Sgr019171 (gene) Monk fruit (Qingpiguo) v1

BLAST of Sgr019171 vs. NCBI nr
Match: XP_008439665.1 (PREDICTED: two-pore potassium channel 3 [Cucumis melo])

HSP 1 Score: 782.3 bits (2019), Expect = 2.1e-222
Identity = 394/425 (92.71%), Postives = 414/425 (97.41%), Query Frame = 0

Query: 1   MDKEPLLPYLSPRGKPPPPPPQLCPLPEHDEITLPLTTTEFKDRLIFGPSSSPQDASPIF 60
           M+KEPLLPYLSPRGKPPPPPPQLCPLPE+DEITLP+T TEFKDRLIFGPSSSPQDASPIF
Sbjct: 1   MEKEPLLPYLSPRGKPPPPPPQLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDASPIF 60

Query: 61  DALTLSLSSSRPSASSSLQDPSTLLDPKSQSQSQQAWLIDSNYSWPKTNLHRSKTAPAMA 120
           DALTLSLSSSRPSASSSLQDPST LDP+SQSQSQQAWL+DSNY+WPK+NLHRSKTAPAMA
Sbjct: 61  DALTLSLSSSRPSASSSLQDPSTPLDPRSQSQSQQAWLVDSNYTWPKSNLHRSKTAPAMA 120

Query: 121 VINDVNHSHDPKPQFGKQSIIRQAVVLLIIYLSLGVVIYWINRDNFSAHETHPVVDALYF 180
           VINDVNHS +PKPQFGKQSIIRQAVVLLI+YLSLGV+IYW+NRDNF+A ETHPVVDALYF
Sbjct: 121 VINDVNHSQEPKPQFGKQSIIRQAVVLLIVYLSLGVLIYWLNRDNFAAQETHPVVDALYF 180

Query: 181 CIVTMCTIGYGDITPNSAATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLKNVKR 240
           CIVTMCTIGYGDITPNS ATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLL+NVKR
Sbjct: 181 CIVTMCTIGYGDITPNSTATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKR 240

Query: 241 GAQPESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGTGVMHFIENLGWLDSFYLSVM 300
           G + ESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVG GVMHF+ENLGWLDSFYLSVM
Sbjct: 241 GVKRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLDSFYLSVM 300

Query: 301 SVTTVGYGDQAFKSMPGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRKMAKWILGQDM 360
           SVTTVGYGDQAFKSM GRIFASIWLLVSTLAVARAFLYLAEARVDKRHR MAKWILGQDM
Sbjct: 301 SVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDM 360

Query: 361 TVSEFLAADIDNNGFV-RSEYVVYKLKEMGKVSEKDIMQISNNFDRLDTGKCGKITLADL 420
           T+SEFLAADIDNNGFV RSEYV+YKLKEMG+VSEKD+MQISNNFDRLD+GKCGKITLADL
Sbjct: 361 TISEFLAADIDNNGFVSRSEYVIYKLKEMGRVSEKDVMQISNNFDRLDSGKCGKITLADL 420

Query: 421 LESHH 425
           LESHH
Sbjct: 421 LESHH 425

BLAST of Sgr019171 vs. NCBI nr
Match: XP_038883350.1 (two-pore potassium channel 3-like [Benincasa hispida])

HSP 1 Score: 777.3 bits (2006), Expect = 6.6e-221
Identity = 395/425 (92.94%), Postives = 410/425 (96.47%), Query Frame = 0

Query: 1   MDKEPLLPYLSPRGKPPPPPPQLCPLPEHDEITLPLTTTEFKDRLIFGPSSSPQDASPIF 60
           M+KEPLLPY+SPRGKPPPPP QLCPLPE+DEITLP+T TEFKDRLIFGPSSSPQDASPIF
Sbjct: 1   MEKEPLLPYVSPRGKPPPPPAQLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDASPIF 60

Query: 61  DALTLSLSSSRPSASSSLQDPSTLLDPKSQSQSQQAWLIDSNYSWPKTNLHRSKTAPAMA 120
           DALTLSLSSSRPSASSSLQDPST LD KSQSQSQQAWL+DSNY+WPK+NLHRSKTAPAMA
Sbjct: 61  DALTLSLSSSRPSASSSLQDPSTPLDLKSQSQSQQAWLVDSNYTWPKSNLHRSKTAPAMA 120

Query: 121 VINDVNHSHDPKPQFGKQSIIRQAVVLLIIYLSLGVVIYWINRDNFSAHETHPVVDALYF 180
           VINDVNHS +PKPQFGKQSIIRQAVVLLI+YLSLGV IYW+NRD FSAHETHPVVDALYF
Sbjct: 121 VINDVNHSQEPKPQFGKQSIIRQAVVLLIVYLSLGVFIYWLNRDKFSAHETHPVVDALYF 180

Query: 181 CIVTMCTIGYGDITPNSAATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLKNVKR 240
           CIVTMCTIGYGDITPNS ATKLFSVLFVLVGFGFIDILLSGMVSY+LDLQESYLL+NVKR
Sbjct: 181 CIVTMCTIGYGDITPNSTATKLFSVLFVLVGFGFIDILLSGMVSYMLDLQESYLLRNVKR 240

Query: 241 GAQPESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGTGVMHFIENLGWLDSFYLSVM 300
           G Q ESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVG  VMHF+ENLGWLDSFYLSVM
Sbjct: 241 GLQRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMSVMHFVENLGWLDSFYLSVM 300

Query: 301 SVTTVGYGDQAFKSMPGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRKMAKWILGQDM 360
           SVTTVGYGDQAFKSM GRIFASIWLLVSTLAVARAFLYLAEARVDKRHR MAKWILGQDM
Sbjct: 301 SVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDM 360

Query: 361 TVSEFLAADIDNNGFV-RSEYVVYKLKEMGKVSEKDIMQISNNFDRLDTGKCGKITLADL 420
           TVSEFLAADIDNNGFV RSEYV+YKLKEMGKVSEKD+MQISNNFDRLDTGKCGKITLADL
Sbjct: 361 TVSEFLAADIDNNGFVSRSEYVIYKLKEMGKVSEKDVMQISNNFDRLDTGKCGKITLADL 420

Query: 421 LESHH 425
           LESHH
Sbjct: 421 LESHH 425

BLAST of Sgr019171 vs. NCBI nr
Match: XP_004134597.1 (two-pore potassium channel 3 [Cucumis sativus])

HSP 1 Score: 775.8 bits (2002), Expect = 1.9e-220
Identity = 393/425 (92.47%), Postives = 412/425 (96.94%), Query Frame = 0

Query: 1   MDKEPLLPYLSPRGKPPPPPPQLCPLPEHDEITLPLTTTEFKDRLIFGPSSSPQDASPIF 60
           M+KEPLLPYLSPRGKP P PPQLCPLPE+DEITLP+T TEFKDRLIFGPSSSPQDASPIF
Sbjct: 1   MEKEPLLPYLSPRGKPSPIPPQLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDASPIF 60

Query: 61  DALTLSLSSSRPSASSSLQDPSTLLDPKSQSQSQQAWLIDSNYSWPKTNLHRSKTAPAMA 120
           DALTLSLSSSRPSASSSLQDPST LDP+SQSQSQQAWL+DSNY+WPK+NLHRS+TAPAMA
Sbjct: 61  DALTLSLSSSRPSASSSLQDPSTPLDPRSQSQSQQAWLVDSNYTWPKSNLHRSRTAPAMA 120

Query: 121 VINDVNHSHDPKPQFGKQSIIRQAVVLLIIYLSLGVVIYWINRDNFSAHETHPVVDALYF 180
           VINDVNHS +PKPQFGKQSIIRQAVVLLI+YLSLGV+IYW+NRDNFSA ETHPVVDALYF
Sbjct: 121 VINDVNHSQEPKPQFGKQSIIRQAVVLLIVYLSLGVLIYWLNRDNFSAQETHPVVDALYF 180

