Sgr017057 (gene) Monk fruit (Qingpiguo) v1

BLAST of Sgr017057 vs. NCBI nr
Match: XP_023549991.1 (pectinesterase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 780.4 bits (2014), Expect = 9.7e-222
Identity = 407/539 (75.51%), Postives = 456/539 (84.60%), Query Frame = 0

Query: 1   MLPHLLLLSLL-VSHGRSSNNLMSCSQTPYPDLCFHFINQD------DDDQTPRITRFRT 60
           ML   LL S L ++H +S +N MSCSQTPYPDLCFHFIN +      D  Q+  ITRFR 
Sbjct: 3   MLHCFLLFSFLGITHAQSFHNFMSCSQTPYPDLCFHFINPNHNVPTTDVHQSYNITRFRN 62

Query: 61  SAIRATLNQAMATHAHLLSAMDLASFDDDDHRAKLALIDCLELYEDSINELNRSTIISSI 120
           SAIRATLNQAM  H HLLSAMDLASFDDDD R K ALIDCLELYEDSI ELNRS +++S+
Sbjct: 63  SAIRATLNQAMVAHHHLLSAMDLASFDDDD-RVKSALIDCLELYEDSITELNRS-VMNSM 122

Query: 121 NGDPFDHLTSLSASMANQQTCLNGFRDFGFSSDHLR--YFPFQILSNFSKLVSNSLAIAK 180
             +  DHLT LSAS+AN QTC +GFRDFGFSS +L    FP QILSNFSKL++NSLAI K
Sbjct: 123 RANSIDHLTLLSASLANHQTCRDGFRDFGFSSHYLNNIIFPHQILSNFSKLITNSLAITK 182

Query: 181 AAAAATSFSSSSTGGRRLLAEYSFPGWVSGDDRKLLQDTA---ADMVVAQDGTGNFRTIS 240
            AAA T+ +SS+   +RLLA  SFP WVS DDR LL+DT    ADMVVAQDG+GNFRTIS
Sbjct: 183 -AAAVTATTSSNPRSQRLLAG-SFPKWVSSDDRMLLRDTTEKRADMVVAQDGSGNFRTIS 242

Query: 241 EAVAAAAEVRKGS-GRFVIHVKGGVYRENVVIKKTMENLMMIGDGIGSTIVTAMKNVQDG 300
           EAVAAAAEVRKGS  RFVI+VKGG+YRENVVIKKT++N+MMIGDG+G TIVTAMKNV+DG
Sbjct: 243 EAVAAAAEVRKGSERRFVIYVKGGIYRENVVIKKTLKNVMMIGDGMGETIVTAMKNVKDG 302

Query: 301 STTFRSATFAVAGDGFIAKDMTFENTAGPEKHQAVALLSFADHSVFYRCSFRGYQDTLYA 360
           STTFRSATFAV G+GFIAKDMTFEN AG EKHQAV+L + AD S+FYRCSF+GYQDTLYA
Sbjct: 303 STTFRSATFAVVGEGFIAKDMTFENRAGAEKHQAVSLRTNADRSIFYRCSFKGYQDTLYA 362

Query: 361 HSKRQFYRECDIYGTVDFIFGDGAAIFQNCNIFVRKPMENQKNTVTAQARSDPNENTGFV 420
           HS R F+REC IYGT+DFIFGDGA++FQNCNIFVRKPMENQKNTVTAQ RSDPNENTGFV
Sbjct: 363 HSNRHFFRECHIYGTIDFIFGDGASVFQNCNIFVRKPMENQKNTVTAQGRSDPNENTGFV 422

Query: 421 IHNSVVRAAPDLKPVEGSYRTYLGRPWKAYSRTVIMKSFLDGLVEAAGWLPWDGDFGLST 480
           IHNSVVRAAPDLK VEG Y+TYLGRPWKAYSRTV+M+SFLDGL+E AGWLPW GDFGL T
Sbjct: 423 IHNSVVRAAPDLKAVEGVYKTYLGRPWKAYSRTVVMRSFLDGLIEPAGWLPWAGDFGLKT 482

Query: 481 VYYGEFMNAGGGASTRGRVRWPGYHVMRSAEEAGRFTVDSFLGGGGWIPATGVPFTDGL 527
           VYYGEF N+GGGAST  RV+WPGYHVM++A EA RFTV+SFL GGGWIPATGVPF +G+
Sbjct: 483 VYYGEFKNSGGGASTMRRVQWPGYHVMKTAAEAERFTVESFLEGGGWIPATGVPFANGV 537

BLAST of Sgr017057 vs. NCBI nr
Match: XP_022939088.1 (pectinesterase [Cucurbita moschata])

HSP 1 Score: 775.0 bits (2000), Expect = 4.1e-220
Identity = 405/539 (75.14%), Postives = 452/539 (83.86%), Query Frame = 0

Query: 1   MLPHLLLLSLL-VSHGRSSNNLMSCSQTPYPDLCFHFINQD------DDDQTPRITRFRT 60
           ML   LL S L ++H +S +N MSCSQTPYPDLCFHFI  +      D  Q+  ITRFR 
Sbjct: 3   MLHCFLLFSFLGITHAQSFHNFMSCSQTPYPDLCFHFIKPNHNVPTTDVHQSYHITRFRN 62

Query: 61  SAIRATLNQAMATHAHLLSAMDLASFDDDDHRAKLALIDCLELYEDSINELNRSTIISSI 120
           SAIRATLNQAM  H HLLS+MDLASF DDD R K ALIDCLELYEDSI ELNRS +++S+
Sbjct: 63  SAIRATLNQAMVAHHHLLSSMDLASFHDDD-RVKSALIDCLELYEDSITELNRS-VMNSM 122

Query: 121 NGDPFDHLTSLSASMANQQTCLNGFRDFGFSSDHLR--YFPFQILSNFSKLVSNSLAIAK 180
             +   HLT LSAS+AN QTC +GFRDFGFSS +L    FP QILSNFSKL+SNSLAI K
Sbjct: 123 KANSIGHLTLLSASLANHQTCRDGFRDFGFSSHYLNNIIFPHQILSNFSKLISNSLAITK 182

