Sgr020616 (gene) Monk fruit (Qingpiguo) v1

BLAST of Sgr020616 vs. NCBI nr
Match: XP_038904957.1 (acyl-coenzyme A oxidase, peroxisomal [Benincasa hispida])

HSP 1 Score: 1064.7 bits (2752), Expect = 4.9e-307
Identity = 541/686 (78.86%), Postives = 578/686 (84.26%), Query Frame = 0

Query: 2   SSGEPNQMAEDESQAVSRRIQRLSFHLTPIPLPCGGSHQLEMLTCS-AAKAKLEVDIASL 61
           S+ EPN  A++ESQA SRRI+RLS HLTPIPLP  GS+++E+ TC+  +KAK+EVD+ SL
Sbjct: 3   SATEPNWTAKEESQAASRRIERLSLHLTPIPLPGDGSYRIEIATCAGTSKAKIEVDMGSL 62

Query: 62  SLYMRGKHREIQERVFEYFNSRPELQTPVEISMSDHRELCMKQLIGLVREAGIRPYRYVN 121
           S+YMRGKHREIQERVF+YFNSRPELQTPVEISM+DHRELCMKQLIGLVREAGIRP+R+VN
Sbjct: 63  SVYMRGKHREIQERVFDYFNSRPELQTPVEISMNDHRELCMKQLIGLVREAGIRPFRFVN 122

Query: 122 EDPAKYFAIMEAVGSIDVSFAIKMGVQFSLWGGSVLNLGTKKHRDKFFDGIDNVDYPGCF 181
           EDPA+YFAIMEAVGS+DVS AIKMGVQFSLWGGSVLNLGTKKHRDKFFDGIDNVDYPGCF
Sbjct: 123 EDPAQYFAIMEAVGSVDVSLAIKMGVQFSLWGGSVLNLGTKKHRDKFFDGIDNVDYPGCF 182

Query: 182 AMTELHHGSNVQGLQTTATFDPITDEFIINTPNDGAIKWWIGNAAVHGKFATVFAKLVLP 241
           AMTELHHGSNVQGLQTTATFDPITDEFIINTPNDGAIKWWIGNAAVHGKFATVFAKLVLP
Sbjct: 183 AMTELHHGSNVQGLQTTATFDPITDEFIINTPNDGAIKWWIGNAAVHGKFATVFAKLVLP 242

Query: 242 THDSKKIADMGVHAFIVPIRDLKSHKTLPGIEIHDCGHKIGLNGVDNGALRFRSVRIPRD 301
           THD KK ADMGVHAFIVPIRDLK+HKTLPGIEIHDCGHK+GLNGVDNGALRFRSVRIPRD
Sbjct: 243 THDYKKTADMGVHAFIVPIRDLKTHKTLPGIEIHDCGHKVGLNGVDNGALRFRSVRIPRD 302

Query: 302 NLLDRFGEVSRDGKYKSSLPSISKRFAATLGELVGGRVGLAYSSASVLKVASTIAIRYSL 361
           NLLDRFGEVSRDGKYKSSLPSI+KRFAATLGELVGGRVGLAYSSASVLK+ASTIAIRYSL
Sbjct: 303 NLLDRFGEVSRDGKYKSSLPSINKRFAATLGELVGGRVGLAYSSASVLKIASTIAIRYSL 362

Query: 362 LRQQFGPLKQPEVSILDYQSQQHKLMPMLASTYAFHFATLQLVEKYAQMKKTHDEQL-AE 421
           LRQQFGP KQPEVSILDYQSQQHKLMPMLASTYAF FATLQLVEKYAQMKKTHDE+L  +
Sbjct: 363 LRQQFGPPKQPEVSILDYQSQQHKLMPMLASTYAFQFATLQLVEKYAQMKKTHDEELVGD 422

Query: 422 IH---------------------------------------------------------- 481
           +H                                                          
Sbjct: 423 VHALSAGLKAYITSYTAKSLSTCREACGGHGYAAVNRFGTLRNDHDIFQTFEGDNTVLLQ 482

Query: 482 -----LLKQYKEKFEGGTLTVTWNYLRESMSTYLSQPNPVTARWE--------------- 541
                LLKQYKEKF+GGTL VTWNYLRESM+TYLSQPNPVTARWE               
Sbjct: 483 QVAAYLLKQYKEKFQGGTLAVTWNYLRESMNTYLSQPNPVTARWESVDHLRDPKFQLDAF 542

Query: 542 -IPNVSITSSAAVRLRKHTKTLGSFGAWNRCLNHLLTLAESHIESVILAKFIEAVQSCPN 601
                 +  S AVRLRKHTKTLG FGAWNRCLNHLLTLAESHIESVILA+FIEA+Q CPN
Sbjct: 543 QYRTSRLLQSVAVRLRKHTKTLGGFGAWNRCLNHLLTLAESHIESVILAQFIEAIQRCPN 602

Query: 602 ASTQATLKLVCDLYALDRIWNDIGTYRNVDYVAPNKAKAIHKLTEYLCFQVRNIAKELVD 607
           A+TQATLKLVCDLYALDRIWNDIGTYRNVDYVAPNKAKAIHKLTEYLCFQVRNIA+ELVD
Sbjct: 603 ANTQATLKLVCDLYALDRIWNDIGTYRNVDYVAPNKAKAIHKLTEYLCFQVRNIAQELVD 662

BLAST of Sgr020616 vs. NCBI nr
Match: XP_022984291.1 (acyl-coenzyme A oxidase, peroxisomal [Cucurbita maxima] >XP_022984292.1 acyl-coenzyme A oxidase, peroxisomal [Cucurbita maxima] >O64894.1 RecName: Full=Acyl-coenzyme A oxidase, peroxisomal; Short=AOX; AltName: Full=Long-chain acyl-CoA oxidase; Flags: Precursor [Cucurbita maxima] >pir|T07901| acyl CoA oxidase homolog - cucurbit >AAC15870.1 acyl CoA oxidase homolog [Cucurbita hybrid cultivar])

HSP 1 Score: 1058.1 bits (2735), Expect = 4.6e-305
Identity = 540/687 (78.60%), Postives = 578/687 (84.13%), Query Frame = 0

Query: 2   SSGEPNQMAEDESQAVSRRIQRLSFHLTPIPLPCGGSHQLEMLTCSA--AKAKLEVDIAS 61
           S GEPN+ AEDESQA +RRI+RLS HLTPIPL    S  +EM TC+A  AKAK+EVD+ S
Sbjct: 3   SPGEPNRTAEDESQAAARRIERLSLHLTPIPL--DDSQGVEMETCAAGKAKAKIEVDMGS 62

Query: 62  LSLYMRGKHREIQERVFEYFNSRPELQTPVEISMSDHRELCMKQLIGLVREAGIRPYRYV 121
           LSLYMRGKHREIQERVFEYFNSRPELQTPV ISM+DHRELCMKQL+GLVREAGIRP+R+V
Sbjct: 63  LSLYMRGKHREIQERVFEYFNSRPELQTPVGISMADHRELCMKQLVGLVREAGIRPFRFV 122

Query: 122 NEDPAKYFAIMEAVGSIDVSFAIKMGVQFSLWGGSVLNLGTKKHRDKFFDGIDNVDYPGC 181
           NEDPAKYFAIMEAVGS+DVS AIKMGVQFSLWGGSV+NLGTKKHRD+FFDGIDNVDYPGC
Sbjct: 123 NEDPAKYFAIMEAVGSVDVSLAIKMGVQFSLWGGSVINLGTKKHRDRFFDGIDNVDYPGC 182

Query: 182 FAMTELHHGSNVQGLQTTATFDPITDEFIINTPNDGAIKWWIGNAAVHGKFATVFAKLVL 241
           FAMTELHHGSNVQGLQTTATFDPITDEFIINTPNDGAIKWWIGNAAVHGKFATVFAKLVL
Sbjct: 183 FAMTELHHGSNVQGLQTTATFDPITDEFIINTPNDGAIKWWIGNAAVHGKFATVFAKLVL 242

Query: 242 PTHDSKKIADMGVHAFIVPIRDLKSHKTLPGIEIHDCGHKIGLNGVDNGALRFRSVRIPR 301
           PTHDS+K ADMGVHAFIVPIRDLKSHKTLPGIEIHDCGHK+GLNGVDNGALRFRSVRIPR
Sbjct: 243 PTHDSRKTADMGVHAFIVPIRDLKSHKTLPGIEIHDCGHKVGLNGVDNGALRFRSVRIPR 302

Query: 302 DNLLDRFGEVSRDGKYKSSLPSISKRFAATLGELVGGRVGLAYSSASVLKVASTIAIRYS 361
           DNLL+RFGEVSRDGKYKSSLPSI+KRFAATLGELVGGRVGLAYSSASVLK+ASTIAIRYS
Sbjct: 303 DNLLNRFGEVSRDGKYKSSLPSINKRFAATLGELVGGRVGLAYSSASVLKIASTIAIRYS 362

Query: 362 LLRQQFGPLKQPEVSILDYQSQQHKLMPMLASTYAFHFATLQLVEKYAQMKKTHDEQL-A 421
           LLRQQFGP KQPEVSILDYQSQQHKLMPMLASTYAFHF+T+QLVEKYAQMKKTHDE+L  
Sbjct: 363 LLRQQFGPPKQPEVSILDYQSQQHKLMPMLASTYAFHFSTMQLVEKYAQMKKTHDEELVG 422

Query: 422 EIH--------------------------------------------------------- 481
           ++H                                                         
Sbjct: 423 DVHALSAGLKAYVTSYTAKSLSTCREACGGHGYAVVNRFGTLRNDHDIFQTFEGDNTVLL 482

Query: 482 ------LLKQYKEKFEGGTLTVTWNYLRESMSTYLSQPNPVTARWEIPN----------- 541
                 LLKQY+EKF+GGTL VTWNYLRESM+TYLSQPNPVTARWE  +           
Sbjct: 483 QQVAAYLLKQYQEKFQGGTLAVTWNYLRESMNTYLSQPNPVTARWESADHLRDPKFQLDA 542

Query: 542 -----VSITSSAAVRLRKHTKTLGSFGAWNRCLNHLLTLAESHIESVILAKFIEAVQSCP 601
                  +  S AVRLRKHTK LGSFGAWNRCLNHLLTLAESHIESVILA+FIE+VQ CP
Sbjct: 543 FQYRTSRLLQSVAVRLRKHTKNLGSFGAWNRCLNHLLTLAESHIESVILAQFIESVQRCP 602

Query: 602 NASTQATLKLVCDLYALDRIWNDIGTYRNVDYVAPNKAKAIHKLTEYLCFQVRNIAKELV 607
           NA+TQATLKLVCDLYALDRIWNDIGTYRNVDYVAPNKAKAIHKLTEYLCFQVRNIA+ELV
Sbjct: 603 NANTQATLKLVCDLYALDRIWNDIGTYRNVDYVAPNKAKAIHKLTEYLCFQVRNIAQELV 662

BLAST of Sgr020616 vs. NCBI nr
Match: KAG6577284.1 (Acyl-coenzyme A oxidase, peroxisomal, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1057.4 bits (2733), Expect = 7.8e-305
Identity = 540/687 (78.60%), Postives = 575/687 (83.70%), Query Frame = 0