Query: 181 CIVTMCTIGYGDITPNSAATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLKNVKR 240
           CIVTMCTIGYGDITPNS +TKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLL+NVKR
Sbjct: 181 CIVTMCTIGYGDITPNSTSTKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKR 240

Query: 241 GAQPESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGTGVMHFIENLGWLDSFYLSVM 300
           G + ESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVG GVMHF+ENLGWLDSFYLSVM
Sbjct: 241 GVKRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLDSFYLSVM 300

Query: 301 SVTTVGYGDQAFKSMPGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRKMAKWILGQDM 360
           SVTTVGYGDQAFKSM GRIFASIWLLVSTLAVARAFLYLAEARVDKRHR MAKWILGQDM
Sbjct: 301 SVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDM 360

Query: 361 TVSEFLAADIDNNGFV-RSEYVVYKLKEMGKVSEKDIMQISNNFDRLDTGKCGKITLADL 420
           TVSEFLAADIDNNGFV RSEYV+YKLKEMGKVSEKD+MQISNNFDRLD+GKCGKITLADL
Sbjct: 361 TVSEFLAADIDNNGFVSRSEYVIYKLKEMGKVSEKDVMQISNNFDRLDSGKCGKITLADL 420

Query: 421 LESHH 425
           LESHH
Sbjct: 421 LESHH 425

BLAST of Sgr019171 vs. NCBI nr
Match: XP_023544618.1 (two-pore potassium channel 3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 764.6 bits (1973), Expect = 4.5e-217
Identity = 393/426 (92.25%), Postives = 407/426 (95.54%), Query Frame = 0

Query: 1   MDKEPLLPYLSPRGKPPPPPPQ-LCPLPEHDEITLPLTTTEFKDRLIFGPSSSPQDASPI 60
           M+KEPLLPY+SPRGKP PPPPQ LCPLPE+DEITLP+T TEFKDRLIFGPSSSPQD SPI
Sbjct: 1   MEKEPLLPYMSPRGKPLPPPPQLLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDTSPI 60

Query: 61  FDALTLSLSSSRPSASSSLQDPSTLLDPKSQSQSQQAWLIDSNYSWPKTNLHRSKTAPAM 120
           FDALTLSLSSSRPSASSSLQDPST L PKSQS SQQAWL+DSNYSW K+NLHRSKTAPAM
Sbjct: 61  FDALTLSLSSSRPSASSSLQDPSTPLAPKSQSPSQQAWLVDSNYSWHKSNLHRSKTAPAM 120

Query: 121 AVINDVNHSHDPKPQFGKQSIIRQAVVLLIIYLSLGVVIYWINRDNFSAHETHPVVDALY 180
           AVINDVNHS +PKPQFGKQSIIRQAVVLLI+YLSLGV+IYWINRD FSAHETHPVVDALY
Sbjct: 121 AVINDVNHSQEPKPQFGKQSIIRQAVVLLILYLSLGVLIYWINRDKFSAHETHPVVDALY 180

Query: 181 FCIVTMCTIGYGDITPNSAATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLKNVK 240
           FCIVTMCTIGYGDITPNSAATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLL+NVK
Sbjct: 181 FCIVTMCTIGYGDITPNSAATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVK 240

Query: 241 RGAQPESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGTGVMHFIENLGWLDSFYLSV 300
           RG Q ESGKSYIIDVKKGRMRIRMKVALALGVVILCIG+G GVMHF+ENLGWLDSFYLSV
Sbjct: 241 RGVQRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGLGMGVMHFVENLGWLDSFYLSV 300

Query: 301 MSVTTVGYGDQAFKSMPGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRKMAKWILGQD 360
           MSVTTVGYGDQAFKSM GRIFASIWLLVSTLAVARAFLYLAEARVDKRHR MAKWILGQD
Sbjct: 301 MSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQD 360

Query: 361 MTVSEFLAADIDNNGFV-RSEYVVYKLKEMGKVSEKDIMQISNNFDRLDTGKCGKITLAD 420
           MTVSEFLAAD DNNG V RSEYV+YKLKEMGKVSEKD+MQISNNFDRLDTG CGKITLAD
Sbjct: 361 MTVSEFLAADTDNNGCVSRSEYVLYKLKEMGKVSEKDVMQISNNFDRLDTGNCGKITLAD 420

Query: 421 LLESHH 425
           LLESHH
Sbjct: 421 LLESHH 426

BLAST of Sgr019171 vs. NCBI nr
Match: XP_022926002.1 (two-pore potassium channel 3-like [Cucurbita moschata])

HSP 1 Score: 764.6 bits (1973), Expect = 4.5e-217
Identity = 393/426 (92.25%), Postives = 407/426 (95.54%), Query Frame = 0

Query: 1   MDKEPLLPYLSPRGKPPPPPPQ-LCPLPEHDEITLPLTTTEFKDRLIFGPSSSPQDASPI 60
           M+KEPLLPY+SPRGKP PPPPQ LCPLPE+DEITLP+T TEFKDRLIFGPSSSPQD SPI
Sbjct: 1   MEKEPLLPYISPRGKPLPPPPQLLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDTSPI 60

Query: 61  FDALTLSLSSSRPSASSSLQDPSTLLDPKSQSQSQQAWLIDSNYSWPKTNLHRSKTAPAM 120
           FDALTLSLSSSRPSASSSLQDPST L PKSQS SQQAWL+DSNYSW K+NLHRSKTAPAM
Sbjct: 61  FDALTLSLSSSRPSASSSLQDPSTPLAPKSQSPSQQAWLVDSNYSWHKSNLHRSKTAPAM 120

Query: 121 AVINDVNHSHDPKPQFGKQSIIRQAVVLLIIYLSLGVVIYWINRDNFSAHETHPVVDALY 180
           AVINDVNHS +PKPQFGKQSIIRQAVVLLI+YLSLGV+IYWINRD FSAHETHPVVDALY
Sbjct: 121 AVINDVNHSQEPKPQFGKQSIIRQAVVLLILYLSLGVLIYWINRDKFSAHETHPVVDALY 180

Query: 181 FCIVTMCTIGYGDITPNSAATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLKNVK 240
           FCIVTMCTIGYGDITPNSAATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLL+NVK
Sbjct: 181 FCIVTMCTIGYGDITPNSAATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVK 240

Query: 241 RGAQPESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGTGVMHFIENLGWLDSFYLSV 300
           RG Q ESGKSYIIDVKKGRMRIRMKVALALGVVILCIG+G GVMHF+ENLGWLDSFYLSV
Sbjct: 241 RGVQRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGLGMGVMHFVENLGWLDSFYLSV 300

Query: 301 MSVTTVGYGDQAFKSMPGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRKMAKWILGQD 360
           MSVTTVGYGDQAFKSM GRIFASIWLLVSTLAVARAFLYLAEARVDKRHR MAKWILGQD
Sbjct: 301 MSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQD 360

Query: 361 MTVSEFLAADIDNNGFV-RSEYVVYKLKEMGKVSEKDIMQISNNFDRLDTGKCGKITLAD 420
           MTVSEFLAAD DNNG V RSEYV+YKLKEMGKVSEKD+MQISNNFDRLDTG CGKITLAD
Sbjct: 361 MTVSEFLAADTDNNGCVSRSEYVLYKLKEMGKVSEKDVMQISNNFDRLDTGNCGKITLAD 420

Query: 421 LLESHH 425
           LLESHH
Sbjct: 421 LLESHH 426

BLAST of Sgr019171 vs. ExPASy Swiss-Prot
Match: Q9SVV6 (Two-pore potassium channel 3 OS=Arabidopsis thaliana OX=3702 GN=TPK3 PE=2 SV=1)

HSP 1 Score: 503.8 bits (1296), Expect = 1.9e-141
Identity = 276/427 (64.64%), Postives = 336/427 (78.69%), Query Frame = 0