Query: 181 AAAAATSFSSSSTGGRRLLAEYSFPGWVSGDDRKLLQDTA---ADMVVAQDGTGNFRTIS 240
            AAA T+ +SS+ G +RLLA  SFP WVSGDDR LL+DT     DMVVAQDG+GNFRTIS
Sbjct: 183 -AAAVTATTSSTPGSQRLLAG-SFPKWVSGDDRMLLRDTTEKRTDMVVAQDGSGNFRTIS 242

Query: 241 EAVAAAAEVRKGS-GRFVIHVKGGVYRENVVIKKTMENLMMIGDGIGSTIVTAMKNVQDG 300
           EAVAAAAEVRKGS  RFVI+VKGG+YRENVVIKKT++N+MMIGDG+G TIVTAMKNV+DG
Sbjct: 243 EAVAAAAEVRKGSERRFVIYVKGGIYRENVVIKKTLKNVMMIGDGMGETIVTAMKNVKDG 302

Query: 301 STTFRSATFAVAGDGFIAKDMTFENTAGPEKHQAVALLSFADHSVFYRCSFRGYQDTLYA 360
           STTFRSATFAV G+GFIAKDMTFEN AG EKHQAVAL + AD S+FYRCSF+GYQDTLYA
Sbjct: 303 STTFRSATFAVVGEGFIAKDMTFENRAGAEKHQAVALRTNADRSIFYRCSFKGYQDTLYA 362

Query: 361 HSKRQFYRECDIYGTVDFIFGDGAAIFQNCNIFVRKPMENQKNTVTAQARSDPNENTGFV 420
           HS R F+REC IYGT+DFIFGDGA++FQNCNIFVRKPMENQKNTVTAQ RSDPNENTGFV
Sbjct: 363 HSNRHFFRECHIYGTIDFIFGDGASVFQNCNIFVRKPMENQKNTVTAQGRSDPNENTGFV 422

Query: 421 IHNSVVRAAPDLKPVEGSYRTYLGRPWKAYSRTVIMKSFLDGLVEAAGWLPWDGDFGLST 480
           IHNSVVRAAPDLK VEG Y+TYLGRPWKAYSRTV+M+SFLDGL+E AGWLPW GDFGL T
Sbjct: 423 IHNSVVRAAPDLKAVEGVYKTYLGRPWKAYSRTVVMRSFLDGLIEPAGWLPWAGDFGLKT 482

Query: 481 VYYGEFMNAGGGASTRGRVRWPGYHVMRSAEEAGRFTVDSFLGGGGWIPATGVPFTDGL 527
           VYYGEF N+GGGAST  RV+WPGYHVM +A EA RFTV+SFL GGGWIP TGVPF +G+
Sbjct: 483 VYYGEFKNSGGGASTMRRVQWPGYHVMTTAAEAERFTVESFLEGGGWIPGTGVPFANGV 537

BLAST of Sgr017057 vs. NCBI nr
Match: XP_022973540.1 (pectinesterase [Cucurbita maxima])

HSP 1 Score: 772.3 bits (1993), Expect = 2.7e-219
Identity = 403/538 (74.91%), Postives = 453/538 (84.20%), Query Frame = 0

Query: 1   MLPHLLLLSLL-VSHGRSSNNLMSCSQTPYPDLCFHFINQD------DDDQTPRITRFRT 60
           ML   LL S L ++H +S +  MSCSQTPYPDLCFHFIN +      D  Q+  ITRFR 
Sbjct: 1   MLRCFLLFSFLGITHAQSFHTFMSCSQTPYPDLCFHFINPNHNIPTTDVHQSYHITRFRN 60

Query: 61  SAIRATLNQAMATHAHLLSAMDLASFDDDDHRAKLALIDCLELYEDSINELNRSTIISSI 120
           SAIRATLNQAM  H HLLSAMDLASF DDD+R K ALIDCLELYEDSI ELNRS +++S+
Sbjct: 61  SAIRATLNQAMVAHHHLLSAMDLASF-DDDYRVKSALIDCLELYEDSITELNRS-MMNSM 120

Query: 121 NGDPFDHLTSLSASMANQQTCLNGFRDFGFSSDHLR-YFPFQILSNFSKLVSNSLAIAKA 180
                DHLT LSAS+AN QTC +GFRDFGFSS +L   FP QILSNFSKL+SNSLAI K 
Sbjct: 121 GAHSIDHLTLLSASLANHQTCRDGFRDFGFSSHYLNIIFPHQILSNFSKLISNSLAITK- 180

Query: 181 AAAATSFSSSSTGGRRLLAEYSFPGWVSGDDRKLLQDTA---ADMVVAQDGTGNFRTISE 240
            AA T+ +SS+   +R+LA  SFP WVSGDDR LL+DT    ADMVVAQDG+GNFRTISE
Sbjct: 181 IAAVTATTSSTPYSQRILAG-SFPKWVSGDDRMLLRDTTVKRADMVVAQDGSGNFRTISE 240

Query: 241 AVAAAAEVRKGS-GRFVIHVKGGVYRENVVIKKTMENLMMIGDGIGSTIVTAMKNVQDGS 300
           AVAAAAEVRK S  RFVI+VKGG+YRENVVIKKT++N+MMIGDG+G+TIVTAMKNV+DGS
Sbjct: 241 AVAAAAEVRKASERRFVIYVKGGIYRENVVIKKTLKNVMMIGDGMGATIVTAMKNVKDGS 300

Query: 301 TTFRSATFAVAGDGFIAKDMTFENTAGPEKHQAVALLSFADHSVFYRCSFRGYQDTLYAH 360
           TTFRSATFAV G+GFIAKDMTFEN AGPEKHQAVAL + AD S+FYRCSF+GYQDTLYAH
Sbjct: 301 TTFRSATFAVVGEGFIAKDMTFENRAGPEKHQAVALRTNADRSIFYRCSFKGYQDTLYAH 360

