Sgr021206 (gene) Monk fruit (Qingpiguo) v1

Overview
NameSgr021206
Typegene
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Description4-hydroxy-3-methylbut-2-enyl diphosphate reductase
Locationtig00153653: 89685 .. 95126 (+)
RNA-Seq ExpressionSgr021206
SyntenySgr021206
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATCTCCCGCCTTCCCCATCGCACAGACTTCGTTTCACCGAAGATCTTCGCCGCCGGAGCTCTGCGCGCCGGAAACTTCTTTCCCTCCGGTGCACTGCCGATTCTTCGTCTTCTCCGGAGCCGGTCGATCCGATTTCGATGCGAAGTTGTTCCGGAAGAACTTGGCCAGAGGTAGAAACTACAATCGGAAAGGGTTTGGGCACAAGGAGGAGACGATTAAGCTCATGAGCAGCGAGTTCACGAGTGAGTGTTCTTGCATTTGATGATACGAACATCATTACGATTTTCAGGGCTGTATTATTGTCTCTTGGTGCCGATTTTGTATTGAATGGCTCAGGTGAAGTTATAAAAACTCTGAATGAAAATGGGAATGAGTTCACATGGGGAATGTTACTGTGAAATTGGCTGGTGCTTATGGGTTTTGCTGGGGAGTTGAGCGAGCTGTGCAGATTGCTTATGAAGCCAGGAAACAATTCCCAAATGGAACGATTTGGATTACCAATGAAATTATTCACAACCCAACTGTTAACAAGGTCCTTCAACTTTTCTTGCCTTTACTATTCCATTAGGTAGATTACCATGAAAGTTCTATTAACATACTACTGTTTATATTTGGCTTACCATCTATAGTTTAAAACCTATTTTGATCTGTTTGGGTCCCCAAACTTCCAAATATTTTGTTTTGGTCCCTAAGTTCATTAATGATTTTATATAAATCTGAATCTATTATGTAGACATTTACTCATGCCTATGTTAGATTTCTGTTGAATAGTTTACAAAAAAAAACTATTGGCAAGAATTAAAACGGTTTTTTTATATAAAGTACAGAAACTAACAGGAGGTTAAAGTTTAGGGACCGAAATGAATAGATATGAAAGTTTAGTGAGACCCACACCTTTGTAGGTTAATGACATGGCTAATGAAAGAATGTCCATACTATGATAGCACTAGAAACTTACTCTTTCTTTTTGCGTTGTTTTCTTCTGTTCTGCTTTATGGTAGCCAACAACATGATATGTTATGCTTGAAAAGAAATATCATTTTGTAATTCTTTGAAAGATTCTTATGAGCACAGCGTTTGGAGGAAATGGGAGTGCATAACATTCCTCTTGAGGAAGGAAAGAAACGGTTTGATGTCGTTAATCCAGGCGATGTTGTGATTTTGCCTGCCTTTGGGGCTGCAGTTGATGAAATGCTTGTTTTGAATGACAAAAATGTTCAGATTGTTGACACAACTTGCCCATGGGTTTCCAAGGTACTCTGCCATTTTCTTGTCCTTCACAATTCCCTCCTCCAAGGACATCCTCTTATGCTGCCTTCTGTGAATGTTTTTCTTTTGGTCAATGTTCCACTTATTACCATTATTGAAGGAGAAAAGGCACATTGCAGCTGTTGATGCTGAGATTATACCGCACTTCATTCGTGTAGGTTTGGAACATTGTTGACAAGCACAAAAAAGGAGAATACACGTCAATAATTCATGGTAAATATCGTCATGAAGAAACCGTGGCAACGCTTCTTTCGCTGGAAAATACATTGTTGTAAAAGACATGGCAGAGGTACATCCTTGGTAGTTTTATCCCTTTTCTAACATGTCAAAACTTTTGGCACATAGCATAAGTAACCATTTTTAAAGGGTAAAAGTTTGATCCTAGTCAGGCAACATATGTAAGCAACTACATTCTCAGGGTTGAACATAACGGATCTAGCTCAACCAAAGAAGAATTTTTGAAGGTATGATCTTTTAGTTATTTGCTTAATCAAACTTGCTGATATATTTGACATCATGCACAGTATGAGTCAAATTTCGAGCTTGATTGCAGAAATTCAAGTCTGCTGTTTCAGAAGGATTTGATCCAGAAATTGACCTCGTAAAAGTCGGCATCGCCAATCAGACTACAATGCTCAAGGGGGAAACAGAAGAGATTGGTTAGTATTTGGAGGTGTTGATGCTTTGTCGAACCATCATTTCCGACAAAGCTGCGTTCTGTGGATTACTGATGTCATGATTTTGATAACAGGGAAATTGATAGAGAAGACTATGATGCGCAAGTATGGAGTTGAAAATATTAATGAGCACTTCATTAGTTTCAATACGATCTGCGAAGCTACTCAAGTAAGTACCGTCGTTCTTCTTGTAACGTATAGCTTATTGGAAAGGAAGCCTTCACAAAAATTCCCACCGACTAGATCAGCTCCTTTTCTTTTTAAGGGAAGTTTTCATTATGGATAGTTTAGCATTAGTAAATGTTAAGGGTGCGACTAAAGGCCTACGTTAGTTAGAAATGGGTAAGATGATTGGTTTATAAATAAGGATGAATTGATGTTACATCTTATTTGTCTAAGTTCAAAAGTAAATTCATATTGACTAGGTCAAAATAGACAATATCGCACTAATGAAATAGTGATTTACTTTACTTGCAAGAGAGTCTTGTGATGGTGATTGGAGGTGTTGTCTTCCTCAGGAGCGCCAAGATGCATGTACCAGCTGGTGGAGGAAGATATGGATCTCATGTTAGTTGTAGGTGGATGGAACTCAAGCAACACCTCTCATCTTCAAGAGATTGCCGAGACTCGAGGAATCCCGTCGTATTGGATCGACAGCGAGCAACGAATAGGTCCAGGAAACTCAATATCATACAAGCTCACTGTAAGTCTGAATTCAGAGTGTTAATCCAAAGATCCTACTTGCCATCTGTTCTTCTTTCCAACTTATATTTACTCGTTTATTCGTACGATAGCACGGGGAGCTGGCCAAGAAAGAAAACTGGCTACCTGATGGACATGTGACCATAGGAGTGACCTCCGGTGCCTCCACACCAGATAAGGTAAGTGAAAAGTTACTGTCAAACCTTGGAATGTTGTATGAGACTGCAAACTGTTAAATCTTCCATTTTCCTTGACATTAACTTTTGTCAAATGGGTTAAAATTTTAGGTTGTTGAAAATGTTCTTAAGAAGGTTTTTGACATCAAAGGGGAAGGAACTGTGCAGCTTGCATAGCTTGGATAAAAGCAAACTACAGTGTATAATGTTACTAAACTGTTTTTTTTTTTTGGTTGGTCTTGTTATATGGTATGGTTTGGTAGGATGTTTGCTAGATTATATGTAATATATTAAAAATAGTGACAAAATAAGTAGACTTGCAACATTATCATAAAAATAATCTCAGCAGATGCATGTCAAAGGAAATATCCTGGAAAGGGAAAGACGAAATCGGTAGAAATTTTTAAAAAAAAAGAGAGACAGAAGAGAATATGGTAATATTCTACTTCTTTTTTTTTTTTTTTCCTGTTTCTTATTTTTTAAAGAAGAAAAACAAAATCGTATTCGTTAATTGCTTCTTGTTGTTGTTGGCTTTTTTTTTAATTGAAAAAAGATTTTTTTTTTCTCGTTCTACAATTTTTAAATTGAAAAAATTGAATTTAAATTATAAATTATCTTATAGTCCCAAAACTTAAAAAAAATTAAATTAGGTTCTTAAACATTTGATTTGATTCTAACAAGTTTCTATTGTTAATGTAATTACATAAATGTTGAAGTTACATGTTAATTGTATTAATTTGATGTGATGTACGCCAAAAAAAAAGTCCTATTGCATATAATAAGAGCGTGTGAGGCAAAAAAAATTCAATGAGTAAGTAGACATTATTGCTTTAAAATATATAAATAAATATATCGTAAGTATTTCAAGGAATTTTAATAATGTATTGTGTAAGTCTTGATTTCTTTAGAGAGTTTTTGTCTAACTTGAGTTTAAGTATCTCTATTGTAAGTTCGAATCTTCGTTTCAATATTTGTAATATGATTTTTTAAATTAATTAAATAAATAAATAGAATATTAAGACTAGAGGTTAAAAGAGAGAGAAATAAACATTATTCATATGAAGGGGAATAAAAACTAAAGATCTACCGATCTAAAATCTAAACATATGAGAAAAGAAAACTAAAGAAGAAAATTGTCAGAGATCAGATATCATTGCATCAATTTTCCATCACATTTTCTTTCCTACCAAACAAACAAACAGAAAAAAAAAAAAAAAAAAACAATCCAATTTTCTTTCCGAACGAATTAAAAACAATACAAATGAAAGAATCTGATTACATAGTACCTACCAGTCGAAGTTTAGTACTCAGTTAAGGATGTTTTATGATTCTCTGCAAAACCACAGTCCTCGCCCTCGTAATGTCTCGGCTACACATATCAGCTTGAGCCCCCAAATCCTCTACGCTTTTCATGAACACCGCCAGTTTCTTCTTCATCTCCTCCACCCCAACCTTCACAGCTTCCTCTTTAATGGCGAAATCTGCCTTCTGCAACATCCCCTCGATCTCAATCTCCAGTTTCTCAATCAGAATCCGAATGTTGTCCATGTCTTTCATGGCACGTACGTCCCCACTTGCATGGACTTATCACTTCCTTGTGGCCTTTCAGTTCATTCTCGTAGCTCTTCCACAGAGAATCGATCCACTTCCCCATGGAGCCGAGGGGTATGGAGGACGCTGCAGATAGCGCGCCGCGACCGGCGGAGCGGCCATGACTGTCGCCACCACCGAGCAGATCAACACGGCGGCGAAGGTGGCGACGAATATCATGCTCGACAGCTTCCTCCATGTGCGGATGGATTTGAGCTTCTTGTCGAGTTTGTTTTTTCTCAGCTGCAGTTTCTCGAGCATTATCGTTTGCTGTTTGTAAACGGAGTGGAAGATTTTGAAGAACTCCTCGGTGAAAGGGTGGCCGGAGGCCTTGAAATTCCTCAGTTCTTGCAGAGTTTTCGCAAACTGATTCTGACCCACTTGAGTTTCTTCCTCCTCGAACTGCTGGAGCGCCATTAAAATGAGTAATTGGCTGTCACGAGCTCGCTTCAGGCACTTTTCCAAAGCAGTACAGAAATCGAGCGTCTGCAAACTGTTCTCGAAATACTCTTCGACGAGCTCGAACAGCTCCTGATTTTCCAGATGTCCTTTTTGCATTGCAATATAACTCTGACGACTTCTTGGTTCATTTCGAGCAAGCATTCTGTAACTTGCTTGAGAGAATCGAAGGAGAGGGCTTTGACTTCAACGCGACGGCCAAGCTGGTGATGACTTGGTTGGTGCGGGCATGGAGGTTCTCGTCGAAGGATCGGACGTCGACGTCGGCCCTGCAGGCTGCCGCGTAGGAGTTGAGCTCTTTGGCGTCGAGATTGATTATTGCAGACGACGGCGGTTCGGCGGCGCCCTTTTTGCTGGCGTGGTTTCCCATGGCGGCTGTTTGGATTCTGGACAAACCCAGATGGATTTTCTGTGAAAAGAGAAGAAAGAGCAGAAACCCAGTTTCAGAAAGACGACGATCTCTCGACTGGATCGAGAAGAAGAAGAAGGAGGAGGAGGAAGAAGAATGTGAAAAGGAAAGGCGGAGAGGGAAACTTGAAGTGAAAGAGAGAGGAAACAAAGATTGA