Query: 4   EPLLPY-LSPRGKPPPPPPQLCPLPEHDE--ITLPLTTTEFKDRLIFGP-SSSPQDASPI 63
           +PLL Y +SPR K   PP  L PLPE +E  I +P+T +EFK+RLIFGP S SP+D+S  
Sbjct: 7   DPLLQYMISPRLK--KPPQLLFPLPEDNEVAIPMPMTPSEFKERLIFGPFSCSPRDSSHF 66

Query: 64  FDALTLSLSSSRPSASSSLQDPSTLLDP--KSQSQSQQAWLIDSNYSWPKTN-LHRSKTA 123
            D++     SS  +A ++    S+ LDP         + WL D   S  + + LHRSKTA
Sbjct: 67  IDSMKQPSPSSSSTAVNNPFSDSSTLDPLLPPPPPQPEPWLSDQTSSHCQGHALHRSKTA 126

Query: 124 PAMAVINDVNHSHDPK-PQFGKQSIIRQAVVLLIIYLSLGVVIYWINRDNFSAHETHPVV 183
           PAMAVIND++H    K P    +S++RQA  LL++YLSLGV+IYW+NRD++  ++THPVV
Sbjct: 127 PAMAVINDLHHPIRQKDPTETSRSVVRQAFALLVVYLSLGVLIYWLNRDHYVVNQTHPVV 186

Query: 184 DALYFCIVTMCTIGYGDITPNSAATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLL 243
           D LYFCIVTMCTIGYGDITPNS  TKLFS++FVLVGFGFIDILLSGMVSYVLDLQESY+L
Sbjct: 187 DGLYFCIVTMCTIGYGDITPNSVVTKLFSIMFVLVGFGFIDILLSGMVSYVLDLQESYML 246

Query: 244 KNVKRGAQPESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGTGVMHFIENLGWLDSF 303
            + KR  +PE  +SYIIDVKKGRMRIR+KVALALGVV+LCI VG G+MHFIE +GWLDSF
Sbjct: 247 DSAKRRDEPEKRRSYIIDVKKGRMRIRLKVALALGVVVLCIAVGVGIMHFIEEIGWLDSF 306

Query: 304 YLSVMSVTTVGYGDQAFKSMPGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRKMAKWI 363
           YLSVMSVTTVGYGD+AFK++PGR+FA+IWLLVSTLAVARAFLYLAEARVDKR+R+ AK +
Sbjct: 307 YLSVMSVTTVGYGDRAFKTLPGRLFAAIWLLVSTLAVARAFLYLAEARVDKRNRERAKKV 366

Query: 364 LGQDMTVSEFLAADIDNNGFV-RSEYVVYKLKEMGKVSEKDIMQISNNFDRLDTGKCGKI 422
           L + M+VS+F AADIDNNG V ++EYV+YKLKEM K+++KDI+ IS  FD+LD    GKI
Sbjct: 367 LCETMSVSQFFAADIDNNGCVSKAEYVIYKLKEMEKITDKDILPISKQFDKLDRCSNGKI 426

BLAST of Sgr019171 vs. ExPASy Swiss-Prot
Match: Q9FL25 (Two-pore potassium channel 2 OS=Arabidopsis thaliana OX=3702 GN=TPK2 PE=2 SV=1)

HSP 1 Score: 453.8 bits (1166), Expect = 2.2e-126
Identity = 249/430 (57.91%), Postives = 325/430 (75.58%), Query Frame = 0

Query: 4   EPLLPYL-SPRGKPPPPPPQLCPLPEHDEIT--LPLTTTEFKDRLIFGP-SSSPQDASPI 63
           +PL  YL +PR    PPPP L  LPE++++T  +P+T  E K+RLIFG    S +++S  
Sbjct: 12  DPLRQYLMNPRIN--PPPPSLLTLPENNDVTIPMPITPLELKNRLIFGSFVRSRKESSLP 71

Query: 64  FDALTLSLSSSRPSASSSLQDPSTLLDPKSQSQSQQAWLIDSNYSWPKTNLHRSKTAPAM 123
            DAL+ + S+S  SA++S  D + LL P ++             S P  N HRSKTAPAM
Sbjct: 72  IDALSQNPSTS-SSATTSFSDSTDLLLPLTEPNK------PVRKSKPTINFHRSKTAPAM 131

Query: 124 AVINDVNHSHDPK--PQFGKQSIIRQAVVLLIIYLSLGVVIYWINRDNFSAHETHPVVDA 183
           A IN+++H +DPK   Q   ++I+ QAV LL++YLSLGV+IYW+NRD+++  +THPVVDA
Sbjct: 132 AAINNISHPNDPKTDQQSDSKTIVNQAVALLVVYLSLGVLIYWLNRDSYNVKQTHPVVDA 191

Query: 184 LYFCIVTMCTIGYGDITPNSAATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLKN 243
           LYFCIVTMCTIGYGDITP+S  TKLFS+ FVLVGFGF+DILLSGMV+YVLDLQE+Y+L+ 
Sbjct: 192 LYFCIVTMCTIGYGDITPDSVVTKLFSIFFVLVGFGFMDILLSGMVTYVLDLQENYMLET 251

Query: 244 VKRGA----QPESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGTGVMHFIENLGWLD 303
            +  +      +  +SYIIDVKKGRMRIR+KV LALGVV+LC+G G  +MHF+E +GWLD
Sbjct: 252 ARNESLNLNDRDKVRSYIIDVKKGRMRIRLKVGLALGVVVLCLGFGVLIMHFVEKIGWLD 311

Query: 304 SFYLSVMSVTTVGYGDQAFKSMPGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRKMAK 363
           SFY SVMSVTTVGYGD+AF ++ GR+ A++WLLVSTLAVARA L+LAE+RVDKR+R+ AK
Sbjct: 312 SFYFSVMSVTTVGYGDRAFNTLAGRLLAAMWLLVSTLAVARAILFLAESRVDKRNRERAK 371

Query: 364 WILGQDMTVSEFLAADIDNNGFV-RSEYVVYKLKEMGKVSEKDIMQISNNFDRLDTGKCG 423
            +LG+ M++S+FL ADID NG V ++E+V+YKLK+M K++EKDI  I   FD+LD    G
Sbjct: 372 KVLGESMSISQFLDADIDCNGCVSKAEFVIYKLKKMDKITEKDINPIGFQFDKLDRTNSG 431

BLAST of Sgr019171 vs. ExPASy Swiss-Prot
Match: Q69TN4 (Two pore potassium channel c OS=Oryza sativa subsp. japonica OX=39947 GN=TPKC PE=3 SV=1)

HSP 1 Score: 425.6 bits (1093), Expect = 6.4e-118
Identity = 250/453 (55.19%), Postives = 302/453 (66.67%), Query Frame = 0

Query: 1   MDKEPLLPYLSPRGKPPPPPPQLCPLPEHDEIT--------LPLTTTEFKDRLIFG---- 60
           MD EPLL  LS      P P  L PLPEH E++         P   + +K+R+IFG    
Sbjct: 1   MDTEPLLSPLS------PSPHLLHPLPEHAEVSTFSPPLSPCPSPASSYKERIIFGAHPP 60

Query: 61  ---PSSSPQDASPIFDALTLSLSSSR---PSASSSLQDPSTLLDPKSQSQSQQAWLIDSN 120
              P   P    P        +S      PS SSS   PS   +P     S    L D  
Sbjct: 61  PPPPPPPPPPPPPRGRRYYRRVSGDDLDVPSCSSSPSPPSDEENPPPNPPS----LFD-- 120

Query: 121 YSWPKTNLHRSKTAPAMAVINDV--------NHSHDPKPQFGKQSIIRQAVVLLIIYLSL 180
           +   +TNLHRS+TAPAMA +N            S +P P   + +I+  A + L+ YL++
Sbjct: 121 FIGGRTNLHRSRTAPAMAPLNAAAIAAAAASGDSRNPPPPPRRPAIVLHAFLFLLAYLAM 180

