVFKKATAPKRREPPEVAGGWPIIGHLPLLKADSQL 66

Query: 68  PHETLGAMADKYGPIFSIRVGVHPSLVISSWEVAKECYTTLDSVVSSRPKSLGGKLLGYN 127
           PH+TLGA+ADKYGPIF IRVG  P+L+ISS E+A+EC++TLDS+V SRPKS+GGKLLGYN
Sbjct: 67  PHQTLGALADKYGPIFRIRVGAQPTLIISSSELARECHSTLDSIVCSRPKSVGGKLLGYN 126

Query: 128 FAAFGFRPYDSFYRSIRKTIASEVLSNRRLELQRHIRVSEVKRSVKELYNLWTQREEGSD 187
           +AAFG RPYDSFYRS+RK + SEVLSNR L L+R IRVSEVK+S+KELYN WT+REEGSD
Sbjct: 127 YAAFGARPYDSFYRSMRKIVVSEVLSNRCLGLRRDIRVSEVKKSLKELYNQWTKREEGSD 186

Query: 188 HILIDADQWIGNINLNVILMMVCGKRFLGGSASDEKEMRQCRKVMRDFFDLTGQFTVGDA 247
           HIL+D +QWIGN+NL V+LMMVCGKRFLG SA DE EM++CRKVMR+F DL G+F VGD+
Sbjct: 187 HILVDIEQWIGNVNLRVVLMMVCGKRFLGDSADDE-EMKRCRKVMREFLDLMGKFLVGDS 246

Query: 248 IPFLRWLDLGGYAKAMKKTAKEMDCLVEEWLEEHRRRKRDSGATDGERDFMDVMLSILEE 307
           IPFLRWLDLGGY KAMK T+K++D L+EEWLEEHRR++ D        D MDVMLS LE 
Sbjct: 247 IPFLRWLDLGGYEKAMKITSKQLDSLLEEWLEEHRRKRTD------HNDLMDVMLSNLEG 306

Query: 308 MDLAGYDADT-------SIISGGTDTITVTLTWAISLLLNNREALRRVQEELDIHVGNKR 367
           MDLAGYDADT       SII+GGTDT+TVTL+WAISLLLNNREALR+ QEELDIHVG  R
Sbjct: 307 MDLAGYDADTVNKATCLSIITGGTDTVTVTLSWAISLLLNNREALRKTQEELDIHVGKDR 366

Query: 368 LVDESDLSKLVYLQAVVKETLRLYPAGPLSGVREFSRDCTVGGYDVAAGTRLITNLWKIQ 427
            VDESD+SKLVYLQAVV ETLRLYP GPLSGVR FS DCTVGGYDV AGT LITNLWKIQ
Sbjct: 367 QVDESDISKLVYLQAVVNETLRLYPPGPLSGVRVFSEDCTVGGYDVVAGTHLITNLWKIQ 426

Query: 428 TDPRVWPEPLEFRPERFLSSHQQLDVKGQNFELAPFGCGRRVCPGAGLGVQMTQLVLASL 487
           T+P+VW EPLEF+PERF+S  +QLDVKGQNFE APFG GRR CPG  LG+QMTQLVLASL
Sbjct: 427 TNPQVWAEPLEFKPERFVSGEKQLDVKGQNFEFAPFGLGRRACPGMSLGIQMTQLVLASL 486

Query: 488 IHSVELGTRSDDLVDMAANFGLTIHRATPLQVLVKPRILASAY 523
           IHS+ELGT SD+ VDMAA FGLT++RA PLQVLVKPR+   AY
Sbjct: 487 IHSLELGTGSDEPVDMAAGFGLTMYRANPLQVLVKPRLFTCAY 522

BLAST of Sgr027668 vs. NCBI nr
Match: XP_022937187.1 (cytochrome P450 CYP82D47-like [Cucurbita moschata])

HSP 1 Score: 768.1 bits (1982), Expect = 5.0e-218
Identity = 384/532 (72.18%), Postives = 443/532 (83.27%), Query Frame = 0

Query: 5   TTSSMEIILPYLNSSIAG-LFLLLLFSFFVLKKARACKRR-QPPEAAGGWPIIGHLRLLG 64
           TTS  + I PYLNS++AG LFLLLLFS+FVLKKA + KRR QPPE AGGWPIIGHLRLL 
Sbjct: 13  TTSMADQISPYLNSTVAGLLFLLLLFSYFVLKKATSRKRRSQPPEVAGGWPIIGHLRLLS 72

Query: 65  -GSQLPHETLGAMADKYGPIFSIRVGVHPSLVISSWEVAKECYTTLDSVVSSRPKSLGGK 124
             SQL HETLG +ADKYGPIF IR+GV P+LVISSWEVA ECYTTLDS+VSSRPK +  K
Sbjct: 73  TDSQLLHETLGGLADKYGPIFGIRIGVRPALVISSWEVATECYTTLDSIVSSRPKIILQK 132

Query: 125 LLGYNFAAFGFRPY-DSFYRSIRKTIASEVLSNRRLELQRHIRVSEVKRSVKELYNLWTQ 184
            LGYNFA FGFR + DSFYR++RK +ASE LSNRRLELQR IRVSEVKRSVKE+Y+LW +
Sbjct: 133 KLGYNFAGFGFRHHCDSFYRNMRKIVASEALSNRRLELQRDIRVSEVKRSVKEVYSLWAK 192

Query: 185 REEGS--DHILIDADQWIGNINLNVILMMVCGKRFLGGSASDEKEMRQCRKVMRDFFDLT 244
             E S   ++++D D+WIGNI LNVILMMVCGKRF+GGS  DE+EM +CRK MR FFDL 
Sbjct: 193 TGEASSDSYLVLDLDEWIGNIALNVILMMVCGKRFVGGSCGDEREMERCRKAMRGFFDLG 252

Query: 245 GQFTVGDAIPFLRWLDLGGYAKAMKKTAKEMDCLVEEWLEEHRRRKRDSGATDGERDFMD 304
           G+F VGDAIPFL+WLDLGGY KA  K  KE+DCL+EEWLEEHRR+KRD    DGE D M 
Sbjct: 253 GKFIVGDAIPFLKWLDLGGYQKATNKVWKELDCLMEEWLEEHRRKKRDCSDADGEHDLMA 312

Query: 305 VMLSILEEMDLAGYDADT-------SIISGGTDTITVTLTWAISLLLNNREALRRVQEEL 364
           VM S+LE +DLAGYDADT       ++ISGGTDT+ VTLTWAISLLLNN+EALR+V+EEL
Sbjct: 313 VMPSLLEGLDLAGYDADTVNKATCLTLISGGTDTMAVTLTWAISLLLNNQEALRKVEEEL 372

Query: 365 DIHVGNKRLVDESDLSKLVYLQAVVKETLRLYPAGPLSGVREFSRDCTVGGYDVAAGTRL 424
           DIHVG +R VDESD+SKLVYLQAV+KETLRLYPAGPLSG RE S+DCT+GGY+VAAGT L
Sbjct: 373 DIHVGKERAVDESDISKLVYLQAVIKETLRLYPAGPLSGAREVSQDCTIGGYNVAAGTYL 432

Query: 425 ITNLWKIQTDPRVWPEPLEFRPERFLSSHQQLDVKGQNFELAPFGCGRRVCPGAGLGVQM 484
           ITN+WKIQ DPR+W EP EF+PERFLSSH+ +DVKGQ+FEL+PFG GRR CPG G+ + M
Sbjct: 433 ITNIWKIQRDPRIWQEPSEFKPERFLSSHKHMDVKGQHFELSPFGYGRRACPGQGISLLM 492

Query: 485 TQLVLASLIHSVELGTRSDDLVDMAANFGLTIHRATPLQVLVKPRILASAYA 524
           T LVLASL+HS +L TR D+ VDM AN GLT+HR  PLQVLVKPR+L +AYA
Sbjct: 493 TPLVLASLVHSFQLRTRRDEAVDMTANLGLTMHRVNPLQVLVKPRLLPTAYA 544

BLAST of Sgr027668 vs. ExPASy Swiss-Prot
Match: O49859 (Cytochrome P450 82A4 OS=Glycine max OX=3847 GN=CYP82A4 PE=2 SV=1)

HSP 1 Score: 529.6 bits (1363), Expect = 3.9e-149
Identity = 265/525 (50.48%), Postives = 357/525 (68.00%), Query Frame = 0

Query: 9   MEIILPYLNSSIAG----LFLLLLFSFFVLKKARACKRRQPPEAAGGWPIIGHLRLLGGS 68
           ME++L +LN++  G    +FLL LF +  LKK      ++ P   G WPI GHL LL GS
Sbjct: 1   MELVLHFLNTTTIGVVSLIFLLCLFLYGPLKKVHG-SSKEAPTVGGAWPIFGHLPLLIGS 60

Query: 69  QLPHETLGAMADKYGPIFSIRVGVHPSLVISSWEVAKECYTTLDSVVSSRPKSLGGKLLG 128
           + PH+ LGA+A+K+GP+F+I++G   +LV+S WE+A+EC+TT D  VS+RPK L  +L+ 
Sbjct: 61  KSPHKALGALAEKHGPLFTIKLGAKKALVVSDWEMARECFTTNDVAVSARPKLLVAELMC 120