Query: 361 SKRQFYRECDIYGTVDFIFGDGAAIFQNCNIFVRKPMENQKNTVTAQARSDPNENTGFVI 420
           S R F+REC IYGT+DFIFGDGA++FQNCNIFVRKPMENQKNTVTAQARSDPNENTGFVI
Sbjct: 361 SNRHFFRECHIYGTIDFIFGDGASVFQNCNIFVRKPMENQKNTVTAQARSDPNENTGFVI 420

Query: 421 HNSVVRAAPDLKPVEGSYRTYLGRPWKAYSRTVIMKSFLDGLVEAAGWLPWDGDFGLSTV 480
           HNSVVRAAPDLK VEG Y+TYLGRPWKAYSRTV+M++ LDGL+E AGWLPW GDFGL TV
Sbjct: 421 HNSVVRAAPDLKAVEGVYKTYLGRPWKAYSRTVVMRNVLDGLIEPAGWLPWGGDFGLKTV 480

Query: 481 YYGEFMNAGGGASTRGRVRWPGYHVMRSAEEAGRFTVDSFLGGGGWIPATGVPFTDGL 527
           YYGEF N+GGGAST  RV+WPGYHVM++  EA RFTV+SFL GGGWIP TGVPF +G+
Sbjct: 481 YYGEFKNSGGGASTMRRVQWPGYHVMKTEAEAERFTVESFLEGGGWIPGTGVPFGNGV 534

BLAST of Sgr017057 vs. NCBI nr
Match: XP_022146317.1 (pectinesterase [Momordica charantia])

HSP 1 Score: 768.8 bits (1984), Expect = 2.9e-218
Identity = 409/540 (75.74%), Postives = 444/540 (82.22%), Query Frame = 0

Query: 1   MLPHLLLLSLLVSHGRSSNNLMSCSQTPYPDLCFHFINQDDD---------DQTPRITRF 60
           +L HLLL+SLLV HGRSS NLMSCSQTPYPDLCFHF+NQ+DD         D+T  +TRF
Sbjct: 3   ILHHLLLISLLVIHGRSSTNLMSCSQTPYPDLCFHFVNQNDDDADAISTFGDETSYVTRF 62

Query: 61  RTSAIRATLNQAMATHAHLLSAMDLASFDDDDHRAKLALIDCLELYEDSINELNRSTIIS 120
           R SAIRATLNQAM THAHLLS MD  +F   D+RAK AL+DCLELYEDSI ELNRSTI S
Sbjct: 63  RNSAIRATLNQAMTTHAHLLSNMDSDAF---DYRAKSALVDCLELYEDSIAELNRSTIDS 122

Query: 121 SINGDPFDHLTSLSASMANQQTCLNGFRDFGFSSDHLRYFPFQILSNFSKLVSNSLAIAK 180
           +      DHLT LSASMAN QTC NGFRDFG S      FPF+ILSNFSKLVSNSLAIA+
Sbjct: 123 T----TMDHLTLLSASMANHQTCENGFRDFGIS------FPFEILSNFSKLVSNSLAIAR 182

Query: 181 AAAAATSFSSSSTGGRRLLAEYSFPGWVSGDDRKLLQDT--AADMVVAQDGTGNFRTISE 240
           AAA     +++++             WV     +L+Q +  AADMVVAQDG+G+FR+ISE
Sbjct: 183 AAAGGGDNNAAASA-----------EWV-----ELVQGSAAAADMVVAQDGSGDFRSISE 242

Query: 241 AVAAAAEVRKGS---GRFVIHVKGGVYRENVVIKKTMENLMMIGDGIGSTIVTAMKNVQD 300
           AV AAAEVRKGS   GRFVI+VKGGVYRENVVIKKTM+N+MMIGDGIGSTIVTAMKNVQD
Sbjct: 243 AV-AAAEVRKGSGGGGRFVIYVKGGVYRENVVIKKTMKNVMMIGDGIGSTIVTAMKNVQD 302

Query: 301 GSTTFRSATFAVAGDGFIAKDMTFENTAGPEKHQAVALLSFADHSVFYRCSFRGYQDTLY 360
           GSTTFRS TFAV G+GFIA+DMTFEN AGPEK QAVA+LS ADHSVFYRCSFRGYQDTLY
Sbjct: 303 GSTTFRSPTFAVTGNGFIARDMTFENMAGPEKLQAVAVLSSADHSVFYRCSFRGYQDTLY 362

Query: 361 AHSKRQFYRECDIYGTVDFIFGDGAAIFQNCNIFVRKPMENQKNTVTAQARSDPNENTGF 420
           AHSKRQFYREC+IYGTVDFIFGD  A+FQNCNIFVRKPM NQKNTVTAQARSDPNENTGF
Sbjct: 363 AHSKRQFYRECEIYGTVDFIFGDATAVFQNCNIFVRKPMRNQKNTVTAQARSDPNENTGF 422

Query: 421 VIHNSVVRAAPDLKPVEGSYRTYLGRPWKAYSRTVIMKSFLDGLVEAAGWLPWDGDFGLS 480
           VIHNS V AAPDLKPVEG Y TYLGRPWKAYSRTV+M+S LDGLVE AGWLPW GDFGL 
Sbjct: 423 VIHNSAVGAAPDLKPVEGLYETYLGRPWKAYSRTVVMRSSLDGLVEPAGWLPWAGDFGLR 482

Query: 481 TVYYGEFMNAGGGASTRGRVRWPGYHVMRSAEEAGRFTVDSFLGGGGWIPATGVPFTDGL 527
           TVYYGEFMN G GAST GRVRWPGYHVM S  EAGRFTVD+FL G GWIPATGVPF +GL
Sbjct: 483 TVYYGEFMNTGDGASTMGRVRWPGYHVMTSVAEAGRFTVDNFLAGSGWIPATGVPFANGL 512

BLAST of Sgr017057 vs. NCBI nr
Match: XP_038906560.1 (pectinesterase [Benincasa hispida])

