Sgr019381 (gene) Monk fruit (Qingpiguo) v1

BLAST of Sgr019381 vs. NCBI nr
Match: XP_022155827.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Momordica charantia])

HSP 1 Score: 399.8 bits (1026), Expect = 1.7e-107
Identity = 186/252 (73.81%), Postives = 207/252 (82.14%), Query Frame = 0

Query: 4   MEASKQEPKKMVK-PPKPFGRSKWEMLDKNVPFTHLLLLHVPCIFAPFHFNWGAFWVAIT 63
           MEASK+EPK+MVK PP PF R    ML +   F   +LLH+ CI APFHFNW AFWVA  
Sbjct: 1   MEASKEEPKEMVKPPPSPFER---RMLRRRTVFAPFILLHLLCILAPFHFNWKAFWVAFG 60

Query: 64  LYNLTAMAISISYHRNLAHRSFKLPKWLEYLLAYCVAHAFQGDPISWVSIHRCHHRYVDT 123
           L   T + IS+SYHRNLAHRSFKLPKWLEYLL YC AHAFQGDPI WVS HRCHHRYVDT
Sbjct: 61  LATTTGLMISVSYHRNLAHRSFKLPKWLEYLLVYCAAHAFQGDPIDWVSTHRCHHRYVDT 120

Query: 124 EKDPHSPIQGFWFSHITWLFDLYTLNEKVCPKYYSDLQKRERNIYMLFFKYGNSDNVTDL 183
           E+DPHSP QGFWFSHITWLFD YTL +KVCP Y +   K E+NI+MLF K+GN DNV DL
Sbjct: 121 ERDPHSPTQGFWFSHITWLFDSYTLTKKVCPNYSNGFGKIEKNIFMLFLKHGNPDNVPDL 180

Query: 184 EKQTFYRFMQQTYILHPIALALLLYAIGGVPFIIWGVCVRIAVSLQITFLVNSVCHMWGK 243
           EKQ FYRFMQ+TY LHPIALA+LLYA+GGVPFIIWG+CVRI VSL ITF+VNSVCH+WG+
Sbjct: 181 EKQVFYRFMQKTYFLHPIALAVLLYAVGGVPFIIWGMCVRITVSLHITFMVNSVCHIWGE 240

Query: 244 QQWNTSDLSKNN 255
           QQW+T DLSKNN
Sbjct: 241 QQWDTGDLSKNN 249

BLAST of Sgr019381 vs. NCBI nr
Match: XP_022155832.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Momordica charantia])

HSP 1 Score: 394.4 bits (1012), Expect = 7.3e-106
Identity = 179/252 (71.03%), Postives = 210/252 (83.33%), Query Frame = 0

Query: 4   MEASKQEPKKMVK-PPKPFGRSKWEMLDKNVPFTHLLLLHVPCIFAPFHFNWGAFWVAIT 63
           MEASKQEPK+MVK P +PF R  W  LDKN+  + L LLH+ CI APFHFNWGAFW+ + 
Sbjct: 1   MEASKQEPKEMVKLPLRPFDRKTWTKLDKNIA-SSLFLLHLLCILAPFHFNWGAFWIFLV 60

Query: 64  LYNLTAMAISISYHRNLAHRSFKLPKWLEYLLAYCVAHAFQGDPISWVSIHRCHHRYVDT 123
           L NLT +AIS+SYH+NLAHRSFKLPKWLEYLLAY  AHAFQGDPI WVS HR HH+YVDT
Sbjct: 61  LTNLTGIAISVSYHKNLAHRSFKLPKWLEYLLAYYAAHAFQGDPIDWVSTHRSHHQYVDT 120

Query: 124 EKDPHSPIQGFWFSHITWLFDLYTLNEKVCPKYYSDLQKRERNIYMLFFKYGNSDNVTDL 183
           E+DPHSP  GFWFSHI WLFD YTL ++VCP Y++D Q+ ERNI+M + K+GN  NV DL
Sbjct: 121 ERDPHSPTNGFWFSHIIWLFDSYTLTKRVCPNYFNDFQEIERNIFMTYLKHGNPSNVHDL 180

Query: 184 EKQTFYRFMQQTYILHPIALALLLYAIGGVPFIIWGVCVRIAVSLQITFLVNSVCHMWGK 243
           EKQ FYRFMQ+TY+LH  ALA+LLY +GGVP+IIWG+C+RI V+L +TF+VNSVCH+WGK
Sbjct: 181 EKQAFYRFMQKTYLLHIFALAILLYFVGGVPYIIWGMCMRIVVTLHLTFMVNSVCHIWGK 240

Query: 244 QQWNTSDLSKNN 255
           QQWNT DLSKNN
Sbjct: 241 QQWNTRDLSKNN 251

BLAST of Sgr019381 vs. NCBI nr
Match: XP_022155833.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Momordica charantia])

HSP 1 Score: 384.0 bits (985), Expect = 9.9e-103
Identity = 178/252 (70.63%), Postives = 203/252 (80.56%), Query Frame = 0

Query: 4   MEASKQEPKKMVK-PPKPFGRSKWEMLDKNVPFTHLLLLHVPCIFAPFHFNWGAFWVAIT 63
           MEASKQEPK MV+ P +PF R KW  LDKNV  +   +LH+ CI APF+FNWGAFWV + 
Sbjct: 1   MEASKQEPKDMVRLPLRPFERKKWTKLDKNVA-SSFFILHLLCILAPFNFNWGAFWVFLV 60