Query: 129 YNFAAFGFRPYDSFYRSIRKTIASEVLSNRRLELQRHIRVSEVKRSVKELYNLWTQREEG 188
           YN A     PY  ++R +RK I +E+LS+ R+E  + +RVSEV+ S+ ELY++W  ++  
Sbjct: 121 YNNAMLLVAPYGPYWRELRKIIVTEILSSSRVEQLQDVRVSEVQNSIVELYDVWRSQKNE 180

Query: 189 SDHILIDADQWIGNINLNVILMMVCGKRFLGGSASDEKEMRQCRKVMRDFFDLTGQFTVG 248
           SD+  ++  QW      N++L MV GKRFL  +A+DEK   +C K + +F  L G FTVG
Sbjct: 181 SDYASVELKQWFAQPIFNMVLRMVVGKRFLSATATDEK-AEKCVKAVDEFMRLAGVFTVG 240

Query: 249 DAIPFLRWLDLGGYAKAMKKTAKEMDCLVEEWLEEHRRRKRDSGATDGERDFMDVMLSIL 308
           DAIP+LRWLD GGY KAMK+TAKE+D ++ EWLEEHR+++      DG +DFM+VMLS L
Sbjct: 241 DAIPYLRWLDFGGYEKAMKETAKELDVMISEWLEEHRQKRALGEGVDGAQDFMNVMLSSL 300

Query: 309 EEMDLAGYDADT-------SIISGGTDTITVTLTWAISLLLNNREALRRVQEELDIHVGN 368
           +   + G DADT       +II  GT+    T+ WA+ L+L N   L   + ELDI VG 
Sbjct: 301 DGKTIDGIDADTLIKSTVLTIIQAGTEASISTIIWAMCLILKNPLILEN-KAELDIQVGK 360

Query: 369 KRLVDESDLSKLVYLQAVVKETLRLYPAGPLSGVREFSRDCTVGGYDVAAGTRLITNLWK 428
            R + ESD+S LVYLQAVVKETLRLY  GPLS  REF+ DCT+GGY V  GTRLITN+WK
Sbjct: 361 DRCICESDISNLVYLQAVVKETLRLYAPGPLSSPREFAEDCTLGGYHVKKGTRLITNIWK 420

Query: 429 IQTDPRVWPEPLEFRPERFLSSHQQLDVKGQNFELAPFGCGRRVCPGAGLGVQMTQLVLA 488
           I TDP VW +P EF+P+RFL++H+ +DVKG +F+L PFG GRRVCPG   G+Q   L LA
Sbjct: 421 IHTDPNVWSDPFEFKPDRFLTTHKDIDVKGHHFQLLPFGSGRRVCPGISFGLQTVHLALA 480

Query: 489 SLIHSVELGTRSDDLVDMAANFGLTIHRATPLQVLVKPRILASAY 523
           S +HS E+   S + +DM   FG+T  +ATPL+VLVKP +  S Y
Sbjct: 481 SFLHSFEILNPSTEPLDMTEAFGVTNTKATPLEVLVKPCLSPSCY 522

BLAST of Sgr027668 vs. ExPASy Swiss-Prot
Match: Q9SZ46 (Xanthotoxin 5-hydroxylase CYP82C4 OS=Arabidopsis thaliana OX=3702 GN=CYP82C4 PE=1 SV=1)

HSP 1 Score: 525.8 bits (1353), Expect = 5.6e-148
Identity = 265/519 (51.06%), Postives = 364/519 (70.13%), Query Frame = 0

Query: 16  LNSSIAGLFL-LLLFSFFVL-KKARACKRRQPPEAAGGWPIIGHLRLLGG-SQLPHETLG 75
           +++S+  LF+ +L+F F  L KK++  K  + P  +G WPIIGHL LLGG  QL + TLG
Sbjct: 1   MDTSLFSLFVPILVFVFIALFKKSKKPKYVKAPAPSGAWPIIGHLHLLGGKEQLLYRTLG 60

Query: 76  AMADKYGPIFSIRVGVHPSLVISSWEVAKECYTTLDSVVSSRPKSLGGKLLGYNFAAFGF 135
            MAD YGP  S+++G + + V+SS+EVAK+C+T  D  ++SRP +   K +GYNFA FGF
Sbjct: 61  KMADHYGPAMSLQLGSNEAFVVSSFEVAKDCFTVNDKALASRPMTAAAKHMGYNFAVFGF 120

Query: 136 RPYDSFYRSIRKTIASEVLSNRRLELQRHIRVSEVKRSVKELYNLWTQREEGSDHILIDA 195
            PY +F+R +RK    E+LSNRRL++ +H+RVSE+   VK+LY+LW  +  G+  +++D 
Sbjct: 121 APYSAFWREMRKIATIELLSNRRLQMLKHVRVSEITMGVKDLYSLWF-KNGGTKPVMVDL 180

Query: 196 DQWIGNINLNVILMMVCGKRFLGG----SASDEKEMRQCRKVMRDFFDLTGQFTVGDAIP 255
             W+ ++ LN+I+ MV GKR+ GG    S+ D +E  QC+K +  FF L G FTV DA P
Sbjct: 181 KSWLEDMTLNMIVRMVAGKRYFGGGGSVSSEDTEEAMQCKKAIAKFFHLIGIFTVSDAFP 240

Query: 256 FLRWLDLGGYAKAMKKTAKEMDCLVEEWLEEHRRRKRDSGATDGERDFMDVMLSILEEMD 315
            L + DL G+ K MK+T  E+D ++E W+E HR++++ SG  + + DF+DVM+S+ E+  
Sbjct: 241 TLSFFDLQGHEKEMKQTGSELDVILERWIENHRQQRKFSGTKENDSDFIDVMMSLAEQGK 300

Query: 316 LA--GYDADTSI-------ISGGTDTITVTLTWAISLLLNNREALRRVQEELDIHVGNKR 375
           L+   YDA+TSI       I GG+DT   TLTWAISLLLNN+E L++ Q+E+DIHVG  R
Sbjct: 301 LSHLQYDANTSIKSTCLALILGGSDTSASTLTWAISLLLNNKEMLKKAQDEIDIHVGRDR 360

Query: 376 LVDESDLSKLVYLQAVVKETLRLYPAGPLSGVREFSRDCTVGGYDVAAGTRLITNLWKIQ 435
            V++SD+  LVYLQA++KETLRLYPAGPL G RE   DCTV GY V  GTRLI N+WKIQ
Sbjct: 361 NVEDSDIENLVYLQAIIKETLRLYPAGPLLGPREAMEDCTVAGYYVPCGTRLIVNVWKIQ 420

Query: 436 TDPRVWPEPLEFRPERFLSSH-QQLDVKGQNFELAPFGCGRRVCPGAGLGVQMTQLVLAS 495
            DP+V+ EP EFRPERF++   ++ DV+GQNFEL PFG GRR CPG+ L +Q+  L LA 
Sbjct: 421 RDPKVYMEPNEFRPERFITGEAKEFDVRGQNFELMPFGSGRRSCPGSSLAMQVLHLGLAR 480

Query: 496 LIHSVELGTRSDDLVDMAANFGLTIHRATPLQVLVKPRI 518
            +HS ++ T  D  VDM+ N GLTI +ATPL+VL+ PRI
Sbjct: 481 FLHSFDVKTVMDMPVDMSENPGLTIPKATPLEVLISPRI 518

BLAST of Sgr027668 vs. ExPASy Swiss-Prot
Match: Q43068 (Cytochrome P450 82A1 (Fragment) OS=Pisum sativum OX=3888 GN=CYP82A1 PE=2 SV=2)

HSP 1 Score: 524.6 bits (1350), Expect = 1.3e-147
Identity = 252/541 (46.58%), Postives = 360/541 (66.54%), Query Frame = 0

Query: 12  ILPYLNSSIAGLFLLLLFSFFVLKKARACKRRQPPEAAGGWPIIGHLRLLGGSQLPHETL 71
           +L YLN++      L+   FF+ + ++    ++PP  +G WP++GHL L+  +Q PH+TL
Sbjct: 2   VLNYLNTTTIAFISLISLLFFLFRFSKVSHTKEPPIISGSWPLLGHLPLMRNTQTPHKTL 61

Query: 72  GAMADKYGPIFSIRVGVHPSLVISSWEVAKECYTTLDSVVSSRPKSLGGKLLGYNFAAFG 131
           GA+ DKYGPIF+I++G   +LV+S+WE+AKEC+T  D VVSSRPK +  +L+ YN A  G
Sbjct: 62  GALVDKYGPIFTIKLGATNALVLSNWELAKECFTKNDIVVSSRPKPVAVELMSYNQAFIG 121