HSP 1 Score: 763.8 bits (1971), Expect = 9.4e-217
Identity = 401/538 (74.54%), Postives = 437/538 (81.23%), Query Frame = 0

Query: 1   MLPHLLLLSLL--VSHGRSSNNLMSCSQTPYPDLCFHFINQDDD------DQTPRITRFR 60
           ML  LLL SLL  + HG+S NN MSCSQTPYPDLCFH+IN +D+      D+T  ITR++
Sbjct: 3   MLHCLLLFSLLFMIIHGKSFNNFMSCSQTPYPDLCFHYINPNDNVLTADIDETSVITRYQ 62

Query: 61  TSAIRATLNQAMATHAHLLSAMDLASFDDDDHRAKLALIDCLELYEDSINELNRSTIISS 120
              IRATLNQAM  H HL  AMDL SFDDDD R K ALIDCLELYEDSI ELNR  + S 
Sbjct: 63  NLTIRATLNQAMVVHDHLRFAMDLDSFDDDD-RTKFALIDCLELYEDSITELNRLAMSSM 122

Query: 121 INGDPFDHLTSLSASMANQQTCLNGFRDFG-FSSDHLRYFPFQILSNFSKLVSNSLAIAK 180
                 DHLT LSAS+AN QTC +GFRDFG FS D     PFQILSNFSKLVSNSLAI K
Sbjct: 123 SVNSSIDHLTLLSASLANHQTCQDGFRDFGLFSFDD----PFQILSNFSKLVSNSLAITK 182

Query: 181 AAAAATSFSSSSTGGRRLLAEYSFPGWVSGDDRKLLQDTA---ADMVVAQDGTGNFRTIS 240
           AA A    SSS++GGRRLL   SFP W+   DR LLQ T    ADMVVAQDG+G+FRTIS
Sbjct: 183 AAVAVPPTSSSTSGGRRLLGG-SFPQWIYDVDRILLQGTVGNRADMVVAQDGSGDFRTIS 242

Query: 241 EAVAAAAEVRKGSGRFVIHVKGGVYRENVVIKKTMENLMMIGDGIGSTIVTAMKNVQDGS 300
           EAV AA +  KG  RFVI+VKGG+Y+ENVVIKK M+N+MMIGDG+  TIVTAMKNVQDGS
Sbjct: 243 EAVTAAEDATKGCRRFVIYVKGGIYKENVVIKKGMKNVMMIGDGMDRTIVTAMKNVQDGS 302

Query: 301 TTFRSATFAVAGDGFIAKDMTFENTAGPEKHQAVALLSFADHSVFYRCSFRGYQDTLYAH 360
           TTF+SATFAVAG+GFIAKDMTFENTAGPEKHQAVAL S AD S+FYRCSF+GYQDTLYAH
Sbjct: 303 TTFQSATFAVAGEGFIAKDMTFENTAGPEKHQAVALRSNADRSIFYRCSFKGYQDTLYAH 362

Query: 361 SKRQFYRECDIYGTVDFIFGDGAAIFQNCNIFVRKPMENQKNTVTAQARSDPNENTGFVI 420
           S RQFYREC IYGTVDFIFGD  A+FQNCNIFVRKPM NQKNT+TA ARSDPNENTGFVI
Sbjct: 363 SNRQFYRECHIYGTVDFIFGDAVAVFQNCNIFVRKPMMNQKNTITAHARSDPNENTGFVI 422

Query: 421 HNSVVRAAPDLKPVEGSYRTYLGRPWKAYSRTVIMKSFLDGLVEAAGWLPWDGDFGLSTV 480
           HNSV++AAPDLKPVEG Y TYLGRPWK YSRTVIMKSFLDGL+E AGWLPW GDFGL TV
Sbjct: 423 HNSVIQAAPDLKPVEGLYGTYLGRPWKPYSRTVIMKSFLDGLIEPAGWLPWAGDFGLRTV 482

Query: 481 YYGEFMNAGGGASTRGRVRWPGYHVMRSAEEAGRFTVDSFLGGGGWIPATGVPFTDGL 527
           YYGEFMN GGGA T+GRV+WPGYHVM SA EA RFTV+SFL GG WIPATGVPF +GL
Sbjct: 483 YYGEFMNTGGGAYTKGRVQWPGYHVMTSAAEAERFTVESFLEGGSWIPATGVPFANGL 534

BLAST of Sgr017057 vs. ExPASy Swiss-Prot
Match: O49298 (Probable pectinesterase/pectinesterase inhibitor 6 OS=Arabidopsis thaliana OX=3702 GN=PME6 PE=2 SV=1)

HSP 1 Score: 544.7 bits (1402), Expect = 1.2e-153
Identity = 298/542 (54.98%), Postives = 373/542 (68.82%), Query Frame = 0

Query: 12  VSHGRSSNNLMSCSQTPYPDLCFHFINQDD----DDQTPRITRFRTSAIRATLNQAMATH 71
           +SH  +++ + SC QTPYP +C H ++       DDQT   T F    + +T++QA+  H
Sbjct: 27  ISH-LNAHFITSCKQTPYPSVCDHHMSNSPLKTLDDQTDGFT-FHDLVVSSTMDQAVQLH 86

Query: 72  AHLLSAMDLASFDDDDHRAKLALIDCLELYEDSINELNRSTIISSINGDPFDHLTSLSAS 131
             L+S+  L         A  AL DCLELYED+I++LN S         P D  TSLSA+
Sbjct: 87  -RLVSS--LKQHHSLHKHATSALFDCLELYEDTIDQLNHSRRSYGQYSSPHDRQTSLSAA 146

Query: 132 MANQQTCLNGFRDFGFSSDHLRYFPFQILSNFSKLVSNSLAIAKAAAAA----------- 191
           +ANQ TC NGFRDF  +S + +YFP Q   N +K +SNSLA+ KAAA A           
Sbjct: 147 IANQDTCRNGFRDFKLTSSYSKYFPVQFHRNLTKSISNSLAVTKAAAEAEAVAEKYPSTG 206

