Sgr023071 (gene) Monk fruit (Qingpiguo) v1

BLAST of Sgr023071 vs. NCBI nr
Match: XP_022143967.1 (acetylajmalan esterase-like, partial [Momordica charantia])

HSP 1 Score: 303.1 bits (775), Expect = 2.3e-78
Identity = 166/254 (65.35%), Postives = 181/254 (71.26%), Query Frame = 0

Query: 8   VLALALLCSSFLLLLLPSTFNAHPLKACMFDAIYQLGDSISDTGNLIRENPATPFSHLPY 67
           +L+  LLCSS   LLLPS  NAH LKAC FDAIYQLGDSISDTGNLIRENP TPFSHLPY
Sbjct: 2   MLSFLLLCSS---LLLPSPSNAHLLKACKFDAIYQLGDSISDTGNLIRENPVTPFSHLPY 61

Query: 68  GETFFKNATGRCSNGLLMIDHFVHCNNQYALSFCSFGGWTSSDQPVFEQRGIDESWGEFC 127
           GETFFKNATGRCSNGLLMID F       A        + + D               F 
Sbjct: 62  GETFFKNATGRCSNGLLMIDQFA-----LAAGLPLINPYLNKD-------------ASFT 121

Query: 128 SGWLYGFAFGSSCS------KEISSPVTNSSLTKQLDWMSAHFNTTCSHQRDCTEKLKNA 187
            G  +  A  ++ S      K I SP TNSSLTKQLDWMS HF+T CSH +     L +A
Sbjct: 122 HGVNFAVAGSTALSSQVLAQKMILSPATNSSLTKQLDWMSTHFDTICSHHKGL--YLNDA 181

Query: 188 LFLVGEIGGNDYNYALFQGKTVEEVRNMVPEVVQTIKDAVTRVISYGAARVLVPGNFPIG 247
           LFLVGEIGGNDYNYALFQGKT+EEV+NMVPEVVQTIKDA+ +VISYGA RV+VPGNFPIG
Sbjct: 182 LFLVGEIGGNDYNYALFQGKTIEEVKNMVPEVVQTIKDAIIKVISYGAVRVIVPGNFPIG 232

Query: 248 CLPIYLTGFQTKDS 256
           CLPIYLTGFQTK S
Sbjct: 242 CLPIYLTGFQTKIS 232

BLAST of Sgr023071 vs. NCBI nr
Match: XP_038877043.1 (LOW QUALITY PROTEIN: GDSL esterase/lipase At5g03980-like, partial [Benincasa hispida])

HSP 1 Score: 301.6 bits (771), Expect = 6.6e-78
Identity = 159/255 (62.35%), Postives = 179/255 (70.20%), Query Frame = 0

Query: 1   MAVSTKSVLALALLCSSFLLLLLPSTFNAHPLKACMFDAIYQLGDSISDTGNLIRENPAT 60
           MA STKS     L  S FL L L S+FNAH LK CMFDAIYQLGDSISDTGNLIREN  T
Sbjct: 1   MAASTKS-----LWISIFLFLFLSSSFNAHLLKVCMFDAIYQLGDSISDTGNLIRENLNT 60

Query: 61  PFSHLPYGETFFKNATGRCSNGLLMIDHFVHCNNQYALSFCSFGGWTSSDQPVFEQRGID 120
           PFSHLPYGE+FF N TGRCSNGLLMID+F            +         P   +  + 
Sbjct: 61  PFSHLPYGESFFNNPTGRCSNGLLMIDYF------------ALDAGLPLVSPYLNKDALT 120

Query: 121 ESWGEFCSGWLYGFAFGSSCSKEISSPVTNSSLTKQLDWMSAHFNTTCSHQRDCTEKLKN 180
                F        +      K+ISSPVTNSSL+ QLDWM +HFN+ C +QRDC EKL N
Sbjct: 121 NHGVNFAVAGSTALSSELLFQKKISSPVTNSSLSHQLDWMFSHFNSICYNQRDCNEKLYN 180

Query: 181 ALFLVGEIGGNDYNYALFQGKTVEEVRNMVPEVVQTIKDAVTRVISYGAARVLVPGNFPI 240
           ALFLVGEIGGNDYNYALFQGKT++EV++MVP+VVQ+IK AV RVISYGA RV+VPGNFPI
Sbjct: 181 ALFLVGEIGGNDYNYALFQGKTIQEVKDMVPDVVQSIKSAVERVISYGATRVVVPGNFPI 238

Query: 241 GCLPIYLTGFQTKDS 256
           GC PIYLT FQT D+
Sbjct: 241 GCFPIYLTAFQTNDT 238

BLAST of Sgr023071 vs. NCBI nr
Match: XP_011659746.1 (acetylajmalan esterase-like [Cucumis sativus])

HSP 1 Score: 295.0 bits (754), Expect = 6.2e-76
Identity = 157/256 (61.33%), Postives = 185/256 (72.27%), Query Frame = 0

Query: 1   MAVSTKSVLALALLCSSFLLLLLPSTFNAHPLKACMFDAIYQLGDSISDTGNLIRENPAT 60
           MA STKS L+L LL    LL+L+  +  AHPLKACMFDAIYQLGDSISDTGNLIRENP T
Sbjct: 1   MAPSTKSSLSLFLL----LLVLVLGSSKAHPLKACMFDAIYQLGDSISDTGNLIRENPNT 60