Query: 132 FRPYDSFYRSIRKTIASEVLSNRRLELQRHIRVSEVKRSVKELYNLWTQR---------- 191
           + PY +++R +RK +  E+LSNRR+EL  HIRVSEV+ S+KEL N+W+ +          
Sbjct: 122 WAPYGAYWRQLRKIVTLEILSNRRIELLSHIRVSEVQTSIKELVNVWSNQISSQYGLLDD 181

Query: 192 ---------EEGSDHILIDADQWIGNINLNVILMMVCGKRFLGG-SASDEKEMRQCRKVM 251
                       +D++ ++  +W   + LN++L MV GKR  G     +++E ++  + +
Sbjct: 182 TKSSSTNDEPSTTDYVSVELKKWFAQLTLNMVLRMVVGKRCFGDVDVENKEEAKRFLENI 241

Query: 252 RDFFDLTGQFTVGDAIPFLRWLDLGGYAKAMKKTAKEMDCLVEEWLEEHRRRKRDSGATD 311
           RDF  L G FTVGD +PFL+WLDLGG+ K MKK AK+ D ++ EWLEEH R K+  G+ D
Sbjct: 242 RDFMRLIGTFTVGDGVPFLKWLDLGGHEKEMKKCAKKFDVMLNEWLEEH-REKKGLGSED 301

Query: 312 ---GERDFMDVMLSILEEMDLAGYDADT-------SIISGGTDTITVTLTWAISLLLNNR 371
              GERDFMD ML +L++  + G+D DT        +I GG+DT   TLTWA+ LLL + 
Sbjct: 302 KVVGERDFMDAMLLVLKDKPIEGFDVDTIIKATTLELILGGSDTTAGTLTWAMCLLLKHP 361

Query: 372 EALRRVQEELDIHVGNKRLVDESDLSKLVYLQAVVKETLRLYPAGPLSGVREFSRDCTVG 431
             L +++EEL+ ++G +R V+ESD++KLVYL A++KETLRLYP  P S  REF+ DCT+G
Sbjct: 362 HVLEKLKEELNTYIGKERCVNESDINKLVYLHAIIKETLRLYPPAPFSSPREFTEDCTIG 421

Query: 432 GYDVAAGTRLITNLWKIQTDPRVWPEPLEFRPERFLSSHQQLDVKGQNFELAPFGCGRRV 491
           GY +  GTRL+ NLWKI  DP VWP+PLEF+PERFLS+H+ +DV+GQNFEL PFG GRR+
Sbjct: 422 GYHIKKGTRLMPNLWKIHRDPSVWPDPLEFKPERFLSTHKDVDVRGQNFELLPFGSGRRM 481

Query: 492 CPGAGLGVQMTQLVLASLIHSVELGTRSDDLVDMAANFGLTIHRATPLQVLVKPRILASA 523
           C G  LG+ M   +LA+ +HS E+   S + +D+         +ATPL+VLVKP +    
Sbjct: 482 CAGMSLGLHMVHYILANFLHSFEILNPSPESIDVTEVLEFVTTKATPLEVLVKPCLSFKC 541

BLAST of Sgr027668 vs. ExPASy Swiss-Prot
Match: O49858 (Cytochrome P450 82A3 OS=Glycine max OX=3847 GN=CYP82A3 PE=2 SV=1)

HSP 1 Score: 523.5 bits (1347), Expect = 2.8e-147
Identity = 257/520 (49.42%), Postives = 355/520 (68.27%), Query Frame = 0

Query: 9   MEIILPYLN---SSIAGLFLLLLFSFFVLKKARACKRRQPPEAAGGWPIIGHLRLLGGSQ 68
           M+++L  LN    +IA +  L+    F+ +K    + +  P  +G WPI+GHL LL GSQ
Sbjct: 1   MDLLLNCLNLNTIAIASILSLIFLCLFLYRK--NSRGKDAPVVSGAWPILGHLSLLNGSQ 60

Query: 69  LPHETLGAMADKYGPIFSIRVGVHPSLVISSWEVAKECYTTLDSVVSSRPKSLGGKLLGY 128
            PH+ LGA+ADKYGP+F+I++G+ P+LV+S+WE++KE +TT D  VSSRPK +  +++ Y
Sbjct: 61  TPHKVLGALADKYGPLFTIKLGMKPALVLSNWEMSKELFTTNDLAVSSRPKLVAVEVMSY 120

Query: 129 NFAAFGFRPYDSFYRSIRKTIASEVLSNRRLELQRHIRVSEVKRSVKELYNLWTQ-REEG 188
           N A  G  PY  ++R +RK +  E LSNRR+E + HIRVSEV+ S+KEL+++W+   +  
Sbjct: 121 NQAFVGLAPYGPYWRELRKIVTFEFLSNRRIEQRNHIRVSEVRTSIKELFDIWSNGNKNE 180

Query: 189 SDHILIDADQWIGNINLNVILMMVCGKRFLGGSASDEKEMRQ-CRKVMRDFFDLTGQFTV 248
           S + L+D  QW+  +  N+++ MV GKR+ G    + K+  Q   K +R+F +L G FTV
Sbjct: 181 SRYTLVDIKQWLAYLTFNMVVRMVVGKRYFGVMHVEGKDKAQRFMKNIREFMNLMGTFTV 240

Query: 249 GDAIPFLRWLDLGGYAKAMKKTAKEMDCLVEEWLEEHRRRKRDSGATDGERDFMDVMLSI 308
            D +P LRWLDLGG+ KAMK  AKE+D L+ EWLEEHR++K      + +RDFMDVM+S 
Sbjct: 241 ADGVPCLRWLDLGGHEKAMKANAKEVDKLLSEWLEEHRQKKLLGENVESDRDFMDVMISA 300

Query: 309 LEEMDLAGYDADT-------SIISGGTDTITVTLTWAISLLLNNREALRRVQEELDIHVG 368
           L    +  +DADT        +I GGTD+  VTLTWA+SLLL N  AL + +EE+D+ +G
Sbjct: 301 LNGAQIGAFDADTICKATSLELILGGTDSTAVTLTWALSLLLRNPLALGKAKEEIDMQIG 360

Query: 369 NKRLVDESDLSKLVYLQAVVKETLRLYPAGPLSGVREFSRDCTVGGYDVAAGTRLITNLW 428
               + ESD+SKLVYLQA+VKETLRLYP  P S  REF+ +C +GGY +  GTRLI NLW
Sbjct: 361 KDEYIRESDISKLVYLQAIVKETLRLYPPAPFSSPREFTENCILGGYHIKKGTRLIHNLW 420

Query: 429 KIQTDPRVWPEPLEFRPERFLSSHQQLDVKGQNFELAPFGCGRRVCPGAGLGVQMTQLVL 488
           KI  DP VW +PLEF+PERFL++H+ +D++G NFEL PFG GRRVC G  LG+ M    L
Sbjct: 421 KIHRDPSVWSDPLEFKPERFLTTHKDVDLRGHNFELLPFGSGRRVCAGMSLGLNMVHFTL 480

Query: 489 ASLIHSVELGTRSDDLVDMAANFGLTIHRATPLQVLVKPR 517
           A+L+HS ++   S + VDM   FG T  +ATPL++LVKPR
Sbjct: 481 ANLLHSFDILNPSAEPVDMTEFFGFTNTKATPLEILVKPR 518

BLAST of Sgr027668 vs. ExPASy Swiss-Prot
Match: A0A0N7F297 (Demethylepipodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=CYP82D61 PE=1 SV=1)

HSP 1 Score: 516.9 bits (1330), Expect = 2.6e-145
Identity = 261/513 (50.88%), Postives = 341/513 (66.47%), Query Frame = 0

Query: 16  LNSSIAGLFLLL-LFSFFVLKKARACKRRQPPEAAGGWPIIGHLRLLGGSQLPHETLGAM 75
           L + + G F+LL  F +FV KK    K ++PP+ AG WPIIGHL LL    LPH+ L + 
Sbjct: 7   LETLLLGFFVLLPCFFYFVWKKPNN-KIKEPPQPAGAWPIIGHLHLLARGDLPHKILSSF 66

Query: 76  ADKYGPIFSIRVGVHPSLVISSWEVAKECYTTLDSVVSSRPKSLGGKLLGYNFAAFGFRP 135
           ADK GP+F I++GVH +LV+++ E+AKEC+TT D    +RP  +  K++GYN+   G  P
Sbjct: 67  ADKNGPVFKIQLGVHQALVVNNSEIAKECFTTNDRFFLNRPSGVAAKIMGYNYVMLGVAP 126

Query: 136 YDSFYRSIRKTIASEVLSNRRLELQRHIRVSEVKRSVKELYNLWTQREEGSDHILIDADQ 195
           Y  ++R +RK I  E LSNRRL+  +H+  SE+  S KELY LW    +  D  L+D  Q
Sbjct: 127 YGPYWRDMRKIIMLEFLSNRRLQSLKHVWHSEISISSKELYKLW--ETQNIDFCLVDMKQ 186

Query: 196 WIGNINLNVILMMVCGKRFLG---GSASDEKEMRQCRKVMRDFFDLTGQFTVGDAIPFLR 255
           W+ ++ LN+ + MV GKRF G    SA +E E   C K +R+ F L G F + D +P+LR
Sbjct: 187 WLADLTLNMSVKMVVGKRFFGSASASACEETESSNCPKTLRNMFRLMGSFVLSDYLPYLR 246