Query: 181 GVVIYWINRDNF--SAHETHPVVDALYFCIVTMCTIGYGDITPNSAATKLFSVLFVLVGF 240
           GV  Y     NF  SA  THPV DALYFCIVT+CTIGYGDITP + A KLFS+ FVL+GF
Sbjct: 181 GVTFYAALPGNFTSSAGPTHPVADALYFCIVTLCTIGYGDITPATPAAKLFSISFVLIGF 240

Query: 241 GFIDILLSGMVSYVLDLQESYLLKNVKRGAQPESGK-SYIIDVKKGRMRIRMKVALALGV 300
           GF+DILLSGMVSYVLDLQE  L+  +K        + +YI D+KKGRMR+RMKVALAL V
Sbjct: 241 GFVDILLSGMVSYVLDLQEHLLITALKNPRSVRKHRHNYIFDLKKGRMRVRMKVALALTV 300

Query: 301 VILCIGVGTGVMHFIENLGWLDSFYLSVMSVTTVGYGDQAFKSMPGRIFASIWLLVSTLA 360
           V +C+GVG  V+  +ENLGWLD+ YL+VMSVTTVGYGD AF+++ GR+FAS WLLVSTLA
Sbjct: 301 VAICVGVGAAVLKRVENLGWLDAVYLAVMSVTTVGYGDHAFRTLAGRLFASAWLLVSTLA 360

Query: 361 VARAFLYLAEARVDKRHRKMAKWILGQDMTVSEFLAADIDNNGFV-RSEYVVYKLKEMGK 420
           VARAFLYLAE R+DKRHR MA W+L +DMTVSEFLAADIDNNG+V +SE+VVYKLKEMGK
Sbjct: 361 VARAFLYLAEMRIDKRHRAMANWVLSRDMTVSEFLAADIDNNGYVTKSEFVVYKLKEMGK 420

Query: 421 VSEKDIMQISNNFDRLDTGKCGKITLADLLESH 424
           +SEKDIM I + F R+D+G CGKITL+DLLESH
Sbjct: 421 ISEKDIMMICDQFQRMDSGNCGKITLSDLLESH 441

BLAST of Sgr019171 vs. ExPASy Swiss-Prot
Match: Q9S6Z8 (Two-pore potassium channel 5 OS=Arabidopsis thaliana OX=3702 GN=TPK5 PE=1 SV=1)

HSP 1 Score: 382.1 bits (980), Expect = 8.2e-105
Identity = 225/422 (53.32%), Postives = 283/422 (67.06%), Query Frame = 0

Query: 17  PPPPPQLCPLPEHDEITLPLTTTEFKDRLIFGPSSSPQDASPIFDALTLSLSSSRPSASS 76
           P P  +L P+PE+                   PSSS   AS     +T+  S S  S   
Sbjct: 7   PQPRFRLQPIPEN-------------------PSSSSSSAS-----ITIPRSISNTSFFH 66

Query: 77  SLQDPSTLLDPKSQSQSQQAWLIDSNYSWPKTN--------LHRSKTAPAMAVINDVNHS 136
            +     LL  +   QS Q    D +    +TN        LHRS+TAPAM +I D+   
Sbjct: 67  EISQERLLLHHQDLEQSVQDDKEDQDSDSDETNRFLSQTRPLHRSRTAPAMVIIKDLRTK 126

Query: 137 --HDPKPQFGKQSIIRQAVVLLIIYLSLGVVIYWINRDNFSAHETHPVVDALYFCIVTMC 196
                KP    +SIIRQA+ LLI+YL+LGV IY  NRD++S  ETHPVVDALYFCIVTMC
Sbjct: 127 PPETKKPSPVSKSIIRQAIFLLIVYLTLGVSIYSFNRDHYSGIETHPVVDALYFCIVTMC 186

Query: 197 TIGYGDITPNSAATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLKNVKRGAQPE- 256
           TIGYGDI P +  TK+F+V+FVL GFGF+DILLSG+V+YVLDLQES +L  ++     + 
Sbjct: 187 TIGYGDIAPLTPWTKIFAVVFVLFGFGFLDILLSGVVNYVLDLQESMILTGIQTRQHHQH 246

Query: 257 ------SGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGTGVMHFIENLGWLDSFYLSV 316
                 S K YIID +KGRMRIRMKV LAL VV+LCIGVG  V+HF+E LG++DS YLSV
Sbjct: 247 HHHHRFSAKDYIIDFEKGRMRIRMKVCLALCVVVLCIGVGALVLHFVEELGFVDSVYLSV 306

Query: 317 MSVTTVGYGDQAFKSMPGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRKMAKWILGQD 376
           MSVTTVGYGD+AFK++ GR+FA++WLLVSTLAVARAFLYLAEAR+D+RHRK  K  L ++
Sbjct: 307 MSVTTVGYGDRAFKTLQGRLFAAVWLLVSTLAVARAFLYLAEARIDRRHRKAVKLALNRE 366

Query: 377 MTVSEFLAADIDNNGFV-RSEYVVYKLKEMGKVSEKDIMQISNNFDRLDTGKCGKITLAD 421
           +TV + L AD   +GF+ +SEY+V KLKEMGK+++KDI Q+   F++LD  + GKITL D
Sbjct: 367 ITVDDLLKADTYQHGFISKSEYIVLKLKEMGKITQKDIDQVVIQFEKLDPNQIGKITLPD 404

BLAST of Sgr019171 vs. ExPASy Swiss-Prot
Match: Q8LBL1 (Two-pore potassium channel 1 OS=Arabidopsis thaliana OX=3702 GN=TPK1 PE=1 SV=2)

HSP 1 Score: 213.4 bits (542), Expect = 5.0e-54
Identity = 124/344 (36.05%), Postives = 206/344 (59.88%), Query Frame = 0

Query: 88  KSQSQSQQAWLIDSNYSWPKTNLHRSKTAP-AMAVINDVNHSHDPKPQFGKQSI------ 147
           K  + +Q   L     S  K  L RS++AP    + ND     +P P   K  +      
Sbjct: 15  KIDTMAQDFNLNSRTSSSRKRRLRRSRSAPRGDCMYNDDVKIDEPPPHPSKIPMFSDLNP 74

Query: 148 -IRQAVVLLIIYLSLGVVIYWINRDNFSAHETHPVVDALYFCIVTMCTIGYGDITPNSAA 207
            +R+ ++ L +YL++G + +++ RD  S H+T  VVDALYFCIVTM T+GYGD+ PNS+A
Sbjct: 75  NLRRVIMFLALYLTIGTLCFYLVRDQISGHKTSGVVDALYFCIVTMTTVGYGDLVPNSSA 134

Query: 208 TKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLK--NVKRGAQPESGKSYIIDVKKG 267
           ++L +  FV  G   +  LLS    Y+++ QE+ L++  ++++   P      + ++   
Sbjct: 135 SRLLACAFVFSGMVLVGHLLSRAADYLVEKQEALLVRAFHLRQSFGP---TDILKELHTN 194

Query: 268 RMRIRMKVALALGVVILCIGVGTGVMHFIENLGWLDSFYLSVMSVTTVGYGDQAFKSMPG 327
           ++R +   A  L +V+L I VGT  +  +E +  + +FY    +VTT+GYGD++F S  G
Sbjct: 195 KLRYKC-YATCLVLVVLFI-VGTIFLVMVEKMPVISAFYCVCSTVTTLGYGDKSFNSEAG 254

Query: 328 RIFASIWLLVSTLAVARAFLYLAEARVDKRHRKMAKWILGQDMTVSEFLAADIDNNGFV- 387
           R+FA  W+L S++ +A+ FLY+AE   + + R + KW+L + +T ++  AAD+D +G V 
Sbjct: 255 RLFAVFWILTSSICLAQFFLYVAELNTENKQRALVKWVLTRRITNNDLEAADLDEDGVVG 314