Query: 61  PFSHLPYGETFFKNATGRCSNGLLMIDHFVHCNNQYALSFCSFGGWTSSDQPVFEQRGID 120
           PFSHLPYG++FF N TGRCSNGLLM+D            F +         P   + G+ 
Sbjct: 61  PFSHLPYGQSFFNNPTGRCSNGLLMLD------------FLALDAGLPLVSPYLNKDGLM 120

Query: 121 ESWGEFC-SGWLYGFAFGSSCSKEISSPVTNSSLTKQLDWMSAHFNTTCSHQRDCTEKLK 180
           +    F  +G     +   S S +I SPVTNSSL  QLDWM +HFN+ C +QR+C EKL+
Sbjct: 121 DHGVNFAVAGSTALPSQYLSSSYKIISPVTNSSLDHQLDWMFSHFNSICHNQRECNEKLR 180

Query: 181 NALFLVGEIGGNDYNYALFQGKTVEEVRNMVPEVVQTIKDAVTRVISYGAARVLVPGNFP 240
           +ALFLVGEIGGNDYNYALFQGKT++E ++MVP+VVQTIK AV +VISYGA RV+VPGNFP
Sbjct: 181 SALFLVGEIGGNDYNYALFQGKTIQEAKDMVPDVVQTIKSAVEKVISYGATRVVVPGNFP 240

Query: 241 IGCLPIYLTGFQTKDS 256
           IGC PIYLTGF T D+
Sbjct: 241 IGCFPIYLTGFHTNDT 240

BLAST of Sgr023071 vs. NCBI nr
Match: XP_022156428.1 (acetylajmalan esterase-like [Momordica charantia])

HSP 1 Score: 293.5 bits (750), Expect = 1.8e-75
Identity = 151/253 (59.68%), Postives = 178/253 (70.36%), Query Frame = 0

Query: 9   LALALLCSSF------LLLLLPSTFNAHPLKACMFDAIYQLGDSISDTGNLIRENPATPF 68
           +A++   SSF      L LLL S   AHPL+ACMFDAIYQLGDSISDTGNLIRENP T F
Sbjct: 1   MAISFTTSSFSLLLPLLFLLLSSASTAHPLRACMFDAIYQLGDSISDTGNLIRENPNTTF 60

Query: 69  SHLPYGETFFKNATGRCSNGLLMIDHFVHCNNQYALSFCSFGGWTSSDQPVFEQRGIDES 128
           SHLPYGETFF  ATGRCSNGLLM+D+F            +         P   +  +   
Sbjct: 61  SHLPYGETFFHGATGRCSNGLLMLDYF------------ALDAGLPLVNPYLNRDALTNH 120

Query: 129 WGEFCSGWLYGFAFGSSCSKEISSPVTNSSLTKQLDWMSAHFNTTCSHQRDCTEKLKNAL 188
              F        +F +    +I SPVTNSSL  QL WM +HF + C ++RDC EKL++AL
Sbjct: 121 GVNFAVAGSTALSFEALLRNKILSPVTNSSLDHQLGWMHSHFESICLNERDCIEKLRSAL 180

Query: 189 FLVGEIGGNDYNYALFQGKTVEEVRNMVPEVVQTIKDAVTRVISYGAARVLVPGNFPIGC 248
           FLVGEIGGNDYNYALFQGKT++EV++MVP+VVQTIK+AV +VISYGA RV+VPGNFPIGC
Sbjct: 181 FLVGEIGGNDYNYALFQGKTIQEVKDMVPDVVQTIKNAVEKVISYGATRVIVPGNFPIGC 240

Query: 249 LPIYLTGFQTKDS 256
           LPIYLTGFQT D+
Sbjct: 241 LPIYLTGFQTNDT 241

BLAST of Sgr023071 vs. NCBI nr
Match: KGN60996.2 (hypothetical protein Csa_021157 [Cucumis sativus])

HSP 1 Score: 293.1 bits (749), Expect = 2.4e-75
Identity = 156/257 (60.70%), Postives = 184/257 (71.60%), Query Frame = 0

Query: 1   MAVSTKSVLALALLCSSFLLLLLPSTFNAHPLKACMFDAIYQLGDSISDTGNLIRENPAT 60
           MA STKS  +L LL    LLL++  +  AHPLKACMFDAIYQLGDSISDTGNLIRENP T
Sbjct: 1   MAASTKSSFSLFLL----LLLVVLGSSKAHPLKACMFDAIYQLGDSISDTGNLIRENPNT 60

Query: 61  PFSHLPYGETFFKNATGRCSNGLLMIDHF-VHCNNQYALSFCSFGGWTSSDQPV-FEQRG 120
           PFSHLPYG++FF N TGRCSNGLLM+D F +         + +  GW   D  V F   G
Sbjct: 61  PFSHLPYGQSFFNNPTGRCSNGLLMLDFFALDAGLPLVTPYLNKDGW--MDHGVNFAVAG 120

Query: 121 IDESWGEFCSGWLYGFAFGSSCSKEISSPVTNSSLTKQLDWMSAHFNTTCSHQRDCTEKL 180
                 ++            S + +I SPVTNSSL  QL+WM +HFN+ C  QRDC EKL
Sbjct: 121 STALPSQYL-----------STNYKILSPVTNSSLDHQLEWMFSHFNSICHDQRDCNEKL 180