Query: 256 WLDLGGYAKAMKKTAKEMDCLVEEWLEEHRRRKRDSGATDGERDFMDVMLSILEEMDLAG 315
           WLDLGG+ K MK+T KE+D L + WL+EH+R++   G  D ++DFMDVMLSILEE  L G
Sbjct: 247 WLDLGGHEKEMKRTVKELDILFKGWLDEHKRKRLSGGKEDDDQDFMDVMLSILEESKL-G 306

Query: 316 YDADT-------SIISGGTDTITVTLTWAISLLLNNREALRRVQEELDIHVGNKRLVDES 375
            D DT       +II GG DT   TLTWA+SLLLNN  AL++ Q+ELD+HVG  R VDES
Sbjct: 307 NDVDTINKTACLAIILGGADTTWATLTWALSLLLNNPNALKKAQDELDLHVGRDRNVDES 366

Query: 376 DLSKLVYLQAVVKETLRLYPAGPLSGVREFSRDCTVGGYDVAAGTRLITNLWKIQTDPRV 435
           DL KL Y+ A++KETLRLYP GPL G R  + DCT+ GY V AGTRLI N WKIQ DP V
Sbjct: 367 DLVKLTYIDAIIKETLRLYPPGPLLGPRVVTEDCTIAGYHVRAGTRLIVNAWKIQRDPLV 426

Query: 436 WPEPLEFRPERFLSSHQQLDVKGQNFELAPFGCGRRVCPGAGLGVQMTQLVLASLIHSVE 495
           W +P E++PERFL   + +D+KGQ+FEL PFG GRR CP   L +Q+  L LA ++H  E
Sbjct: 427 WSQPHEYQPERFL--ERDVDMKGQHFELIPFGSGRRACPAISLALQVLPLTLAHILHGFE 486

Query: 496 LGTRSDDLVDMAANFGLTIHRATPLQVLVKPRI 518
           L T + + VDM    G+   +ATPL+VLV PRI
Sbjct: 487 LRTPNQNKVDMTETPGIVHAKATPLEVLVAPRI 513

BLAST of Sgr027668 vs. ExPASy TrEMBL
Match: A0A6J1DRH5 (cytochrome P450 CYP82D47-like OS=Momordica charantia OX=3673 GN=LOC111023576 PE=3 SV=1)

HSP 1 Score: 798.9 bits (2062), Expect = 1.3e-227
Identity = 400/534 (74.91%), Postives = 453/534 (84.83%), Query Frame = 0

Query: 5   TTSSMEIILPYLN-SSIAGLFLLLLFSFFVLKK-ARACKRRQPPEAAGGWPIIGHLRLLG 64
           T +SMEII PYLN +SI  LFLLLL S+ VLKK ++A KRR PP A G WPIIGHL LL 
Sbjct: 6   TATSMEIISPYLNTNSIIPLFLLLLISYLVLKKLSKASKRRAPPAAGGAWPIIGHLPLLS 65

Query: 65  GSQLPHETLGAMADKYGPIFSIRVGVHPSLVISSWEVAKECYTTLDSVVSSRPKSLGGKL 124
           G QLPHETLGAMADKYGPIF+I+VGVHP+LVISSWEVAKECYTT DS+VSSRPK++GGKL
Sbjct: 66  GPQLPHETLGAMADKYGPIFTIQVGVHPTLVISSWEVAKECYTTHDSIVSSRPKTVGGKL 125

Query: 125 LGYNFAAFGFRPYDSFYRSIRKTIASEVLSNRRLEL--QRHIRVSEVKRSVKELYNLWTQ 184
           LGYNFA FGFRPYDSFYRSIR+ IASE+LSNRRLEL  QR IR SEVKRSV+ELY+LW +
Sbjct: 126 LGYNFAGFGFRPYDSFYRSIRRIIASEMLSNRRLELQFQRDIRDSEVKRSVRELYDLWEK 185

Query: 185 REEGSDH----ILIDADQWIGNINLNVILMMVCGKRFLGGSASDEKEMRQCRKVMRDFFD 244
           + E SD     + +D D W+G+++L VILMMVCGKRF+G     EKEM++CR+ MR+FFD
Sbjct: 186 KRETSDDDDVAVNVDLDGWVGSVDLKVILMMVCGKRFVG-----EKEMKRCRRAMREFFD 245

Query: 245 LTGQFTVGDAIPFLRWLDLGGYAKAMKKTAKEMDCLVEEWLEEHRRRKRDSGATDGERDF 304
           L GQF VGDA+PFLRWLDLGGY KAMKKTAKE+D ++EEWL+EHRR+     A DG  DF
Sbjct: 246 LAGQFMVGDALPFLRWLDLGGYQKAMKKTAKELDSIMEEWLDEHRRKADSGAAADGGPDF 305

Query: 305 MDVMLSILEEMDLAGYDADT-------SIISGGTDTITVTLTWAISLLLNNREALRRVQE 364
           MDVMLS LE +DLAGYDA T       +II+GGTDT T T+ WAISLLLNNREAL+R QE
Sbjct: 306 MDVMLSTLEGIDLAGYDAATVNKATCMTIIAGGTDTTTETILWAISLLLNNREALKRAQE 365

Query: 365 ELDIHVGNKRLVDESDLSKLVYLQAVVKETLRLYPAGPLSGVREFSRDCTVGGYDVAAGT 424
           ELDIHVGNKRL+DESD+SKL YLQAVVKETLRLYPAGPLSG+REFSRDCTVGGYDVA GT
Sbjct: 366 ELDIHVGNKRLLDESDISKLEYLQAVVKETLRLYPAGPLSGIREFSRDCTVGGYDVAVGT 425

Query: 425 RLITNLWKIQTDPRVWPEPLEFRPERFLSSHQQLDVKGQNFELAPFGCGRRVCPGAGLGV 484
           RLITNLWKIQTDPRVW EPL+F+PERFLSSH+ LDVKGQNFE  PFGCGRRVCPG G G+
Sbjct: 426 RLITNLWKIQTDPRVWAEPLKFKPERFLSSHKHLDVKGQNFEFTPFGCGRRVCPGLGFGI 485

Query: 485 QMTQLVLASLIHSVELGTRSDDLVDMAANFGLTIHRATPLQVLVKPRILASAYA 524
           QMTQLVLASLIHS+EL TR D+ VDM A+FG+T+HR  PLQVL+KPR+LASAYA
Sbjct: 486 QMTQLVLASLIHSMELKTRFDEPVDMTADFGITMHRVNPLQVLIKPRLLASAYA 534

BLAST of Sgr027668 vs. ExPASy TrEMBL
Match: A0A6J1IHE0 (cytochrome P450 CYP82D47-like OS=Cucurbita maxima OX=3661 GN=LOC111476968 PE=3 SV=1)

HSP 1 Score: 786.2 bits (2029), Expect = 8.5e-224
Identity = 392/527 (74.38%), Postives = 447/527 (84.82%), Query Frame = 0

Query: 4   LTTSSMEIILPYLNSSIAGLFLLLLFSFFVLKKARACKRRQPPEAAGGWPIIGHLRLL-G 63
           LT  S+ II PYLN+ +A LFL LL SFFV KKA A KRR+PPE AGGWPIIGHL LL  
Sbjct: 3   LTGISIGIIPPYLNTFVAALFLFLLSSFFVFKKATAPKRREPPEVAGGWPIIGHLPLLKA 62

Query: 64  GSQLPHETLGAMADKYGPIFSIRVGVHPSLVISSWEVAKECYTTLDSVVSSRPKSLGGKL 123
            SQLPH+TLGA+ADKYGPIF IRVG  P+L+ISS E+A+EC++TLDS+V SRPKS+GGKL
Sbjct: 63  DSQLPHQTLGALADKYGPIFRIRVGAQPTLIISSSELARECHSTLDSIVCSRPKSVGGKL 122

Query: 124 LGYNFAAFGFRPYDSFYRSIRKTIASEVLSNRRLELQRHIRVSEVKRSVKELYNLWTQRE 183
           LGYN+AAFG RPYDSFYRS+RK + SEVLSNR L+L+R IRVSEVK+S+KELY+ WT RE
Sbjct: 123 LGYNYAAFGARPYDSFYRSMRKIVVSEVLSNRCLDLRRDIRVSEVKKSLKELYDQWTNRE 182

Query: 184 EGSDHILIDADQWIGNINLNVILMMVCGKRFLGGSASDEKEMRQCRKVMRDFFDLTGQFT 243
           EGSDHIL+D +QWIGNINL V+LMMVCGKRFLGGSA DE EM++CRK+MRDF DL G+F 
Sbjct: 183 EGSDHILVDIEQWIGNINLKVVLMMVCGKRFLGGSADDE-EMKRCRKIMRDFLDLMGKFL 242