Query: 64  LYNLTAMAISISYHRNLAHRSFKLPKWLEYLLAYCVAHAFQGDPISWVSIHRCHHRYVDT 123
           + +LT MAIS+SYHRNLAHRSFKLPKWLEYLLAYC AHAFQGDPI WVS HR HHRYVDT
Sbjct: 61  VGSLTGMAISVSYHRNLAHRSFKLPKWLEYLLAYCAAHAFQGDPIDWVSTHRSHHRYVDT 120

Query: 124 EKDPHSPIQGFWFSHITWLFDLYTLNEKVCPKYYSDLQKRERNIYMLFFKYGNSDNVTDL 183
           E+DPHSPI GFWFS ITWLFD Y+L  K CP Y+ D +K ERNI+    KY N  NV DL
Sbjct: 121 ERDPHSPINGFWFSQITWLFDSYSLTTKNCPNYFGDFRKIERNIFTGSLKYANPSNVPDL 180

Query: 184 EKQTFYRFMQQTYILHPIALALLLYAIGGVPFIIWGVCVRIAVSLQITFLVNSVCHMWGK 243
           EKQ FYRFMQ+TY+LH +AL +LLY IGGVP+IIWG+C+RI VS  +T +VNSVCHMWGK
Sbjct: 181 EKQAFYRFMQKTYLLHLLALTILLYVIGGVPYIIWGMCMRIVVSFHLTSMVNSVCHMWGK 240

Query: 244 QQWNTSDLSKNN 255
           +QWNT DLSKNN
Sbjct: 241 KQWNTDDLSKNN 251

BLAST of Sgr019381 vs. NCBI nr
Match: XP_038893002.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 376.7 bits (966), Expect = 1.6e-100
Identity = 164/231 (71.00%), Postives = 192/231 (83.12%), Query Frame = 0

Query: 24  SKWEMLDKNVPFTHLLLLHVPCIFAPFHFNWGAFWVAITLYNLTAMAISISYHRNLAHRS 83
           +KWEMLD NVP T++L + + C FAPFHFNW + W A  LY +T + +SISYHRNLAHRS
Sbjct: 2   TKWEMLDINVPSTYILFVQMLCFFAPFHFNWASLWTAFALYVVTGLGVSISYHRNLAHRS 61

Query: 84  FKLPKWLEYLLAYCVAHAFQGDPISWVSIHRCHHRYVDTEKDPHSPIQGFWFSHITWLFD 143
           FKL KWLEYLLAYC  HAFQGDPISWVSIHRCHHRYVDTE+DPHSPI GF FSHI WL D
Sbjct: 62  FKLSKWLEYLLAYCAVHAFQGDPISWVSIHRCHHRYVDTERDPHSPIHGFLFSHIAWLLD 121

Query: 144 LYTLNEKVCPKYYSDLQKRERNIYMLFFKYGNSDNVTDLEKQTFYRFMQQTYILHPIALA 203
           + TLN+KVCPKY+SD + +ER +Y LFFK GN DNV DLEKQTFYRF+Q++YI HP+AL 
Sbjct: 122 IPTLNKKVCPKYFSDTRNQERKMYKLFFKQGNPDNVPDLEKQTFYRFIQKSYIFHPMALG 181

Query: 204 LLLYAIGGVPFIIWGVCVRIAVSLQITFLVNSVCHMWGKQQWNTSDLSKNN 255
           +L YAIGGVP  IWG+CVR+ V+   TF+VNS+CH+WGKQQWNT DLS+NN
Sbjct: 182 VLFYAIGGVPLFIWGMCVRLTVTSHATFMVNSICHIWGKQQWNTGDLSRNN 232

BLAST of Sgr019381 vs. NCBI nr
Match: XP_022153644.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Momordica charantia])

HSP 1 Score: 375.6 bits (963), Expect = 3.5e-100
Identity = 165/252 (65.48%), Postives = 204/252 (80.95%), Query Frame = 0

Query: 4   MEASKQEPKKMVKPPKPFGRSKWEMLDKNVPFTHLLLLHVPCIFAPFHFNWGAFWVAITL 63
           MEASKQEPK+M+KPP+P+ R KW  LDK++  + L L+H+ CI APF FNW AFWVA  L
Sbjct: 1   MEASKQEPKEMMKPPRPYERRKWTKLDKDIACS-LFLVHLLCILAPFQFNWSAFWVAFVL 60

Query: 64  YNLTAM-AISISYHRNLAHRSFKLPKWLEYLLAYCVAHAFQGDPISWVSIHRCHHRYVDT 123
           Y +T +  +++SYHRNLAH+SFKLPKWLEYL AYC   A QGDP+ WVS HRCHH++VDT
Sbjct: 61  YIVTGLFGMTLSYHRNLAHKSFKLPKWLEYLFAYCGVQALQGDPMDWVSTHRCHHQFVDT 120

Query: 124 EKDPHSPIQGFWFSHITWLFDLYTLNEKVCPKYYSDLQKRERNIYMLFFKYGNSDNVTDL 183
           E+DPHSPI+GFWFSHI WLFD Y L +KVCPKY+S+ QK ERNI++LF K+G  +NV DL
Sbjct: 121 ERDPHSPIRGFWFSHILWLFDSYNLTKKVCPKYFSNFQKLERNIFVLFSKHGKPENVADL 180

Query: 184 EKQTFYRFMQQTYILHPIALALLLYAIGGVPFIIWGVCVRIAVSLQITFLVNSVCHMWGK 243
           EKQ FYRF+ QTY+LHPI LA++LY +GG+PF++WG+ VRI V L  T +VNSVCH+WGK
Sbjct: 181 EKQAFYRFIHQTYLLHPIVLAVILYVVGGIPFLVWGMAVRIVVVLHFTLMVNSVCHIWGK 240