Query: 181 KNALFLVGEIGGNDYNYALFQGKTVEEVRNMVPEVVQTIKDAVTRVISYGAARVLVPGNF 240
           ++ALFLVGEIGGNDYNYALFQGKT++E ++MVP+VV+TIK AV +VISYGA RV+VPGNF
Sbjct: 181 RSALFLVGEIGGNDYNYALFQGKTIQEAKHMVPDVVRTIKSAVEKVISYGATRVVVPGNF 240

Query: 241 PIGCLPIYLTGFQTKDS 256
           PIGC PIYLTGF T D+
Sbjct: 241 PIGCFPIYLTGFHTNDT 240

BLAST of Sgr023071 vs. ExPASy Swiss-Prot
Match: Q9FXJ1 (GDSL esterase/lipase At1g28570 OS=Arabidopsis thaliana OX=3702 GN=At1g28570 PE=3 SV=1)

HSP 1 Score: 153.3 bits (386), Expect = 3.8e-36
Identity = 97/272 (35.66%), Postives = 138/272 (50.74%), Query Frame = 0

Query: 1   MAVSTKSVLALALLCSSFLLLLLPSTFNAHPLKACMFDAIYQLGDSISDTGNLIRENPAT 60
           MA     +++  L+ S+F L    +T N+ P +   F +I   GDSI+DTGNL+  +  T
Sbjct: 1   MATLFMKLVSFFLILSTFCL----TTVNSEP-QCHNFKSIISFGDSIADTGNLLALSDPT 60

Query: 61  ---PFSHLPYGETFFKNATGRCSNGLLMIDHFV---------------HCNNQYALSFCS 120
                + LPYGETFF + TGR SNG L+ID                  + N +  ++F +
Sbjct: 61  NLPKVAFLPYGETFFHHPTGRFSNGRLIIDFIAEFLGFPLVPPFYGSQNANFEKGVNF-A 120

Query: 121 FGGWTSSDQPVFEQRGIDESWGEFCSGWLYGFAFGSSCSKEISSPVTNSSLTKQLDWMSA 180
            GG T+ ++   E+RG                         I  P TN SL  QL     
Sbjct: 121 VGGATALERSFLEERG-------------------------IHFPYTNVSLAVQLSSFKE 180

Query: 181 HFNTTCSHQRDCTEKLKNALFLVGEIGGNDYNYALFQGKTVEEVRNMVPEVVQTIKDAVT 240
                C    DC + ++N+L L+GEIGGNDYNYA F GK +EE++ +VP V++TI  A+T
Sbjct: 181 SLPNLCVSPSDCRDMIENSLILMGEIGGNDYNYAFFVGKNIEEIKELVPLVIETISSAIT 240

Query: 241 RVISYGAARVLVPGNFPIGCLPIYLTGFQTKD 255
            +I  G    LVPG FP+GC   YL+ +QT +
Sbjct: 241 ELIGMGGKTFLVPGEFPLGCSVAYLSLYQTSN 241

BLAST of Sgr023071 vs. ExPASy Swiss-Prot
Match: Q3MKY2 (Acetylajmalan esterase OS=Rauvolfia serpentina OX=4060 GN=AAE PE=1 SV=1)

HSP 1 Score: 152.5 bits (384), Expect = 6.5e-36
Identity = 83/212 (39.15%), Postives = 114/212 (53.77%), Query Frame = 0

Query: 35  CMFDAIYQLGDSISDTGNLIR---ENPATPFSHLPYGETFFKNATGRCSNGLLMIDHFVH 94
           C FD+IYQLGDS SDTGNLIR   + P    +H PYGETF    TGRCS+G L+ID    
Sbjct: 25  CPFDSIYQLGDSFSDTGNLIRLPPDGPTFTAAHFPYGETFPGTPTGRCSDGRLIIDFIAT 84

Query: 95  CNNQYALSFCSFGGWTSSDQPVFEQRGIDESWGEFCSGWLYGFAFGSSCSKEISSPVTNS 154
             N   L+            P  +Q         F              ++ +     +S
Sbjct: 85  ALNLPLLN------------PYLQQNVSFRHGVNFAVAGATALDRSFLAARGVQVSDIHS 144

Query: 155 SLTKQLDWMSAHFNTTCSHQRDCTEKLKNALFLVGEIGGNDYNYALFQGKTVEEVRNMVP 214
            L+ QL+W   +  + CS  ++C+ KLKNALF++G IG ND NYA F  +T+EE+R  VP
Sbjct: 145 HLSAQLNWFRTYLGSICSTPKECSNKLKNALFILGNIGNNDVNYA-FPNRTIEEIRAYVP 204

Query: 215 EVVQTIKDAVTRVISYGAARVLVPGNFPIGCL 244
            + + + +A   +I  G +RV+VPG FPIGC+
Sbjct: 205 FITEAVANATREIIRLGGSRVIVPGIFPIGCV 223

BLAST of Sgr023071 vs. ExPASy Swiss-Prot
Match: Q9LZB2 (GDSL esterase/lipase At5g03980 OS=Arabidopsis thaliana OX=3702 GN=At5g03980 PE=2 SV=1)

HSP 1 Score: 147.1 bits (370), Expect = 2.7e-34
Identity = 92/240 (38.33%), Postives = 117/240 (48.75%), Query Frame = 0

Query: 19  LLLLLPSTFNAHPLKACMFDAIYQLGDSISDTGNLIRENPATPFSHLPYGETFFKNATGR 78
           LL+ +      H    C  ++IYQ GDSISDTGNLIR  PA+  +  P            
Sbjct: 9   LLVFILFVSLVHSSDQCPINSIYQFGDSISDTGNLIRNGPASSPTPKP------------ 68