Query: 244 VGDAIPFLRWLDLGGYAKAMKKTAKEMDCLVEEWLEEHRRRKRDSGATDGERDFMDVMLS 303
           VGD+IPFLRWLDLGGY KAMK ++K++D L+EEWLEEHRR++ D        D MDVMLS
Sbjct: 243 VGDSIPFLRWLDLGGYEKAMKISSKQLDSLLEEWLEEHRRKRTD------HNDLMDVMLS 302

Query: 304 ILEEMDLAGYDADT-------SIISGGTDTITVTLTWAISLLLNNREALRRVQEELDIHV 363
            LE MDLAGYDADT       SII+GGTDT+TVTL+WAISLLLNNREALR+ QEELDIHV
Sbjct: 303 NLEAMDLAGYDADTVNKATCLSIITGGTDTVTVTLSWAISLLLNNREALRKTQEELDIHV 362

Query: 364 GNKRLVDESDLSKLVYLQAVVKETLRLYPAGPLSGVREFSRDCTVGGYDVAAGTRLITNL 423
           G  RLVDESD+SKLVYLQAVV ETLRLYP GPLSGVR FS DCTVGGYDV AGT LIT+L
Sbjct: 363 GKDRLVDESDISKLVYLQAVVNETLRLYPPGPLSGVRVFSEDCTVGGYDVVAGTHLITDL 422

Query: 424 WKIQTDPRVWPEPLEFRPERFLSSHQQLDVKGQNFELAPFGCGRRVCPGAGLGVQMTQLV 483
           WKIQT+P+VW EPLEF+PERF+S  +QLDVKGQNFE APFG GRR CPG  LG+QMTQLV
Sbjct: 423 WKIQTNPQVWAEPLEFKPERFVSGEKQLDVKGQNFEFAPFGLGRRACPGRSLGIQMTQLV 482

Query: 484 LASLIHSVELGTRSDDLVDMAANFGLTIHRATPLQVLVKPRILASAY 523
           LASLIHS+ELGT SD+ VDM A FGLT++RA PLQ+LVKPR+LA AY
Sbjct: 483 LASLIHSLELGTGSDEPVDMTAGFGLTMYRAKPLQILVKPRLLACAY 522

BLAST of Sgr027668 vs. ExPASy TrEMBL
Match: A0A6J1FFB9 (cytochrome P450 CYP82D47-like OS=Cucurbita moschata OX=3662 GN=LOC111443560 PE=3 SV=1)

HSP 1 Score: 780.8 bits (2015), Expect = 3.6e-222
Identity = 390/523 (74.57%), Postives = 442/523 (84.51%), Query Frame = 0

Query: 8   SMEIILPYLNSSIAGLFLLLLFSFFVLKKARACKRRQPPEAAGGWPIIGHLRLL-GGSQL 67
           S+ II PYLN+ +A LFL LL SFFV KKA A KRR+PPE AGGWPIIGHL LL   SQL
Sbjct: 7   SIGIIPPYLNTFVAALFLFLLSSFFVFKKATAPKRREPPEVAGGWPIIGHLPLLKADSQL 66

Query: 68  PHETLGAMADKYGPIFSIRVGVHPSLVISSWEVAKECYTTLDSVVSSRPKSLGGKLLGYN 127
           PH+TLGA+ADKYGPIF IRVG  P+L+ISS E+A+EC++TLDS+V SRPKS+GGKLLGYN
Sbjct: 67  PHQTLGALADKYGPIFRIRVGAQPTLIISSSELARECHSTLDSIVCSRPKSVGGKLLGYN 126

Query: 128 FAAFGFRPYDSFYRSIRKTIASEVLSNRRLELQRHIRVSEVKRSVKELYNLWTQREEGSD 187
           +AAFG RPYDSFYRS+RK + SEVLSNR L L+R IRVSEVK+S+KELYN WT+REEGSD
Sbjct: 127 YAAFGARPYDSFYRSMRKIVVSEVLSNRCLGLRRDIRVSEVKKSLKELYNQWTKREEGSD 186

Query: 188 HILIDADQWIGNINLNVILMMVCGKRFLGGSASDEKEMRQCRKVMRDFFDLTGQFTVGDA 247
           HIL+D +QWIGN+NL V+LMMVCGKRFLG SA DE EM++CRKVMR+F DL G+F VGD+
Sbjct: 187 HILVDIEQWIGNVNLRVVLMMVCGKRFLGDSADDE-EMKRCRKVMREFLDLMGKFLVGDS 246

Query: 248 IPFLRWLDLGGYAKAMKKTAKEMDCLVEEWLEEHRRRKRDSGATDGERDFMDVMLSILEE 307
           IPFLRWLDLGGY KAMK T+K++D L+EEWLEEHRR++ D        D MDVMLS LE 
Sbjct: 247 IPFLRWLDLGGYEKAMKITSKQLDSLLEEWLEEHRRKRTD------HNDLMDVMLSNLEG 306

Query: 308 MDLAGYDADT-------SIISGGTDTITVTLTWAISLLLNNREALRRVQEELDIHVGNKR 367
           MDLAGYDADT       SII+GGTDT+TVTL+WAISLLLNNREALR+ QEELDIHVG  R
Sbjct: 307 MDLAGYDADTVNKATCLSIITGGTDTVTVTLSWAISLLLNNREALRKTQEELDIHVGKDR 366

Query: 368 LVDESDLSKLVYLQAVVKETLRLYPAGPLSGVREFSRDCTVGGYDVAAGTRLITNLWKIQ 427
            VDESD+SKLVYLQAVV ETLRLYP GPLSGVR FS DCTVGGYDV AGT LITNLWKIQ
Sbjct: 367 QVDESDISKLVYLQAVVNETLRLYPPGPLSGVRVFSEDCTVGGYDVVAGTHLITNLWKIQ 426

Query: 428 TDPRVWPEPLEFRPERFLSSHQQLDVKGQNFELAPFGCGRRVCPGAGLGVQMTQLVLASL 487
           T+P+VW EPLEF+PERF+S  +QLDVKGQNFE APFG GRR CPG  LG+QMTQLVLASL
Sbjct: 427 TNPQVWAEPLEFKPERFVSGEKQLDVKGQNFEFAPFGLGRRACPGMSLGIQMTQLVLASL 486

Query: 488 IHSVELGTRSDDLVDMAANFGLTIHRATPLQVLVKPRILASAY 523
           IHS+ELGT SD+ VDMAA FGLT++RA PLQVLVKPR+   AY
Sbjct: 487 IHSLELGTGSDEPVDMAAGFGLTMYRANPLQVLVKPRLFTCAY 522

BLAST of Sgr027668 vs. ExPASy TrEMBL
Match: A0A6J1FAF2 (cytochrome P450 CYP82D47-like OS=Cucurbita moschata OX=3662 GN=LOC111443559 PE=3 SV=1)

HSP 1 Score: 768.1 bits (1982), Expect = 2.4e-218
Identity = 384/532 (72.18%), Postives = 443/532 (83.27%), Query Frame = 0

Query: 5   TTSSMEIILPYLNSSIAG-LFLLLLFSFFVLKKARACKRR-QPPEAAGGWPIIGHLRLLG 64
           TTS  + I PYLNS++AG LFLLLLFS+FVLKKA + KRR QPPE AGGWPIIGHLRLL 
Sbjct: 13  TTSMADQISPYLNSTVAGLLFLLLLFSYFVLKKATSRKRRSQPPEVAGGWPIIGHLRLLS 72

Query: 65  -GSQLPHETLGAMADKYGPIFSIRVGVHPSLVISSWEVAKECYTTLDSVVSSRPKSLGGK 124
             SQL HETLG +ADKYGPIF IR+GV P+LVISSWEVA ECYTTLDS+VSSRPK +  K
Sbjct: 73  TDSQLLHETLGGLADKYGPIFGIRIGVRPALVISSWEVATECYTTLDSIVSSRPKIILQK 132

Query: 125 LLGYNFAAFGFRPY-DSFYRSIRKTIASEVLSNRRLELQRHIRVSEVKRSVKELYNLWTQ 184
            LGYNFA FGFR + DSFYR++RK +ASE LSNRRLELQR IRVSEVKRSVKE+Y+LW +
Sbjct: 133 KLGYNFAGFGFRHHCDSFYRNMRKIVASEALSNRRLELQRDIRVSEVKRSVKEVYSLWAK 192

Query: 185 REEGS--DHILIDADQWIGNINLNVILMMVCGKRFLGGSASDEKEMRQCRKVMRDFFDLT 244
             E S   ++++D D+WIGNI LNVILMMVCGKRF+GGS  DE+EM +CRK MR FFDL 
Sbjct: 193 TGEASSDSYLVLDLDEWIGNIALNVILMMVCGKRFVGGSCGDEREMERCRKAMRGFFDLG 252

Query: 245 GQFTVGDAIPFLRWLDLGGYAKAMKKTAKEMDCLVEEWLEEHRRRKRDSGATDGERDFMD 304
           G+F VGDAIPFL+WLDLGGY KA  K  KE+DCL+EEWLEEHRR+KRD    DGE D M 
Sbjct: 253 GKFIVGDAIPFLKWLDLGGYQKATNKVWKELDCLMEEWLEEHRRKKRDCSDADGEHDLMA 312