Query: 244 QQWNTSDLSKNN 255
           Q W T D+SKNN
Sbjct: 241 QSWTTGDMSKNN 251

BLAST of Sgr019381 vs. ExPASy Swiss-Prot
Match: Q949X0 (Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ADS3 PE=1 SV=2)

HSP 1 Score: 255.8 bits (652), Expect = 5.3e-67
Identity = 120/231 (51.95%), Postives = 157/231 (67.97%), Query Frame = 0

Query: 25  KWEMLDKNVPFTHLLLLHVPCIFAPFHFNWGAFWVAITLYNLTA-MAISISYHRNLAHRS 84
           +W+ +D       +L +H+  + APF FNW A  VA  LY +T  + I++S+HRNL+H++
Sbjct: 97  EWKAMDFGA-VAVVLSMHLLSLLAPFQFNWRAVSVAFGLYIVTGLLGITLSFHRNLSHKA 156

Query: 85  FKLPKWLEYLLAYCVAHAFQGDPISWVSIHRCHHRYVDTEKDPHSPIQGFWFSHITWLFD 144
           FKLPKWLEYL AYC A A QG+PI WVS HR HH++ D+++DPHSP+ GFWFSH+ W+FD
Sbjct: 157 FKLPKWLEYLFAYCGAQALQGNPIDWVSTHRYHHQFCDSDRDPHSPLDGFWFSHMNWMFD 216

Query: 145 LYTLNEKVCPKYYSDLQKRERNIYMLFFKYGNSDNVTDLEKQTFYRFMQQTYILHPIALA 204
             T+ ++                       G  +NV DLEKQ FYRF++ TYILHP+ALA
Sbjct: 217 TNTITQRC----------------------GEPNNVGDLEKQPFYRFLRTTYILHPLALA 276

Query: 205 LLLYAIGGVPFIIWGVCVRIAVSLQITFLVNSVCHMWGKQQWNTSDLSKNN 255
           + LYA+GG PFI+WG+ VRI     IT+LVNS CH+WGKQ WNT DLSKNN
Sbjct: 277 VALYAMGGFPFIVWGMGVRIVWVYHITWLVNSACHVWGKQAWNTGDLSKNN 304

BLAST of Sgr019381 vs. ExPASy Swiss-Prot
Match: Q9LVZ3 (Probable lipid desaturase ADS3.2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ADS3.2 PE=2 SV=3)

HSP 1 Score: 240.4 bits (612), Expect = 2.3e-62
Identity = 120/245 (48.98%), Postives = 154/245 (62.86%), Query Frame = 0

Query: 14  MVKPPKPFGRSKWEMLD--KNVPF--THLLLLHVPCIFAPFHFNWGAFWVAITLYNLTAM 73
           +V+  + F    W   D  K V F  THLL L      APF+F+W AFWV   L  +  +
Sbjct: 79  VVRTKRSFWERSWTSWDVSKLVIFVGTHLLSL-----LAPFYFSWEAFWVFPWLVFINGI 138

Query: 74  AISISYHRNLAHRSFKLPKWLEYLLAYCVAHAFQGDPISWVSIHRCHHRYVDTEKDPHSP 133
            I++SYHRNL+HRSF LPKWLEYL AY    AFQGDPI WVS HR HH++ +T++DPHSP
Sbjct: 139 CITLSYHRNLSHRSFDLPKWLEYLFAYGGVLAFQGDPIEWVSNHRYHHKHCETQRDPHSP 198

Query: 134 IQGFWFSHITWLFDLYTLNEKVCPKYYSDLQKRERNIYMLFFKYGNSDNVTDLEKQTFYR 193
            QGFWFSH+ W+FD  ++ E                        G  +NV DL +Q FYR
Sbjct: 199 TQGFWFSHMAWIFDTSSILENC----------------------GGEENVDDLVRQPFYR 258

Query: 194 FMQQTYILHPIALALLLYAIGGVPFIIWGVCVRIAVSLQITFLVNSVCHMWGKQQWNTSD 253
           F+Q+T +LH +A + L Y  GG+P ++WG+ + IAV L +TFLVNSVCH+WG + WNTSD
Sbjct: 259 FLQRTVLLHMMAYSFLFYFCGGMPLLVWGIGITIAVRLHLTFLVNSVCHIWGTRAWNTSD 296

Query: 254 LSKNN 255
            SKNN
Sbjct: 319 FSKNN 296

BLAST of Sgr019381 vs. ExPASy Swiss-Prot
Match: Q9SID2 (Delta-9 acyl-lipid desaturase 2 OS=Arabidopsis thaliana OX=3702 GN=ADS2 PE=2 SV=2)

HSP 1 Score: 231.5 bits (589), Expect = 1.1e-59
Identity = 106/233 (45.49%), Postives = 145/233 (62.23%), Query Frame = 0

Query: 21  FGRSKWEMLDKNVPFTHLLLLHVPCIFAPFHFNWGAFWVAITLYNLTAMAISISYHRNLA 80
           F   +W  LD  V F+    +H   + APF+F W A WV    Y +  + I++SYHRNLA
Sbjct: 31  FWDRRWRRLD-YVKFSASFTVHSLALLAPFYFTWSALWVTFLFYTIGGLGITVSYHRNLA 90