mRNA sequence

ATGATCTCCCGCCTTCCCCATCGCACAGACTTCGTTTCACCGAAGATCTTCGCCGCCGGAGCTCTGCGCGCCGGAAACTTCTTTCCCTCCGGTGCACTGCCGATTCTTCGTCTTCTCCGGAGCCGGTCGATCCGATTTCGATGCGAAGTTGTTCCGGAAGAACTTGGCCAGAGGGCTGTATTATTGTCTCTTGGTGCCGATTTTGTATTGAATGGCTCAGTTGAGCGAGCTGTGCAGATTGCTTATGAAGCCAGGAAACAATTCCCAAATGGAACGATTTGGATTACCAATGAAATTATTCACAACCCAACTGTTAACAAGCGTTTGGAGGAAATGGGAGTGCATAACATTCCTCTTGAGGAAGGAAAGAAACGGTTTGATGTCGTTAATCCAGGCGATGTTGTGATTTTGCCTGCCTTTGGGGCTGCAGTTGATGAAATGCTTGTTTTGAATGACAAAAATGTTCAGATTGTTGACACAACTTGCCCATGGGTTTCCAAGGAGAAAAGGCACATTGCAGCTGTTGATGCTGAGATTATACCGCACTTCATTCGTGTAGAAGGATTTGATCCAGAAATTGACCTCGTAAAAGTCGGCATCGCCAATCAGACTACAATGCTCAAGGGGGAAACAGAAGAGATTGGGAAATTGATAGAGAAGACTATGATGCGCAAGTATGGAGTTGAAAATATTAATGAGCACTTCATTAGTTTCAATACGATCTGCGAAGCTACTCAAGTGTTGTCTTCCTCAGGAGCGCCAAGATGCATGTACCAGCTGGTGGAGGAAGATATGGATCTCATGTTAGTTGTAGGTGGATGGAACTCAAGCAACACCTCTCATCTTCAAGAGATTGCCGAGACTCGAGGAATCCCGTCGTATTGGATCGACAGCGAGCAACGAATAGGTCCAGGAAACTCAATATCATACAAGCTCACTCACGGGGAGCTGGCCAAGAAAGAAAACTGGCTACCTGATGGACATGTGACCATAGGAGTGACCTCCGGTGCCTCCACACCAGATAAGTCGAAGTTTAGTACTCAGTTAAGGATGTTTTATGATTCTCTGCAAAACCACAGTCCTCGCCCTCGTAATGTCTCGGCTACACATATCAGCTTGAGCCCCCAAATCCTCTACGCTTTTCATGAACACCGCCAGTTTCTTCTTCATCTCCTCCACCCCAACCTTCACAGCTTCCTCTTTAATGGCGAAATCTGCCTTCTGCAACATCCCCTCGATCTCAATCTCCAGTTTCTCAATCAGAATCCGAATGTTGTCCATGTCTTTCATGGCACGTACGTCCCCACTTGCATGGACTTATCACTTCCTTGTGGCCTTTCAGTTCATTCTCGTAGCTCTTCCACAGAGAATCGATCCACTTCCCCATGGAGCCGAGGGGTATGGAGGACGCTGCAGATAGCGCGCCGCGACCGGCGGAGCGGCCATGACTGTCGCCACCACCGAGCAGATCAACACGGCGGCGAAGGTGGCGACGAATATCATGCTCGACAGCTTCCTCCATGTGCGGATGGATTTGAGCTTCTTGTCGAGTTTGTTTTTTCTCAGCTGCAGTTTCTCGAGCATTATCGTTTGCTGTTTGTAAACGGAGTGGAAGATTTTGAAGAACTCCTCGGTGAAAGGGTGGCCGGAGGCCTTGAAATTCCTCAGTTCTTGCAGAGCACTTTTCCAAAGCAGTACAGAAATCGAGCGTCTGCAAACTGTTCTCGAAATACTCTTCGACGAGCTCGAACAGCTCCTGATTTTCCAGATGTCCTTTTTGCATTGCAATATAACTCTGACGACTTCTTGGTTCATTTCGAGCAAGCATTCTGTAACTTGCTTGAGAGAATCGAAGGAGAGGGCTTTGACTTCAACGCGACGGCCAAGCTGGTGATGACTTGGTTGGTGCGGGCATGGAGGTTCTCGTCGAAGGATCGGACGTCGACGTCGGCCCTGCAGGCTGCCGCATGGATTTTCTGTGAAAAGAGAAGAAAGAGCAGAAACCCAGTTTCAGAAAGACGACGATCTCTCGACTGGATCGAGAAGAAGAAGAAGGAGGAGGAGGAAGAAGAATGTGAAAAGGAAAGGCGGAGAGGGAAACTTGAAGTGAAAGAGAGAGGAAACAAAGATTGA