Query: 305 VMLSILEEMDLAGYDADT-------SIISGGTDTITVTLTWAISLLLNNREALRRVQEEL 364
           VM S+LE +DLAGYDADT       ++ISGGTDT+ VTLTWAISLLLNN+EALR+V+EEL
Sbjct: 313 VMPSLLEGLDLAGYDADTVNKATCLTLISGGTDTMAVTLTWAISLLLNNQEALRKVEEEL 372

Query: 365 DIHVGNKRLVDESDLSKLVYLQAVVKETLRLYPAGPLSGVREFSRDCTVGGYDVAAGTRL 424
           DIHVG +R VDESD+SKLVYLQAV+KETLRLYPAGPLSG RE S+DCT+GGY+VAAGT L
Sbjct: 373 DIHVGKERAVDESDISKLVYLQAVIKETLRLYPAGPLSGAREVSQDCTIGGYNVAAGTYL 432

Query: 425 ITNLWKIQTDPRVWPEPLEFRPERFLSSHQQLDVKGQNFELAPFGCGRRVCPGAGLGVQM 484
           ITN+WKIQ DPR+W EP EF+PERFLSSH+ +DVKGQ+FEL+PFG GRR CPG G+ + M
Sbjct: 433 ITNIWKIQRDPRIWQEPSEFKPERFLSSHKHMDVKGQHFELSPFGYGRRACPGQGISLLM 492

Query: 485 TQLVLASLIHSVELGTRSDDLVDMAANFGLTIHRATPLQVLVKPRILASAYA 524
           T LVLASL+HS +L TR D+ VDM AN GLT+HR  PLQVLVKPR+L +AYA
Sbjct: 493 TPLVLASLVHSFQLRTRRDEAVDMTANLGLTMHRVNPLQVLVKPRLLPTAYA 544

BLAST of Sgr027668 vs. ExPASy TrEMBL
Match: A0A0A0LGF0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G852560 PE=3 SV=1)

HSP 1 Score: 757.7 bits (1955), Expect = 3.3e-215
Identity = 380/531 (71.56%), Postives = 442/531 (83.24%), Query Frame = 0

Query: 1   MEFLTTSSMEIILPYLNSSIAGLFLLLLFSFFVLKKARACKRRQPPEAAGGWPIIGHLRL 60
           ME     S+ +I  YL S  AG FLL+L   FVLKKA A KR++PPE AGGWPIIGHLRL
Sbjct: 58  MEHPEIPSIGVISSYLYSFGAGFFLLVLSFLFVLKKATARKRKEPPEVAGGWPIIGHLRL 117

Query: 61  L-GGSQLPHETLGAMADKYGPIFSIRVGVHPSLVISSWEVAKECYTTLDSVVSSRPKSLG 120
           L   SQLPH+TLGA+ADKYGPIF IRVG  P+L+ISS E+AKEC+TTLDS+VSS PKS+ 
Sbjct: 118 LKSDSQLPHQTLGALADKYGPIFRIRVGAQPTLIISSSELAKECHTTLDSIVSSHPKSVA 177

Query: 121 GKLLGYNFAAFGFRPYDSFYRSIRKTIASEVLSNRRLELQRHIRVSEVKRSVKELYNLWT 180
           GKLLGYN+AAFG RPYDSFYR +RK +ASEVLSNRRLELQR +RVSEVK+++KE++N WT
Sbjct: 178 GKLLGYNYAAFGTRPYDSFYRRMRKIVASEVLSNRRLELQRDVRVSEVKKALKEVFNQWT 237

Query: 181 QREEGSDHILIDADQWIGNINLNVILMMVCGKRFLGGSASDEKEMRQCRKVMRDFFDLTG 240
           +REEGS+HIL+D ++ IGNINL V+LMMV GKRFLGGS   E EM++ RKVMRDF DL G
Sbjct: 238 KREEGSNHILVDVEELIGNINLKVVLMMVSGKRFLGGSGEVE-EMKRYRKVMRDFLDLLG 297

Query: 241 QFTVGDAIPFLRWLDLGGYAKAMKKTAKEMDCLVEEWLEEHRRRKRDSGATDGER-DFMD 300
           +F VGD+IPFLRWLD+GGY KAMK T+KE+D L+EEWLE+H RRKR+SGA DGE  D MD
Sbjct: 298 KFVVGDSIPFLRWLDVGGYEKAMKITSKELDSLLEEWLEDH-RRKRNSGAIDGEHGDLMD 357

Query: 301 VMLSILEEMDLAGYDAD-------TSIISGGTDTITVTLTWAISLLLNNREALRRVQEEL 360
           V+LS LE MDL GYDA+       TSII+GGTDT+T++L WA+SLLLNNRE LRR QEEL
Sbjct: 358 VLLSNLEGMDLGGYDANTVNKATCTSIITGGTDTVTISLAWAVSLLLNNREVLRRAQEEL 417

Query: 361 DIHVGNKRLVDESDLSKLVYLQAVVKETLRLYPAGPLSGVREFSRDCTVGGYDVAAGTRL 420
           DIHVGNKRLVDE D+SKLVYLQAVV ETLRLYP GPLSGVR FS DC VGGY++A GT L
Sbjct: 418 DIHVGNKRLVDELDISKLVYLQAVVNETLRLYPPGPLSGVRVFSEDCIVGGYNIAGGTHL 477

Query: 421 ITNLWKIQTDPRVWPEPLEFRPERFLSSHQQLDVKGQNFELAPFGCGRRVCPGAGLGVQM 480
           ITNLWKI T+P VW EPLEF+PERFL+ ++QLDVKGQ FE  PFGCGRR CPG  LG+QM
Sbjct: 478 ITNLWKIHTNPEVWAEPLEFKPERFLNRNKQLDVKGQRFEFLPFGCGRRSCPGMNLGIQM 537

Query: 481 TQLVLASLIHSVELGTRSDDLVDMAANFGLTIHRATPLQVLVKPRILASAY 523
           TQL+LASLIHS EL TRSD+ VDMAA+FG+ ++R  PL+VLVKPR+LASAY
Sbjct: 538 TQLMLASLIHSFELNTRSDEPVDMAASFGIAMYRTNPLEVLVKPRLLASAY 586

BLAST of Sgr027668 vs. TAIR 10
Match: AT4G31940.1 (cytochrome P450, family 82, subfamily C, polypeptide 4 )

HSP 1 Score: 525.8 bits (1353), Expect = 4.0e-149
Identity = 265/519 (51.06%), Postives = 364/519 (70.13%), Query Frame = 0

Query: 16  LNSSIAGLFL-LLLFSFFVL-KKARACKRRQPPEAAGGWPIIGHLRLLGG-SQLPHETLG 75
           +++S+  LF+ +L+F F  L KK++  K  + P  +G WPIIGHL LLGG  QL + TLG
Sbjct: 1   MDTSLFSLFVPILVFVFIALFKKSKKPKYVKAPAPSGAWPIIGHLHLLGGKEQLLYRTLG 60

Query: 76  AMADKYGPIFSIRVGVHPSLVISSWEVAKECYTTLDSVVSSRPKSLGGKLLGYNFAAFGF 135
            MAD YGP  S+++G + + V+SS+EVAK+C+T  D  ++SRP +   K +GYNFA FGF
Sbjct: 61  KMADHYGPAMSLQLGSNEAFVVSSFEVAKDCFTVNDKALASRPMTAAAKHMGYNFAVFGF 120

Query: 136 RPYDSFYRSIRKTIASEVLSNRRLELQRHIRVSEVKRSVKELYNLWTQREEGSDHILIDA 195
            PY +F+R +RK    E+LSNRRL++ +H+RVSE+   VK+LY+LW  +  G+  +++D 
Sbjct: 121 APYSAFWREMRKIATIELLSNRRLQMLKHVRVSEITMGVKDLYSLWF-KNGGTKPVMVDL 180

Query: 196 DQWIGNINLNVILMMVCGKRFLGG----SASDEKEMRQCRKVMRDFFDLTGQFTVGDAIP 255
             W+ ++ LN+I+ MV GKR+ GG    S+ D +E  QC+K +  FF L G FTV DA P
Sbjct: 181 KSWLEDMTLNMIVRMVAGKRYFGGGGSVSSEDTEEAMQCKKAIAKFFHLIGIFTVSDAFP 240

Query: 256 FLRWLDLGGYAKAMKKTAKEMDCLVEEWLEEHRRRKRDSGATDGERDFMDVMLSILEEMD 315
            L + DL G+ K MK+T  E+D ++E W+E HR++++ SG  + + DF+DVM+S+ E+  
Sbjct: 241 TLSFFDLQGHEKEMKQTGSELDVILERWIENHRQQRKFSGTKENDSDFIDVMMSLAEQGK 300

Query: 316 LA--GYDADTSI-------ISGGTDTITVTLTWAISLLLNNREALRRVQEELDIHVGNKR 375
           L+   YDA+TSI       I GG+DT   TLTWAISLLLNN+E L++ Q+E+DIHVG  R
Sbjct: 301 LSHLQYDANTSIKSTCLALILGGSDTSASTLTWAISLLLNNKEMLKKAQDEIDIHVGRDR 360