Query: 192 -TSFSS--SSTGG---RRLL--AEYSFPGWVSGDDRKLLQDT----AADMVVAQDGTGNF 251
            T FS   SS GG   RRLL  ++  FP W    DRKLL+D+     AD+VVA+DG+G++
Sbjct: 207 FTKFSKQRSSAGGGSHRRLLLFSDEKFPSWFPLSDRKLLEDSKTTAKADLVVAKDGSGHY 266

Query: 252 RTISEAVAAAAEVRKGSGRFVIHVKGGVYRENVVIKKTMENLMMIGDGIGSTIVTAMKNV 311
            +I +AV AAA++ + + R VI+VK GVYRENVVIKK+++N+M+IGDGI STIVT  +NV
Sbjct: 267 TSIQQAVNAAAKLPRRNQRLVIYVKAGVYRENVVIKKSIKNVMVIGDGIDSTIVTGNRNV 326

Query: 312 QDGSTTFRSATFAVAGDGFIAKDMTFENTAGPEKHQAVALLSFADHSVFYRCSFRGYQDT 371
           QDG+TTFRSATFAV+G+GFIA+ +TFENTAGPEKHQAVAL S +D SVFY CSF+GYQDT
Sbjct: 327 QDGTTTFRSATFAVSGNGFIAQGITFENTAGPEKHQAVALRSSSDFSVFYACSFKGYQDT 386

Query: 372 LYAHSKRQFYRECDIYGTVDFIFGDGAAIFQNCNIFVRKPMENQKNTVTAQARSDPNENT 431
           LY HS RQF R C+IYGTVDFIFGD  AI QNCNI+ RKPM  QKNT+TAQ+R +P+E T
Sbjct: 387 LYLHSSRQFLRNCNIYGTVDFIFGDATAILQNCNIYARKPMSGQKNTITAQSRKEPDETT 446

Query: 432 GFVIHNSVVRAAPDLKPVEGSYRTYLGRPWKAYSRTVIMKSFLDGLVEAAGWLPWDGDFG 491
           GFVI +S V  A +         TYLGRPW+++SRTV MK  L  LV  AGWLPW G F 
Sbjct: 447 GFVIQSSTVATASE---------TYLGRPWRSHSRTVFMKCNLGALVSPAGWLPWSGSFA 506

Query: 492 LSTVYYGEFMNAGGGASTRGRVRWPGYHVMRSAEEAGRFTVDSFLGGGGWIPATGVPFTD 527
           LST+YYGE+ N G GAS  GRV+WPGYHV+++  EA +FTV++FL G  WI ATGVP  D
Sbjct: 507 LSTLYYGEYGNTGAGASVSGRVKWPGYHVIKTVTEAEKFTVENFLDGNYWITATGVPVND 554

BLAST of Sgr017057 vs. ExPASy Swiss-Prot
Match: O04887 (Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1)

HSP 1 Score: 467.6 bits (1202), Expect = 1.8e-130
Identity = 259/526 (49.24%), Postives = 335/526 (63.69%), Query Frame = 0

Query: 5   LLLLSLL-VSHGRSSNNLMS-CSQTPYPDLCFHFINQDDD-DQTPRITRFRTSAIRATLN 64
           L+L SL   S G S   + S C +TP P  C +F+ Q  D     + T F   +++  L 
Sbjct: 11  LVLFSLSHTSFGYSPEEVKSWCGKTPNPQPCEYFLTQKTDVTSIKQDTDFYKISLQLALE 70

Query: 65  QAMATHAHLLSAMDLASFDDDDHRAKLALIDCLELYEDSINELNRSTIISSINGDPFDHL 124
           +A    +   +          + R K A  DC ELYE ++ +LN+ T  SS      D  
Sbjct: 71  RATTAQSRTYTLGSKCR----NEREKAAWEDCRELYELTVLKLNQ-TSNSSPGCTKVDKQ 130

Query: 125 TSLSASMANQQTCLNGFRDFGFSSDHLRYFPFQILSNFSKLVSNSLAIAKAAAAATSFSS 184
           T LS ++ N +TC     D G       Y    + +N +KL+SN+L++ K      S+  
Sbjct: 131 TWLSTALTNLETCRASLEDLGVP----EYVLPLLSNNVTKLISNTLSLNKVPYNEPSYKD 190

Query: 185 SSTGGRRLLAEYSFPGWVSGDDRKLLQDT-AADMVVAQDGTGNFRTISEAVAAAAEVRKG 244
                        FP WV   DRKLLQ T  A++VVAQDG+GN +TI EAVAAA+  R G
Sbjct: 191 ------------GFPTWVKPGDRKLLQTTPRANIVVAQDGSGNVKTIQEAVAAAS--RAG 250

Query: 245 SGRFVIHVKGGVYRENVVIKKTMENLMMIGDGIGSTIVTAMKNVQDGSTTFRSATFAVAG 304
             R+VI++K G Y EN+ +K  ++N+M +GDGIG TI+T  K+V  G+TTF+SAT AV G
Sbjct: 251 GSRYVIYIKAGTYNENIEVK--LKNIMFVGDGIGKTIITGSKSVGGGATTFKSATVAVVG 310

Query: 305 DGFIAKDMTFENTAGPEKHQAVALLSFADHSVFYRCSFRGYQDTLYAHSKRQFYRECDIY 364
           D FIA+D+T  NTAGP  HQAVAL S +D SVFYRCSF GYQDTLY HS+RQFYRECDIY
Sbjct: 311 DNFIARDITIRNTAGPNNHQAVALRSGSDLSVFYRCSFEGYQDTLYVHSQRQFYRECDIY 370

Query: 365 GTVDFIFGDGAAIFQNCNIFVRKPMENQKNTVTAQARSDPNENTGFVIHNSVVRAAPDLK 424
           GTVDFIFG+ A + QNCNIF RKP  N+ NT+TAQ R+DPN++TG +IHN  V AA DLK
Sbjct: 371 GTVDFIFGNAAVVLQNCNIFARKP-PNRTNTLTAQGRTDPNQSTGIIIHNCRVTAASDLK 430