Coding sequence (CDS)

ATGATCTCCCGCCTTCCCCATCGCACAGACTTCGTTTCACCGAAGATCTTCGCCGCCGGAGCTCTGCGCGCCGGAAACTTCTTTCCCTCCGGTGCACTGCCGATTCTTCGTCTTCTCCGGAGCCGGTCGATCCGATTTCGATGCGAAGTTGTTCCGGAAGAACTTGGCCAGAGGGCTGTATTATTGTCTCTTGGTGCCGATTTTGTATTGAATGGCTCAGTTGAGCGAGCTGTGCAGATTGCTTATGAAGCCAGGAAACAATTCCCAAATGGAACGATTTGGATTACCAATGAAATTATTCACAACCCAACTGTTAACAAGCGTTTGGAGGAAATGGGAGTGCATAACATTCCTCTTGAGGAAGGAAAGAAACGGTTTGATGTCGTTAATCCAGGCGATGTTGTGATTTTGCCTGCCTTTGGGGCTGCAGTTGATGAAATGCTTGTTTTGAATGACAAAAATGTTCAGATTGTTGACACAACTTGCCCATGGGTTTCCAAGGAGAAAAGGCACATTGCAGCTGTTGATGCTGAGATTATACCGCACTTCATTCGTGTAGAAGGATTTGATCCAGAAATTGACCTCGTAAAAGTCGGCATCGCCAATCAGACTACAATGCTCAAGGGGGAAACAGAAGAGATTGGGAAATTGATAGAGAAGACTATGATGCGCAAGTATGGAGTTGAAAATATTAATGAGCACTTCATTAGTTTCAATACGATCTGCGAAGCTACTCAAGTGTTGTCTTCCTCAGGAGCGCCAAGATGCATGTACCAGCTGGTGGAGGAAGATATGGATCTCATGTTAGTTGTAGGTGGATGGAACTCAAGCAACACCTCTCATCTTCAAGAGATTGCCGAGACTCGAGGAATCCCGTCGTATTGGATCGACAGCGAGCAACGAATAGGTCCAGGAAACTCAATATCATACAAGCTCACTCACGGGGAGCTGGCCAAGAAAGAAAACTGGCTACCTGATGGACATGTGACCATAGGAGTGACCTCCGGTGCCTCCACACCAGATAAGTCGAAGTTTAGTACTCAGTTAAGGATGTTTTATGATTCTCTGCAAAACCACAGTCCTCGCCCTCGTAATGTCTCGGCTACACATATCAGCTTGAGCCCCCAAATCCTCTACGCTTTTCATGAACACCGCCAGTTTCTTCTTCATCTCCTCCACCCCAACCTTCACAGCTTCCTCTTTAATGGCGAAATCTGCCTTCTGCAACATCCCCTCGATCTCAATCTCCAGTTTCTCAATCAGAATCCGAATGTTGTCCATGTCTTTCATGGCACGTACGTCCCCACTTGCATGGACTTATCACTTCCTTGTGGCCTTTCAGTTCATTCTCGTAGCTCTTCCACAGAGAATCGATCCACTTCCCCATGGAGCCGAGGGGTATGGAGGACGCTGCAGATAGCGCGCCGCGACCGGCGGAGCGGCCATGACTGTCGCCACCACCGAGCAGATCAACACGGCGGCGAAGGTGGCGACGAATATCATGCTCGACAGCTTCCTCCATGTGCGGATGGATTTGAGCTTCTTGTCGAGTTTGTTTTTTCTCAGCTGCAGTTTCTCGAGCATTATCGTTTGCTGTTTGTAAACGGAGTGGAAGATTTTGAAGAACTCCTCGGTGAAAGGGTGGCCGGAGGCCTTGAAATTCCTCAGTTCTTGCAGAGCACTTTTCCAAAGCAGTACAGAAATCGAGCGTCTGCAAACTGTTCTCGAAATACTCTTCGACGAGCTCGAACAGCTCCTGATTTTCCAGATGTCCTTTTTGCATTGCAATATAACTCTGACGACTTCTTGGTTCATTTCGAGCAAGCATTCTGTAACTTGCTTGAGAGAATCGAAGGAGAGGGCTTTGACTTCAACGCGACGGCCAAGCTGGTGATGACTTGGTTGGTGCGGGCATGGAGGTTCTCGTCGAAGGATCGGACGTCGACGTCGGCCCTGCAGGCTGCCGCATGGATTTTCTGTGAAAAGAGAAGAAAGAGCAGAAACCCAGTTTCAGAAAGACGACGATCTCTCGACTGGATCGAGAAGAAGAAGAAGGAGGAGGAGGAAGAAGAATGTGAAAAGGAAAGGCGGAGAGGGAAACTTGAAGTGAAAGAGAGAGGAAACAAAGATTGA

Protein sequence

MISRLPHRTDFVSPKIFAAGALRAGNFFPSGALPILRLLRSRSIRFRCEVVPEELGQRAVLLSLGADFVLNGSVERAVQIAYEARKQFPNGTIWITNEIIHNPTVNKRLEEMGVHNIPLEEGKKRFDVVNPGDVVILPAFGAAVDEMLVLNDKNVQIVDTTCPWVSKEKRHIAAVDAEIIPHFIRVEGFDPEIDLVKVGIANQTTMLKGETEEIGKLIEKTMMRKYGVENINEHFISFNTICEATQVLSSSGAPRCMYQLVEEDMDLMLVVGGWNSSNTSHLQEIAETRGIPSYWIDSEQRIGPGNSISYKLTHGELAKKENWLPDGHVTIGVTSGASTPDKSKFSTQLRMFYDSLQNHSPRPRNVSATHISLSPQILYAFHEHRQFLLHLLHPNLHSFLFNGEICLLQHPLDLNLQFLNQNPNVVHVFHGTYVPTCMDLSLPCGLSVHSRSSSTENRSTSPWSRGVWRTLQIARRDRRSGHDCRHHRADQHGGEGGDEYHARQLPPCADGFELLVEFVFSQLQFLEHYRLLFVNGVEDFEELLGERVAGGLEIPQFLQSTFPKQYRNRASANCSRNTLRRARTAPDFPDVLFALQYNSDDFLVHFEQAFCNLLERIEGEGFDFNATAKLVMTWLVRAWRFSSKDRTSTSALQAAAWIFCEKRRKSRNPVSERRRSLDWIEKKKKEEEEEECEKERRRGKLEVKERGNKD
Homology
BLAST of Sgr021206 vs. NCBI nr
Match: XP_023512039.1 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 445.3 bits (1144), Expect = 1.0e-120
Identity = 260/436 (59.63%), Postives = 277/436 (63.53%), Query Frame = 0

Query: 2   ISRLPHRTDFVSPKIFA-AGAL-------------------------------RAGNFFP 61
           I RLP R D VS K F+ AGAL                               R  N+  
Sbjct: 7   IPRLPRRVDSVSSKSFSGAGALRFRKPLLVRCTADSSSVNPDFDAKLFWKNFSRGENYNR 66

Query: 62  SGALPILRLLRSRSIRFRCEVVP--EELGQR----AVLLSLGADFVLNGSVERAVQIAYE 121
            G       L+  S  F  EVV    E G R     V + L   +     VERAVQIAYE
Sbjct: 67  KGFGYKEEALKLMSSEFTSEVVNTLNENGNRFTWGNVTVKLAGAYGFCWGVERAVQIAYE 126

Query: 122 ARKQFPNGTIWITNEIIHNPTVNKRLEEMGVHNIPLEEGKKRFDVVNPGDVVILPAFGAA 181
           ARKQFP+ TIWITNEIIHNPTVNKRLEEMGV+NIPLEEGKKRFDVVNPGDVVILPAFGAA
Sbjct: 127 ARKQFPSETIWITNEIIHNPTVNKRLEEMGVYNIPLEEGKKRFDVVNPGDVVILPAFGAA 186

Query: 182 VDEMLVLNDKNVQIVDTTCPWVSK-------------------EKRHIAAVDAE------ 241
           VDEMLVLNDK V+IVDTTCPWVSK                   + RH   V         
Sbjct: 187 VDEMLVLNDKKVKIVDTTCPWVSKVWNAVDKHKKGEYTSIIHGKYRHEETVATASFAGKY 246