Query: 81  HRSFKLPKWLEYLLAYCVAHAFQGDPISWVSIHRCHHRYVDTEKDPHSPIQGFWFSHITW 140
           HRSFK+PKWLEYLLAYC   A QGDPI WVS HR HH++ D+E+DPHSP +GFWFSH+ W
Sbjct: 91  HRSFKVPKWLEYLLAYCALLAIQGDPIDWVSTHRYHHQFTDSERDPHSPKEGFWFSHLLW 150

Query: 141 LFDLYTLNEKVCPKYYSDLQKRERNIYMLFFKYGNSDNVTDLEKQTFYRFMQQTYILHPI 200
           ++D                     + Y L  K G   NV DL++Q FYRF+Q+T + H +
Sbjct: 151 IYD---------------------SAY-LVSKCGRRANVEDLKRQWFYRFLQKTVLFHIL 210

Query: 201 ALALLLYAIGGVPFIIWGVCVRIAVSLQITFLVNSVCHMWGKQQWNTSDLSKN 254
            L   L+ +GG+ F+ WG+ V  A+ + +T L+NS+CH+WG + W T+D S+N
Sbjct: 211 GLGFFLFYLGGMSFVTWGMGVGAALEVHVTCLINSLCHIWGTRTWKTNDTSRN 240

BLAST of Sgr019381 vs. ExPASy Swiss-Prot
Match: P0DOW2 (Acyl-CoA C20 Delta5-desaturase OS=Anemone leveillei OX=212809 GN=AL10 PE=1 SV=1)

HSP 1 Score: 230.7 bits (587), Expect = 1.8e-59
Identity = 113/234 (48.29%), Postives = 143/234 (61.11%), Query Frame = 0

Query: 25  KWEMLDKNVPFTHLLL---LHVPCIFAPFHFNWGAFWVAITLYNLTAM-AISISYHRNLA 84
           KW   D      H+L    LHV C+FAP  F+W +FWV   LY +  +   ++S+HRNL 
Sbjct: 39  KWNSTD----VFHILFIGGLHVLCLFAPSTFSWKSFWVCFALYAICGVFGTTLSFHRNLT 98

Query: 85  HRSFKLPKWLEYLLAYCVAHAFQGDPISWVSIHRCHHRYVDTEKDPHSPIQGFWFSHITW 144
           HRSFKLPK+LEY  AY   HA QGDP+ WVS HR HH+Y DT  DPHSPI+GFWF HI W
Sbjct: 99  HRSFKLPKYLEYFFAYVGLHALQGDPVWWVSTHRYHHKYTDTYLDPHSPIEGFWFCHIFW 158

Query: 145 LFDLYTLNEKVCPKYYSDLQKRERNIYMLFFKYGNSDNVTDLEKQTFYRFMQQTYILHPI 204
           LFD   + EK C +Y                     +N  DL KQ++YRF+++T++ H  
Sbjct: 159 LFDSKYIIEK-CGRY---------------------ENAGDLMKQSYYRFLERTFVYHVY 218

Query: 205 ALALLLYAIGGVPFIIWGVCVRIAVSLQITFLVNSVCHMWGKQQWNTSDLSKNN 255
             A LLY  GG PFI+WG+ VR  +   +++LVNSVCH WG + WNT DLS NN
Sbjct: 219 LQAALLYLFGGFPFIVWGMAVRTILGFHLSWLVNSVCHRWGNRPWNTGDLSTNN 246

BLAST of Sgr019381 vs. ExPASy Swiss-Prot
Match: P0DOW3 (Acyl-CoA 5-desaturase AL21 OS=Anemone leveillei OX=212809 GN=AL21 PE=1 SV=1)

HSP 1 Score: 229.9 bits (585), Expect = 3.1e-59
Identity = 113/234 (48.29%), Postives = 146/234 (62.39%), Query Frame = 0

Query: 26  WEMLDKNVPFTHLLL---LHVPCIFAPFHFNWGAFWVAITLYNLTAM-AISISYHRNLAH 85
           W  +  +    H++L   LHV C+ APF F+W AFW+++TLY +  +   ++SYHRNL H
Sbjct: 33  WVRVWNSTDIFHIILVGGLHVLCLSAPFTFSWSAFWLSLTLYAVCGVFGTTLSYHRNLTH 92

Query: 86  RSFKLPKWLEYLLAYCVAHAFQGDPISWVSIHRCHHRYVDTEKDPHSPIQGFWFSHITWL 145
           RSFKLPK+LEY  AY   HA QGDP+ WVS HR HH++ DT  DPHSPI+GFWFSHI WL
Sbjct: 93  RSFKLPKYLEYFFAYVGLHALQGDPVWWVSTHRYHHKFTDTYLDPHSPIEGFWFSHIFWL 152

Query: 146 FD-LYTLNEKVCPKYYSDLQKRERNIYMLFFKYGNSDNVTDLEKQTFYRFMQQTYILHPI 205
           FD  Y L E  C +Y                     +N  DL KQ++YRF+++T++ H  
Sbjct: 153 FDSKYILEE--CGRY---------------------ENAGDLLKQSYYRFLERTFVFHVY 212

Query: 206 ALALLLYAIGGVPFIIWGVCVRIAVSLQITFLVNSVCHMWGKQQWNTSDLSKNN 255
             A LLY  GG PFI+WG+ VR       ++LVNSVCH +G Q W+T DLS NN
Sbjct: 213 LQAALLYMFGGFPFIVWGMAVRTVWGFHASWLVNSVCHRYGHQAWDTGDLSTNN 243

BLAST of Sgr019381 vs. ExPASy TrEMBL
Match: A0A6J1DQF3 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111022855 PE=3 SV=1)