Query: 388 RSEYVVYKLKEMGKVSEKDIMQISNNFDRLDTGKCGKITLADLL 421
            +E++VYKLKEMGK+ EKDI  I + F++LD  + G +T +D++
Sbjct: 315 AAEFIVYKLKEMGKIDEKDISGIMDEFEQLDYDESGTLTTSDIV 353

BLAST of Sgr019171 vs. ExPASy TrEMBL
Match: A0A1S3AYW0 (two-pore potassium channel 3 OS=Cucumis melo OX=3656 GN=LOC103484391 PE=3 SV=1)

HSP 1 Score: 782.3 bits (2019), Expect = 1.0e-222
Identity = 394/425 (92.71%), Postives = 414/425 (97.41%), Query Frame = 0

Query: 1   MDKEPLLPYLSPRGKPPPPPPQLCPLPEHDEITLPLTTTEFKDRLIFGPSSSPQDASPIF 60
           M+KEPLLPYLSPRGKPPPPPPQLCPLPE+DEITLP+T TEFKDRLIFGPSSSPQDASPIF
Sbjct: 1   MEKEPLLPYLSPRGKPPPPPPQLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDASPIF 60

Query: 61  DALTLSLSSSRPSASSSLQDPSTLLDPKSQSQSQQAWLIDSNYSWPKTNLHRSKTAPAMA 120
           DALTLSLSSSRPSASSSLQDPST LDP+SQSQSQQAWL+DSNY+WPK+NLHRSKTAPAMA
Sbjct: 61  DALTLSLSSSRPSASSSLQDPSTPLDPRSQSQSQQAWLVDSNYTWPKSNLHRSKTAPAMA 120

Query: 121 VINDVNHSHDPKPQFGKQSIIRQAVVLLIIYLSLGVVIYWINRDNFSAHETHPVVDALYF 180
           VINDVNHS +PKPQFGKQSIIRQAVVLLI+YLSLGV+IYW+NRDNF+A ETHPVVDALYF
Sbjct: 121 VINDVNHSQEPKPQFGKQSIIRQAVVLLIVYLSLGVLIYWLNRDNFAAQETHPVVDALYF 180

Query: 181 CIVTMCTIGYGDITPNSAATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLKNVKR 240
           CIVTMCTIGYGDITPNS ATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLL+NVKR
Sbjct: 181 CIVTMCTIGYGDITPNSTATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKR 240

Query: 241 GAQPESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGTGVMHFIENLGWLDSFYLSVM 300
           G + ESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVG GVMHF+ENLGWLDSFYLSVM
Sbjct: 241 GVKRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLDSFYLSVM 300

Query: 301 SVTTVGYGDQAFKSMPGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRKMAKWILGQDM 360
           SVTTVGYGDQAFKSM GRIFASIWLLVSTLAVARAFLYLAEARVDKRHR MAKWILGQDM
Sbjct: 301 SVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDM 360

Query: 361 TVSEFLAADIDNNGFV-RSEYVVYKLKEMGKVSEKDIMQISNNFDRLDTGKCGKITLADL 420
           T+SEFLAADIDNNGFV RSEYV+YKLKEMG+VSEKD+MQISNNFDRLD+GKCGKITLADL
Sbjct: 361 TISEFLAADIDNNGFVSRSEYVIYKLKEMGRVSEKDVMQISNNFDRLDSGKCGKITLADL 420

Query: 421 LESHH 425
           LESHH
Sbjct: 421 LESHH 425

BLAST of Sgr019171 vs. ExPASy TrEMBL
Match: A0A6J1ED61 (two-pore potassium channel 3-like OS=Cucurbita moschata OX=3662 GN=LOC111433251 PE=3 SV=1)

HSP 1 Score: 764.6 bits (1973), Expect = 2.2e-217
Identity = 393/426 (92.25%), Postives = 407/426 (95.54%), Query Frame = 0

Query: 1   MDKEPLLPYLSPRGKPPPPPPQ-LCPLPEHDEITLPLTTTEFKDRLIFGPSSSPQDASPI 60
           M+KEPLLPY+SPRGKP PPPPQ LCPLPE+DEITLP+T TEFKDRLIFGPSSSPQD SPI
Sbjct: 1   MEKEPLLPYISPRGKPLPPPPQLLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDTSPI 60

Query: 61  FDALTLSLSSSRPSASSSLQDPSTLLDPKSQSQSQQAWLIDSNYSWPKTNLHRSKTAPAM 120
           FDALTLSLSSSRPSASSSLQDPST L PKSQS SQQAWL+DSNYSW K+NLHRSKTAPAM
Sbjct: 61  FDALTLSLSSSRPSASSSLQDPSTPLAPKSQSPSQQAWLVDSNYSWHKSNLHRSKTAPAM 120

Query: 121 AVINDVNHSHDPKPQFGKQSIIRQAVVLLIIYLSLGVVIYWINRDNFSAHETHPVVDALY 180
           AVINDVNHS +PKPQFGKQSIIRQAVVLLI+YLSLGV+IYWINRD FSAHETHPVVDALY
Sbjct: 121 AVINDVNHSQEPKPQFGKQSIIRQAVVLLILYLSLGVLIYWINRDKFSAHETHPVVDALY 180

Query: 181 FCIVTMCTIGYGDITPNSAATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLKNVK 240
           FCIVTMCTIGYGDITPNSAATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLL+NVK
Sbjct: 181 FCIVTMCTIGYGDITPNSAATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVK 240

Query: 241 RGAQPESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGTGVMHFIENLGWLDSFYLSV 300
           RG Q ESGKSYIIDVKKGRMRIRMKVALALGVVILCIG+G GVMHF+ENLGWLDSFYLSV
Sbjct: 241 RGVQRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGLGMGVMHFVENLGWLDSFYLSV 300

Query: 301 MSVTTVGYGDQAFKSMPGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRKMAKWILGQD 360
           MSVTTVGYGDQAFKSM GRIFASIWLLVSTLAVARAFLYLAEARVDKRHR MAKWILGQD
Sbjct: 301 MSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQD 360

Query: 361 MTVSEFLAADIDNNGFV-RSEYVVYKLKEMGKVSEKDIMQISNNFDRLDTGKCGKITLAD 420
           MTVSEFLAAD DNNG V RSEYV+YKLKEMGKVSEKD+MQISNNFDRLDTG CGKITLAD
Sbjct: 361 MTVSEFLAADTDNNGCVSRSEYVLYKLKEMGKVSEKDVMQISNNFDRLDTGNCGKITLAD 420

Query: 421 LLESHH 425
           LLESHH
Sbjct: 421 LLESHH 426

BLAST of Sgr019171 vs. ExPASy TrEMBL
Match: A0A6J1IME3 (two-pore potassium channel 3-like OS=Cucurbita maxima OX=3661 GN=LOC111478359 PE=3 SV=1)

HSP 1 Score: 761.9 bits (1966), Expect = 1.4e-216
Identity = 392/426 (92.02%), Postives = 406/426 (95.31%), Query Frame = 0

Query: 1   MDKEPLLPYLSPRGKPPPPPPQ-LCPLPEHDEITLPLTTTEFKDRLIFGPSSSPQDASPI 60
           M+KEPLLPY+SPRGKP PPPPQ LCPLPE+DEITLP+T TEFKDRLIFGPSSSPQD SPI
Sbjct: 1   MEKEPLLPYMSPRGKPLPPPPQLLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDTSPI 60

Query: 61  FDALTLSLSSSRPSASSSLQDPSTLLDPKSQSQSQQAWLIDSNYSWPKTNLHRSKTAPAM 120
           FDALTLSLSSSRPSASSSLQDPST L PKSQS SQQAWL+DSNYSW K+NLHRSKTAPAM
Sbjct: 61  FDALTLSLSSSRPSASSSLQDPSTPLAPKSQSPSQQAWLVDSNYSWHKSNLHRSKTAPAM 120