Query: 79  CSNGLLMIDHFVHCNNQYALSFCSFG--GWTSSDQPVFEQRGIDESWGEFCSGWLYGFAF 138
               L   +H V         F +FG  G T+ +   F +R                   
Sbjct: 69  ----LPQREHNV---------FVNFGVSGSTALNSSFFSER------------------- 128

Query: 139 GSSCSKEISSPVTNSSLTKQLDWMSAHFNTTC-SHQRDCTEKLKNALFLVGEIGGNDYNY 198
                  +  P TN+ L+ QL W   H  +TC     DC   LK++LF+VGEIGGNDYNY
Sbjct: 129 ------NLHVPATNTPLSMQLAWFKGHLRSTCHGSSSDC---LKHSLFMVGEIGGNDYNY 188

Query: 199 ALFQGKTVEEVRNMVPEVVQTIKDAVTRVISYGAARVLVPGNFPIGCLPIYLTGFQTKDS 256
             FQGK +EE+R+ +P VV  I  A   VI  GA  V+VPGNFP+GC PIYLT F  KD+
Sbjct: 189 GFFQGKPMEEIRSYIPHVVGAITAAAREVIRAGAVNVVVPGNFPVGCFPIYLTSFPVKDT 195

BLAST of Sgr023071 vs. ExPASy Swiss-Prot
Match: Q8RXT9 (GDSL esterase/lipase At1g28590 OS=Arabidopsis thaliana OX=3702 GN=At1g28590 PE=2 SV=2)

HSP 1 Score: 145.2 bits (365), Expect = 1.0e-33
Identity = 91/237 (38.40%), Postives = 120/237 (50.63%), Query Frame = 0

Query: 37  FDAIYQLGDSISDTGNLI-----RENPATPFSHLPYGETFFKNATGRCSNGLLMIDH--- 96
           F +I   GDSI+DTGNL+      + PA+ F   PYGETFF + TGR S+G L+ID    
Sbjct: 34  FKSIISFGDSIADTGNLLGLSDPNDLPASAFP--PYGETFFHHPTGRYSDGRLIIDFIAE 93

Query: 97  ---------FVHCNNQYALSFCSF--GGWTSSDQPVFEQRGIDESWGEFCSGWLYGFAFG 156
                    F  C N       +F   G T+ +    E+RG                   
Sbjct: 94  FLGFPLVPPFYGCQNANFKKGVNFAVAGATALEPSFLEERG------------------- 153

Query: 157 SSCSKEISSPVTNSSLTKQLDWMSAHFNTTCSHQRDCTEKLKNALFLVGEIGGNDYNYAL 216
                 I S +TN SL+ QL   +      C    DC + ++NAL L+GEIGGNDYN+AL
Sbjct: 154 ------IHSTITNVSLSVQLRSFTESLPNLCGSPSDCRDMIENALILMGEIGGNDYNFAL 213

Query: 217 FQGKTVEEVRNMVPEVVQTIKDAVTRVISYGAARVLVPGNFPIGCLPIYLTGFQTKD 255
           FQ K V+EV  +VP V+ TI  A+T ++  G    LVPGNFPIG    YLT ++T +
Sbjct: 214 FQRKPVKEVEELVPFVIATISSAITELVCMGGRTFLVPGNFPIGYSASYLTLYKTSN 243

BLAST of Sgr023071 vs. ExPASy Swiss-Prot
Match: Q9FXJ2 (GDSL esterase/lipase At1g28580 OS=Arabidopsis thaliana OX=3702 GN=At1g28580 PE=2 SV=1)

HSP 1 Score: 144.8 bits (364), Expect = 1.4e-33
Identity = 92/266 (34.59%), Postives = 130/266 (48.87%), Query Frame = 0

Query: 7   SVLALALLCSSFLLLLLPSTFNAHPLKACMFDAIYQLGDSISDTGNLIRENPATPFSHL- 66
           S + + LL   FL   + +  ++   K   F +I   GDSI+DTGNL+  +      H+ 
Sbjct: 6   SPILMKLLVFIFLSTFVVTNVSSE-TKCREFKSIISFGDSIADTGNLLGLSDPKDLPHMA 65

Query: 67  --PYGETFFKNATGRCSNGLLMIDHFV---------------HCNNQYALSFCSFGGWTS 126
             PYGE FF + TGR SNG L+ID                  + N +  ++F + GG T+
Sbjct: 66  FPPYGENFFHHPTGRFSNGRLIIDFIAEFLGLPLVPPFYGSHNANFEKGVNF-AVGGATA 125

Query: 127 SDQPVFEQRGIDESWGEFCSGWLYGFAFGSSCSKEISSPVTNSSLTKQLDWMSAHFNTTC 186
            ++   E RG                         I  P TN SL  QL+       + C
Sbjct: 126 LERSFLEDRG-------------------------IHFPYTNVSLGVQLNSFKESLPSIC 185

Query: 187 SHQRDCTEKLKNALFLVGEIGGNDYNYALFQGKTVEEVRNMVPEVVQTIKDAVTRVISYG 246
               DC + ++NAL L+GEIGGNDYNYA F  K +EE++ ++P V+ TI  A+T +I  G
Sbjct: 186 GSPSDCRDMIENALILMGEIGGNDYNYAFFVDKGIEEIKELMPLVITTISSAITELIGMG 244