Query: 242 -----------IIPHFIRVE---------------------GFDPEIDLVKVGIANQTTM 301
                      +  + + VE                     GFDP+ DL+KVGIANQTTM
Sbjct: 247 IIVKDIVEATYVCDYILGVESNGLISTKEAFLKKFKSAVSKGFDPDSDLMKVGIANQTTM 306

Query: 302 LKGETEEIGKLIEKTMMRKYGVENINEHFISFNTICEATQVLSSSGAPRCMYQLVEEDMD 343
           LKGETEEIGKLIEKTMMRKYG+ENIN+HFISFNTIC ATQV   +     MYQLVEEDMD
Sbjct: 307 LKGETEEIGKLIEKTMMRKYGIENINDHFISFNTICNATQVRQDA-----MYQLVEEDMD 366

BLAST of Sgr021206 vs. NCBI nr
Match: KAG7010948.1 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 441.4 bits (1134), Expect = 1.4e-119
Identity = 247/391 (63.17%), Postives = 265/391 (67.77%), Query Frame = 0

Query: 15  KIFAAGALRAGNFFPSGALPILRLLRSRSIRFRCEVVP--EELGQR----AVLLSLGADF 74
           K+F     R  N+   G       L+  S  F  EVV    E G R     V + L   +
Sbjct: 52  KLFWKNFSRGENYNRKGFGHKEETLKLMSSEFTSEVVNTLNENGNRFTWGNVTVKLAGAY 111

Query: 75  VLNGSVERAVQIAYEARKQFPNGTIWITNEIIHNPTVNKRLEEMGVHNIPLEEGKKRFDV 134
                VERAVQIAYEARKQFP+ TIWITNEIIHNPTVNKRLEEMGV+NIPLEEGKKRFDV
Sbjct: 112 GFCWGVERAVQIAYEARKQFPSETIWITNEIIHNPTVNKRLEEMGVYNIPLEEGKKRFDV 171

Query: 135 VNPGDVVILPAFGAAVDEMLVLNDKNVQIVDTTCPWVSK-------------------EK 194
           VNPGDVVILPAFGAAVDEMLVL+DK V+IVDTTCPWVSK                   + 
Sbjct: 172 VNPGDVVILPAFGAAVDEMLVLSDKKVKIVDTTCPWVSKVWNAVDKHKKGEYTSIIHGKY 231

Query: 195 RHIAAVDAE-----------------IIPHFIRVE---------------------GFDP 254
           RH   V                    +  + + VE                     GFDP
Sbjct: 232 RHEETVATASFAGKYIIVKDMVEATYVCDYILGVESNGLISTKEAFLKKFKSALSKGFDP 291

Query: 255 EIDLVKVGIANQTTMLKGETEEIGKLIEKTMMRKYGVENINEHFISFNTICEATQVLSSS 314
           + DL+KVGIANQTTMLKGETEEIGKLIEKTMMRKYG+ENIN+HFISFNTIC ATQV   +
Sbjct: 292 DSDLMKVGIANQTTMLKGETEEIGKLIEKTMMRKYGIENINDHFISFNTICNATQVRQDA 351

Query: 315 GAPRCMYQLVEEDMDLMLVVGGWNSSNTSHLQEIAETRGIPSYWIDSEQRIGPGNSISYK 343
                MYQLVEEDMDLMLVVGGWNSSNTSHLQEIAE RGIPSYWIDSEQRIGPGNSISYK
Sbjct: 352 -----MYQLVEEDMDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYK 411

BLAST of Sgr021206 vs. NCBI nr
Match: XP_022944389.1 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 441.4 bits (1134), Expect = 1.4e-119
Identity = 247/391 (63.17%), Postives = 265/391 (67.77%), Query Frame = 0

Query: 15  KIFAAGALRAGNFFPSGALPILRLLRSRSIRFRCEVVP--EELGQR----AVLLSLGADF 74
           K+F     R  N+   G       L+  S  F  EVV    E G R     V + L   +
Sbjct: 52  KLFWKNFSRGENYNRKGFGHKEETLKLMSSEFTSEVVNTLNENGNRFTWGNVTVKLAGAY 111

Query: 75  VLNGSVERAVQIAYEARKQFPNGTIWITNEIIHNPTVNKRLEEMGVHNIPLEEGKKRFDV 134
                VERAVQIAYEARKQFP+ TIWITNEIIHNPTVNKRLEEMGV+NIPLEEGKKRFDV
Sbjct: 112 GFCWGVERAVQIAYEARKQFPSETIWITNEIIHNPTVNKRLEEMGVYNIPLEEGKKRFDV 171

Query: 135 VNPGDVVILPAFGAAVDEMLVLNDKNVQIVDTTCPWVSK-------------------EK 194
           VNPGDVVILPAFGAAVDEMLVL+DK V+IVDTTCPWVSK                   + 
Sbjct: 172 VNPGDVVILPAFGAAVDEMLVLSDKKVKIVDTTCPWVSKVWNAVDKHKKGEYTSIIHGKY 231

Query: 195 RHIAAVDAE-----------------IIPHFIRVE---------------------GFDP 254
           RH   V                    +  + + VE                     GFDP
Sbjct: 232 RHEETVATASFAGKYIIVKDMVEATYVCDYILGVESNGLISTKEAFLKKFKSALSKGFDP 291

Query: 255 EIDLVKVGIANQTTMLKGETEEIGKLIEKTMMRKYGVENINEHFISFNTICEATQVLSSS 314
           + DL+KVGIANQTTMLKGETEEIGKLIEKTMMRKYG+ENIN+HFISFNTIC ATQV   +
Sbjct: 292 DSDLMKVGIANQTTMLKGETEEIGKLIEKTMMRKYGIENINDHFISFNTICNATQVRQDA 351

Query: 315 GAPRCMYQLVEEDMDLMLVVGGWNSSNTSHLQEIAETRGIPSYWIDSEQRIGPGNSISYK 343
                MYQLVEEDMDLMLVVGGWNSSNTSHLQEIAE RGIPSYWIDSEQRIGPGNSISYK
Sbjct: 352 -----MYQLVEEDMDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYK 411

BLAST of Sgr021206 vs. NCBI nr
Match: KAG6571137.1 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 441.4 bits (1134), Expect = 1.4e-119
Identity = 247/391 (63.17%), Postives = 265/391 (67.77%), Query Frame = 0

Query: 15  KIFAAGALRAGNFFPSGALPILRLLRSRSIRFRCEVVP--EELGQR----AVLLSLGADF 74
           K+F     R  N+   G       L+  S  F  EVV    E G R     V + L   +
Sbjct: 52  KLFWKNFSRGENYNRKGFGHKEETLKLMSSEFTSEVVNTLNENGNRFTWGNVTVKLAGAY 111

Query: 75  VLNGSVERAVQIAYEARKQFPNGTIWITNEIIHNPTVNKRLEEMGVHNIPLEEGKKRFDV 134
                VERAVQIAYEARKQFP+ TIWITNEIIHNPTVNKRLEEMGV+NIPLEEGKKRFDV
Sbjct: 112 GFCWGVERAVQIAYEARKQFPSETIWITNEIIHNPTVNKRLEEMGVYNIPLEEGKKRFDV 171

Query: 135 VNPGDVVILPAFGAAVDEMLVLNDKNVQIVDTTCPWVSK-------------------EK 194
           VNPGDVVILPAFGAAVDEMLVL+DK V+IVDTTCPWVSK                   + 
Sbjct: 172 VNPGDVVILPAFGAAVDEMLVLSDKKVKIVDTTCPWVSKVWNAVDKHKKGEYTSIIHGKY 231

Query: 195 RHIAAVDAE-----------------IIPHFIRVE---------------------GFDP 254
           RH   V                    +  + + VE                     GFDP
Sbjct: 232 RHEETVATASFAGKYIFVKDMAEATYVCDYILGVESNGLISTKEAFLKKFKSALSKGFDP 291