Query: 121 AVINDVNHSHDPKPQFGKQSIIRQAVVLLIIYLSLGVVIYWINRDNFSAHETHPVVDALY 180
           AVINDVNHS +PKPQFGK SIIRQAVVLLI+YLSLGV+IYWINRD FSAHETHPVVDALY
Sbjct: 121 AVINDVNHSQEPKPQFGKLSIIRQAVVLLILYLSLGVLIYWINRDKFSAHETHPVVDALY 180

Query: 181 FCIVTMCTIGYGDITPNSAATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLKNVK 240
           FCIVTMCTIGYGDITPNSAATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLL+NVK
Sbjct: 181 FCIVTMCTIGYGDITPNSAATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVK 240

Query: 241 RGAQPESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGTGVMHFIENLGWLDSFYLSV 300
           RG Q ESGKSYIIDVKKGRMRIRMKVALALGVVILCIG+G GVMHF+ENLGWLDSFYLSV
Sbjct: 241 RGVQRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGLGMGVMHFVENLGWLDSFYLSV 300

Query: 301 MSVTTVGYGDQAFKSMPGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRKMAKWILGQD 360
           MSVTTVGYGDQAFKSM GRIFASIWLLVSTLAVARAFLYLAEARVDKRHR MAKWILGQD
Sbjct: 301 MSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQD 360

Query: 361 MTVSEFLAADIDNNGFV-RSEYVVYKLKEMGKVSEKDIMQISNNFDRLDTGKCGKITLAD 420
           MTVSEFLAAD DNNG V RSEYV+YKLKEMGKVSEKD+MQISNNFDRLDTG CGKITLAD
Sbjct: 361 MTVSEFLAADTDNNGCVSRSEYVLYKLKEMGKVSEKDVMQISNNFDRLDTGNCGKITLAD 420

Query: 421 LLESHH 425
           LLESHH
Sbjct: 421 LLESHH 426

BLAST of Sgr019171 vs. ExPASy TrEMBL
Match: A0A6J1CLR2 (two-pore potassium channel 3-like OS=Momordica charantia OX=3673 GN=LOC111012441 PE=3 SV=1)

HSP 1 Score: 761.1 bits (1964), Expect = 2.4e-216
Identity = 391/425 (92.00%), Postives = 404/425 (95.06%), Query Frame = 0

Query: 1   MDKEPLLPYLSPRGKPPPPPPQLCPLPEHDEITLPLTTTEFKDRLIFGPSSSPQDASPIF 60
           M+KEPLLPY SPR K PPP P LCPLPE+DEITLPLT TEFKDRLIFGPSSSPQDASPIF
Sbjct: 1   MEKEPLLPYQSPRAKLPPPIPLLCPLPENDEITLPLTPTEFKDRLIFGPSSSPQDASPIF 60

Query: 61  DALTLSLSSSRPSASSSLQDPSTLLDPKSQSQSQQAWLIDSNYSWPKTNLHRSKTAPAMA 120
           DALTLSLSSSR S SSSL DPST LDPKSQSQSQQAWL+DSNY+WPKTNLHRSKTAPAMA
Sbjct: 61  DALTLSLSSSRSSPSSSLYDPSTPLDPKSQSQSQQAWLVDSNYAWPKTNLHRSKTAPAMA 120

Query: 121 VINDVNHSHDPKPQFGKQSIIRQAVVLLIIYLSLGVVIYWINRDNFSAHETHPVVDALYF 180
           VINDVNHS + KPQFG QSIIRQAVVLLIIYLSLGV+IYWIN DNF+AHETHPVVDALYF
Sbjct: 121 VINDVNHSQERKPQFGNQSIIRQAVVLLIIYLSLGVLIYWINLDNFTAHETHPVVDALYF 180

Query: 181 CIVTMCTIGYGDITPNSAATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLKNVKR 240
           CIVTMCTIGYGDITPNS ATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLL+NVKR
Sbjct: 181 CIVTMCTIGYGDITPNSTATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKR 240

Query: 241 GAQPESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGTGVMHFIENLGWLDSFYLSVM 300
           G Q ESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVG GVMHF+E+LGWLDSFYLSVM
Sbjct: 241 GVQRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVEDLGWLDSFYLSVM 300

Query: 301 SVTTVGYGDQAFKSMPGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRKMAKWILGQDM 360
           SVTTVGYGDQAFKS+ GRIFASIWLLVSTLAVARAFLYLAEARVDKRHR MAKWILGQDM
Sbjct: 301 SVTTVGYGDQAFKSLTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDM 360

Query: 361 TVSEFLAADIDNNGFV-RSEYVVYKLKEMGKVSEKDIMQISNNFDRLDTGKCGKITLADL 420
           TVSEFLAADIDNNGFV RSEYV+YKLKEMGKVSEKD+MQISNNFDRLDTGKCGKITLADL
Sbjct: 361 TVSEFLAADIDNNGFVSRSEYVIYKLKEMGKVSEKDVMQISNNFDRLDTGKCGKITLADL 420

Query: 421 LESHH 425
           LESHH
Sbjct: 421 LESHH 425

BLAST of Sgr019171 vs. ExPASy TrEMBL
Match: A0A6J1EM72 (two-pore potassium channel 3-like OS=Cucurbita moschata OX=3662 GN=LOC111433827 PE=3 SV=1)

HSP 1 Score: 734.2 bits (1894), Expect = 3.1e-208
Identity = 381/425 (89.65%), Postives = 398/425 (93.65%), Query Frame = 0

Query: 1   MDKEPLLPYLSPRGKPPPPPPQLCPLPEHDEITLPLTTTEFKDRLIFGPSSSPQDASPIF 60
           M+ EPLLPY+SPRGK PP  PQL PLPE+DEI LP+T TEFKDRLIFGPSSSPQDAS  F
Sbjct: 1   METEPLLPYMSPRGK-PPSLPQLYPLPENDEIILPMTPTEFKDRLIFGPSSSPQDASSAF 60

Query: 61  DALTLSLSSSRPSASSSLQDPSTLLDPKSQSQSQQAWLIDSNYSWPKTNLHRSKTAPAMA 120
           DALTLSL+S RPSASSS QDPST LDPKSQS SQQ WL+DS +S  K+NLHRSKTAPAMA
Sbjct: 61  DALTLSLASPRPSASSSRQDPSTPLDPKSQSPSQQGWLVDSKHSCHKSNLHRSKTAPAMA 120

Query: 121 VINDVNHSHDPKPQFGKQSIIRQAVVLLIIYLSLGVVIYWINRDNFSAHETHPVVDALYF 180
           VIND+NHS +PKPQFGKQSIIRQAVVLLIIYLSLGVVIYWINRDNFSA ETHPVVDALYF
Sbjct: 121 VINDINHSQEPKPQFGKQSIIRQAVVLLIIYLSLGVVIYWINRDNFSASETHPVVDALYF 180

Query: 181 CIVTMCTIGYGDITPNSAATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLKNVKR 240
           CIVTMCTIGYGDITPNSAATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLL+NVKR
Sbjct: 181 CIVTMCTIGYGDITPNSAATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKR 240

Query: 241 GAQPESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGTGVMHFIENLGWLDSFYLSVM 300
           G Q ESGKSYIIDVKKGRMRIRMKVALALGVVILCIG+G GVMHF+E+LGWLDSFYLSVM
Sbjct: 241 GVQRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGIGMGVMHFVEDLGWLDSFYLSVM 300

Query: 301 SVTTVGYGDQAFKSMPGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRKMAKWILGQDM 360
           SVTTVGYGDQAFKSM GRIFASIWLLVSTLAVARAFLYLAEARVDKRHR MAKWILGQDM
Sbjct: 301 SVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDM 360