Query: 247 AARVLVPGNFPIGCLPIYLTGFQTKD 255
               LVPG FP+GC  +YLT  QT +
Sbjct: 246 GRTFLVPGEFPVGCSVLYLTSHQTSN 244

BLAST of Sgr023071 vs. ExPASy TrEMBL
Match: A0A6J1CSC4 (acetylajmalan esterase-like OS=Momordica charantia OX=3673 GN=LOC111013753 PE=3 SV=1)

HSP 1 Score: 303.1 bits (775), Expect = 1.1e-78
Identity = 166/254 (65.35%), Postives = 181/254 (71.26%), Query Frame = 0

Query: 8   VLALALLCSSFLLLLLPSTFNAHPLKACMFDAIYQLGDSISDTGNLIRENPATPFSHLPY 67
           +L+  LLCSS   LLLPS  NAH LKAC FDAIYQLGDSISDTGNLIRENP TPFSHLPY
Sbjct: 2   MLSFLLLCSS---LLLPSPSNAHLLKACKFDAIYQLGDSISDTGNLIRENPVTPFSHLPY 61

Query: 68  GETFFKNATGRCSNGLLMIDHFVHCNNQYALSFCSFGGWTSSDQPVFEQRGIDESWGEFC 127
           GETFFKNATGRCSNGLLMID F       A        + + D               F 
Sbjct: 62  GETFFKNATGRCSNGLLMIDQFA-----LAAGLPLINPYLNKD-------------ASFT 121

Query: 128 SGWLYGFAFGSSCS------KEISSPVTNSSLTKQLDWMSAHFNTTCSHQRDCTEKLKNA 187
            G  +  A  ++ S      K I SP TNSSLTKQLDWMS HF+T CSH +     L +A
Sbjct: 122 HGVNFAVAGSTALSSQVLAQKMILSPATNSSLTKQLDWMSTHFDTICSHHKGL--YLNDA 181

Query: 188 LFLVGEIGGNDYNYALFQGKTVEEVRNMVPEVVQTIKDAVTRVISYGAARVLVPGNFPIG 247
           LFLVGEIGGNDYNYALFQGKT+EEV+NMVPEVVQTIKDA+ +VISYGA RV+VPGNFPIG
Sbjct: 182 LFLVGEIGGNDYNYALFQGKTIEEVKNMVPEVVQTIKDAIIKVISYGAVRVIVPGNFPIG 232

Query: 248 CLPIYLTGFQTKDS 256
           CLPIYLTGFQTK S
Sbjct: 242 CLPIYLTGFQTKIS 232

BLAST of Sgr023071 vs. ExPASy TrEMBL
Match: A0A6J1DQL3 (acetylajmalan esterase-like OS=Momordica charantia OX=3673 GN=LOC111023320 PE=3 SV=1)

HSP 1 Score: 293.5 bits (750), Expect = 8.7e-76
Identity = 151/253 (59.68%), Postives = 178/253 (70.36%), Query Frame = 0

Query: 9   LALALLCSSF------LLLLLPSTFNAHPLKACMFDAIYQLGDSISDTGNLIRENPATPF 68
           +A++   SSF      L LLL S   AHPL+ACMFDAIYQLGDSISDTGNLIRENP T F
Sbjct: 1   MAISFTTSSFSLLLPLLFLLLSSASTAHPLRACMFDAIYQLGDSISDTGNLIRENPNTTF 60

Query: 69  SHLPYGETFFKNATGRCSNGLLMIDHFVHCNNQYALSFCSFGGWTSSDQPVFEQRGIDES 128
           SHLPYGETFF  ATGRCSNGLLM+D+F            +         P   +  +   
Sbjct: 61  SHLPYGETFFHGATGRCSNGLLMLDYF------------ALDAGLPLVNPYLNRDALTNH 120

Query: 129 WGEFCSGWLYGFAFGSSCSKEISSPVTNSSLTKQLDWMSAHFNTTCSHQRDCTEKLKNAL 188
              F        +F +    +I SPVTNSSL  QL WM +HF + C ++RDC EKL++AL
Sbjct: 121 GVNFAVAGSTALSFEALLRNKILSPVTNSSLDHQLGWMHSHFESICLNERDCIEKLRSAL 180

Query: 189 FLVGEIGGNDYNYALFQGKTVEEVRNMVPEVVQTIKDAVTRVISYGAARVLVPGNFPIGC 248
           FLVGEIGGNDYNYALFQGKT++EV++MVP+VVQTIK+AV +VISYGA RV+VPGNFPIGC
Sbjct: 181 FLVGEIGGNDYNYALFQGKTIQEVKDMVPDVVQTIKNAVEKVISYGATRVIVPGNFPIGC 240

Query: 249 LPIYLTGFQTKDS 256
           LPIYLTGFQT D+
Sbjct: 241 LPIYLTGFQTNDT 241

BLAST of Sgr023071 vs. ExPASy TrEMBL
Match: A0A2N9H015 (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS33204 PE=3 SV=1)

HSP 1 Score: 293.1 bits (749), Expect = 1.1e-75
Identity = 157/252 (62.30%), Postives = 177/252 (70.24%), Query Frame = 0

Query: 4   STKSVLALALLCSSFLLLLLPSTFNAHPLKACMFDAIYQLGDSISDTGNLIRENPATPFS 63
           +T SVL+L L   +F LLL P    AHPLK CMFDAIYQLGDSISDTGNLIRENPA  F+
Sbjct: 3   TTNSVLSLLLTLIAFSLLLFPRCIGAHPLKVCMFDAIYQLGDSISDTGNLIRENPAVAFA 62