Query: 2   SSGEPNQMAEDESQAVSRRIQRLSFHLTPIPLPCGGSHQLEMLTCSA--AKAKLEVDIAS 61
           S GEPN+ AEDESQA +RRI+RLS HLTPIP     S  +EM TC A  AKAK+EVD+ S
Sbjct: 3   SPGEPNRTAEDESQAAARRIERLSLHLTPIPF--DDSQGVEMETCGAGKAKAKIEVDMGS 62

Query: 62  LSLYMRGKHREIQERVFEYFNSRPELQTPVEISMSDHRELCMKQLIGLVREAGIRPYRYV 121
           LSLYMRGKHRE+QERVFEYFNSRPELQTPV ISM+DHRELCMKQL+GLVREAGIRP+R+V
Sbjct: 63  LSLYMRGKHREVQERVFEYFNSRPELQTPVGISMADHRELCMKQLVGLVREAGIRPFRFV 122

Query: 122 NEDPAKYFAIMEAVGSIDVSFAIKMGVQFSLWGGSVLNLGTKKHRDKFFDGIDNVDYPGC 181
           NEDPAKYFAIMEAVGS+DVS AIKMGVQFSLWGGSV+NLGTKKHRD+FFDGIDNVDYPGC
Sbjct: 123 NEDPAKYFAIMEAVGSVDVSLAIKMGVQFSLWGGSVINLGTKKHRDRFFDGIDNVDYPGC 182

Query: 182 FAMTELHHGSNVQGLQTTATFDPITDEFIINTPNDGAIKWWIGNAAVHGKFATVFAKLVL 241
           FAMTELHHGSNVQGLQTTATFDPITDEFIINTPNDGAIKWWIGNAAVHGKFATVFAKLVL
Sbjct: 183 FAMTELHHGSNVQGLQTTATFDPITDEFIINTPNDGAIKWWIGNAAVHGKFATVFAKLVL 242

Query: 242 PTHDSKKIADMGVHAFIVPIRDLKSHKTLPGIEIHDCGHKIGLNGVDNGALRFRSVRIPR 301
           PTHDS+K ADMGVHAFIVPIRDLKSHKTL GIEIHDCGHK+GLNGVDNGALRFRSVRIPR
Sbjct: 243 PTHDSRKTADMGVHAFIVPIRDLKSHKTLAGIEIHDCGHKVGLNGVDNGALRFRSVRIPR 302

Query: 302 DNLLDRFGEVSRDGKYKSSLPSISKRFAATLGELVGGRVGLAYSSASVLKVASTIAIRYS 361
           DNLLDRFGEVSRDGKYKSSLPSI+KRFAATLGELVGGRVGLAYSSASVLK+ASTIAIRYS
Sbjct: 303 DNLLDRFGEVSRDGKYKSSLPSINKRFAATLGELVGGRVGLAYSSASVLKIASTIAIRYS 362

Query: 362 LLRQQFGPLKQPEVSILDYQSQQHKLMPMLASTYAFHFATLQLVEKYAQMKKTHDEQL-A 421
           LLRQQFGP KQPEVSILDYQSQQHKLMPMLASTYAFHF+T+QLVEKYAQMKKTHDE+L  
Sbjct: 363 LLRQQFGPPKQPEVSILDYQSQQHKLMPMLASTYAFHFSTMQLVEKYAQMKKTHDEELVG 422

Query: 422 EIH--------------------------------------------------------- 481
           ++H                                                         
Sbjct: 423 DVHALSAGLKAYVTSYTAKSLSTCREACGGHGYAVVNRFGTLRNDHDIFQTFEGDNTVLL 482

Query: 482 ------LLKQYKEKFEGGTLTVTWNYLRESMSTYLSQPNPVTARWEIPN----------- 541
                 LLKQYKEKF+GGTL VTWNYLRESM+TYLSQPNPVTARWE  +           
Sbjct: 483 QQVAAYLLKQYKEKFQGGTLAVTWNYLRESMNTYLSQPNPVTARWESADHLRDPKFQLDA 542

Query: 542 -----VSITSSAAVRLRKHTKTLGSFGAWNRCLNHLLTLAESHIESVILAKFIEAVQSCP 601
                  +  S AVRLRKHTK LGSFGAWNRCLNHLLTLAESHIESVILA+FIEAVQ CP
Sbjct: 543 FQYRTSRLLQSVAVRLRKHTKNLGSFGAWNRCLNHLLTLAESHIESVILAQFIEAVQRCP 602

Query: 602 NASTQATLKLVCDLYALDRIWNDIGTYRNVDYVAPNKAKAIHKLTEYLCFQVRNIAKELV 607
           NA+TQATLKLVCDLYALDRIWNDIGTYRNVDYVAPNKAKAIHKLTEYLCFQVRNIA+ELV
Sbjct: 603 NANTQATLKLVCDLYALDRIWNDIGTYRNVDYVAPNKAKAIHKLTEYLCFQVRNIAQELV 662

BLAST of Sgr020616 vs. NCBI nr
Match: XP_022929316.1 (acyl-coenzyme A oxidase, peroxisomal [Cucurbita moschata] >XP_022929317.1 acyl-coenzyme A oxidase, peroxisomal [Cucurbita moschata])

HSP 1 Score: 1057.4 bits (2733), Expect = 7.8e-305
Identity = 540/687 (78.60%), Postives = 576/687 (83.84%), Query Frame = 0

Query: 2   SSGEPNQMAEDESQAVSRRIQRLSFHLTPIPLPCGGSHQLEMLTCSA--AKAKLEVDIAS 61
           S GEPN+ AEDESQA +RRI+RLS HLTPIPL    S  +EM TC A  AKAK+EVD+ S
Sbjct: 3   SPGEPNRTAEDESQAAARRIERLSLHLTPIPL--DDSQGVEMETCGAGKAKAKIEVDMGS 62

Query: 62  LSLYMRGKHREIQERVFEYFNSRPELQTPVEISMSDHRELCMKQLIGLVREAGIRPYRYV 121
           LSLYMR KHRE+QERVFEYFNSRPELQTPV ISM+DHR+LCMKQL+GLVREAGIRP+R+V
Sbjct: 63  LSLYMRKKHREVQERVFEYFNSRPELQTPVGISMADHRDLCMKQLVGLVREAGIRPFRFV 122

Query: 122 NEDPAKYFAIMEAVGSIDVSFAIKMGVQFSLWGGSVLNLGTKKHRDKFFDGIDNVDYPGC 181
           NEDPAKYFAIMEAVGS+DVS AIKMGVQFSLWGGSV+NLGTKKHRD+FFDGIDNVDYPGC
Sbjct: 123 NEDPAKYFAIMEAVGSVDVSLAIKMGVQFSLWGGSVINLGTKKHRDRFFDGIDNVDYPGC 182

Query: 182 FAMTELHHGSNVQGLQTTATFDPITDEFIINTPNDGAIKWWIGNAAVHGKFATVFAKLVL 241
           FAMTELHHGSNVQGLQTTATFDPITDEFIINTPNDGAIKWWIGNAAVHGKFATVFAKLVL
Sbjct: 183 FAMTELHHGSNVQGLQTTATFDPITDEFIINTPNDGAIKWWIGNAAVHGKFATVFAKLVL 242

Query: 242 PTHDSKKIADMGVHAFIVPIRDLKSHKTLPGIEIHDCGHKIGLNGVDNGALRFRSVRIPR 301
           PTHDS+K ADMGVHAFIVPIRDLKSHKTLPGIEIHDCGHK+GLNGVDNGALRFR VRIPR
Sbjct: 243 PTHDSRKTADMGVHAFIVPIRDLKSHKTLPGIEIHDCGHKVGLNGVDNGALRFRLVRIPR 302

Query: 302 DNLLDRFGEVSRDGKYKSSLPSISKRFAATLGELVGGRVGLAYSSASVLKVASTIAIRYS 361
           DNLLDRFGEVSRDGKYKSSLPSI+KRFAATLGELVGGRVGLAYSSASVLK+ASTIAIRYS
Sbjct: 303 DNLLDRFGEVSRDGKYKSSLPSINKRFAATLGELVGGRVGLAYSSASVLKIASTIAIRYS 362

Query: 362 LLRQQFGPLKQPEVSILDYQSQQHKLMPMLASTYAFHFATLQLVEKYAQMKKTHDEQL-A 421
           LLRQQFGP KQPEVSILDYQSQQHKLMPMLASTYAFHF+T+QLVEKYAQMKKTHDE+L  
Sbjct: 363 LLRQQFGPPKQPEVSILDYQSQQHKLMPMLASTYAFHFSTMQLVEKYAQMKKTHDEELVG 422

Query: 422 EIH--------------------------------------------------------- 481
           ++H                                                         
Sbjct: 423 DVHALSAGLKAYITSYTAKSLSTCREACGGHGYAVVNRFGTLRNDHDIFQTFEGDNTVLL 482

Query: 482 ------LLKQYKEKFEGGTLTVTWNYLRESMSTYLSQPNPVTARWEIPN----------- 541
                 LLKQYKEKF+GGTLTVTWNYLRESM+TYLSQPNPVTARWE  +           
Sbjct: 483 QQVAAYLLKQYKEKFQGGTLTVTWNYLRESMNTYLSQPNPVTARWESADHLRDPKFQLDA 542

Query: 542 -----VSITSSAAVRLRKHTKTLGSFGAWNRCLNHLLTLAESHIESVILAKFIEAVQSCP 601
                  +  S AVRLRKHTK LGSFGAWNRCLNHLLTLAESHIESVILA+FIEAVQ CP
Sbjct: 543 FQYRTSRLLQSVAVRLRKHTKNLGSFGAWNRCLNHLLTLAESHIESVILAQFIEAVQRCP 602

Query: 602 NASTQATLKLVCDLYALDRIWNDIGTYRNVDYVAPNKAKAIHKLTEYLCFQVRNIAKELV 607
           NA+TQATLKLVCDLYALDRIWNDIGTYRNVDYVAPNKAKAIHKLTEYLCFQVRNIA+ELV
Sbjct: 603 NANTQATLKLVCDLYALDRIWNDIGTYRNVDYVAPNKAKAIHKLTEYLCFQVRNIAQELV 662

BLAST of Sgr020616 vs. NCBI nr
Match: XP_022136505.1 (acyl-coenzyme A oxidase, peroxisomal [Momordica charantia])

HSP 1 Score: 1055.8 bits (2729), Expect = 2.3e-304
Identity = 542/686 (79.01%), Postives = 572/686 (83.38%), Query Frame = 0

Query: 1   MSSGEPNQMAEDESQAVSRRIQRLSFHLTPIPLPCGGSHQLEMLTCSAAKAKLEVDIASL 60
           MSSGE N+ AEDESQA+SRRI+RLS H+ PIP+P  GS QL+MLTC AAKAKLEVD ASL
Sbjct: 1   MSSGESNRTAEDESQAISRRIERLSLHMAPIPVPGDGS-QLQMLTCGAAKAKLEVDTASL 60

Query: 61  SLYMRGKHREIQERVFEYFNSRPELQTPVEISMSDHRELCMKQLIGLVREAGIRPYRYVN 120
           S YMRGKHREIQERVFEYFNSRPEL TPVEISM+DHRELCMKQLI LVREAGIRP R+VN
Sbjct: 61  SQYMRGKHREIQERVFEYFNSRPELHTPVEISMADHRELCMKQLIALVREAGIRPLRFVN 120