Query: 376 LVDESDLSKLVYLQAVVKETLRLYPAGPLSGVREFSRDCTVGGYDVAAGTRLITNLWKIQ 435
            V++SD+  LVYLQA++KETLRLYPAGPL G RE   DCTV GY V  GTRLI N+WKIQ
Sbjct: 361 NVEDSDIENLVYLQAIIKETLRLYPAGPLLGPREAMEDCTVAGYYVPCGTRLIVNVWKIQ 420

Query: 436 TDPRVWPEPLEFRPERFLSSH-QQLDVKGQNFELAPFGCGRRVCPGAGLGVQMTQLVLAS 495
            DP+V+ EP EFRPERF++   ++ DV+GQNFEL PFG GRR CPG+ L +Q+  L LA 
Sbjct: 421 RDPKVYMEPNEFRPERFITGEAKEFDVRGQNFELMPFGSGRRSCPGSSLAMQVLHLGLAR 480

Query: 496 LIHSVELGTRSDDLVDMAANFGLTIHRATPLQVLVKPRI 518
            +HS ++ T  D  VDM+ N GLTI +ATPL+VL+ PRI
Sbjct: 481 FLHSFDVKTVMDMPVDMSENPGLTIPKATPLEVLISPRI 518

BLAST of Sgr027668 vs. TAIR 10
Match: AT4G31970.1 (cytochrome P450, family 82, subfamily C, polypeptide 2 )

HSP 1 Score: 510.4 bits (1313), Expect = 1.7e-144
Identity = 255/523 (48.76%), Postives = 363/523 (69.41%), Query Frame = 0

Query: 16  LNSSIAGLFL-LLLFSFFVL-KKARACKRRQPPEAAGGWPIIGHLRLLGG-SQLPHETLG 75
           +++S+  LF+ +L+F F  L KK++  K  + P  +G WPIIGHL LL G  QL + TLG
Sbjct: 1   MDTSLFSLFVPILVFVFIALFKKSKKPKHVKAPAPSGAWPIIGHLHLLSGKEQLLYRTLG 60

Query: 76  AMADKYGPIFSIRVGVHPSLVISSWEVAKECYTTLDSVVSSRPKSLGGKLLGYNFAAFGF 135
            MAD+YGP  S+R+G   + V+SS+EVAK+C+T  D  ++SRP +   K +GY+ A FGF
Sbjct: 61  KMADQYGPAMSLRLGSSETFVVSSFEVAKDCFTVNDKALASRPITAAAKHMGYDCAVFGF 120

Query: 136 RPYDSFYRSIRKTIASEVLSNRRLELQRHIRVSEVKRSVKELYNLWTQREEGSDHILIDA 195
            PY +F+R +RK    E+LSNRRL++ +H+RVSE+   +++LY+LW  ++ GS+ +++D 
Sbjct: 121 APYSAFWREMRKIATLELLSNRRLQMLKHVRVSEISMVMQDLYSLWV-KKGGSEPVMVDL 180

Query: 196 DQWIGNINLNVILMMVCGKRFLGG---SASDEKEMRQCRKVMRDFFDLTGQFTVGDAIPF 255
             W+ +++LN+++ MV GKR+ GG   S  D +E RQCRK + +FF L G FTV DA P 
Sbjct: 181 KSWLEDMSLNMMVRMVAGKRYFGGGSLSPEDAEEARQCRKGVANFFHLVGIFTVSDAFPK 240

Query: 256 LRWLDLGGYAKAMKKTAKEMDCLVEEWLEEHRRRKRDSGATDGERDFMDVMLSILEEMDL 315
           L W D  G+ K MK+T +E+D ++E W+E HR++++ SG    + DF+DVMLS+ E+   
Sbjct: 241 LGWFDFQGHEKEMKQTGRELDVILERWIENHRQQRKVSGTKHNDSDFVDVMLSLAEQGKF 300

Query: 316 A--GYDADTSI-------ISGGTDTITVTLTWAISLLLNNREALRRVQEELDIHVGNKRL 375
           +   +DA TSI       I GG++T   TLTWAISLLLNN++ L++ Q+E+DIHVG  R 
Sbjct: 301 SHLQHDAITSIKSTCLALILGGSETSPSTLTWAISLLLNNKDMLKKAQDEIDIHVGRDRN 360

Query: 376 VDESDLSKLVYLQAVVKETLRLYPAGPLSGVREFSRDCTVGGYDVAAGTRLITNLWKIQT 435
           V++SD+  LVY+QA++KETLRLYPAGPL G RE   DCTV GY+V  GTR++ N+WKIQ 
Sbjct: 361 VEDSDIENLVYIQAIIKETLRLYPAGPLLGHREAIEDCTVAGYNVRRGTRMLVNVWKIQR 420

Query: 436 DPRVWPEPLEFRPERFLSSH-QQLDVKGQNFELAPFGCGRRVCPGAGLGVQMTQLVLASL 495
           DPRV+ EP EFRPERF++   ++ DV+GQNFEL PFG GRR CPG+ L +Q+  L LA  
Sbjct: 421 DPRVYMEPNEFRPERFITGEAKEFDVRGQNFELMPFGSGRRSCPGSSLAMQVLHLGLARF 480

Query: 496 IHSVELGTRSDDLVDMAANFGLTIHRATPLQVLVKPRILASAY 523
           + S ++ T  D  VDM  + GLTI +ATPL++L+ PR+    Y
Sbjct: 481 LQSFDVKTVMDMPVDMTESPGLTIPKATPLEILISPRLKEGLY 522

BLAST of Sgr027668 vs. TAIR 10
Match: AT4G31950.1 (cytochrome P450, family 82, subfamily C, polypeptide 3 )

HSP 1 Score: 474.9 bits (1221), Expect = 8.1e-134
Identity = 253/519 (48.75%), Postives = 349/519 (67.24%), Query Frame = 0

Query: 16  LNSSIAGLFL-LLLFSFFVL-KKARACKRRQPPEAAGGWPIIGHLRLLGG-SQLPHETLG 75
           +++S+  LF+ +L+F F  L KK++  K  + P  +G WPIIGHL LLGG  QL + TLG
Sbjct: 1   MDTSLFSLFVSILVFVFIALFKKSKKPKYVKAPAPSGAWPIIGHLHLLGGKEQLLYRTLG 60

Query: 76  AMADKYGPIFSIRVGVHPSLVISSWEVAKECYTTLDSVVSSRPKSLGGKLLGYNFAAFGF 135
            MAD YGP  S+R+G   + V SS+EVAK+C+T  D  ++S   +   K +GY       
Sbjct: 61  KMADHYGPAMSLRLGSSETFVGSSFEVAKDCFTVNDKALASL-MTAAAKHMGY------- 120

Query: 136 RPYDSFYRSIRKTIASEVLSNRRLELQRHIRVSEVKRSVKELYNLWTQREEGSDHILIDA 195
                F+  +RK    E+LSNRRL++  ++RVSE+   VK+LY+LW  ++ GS+ +++D 
Sbjct: 121 ----VFWLEMRKIAMIELLSNRRLQMLNNVRVSEISMGVKDLYSLWV-KKGGSEPVMVDL 180

Query: 196 DQWIGNINLNVILMMVCGKRFLGG----SASDEKEMRQCRKVMRDFFDLTGQFTVGDAIP 255
             W+ ++  N+I+ MV GKR+ GG    S+   +E RQ RK +  FF L G FTV DA P
Sbjct: 181 KSWLEDMIANMIMRMVAGKRYFGGGGAESSEHTEEARQWRKGIAKFFHLVGIFTVSDAFP 240

Query: 256 FLRWLDLGGYAKAMKKTAKEMDCLVEEWLEEHRRRKRDSGATDGERDFMDVMLSILEEMD 315
            L WLDL G+ K MK+T +E+D ++E W+E HR++++ SG    + DF+DVMLS+ E+  
Sbjct: 241 KLGWLDLQGHEKEMKQTRRELDVILERWIENHRQQRKVSGTKHNDSDFVDVMLSLAEQGK 300

Query: 316 LA--GYDADT-------SIISGGTDTITVTLTWAISLLLNNREALRRVQEELDIHVGNKR 375
           L+   YDA+T       ++I GG++T   TLTWAISLLLNN++ L++VQ+E+DIHVG  R
Sbjct: 301 LSHLQYDANTCIKTTCLALILGGSETSPSTLTWAISLLLNNKDMLKKVQDEIDIHVGRDR 360

Query: 376 LVDESDLSKLVYLQAVVKETLRLYPAGPLSGVREFSRDCTVGGYDVAAGTRLITNLWKIQ 435
            V++SD+  LVYLQA++KETLRLYPA PL G RE   DCTV GY+V  GTRLI N+WKIQ
Sbjct: 361 NVEDSDIKNLVYLQAIIKETLRLYPAAPLLGHREAMEDCTVAGYNVPCGTRLIVNVWKIQ 420