Query: 255 EIDLVKVGIANQTTMLKGETEEIGKLIEKTMMRKYGVENINEHFISFNTICEATQVLSSS 314
           + DL+KVGIANQTTMLKGETEEIGKLIEKTMMRKYG+ENIN+HFISFNTIC ATQV   +
Sbjct: 292 DSDLMKVGIANQTTMLKGETEEIGKLIEKTMMRKYGIENINDHFISFNTICNATQVRQDA 351

Query: 315 GAPRCMYQLVEEDMDLMLVVGGWNSSNTSHLQEIAETRGIPSYWIDSEQRIGPGNSISYK 343
                MYQLVEEDMDLMLVVGGWNSSNTSHLQEIAE RGIPSYWIDSEQRIGPGNSISYK
Sbjct: 352 -----MYQLVEEDMDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYK 411

BLAST of Sgr021206 vs. NCBI nr
Match: XP_023512040.1 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 440.7 bits (1132), Expect = 2.5e-119
Identity = 234/340 (68.82%), Postives = 248/340 (72.94%), Query Frame = 0

Query: 60  VLLSLGADFVLNGSVERAVQIAYEARKQFPNGTIWITNEIIHNPTVNKRLEEMGVHNIPL 119
           V + L   +     VERAVQIAYEARKQFP+ TIWITNEIIHNPTVNKRLEEMGV+NIPL
Sbjct: 25  VTVKLAGAYGFCWGVERAVQIAYEARKQFPSETIWITNEIIHNPTVNKRLEEMGVYNIPL 84

Query: 120 EEGKKRFDVVNPGDVVILPAFGAAVDEMLVLNDKNVQIVDTTCPWVSK------------ 179
           EEGKKRFDVVNPGDVVILPAFGAAVDEMLVLNDK V+IVDTTCPWVSK            
Sbjct: 85  EEGKKRFDVVNPGDVVILPAFGAAVDEMLVLNDKKVKIVDTTCPWVSKVWNAVDKHKKGE 144

Query: 180 -------EKRHIAAVDAE-----------------IIPHFIRVE---------------- 239
                  + RH   V                    +  + + VE                
Sbjct: 145 YTSIIHGKYRHEETVATASFAGKYIIVKDIVEATYVCDYILGVESNGLISTKEAFLKKFK 204

Query: 240 -----GFDPEIDLVKVGIANQTTMLKGETEEIGKLIEKTMMRKYGVENINEHFISFNTIC 299
                GFDP+ DL+KVGIANQTTMLKGETEEIGKLIEKTMMRKYG+ENIN+HFISFNTIC
Sbjct: 205 SAVSKGFDPDSDLMKVGIANQTTMLKGETEEIGKLIEKTMMRKYGIENINDHFISFNTIC 264

Query: 300 EATQVLSSSGAPRCMYQLVEEDMDLMLVVGGWNSSNTSHLQEIAETRGIPSYWIDSEQRI 343
            ATQV   +     MYQLVEEDMDLMLVVGGWNSSNTSHLQEIAE RGIPSYWIDSEQRI
Sbjct: 265 NATQVRQDA-----MYQLVEEDMDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRI 324

BLAST of Sgr021206 vs. ExPASy Swiss-Prot
Match: Q6AVG6 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISPH PE=2 SV=1)

HSP 1 Score: 387.9 bits (995), Expect = 2.5e-106
Identity = 203/326 (62.27%), Postives = 231/326 (70.86%), Query Frame = 0

Query: 74  VERAVQIAYEARKQFPNGTIWITNEIIHNPTVNKRLEEMGVHNIPLEEGKKRFDVVNPGD 133
           VERAVQIAYEARKQFP+  IW+TNEIIHNPTVNKRLE+MGV NIP++ G K FDVV  GD
Sbjct: 118 VERAVQIAYEARKQFPDDRIWLTNEIIHNPTVNKRLEDMGVQNIPVDAGIKDFDVVEQGD 177

Query: 134 VVILPAFGAAVDEMLVLNDKNVQIVDTTCPWVSK-----EK------------------- 193
           VV+LPAFGAAV+EM  LN+K VQIVDTTCPWVSK     EK                   
Sbjct: 178 VVVLPAFGAAVEEMYTLNEKKVQIVDTTCPWVSKVWNMVEKHKKGDYTSIIHGKYSHEET 237

Query: 194 -------------RHIA------------------AVDAEIIPHFIRV--EGFDPEIDLV 253
                        ++IA                  +   E +  F      GFDP++DLV
Sbjct: 238 VATASFAGTYIIVKNIAEASYVCDYILGGQLDGSSSTKEEFLEKFKNAVSPGFDPDVDLV 297

Query: 254 KVGIANQTTMLKGETEEIGKLIEKTMMRKYGVENINEHFISFNTICEATQVLSSSGAPRC 313
           KVGIANQTTMLKGETEEIGKL+EKTMMR++GVEN+N+HFI+FNTIC+ATQ    +     
Sbjct: 298 KVGIANQTTMLKGETEEIGKLVEKTMMRRFGVENVNDHFIAFNTICDATQERQDA----- 357

Query: 314 MYQLVEEDMDLMLVVGGWNSSNTSHLQEIAETRGIPSYWIDSEQRIGPGNSISYKLTHGE 343
           MYQLV+E +DL+LVVGGWNSSNTSHLQEI E  GIPSYWIDSEQRIGPGN ISYKL HGE
Sbjct: 358 MYQLVKEKVDLILVVGGWNSSNTSHLQEIGELSGIPSYWIDSEQRIGPGNKISYKLNHGE 417

BLAST of Sgr021206 vs. ExPASy Swiss-Prot
Match: Q94B35 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISPH PE=1 SV=1)

HSP 1 Score: 371.7 bits (953), Expect = 1.8e-101
Identity = 199/336 (59.23%), Postives = 231/336 (68.75%), Query Frame = 0

Query: 74  VERAVQIAYEARKQFPNGTIWITNEIIHNPTVNKRLEEMGVHNIPLEEGKKRFDVVNPGD 133
           VERAVQIAYEARKQFP   +WITNEIIHNPTVNKRLE+M V  IP+E+ KK+FDVV   D
Sbjct: 125 VERAVQIAYEARKQFPEERLWITNEIIHNPTVNKRLEDMDVKIIPVEDSKKQFDVVEKDD 184

Query: 134 VVILPAFGAAVDEMLVLNDKNVQIVDTTCPWVSK--------EKRHIAAV---------- 193
           VVILPAFGA VDEM VLNDK VQIVDTTCPWV+K        +K    +V          
Sbjct: 185 VVILPAFGAGVDEMYVLNDKKVQIVDTTCPWVTKVWNTVEKHKKGEYTSVIHGKYNHEET 244

Query: 194 -----------------DAEIIPHFIR----------------------VEGFDPEIDLV 253
                            +A  +  +I                        +GFDP+ DLV
Sbjct: 245 IATASFAGKYIIVKNMKEANYVCDYILGGQYDGSSSTKEEFMEKFKYAISKGFDPDNDLV 304

Query: 254 KVGIANQTTMLKGETEEIGKLIEKTMMRKYGVENINEHFISFNTICEATQVLSSSGAPRC 313
           KVGIANQTTMLKGETEEIG+L+E TMMRKYGVEN++ HFISFNTIC+ATQ    +     
Sbjct: 305 KVGIANQTTMLKGETEEIGRLLETTMMRKYGVENVSGHFISFNTICDATQERQDA----- 364

Query: 314 MYQLVEEDMDLMLVVGGWNSSNTSHLQEIAETRGIPSYWIDSEQRIGPGNSISYKLTHGE 353
           +Y+LVEE +DLMLVVGGWNSSNTSHLQEI+E RGIPSYWIDSE+RIGPGN I+YKL +GE
Sbjct: 365 IYELVEEKIDLMLVVGGWNSSNTSHLQEISEARGIPSYWIDSEKRIGPGNKIAYKLHYGE 424

BLAST of Sgr021206 vs. ExPASy Swiss-Prot
Match: B8HWD3 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Cyanothece sp. (strain PCC 7425 / ATCC 29141) OX=395961 GN=ispH PE=3 SV=1)

HSP 1 Score: 310.8 bits (795), Expect = 3.9e-83
Identity = 174/354 (49.15%), Postives = 222/354 (62.71%), Query Frame = 0