HSP 1 Score: 399.8 bits (1026), Expect = 8.4e-108
Identity = 186/252 (73.81%), Postives = 207/252 (82.14%), Query Frame = 0

Query: 4   MEASKQEPKKMVK-PPKPFGRSKWEMLDKNVPFTHLLLLHVPCIFAPFHFNWGAFWVAIT 63
           MEASK+EPK+MVK PP PF R    ML +   F   +LLH+ CI APFHFNW AFWVA  
Sbjct: 1   MEASKEEPKEMVKPPPSPFER---RMLRRRTVFAPFILLHLLCILAPFHFNWKAFWVAFG 60

Query: 64  LYNLTAMAISISYHRNLAHRSFKLPKWLEYLLAYCVAHAFQGDPISWVSIHRCHHRYVDT 123
           L   T + IS+SYHRNLAHRSFKLPKWLEYLL YC AHAFQGDPI WVS HRCHHRYVDT
Sbjct: 61  LATTTGLMISVSYHRNLAHRSFKLPKWLEYLLVYCAAHAFQGDPIDWVSTHRCHHRYVDT 120

Query: 124 EKDPHSPIQGFWFSHITWLFDLYTLNEKVCPKYYSDLQKRERNIYMLFFKYGNSDNVTDL 183
           E+DPHSP QGFWFSHITWLFD YTL +KVCP Y +   K E+NI+MLF K+GN DNV DL
Sbjct: 121 ERDPHSPTQGFWFSHITWLFDSYTLTKKVCPNYSNGFGKIEKNIFMLFLKHGNPDNVPDL 180

Query: 184 EKQTFYRFMQQTYILHPIALALLLYAIGGVPFIIWGVCVRIAVSLQITFLVNSVCHMWGK 243
           EKQ FYRFMQ+TY LHPIALA+LLYA+GGVPFIIWG+CVRI VSL ITF+VNSVCH+WG+
Sbjct: 181 EKQVFYRFMQKTYFLHPIALAVLLYAVGGVPFIIWGMCVRITVSLHITFMVNSVCHIWGE 240

Query: 244 QQWNTSDLSKNN 255
           QQW+T DLSKNN
Sbjct: 241 QQWDTGDLSKNN 249

BLAST of Sgr019381 vs. ExPASy TrEMBL
Match: A0A6J1DQF8 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111022861 PE=3 SV=1)

HSP 1 Score: 394.4 bits (1012), Expect = 3.5e-106
Identity = 179/252 (71.03%), Postives = 210/252 (83.33%), Query Frame = 0

Query: 4   MEASKQEPKKMVK-PPKPFGRSKWEMLDKNVPFTHLLLLHVPCIFAPFHFNWGAFWVAIT 63
           MEASKQEPK+MVK P +PF R  W  LDKN+  + L LLH+ CI APFHFNWGAFW+ + 
Sbjct: 1   MEASKQEPKEMVKLPLRPFDRKTWTKLDKNIA-SSLFLLHLLCILAPFHFNWGAFWIFLV 60

Query: 64  LYNLTAMAISISYHRNLAHRSFKLPKWLEYLLAYCVAHAFQGDPISWVSIHRCHHRYVDT 123
           L NLT +AIS+SYH+NLAHRSFKLPKWLEYLLAY  AHAFQGDPI WVS HR HH+YVDT
Sbjct: 61  LTNLTGIAISVSYHKNLAHRSFKLPKWLEYLLAYYAAHAFQGDPIDWVSTHRSHHQYVDT 120

Query: 124 EKDPHSPIQGFWFSHITWLFDLYTLNEKVCPKYYSDLQKRERNIYMLFFKYGNSDNVTDL 183
           E+DPHSP  GFWFSHI WLFD YTL ++VCP Y++D Q+ ERNI+M + K+GN  NV DL
Sbjct: 121 ERDPHSPTNGFWFSHIIWLFDSYTLTKRVCPNYFNDFQEIERNIFMTYLKHGNPSNVHDL 180

Query: 184 EKQTFYRFMQQTYILHPIALALLLYAIGGVPFIIWGVCVRIAVSLQITFLVNSVCHMWGK 243
           EKQ FYRFMQ+TY+LH  ALA+LLY +GGVP+IIWG+C+RI V+L +TF+VNSVCH+WGK
Sbjct: 181 EKQAFYRFMQKTYLLHIFALAILLYFVGGVPYIIWGMCMRIVVTLHLTFMVNSVCHIWGK 240

Query: 244 QQWNTSDLSKNN 255
           QQWNT DLSKNN
Sbjct: 241 QQWNTRDLSKNN 251

BLAST of Sgr019381 vs. ExPASy TrEMBL
Match: A0A6J1DNZ3 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111022863 PE=3 SV=1)

HSP 1 Score: 384.0 bits (985), Expect = 4.8e-103
Identity = 178/252 (70.63%), Postives = 203/252 (80.56%), Query Frame = 0

Query: 4   MEASKQEPKKMVK-PPKPFGRSKWEMLDKNVPFTHLLLLHVPCIFAPFHFNWGAFWVAIT 63
           MEASKQEPK MV+ P +PF R KW  LDKNV  +   +LH+ CI APF+FNWGAFWV + 
Sbjct: 1   MEASKQEPKDMVRLPLRPFERKKWTKLDKNVA-SSFFILHLLCILAPFNFNWGAFWVFLV 60