Query: 121 EDPAKYFAIMEAVGSIDVSFAIKMGVQFSLWGGSVLNLGTKKHRDKFFDGIDNVDYPGCF 180
           +DPAKYFAIMEAVGSIDVS AIKMGVQFSLWGGSVLNLGTKKHRDKFFDGIDN+DYPGCF
Sbjct: 121 DDPAKYFAIMEAVGSIDVSLAIKMGVQFSLWGGSVLNLGTKKHRDKFFDGIDNLDYPGCF 180

Query: 181 AMTELHHGSNVQGLQTTATFDPITDEFIINTPNDGAIKWWIGNAAVHGKFATVFAKLVLP 240
           AMTELHHGSNVQGLQTTATFDPITDEFIINTPNDGAIKWWIGNAAVHGKFATVFAKL+LP
Sbjct: 181 AMTELHHGSNVQGLQTTATFDPITDEFIINTPNDGAIKWWIGNAAVHGKFATVFAKLILP 240

Query: 241 THDSKKIADMGVHAFIVPIRDLKSHKTLPGIEIHDCGHKIGLNGVDNGALRFRSVRIPRD 300
           T+D+KK ADMGVHAFIVPIRDLKSHKTLPGIEIHDCGHK+GLNGVDNGALRF SVRIPRD
Sbjct: 241 TYDAKKTADMGVHAFIVPIRDLKSHKTLPGIEIHDCGHKVGLNGVDNGALRFSSVRIPRD 300

Query: 301 NLLDRFGEVSRDGKYKSSLPSISKRFAATLGELVGGRVGLAYSSASVLKVASTIAIRYSL 360
           NLLDRFGEVSRDGKYKSSLPSIS+RFAATLGELVGGRV LAYSS SVLK+ASTIAIRYSL
Sbjct: 301 NLLDRFGEVSRDGKYKSSLPSISRRFAATLGELVGGRVALAYSSVSVLKIASTIAIRYSL 360

Query: 361 LRQQFGPLKQPEVSILDYQSQQHKLMPMLASTYAFHFATLQLVEKYAQMKKTHDEQL-AE 420
           LRQQFGP KQPEVSILDYQSQQHKLMPMLAS YAFHFATLQLVEKYA MKKT+DE+L  +
Sbjct: 361 LRQQFGPPKQPEVSILDYQSQQHKLMPMLASNYAFHFATLQLVEKYALMKKTNDEELVGD 420

Query: 421 IH---------------------------------------------------------- 480
           +H                                                          
Sbjct: 421 VHALSAGLKAYVTSYTAKSLSTCREACGGHGYAAVNRFGSLRNDHDIFQTFEGDNTVLLQ 480

Query: 481 -----LLKQYKEKFEGGTLTVTWNYLRESMSTYLSQPNPVTARWEIPN------------ 540
                LLKQYKEKFEGGTL VTWNYLRESM+TYLSQPNPVTARWE  +            
Sbjct: 481 QVAAYLLKQYKEKFEGGTLAVTWNYLRESMNTYLSQPNPVTARWESADHLRDPKFQLDAF 540

Query: 541 ----VSITSSAAVRLRKHTKTLGSFGAWNRCLNHLLTLAESHIESVILAKFIEAVQSCPN 600
                 +  S AVRLRKHTKTLGSFGAWNRCLNHLLTLAESHIESVILA+FI+AVQ CPN
Sbjct: 541 QYRTSRLLQSVAVRLRKHTKTLGSFGAWNRCLNHLLTLAESHIESVILAQFIKAVQRCPN 600

Query: 601 ASTQATLKLVCDLYALDRIWNDIGTYRNVDYVAPNKAKAIHKLTEYLCFQVRNIAKELVD 607
           A+TQATLKL+CDLYALDRIWNDIGTYRNVDYVAPNKAKAIHKL EYL FQVRNIAKELVD
Sbjct: 601 ANTQATLKLLCDLYALDRIWNDIGTYRNVDYVAPNKAKAIHKLAEYLSFQVRNIAKELVD 660

BLAST of Sgr020616 vs. ExPASy Swiss-Prot
Match: O64894 (Acyl-coenzyme A oxidase, peroxisomal OS=Cucurbita maxima OX=3661 GN=Acx PE=1 SV=1)

HSP 1 Score: 1058.1 bits (2735), Expect = 6.0e-308
Identity = 540/687 (78.60%), Postives = 578/687 (84.13%), Query Frame = 0

Query: 2   SSGEPNQMAEDESQAVSRRIQRLSFHLTPIPLPCGGSHQLEMLTCSA--AKAKLEVDIAS 61
           S GEPN+ AEDESQA +RRI+RLS HLTPIPL    S  +EM TC+A  AKAK+EVD+ S
Sbjct: 3   SPGEPNRTAEDESQAAARRIERLSLHLTPIPL--DDSQGVEMETCAAGKAKAKIEVDMGS 62

Query: 62  LSLYMRGKHREIQERVFEYFNSRPELQTPVEISMSDHRELCMKQLIGLVREAGIRPYRYV 121
           LSLYMRGKHREIQERVFEYFNSRPELQTPV ISM+DHRELCMKQL+GLVREAGIRP+R+V
Sbjct: 63  LSLYMRGKHREIQERVFEYFNSRPELQTPVGISMADHRELCMKQLVGLVREAGIRPFRFV 122

Query: 122 NEDPAKYFAIMEAVGSIDVSFAIKMGVQFSLWGGSVLNLGTKKHRDKFFDGIDNVDYPGC 181
           NEDPAKYFAIMEAVGS+DVS AIKMGVQFSLWGGSV+NLGTKKHRD+FFDGIDNVDYPGC
Sbjct: 123 NEDPAKYFAIMEAVGSVDVSLAIKMGVQFSLWGGSVINLGTKKHRDRFFDGIDNVDYPGC 182

Query: 182 FAMTELHHGSNVQGLQTTATFDPITDEFIINTPNDGAIKWWIGNAAVHGKFATVFAKLVL 241
           FAMTELHHGSNVQGLQTTATFDPITDEFIINTPNDGAIKWWIGNAAVHGKFATVFAKLVL
Sbjct: 183 FAMTELHHGSNVQGLQTTATFDPITDEFIINTPNDGAIKWWIGNAAVHGKFATVFAKLVL 242

Query: 242 PTHDSKKIADMGVHAFIVPIRDLKSHKTLPGIEIHDCGHKIGLNGVDNGALRFRSVRIPR 301
           PTHDS+K ADMGVHAFIVPIRDLKSHKTLPGIEIHDCGHK+GLNGVDNGALRFRSVRIPR
Sbjct: 243 PTHDSRKTADMGVHAFIVPIRDLKSHKTLPGIEIHDCGHKVGLNGVDNGALRFRSVRIPR 302

Query: 302 DNLLDRFGEVSRDGKYKSSLPSISKRFAATLGELVGGRVGLAYSSASVLKVASTIAIRYS 361
           DNLL+RFGEVSRDGKYKSSLPSI+KRFAATLGELVGGRVGLAYSSASVLK+ASTIAIRYS
Sbjct: 303 DNLLNRFGEVSRDGKYKSSLPSINKRFAATLGELVGGRVGLAYSSASVLKIASTIAIRYS 362

Query: 362 LLRQQFGPLKQPEVSILDYQSQQHKLMPMLASTYAFHFATLQLVEKYAQMKKTHDEQL-A 421
           LLRQQFGP KQPEVSILDYQSQQHKLMPMLASTYAFHF+T+QLVEKYAQMKKTHDE+L  
Sbjct: 363 LLRQQFGPPKQPEVSILDYQSQQHKLMPMLASTYAFHFSTMQLVEKYAQMKKTHDEELVG 422

Query: 422 EIH--------------------------------------------------------- 481
           ++H                                                         
Sbjct: 423 DVHALSAGLKAYVTSYTAKSLSTCREACGGHGYAVVNRFGTLRNDHDIFQTFEGDNTVLL 482

Query: 482 ------LLKQYKEKFEGGTLTVTWNYLRESMSTYLSQPNPVTARWEIPN----------- 541
                 LLKQY+EKF+GGTL VTWNYLRESM+TYLSQPNPVTARWE  +           
Sbjct: 483 QQVAAYLLKQYQEKFQGGTLAVTWNYLRESMNTYLSQPNPVTARWESADHLRDPKFQLDA 542

Query: 542 -----VSITSSAAVRLRKHTKTLGSFGAWNRCLNHLLTLAESHIESVILAKFIEAVQSCP 601
                  +  S AVRLRKHTK LGSFGAWNRCLNHLLTLAESHIESVILA+FIE+VQ CP
Sbjct: 543 FQYRTSRLLQSVAVRLRKHTKNLGSFGAWNRCLNHLLTLAESHIESVILAQFIESVQRCP 602

Query: 602 NASTQATLKLVCDLYALDRIWNDIGTYRNVDYVAPNKAKAIHKLTEYLCFQVRNIAKELV 607
           NA+TQATLKLVCDLYALDRIWNDIGTYRNVDYVAPNKAKAIHKLTEYLCFQVRNIA+ELV
Sbjct: 603 NANTQATLKLVCDLYALDRIWNDIGTYRNVDYVAPNKAKAIHKLTEYLCFQVRNIAQELV 662

BLAST of Sgr020616 vs. ExPASy Swiss-Prot
Match: O65201 (Acyl-coenzyme A oxidase 2, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=ACX2 PE=1 SV=2)

HSP 1 Score: 953.7 bits (2464), Expect = 1.6e-276
Identity = 477/686 (69.53%), Postives = 545/686 (79.45%), Query Frame = 0

Query: 2   SSGEPNQMAEDESQAV--SRRIQRLSFHLTPIPLPCGGSHQLEMLTCSAAKAKLEVDIAS 61
           S  E N M E+ES  +  +RRIQRLS HL+P   P      ++  TCSA   KL+V+  +
Sbjct: 3   SRREKNPMTEEESDGLIAARRIQRLSLHLSPSLTPSPSLPLVQTETCSARSKKLDVNGEA 62

Query: 62  LSLYMRGKHREIQERVFEYFNSRPELQTPVEISMSDHRELCMKQLIGLVREAGIRPYRYV 121
           LSLYMRGKH +IQE++F++FNSRP+LQTP+EIS  DHRELCM QLIGLVREAG+RP+RYV
Sbjct: 63  LSLYMRGKHIDIQEKIFDFFNSRPDLQTPIEISKDDHRELCMNQLIGLVREAGVRPFRYV 122

Query: 122 NEDPAKYFAIMEAVGSIDVSFAIKMGVQFSLWGGSVLNLGTKKHRDKFFDGIDNVDYPGC 181
            +DP KYFAIMEAVGS+D+S  IKMGVQ+SLWGGSV+NLGTKKHRDK+FDGIDN+DY GC
Sbjct: 123 ADDPEKYFAIMEAVGSVDMSLGIKMGVQYSLWGGSVINLGTKKHRDKYFDGIDNLDYTGC 182

Query: 182 FAMTELHHGSNVQGLQTTATFDPITDEFIINTPNDGAIKWWIGNAAVHGKFATVFAKLVL 241
           FAMTELHHGSNVQGLQTTATFDP+ DEF+I+TPNDGAIKWWIGNAAVHGKFATVFA+L+L
Sbjct: 183 FAMTELHHGSNVQGLQTTATFDPLKDEFVIDTPNDGAIKWWIGNAAVHGKFATVFARLIL 242