Query: 425 PVEGSYRTYLGRPWKAYSRTVIMKSFLDGLVEAAGWLPWDGDFGLSTVYYGEFMNAGGGA 484
           PV+ S +T+LGRPWK YSRTV +K+FLD L+  AGW+ W GDF L+T+YY E+MN G G+
Sbjct: 431 PVQSSVKTFLGRPWKQYSRTVYIKTFLDSLINPAGWMEWSGDFALNTLYYAEYMNTGPGS 490

Query: 485 STRGRVRWPGYHVMRSAEEAGRFTVDSFLGGGGWIPATGVPFTDGL 527
           ST  RV+W GYHV+ S  +  +FTV +F+ G  W+PAT VPFT GL
Sbjct: 491 STANRVKWRGYHVLTSPSQVSQFTVGNFIAGNSWLPATNVPFTSGL 510

BLAST of Sgr017057 vs. ExPASy Swiss-Prot
Match: O22149 (Probable pectinesterase/pectinesterase inhibitor 17 OS=Arabidopsis thaliana OX=3702 GN=PME17 PE=2 SV=2)

HSP 1 Score: 455.7 bits (1171), Expect = 7.2e-127
Identity = 255/530 (48.11%), Postives = 339/530 (63.96%), Query Frame = 0

Query: 4   HLLLLSLLVSHGRSSNNLMS----CSQTPYPDLCFHFINQDDDDQTPRI-TRFRTSAIRA 63
           + ++L +LV+   S  N       CSQTP P  C +F+  + +++  +  + F   +++ 
Sbjct: 10  NFVILCILVASTVSGYNQKDVKAWCSQTPNPKPCEYFLTHNSNNEPIKSESEFLKISMKL 69

Query: 64  TLNQAMATHAHLLSAMDLASFDDDDHRAKLALIDCLELYEDSINELNRSTIISSINGDPF 123
            L++A+    H  +          D R K A  DC++LY+ +++++N  T+  ++     
Sbjct: 70  VLDRAILAKTHAFTLGPKCR----DTREKAAWEDCIKLYDLTVSKIN-ETMDPNVKCSKL 129

Query: 124 DHLTSLSASMANQQTCLNGFRDFGFSSDHLRYFPFQILSNFSKLVSNSLAIAKAAAAATS 183
           D  T LS ++ N  TC  GF + G +   L      + +N S L+ N+LAI K     T 
Sbjct: 130 DAQTWLSTALTNLDTCRAGFLELGVTDIVLPL----MSNNVSNLLCNTLAINKVPFNYTP 189

Query: 184 FSSSSTGGRRLLAEYSFPGWVSGDDRKLLQDTAA--DMVVAQDGTGNFRTISEAVAAAAE 243
                           FP WV   DRKLLQ +    + VVA+DG+GNF+TI EA+ AA+ 
Sbjct: 190 PEKD-----------GFPSWVKPGDRKLLQSSTPKDNAVVAKDGSGNFKTIKEAIDAAS- 249

Query: 244 VRKGSGRFVIHVKGGVYRENVVIKKTMENLMMIGDGIGSTIVTAMKNVQDGSTTFRSATF 303
              GSGRFVI+VK GVY EN+ I+K  +N+M+ GDGIG TI+T  K+V  G+TTF SAT 
Sbjct: 250 ---GSGRFVIYVKQGVYSENLEIRK--KNVMLRGDGIGKTIITGSKSVGGGTTTFNSATV 309

Query: 304 AVAGDGFIAKDMTFENTAGPEKHQAVALLSFADHSVFYRCSFRGYQDTLYAHSKRQFYRE 363
           A  GDGFIA+ +TF NTAG    QAVAL S +D SVFY+CSF  YQDTLY HS RQFYR+
Sbjct: 310 AAVGDGFIARGITFRNTAGASNEQAVALRSGSDLSVFYQCSFEAYQDTLYVHSNRQFYRD 369

Query: 364 CDIYGTVDFIFGDGAAIFQNCNIFVRKPMENQKNTVTAQARSDPNENTGFVIHNSVVRAA 423
           CD+YGTVDFIFG+ AA+ QNCNIF R+P  ++ NT+TAQ RSDPN+NTG +IHNS V AA
Sbjct: 370 CDVYGTVDFIFGNAAAVLQNCNIFARRP-RSKTNTITAQGRSDPNQNTGIIIHNSRVTAA 429

Query: 424 PDLKPVEGSYRTYLGRPWKAYSRTVIMKSFLDGLVEAAGWLPWDGDFGLSTVYYGEFMNA 483
            DL+PV GS +TYLGRPW+ YSRTV MK+ LD L++  GWL WDG+F L T++Y EF N 
Sbjct: 430 SDLRPVLGSTKTYLGRPWRQYSRTVFMKTSLDSLIDPRGWLEWDGNFALKTLFYAEFQNT 489

Query: 484 GGGASTRGRVRWPGYHVMRSAEEAGRFTVDSFLGGGGWIPATGVPFTDGL 527
           G GAST GRV WPG+ V+ SA EA +FTV +FL GG WIP++ VPFT GL
Sbjct: 490 GPGASTSGRVTWPGFRVLGSASEASKFTVGTFLAGGSWIPSS-VPFTSGL 511

BLAST of Sgr017057 vs. ExPASy Swiss-Prot
Match: Q9FHN5 (Probable pectinesterase/pectinesterase inhibitor 59 OS=Arabidopsis thaliana OX=3702 GN=PME59 PE=2 SV=1)

HSP 1 Score: 440.3 bits (1131), Expect = 3.1e-122
Identity = 239/538 (44.42%), Postives = 338/538 (62.83%), Query Frame = 0

Query: 4   HLLLLSLLVSHGRSS----NNLMSCSQTPYPDLC-FHFINQDDDDQTPRITRFRTSAIRA 63
           HL+LL LL  H  ++    N+   C +TPYPD C  +F N +   Q  +++ FR   + A
Sbjct: 14  HLILLVLLCVHPLTTVADRNSTDWCDKTPYPDPCKCYFKNHNGFQQPTQLSEFRVMLVEA 73