Query: 436 TDPRVWPEPLEFRPERFLSSH-QQLDVKGQNFELAPFGCGRRVCPGAGLGVQMTQLVLAS 495
            DP+V+ EP EFRPERF++   +  DV+GQNFEL PFG GRR CPG  L +QM  L LA 
Sbjct: 421 RDPKVYMEPNEFRPERFITGEAKDFDVRGQNFELMPFGSGRRSCPGPSLAMQMLHLGLAR 480

Query: 496 LIHSVELGTRSDDLVDMAANFGLTIHRATPLQVLVKPRI 518
            +HS E+ T  D  VDM+ + GLTI +ATPL+VL+ PR+
Sbjct: 481 FLHSFEVKTVLDRPVDMSESPGLTITKATPLEVLINPRL 506

BLAST of Sgr027668 vs. TAIR 10
Match: AT2G25160.1 (cytochrome P450, family 82, subfamily F, polypeptide 1 )

HSP 1 Score: 380.2 bits (975), Expect = 2.7e-105
Identity = 211/528 (39.96%), Postives = 306/528 (57.95%), Query Frame = 0

Query: 9   MEIILPYLNSSIAGLFLLLLFSFFVLKKAR---ACKRRQPPEAAGGWPIIGHLRLLGGSQ 68
           M++I+ +L S+      L +F   +L K+R     K+   P   G WP++GHL L     
Sbjct: 1   MDLIMLFLLSA------LFIFPVLILIKSRLRPKNKKSTAPMVPGAWPLLGHLHLFDTVN 60

Query: 69  LPHETLGAMADKYGPIFSIRVGVHPSLVISSWEVAKECYTTLDSVVSSRPKSLGGKLLGY 128
             H T GAMAD YGP+F  ++G    ++I+S EVAKE YT  D ++  RP+    KLLGY
Sbjct: 61  PTHVTFGAMADVYGPVFMAKLGSIKVMIINSKEVAKEIYTVHDKLL-ERPELTASKLLGY 120

Query: 129 NFAAFGFRPYDSFYRSIRKTIASEVLSNRRLELQRHIRVSEVKRSVKELYNLWTQREEGS 188
           N +   F PY  ++R IRK   SE+ S   +++    R  E   + + LY  W +R +  
Sbjct: 121 NDSFLTFSPYGLYWREIRKIAVSELFSTSGVDMHMVSRAREADLAFRALYVRWEKRGKPK 180

Query: 189 DHILIDADQWIGNINLNVILMMVCGKRFLGGSASDE-KEMRQCRKVMRDFFDLTGQFTVG 248
           + +L+D  Q   ++  N+ LMMV GKR+ G + + E KE R+C K++R+F D    F + 
Sbjct: 181 EGVLVDMKQEFIDLTANISLMMVSGKRYFGENPNCEVKEARRCGKLIREFLDYFALFLLS 240

Query: 249 DAIPFLRWLDLGGYAKAMKKTAKEMDCLVEEWLEEHRRRKRDSGATDGERDFMDVMLSIL 308
           D  P L +LD     + MK+TAK +D + E W+EEH+ ++ D G +  E D++D+++ IL
Sbjct: 241 DVAPVLGFLD-WKTKRGMKRTAKGLDKVAEGWIEEHKNKRSDHGRS--ENDYLDILIKIL 300

Query: 309 EEMDLAGY-DADTSI-------ISGGTDTITVTLTWAISLLLNNREALRRVQEELDIHVG 368
            +  + G  D  T I       +  G++T  V L WA+SLLLNN   LR+ QEELD  +G
Sbjct: 301 GQDKIPGLSDTHTKIKALCLNLVLAGSETAIVVLVWAVSLLLNNPHVLRKAQEELDSKIG 360

Query: 369 NKRLVDESDLSKLVYLQAVVKETLRLYPAGPLSGVREFSRDCTVG--GYDVAAGTRLITN 428
            +R+V+E D+  LVYLQA+VKET RLYP  PL   R    D  +      V AGT+L+ +
Sbjct: 361 KERVVEELDIKDLVYLQAIVKETFRLYPPVPLVAYRAVVEDFDIAFCKCHVPAGTQLMVS 420

Query: 429 LWKIQTDPRVWPEPLEFRPERFLSSHQQLDVKGQNFELAPFGCGRRVCPGAGLGVQMTQL 488
            WKI  DP VW  P +F PERFL+S+++LDV GQ+++  PFG GRR CP   LG++M   
Sbjct: 421 AWKIHRDPNVWSNPEQFEPERFLTSNRELDVGGQSYKFFPFGLGRRSCPAIPLGMRMVHY 480

Query: 489 VLASLIHSVELGTRSDDLVDMAANFGLTIHRATPLQVLVKPRILASAY 523
           +L   +HS +L   S   VDM  + GL  H+ATPL+V + PR+  S Y
Sbjct: 481 LLVRFLHSFDLARPSSQDVDMTESNGLVNHKATPLEVNIIPRLHKSLY 518

BLAST of Sgr027668 vs. TAIR 10
Match: AT3G25180.1 (cytochrome P450, family 82, subfamily G, polypeptide 1 )

HSP 1 Score: 363.6 bits (932), Expect = 2.6e-100
Identity = 205/526 (38.97%), Postives = 298/526 (56.65%), Query Frame = 0

Query: 9   MEIILPYLNSSIAGLFLLLLFSFFVLKKARACK--RRQPPEAAGGWPIIGHLRLLGGSQL 68
           M  +   L  S+  L L++    F+ K+   C+      PE  G  P+ GHL LL G +L
Sbjct: 1   MTFLFSTLQLSLFSLALVIFGYIFLRKQLSRCEVDSSTIPEPLGALPLFGHLHLLRGKKL 60

Query: 69  PHETLGAMADKYGPIFSIRVGVHPSLVISSWEVAKECYTTLDSVVSSRPKSLGGKLLGYN 128
             + L AM+ K+GPIFS+++G +  +V S  +  K+C+TT D   ++RP    G+ +GYN
Sbjct: 61  LCKKLAAMSQKHGPIFSLKLGFYRLVVASDPKTVKDCFTTNDLATATRPNIAFGRYVGYN 120

Query: 129 FAAFGFRPYDSFYRSIRKTIASEVLSNRRLELQRHIRVSEVKRSVKELYNLWTQREEGSD 188
            A+    PY  ++R +RK +   + SN  +E+  HIR SEV   +K LY     +  G  
Sbjct: 121 NASLTLAPYGDYWRELRKIVTVHLFSNHSIEMLGHIRSSEVNTLIKHLY-----KGNGGT 180

Query: 189 HILIDADQWIGNINLNVILMMVCGKRF-LGGSASDEKEMRQCRKVMRDFFDLTGQFTVGD 248
            I +  D     +  N+IL  + GKR   G   SDE   ++  K       +     +GD
Sbjct: 181 SI-VKIDMLFEFLTFNIILRKMVGKRIGFGEVNSDEWRYKEALKHCEYLAVIP---MIGD 240

Query: 249 AIPFLRWLDLGGYAKAMKKTAKEMDCLVEEWLEEHRRRKRDSGATDGERDFMDVMLSIL- 308
            IP+L WLD    ++ MK+  KE+D +  +WL EH  +KR     D ER  MD++L IL 
Sbjct: 241 VIPWLGWLDFAKNSQ-MKRLFKELDSVNTKWLHEH-LKKRSRNEKDQERTIMDLLLDILP 300

Query: 309 EEMDLAGYDADT-------SIISGGTDTITVTLTWAISLLLNNREALRRVQEELDIHVGN 368
           E++ ++G+  D        ++   G+D+ ++TLTWA+SLLLNN  AL   QEE+D  VG 
Sbjct: 301 EDIVISGHVRDVIVKATILALTLTGSDSTSITLTWAVSLLLNNPAALEAAQEEIDNSVGK 360

Query: 369 KRLVDESDLSKLVYLQAVVKETLRLYPAGPLSGVREFSRDCTVGGYDVAAGTRLITNLWK 428
            R ++ESD+  L YLQA+VKET RLYP  PL+G+RE   DC VGGY V  GTRL+ N+WK
Sbjct: 361 GRWIEESDIQNLKYLQAIVKETHRLYPPAPLTGIREAREDCFVGGYRVEKGTRLLVNIWK 420

Query: 429 IQTDPRVWPEPLEFRPERFLSSHQQLDVKGQNFELAPFGCGRRVCPGAGLGVQMTQLVLA 488
           +  DP++WP+P  F+PERF+    Q +    NFE  PFG GRR CPG  LG+++   VLA
Sbjct: 421 LHRDPKIWPDPKTFKPERFMEDKSQCE--KSNFEYIPFGSGRRSCPGVNLGLRVVHFVLA 480

Query: 489 SLIHSVELGTRSDDLVDMAANFGLTIHRATPLQVLVKPRILASAYA 524
            L+   EL   SD+ +DMA   GL + +  P++V+V PR+    Y+
Sbjct: 481 RLLQGFELHKVSDEPLDMAEGPGLALPKINPVEVVVMPRLDPKLYS 513