Query: 242 PTHDSKKIADMGVHAFIVPIRDLKSHKTLPGIEIHDCGHKIGLNGVDNGALRFRSVRIPR 301
           PTHDSK ++DMGVHAFIVPIRD+K+H+TLPG+EI DCGHK+GLNGVDNGALRFRSVRIPR
Sbjct: 243 PTHDSKGVSDMGVHAFIVPIRDMKTHQTLPGVEIQDCGHKVGLNGVDNGALRFRSVRIPR 302

Query: 302 DNLLDRFGEVSRDGKYKSSLPSISKRFAATLGELVGGRVGLAYSSASVLKVASTIAIRYS 361
           DNLL+RFG+VSRDG Y SSLP+I+KRF ATLGELVGGRVGLAY+S  VLK+++TIAIRYS
Sbjct: 303 DNLLNRFGDVSRDGTYTSSLPTINKRFGATLGELVGGRVGLAYASVGVLKISATIAIRYS 362

Query: 362 LLRQQFGPLKQPEVSILDYQSQQHKLMPMLASTYAFHFATLQLVEKYAQMKKTHDEQL-A 421
           LLRQQFGP KQPEVSILDYQSQQHKLMPMLASTYA+HFAT+ LVEKY++MKKTHDEQL A
Sbjct: 363 LLRQQFGPPKQPEVSILDYQSQQHKLMPMLASTYAYHFATVYLVEKYSEMKKTHDEQLVA 422

Query: 422 EIH--------------------------------------------------------- 481
           ++H                                                         
Sbjct: 423 DVHALSAGLKSYVTSYTAKALSVCREACGGHGYAAVNRFGSLRNDHDIFQTFEGDNTVLL 482

Query: 482 ------LLKQYKEKFEGGTLTVTWNYLRESMSTYLSQPNPVTARWE-------------- 541
                 LLK+YKEKF+GGTLTVTW+YLRESM+TYLSQPNPVTARWE              
Sbjct: 483 QQVAADLLKRYKEKFQGGTLTVTWSYLRESMNTYLSQPNPVTARWEGEDHLRDPKFQLDA 542

Query: 542 --IPNVSITSSAAVRLRKHTKTLGSFGAWNRCLNHLLTLAESHIESVILAKFIEAVQSCP 601
                  +  + A RL+KH+KTLG FGAWNRCLNHLLTLAESHIE+VILAKFIEAV++CP
Sbjct: 543 FRYRTSRLLQNVAARLQKHSKTLGGFGAWNRCLNHLLTLAESHIETVILAKFIEAVKNCP 602

Query: 602 NASTQATLKLVCDLYALDRIWNDIGTYRNVDYVAPNKAKAIHKLTEYLCFQVRNIAKELV 606
           + S +A LKL CDLYALDRIW DIGTYRNVDYVAPNKAKAIHKLTEYL FQVRN+AKELV
Sbjct: 603 DPSAKAALKLACDLYALDRIWKDIGTYRNVDYVAPNKAKAIHKLTEYLSFQVRNVAKELV 662

BLAST of Sgr020616 vs. ExPASy Swiss-Prot
Match: Q9DBS4 (Acyl-coenzyme A oxidase-like protein OS=Mus musculus OX=10090 GN=Acoxl PE=1 SV=1)

HSP 1 Score: 266.9 bits (681), Expect = 8.9e-70
Identity = 183/541 (33.83%), Postives = 262/541 (48.43%), Query Frame = 0

Query: 131 EAVGSIDVSFAIKMGVQFSLWGGSVLNLGTKKHRDKFFDGIDNVDYPGCFAMTELHHGSN 190
           E +  +DVS +IK G+ F L+GG++ NLG+ +H  K+F  +    Y G FAMTE  HGSN
Sbjct: 67  EVLCMVDVSMSIKCGILFLLFGGAISNLGSPEHVTKWFWPLKEQKYTGMFAMTERGHGSN 126

Query: 191 VQGLQTTATFDPITDEFIINTPNDGAIKWWIGNAAVHGKFATVFAKLVLPTHDSKKIADM 250
           V+G+QT ATFD    EF+I+ P + A K +IGN A+HG +A VFA+L++      +    
Sbjct: 127 VRGIQTEATFDLDNQEFVIDMPCENAHKMYIGN-AMHGNYAAVFAQLII------EGKSQ 186

Query: 251 GVHAFIVPIRDLKSHKTLPGIEIHDCGHKIGLNGVDNGALRFRSVRIPRDNLLDRFGEVS 310
           G H FIVPIRD ++    PG+   D  HK G+NGVDNG L F  VRIPR+NLLD+FG V+
Sbjct: 187 GPHCFIVPIRD-ENGNLYPGVTAIDMMHKEGMNGVDNGILIFDKVRIPRENLLDKFGSVT 246

Query: 311 RDGKYKSSLPSISKRFAATLGELVGGRVGLAYSSASVLKVASTIAIRYSLLRQQFGPLKQ 370
            DG+Y S + S + RF A L  L   R+ + + +   +K+   IAIRYS  R+QFGP  +
Sbjct: 247 PDGQYHSPIQSKNARFNAILATLTPSRLAVTFQALGAMKLGLMIAIRYSHSRRQFGPKDK 306

Query: 371 PEVSILDYQSQQHKLMPMLASTYAFHFATLQ----LVEKYAQMKK-THDEQLAEIHL-LK 430
            EV I+++Q Q  +LM  LA+  A  F +      L E   Q +  T+   L  +   LK
Sbjct: 307 EEVKIIEHQMQALRLMSHLATALAVTFTSRHADDILDEDIFQGRALTNSRSLQALMAGLK 366

Query: 431 QYKE------------------------------------KFEGGTLTVTWNYLRESMST 490
            Y                                       FEG  + +     RE ++ 
Sbjct: 367 AYSTWETVSCLQDCRECTGGMGYMMETRISDLKCDTDVFVTFEGDNVVMLQVVARELLAQ 426

Query: 491 YLSQPNPVTARWEIPNVSITSSAAVR---LRKHTKTLGS--------------------- 550
           Y  Q         I N + T+   +R   L  +T T+G                      
Sbjct: 427 YSKQHKKNLLLGVIQNWTATAGDKLRTSFLAFNTDTVGCLAFLLKAVNFRERVLQRSLVS 486

Query: 551 -------------FGAWNRCLNHLLTLAESHIESVILAKFIEAVQSCPNASTQATLKLVC 593
                        F AWN C++H+ +L+ +HI  V L +F  AV+ CPN   QA L   C
Sbjct: 487 RIYYKVVTKKGDFFSAWNSCMHHVTSLSLAHIHRVALEQFTTAVRQCPNREDQALLMKFC 546

BLAST of Sgr020616 vs. ExPASy Swiss-Prot
Match: P0CZ23 (Acyl-coenzyme A oxidase 3, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=ACX3 PE=1 SV=1)

HSP 1 Score: 261.2 bits (666), Expect = 4.9e-68
Identity = 151/352 (42.90%), Postives = 207/352 (58.81%), Query Frame = 0

Query: 92  SMSDHRELCMKQLIGLVREAGIRPYRYVN--EDPAKYFAIMEAVGSIDVSFAIKMGVQFS 151
           +M   RE+ MK++  L+     + +      E   +  A++E  G  D S +IK+GV F 
Sbjct: 96  TMEQQREITMKRIWYLLENGVFKGWLTETGPEAELRKLALLEVCGIYDHSVSIKVGVHFF 155

Query: 152 LWGGSVLNLGTKKHRDKFFDGIDNVDYPGCFAMTELHHGSNVQGLQTTATFDPITDEFII 211
           LWG +V   GTK+H +K+    ++    GCFAMTEL HGSNV+G++T  T+DP T+EF+I
Sbjct: 156 LWGNAVKFFGTKRHHEKWLKNTEDYVVKGCFAMTELGHGSNVRGIETVTTYDPKTEEFVI 215

Query: 212 NTPNDGAIKWWIGNAAVHGKFATVFAKLVLPTHDSKKIADMGVHAFIVPIRDLKSHKTLP 271
           NTP + A K+WIG AA H     VF++L +         + GVHAFI  IRD +     P
Sbjct: 216 NTPCESAQKYWIGGAANHATHTIVFSQLHI------NGTNQGVHAFIAQIRD-QDGSICP 275

Query: 272 GIEIHDCGHKIGLNGVDNGALRFRSVRIPRDNLLDRFGEVSRDGKYKSSLPSISKRFAAT 331
            I I DCGHKIGLNGVDNG + F ++RIPR+NLL+   +VS DGKY SS+    +RF A 
Sbjct: 276 NIRIADCGHKIGLNGVDNGRIWFDNLRIPRENLLNAVADVSSDGKYVSSIKDPDQRFGAF 335

Query: 332 LGELVGGRVGLAYSSASVLKVASTIAIRYSLLRQQFG-PLKQPEVSILDYQSQQHKLMPM 391
           +  L  GRV +A S+    KV  +IAIRYSL R+ F      PEV +LDY S Q +L+P+
Sbjct: 336 MAPLTSGRVTIASSAIYSAKVGLSIAIRYSLSRRAFSVTANGPEVLLLDYPSHQRRLLPL 395

Query: 392 LASTYAFHFATLQLVEKYAQMKKTHDEQLAEIHLLKQYKEKFEGGTLTVTWN 441
           LA TYA  FA  +L  K   +K+T +   A IH++        G    +TW+
Sbjct: 396 LAKTYAMSFAANEL--KMIYVKRTPETNKA-IHVVS------SGFKAVLTWH 431

BLAST of Sgr020616 vs. ExPASy Swiss-Prot
Match: Q9LMI7 (Putative acyl-coenzyme A oxidase 3.2, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=ACX3.2 PE=3 SV=1)

HSP 1 Score: 260.4 bits (664), Expect = 8.4e-68
Identity = 148/355 (41.69%), Postives = 205/355 (57.75%), Query Frame = 0

Query: 92  SMSDHRELCMKQLIGLVREAGIRPYRYVN--EDPAKYFAIMEAVGSIDVSFAIKMGVQFS 151
           +M   R++ MK++  L+ +   + +      E   K FA+ E  G  D S + K+GV F 
Sbjct: 96  TMEQQRQISMKRIFYLLEKGVFQGWLTETGPEAELKKFALYEVCGIYDYSLSAKLGVHFL 155

Query: 152 LWGGSVLNLGTKKHRDKFFDGIDNVDYPGCFAMTELHHGSNVQGLQTTATFDPITDEFII 211
           LWG +V   GTK+H +K+    ++    GCFAMTEL HG+NV+G++T  T+DP T+EF+I
Sbjct: 156 LWGNAVKFFGTKRHHEKWLKDTEDYVVKGCFAMTELGHGTNVRGIETVTTYDPTTEEFVI 215

Query: 212 NTPNDGAIKWWIGNAAVHGKFATVFAKLVLPTHDSKKIADMGVHAFIVPIRDLKSHKTLP 271
           NTP + A K+WIG AA H   A V ++L      S    + G+H FI  IRD     T P
Sbjct: 216 NTPCESAQKYWIGEAANHANHAIVISQL------SMNGTNQGIHVFIAQIRD-HDGNTCP 275