Query: 361 TVSEFLAADIDNNGFV-RSEYVVYKLKEMGKVSEKDIMQISNNFDRLDTGKCGKITLADL 420
           TVSEFLAAD DNNG V RSEYVVYKLKEMGKVSEKD+MQISNNFDRLDTGKCGKITLADL
Sbjct: 361 TVSEFLAADTDNNGCVSRSEYVVYKLKEMGKVSEKDVMQISNNFDRLDTGKCGKITLADL 420

Query: 421 LESHH 425
           L++HH
Sbjct: 421 LQNHH 424

BLAST of Sgr019171 vs. TAIR 10
Match: AT4G18160.1 (Ca2+ activated outward rectifying K+ channel 6 )

HSP 1 Score: 503.8 bits (1296), Expect = 1.3e-142
Identity = 276/427 (64.64%), Postives = 336/427 (78.69%), Query Frame = 0

Query: 4   EPLLPY-LSPRGKPPPPPPQLCPLPEHDE--ITLPLTTTEFKDRLIFGP-SSSPQDASPI 63
           +PLL Y +SPR K   PP  L PLPE +E  I +P+T +EFK+RLIFGP S SP+D+S  
Sbjct: 7   DPLLQYMISPRLK--KPPQLLFPLPEDNEVAIPMPMTPSEFKERLIFGPFSCSPRDSSHF 66

Query: 64  FDALTLSLSSSRPSASSSLQDPSTLLDP--KSQSQSQQAWLIDSNYSWPKTN-LHRSKTA 123
            D++     SS  +A ++    S+ LDP         + WL D   S  + + LHRSKTA
Sbjct: 67  IDSMKQPSPSSSSTAVNNPFSDSSTLDPLLPPPPPQPEPWLSDQTSSHCQGHALHRSKTA 126

Query: 124 PAMAVINDVNHSHDPK-PQFGKQSIIRQAVVLLIIYLSLGVVIYWINRDNFSAHETHPVV 183
           PAMAVIND++H    K P    +S++RQA  LL++YLSLGV+IYW+NRD++  ++THPVV
Sbjct: 127 PAMAVINDLHHPIRQKDPTETSRSVVRQAFALLVVYLSLGVLIYWLNRDHYVVNQTHPVV 186

Query: 184 DALYFCIVTMCTIGYGDITPNSAATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLL 243
           D LYFCIVTMCTIGYGDITPNS  TKLFS++FVLVGFGFIDILLSGMVSYVLDLQESY+L
Sbjct: 187 DGLYFCIVTMCTIGYGDITPNSVVTKLFSIMFVLVGFGFIDILLSGMVSYVLDLQESYML 246

Query: 244 KNVKRGAQPESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGTGVMHFIENLGWLDSF 303
            + KR  +PE  +SYIIDVKKGRMRIR+KVALALGVV+LCI VG G+MHFIE +GWLDSF
Sbjct: 247 DSAKRRDEPEKRRSYIIDVKKGRMRIRLKVALALGVVVLCIAVGVGIMHFIEEIGWLDSF 306

Query: 304 YLSVMSVTTVGYGDQAFKSMPGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRKMAKWI 363
           YLSVMSVTTVGYGD+AFK++PGR+FA+IWLLVSTLAVARAFLYLAEARVDKR+R+ AK +
Sbjct: 307 YLSVMSVTTVGYGDRAFKTLPGRLFAAIWLLVSTLAVARAFLYLAEARVDKRNRERAKKV 366

Query: 364 LGQDMTVSEFLAADIDNNGFV-RSEYVVYKLKEMGKVSEKDIMQISNNFDRLDTGKCGKI 422
           L + M+VS+F AADIDNNG V ++EYV+YKLKEM K+++KDI+ IS  FD+LD    GKI
Sbjct: 367 LCETMSVSQFFAADIDNNGCVSKAEYVIYKLKEMEKITDKDILPISKQFDKLDRCSNGKI 426

BLAST of Sgr019171 vs. TAIR 10
Match: AT5G46370.1 (Ca2+ activated outward rectifying K+ channel 2 )

HSP 1 Score: 453.8 bits (1166), Expect = 1.6e-127
Identity = 249/430 (57.91%), Postives = 325/430 (75.58%), Query Frame = 0

Query: 4   EPLLPYL-SPRGKPPPPPPQLCPLPEHDEIT--LPLTTTEFKDRLIFGP-SSSPQDASPI 63
           +PL  YL +PR    PPPP L  LPE++++T  +P+T  E K+RLIFG    S +++S  
Sbjct: 12  DPLRQYLMNPRIN--PPPPSLLTLPENNDVTIPMPITPLELKNRLIFGSFVRSRKESSLP 71

Query: 64  FDALTLSLSSSRPSASSSLQDPSTLLDPKSQSQSQQAWLIDSNYSWPKTNLHRSKTAPAM 123
            DAL+ + S+S  SA++S  D + LL P ++             S P  N HRSKTAPAM
Sbjct: 72  IDALSQNPSTS-SSATTSFSDSTDLLLPLTEPNK------PVRKSKPTINFHRSKTAPAM 131

Query: 124 AVINDVNHSHDPK--PQFGKQSIIRQAVVLLIIYLSLGVVIYWINRDNFSAHETHPVVDA 183
           A IN+++H +DPK   Q   ++I+ QAV LL++YLSLGV+IYW+NRD+++  +THPVVDA
Sbjct: 132 AAINNISHPNDPKTDQQSDSKTIVNQAVALLVVYLSLGVLIYWLNRDSYNVKQTHPVVDA 191

Query: 184 LYFCIVTMCTIGYGDITPNSAATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLKN 243
           LYFCIVTMCTIGYGDITP+S  TKLFS+ FVLVGFGF+DILLSGMV+YVLDLQE+Y+L+ 
Sbjct: 192 LYFCIVTMCTIGYGDITPDSVVTKLFSIFFVLVGFGFMDILLSGMVTYVLDLQENYMLET 251

Query: 244 VKRGA----QPESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGTGVMHFIENLGWLD 303
            +  +      +  +SYIIDVKKGRMRIR+KV LALGVV+LC+G G  +MHF+E +GWLD
Sbjct: 252 ARNESLNLNDRDKVRSYIIDVKKGRMRIRLKVGLALGVVVLCLGFGVLIMHFVEKIGWLD 311

Query: 304 SFYLSVMSVTTVGYGDQAFKSMPGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRKMAK 363
           SFY SVMSVTTVGYGD+AF ++ GR+ A++WLLVSTLAVARA L+LAE+RVDKR+R+ AK
Sbjct: 312 SFYFSVMSVTTVGYGDRAFNTLAGRLLAAMWLLVSTLAVARAILFLAESRVDKRNRERAK 371

Query: 364 WILGQDMTVSEFLAADIDNNGFV-RSEYVVYKLKEMGKVSEKDIMQISNNFDRLDTGKCG 423
            +LG+ M++S+FL ADID NG V ++E+V+YKLK+M K++EKDI  I   FD+LD    G
Sbjct: 372 KVLGESMSISQFLDADIDCNGCVSKAEFVIYKLKKMDKITEKDINPIGFQFDKLDRTNSG 431

BLAST of Sgr019171 vs. TAIR 10
Match: AT4G01840.1 (Ca2+ activated outward rectifying K+ channel 5 )

HSP 1 Score: 382.1 bits (980), Expect = 5.8e-106
Identity = 225/422 (53.32%), Postives = 283/422 (67.06%), Query Frame = 0

Query: 17  PPPPPQLCPLPEHDEITLPLTTTEFKDRLIFGPSSSPQDASPIFDALTLSLSSSRPSASS 76
           P P  +L P+PE+                   PSSS   AS     +T+  S S  S   
Sbjct: 7   PQPRFRLQPIPEN-------------------PSSSSSSAS-----ITIPRSISNTSFFH 66