Query: 60  VLLSLGADFVLNGSVERAVQIAYEARKQFPNGTIWITNEIIHNPTVNKRLEEMGVHNIPL 119
           V + L   F     VERAV +AYE R+ FP   IWITNEIIHNP+VN+ L EM V  IP+
Sbjct: 55  VTIQLAEAFGFCWGVERAVALAYETRQHFPTERIWITNEIIHNPSVNQNLREMQVEFIPV 114

Query: 120 -EEGKKRFDVVNPGDVVILPAFGAAVDEMLVLNDKNVQIVDTTCPWVSK----------- 179
            E+G+K F VV   DVVILPAFGA+V EM +LNDK  +I+DTTCPWVSK           
Sbjct: 115 DEQGQKDFSVVAGEDVVILPAFGASVQEMQLLNDKGCKIMDTTCPWVSKVWNSVEKHKKG 174

Query: 180 --------EKRHIAAV----------------DAEIIPHFIRVEG--------------- 239
                   + +H   +                +AE + ++I   G               
Sbjct: 175 SYTSIIHGKYQHEETIATSSYAATYLIVLDLKEAEYVCNYILNGGDREEFLHKFRHAYSP 234

Query: 240 -FDPEIDLVKVGIANQTTMLKGETEEIGKLIEKTMMRKYGVENINEHFISFNTICEATQV 299
            FDP+ DLV+VGIANQTTMLKGETE+IGKL E+T+MRKYG + IN+HF+SFNTIC+ATQ 
Sbjct: 235 DFDPDRDLVRVGIANQTTMLKGETEQIGKLFERTLMRKYGPDQINQHFLSFNTICDATQE 294

Query: 300 LSSSGAPRCMYQLVEEDMDLMLVVGGWNSSNTSHLQEIAETRGIPSYWIDSEQRIGPGNS 359
              +     M++LVE  +DLM+V+GG+NSSNT+HLQEI+  R IPSY IDS  RIGPGN 
Sbjct: 295 RQDA-----MFKLVEYPLDLMVVIGGFNSSNTTHLQEISIEREIPSYHIDSADRIGPGNR 354

Query: 360 ISYKLTHGELAKKENWLPDGHVTIGVTSGASTPDKSKFSTQLRMFYDSLQNHSP 362
           + +K  H  L  KENWLP+G + +G+TSGASTPDK       ++F  +L++ SP
Sbjct: 355 VEHKPLHRPLEIKENWLPEGPIVVGITSGASTPDKVVSDVLEKIF--ALKSTSP 401

BLAST of Sgr021206 vs. ExPASy Swiss-Prot
Match: Q8DK29 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Thermosynechococcus elongatus (strain BP-1) OX=197221 GN=ispH PE=3 SV=1)

HSP 1 Score: 310.8 bits (795), Expect = 3.9e-83
Identity = 171/337 (50.74%), Postives = 205/337 (60.83%), Query Frame = 0

Query: 57  QRAVLLSLGADFVLNGSVERAVQIAYEARKQFPNGTIWITNEIIHNPTVNKRLEEMGVHN 116
           Q  V + L   F     VERAV +AYE R  FP   IWITNEIIHNP+VN+RL +M V  
Sbjct: 52  QGDVTIRLAEAFGFCWGVERAVALAYETRTHFPTERIWITNEIIHNPSVNERLRQMAVEF 111

Query: 117 IPLEEGKKRFDVVNPGDVVILPAFGAAVDEMLVLNDKNVQIVDTTCPWVSK--------- 176
           IP+  G K F  V PGDVVILPAFGA+V EM +LN++   IVDTTCPWVSK         
Sbjct: 112 IPVVNGVKDFSGVRPGDVVILPAFGASVQEMQLLNERGCTIVDTTCPWVSKVWHSVEKHK 171

Query: 177 ----------------------------------EKRHI------AAVDAEIIPHFIRV- 236
                                             E R++          A  +  F +  
Sbjct: 172 KVSFTSIIHGKYNHEETIATSSFAGTYLIVLNLEEARYVCDYILHGGDRAAFMAKFAKAC 231

Query: 237 -EGFDPEIDLVKVGIANQTTMLKGETEEIGKLIEKTMMRKYGVENINEHFISFNTICEAT 296
             GFDP+ DLV+VGIANQTTMLKGETE+IGKL E+TM++KYG + +NEHF+SFNTIC+AT
Sbjct: 232 SPGFDPDRDLVRVGIANQTTMLKGETEQIGKLFERTMIQKYGPDRLNEHFMSFNTICDAT 291

Query: 297 QVLSSSGAPRCMYQLVEEDMDLMLVVGGWNSSNTSHLQEIAETRGIPSYWIDSEQRIGPG 343
           Q    +     M  LV+E +DLM+V+GG+NSSNT+HLQEIA   GIPSY IDS  RIGPG
Sbjct: 292 QERQDA-----MLSLVKEPLDLMVVIGGYNSSNTTHLQEIAIEHGIPSYHIDSADRIGPG 351

BLAST of Sgr021206 vs. ExPASy Swiss-Prot
Match: B0C4N8 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Acaryochloris marina (strain MBIC 11017) OX=329726 GN=ispH PE=3 SV=1)

HSP 1 Score: 306.2 bits (783), Expect = 9.5e-82
Identity = 168/348 (48.28%), Postives = 213/348 (61.21%), Query Frame = 0

Query: 55  LGQRAVLLSLGADFVLNGSVERAVQIAYEARKQFPNGTIWITNEIIHNPTVNKRLEEMGV 114
           L Q  V + L   F     VERA+ +AYE R+QFP   IWITNEIIHNP+VNK L +M V
Sbjct: 50  LQQGDVTIHLAEAFGFCWGVERAIALAYETRRQFPQERIWITNEIIHNPSVNKNLRDMKV 109

Query: 115 HNIPLE-EGKKRFDVVNPGDVVILPAFGAAVDEMLVLNDKNVQIVDTTCPWVSK------ 174
             IP++ +G+K F VV   DVVILPAFGA+V EM +L+DK   I+DTTCPWVSK      
Sbjct: 110 EFIPVQPDGQKDFTVVGQNDVVILPAFGASVQEMQLLHDKGCTIMDTTCPWVSKVWTSVE 169

Query: 175 -------------EKRHIAAV----------------DAEIIPHFI-------------- 234
                        + +H   +                +AE +  +I              
Sbjct: 170 KHKKGSYTSIIHGKYKHEETIATSSFAGTYLVLLNLEEAEYVADYILNPGDTPSEREQRR 229

Query: 235 ----------RVEGFDPEIDLVKVGIANQTTMLKGETEEIGKLIEKTMMRKYGVENINEH 294
                        GFDPE DL +VGIANQTTMLKGETE+IGKL E+T+MRKYG   +NEH
Sbjct: 230 ADFMAKFANAHSPGFDPETDLERVGIANQTTMLKGETEQIGKLFERTVMRKYGPAQVNEH 289

Query: 295 FISFNTICEATQVLSSSGAPRCMYQLVEEDMDLMLVVGGWNSSNTSHLQEIAETRGIPSY 343
           F+SFNTIC+ATQ    +     ++QLV+E +DLM+V+GG+NSSNT+HLQEI+  + IPSY
Sbjct: 290 FLSFNTICDATQERQDA-----IFQLVDEPLDLMVVIGGFNSSNTTHLQEISIDKNIPSY 349

BLAST of Sgr021206 vs. ExPASy TrEMBL
Match: A0A6J1FWL7 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111448851 PE=3 SV=1)

HSP 1 Score: 441.4 bits (1134), Expect = 7.0e-120
Identity = 247/391 (63.17%), Postives = 265/391 (67.77%), Query Frame = 0

Query: 15  KIFAAGALRAGNFFPSGALPILRLLRSRSIRFRCEVVP--EELGQR----AVLLSLGADF 74
           K+F     R  N+   G       L+  S  F  EVV    E G R     V + L   +
Sbjct: 52  KLFWKNFSRGENYNRKGFGHKEETLKLMSSEFTSEVVNTLNENGNRFTWGNVTVKLAGAY 111

Query: 75  VLNGSVERAVQIAYEARKQFPNGTIWITNEIIHNPTVNKRLEEMGVHNIPLEEGKKRFDV 134
                VERAVQIAYEARKQFP+ TIWITNEIIHNPTVNKRLEEMGV+NIPLEEGKKRFDV
Sbjct: 112 GFCWGVERAVQIAYEARKQFPSETIWITNEIIHNPTVNKRLEEMGVYNIPLEEGKKRFDV 171