Query: 272 GIEIHDCGHKIGLNGVDNGALRFRSVRIPRDNLLDRFGEVSRDGKYKSSLPSISKRFAAT 331
            + I DCGHKIGLNGVDNG + F ++RIPR+NLL+   +V  DGKY SS+    +RF A 
Sbjct: 276 NVRIADCGHKIGLNGVDNGRIWFDNLRIPRENLLNSVADVLADGKYVSSIKDPDQRFGAF 335

Query: 332 LGELVGGRVGLAYSSASVLKVASTIAIRYSLLRQQFG-PLKQPEVSILDYQSQQHKLMPM 391
           L  L  GRV +A S+    K+   +AIRYSL R+ F      PEV +LDY S Q +L+P+
Sbjct: 336 LAPLTSGRVTIASSAIYSAKLGLAVAIRYSLSRRAFSVAANGPEVLLLDYPSHQRRLLPL 395

Query: 392 LASTYAFHFATLQLVEKYAQMKKTHDEQLAEIHLLKQYKEKFEGGTLTVTWNYLR 444
           LA TYA  FA   L  K   +K+T +   A IH++        G    +TW+ +R
Sbjct: 396 LAKTYAMSFAVNDL--KMIYVKRTPETNKA-IHVVS------SGFKAVLTWHNMR 434

BLAST of Sgr020616 vs. ExPASy TrEMBL
Match: A0A6J1J1U1 (Acyl-coenzyme A oxidase OS=Cucurbita maxima OX=3661 GN=LOC111482638 PE=3 SV=1)

HSP 1 Score: 1058.1 bits (2735), Expect = 2.2e-305
Identity = 540/687 (78.60%), Postives = 578/687 (84.13%), Query Frame = 0

Query: 2   SSGEPNQMAEDESQAVSRRIQRLSFHLTPIPLPCGGSHQLEMLTCSA--AKAKLEVDIAS 61
           S GEPN+ AEDESQA +RRI+RLS HLTPIPL    S  +EM TC+A  AKAK+EVD+ S
Sbjct: 3   SPGEPNRTAEDESQAAARRIERLSLHLTPIPL--DDSQGVEMETCAAGKAKAKIEVDMGS 62

Query: 62  LSLYMRGKHREIQERVFEYFNSRPELQTPVEISMSDHRELCMKQLIGLVREAGIRPYRYV 121
           LSLYMRGKHREIQERVFEYFNSRPELQTPV ISM+DHRELCMKQL+GLVREAGIRP+R+V
Sbjct: 63  LSLYMRGKHREIQERVFEYFNSRPELQTPVGISMADHRELCMKQLVGLVREAGIRPFRFV 122

Query: 122 NEDPAKYFAIMEAVGSIDVSFAIKMGVQFSLWGGSVLNLGTKKHRDKFFDGIDNVDYPGC 181
           NEDPAKYFAIMEAVGS+DVS AIKMGVQFSLWGGSV+NLGTKKHRD+FFDGIDNVDYPGC
Sbjct: 123 NEDPAKYFAIMEAVGSVDVSLAIKMGVQFSLWGGSVINLGTKKHRDRFFDGIDNVDYPGC 182

Query: 182 FAMTELHHGSNVQGLQTTATFDPITDEFIINTPNDGAIKWWIGNAAVHGKFATVFAKLVL 241
           FAMTELHHGSNVQGLQTTATFDPITDEFIINTPNDGAIKWWIGNAAVHGKFATVFAKLVL
Sbjct: 183 FAMTELHHGSNVQGLQTTATFDPITDEFIINTPNDGAIKWWIGNAAVHGKFATVFAKLVL 242

Query: 242 PTHDSKKIADMGVHAFIVPIRDLKSHKTLPGIEIHDCGHKIGLNGVDNGALRFRSVRIPR 301
           PTHDS+K ADMGVHAFIVPIRDLKSHKTLPGIEIHDCGHK+GLNGVDNGALRFRSVRIPR
Sbjct: 243 PTHDSRKTADMGVHAFIVPIRDLKSHKTLPGIEIHDCGHKVGLNGVDNGALRFRSVRIPR 302

Query: 302 DNLLDRFGEVSRDGKYKSSLPSISKRFAATLGELVGGRVGLAYSSASVLKVASTIAIRYS 361
           DNLL+RFGEVSRDGKYKSSLPSI+KRFAATLGELVGGRVGLAYSSASVLK+ASTIAIRYS
Sbjct: 303 DNLLNRFGEVSRDGKYKSSLPSINKRFAATLGELVGGRVGLAYSSASVLKIASTIAIRYS 362

Query: 362 LLRQQFGPLKQPEVSILDYQSQQHKLMPMLASTYAFHFATLQLVEKYAQMKKTHDEQL-A 421
           LLRQQFGP KQPEVSILDYQSQQHKLMPMLASTYAFHF+T+QLVEKYAQMKKTHDE+L  
Sbjct: 363 LLRQQFGPPKQPEVSILDYQSQQHKLMPMLASTYAFHFSTMQLVEKYAQMKKTHDEELVG 422

Query: 422 EIH--------------------------------------------------------- 481
           ++H                                                         
Sbjct: 423 DVHALSAGLKAYVTSYTAKSLSTCREACGGHGYAVVNRFGTLRNDHDIFQTFEGDNTVLL 482

Query: 482 ------LLKQYKEKFEGGTLTVTWNYLRESMSTYLSQPNPVTARWEIPN----------- 541
                 LLKQY+EKF+GGTL VTWNYLRESM+TYLSQPNPVTARWE  +           
Sbjct: 483 QQVAAYLLKQYQEKFQGGTLAVTWNYLRESMNTYLSQPNPVTARWESADHLRDPKFQLDA 542

Query: 542 -----VSITSSAAVRLRKHTKTLGSFGAWNRCLNHLLTLAESHIESVILAKFIEAVQSCP 601
                  +  S AVRLRKHTK LGSFGAWNRCLNHLLTLAESHIESVILA+FIE+VQ CP
Sbjct: 543 FQYRTSRLLQSVAVRLRKHTKNLGSFGAWNRCLNHLLTLAESHIESVILAQFIESVQRCP 602

Query: 602 NASTQATLKLVCDLYALDRIWNDIGTYRNVDYVAPNKAKAIHKLTEYLCFQVRNIAKELV 607
           NA+TQATLKLVCDLYALDRIWNDIGTYRNVDYVAPNKAKAIHKLTEYLCFQVRNIA+ELV
Sbjct: 603 NANTQATLKLVCDLYALDRIWNDIGTYRNVDYVAPNKAKAIHKLTEYLCFQVRNIAQELV 662

BLAST of Sgr020616 vs. ExPASy TrEMBL
Match: A0A6J1EMG5 (Acyl-coenzyme A oxidase OS=Cucurbita moschata OX=3662 GN=LOC111435934 PE=3 SV=1)

HSP 1 Score: 1057.4 bits (2733), Expect = 3.8e-305
Identity = 540/687 (78.60%), Postives = 576/687 (83.84%), Query Frame = 0

Query: 2   SSGEPNQMAEDESQAVSRRIQRLSFHLTPIPLPCGGSHQLEMLTCSA--AKAKLEVDIAS 61
           S GEPN+ AEDESQA +RRI+RLS HLTPIPL    S  +EM TC A  AKAK+EVD+ S
Sbjct: 3   SPGEPNRTAEDESQAAARRIERLSLHLTPIPL--DDSQGVEMETCGAGKAKAKIEVDMGS 62

Query: 62  LSLYMRGKHREIQERVFEYFNSRPELQTPVEISMSDHRELCMKQLIGLVREAGIRPYRYV 121
           LSLYMR KHRE+QERVFEYFNSRPELQTPV ISM+DHR+LCMKQL+GLVREAGIRP+R+V
Sbjct: 63  LSLYMRKKHREVQERVFEYFNSRPELQTPVGISMADHRDLCMKQLVGLVREAGIRPFRFV 122

Query: 122 NEDPAKYFAIMEAVGSIDVSFAIKMGVQFSLWGGSVLNLGTKKHRDKFFDGIDNVDYPGC 181
           NEDPAKYFAIMEAVGS+DVS AIKMGVQFSLWGGSV+NLGTKKHRD+FFDGIDNVDYPGC
Sbjct: 123 NEDPAKYFAIMEAVGSVDVSLAIKMGVQFSLWGGSVINLGTKKHRDRFFDGIDNVDYPGC 182

Query: 182 FAMTELHHGSNVQGLQTTATFDPITDEFIINTPNDGAIKWWIGNAAVHGKFATVFAKLVL 241
           FAMTELHHGSNVQGLQTTATFDPITDEFIINTPNDGAIKWWIGNAAVHGKFATVFAKLVL
Sbjct: 183 FAMTELHHGSNVQGLQTTATFDPITDEFIINTPNDGAIKWWIGNAAVHGKFATVFAKLVL 242

Query: 242 PTHDSKKIADMGVHAFIVPIRDLKSHKTLPGIEIHDCGHKIGLNGVDNGALRFRSVRIPR 301
           PTHDS+K ADMGVHAFIVPIRDLKSHKTLPGIEIHDCGHK+GLNGVDNGALRFR VRIPR
Sbjct: 243 PTHDSRKTADMGVHAFIVPIRDLKSHKTLPGIEIHDCGHKVGLNGVDNGALRFRLVRIPR 302

Query: 302 DNLLDRFGEVSRDGKYKSSLPSISKRFAATLGELVGGRVGLAYSSASVLKVASTIAIRYS 361
           DNLLDRFGEVSRDGKYKSSLPSI+KRFAATLGELVGGRVGLAYSSASVLK+ASTIAIRYS
Sbjct: 303 DNLLDRFGEVSRDGKYKSSLPSINKRFAATLGELVGGRVGLAYSSASVLKIASTIAIRYS 362

Query: 362 LLRQQFGPLKQPEVSILDYQSQQHKLMPMLASTYAFHFATLQLVEKYAQMKKTHDEQL-A 421
           LLRQQFGP KQPEVSILDYQSQQHKLMPMLASTYAFHF+T+QLVEKYAQMKKTHDE+L  
Sbjct: 363 LLRQQFGPPKQPEVSILDYQSQQHKLMPMLASTYAFHFSTMQLVEKYAQMKKTHDEELVG 422

Query: 422 EIH--------------------------------------------------------- 481
           ++H                                                         
Sbjct: 423 DVHALSAGLKAYITSYTAKSLSTCREACGGHGYAVVNRFGTLRNDHDIFQTFEGDNTVLL 482

Query: 482 ------LLKQYKEKFEGGTLTVTWNYLRESMSTYLSQPNPVTARWEIPN----------- 541
                 LLKQYKEKF+GGTLTVTWNYLRESM+TYLSQPNPVTARWE  +           
Sbjct: 483 QQVAAYLLKQYKEKFQGGTLTVTWNYLRESMNTYLSQPNPVTARWESADHLRDPKFQLDA 542

Query: 542 -----VSITSSAAVRLRKHTKTLGSFGAWNRCLNHLLTLAESHIESVILAKFIEAVQSCP 601
                  +  S AVRLRKHTK LGSFGAWNRCLNHLLTLAESHIESVILA+FIEAVQ CP
Sbjct: 543 FQYRTSRLLQSVAVRLRKHTKNLGSFGAWNRCLNHLLTLAESHIESVILAQFIEAVQRCP 602