Query: 77  SLQDPSTLLDPKSQSQSQQAWLIDSNYSWPKTN--------LHRSKTAPAMAVINDVNHS 136
            +     LL  +   QS Q    D +    +TN        LHRS+TAPAM +I D+   
Sbjct: 67  EISQERLLLHHQDLEQSVQDDKEDQDSDSDETNRFLSQTRPLHRSRTAPAMVIIKDLRTK 126

Query: 137 --HDPKPQFGKQSIIRQAVVLLIIYLSLGVVIYWINRDNFSAHETHPVVDALYFCIVTMC 196
                KP    +SIIRQA+ LLI+YL+LGV IY  NRD++S  ETHPVVDALYFCIVTMC
Sbjct: 127 PPETKKPSPVSKSIIRQAIFLLIVYLTLGVSIYSFNRDHYSGIETHPVVDALYFCIVTMC 186

Query: 197 TIGYGDITPNSAATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLKNVKRGAQPE- 256
           TIGYGDI P +  TK+F+V+FVL GFGF+DILLSG+V+YVLDLQES +L  ++     + 
Sbjct: 187 TIGYGDIAPLTPWTKIFAVVFVLFGFGFLDILLSGVVNYVLDLQESMILTGIQTRQHHQH 246

Query: 257 ------SGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGTGVMHFIENLGWLDSFYLSV 316
                 S K YIID +KGRMRIRMKV LAL VV+LCIGVG  V+HF+E LG++DS YLSV
Sbjct: 247 HHHHRFSAKDYIIDFEKGRMRIRMKVCLALCVVVLCIGVGALVLHFVEELGFVDSVYLSV 306

Query: 317 MSVTTVGYGDQAFKSMPGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRKMAKWILGQD 376
           MSVTTVGYGD+AFK++ GR+FA++WLLVSTLAVARAFLYLAEAR+D+RHRK  K  L ++
Sbjct: 307 MSVTTVGYGDRAFKTLQGRLFAAVWLLVSTLAVARAFLYLAEARIDRRHRKAVKLALNRE 366

Query: 377 MTVSEFLAADIDNNGFV-RSEYVVYKLKEMGKVSEKDIMQISNNFDRLDTGKCGKITLAD 421
           +TV + L AD   +GF+ +SEY+V KLKEMGK+++KDI Q+   F++LD  + GKITL D
Sbjct: 367 ITVDDLLKADTYQHGFISKSEYIVLKLKEMGKITQKDIDQVVIQFEKLDPNQIGKITLPD 404

BLAST of Sgr019171 vs. TAIR 10
Match: AT5G55630.1 (Outward rectifying potassium channel protein )

HSP 1 Score: 213.4 bits (542), Expect = 3.6e-55
Identity = 124/344 (36.05%), Postives = 206/344 (59.88%), Query Frame = 0

Query: 88  KSQSQSQQAWLIDSNYSWPKTNLHRSKTAP-AMAVINDVNHSHDPKPQFGKQSI------ 147
           K  + +Q   L     S  K  L RS++AP    + ND     +P P   K  +      
Sbjct: 15  KIDTMAQDFNLNSRTSSSRKRRLRRSRSAPRGDCMYNDDVKIDEPPPHPSKIPMFSDLNP 74

Query: 148 -IRQAVVLLIIYLSLGVVIYWINRDNFSAHETHPVVDALYFCIVTMCTIGYGDITPNSAA 207
            +R+ ++ L +YL++G + +++ RD  S H+T  VVDALYFCIVTM T+GYGD+ PNS+A
Sbjct: 75  NLRRVIMFLALYLTIGTLCFYLVRDQISGHKTSGVVDALYFCIVTMTTVGYGDLVPNSSA 134

Query: 208 TKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLK--NVKRGAQPESGKSYIIDVKKG 267
           ++L +  FV  G   +  LLS    Y+++ QE+ L++  ++++   P      + ++   
Sbjct: 135 SRLLACAFVFSGMVLVGHLLSRAADYLVEKQEALLVRAFHLRQSFGP---TDILKELHTN 194

Query: 268 RMRIRMKVALALGVVILCIGVGTGVMHFIENLGWLDSFYLSVMSVTTVGYGDQAFKSMPG 327
           ++R +   A  L +V+L I VGT  +  +E +  + +FY    +VTT+GYGD++F S  G
Sbjct: 195 KLRYKC-YATCLVLVVLFI-VGTIFLVMVEKMPVISAFYCVCSTVTTLGYGDKSFNSEAG 254

Query: 328 RIFASIWLLVSTLAVARAFLYLAEARVDKRHRKMAKWILGQDMTVSEFLAADIDNNGFV- 387
           R+FA  W+L S++ +A+ FLY+AE   + + R + KW+L + +T ++  AAD+D +G V 
Sbjct: 255 RLFAVFWILTSSICLAQFFLYVAELNTENKQRALVKWVLTRRITNNDLEAADLDEDGVVG 314

Query: 388 RSEYVVYKLKEMGKVSEKDIMQISNNFDRLDTGKCGKITLADLL 421
            +E++VYKLKEMGK+ EKDI  I + F++LD  + G +T +D++
Sbjct: 315 AAEFIVYKLKEMGKIDEKDISGIMDEFEQLDYDESGTLTTSDIV 353

BLAST of Sgr019171 vs. TAIR 10
Match: AT5G55630.2 (Outward rectifying potassium channel protein )

HSP 1 Score: 213.4 bits (542), Expect = 3.6e-55
Identity = 124/344 (36.05%), Postives = 206/344 (59.88%), Query Frame = 0

Query: 88  KSQSQSQQAWLIDSNYSWPKTNLHRSKTAP-AMAVINDVNHSHDPKPQFGKQSI------ 147
           K  + +Q   L     S  K  L RS++AP    + ND     +P P   K  +      
Sbjct: 15  KIDTMAQDFNLNSRTSSSRKRRLRRSRSAPRGDCMYNDDVKIDEPPPHPSKIPMFSDLNP 74

Query: 148 -IRQAVVLLIIYLSLGVVIYWINRDNFSAHETHPVVDALYFCIVTMCTIGYGDITPNSAA 207
            +R+ ++ L +YL++G + +++ RD  S H+T  VVDALYFCIVTM T+GYGD+ PNS+A
Sbjct: 75  NLRRVIMFLALYLTIGTLCFYLVRDQISGHKTSGVVDALYFCIVTMTTVGYGDLVPNSSA 134

Query: 208 TKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLK--NVKRGAQPESGKSYIIDVKKG 267
           ++L +  FV  G   +  LLS    Y+++ QE+ L++  ++++   P      + ++   
Sbjct: 135 SRLLACAFVFSGMVLVGHLLSRAADYLVEKQEALLVRAFHLRQSFGP---TDILKELHTN 194

Query: 268 RMRIRMKVALALGVVILCIGVGTGVMHFIENLGWLDSFYLSVMSVTTVGYGDQAFKSMPG 327
           ++R +   A  L +V+L I VGT  +  +E +  + +FY    +VTT+GYGD++F S  G
Sbjct: 195 KLRYKC-YATCLVLVVLFI-VGTIFLVMVEKMPVISAFYCVCSTVTTLGYGDKSFNSEAG 254

Query: 328 RIFASIWLLVSTLAVARAFLYLAEARVDKRHRKMAKWILGQDMTVSEFLAADIDNNGFV- 387
           R+FA  W+L S++ +A+ FLY+AE   + + R + KW+L + +T ++  AAD+D +G V 
Sbjct: 255 RLFAVFWILTSSICLAQFFLYVAELNTENKQRALVKWVLTRRITNNDLEAADLDEDGVVG 314

Query: 388 RSEYVVYKLKEMGKVSEKDIMQISNNFDRLDTGKCGKITLADLL 421
            +E++VYKLKEMGK+ EKDI  I + F++LD  + G +T +D++
Sbjct: 315 AAEFIVYKLKEMGKIDEKDISGIMDEFEQLDYDESGTLTTSDIV 353