Query: 135 VNPGDVVILPAFGAAVDEMLVLNDKNVQIVDTTCPWVSK-------------------EK 194
           VNPGDVVILPAFGAAVDEMLVL+DK V+IVDTTCPWVSK                   + 
Sbjct: 172 VNPGDVVILPAFGAAVDEMLVLSDKKVKIVDTTCPWVSKVWNAVDKHKKGEYTSIIHGKY 231

Query: 195 RHIAAVDAE-----------------IIPHFIRVE---------------------GFDP 254
           RH   V                    +  + + VE                     GFDP
Sbjct: 232 RHEETVATASFAGKYIIVKDMVEATYVCDYILGVESNGLISTKEAFLKKFKSALSKGFDP 291

Query: 255 EIDLVKVGIANQTTMLKGETEEIGKLIEKTMMRKYGVENINEHFISFNTICEATQVLSSS 314
           + DL+KVGIANQTTMLKGETEEIGKLIEKTMMRKYG+ENIN+HFISFNTIC ATQV   +
Sbjct: 292 DSDLMKVGIANQTTMLKGETEEIGKLIEKTMMRKYGIENINDHFISFNTICNATQVRQDA 351

Query: 315 GAPRCMYQLVEEDMDLMLVVGGWNSSNTSHLQEIAETRGIPSYWIDSEQRIGPGNSISYK 343
                MYQLVEEDMDLMLVVGGWNSSNTSHLQEIAE RGIPSYWIDSEQRIGPGNSISYK
Sbjct: 352 -----MYQLVEEDMDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYK 411

BLAST of Sgr021206 vs. ExPASy TrEMBL
Match: A0A6J1FYM6 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111448851 PE=3 SV=1)

HSP 1 Score: 438.7 bits (1127), Expect = 4.5e-119
Identity = 233/340 (68.53%), Postives = 248/340 (72.94%), Query Frame = 0

Query: 60  VLLSLGADFVLNGSVERAVQIAYEARKQFPNGTIWITNEIIHNPTVNKRLEEMGVHNIPL 119
           V + L   +     VERAVQIAYEARKQFP+ TIWITNEIIHNPTVNKRLEEMGV+NIPL
Sbjct: 25  VTVKLAGAYGFCWGVERAVQIAYEARKQFPSETIWITNEIIHNPTVNKRLEEMGVYNIPL 84

Query: 120 EEGKKRFDVVNPGDVVILPAFGAAVDEMLVLNDKNVQIVDTTCPWVSK------------ 179
           EEGKKRFDVVNPGDVVILPAFGAAVDEMLVL+DK V+IVDTTCPWVSK            
Sbjct: 85  EEGKKRFDVVNPGDVVILPAFGAAVDEMLVLSDKKVKIVDTTCPWVSKVWNAVDKHKKGE 144

Query: 180 -------EKRHIAAVDAE-----------------IIPHFIRVE---------------- 239
                  + RH   V                    +  + + VE                
Sbjct: 145 YTSIIHGKYRHEETVATASFAGKYIIVKDMVEATYVCDYILGVESNGLISTKEAFLKKFK 204

Query: 240 -----GFDPEIDLVKVGIANQTTMLKGETEEIGKLIEKTMMRKYGVENINEHFISFNTIC 299
                GFDP+ DL+KVGIANQTTMLKGETEEIGKLIEKTMMRKYG+ENIN+HFISFNTIC
Sbjct: 205 SALSKGFDPDSDLMKVGIANQTTMLKGETEEIGKLIEKTMMRKYGIENINDHFISFNTIC 264

Query: 300 EATQVLSSSGAPRCMYQLVEEDMDLMLVVGGWNSSNTSHLQEIAETRGIPSYWIDSEQRI 343
            ATQV   +     MYQLVEEDMDLMLVVGGWNSSNTSHLQEIAE RGIPSYWIDSEQRI
Sbjct: 265 NATQVRQDA-----MYQLVEEDMDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRI 324

BLAST of Sgr021206 vs. ExPASy TrEMBL
Match: A0A5D3CQA5 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold299G001350 PE=3 SV=1)

HSP 1 Score: 431.8 bits (1109), Expect = 5.6e-117
Identity = 245/415 (59.04%), Postives = 270/415 (65.06%), Query Frame = 0

Query: 4   RLPHRTDFVSPKIFA-AGALRAGNFF--------PSGALPI-----LRLLRSRSIRFRCE 63
           R PHR DF  PK F+  GALR              S   P+      +L      R    
Sbjct: 9   RFPHRIDFSLPKTFSGVGALRCRQPLLLRCTADSSSSPKPVDPDFNAKLFLKNFSRGEIV 68

Query: 64  VVPEELGQR----AVLLSLGADFVLNGSVERAVQIAYEARKQFPNGTIWITNEIIHNPTV 123
              +E G +     V + L   +     VERAVQ AYEARKQ+P+ TIWITNEIIHNPTV
Sbjct: 69  TTLKENGFQFTWGNVTVKLAGAYGFCWGVERAVQFAYEARKQYPSETIWITNEIIHNPTV 128

Query: 124 NKRLEEMGVHNIPLEEG-KKRFDVVNPGDVVILPAFGAAVDEMLVLNDKNVQIVDTTCPW 183
           NKRLEEMGVHNIPLEEG KKRFD VNPGDVVILPAFGAAVDE+LVLNDK V+IVDTTCPW
Sbjct: 129 NKRLEEMGVHNIPLEEGNKKRFDAVNPGDVVILPAFGAAVDEILVLNDKKVKIVDTTCPW 188

Query: 184 VSK--------------------------------EKRHIAAVD----AEIIPHFIRVE- 243
           VSK                                  ++I   D    A +  + + VE 
Sbjct: 189 VSKVWNAVGKHKKGEYTSIIHGKYSHEETIATASFAGKYIIVKDMAEAAYVSDYILGVEH 248

Query: 244 --------------------GFDPEIDLVKVGIANQTTMLKGETEEIGKLIEKTMMRKYG 303
                               GFDP+I LVK+GIANQTTMLKGETEEIGKL+EKTMMRKYG
Sbjct: 249 DGSISTKEEFLKKFKSAVSKGFDPDIHLVKIGIANQTTMLKGETEEIGKLMEKTMMRKYG 308

Query: 304 VENINEHFISFNTICEATQVLSSSGAPRCMYQLVEEDMDLMLVVGGWNSSNTSHLQEIAE 343
           VENINEHFISFNTIC ATQV   +     MY+LV+ED+DLMLVVGGWNSSNTSHLQEIAE
Sbjct: 309 VENINEHFISFNTICNATQVRQDA-----MYELVKEDVDLMLVVGGWNSSNTSHLQEIAE 368

BLAST of Sgr021206 vs. ExPASy TrEMBL
Match: A0A6J1D5C2 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111017170 PE=3 SV=1)

HSP 1 Score: 430.3 bits (1105), Expect = 1.6e-116
Identity = 226/326 (69.33%), Postives = 242/326 (74.23%), Query Frame = 0

Query: 74  VERAVQIAYEARKQFPNGTIWITNEIIHNPTVNKRLEEMGVHNIPLEEGKKRFDVVNPGD 133
           VERAVQIA+EARKQFP+ TIWITNEIIHNPTVNKRL EMGVH IPLEE KK+FDVVNPGD
Sbjct: 123 VERAVQIAFEARKQFPSRTIWITNEIIHNPTVNKRLGEMGVHYIPLEERKKQFDVVNPGD 182

Query: 134 VVILPAFGAAVDEMLVLNDKNVQIVDTTCPWVSK--------EKRHIAAV---------- 193
           VVILPAFGAAVDEMLVL DKNVQIVDTTCPWVSK        +KR   ++          
Sbjct: 183 VVILPAFGAAVDEMLVLKDKNVQIVDTTCPWVSKVWNVVDKHKKREYTSIIHGKYRHEET 242

Query: 194 -----------------DAEIIPHFIR----------------------VEGFDPEIDLV 253
                            +A  +  FI                         GFDP+ DL+
Sbjct: 243 IATASFAGKYIIVKNMAEATYVSDFILGVERNGSILTKEEFLKKFESAVSRGFDPDYDLI 302