Query: 602 NASTQATLKLVCDLYALDRIWNDIGTYRNVDYVAPNKAKAIHKLTEYLCFQVRNIAKELV 607
           NA+TQATLKLVCDLYALDRIWNDIGTYRNVDYVAPNKAKAIHKLTEYLCFQVRNIA+ELV
Sbjct: 603 NANTQATLKLVCDLYALDRIWNDIGTYRNVDYVAPNKAKAIHKLTEYLCFQVRNIAQELV 662

BLAST of Sgr020616 vs. ExPASy TrEMBL
Match: A0A6J1C435 (Acyl-coenzyme A oxidase OS=Momordica charantia OX=3673 GN=LOC111008198 PE=3 SV=1)

HSP 1 Score: 1055.8 bits (2729), Expect = 1.1e-304
Identity = 542/686 (79.01%), Postives = 572/686 (83.38%), Query Frame = 0

Query: 1   MSSGEPNQMAEDESQAVSRRIQRLSFHLTPIPLPCGGSHQLEMLTCSAAKAKLEVDIASL 60
           MSSGE N+ AEDESQA+SRRI+RLS H+ PIP+P  GS QL+MLTC AAKAKLEVD ASL
Sbjct: 1   MSSGESNRTAEDESQAISRRIERLSLHMAPIPVPGDGS-QLQMLTCGAAKAKLEVDTASL 60

Query: 61  SLYMRGKHREIQERVFEYFNSRPELQTPVEISMSDHRELCMKQLIGLVREAGIRPYRYVN 120
           S YMRGKHREIQERVFEYFNSRPEL TPVEISM+DHRELCMKQLI LVREAGIRP R+VN
Sbjct: 61  SQYMRGKHREIQERVFEYFNSRPELHTPVEISMADHRELCMKQLIALVREAGIRPLRFVN 120

Query: 121 EDPAKYFAIMEAVGSIDVSFAIKMGVQFSLWGGSVLNLGTKKHRDKFFDGIDNVDYPGCF 180
           +DPAKYFAIMEAVGSIDVS AIKMGVQFSLWGGSVLNLGTKKHRDKFFDGIDN+DYPGCF
Sbjct: 121 DDPAKYFAIMEAVGSIDVSLAIKMGVQFSLWGGSVLNLGTKKHRDKFFDGIDNLDYPGCF 180

Query: 181 AMTELHHGSNVQGLQTTATFDPITDEFIINTPNDGAIKWWIGNAAVHGKFATVFAKLVLP 240
           AMTELHHGSNVQGLQTTATFDPITDEFIINTPNDGAIKWWIGNAAVHGKFATVFAKL+LP
Sbjct: 181 AMTELHHGSNVQGLQTTATFDPITDEFIINTPNDGAIKWWIGNAAVHGKFATVFAKLILP 240

Query: 241 THDSKKIADMGVHAFIVPIRDLKSHKTLPGIEIHDCGHKIGLNGVDNGALRFRSVRIPRD 300
           T+D+KK ADMGVHAFIVPIRDLKSHKTLPGIEIHDCGHK+GLNGVDNGALRF SVRIPRD
Sbjct: 241 TYDAKKTADMGVHAFIVPIRDLKSHKTLPGIEIHDCGHKVGLNGVDNGALRFSSVRIPRD 300

Query: 301 NLLDRFGEVSRDGKYKSSLPSISKRFAATLGELVGGRVGLAYSSASVLKVASTIAIRYSL 360
           NLLDRFGEVSRDGKYKSSLPSIS+RFAATLGELVGGRV LAYSS SVLK+ASTIAIRYSL
Sbjct: 301 NLLDRFGEVSRDGKYKSSLPSISRRFAATLGELVGGRVALAYSSVSVLKIASTIAIRYSL 360

Query: 361 LRQQFGPLKQPEVSILDYQSQQHKLMPMLASTYAFHFATLQLVEKYAQMKKTHDEQL-AE 420
           LRQQFGP KQPEVSILDYQSQQHKLMPMLAS YAFHFATLQLVEKYA MKKT+DE+L  +
Sbjct: 361 LRQQFGPPKQPEVSILDYQSQQHKLMPMLASNYAFHFATLQLVEKYALMKKTNDEELVGD 420

Query: 421 IH---------------------------------------------------------- 480
           +H                                                          
Sbjct: 421 VHALSAGLKAYVTSYTAKSLSTCREACGGHGYAAVNRFGSLRNDHDIFQTFEGDNTVLLQ 480

Query: 481 -----LLKQYKEKFEGGTLTVTWNYLRESMSTYLSQPNPVTARWEIPN------------ 540
                LLKQYKEKFEGGTL VTWNYLRESM+TYLSQPNPVTARWE  +            
Sbjct: 481 QVAAYLLKQYKEKFEGGTLAVTWNYLRESMNTYLSQPNPVTARWESADHLRDPKFQLDAF 540

Query: 541 ----VSITSSAAVRLRKHTKTLGSFGAWNRCLNHLLTLAESHIESVILAKFIEAVQSCPN 600
                 +  S AVRLRKHTKTLGSFGAWNRCLNHLLTLAESHIESVILA+FI+AVQ CPN
Sbjct: 541 QYRTSRLLQSVAVRLRKHTKTLGSFGAWNRCLNHLLTLAESHIESVILAQFIKAVQRCPN 600

Query: 601 ASTQATLKLVCDLYALDRIWNDIGTYRNVDYVAPNKAKAIHKLTEYLCFQVRNIAKELVD 607
           A+TQATLKL+CDLYALDRIWNDIGTYRNVDYVAPNKAKAIHKL EYL FQVRNIAKELVD
Sbjct: 601 ANTQATLKLLCDLYALDRIWNDIGTYRNVDYVAPNKAKAIHKLAEYLSFQVRNIAKELVD 660

BLAST of Sgr020616 vs. ExPASy TrEMBL
Match: A0A5D3CZ13 (Acyl-coenzyme A oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold21G005850 PE=3 SV=1)

HSP 1 Score: 1047.3 bits (2707), Expect = 3.9e-302
Identity = 532/687 (77.44%), Postives = 575/687 (83.70%), Query Frame = 0

Query: 2   SSGEPNQMAEDESQAVSRRIQRLSFHLTP-IPLPCGGSHQLEMLTCSA-AKAKLEVDIAS 61
           S+ E N+  EDES A SRRI+RLS HLTP IPLP  GS+++E++TC+  +K K++VD+ S
Sbjct: 3   SARESNRTVEDESHAASRRIERLSLHLTPMIPLPVDGSYRMEIVTCAGNSKVKMDVDMGS 62

Query: 62  LSLYMRGKHREIQERVFEYFNSRPELQTPVEISMSDHRELCMKQLIGLVREAGIRPYRYV 121
           LS++MRGKHREIQERVFE+FNSRPELQTPVEISM+DHRELCMKQLIG+VREAGI+P+R+V
Sbjct: 63  LSMFMRGKHREIQERVFEFFNSRPELQTPVEISMNDHRELCMKQLIGMVREAGIKPFRFV 122

Query: 122 NEDPAKYFAIMEAVGSIDVSFAIKMGVQFSLWGGSVLNLGTKKHRDKFFDGIDNVDYPGC 181
           NEDP KYFAIMEAVGS+DVS AIKMGVQFSLWGGSVLNLGTKKHRDKFFDGIDNVDYPGC
Sbjct: 123 NEDPGKYFAIMEAVGSVDVSLAIKMGVQFSLWGGSVLNLGTKKHRDKFFDGIDNVDYPGC 182

Query: 182 FAMTELHHGSNVQGLQTTATFDPITDEFIINTPNDGAIKWWIGNAAVHGKFATVFAKLVL 241
           FAMTELHHGSNVQGLQTTATFD ITDEFIINTPNDGAIKWWIGNAAVHGKFATVFAKLVL
Sbjct: 183 FAMTELHHGSNVQGLQTTATFDLITDEFIINTPNDGAIKWWIGNAAVHGKFATVFAKLVL 242

Query: 242 PTHDSKKIADMGVHAFIVPIRDLKSHKTLPGIEIHDCGHKIGLNGVDNGALRFRSVRIPR 301
           PTH SKK ADMGVHAFIVPIRDLK+HKTLPGIEIHDCGHK+GLNGVDNGALRFRSVRIPR
Sbjct: 243 PTHGSKKTADMGVHAFIVPIRDLKTHKTLPGIEIHDCGHKVGLNGVDNGALRFRSVRIPR 302

Query: 302 DNLLDRFGEVSRDGKYKSSLPSISKRFAATLGELVGGRVGLAYSSASVLKVASTIAIRYS 361
           DNLLDRFG+VSRDGKYKSSLPSI+KRFAATLGELVGGRVGLAYSSASVLK+ASTIAIRYS
Sbjct: 303 DNLLDRFGQVSRDGKYKSSLPSINKRFAATLGELVGGRVGLAYSSASVLKIASTIAIRYS 362

Query: 362 LLRQQFGPLKQPEVSILDYQSQQHKLMPMLASTYAFHFATLQLVEKYAQMKKTHDEQL-A 421
           LLRQQFGP KQPEVSILDYQSQQHKLMPMLASTYAF FAT+QLVEKYAQMKKTHDE+L  
Sbjct: 363 LLRQQFGPPKQPEVSILDYQSQQHKLMPMLASTYAFQFATIQLVEKYAQMKKTHDEELVG 422

Query: 422 EIH--------------------------------------------------------- 481
           ++H                                                         
Sbjct: 423 DVHALSAGLKAYITSYTAKSLSTCREACGGHGYAAVNRFGTLRNDHDIFQTFEGDNTVLL 482

Query: 482 ------LLKQYKEKFEGGTLTVTWNYLRESMSTYLSQPNPVTARWEIPN----------- 541
                 LLKQYKEKF+GGTL VTWNYLRESM+TYLSQPNPVTARWE  +           
Sbjct: 483 QQVAAYLLKQYKEKFQGGTLAVTWNYLRESMNTYLSQPNPVTARWESTDHLRDPKFQLDA 542

Query: 542 -----VSITSSAAVRLRKHTKTLGSFGAWNRCLNHLLTLAESHIESVILAKFIEAVQSCP 601
                  +  S AVRLRKHTKTLG FGAWNRCLNHLLTLAESHIESVILA+FIEA+Q CP
Sbjct: 543 FQYRTSRLLQSVAVRLRKHTKTLGGFGAWNRCLNHLLTLAESHIESVILAQFIEAIQRCP 602

Query: 602 NASTQATLKLVCDLYALDRIWNDIGTYRNVDYVAPNKAKAIHKLTEYLCFQVRNIAKELV 607
           NA+TQATLKLVCDLYALDRIWNDIGTYRNVDYVAPNKAKAIHKLTEYLCFQVRNIA+ELV
Sbjct: 603 NANTQATLKLVCDLYALDRIWNDIGTYRNVDYVAPNKAKAIHKLTEYLCFQVRNIAQELV 662

BLAST of Sgr020616 vs. ExPASy TrEMBL
Match: A0A1S3BX82 (Acyl-coenzyme A oxidase OS=Cucumis melo OX=3656 GN=LOC103494416 PE=3 SV=1)