Query: 64  HLPYGETFFKNATGRCSNGLLMIDHFVHCNNQYALSFCSFGGWTSSDQPVFEQRGIDESW 123
            LPYGET FK ATGRCSNGLLMID+         + F S       D+     RG  E++
Sbjct: 63  RLPYGETSFKKATGRCSNGLLMIDYIA---LSAGIPFLS----PYLDKDALFVRGCGENF 122

Query: 124 GEFCSGWLYGFAFGSSCSKEISSPVTNSSLTKQLDWMSAHFNTTCSHQRDCTEKLKNALF 183
               S  L          K ISSPVTNSSL  QLDWMS HFN  C +  DC +KL+ ALF
Sbjct: 123 AVAGSTALPAQIL---AEKNISSPVTNSSLNVQLDWMSTHFNDICYNADDCIKKLQTALF 182

Query: 184 LVGEIGGNDYNYALFQGKTVEEVRNMVPEVVQTIKDAVTRVISYGAARVLVPGNFPIGCL 243
           +VGE+G NDYNYALFQGKT+E+VRNMVPEVVQ IK+AVTRVI +GAARV+VPGNFPIGC 
Sbjct: 183 MVGEVGWNDYNYALFQGKTIEDVRNMVPEVVQAIKEAVTRVIGFGAARVVVPGNFPIGCS 242

Query: 244 PIYLTGFQTKDS 256
           PIYLT FQT +S
Sbjct: 243 PIYLTAFQTNNS 244

BLAST of Sgr023071 vs. ExPASy TrEMBL
Match: A0A6J1G523 (acetylajmalan esterase-like OS=Cucurbita moschata OX=3662 GN=LOC111450823 PE=3 SV=1)

HSP 1 Score: 291.2 bits (744), Expect = 4.3e-75
Identity = 155/257 (60.31%), Postives = 178/257 (69.26%), Query Frame = 0

Query: 1   MAVSTKSVLALALLCSSFLLLLL--PSTFNAHPLKACMFDAIYQLGDSISDTGNLIRENP 60
           MA STKS  ++ LL    LL +L  PS+  AH LK CMFDAIYQLGDSISDTGNLIR+NP
Sbjct: 1   MAASTKSPSSIVLLVLLLLLSVLGCPSS-KAHLLKTCMFDAIYQLGDSISDTGNLIRQNP 60

Query: 61  ATPFSHLPYGETFFKNATGRCSNGLLMIDHFVHCNNQYALSFCSFGGWTSSDQPVFEQRG 120
            TPFSHLPYGETFF  +TGRCSNGLLMID+F            +         P   +  
Sbjct: 61  NTPFSHLPYGETFFNKSTGRCSNGLLMIDYF------------ALDAGLPLVNPYLNKDA 120

Query: 121 IDESWGEFCSGWLYGFAFGSSCSKEISSPVTNSSLTKQLDWMSAHFNTTCSHQRDCTEKL 180
           +      F        +       +I SPVTNSSL +QL WM +HFN+ C  QRDC EKL
Sbjct: 121 LTRHGVNFAVAGSTALSSQLLSQNQILSPVTNSSLNQQLYWMFSHFNSICYDQRDCNEKL 180

Query: 181 KNALFLVGEIGGNDYNYALFQGKTVEEVRNMVPEVVQTIKDAVTRVISYGAARVLVPGNF 240
           +NALF VGEIGGNDYNYALFQGKT+ EV++MVP+VVQTIK AV +VISYGA RV+VPGNF
Sbjct: 181 RNALFFVGEIGGNDYNYALFQGKTIPEVKDMVPQVVQTIKSAVEKVISYGATRVVVPGNF 240

Query: 241 PIGCLPIYLTGFQTKDS 256
           PIGCLPIYLTGFQT D+
Sbjct: 241 PIGCLPIYLTGFQTNDT 244

BLAST of Sgr023071 vs. ExPASy TrEMBL
Match: A0A6J1KFS3 (acetylajmalan esterase-like OS=Cucurbita maxima OX=3661 GN=LOC111493492 PE=3 SV=1)

HSP 1 Score: 288.9 bits (738), Expect = 2.2e-74
Identity = 153/257 (59.53%), Postives = 181/257 (70.43%), Query Frame = 0

Query: 1   MAVSTK--SVLALALLCSSFLLLLLPSTFNAHPLKACMFDAIYQLGDSISDTGNLIRENP 60
           MA STK  S + LA+L     ++  PS+  AH LK CMFDAIYQLGDSISDTGNLIR+NP
Sbjct: 1   MAPSTKSPSSIVLAVLLVLVSVIGCPSS-TAHLLKTCMFDAIYQLGDSISDTGNLIRQNP 60

Query: 61  ATPFSHLPYGETFFKNATGRCSNGLLMIDHFVHCNNQYALSFCSFGGWTSSDQPVFEQRG 120
            TPFSHLPYGET F  +TGRCSNGLLMID+F            +         P   +  
Sbjct: 61  NTPFSHLPYGETLFNKSTGRCSNGLLMIDYF------------ALDAGLPLVNPYLNKDA 120

Query: 121 IDESWGEFCSGWLYGFAFGSSCSKEISSPVTNSSLTKQLDWMSAHFNTTCSHQRDCTEKL 180
           +      F        +      K+I SPVTNSSL +QL WM +HFN+ C +QRDC EKL
Sbjct: 121 LTRHGVNFAVAGSTALSSQLLSQKQILSPVTNSSLNQQLYWMFSHFNSICYNQRDCNEKL 180