Query: 254 KVGIANQTTMLKGETEEIGKLIEKTMMRKYGVENINEHFISFNTICEATQVLSSSGAPRC 313
           KVGIANQTTMLKGETEEIGKLIE+TMMRKYGVEN+NEHF+SFNTIC+ATQV         
Sbjct: 303 KVGIANQTTMLKGETEEIGKLIERTMMRKYGVENVNEHFVSFNTICDATQVKRQD----A 362

Query: 314 MYQLVEEDMDLMLVVGGWNSSNTSHLQEIAETRGIPSYWIDSEQRIGPGNSISYKLTHGE 343
           MYQLVEEDMDLMLVVGGWNSSNTSHLQEIAE RGIPSYWIDSEQRIGPGNSISYKL +GE
Sbjct: 363 MYQLVEEDMDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLIYGE 422

BLAST of Sgr021206 vs. ExPASy TrEMBL
Match: A0A6J1JG99 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111484238 PE=3 SV=1)

HSP 1 Score: 428.7 bits (1101), Expect = 4.7e-116
Identity = 238/391 (60.87%), Postives = 259/391 (66.24%), Query Frame = 0

Query: 15  KIFAAGALRAGNFFPSGALPILRLLRSRSIRFRCEVVP--EELGQR----AVLLSLGADF 74
           K+F     R  N+   G       L+  S  F  EVV    E G R     V + L   +
Sbjct: 52  KLFWKNFSRGENYNRKGFEHKEETLKLMSSEFTSEVVNTLNENGNRFTWGNVTVKLAGAY 111

Query: 75  VLNGSVERAVQIAYEARKQFPNGTIWITNEIIHNPTVNKRLEEMGVHNIPLEEGKKRFDV 134
                VERAVQIAYEARKQFP+ TIWITNEIIHNPTVNKRLEEMGV+NIPLEEGKKRFDV
Sbjct: 112 GFCWGVERAVQIAYEARKQFPSETIWITNEIIHNPTVNKRLEEMGVYNIPLEEGKKRFDV 171

Query: 135 VNPGDVVILPAFGAAVDEMLVLNDKNVQIVDTTCPWVSK--------------------- 194
           VNPGDVVILPAFGAAVDEMLVL+DK V IVDTTCPWVSK                     
Sbjct: 172 VNPGDVVILPAFGAAVDEMLVLSDKKVTIVDTTCPWVSKVWNAVDKHKKGEYTSIIHGKY 231

Query: 195 ----------------------------------EKRHIAAVDAEIIPHFIRV--EGFDP 254
                                             E   + +   + +  F     +GFDP
Sbjct: 232 HHEETVATASFAGKYIIVKDMVEATYVCDYILGVESNGLISTKEDFLKKFKSAVSKGFDP 291

Query: 255 EIDLVKVGIANQTTMLKGETEEIGKLIEKTMMRKYGVENINEHFISFNTICEATQVLSSS 314
           + DL+KVGIANQTTMLK ET+EIGKLIEKTMMRKYG+ENI+EHFISFNTIC ATQV   +
Sbjct: 292 DSDLMKVGIANQTTMLKEETKEIGKLIEKTMMRKYGIENISEHFISFNTICNATQVRQDA 351

Query: 315 GAPRCMYQLVEEDMDLMLVVGGWNSSNTSHLQEIAETRGIPSYWIDSEQRIGPGNSISYK 343
                MYQLVEED DLMLVVGGWNSSNTSHLQEIAE RGIPSYWIDSEQRIGPGNSISYK
Sbjct: 352 -----MYQLVEEDTDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYK 411

BLAST of Sgr021206 vs. TAIR 10
Match: AT4G34350.1 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase )

HSP 1 Score: 371.7 bits (953), Expect = 1.3e-102
Identity = 199/336 (59.23%), Postives = 231/336 (68.75%), Query Frame = 0

Query: 74  VERAVQIAYEARKQFPNGTIWITNEIIHNPTVNKRLEEMGVHNIPLEEGKKRFDVVNPGD 133
           VERAVQIAYEARKQFP   +WITNEIIHNPTVNKRLE+M V  IP+E+ KK+FDVV   D
Sbjct: 125 VERAVQIAYEARKQFPEERLWITNEIIHNPTVNKRLEDMDVKIIPVEDSKKQFDVVEKDD 184

Query: 134 VVILPAFGAAVDEMLVLNDKNVQIVDTTCPWVSK--------EKRHIAAV---------- 193
           VVILPAFGA VDEM VLNDK VQIVDTTCPWV+K        +K    +V          
Sbjct: 185 VVILPAFGAGVDEMYVLNDKKVQIVDTTCPWVTKVWNTVEKHKKGEYTSVIHGKYNHEET 244

Query: 194 -----------------DAEIIPHFIR----------------------VEGFDPEIDLV 253
                            +A  +  +I                        +GFDP+ DLV
Sbjct: 245 IATASFAGKYIIVKNMKEANYVCDYILGGQYDGSSSTKEEFMEKFKYAISKGFDPDNDLV 304

Query: 254 KVGIANQTTMLKGETEEIGKLIEKTMMRKYGVENINEHFISFNTICEATQVLSSSGAPRC 313
           KVGIANQTTMLKGETEEIG+L+E TMMRKYGVEN++ HFISFNTIC+ATQ    +     
Sbjct: 305 KVGIANQTTMLKGETEEIGRLLETTMMRKYGVENVSGHFISFNTICDATQERQDA----- 364

Query: 314 MYQLVEEDMDLMLVVGGWNSSNTSHLQEIAETRGIPSYWIDSEQRIGPGNSISYKLTHGE 353
           +Y+LVEE +DLMLVVGGWNSSNTSHLQEI+E RGIPSYWIDSE+RIGPGN I+YKL +GE
Sbjct: 365 IYELVEEKIDLMLVVGGWNSSNTSHLQEISEARGIPSYWIDSEKRIGPGNKIAYKLHYGE 424

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023512039.11.0e-12059.634-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like isoform X... [more]
KAG7010948.11.4e-11963.174-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic, partial [Cucu... [more]
XP_022944389.11.4e-11963.174-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like isoform X... [more]
KAG6571137.11.4e-11963.174-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic, partial [Cucu... [more]
XP_023512040.12.5e-11968.824-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like isoform X... [more]
Match NameE-valueIdentityDescription
Q6AVG62.5e-10662.274-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic OS=Oryza sativ... [more]
Q94B351.8e-10159.234-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic OS=Arabidopsis... [more]
B8HWD33.9e-8349.154-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Cyanothece sp. (strain PCC... [more]
Q8DK293.9e-8350.744-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Thermosynechococcus elonga... [more]
B0C4N89.5e-8248.284-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Acaryochloris marina (stra... [more]
Match NameE-valueIdentityDescription
A0A6J1FWL77.0e-12063.174-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like isoform X... [more]
A0A6J1FYM64.5e-11968.534-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like isoform X... [more]
A0A5D3CQA55.6e-11759.044-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Cucumis melo var. makuwa O... [more]
A0A6J1D5C21.6e-11669.334-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like OS=Momord... [more]
A0A6J1JG994.7e-11660.874-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like isoform X... [more]
Match NameE-valueIdentityDescription
AT4G34350.11.3e-10259.234-hydroxy-3-methylbut-2-enyl diphosphate reductase [more]
InterPro
Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 682..702
NoneNo IPR availableGENE3D3.40.1010.20coord: 224..356
e-value: 6.1E-29
score: 101.9
NoneNo IPR availableGENE3D3.40.50.11270coord: 72..181
e-value: 6.2E-24
score: 86.0
NoneNo IPR availablePANTHERPTHR316194-HYDROXY-3-METHYLBUT-2-ENYL DIPHOSPHATE REDUCTASE, CHLOROPLASTICcoord: 186..353
coord: 68..168
IPR0034514-hydroxy-3-methylbut-2-enyl diphosphate reductasePFAMPF02401LYTBcoord: 193..342
e-value: 6.4E-28
score: 97.8
coord: 74..176
e-value: 1.4E-21
score: 77.0

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sgr021206.1Sgr021206.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0050992 dimethylallyl diphosphate biosynthetic process
biological_process GO:0019288 isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway
molecular_function GO:0051745 4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase activity
molecular_function GO:0051536 iron-sulfur cluster binding
molecular_function GO:0046872 metal ion binding