HSP 1 Score: 1047.3 bits (2707), Expect = 3.9e-302
Identity = 532/687 (77.44%), Postives = 575/687 (83.70%), Query Frame = 0

Query: 2   SSGEPNQMAEDESQAVSRRIQRLSFHLTP-IPLPCGGSHQLEMLTCSA-AKAKLEVDIAS 61
           S+ E N+  EDES A SRRI+RLS HLTP IPLP  GS+++E++TC+  +K K++VD+ S
Sbjct: 3   SARESNRTVEDESHAASRRIERLSLHLTPMIPLPVDGSYRMEIVTCAGNSKVKMDVDMGS 62

Query: 62  LSLYMRGKHREIQERVFEYFNSRPELQTPVEISMSDHRELCMKQLIGLVREAGIRPYRYV 121
           LS++MRGKHREIQERVFE+FNSRPELQTPVEISM+DHRELCMKQLIG+VREAGI+P+R+V
Sbjct: 63  LSMFMRGKHREIQERVFEFFNSRPELQTPVEISMNDHRELCMKQLIGMVREAGIKPFRFV 122

Query: 122 NEDPAKYFAIMEAVGSIDVSFAIKMGVQFSLWGGSVLNLGTKKHRDKFFDGIDNVDYPGC 181
           NEDP KYFAIMEAVGS+DVS AIKMGVQFSLWGGSVLNLGTKKHRDKFFDGIDNVDYPGC
Sbjct: 123 NEDPGKYFAIMEAVGSVDVSLAIKMGVQFSLWGGSVLNLGTKKHRDKFFDGIDNVDYPGC 182

Query: 182 FAMTELHHGSNVQGLQTTATFDPITDEFIINTPNDGAIKWWIGNAAVHGKFATVFAKLVL 241
           FAMTELHHGSNVQGLQTTATFD ITDEFIINTPNDGAIKWWIGNAAVHGKFATVFAKLVL
Sbjct: 183 FAMTELHHGSNVQGLQTTATFDLITDEFIINTPNDGAIKWWIGNAAVHGKFATVFAKLVL 242

Query: 242 PTHDSKKIADMGVHAFIVPIRDLKSHKTLPGIEIHDCGHKIGLNGVDNGALRFRSVRIPR 301
           PTH SKK ADMGVHAFIVPIRDLK+HKTLPGIEIHDCGHK+GLNGVDNGALRFRSVRIPR
Sbjct: 243 PTHGSKKTADMGVHAFIVPIRDLKTHKTLPGIEIHDCGHKVGLNGVDNGALRFRSVRIPR 302

Query: 302 DNLLDRFGEVSRDGKYKSSLPSISKRFAATLGELVGGRVGLAYSSASVLKVASTIAIRYS 361
           DNLLDRFG+VSRDGKYKSSLPSI+KRFAATLGELVGGRVGLAYSSASVLK+ASTIAIRYS
Sbjct: 303 DNLLDRFGQVSRDGKYKSSLPSINKRFAATLGELVGGRVGLAYSSASVLKIASTIAIRYS 362

Query: 362 LLRQQFGPLKQPEVSILDYQSQQHKLMPMLASTYAFHFATLQLVEKYAQMKKTHDEQL-A 421
           LLRQQFGP KQPEVSILDYQSQQHKLMPMLASTYAF FAT+QLVEKYAQMKKTHDE+L  
Sbjct: 363 LLRQQFGPPKQPEVSILDYQSQQHKLMPMLASTYAFQFATIQLVEKYAQMKKTHDEELVG 422

Query: 422 EIH--------------------------------------------------------- 481
           ++H                                                         
Sbjct: 423 DVHALSAGLKAYITSYTAKSLSTCREACGGHGYAAVNRFGTLRNDHDIFQTFEGDNTVLL 482

Query: 482 ------LLKQYKEKFEGGTLTVTWNYLRESMSTYLSQPNPVTARWEIPN----------- 541
                 LLKQYKEKF+GGTL VTWNYLRESM+TYLSQPNPVTARWE  +           
Sbjct: 483 QQVAAYLLKQYKEKFQGGTLAVTWNYLRESMNTYLSQPNPVTARWESTDHLRDPKFQLDA 542

Query: 542 -----VSITSSAAVRLRKHTKTLGSFGAWNRCLNHLLTLAESHIESVILAKFIEAVQSCP 601
                  +  S AVRLRKHTKTLG FGAWNRCLNHLLTLAESHIESVILA+FIEA+Q CP
Sbjct: 543 FQYRTSRLLQSVAVRLRKHTKTLGGFGAWNRCLNHLLTLAESHIESVILAQFIEAIQRCP 602

Query: 602 NASTQATLKLVCDLYALDRIWNDIGTYRNVDYVAPNKAKAIHKLTEYLCFQVRNIAKELV 607
           NA+TQATLKLVCDLYALDRIWNDIGTYRNVDYVAPNKAKAIHKLTEYLCFQVRNIA+ELV
Sbjct: 603 NANTQATLKLVCDLYALDRIWNDIGTYRNVDYVAPNKAKAIHKLTEYLCFQVRNIAQELV 662

BLAST of Sgr020616 vs. TAIR 10
Match: AT5G65110.1 (acyl-CoA oxidase 2 )

HSP 1 Score: 953.7 bits (2464), Expect = 1.1e-277
Identity = 477/686 (69.53%), Postives = 545/686 (79.45%), Query Frame = 0

Query: 2   SSGEPNQMAEDESQAV--SRRIQRLSFHLTPIPLPCGGSHQLEMLTCSAAKAKLEVDIAS 61
           S  E N M E+ES  +  +RRIQRLS HL+P   P      ++  TCSA   KL+V+  +
Sbjct: 3   SRREKNPMTEEESDGLIAARRIQRLSLHLSPSLTPSPSLPLVQTETCSARSKKLDVNGEA 62

Query: 62  LSLYMRGKHREIQERVFEYFNSRPELQTPVEISMSDHRELCMKQLIGLVREAGIRPYRYV 121
           LSLYMRGKH +IQE++F++FNSRP+LQTP+EIS  DHRELCM QLIGLVREAG+RP+RYV
Sbjct: 63  LSLYMRGKHIDIQEKIFDFFNSRPDLQTPIEISKDDHRELCMNQLIGLVREAGVRPFRYV 122

Query: 122 NEDPAKYFAIMEAVGSIDVSFAIKMGVQFSLWGGSVLNLGTKKHRDKFFDGIDNVDYPGC 181
            +DP KYFAIMEAVGS+D+S  IKMGVQ+SLWGGSV+NLGTKKHRDK+FDGIDN+DY GC
Sbjct: 123 ADDPEKYFAIMEAVGSVDMSLGIKMGVQYSLWGGSVINLGTKKHRDKYFDGIDNLDYTGC 182

Query: 182 FAMTELHHGSNVQGLQTTATFDPITDEFIINTPNDGAIKWWIGNAAVHGKFATVFAKLVL 241
           FAMTELHHGSNVQGLQTTATFDP+ DEF+I+TPNDGAIKWWIGNAAVHGKFATVFA+L+L
Sbjct: 183 FAMTELHHGSNVQGLQTTATFDPLKDEFVIDTPNDGAIKWWIGNAAVHGKFATVFARLIL 242

Query: 242 PTHDSKKIADMGVHAFIVPIRDLKSHKTLPGIEIHDCGHKIGLNGVDNGALRFRSVRIPR 301
           PTHDSK ++DMGVHAFIVPIRD+K+H+TLPG+EI DCGHK+GLNGVDNGALRFRSVRIPR
Sbjct: 243 PTHDSKGVSDMGVHAFIVPIRDMKTHQTLPGVEIQDCGHKVGLNGVDNGALRFRSVRIPR 302

Query: 302 DNLLDRFGEVSRDGKYKSSLPSISKRFAATLGELVGGRVGLAYSSASVLKVASTIAIRYS 361
           DNLL+RFG+VSRDG Y SSLP+I+KRF ATLGELVGGRVGLAY+S  VLK+++TIAIRYS
Sbjct: 303 DNLLNRFGDVSRDGTYTSSLPTINKRFGATLGELVGGRVGLAYASVGVLKISATIAIRYS 362

Query: 362 LLRQQFGPLKQPEVSILDYQSQQHKLMPMLASTYAFHFATLQLVEKYAQMKKTHDEQL-A 421
           LLRQQFGP KQPEVSILDYQSQQHKLMPMLASTYA+HFAT+ LVEKY++MKKTHDEQL A
Sbjct: 363 LLRQQFGPPKQPEVSILDYQSQQHKLMPMLASTYAYHFATVYLVEKYSEMKKTHDEQLVA 422

Query: 422 EIH--------------------------------------------------------- 481
           ++H                                                         
Sbjct: 423 DVHALSAGLKSYVTSYTAKALSVCREACGGHGYAAVNRFGSLRNDHDIFQTFEGDNTVLL 482

Query: 482 ------LLKQYKEKFEGGTLTVTWNYLRESMSTYLSQPNPVTARWE-------------- 541
                 LLK+YKEKF+GGTLTVTW+YLRESM+TYLSQPNPVTARWE              
Sbjct: 483 QQVAADLLKRYKEKFQGGTLTVTWSYLRESMNTYLSQPNPVTARWEGEDHLRDPKFQLDA 542

Query: 542 --IPNVSITSSAAVRLRKHTKTLGSFGAWNRCLNHLLTLAESHIESVILAKFIEAVQSCP 601
                  +  + A RL+KH+KTLG FGAWNRCLNHLLTLAESHIE+VILAKFIEAV++CP
Sbjct: 543 FRYRTSRLLQNVAARLQKHSKTLGGFGAWNRCLNHLLTLAESHIETVILAKFIEAVKNCP 602

Query: 602 NASTQATLKLVCDLYALDRIWNDIGTYRNVDYVAPNKAKAIHKLTEYLCFQVRNIAKELV 606
           + S +A LKL CDLYALDRIW DIGTYRNVDYVAPNKAKAIHKLTEYL FQVRN+AKELV
Sbjct: 603 DPSAKAALKLACDLYALDRIWKDIGTYRNVDYVAPNKAKAIHKLTEYLSFQVRNVAKELV 662

BLAST of Sgr020616 vs. TAIR 10
Match: AT5G65110.2 (acyl-CoA oxidase 2 )

HSP 1 Score: 869.0 bits (2244), Expect = 3.7e-252
Identity = 435/639 (68.08%), Postives = 499/639 (78.09%), Query Frame = 0

Query: 2   SSGEPNQMAEDESQAV--SRRIQRLSFHLTPIPLPCGGSHQLEMLTCSAAKAKLEVDIAS 61
           S  E N M E+ES  +  +RRIQRLS HL+P   P      ++  TCSA   KL+V+  +
Sbjct: 3   SRREKNPMTEEESDGLIAARRIQRLSLHLSPSLTPSPSLPLVQTETCSARSKKLDVNGEA 62

Query: 62  LSLYMRGKHREIQERVFEYFNSRPELQTPVEISMSDHRELCMKQLIGLVREAGIRPYRYV 121
           LSLYMRGKH +IQE++F++FNSRP+LQTP+EIS  DHRELCM QLIGLVREAG+RP+RYV
Sbjct: 63  LSLYMRGKHIDIQEKIFDFFNSRPDLQTPIEISKDDHRELCMNQLIGLVREAGVRPFRYV 122