Query: 181 KNALFLVGEIGGNDYNYALFQGKTVEEVRNMVPEVVQTIKDAVTRVISYGAARVLVPGNF 240
           +NALFLVGEIGGNDYNYALFQGKT+++V++MVP+VVQTIK+AV +VISYGA RV+VPGNF
Sbjct: 181 RNALFLVGEIGGNDYNYALFQGKTIQDVKDMVPQVVQTIKNAVEKVISYGATRVVVPGNF 240

Query: 241 PIGCLPIYLTGFQTKDS 256
           PIGCLPIYLTGFQT D+
Sbjct: 241 PIGCLPIYLTGFQTNDT 244

BLAST of Sgr023071 vs. TAIR 10
Match: AT1G28570.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 153.3 bits (386), Expect = 2.7e-37
Identity = 97/272 (35.66%), Postives = 138/272 (50.74%), Query Frame = 0

Query: 1   MAVSTKSVLALALLCSSFLLLLLPSTFNAHPLKACMFDAIYQLGDSISDTGNLIRENPAT 60
           MA     +++  L+ S+F L    +T N+ P +   F +I   GDSI+DTGNL+  +  T
Sbjct: 1   MATLFMKLVSFFLILSTFCL----TTVNSEP-QCHNFKSIISFGDSIADTGNLLALSDPT 60

Query: 61  ---PFSHLPYGETFFKNATGRCSNGLLMIDHFV---------------HCNNQYALSFCS 120
                + LPYGETFF + TGR SNG L+ID                  + N +  ++F +
Sbjct: 61  NLPKVAFLPYGETFFHHPTGRFSNGRLIIDFIAEFLGFPLVPPFYGSQNANFEKGVNF-A 120

Query: 121 FGGWTSSDQPVFEQRGIDESWGEFCSGWLYGFAFGSSCSKEISSPVTNSSLTKQLDWMSA 180
            GG T+ ++   E+RG                         I  P TN SL  QL     
Sbjct: 121 VGGATALERSFLEERG-------------------------IHFPYTNVSLAVQLSSFKE 180

Query: 181 HFNTTCSHQRDCTEKLKNALFLVGEIGGNDYNYALFQGKTVEEVRNMVPEVVQTIKDAVT 240
                C    DC + ++N+L L+GEIGGNDYNYA F GK +EE++ +VP V++TI  A+T
Sbjct: 181 SLPNLCVSPSDCRDMIENSLILMGEIGGNDYNYAFFVGKNIEEIKELVPLVIETISSAIT 240

Query: 241 RVISYGAARVLVPGNFPIGCLPIYLTGFQTKD 255
            +I  G    LVPG FP+GC   YL+ +QT +
Sbjct: 241 ELIGMGGKTFLVPGEFPLGCSVAYLSLYQTSN 241

BLAST of Sgr023071 vs. TAIR 10
Match: AT5G03980.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 147.1 bits (370), Expect = 1.9e-35
Identity = 92/240 (38.33%), Postives = 117/240 (48.75%), Query Frame = 0

Query: 19  LLLLLPSTFNAHPLKACMFDAIYQLGDSISDTGNLIRENPATPFSHLPYGETFFKNATGR 78
           LL+ +      H    C  ++IYQ GDSISDTGNLIR  PA+  +  P            
Sbjct: 9   LLVFILFVSLVHSSDQCPINSIYQFGDSISDTGNLIRNGPASSPTPKP------------ 68

Query: 79  CSNGLLMIDHFVHCNNQYALSFCSFG--GWTSSDQPVFEQRGIDESWGEFCSGWLYGFAF 138
               L   +H V         F +FG  G T+ +   F +R                   
Sbjct: 69  ----LPQREHNV---------FVNFGVSGSTALNSSFFSER------------------- 128

Query: 139 GSSCSKEISSPVTNSSLTKQLDWMSAHFNTTC-SHQRDCTEKLKNALFLVGEIGGNDYNY 198
                  +  P TN+ L+ QL W   H  +TC     DC   LK++LF+VGEIGGNDYNY
Sbjct: 129 ------NLHVPATNTPLSMQLAWFKGHLRSTCHGSSSDC---LKHSLFMVGEIGGNDYNY 188

Query: 199 ALFQGKTVEEVRNMVPEVVQTIKDAVTRVISYGAARVLVPGNFPIGCLPIYLTGFQTKDS 256
             FQGK +EE+R+ +P VV  I  A   VI  GA  V+VPGNFP+GC PIYLT F  KD+
Sbjct: 189 GFFQGKPMEEIRSYIPHVVGAITAAAREVIRAGAVNVVVPGNFPVGCFPIYLTSFPVKDT 195

BLAST of Sgr023071 vs. TAIR 10
Match: AT1G28590.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 145.2 bits (365), Expect = 7.4e-35
Identity = 91/237 (38.40%), Postives = 120/237 (50.63%), Query Frame = 0

Query: 37  FDAIYQLGDSISDTGNLI-----RENPATPFSHLPYGETFFKNATGRCSNGLLMIDH--- 96
           F +I   GDSI+DTGNL+      + PA+ F   PYGETFF + TGR S+G L+ID    
Sbjct: 34  FKSIISFGDSIADTGNLLGLSDPNDLPASAFP--PYGETFFHHPTGRYSDGRLIIDFIAE 93