Query: 64  LYNLTAMAISISYHRNLAHRSFKLPKWLEYLLAYCVAHAFQGDPISWVSIHRCHHRYVDT 123
           + +LT MAIS+SYHRNLAHRSFKLPKWLEYLLAYC AHAFQGDPI WVS HR HHRYVDT
Sbjct: 61  VGSLTGMAISVSYHRNLAHRSFKLPKWLEYLLAYCAAHAFQGDPIDWVSTHRSHHRYVDT 120

Query: 124 EKDPHSPIQGFWFSHITWLFDLYTLNEKVCPKYYSDLQKRERNIYMLFFKYGNSDNVTDL 183
           E+DPHSPI GFWFS ITWLFD Y+L  K CP Y+ D +K ERNI+    KY N  NV DL
Sbjct: 121 ERDPHSPINGFWFSQITWLFDSYSLTTKNCPNYFGDFRKIERNIFTGSLKYANPSNVPDL 180

Query: 184 EKQTFYRFMQQTYILHPIALALLLYAIGGVPFIIWGVCVRIAVSLQITFLVNSVCHMWGK 243
           EKQ FYRFMQ+TY+LH +AL +LLY IGGVP+IIWG+C+RI VS  +T +VNSVCHMWGK
Sbjct: 181 EKQAFYRFMQKTYLLHLLALTILLYVIGGVPYIIWGMCMRIVVSFHLTSMVNSVCHMWGK 240

Query: 244 QQWNTSDLSKNN 255
           +QWNT DLSKNN
Sbjct: 241 KQWNTDDLSKNN 251

BLAST of Sgr019381 vs. ExPASy TrEMBL
Match: A0A6J1DJP0 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111021101 PE=3 SV=1)

HSP 1 Score: 375.6 bits (963), Expect = 1.7e-100
Identity = 165/252 (65.48%), Postives = 204/252 (80.95%), Query Frame = 0

Query: 4   MEASKQEPKKMVKPPKPFGRSKWEMLDKNVPFTHLLLLHVPCIFAPFHFNWGAFWVAITL 63
           MEASKQEPK+M+KPP+P+ R KW  LDK++  + L L+H+ CI APF FNW AFWVA  L
Sbjct: 1   MEASKQEPKEMMKPPRPYERRKWTKLDKDIACS-LFLVHLLCILAPFQFNWSAFWVAFVL 60

Query: 64  YNLTAM-AISISYHRNLAHRSFKLPKWLEYLLAYCVAHAFQGDPISWVSIHRCHHRYVDT 123
           Y +T +  +++SYHRNLAH+SFKLPKWLEYL AYC   A QGDP+ WVS HRCHH++VDT
Sbjct: 61  YIVTGLFGMTLSYHRNLAHKSFKLPKWLEYLFAYCGVQALQGDPMDWVSTHRCHHQFVDT 120

Query: 124 EKDPHSPIQGFWFSHITWLFDLYTLNEKVCPKYYSDLQKRERNIYMLFFKYGNSDNVTDL 183
           E+DPHSPI+GFWFSHI WLFD Y L +KVCPKY+S+ QK ERNI++LF K+G  +NV DL
Sbjct: 121 ERDPHSPIRGFWFSHILWLFDSYNLTKKVCPKYFSNFQKLERNIFVLFSKHGKPENVADL 180

Query: 184 EKQTFYRFMQQTYILHPIALALLLYAIGGVPFIIWGVCVRIAVSLQITFLVNSVCHMWGK 243
           EKQ FYRF+ QTY+LHPI LA++LY +GG+PF++WG+ VRI V L  T +VNSVCH+WGK
Sbjct: 181 EKQAFYRFIHQTYLLHPIVLAVILYVVGGIPFLVWGMAVRIVVVLHFTLMVNSVCHIWGK 240

Query: 244 QQWNTSDLSKNN 255
           Q W T D+SKNN
Sbjct: 241 QSWTTGDMSKNN 251

BLAST of Sgr019381 vs. ExPASy TrEMBL
Match: A0A6J1CR34 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111013452 PE=3 SV=1)

HSP 1 Score: 371.3 bits (952), Expect = 3.2e-99
Identity = 165/253 (65.22%), Postives = 198/253 (78.26%), Query Frame = 0

Query: 4   MEASKQEPKKMVK--PPKPFGRSKWEMLDKNVPFTHLLLLHVPCIFAPFHFNWGAFWVAI 63
           MEASKQEP ++V   PP+ F R KW  LD N   +   L H+PCIFAPF+FNW AFW++ 
Sbjct: 1   MEASKQEPTEVVNKHPPRLFRRKKWTKLDINFAIS-FALFHLPCIFAPFYFNWTAFWISF 60

Query: 64  TLYNLTAMAISISYHRNLAHRSFKLPKWLEYLLAYCVAHAFQGDPISWVSIHRCHHRYVD 123
            L + T +AIS+SYHRNLAHRSFKLPKWLEYLLAY  AHA QGDPI WVS HRCHH+YVD
Sbjct: 61  VLGHSTGIAISVSYHRNLAHRSFKLPKWLEYLLAYWAAHALQGDPIDWVSTHRCHHQYVD 120

Query: 124 TEKDPHSPIQGFWFSHITWLFDLYTLNEKVCPKYYSDLQKRERNIYMLFFKYGNSDNVTD 183
           +E+DPHSPI GFWFSH+ WLFD Y L  KVCP Y+++ QK ER ++  F K+GN  NV D
Sbjct: 121 SERDPHSPIYGFWFSHLIWLFDSYNLTRKVCPNYFNNFQKTERTVFTFFLKHGNPSNVND 180