Query: 122 NEDPAKYFAIMEAVGSIDVSFAIKMGVQFSLWGGSVLNLGTKKHRDKFFDGIDNVDYPGC 181
            +DP KYFAIMEAVGS+D+S  IKMGVQ+SLWGGSV+NLGTKKHRDK+FDGIDN+DY GC
Sbjct: 123 ADDPEKYFAIMEAVGSVDMSLGIKMGVQYSLWGGSVINLGTKKHRDKYFDGIDNLDYTGC 182

Query: 182 FAMTELHHGSNVQGLQTTATFDPITDEFIINTPNDGAIKWWIGNAAVHGKFATVFAKLVL 241
           FAMTELHHGSNVQGLQTTATFDP+ DEF+I+TPNDGAIKWWIGNAAVHGKFATVFA+L+L
Sbjct: 183 FAMTELHHGSNVQGLQTTATFDPLKDEFVIDTPNDGAIKWWIGNAAVHGKFATVFARLIL 242

Query: 242 PTHDSKKIADMGVHAFIVPIRDLKSHKTLPGIEIHDCGHKIGLNGVDNGALRFRSVRIPR 301
           PTHDSK ++DMGVHAFIVPIRD+K+H+TLPG+EI DCGHK+GLNGVDNGALRFRSVRIPR
Sbjct: 243 PTHDSKGVSDMGVHAFIVPIRDMKTHQTLPGVEIQDCGHKVGLNGVDNGALRFRSVRIPR 302

Query: 302 DNLLDRFGEVSRDGKYKSSLPSISKRFAATLGELVGGRVGLAYSSASVLKVASTIAIRYS 361
           DNLL+RFG+VSRDG Y SSLP+I+KRF ATLGELVGGRVGLAY+S  VLK+++TIAIRYS
Sbjct: 303 DNLLNRFGDVSRDGTYTSSLPTINKRFGATLGELVGGRVGLAYASVGVLKISATIAIRYS 362

Query: 362 LLRQQFGPLKQPEVSILDYQSQQHKLMPMLASTYAFHFATLQLVEKYAQMKKTHDEQL-A 421
           LLRQQFGP KQPEVSILDYQSQQHKLMPMLASTYA+HFAT+ LVEKY++MKKTHDEQL A
Sbjct: 363 LLRQQFGPPKQPEVSILDYQSQQHKLMPMLASTYAYHFATVYLVEKYSEMKKTHDEQLVA 422

Query: 422 EIH--------------------------------------------------------- 481
           ++H                                                         
Sbjct: 423 DVHALSAGLKSYVTSYTAKALSVCREACGGHGYAAVNRFGSLRNDHDIFQTFEGDNTVLL 482

Query: 482 ------LLKQYKEKFEGGTLTVTWNYLRESMSTYLSQPNPVTARWE-------------- 541
                 LLK+YKEKF+GGTLTVTW+YLRESM+TYLSQPNPVTARWE              
Sbjct: 483 QQVAADLLKRYKEKFQGGTLTVTWSYLRESMNTYLSQPNPVTARWEGEDHLRDPKFQLDA 542

Query: 542 --IPNVSITSSAAVRLRKHTKTLGSFGAWNRCLNHLLTLAESHIESVILAKFIEAVQSCP 559
                  +  + A RL+KH+KTLG FGAWNRCLNHLLTLAESHIE+VILAKFIEAV++CP
Sbjct: 543 FRYRTSRLLQNVAARLQKHSKTLGGFGAWNRCLNHLLTLAESHIETVILAKFIEAVKNCP 602

BLAST of Sgr020616 vs. TAIR 10
Match: AT1G06290.1 (acyl-CoA oxidase 3 )

HSP 1 Score: 261.2 bits (666), Expect = 3.5e-69
Identity = 151/352 (42.90%), Postives = 207/352 (58.81%), Query Frame = 0

Query: 92  SMSDHRELCMKQLIGLVREAGIRPYRYVN--EDPAKYFAIMEAVGSIDVSFAIKMGVQFS 151
           +M   RE+ MK++  L+     + +      E   +  A++E  G  D S +IK+GV F 
Sbjct: 96  TMEQQREITMKRIWYLLENGVFKGWLTETGPEAELRKLALLEVCGIYDHSVSIKVGVHFF 155

Query: 152 LWGGSVLNLGTKKHRDKFFDGIDNVDYPGCFAMTELHHGSNVQGLQTTATFDPITDEFII 211
           LWG +V   GTK+H +K+    ++    GCFAMTEL HGSNV+G++T  T+DP T+EF+I
Sbjct: 156 LWGNAVKFFGTKRHHEKWLKNTEDYVVKGCFAMTELGHGSNVRGIETVTTYDPKTEEFVI 215

Query: 212 NTPNDGAIKWWIGNAAVHGKFATVFAKLVLPTHDSKKIADMGVHAFIVPIRDLKSHKTLP 271
           NTP + A K+WIG AA H     VF++L +         + GVHAFI  IRD +     P
Sbjct: 216 NTPCESAQKYWIGGAANHATHTIVFSQLHI------NGTNQGVHAFIAQIRD-QDGSICP 275

Query: 272 GIEIHDCGHKIGLNGVDNGALRFRSVRIPRDNLLDRFGEVSRDGKYKSSLPSISKRFAAT 331
            I I DCGHKIGLNGVDNG + F ++RIPR+NLL+   +VS DGKY SS+    +RF A 
Sbjct: 276 NIRIADCGHKIGLNGVDNGRIWFDNLRIPRENLLNAVADVSSDGKYVSSIKDPDQRFGAF 335

Query: 332 LGELVGGRVGLAYSSASVLKVASTIAIRYSLLRQQFG-PLKQPEVSILDYQSQQHKLMPM 391
           +  L  GRV +A S+    KV  +IAIRYSL R+ F      PEV +LDY S Q +L+P+
Sbjct: 336 MAPLTSGRVTIASSAIYSAKVGLSIAIRYSLSRRAFSVTANGPEVLLLDYPSHQRRLLPL 395

Query: 392 LASTYAFHFATLQLVEKYAQMKKTHDEQLAEIHLLKQYKEKFEGGTLTVTWN 441
           LA TYA  FA  +L  K   +K+T +   A IH++        G    +TW+
Sbjct: 396 LAKTYAMSFAANEL--KMIYVKRTPETNKA-IHVVS------SGFKAVLTWH 431

BLAST of Sgr020616 vs. TAIR 10
Match: AT1G06310.1 (acyl-CoA oxidase 6 )

HSP 1 Score: 260.4 bits (664), Expect = 5.9e-69
Identity = 148/355 (41.69%), Postives = 205/355 (57.75%), Query Frame = 0

Query: 92  SMSDHRELCMKQLIGLVREAGIRPYRYVN--EDPAKYFAIMEAVGSIDVSFAIKMGVQFS 151
           +M   R++ MK++  L+ +   + +      E   K FA+ E  G  D S + K+GV F 
Sbjct: 96  TMEQQRQISMKRIFYLLEKGVFQGWLTETGPEAELKKFALYEVCGIYDYSLSAKLGVHFL 155

Query: 152 LWGGSVLNLGTKKHRDKFFDGIDNVDYPGCFAMTELHHGSNVQGLQTTATFDPITDEFII 211
           LWG +V   GTK+H +K+    ++    GCFAMTEL HG+NV+G++T  T+DP T+EF+I
Sbjct: 156 LWGNAVKFFGTKRHHEKWLKDTEDYVVKGCFAMTELGHGTNVRGIETVTTYDPTTEEFVI 215

Query: 212 NTPNDGAIKWWIGNAAVHGKFATVFAKLVLPTHDSKKIADMGVHAFIVPIRDLKSHKTLP 271
           NTP + A K+WIG AA H   A V ++L      S    + G+H FI  IRD     T P
Sbjct: 216 NTPCESAQKYWIGEAANHANHAIVISQL------SMNGTNQGIHVFIAQIRD-HDGNTCP 275

Query: 272 GIEIHDCGHKIGLNGVDNGALRFRSVRIPRDNLLDRFGEVSRDGKYKSSLPSISKRFAAT 331
            + I DCGHKIGLNGVDNG + F ++RIPR+NLL+   +V  DGKY SS+    +RF A 
Sbjct: 276 NVRIADCGHKIGLNGVDNGRIWFDNLRIPRENLLNSVADVLADGKYVSSIKDPDQRFGAF 335

Query: 332 LGELVGGRVGLAYSSASVLKVASTIAIRYSLLRQQFG-PLKQPEVSILDYQSQQHKLMPM 391
           L  L  GRV +A S+    K+   +AIRYSL R+ F      PEV +LDY S Q +L+P+
Sbjct: 336 LAPLTSGRVTIASSAIYSAKLGLAVAIRYSLSRRAFSVAANGPEVLLLDYPSHQRRLLPL 395

Query: 392 LASTYAFHFATLQLVEKYAQMKKTHDEQLAEIHLLKQYKEKFEGGTLTVTWNYLR 444
           LA TYA  FA   L  K   +K+T +   A IH++        G    +TW+ +R
Sbjct: 396 LAKTYAMSFAVNDL--KMIYVKRTPETNKA-IHVVS------SGFKAVLTWHNMR 434

BLAST of Sgr020616 vs. TAIR 10
Match: AT4G16760.1 (acyl-CoA oxidase 1 )

HSP 1 Score: 187.2 bits (474), Expect = 6.4e-47
Identity = 106/254 (41.73%), Postives = 148/254 (58.27%), Query Frame = 0

Query: 151 WG---GSVLNLGTKKHRDKFFDGIDNVDYPGCFAMTELHHGSNVQGLQTTATFDPITDEF 210
           WG    ++   GT++ + K+    + +   GC+A TEL HGSNVQGL+TTATFDP TDEF
Sbjct: 103 WGMFVPAIKGQGTEEQQKKWLSLANKMQIIGCYAQTELGHGSNVQGLETTATFDPKTDEF 162

Query: 211 IINTPNDGAIKWWIGNAAVHGKFATVFAKLVLPTHDSKKIADMGVHAFIVPIRDLKSHKT 270
           +I+TP   A KWW G        A V+A+L+          D G+H FIV +R L+ H  
Sbjct: 163 VIHTPTQTASKWWPGGLGKVSTHAVVYARLI------TNGKDYGIHGFIVQLRSLEDHSP 222

Query: 271 LPGIEIHDCGHKIG---LNGVDNGALRFRSVRIPRDNLLDRFGEVSRDGKYKSSLPSISK 330
           LP I + D G K+G    N +DNG L F  VRIPRD +L R  +V+R+G+Y  S   + K
Sbjct: 223 LPNITVGDIGTKMGNGAYNSMDNGFLMFDHVRIPRDQMLMRLSKVTREGEYVPS--DVPK 282

Query: 331 RFAATLGELVGGRVGLAYSSASVLKVASTIAIRYSLLRQQFGPLKQP-EVSILDYQSQQH 390
           +     G +V  R  +   +++ L  A  IA RYS +R+QFG      E  ++DY++QQ+
Sbjct: 283 QL--VYGTMVYVRQTIVADASNALSRAVCIATRYSAVRRQFGAHNGGIETQVIDYKTQQN 342

Query: 391 KLMPMLASTYAFHF 398
           +L P+LAS YAF F
Sbjct: 343 RLFPLLASAYAFRF 346