Query: 97  ---------FVHCNNQYALSFCSF--GGWTSSDQPVFEQRGIDESWGEFCSGWLYGFAFG 156
                    F  C N       +F   G T+ +    E+RG                   
Sbjct: 94  FLGFPLVPPFYGCQNANFKKGVNFAVAGATALEPSFLEERG------------------- 153

Query: 157 SSCSKEISSPVTNSSLTKQLDWMSAHFNTTCSHQRDCTEKLKNALFLVGEIGGNDYNYAL 216
                 I S +TN SL+ QL   +      C    DC + ++NAL L+GEIGGNDYN+AL
Sbjct: 154 ------IHSTITNVSLSVQLRSFTESLPNLCGSPSDCRDMIENALILMGEIGGNDYNFAL 213

Query: 217 FQGKTVEEVRNMVPEVVQTIKDAVTRVISYGAARVLVPGNFPIGCLPIYLTGFQTKD 255
           FQ K V+EV  +VP V+ TI  A+T ++  G    LVPGNFPIG    YLT ++T +
Sbjct: 214 FQRKPVKEVEELVPFVIATISSAITELVCMGGRTFLVPGNFPIGYSASYLTLYKTSN 243

BLAST of Sgr023071 vs. TAIR 10
Match: AT1G28580.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 144.8 bits (364), Expect = 9.7e-35
Identity = 92/266 (34.59%), Postives = 130/266 (48.87%), Query Frame = 0

Query: 7   SVLALALLCSSFLLLLLPSTFNAHPLKACMFDAIYQLGDSISDTGNLIRENPATPFSHL- 66
           S + + LL   FL   + +  ++   K   F +I   GDSI+DTGNL+  +      H+ 
Sbjct: 6   SPILMKLLVFIFLSTFVVTNVSSE-TKCREFKSIISFGDSIADTGNLLGLSDPKDLPHMA 65

Query: 67  --PYGETFFKNATGRCSNGLLMIDHFV---------------HCNNQYALSFCSFGGWTS 126
             PYGE FF + TGR SNG L+ID                  + N +  ++F + GG T+
Sbjct: 66  FPPYGENFFHHPTGRFSNGRLIIDFIAEFLGLPLVPPFYGSHNANFEKGVNF-AVGGATA 125

Query: 127 SDQPVFEQRGIDESWGEFCSGWLYGFAFGSSCSKEISSPVTNSSLTKQLDWMSAHFNTTC 186
            ++   E RG                         I  P TN SL  QL+       + C
Sbjct: 126 LERSFLEDRG-------------------------IHFPYTNVSLGVQLNSFKESLPSIC 185

Query: 187 SHQRDCTEKLKNALFLVGEIGGNDYNYALFQGKTVEEVRNMVPEVVQTIKDAVTRVISYG 246
               DC + ++NAL L+GEIGGNDYNYA F  K +EE++ ++P V+ TI  A+T +I  G
Sbjct: 186 GSPSDCRDMIENALILMGEIGGNDYNYAFFVDKGIEEIKELMPLVITTISSAITELIGMG 244

Query: 247 AARVLVPGNFPIGCLPIYLTGFQTKD 255
               LVPG FP+GC  +YLT  QT +
Sbjct: 246 GRTFLVPGEFPVGCSVLYLTSHQTSN 244

BLAST of Sgr023071 vs. TAIR 10
Match: AT1G28670.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 142.5 bits (358), Expect = 4.8e-34
Identity = 100/256 (39.06%), Postives = 130/256 (50.78%), Query Frame = 0

Query: 10  ALALLCSSFLLLLLPSTF--NAHPLKACMFDAIYQLGDSISDTGNLIR---ENPATPFSH 69
           +L  L SSFLL+L  +T    +   +   F +I   GDSI+DTGN +     N     + 
Sbjct: 4   SLKKLISSFLLVLYSTTIIVASSESRCRRFKSIISFGDSIADTGNYLHLSDVNHLPQSAF 63

Query: 70  LPYGETFFKNATGRCSNGLLMIDHFVHCNNQYALSFCSFGGWTSSDQPVFEQRGIDESWG 129
           LPYGE+FF   +GR SNG L+ID          L       +  S    FEQ GI+ +  
Sbjct: 64  LPYGESFFHPPSGRASNGRLIIDFIAE-----FLGLPYVPPYFGSQNVSFEQ-GINFA-- 123

Query: 130 EFCSGWLYG-------FAFGSSCSKEISSPVTNSSLTKQLDWMSAHFNTTC-SHQRDCTE 189
                 +YG       F  G    K I S  TN SL+ QLD         C S  RDC E
Sbjct: 124 ------VYGATALDRAFLLG----KGIESDFTNVSLSVQLDTFKQILPNLCASSTRDCKE 183

Query: 190 KLKNALFLVGEIGGNDYNYALFQGKTVEEVRNMVPEVVQTIKDAVTRVISYGAARVLVPG 249
            L ++L L+GEIGGNDYNY  F+GK++ E++ +VP +V+ I  A+  +I  G    LVPG
Sbjct: 184 MLGDSLILMGEIGGNDYNYPFFEGKSINEIKELVPLIVKAISSAIVDLIDLGGKTFLVPG 241

Query: 250 NFPIGCLPIYLTGFQT 253
            FP GC   YLT FQT
Sbjct: 244 GFPTGCSAAYLTLFQT 241