Query: 184 LEKQTFYRFMQQTYILHPIALALLLYAIGGVPFIIWGVCVRIAVSLQITFLVNSVCHMWG 243
           LEKQ FYRFM++TY LHPI L + LYA+GG+PFIIWG+C+R+ + L +T +VNSVCH+WG
Sbjct: 181 LEKQAFYRFMRRTYFLHPIVLGIFLYAVGGIPFIIWGMCMRVVLLLHVTSMVNSVCHIWG 240

Query: 244 KQQWNTSDLSKNN 255
           KQ WNT DLSKNN
Sbjct: 241 KQPWNTGDLSKNN 252

BLAST of Sgr019381 vs. TAIR 10
Match: AT3G15850.1 (fatty acid desaturase 5 )

HSP 1 Score: 255.8 bits (652), Expect = 3.8e-68
Identity = 120/231 (51.95%), Postives = 157/231 (67.97%), Query Frame = 0

Query: 25  KWEMLDKNVPFTHLLLLHVPCIFAPFHFNWGAFWVAITLYNLTA-MAISISYHRNLAHRS 84
           +W+ +D       +L +H+  + APF FNW A  VA  LY +T  + I++S+HRNL+H++
Sbjct: 97  EWKAMDFGA-VAVVLSMHLLSLLAPFQFNWRAVSVAFGLYIVTGLLGITLSFHRNLSHKA 156

Query: 85  FKLPKWLEYLLAYCVAHAFQGDPISWVSIHRCHHRYVDTEKDPHSPIQGFWFSHITWLFD 144
           FKLPKWLEYL AYC A A QG+PI WVS HR HH++ D+++DPHSP+ GFWFSH+ W+FD
Sbjct: 157 FKLPKWLEYLFAYCGAQALQGNPIDWVSTHRYHHQFCDSDRDPHSPLDGFWFSHMNWMFD 216

Query: 145 LYTLNEKVCPKYYSDLQKRERNIYMLFFKYGNSDNVTDLEKQTFYRFMQQTYILHPIALA 204
             T+ ++                       G  +NV DLEKQ FYRF++ TYILHP+ALA
Sbjct: 217 TNTITQRC----------------------GEPNNVGDLEKQPFYRFLRTTYILHPLALA 276

Query: 205 LLLYAIGGVPFIIWGVCVRIAVSLQITFLVNSVCHMWGKQQWNTSDLSKNN 255
           + LYA+GG PFI+WG+ VRI     IT+LVNS CH+WGKQ WNT DLSKNN
Sbjct: 277 VALYAMGGFPFIVWGMGVRIVWVYHITWLVNSACHVWGKQAWNTGDLSKNN 304

BLAST of Sgr019381 vs. TAIR 10
Match: AT3G15870.1 (Fatty acid desaturase family protein )

HSP 1 Score: 240.4 bits (612), Expect = 1.6e-63
Identity = 120/245 (48.98%), Postives = 154/245 (62.86%), Query Frame = 0

Query: 14  MVKPPKPFGRSKWEMLD--KNVPF--THLLLLHVPCIFAPFHFNWGAFWVAITLYNLTAM 73
           +V+  + F    W   D  K V F  THLL L      APF+F+W AFWV   L  +  +
Sbjct: 79  VVRTKRSFWERSWTSWDVSKLVIFVGTHLLSL-----LAPFYFSWEAFWVFPWLVFINGI 138

Query: 74  AISISYHRNLAHRSFKLPKWLEYLLAYCVAHAFQGDPISWVSIHRCHHRYVDTEKDPHSP 133
            I++SYHRNL+HRSF LPKWLEYL AY    AFQGDPI WVS HR HH++ +T++DPHSP
Sbjct: 139 CITLSYHRNLSHRSFDLPKWLEYLFAYGGVLAFQGDPIEWVSNHRYHHKHCETQRDPHSP 198

Query: 134 IQGFWFSHITWLFDLYTLNEKVCPKYYSDLQKRERNIYMLFFKYGNSDNVTDLEKQTFYR 193
            QGFWFSH+ W+FD  ++ E                        G  +NV DL +Q FYR
Sbjct: 199 TQGFWFSHMAWIFDTSSILENC----------------------GGEENVDDLVRQPFYR 258

Query: 194 FMQQTYILHPIALALLLYAIGGVPFIIWGVCVRIAVSLQITFLVNSVCHMWGKQQWNTSD 253
           F+Q+T +LH +A + L Y  GG+P ++WG+ + IAV L +TFLVNSVCH+WG + WNTSD
Sbjct: 259 FLQRTVLLHMMAYSFLFYFCGGMPLLVWGIGITIAVRLHLTFLVNSVCHIWGTRAWNTSD 296

Query: 254 LSKNN 255
            SKNN
Sbjct: 319 FSKNN 296

BLAST of Sgr019381 vs. TAIR 10
Match: AT2G31360.1 (16:0delta9 desaturase 2 )

HSP 1 Score: 231.5 bits (589), Expect = 7.6e-61
Identity = 106/233 (45.49%), Postives = 145/233 (62.23%), Query Frame = 0

Query: 21  FGRSKWEMLDKNVPFTHLLLLHVPCIFAPFHFNWGAFWVAITLYNLTAMAISISYHRNLA 80
           F   +W  LD  V F+    +H   + APF+F W A WV    Y +  + I++SYHRNLA
Sbjct: 31  FWDRRWRRLD-YVKFSASFTVHSLALLAPFYFTWSALWVTFLFYTIGGLGITVSYHRNLA 90

Query: 81  HRSFKLPKWLEYLLAYCVAHAFQGDPISWVSIHRCHHRYVDTEKDPHSPIQGFWFSHITW 140
           HRSFK+PKWLEYLLAYC   A QGDPI WVS HR HH++ D+E+DPHSP +GFWFSH+ W
Sbjct: 91  HRSFKVPKWLEYLLAYCALLAIQGDPIDWVSTHRYHHQFTDSERDPHSPKEGFWFSHLLW 150

Query: 141 LFDLYTLNEKVCPKYYSDLQKRERNIYMLFFKYGNSDNVTDLEKQTFYRFMQQTYILHPI 200
           ++D                     + Y L  K G   NV DL++Q FYRF+Q+T + H +
Sbjct: 151 IYD---------------------SAY-LVSKCGRRANVEDLKRQWFYRFLQKTVLFHIL 210

Query: 201 ALALLLYAIGGVPFIIWGVCVRIAVSLQITFLVNSVCHMWGKQQWNTSDLSKN 254
            L   L+ +GG+ F+ WG+ V  A+ + +T L+NS+CH+WG + W T+D S+N
Sbjct: 211 GLGFFLFYLGGMSFVTWGMGVGAALEVHVTCLINSLCHIWGTRTWKTNDTSRN 240

BLAST of Sgr019381 vs. TAIR 10
Match: AT1G06080.1 (delta 9 desaturase 1 )

HSP 1 Score: 228.4 bits (581), Expect = 6.4e-60
Identity = 112/259 (43.24%), Postives = 154/259 (59.46%), Query Frame = 0

Query: 4   MEASKQEP--KKMVKPPKPFGRS-------KWEMLDKNVPFTHLLLLHVPCIFAPFHFNW 63
           + AS++E   KKM       GR        KW+ LD    F   L +H  C+ APF+F W
Sbjct: 3   LSASEKEENNKKMAADKAEMGRKKRAMWERKWKRLDIVKAFAS-LFVHFLCLLAPFNFTW 62

Query: 64  GAFWVAITLYNLTAMAISISYHRNLAHRSFKLPKWLEYLLAYCVAHAFQGDPISWVSIHR 123
            A  VA+ +Y +  + I++SYHRNLAHRSFK+PKWLEY  AYC   A QGDPI WVS HR
Sbjct: 63  PALRVALIVYTVGGLGITVSYHRNLAHRSFKVPKWLEYFFAYCGLLAIQGDPIDWVSTHR 122

Query: 124 CHHRYVDTEKDPHSPIQGFWFSHITWLFDLYTLNEKVCPKYYSDLQKRERNIYMLFFKYG 183
            HH++ D+++DPHSP +GFWFSH+ WLFD   L EK                       G
Sbjct: 123 YHHQFTDSDRDPHSPNEGFWFSHLLWLFDTGYLVEKC----------------------G 182

Query: 184 NSDNVTDLEKQTFYRFMQQTYILHPIALALLLYAIGGVPFIIWGVCVRIAVSLQITFLVN 243
              NV DL++Q +Y+F+Q+T + H +    LLY  GG+ F+ WG+ + +A+   +T L+N
Sbjct: 183 RRTNVEDLKRQWYYKFLQRTVLYHILTFGFLLYYFGGLSFLTWGMGIGVAMEHHVTCLIN 238

Query: 244 SVCHMWGKQQWNTSDLSKN 254
           S+CH+WG + W T+D S+N
Sbjct: 243 SLCHVWGSRTWKTNDTSRN 238

BLAST of Sgr019381 vs. TAIR 10
Match: AT1G06120.1 (Fatty acid desaturase family protein )

HSP 1 Score: 216.9 bits (551), Expect = 1.9e-56
Identity = 106/247 (42.91%), Postives = 146/247 (59.11%), Query Frame = 0

Query: 7   SKQEPKKMVKPPKPFGRSKWEMLDKNVPFTHLLLLHVPCIFAPFHFNWGAFWVAITLYNL 66
           S  + K + K  + F   KW  +D  +  + +  +H+ C+ APF++ W AF  A  +   
Sbjct: 9   SSSQSKAVRKEKRAFLFRKWTRVDV-MRVSAVGAVHLLCLLAPFNYTWEAFRFAAMVGIS 68

Query: 67  TAMAISISYHRNLAHRSFKLPKWLEYLLAYCVAHAFQGDPISWVSIHRCHHRYVDTEKDP 126
           T ++I+ SYHRNL HRSFKLPKWLEY  AY    A QG PI WVS HR HH++ D+++DP
Sbjct: 69  TNLSITFSYHRNLTHRSFKLPKWLEYPFAYSALFALQGHPIDWVSTHRFHHQFTDSDRDP 128

Query: 127 HSPIQGFWFSHITWLFDLYTLNEKVCPKYYSDLQKRERNIYMLFFKYGNSDNVTDLEKQT 186
           HSPI+GFWFSH+ W+FD   + EK                       G  DNV DL++Q 
Sbjct: 129 HSPIEGFWFSHVFWIFDTSYIREKC----------------------GGRDNVMDLKQQW 188

Query: 187 FYRFMQQTYILHPIALALLLYAIGGVPFIIWGVCVRIAVSLQITFLVNSVCHMWGKQQWN 246
           FYRF+Q T  LH +   +L+Y  GG+P++ W V V  A+    T+L+NS CH+WG + WN
Sbjct: 189 FYRFLQNTIGLHILTFWILVYLWGGLPYLTWSVGVGGAIGYHATWLINSACHIWGSRAWN 232

Query: 247 TSDLSKN 254
           T D S+N
Sbjct: 249 TKDTSRN 232