Query: 64  TLNQAMATHAHLLSAMDLASFDDDDHRAKLALIDCLELYEDSINELNRSTI-ISSING-- 123
            +++A++  A L +    +  +  D + +  L DC++LY D+I +LNR+   +S   G  
Sbjct: 74  AMDRAISARAELTN----SGKNCTDSKKQAVLADCIDLYGDTIMQLNRTLHGVSPKAGAA 133

Query: 124 ---DPFDHLTSLSASMANQQTCLNGFRDFGFSSDHLRYFPFQILSN--FSKLVSNSLAIA 183
                FD  T LS ++ N +TC  G  D   +      F   I+SN   S L+SN LA+ 
Sbjct: 134 KSCTDFDAQTWLSTALTNTETCRRGSSDLNVTD-----FITPIVSNTKISHLISNCLAVN 193

Query: 184 KAAAAATSFSSSSTGGRRLLAEYSFPGWVSGDDRKLLQDTAADMVVAQDGTGNFRTISEA 243
            A   A         G     +  FP W+S  D++LL+   A++VVA+DG+G+F T+  A
Sbjct: 194 GALLTA------GNKGNTTANQKGFPTWLSRKDKRLLRAVRANLVVAKDGSGHFNTVQAA 253

Query: 244 VAAAAEVRKGSGRFVIHVKGGVYRENVVIKKTMENLMMIGDGIGSTIVTAMKNVQDGSTT 303
           +  A   +  SGRFVI+VK G+Y+EN+ ++   +++M++GDG+ STI+T  ++VQ G TT
Sbjct: 254 IDVAGRRKVTSGRFVIYVKRGIYQENINVRLNNDDIMLVGDGMRSTIITGGRSVQGGYTT 313

Query: 304 FRSATFAVAGDGFIAKDMTFENTAGPEKHQAVALLSFADHSVFYRCSFRGYQDTLYAHSK 363
           + SAT  + G  FIAK +TF NTAGP K QAVAL S +D S+FY+CS  GYQDTL  HS+
Sbjct: 314 YNSATAGIEGLHFIAKGITFRNTAGPAKGQAVALRSSSDLSIFYKCSIEGYQDTLMVHSQ 373

Query: 364 RQFYRECDIYGTVDFIFGDGAAIFQNCNIFVRKPMENQKNTVTAQARSDPNENTGFVIHN 423
           RQFYREC IYGTVDFIFG+ AA+FQNC I  R+P++ Q N +TAQ R+DP +NTG  IHN
Sbjct: 374 RQFYRECYIYGTVDFIFGNAAAVFQNCLILPRRPLKGQANVITAQGRADPFQNTGISIHN 433

Query: 424 SVVRAAPDLKPVEGSYRTYLGRPWKAYSRTVIMKSFLDGLVEAAGWLPW-DGD-FGLSTV 483
           S +  APDLKPV G+ +TY+GRPW  +SRTV+++++LD +V   GW PW +G  FGL T+
Sbjct: 434 SRILPAPDLKPVVGTVKTYMGRPWMKFSRTVVLQTYLDNVVSPVGWSPWIEGSVFGLDTL 493

Query: 484 YYGEFMNAGGGASTRGRVRWPGYHVMRSAEEAGRFTVDSFLGGGGWIPATGVPFTDGL 527
           +Y E+ N G  +STR RV W G+HV+  A +A  FTV  F+ G  W+P TG+PFT GL
Sbjct: 494 FYAEYKNTGPASSTRWRVSWKGFHVLGRASDASAFTVGKFIAGTAWLPRTGIPFTSGL 536

BLAST of Sgr017057 vs. ExPASy Swiss-Prot
Match: P83948 (Pectinesterase 3 OS=Citrus sinensis OX=2711 PE=1 SV=1)

HSP 1 Score: 437.2 bits (1123), Expect = 2.7e-121
Identity = 243/518 (46.91%), Postives = 328/518 (63.32%), Query Frame = 0

Query: 23  SCSQTPYPDLCFHFINQDDDDQTPRITRFRTSAIRATLN-QAMATHAHLLSAMDLASFDD 82
           SCS T YPDLCF  I     + + ++T  +   I  +LN    A   +      L    +
Sbjct: 72  SCSSTRYPDLCFSAI-AAVPEASKKVTS-QKDVIEMSLNITTTAVEHNYFGIQKLLKRTN 131

Query: 83  DDHRAKLALIDCLELYEDSINELNRST-------IISSINGDPFDHLTSLSASMANQQTC 142
              R K+AL DCLE  +++++EL+++           S++    D  T +SA+M NQ TC
Sbjct: 132 LTKREKVALHDCLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMTNQGTC 191

Query: 143 LNGFRDFGFSSDHLRYFPFQILSNFSKLVSNSLAIAKAAAAATSFSSSSTGGRRLLAEYS 202
           L+GF     ++ H+R        +  K+ SN+LA+ K           ++  R+L+ E S
Sbjct: 192 LDGF-SHDDANKHVRDALSDGQVHVEKMCSNALAMIKNMTDTDMMIMRTSNNRKLIEETS 251

Query: 203 ----FPGWVSGDDRKLLQDTAA--DMVVAQDGTGNFRTISEAVAAAAEVRKGSGRFVIHV 262
               +P W+S  DR+LLQ ++   ++VVA DG+GNF+T++ +VAAA +   G+ R++I +
Sbjct: 252 TVDGWPAWLSTGDRRLLQSSSVTPNVVVAADGSGNFKTVAASVAAAPQ--GGTKRYIIRI 311

Query: 263 KGGVYRENVVIKKTMENLMMIGDGIGSTIVTAMKNVQDGSTTFRSATFAVAGDGFIAKDM 322
           K GVYRENV + K  +N+M IGDG   TI+T  +NV DGSTTF+SAT AV G+GF+A+D+
Sbjct: 312 KAGVYRENVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATVAVVGEGFLARDI 371

Query: 323 TFENTAGPEKHQAVALLSFADHSVFYRCSFRGYQDTLYAHSKRQFYRECDIYGTVDFIFG 382
           TF+NTAGP KHQAVAL   AD S FY C    YQDTLY HS RQF+  C I GTVDFIFG
Sbjct: 372 TFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNCLIAGTVDFIFG 431

Query: 383 DGAAIFQNCNIFVRKPMENQKNTVTAQARSDPNENTGFVIHNSVVRAAPDLKPVEGSYRT 442
           + AA+ QNC+I  RKP   QKN VTAQ R+DPN+NTG VI  S + A  DLKPV+GS+ T
Sbjct: 432 NAAAVLQNCDIHARKPNSGQKNMVTAQGRADPNQNTGIVIQKSRIGATSDLKPVQGSFPT 491

Query: 443 YLGRPWKAYSRTVIMKSFLDGLVEAAGWLPWDGDFGLSTVYYGEFMNAGGGASTRGRVRW 502
           YLGRPWK YSRTVIM+S +  ++  AGW  WDG+F L+T++YGE  NAG GA T GRV+W
Sbjct: 492 YLGRPWKEYSRTVIMQSSITDVIHPAGWHEWDGNFALNTLFYGEHQNAGAGAGTSGRVKW 551

Query: 503 PGYHVMRSAEEAGRFTVDSFLGGGGWIPATGVPFTDGL 527
            G+ V+ SA EA  FT  SF+ G  W+ +TG PF+ GL
Sbjct: 552 KGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584

BLAST of Sgr017057 vs. ExPASy TrEMBL
Match: A0A6J1FKP5 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111445093 PE=3 SV=1)

HSP 1 Score: 775.0 bits (2000), Expect = 2.0e-220
Identity = 405/539 (75.14%), Postives = 452/539 (83.86%), Query Frame = 0

Query: 1   MLPHLLLLSLL-VSHGRSSNNLMSCSQTPYPDLCFHFINQD------DDDQTPRITRFRT 60
           ML   LL S L ++H +S +N MSCSQTPYPDLCFHFI  +      D  Q+  ITRFR 
Sbjct: 3   MLHCFLLFSFLGITHAQSFHNFMSCSQTPYPDLCFHFIKPNHNVPTTDVHQSYHITRFRN 62

Query: 61  SAIRATLNQAMATHAHLLSAMDLASFDDDDHRAKLALIDCLELYEDSINELNRSTIISSI 120
           SAIRATLNQAM  H HLLS+MDLASF DDD R K ALIDCLELYEDSI ELNRS +++S+
Sbjct: 63  SAIRATLNQAMVAHHHLLSSMDLASFHDDD-RVKSALIDCLELYEDSITELNRS-VMNSM 122

Query: 121 NGDPFDHLTSLSASMANQQTCLNGFRDFGFSSDHLR--YFPFQILSNFSKLVSNSLAIAK 180
             +   HLT LSAS+AN QTC +GFRDFGFSS +L    FP QILSNFSKL+SNSLAI K
Sbjct: 123 KANSIGHLTLLSASLANHQTCRDGFRDFGFSSHYLNNIIFPHQILSNFSKLISNSLAITK 182

Query: 181 AAAAATSFSSSSTGGRRLLAEYSFPGWVSGDDRKLLQDTA---ADMVVAQDGTGNFRTIS 240
            AAA T+ +SS+ G +RLLA  SFP WVSGDDR LL+DT     DMVVAQDG+GNFRTIS
Sbjct: 183 -AAAVTATTSSTPGSQRLLAG-SFPKWVSGDDRMLLRDTTEKRTDMVVAQDGSGNFRTIS 242

Query: 241 EAVAAAAEVRKGS-GRFVIHVKGGVYRENVVIKKTMENLMMIGDGIGSTIVTAMKNVQDG 300
           EAVAAAAEVRKGS  RFVI+VKGG+YRENVVIKKT++N+MMIGDG+G TIVTAMKNV+DG
Sbjct: 243 EAVAAAAEVRKGSERRFVIYVKGGIYRENVVIKKTLKNVMMIGDGMGETIVTAMKNVKDG 302

Query: 301 STTFRSATFAVAGDGFIAKDMTFENTAGPEKHQAVALLSFADHSVFYRCSFRGYQDTLYA 360
           STTFRSATFAV G+GFIAKDMTFEN AG EKHQAVAL + AD S+FYRCSF+GYQDTLYA
Sbjct: 303 STTFRSATFAVVGEGFIAKDMTFENRAGAEKHQAVALRTNADRSIFYRCSFKGYQDTLYA 362

Query: 361 HSKRQFYRECDIYGTVDFIFGDGAAIFQNCNIFVRKPMENQKNTVTAQARSDPNENTGFV 420
           HS R F+REC IYGT+DFIFGDGA++FQNCNIFVRKPMENQKNTVTAQ RSDPNENTGFV
Sbjct: 363 HSNRHFFRECHIYGTIDFIFGDGASVFQNCNIFVRKPMENQKNTVTAQGRSDPNENTGFV 422

Query: 421 IHNSVVRAAPDLKPVEGSYRTYLGRPWKAYSRTVIMKSFLDGLVEAAGWLPWDGDFGLST 480
           IHNSVVRAAPDLK VEG Y+TYLGRPWKAYSRTV+M+SFLDGL+E AGWLPW GDFGL T
Sbjct: 423 IHNSVVRAAPDLKAVEGVYKTYLGRPWKAYSRTVVMRSFLDGLIEPAGWLPWAGDFGLKT 482

Query: 481 VYYGEFMNAGGGASTRGRVRWPGYHVMRSAEEAGRFTVDSFLGGGGWIPATGVPFTDGL 527
           VYYGEF N+GGGAST  RV+WPGYHVM +A EA RFTV+SFL GGGWIP TGVPF +G+
Sbjct: 483 VYYGEFKNSGGGASTMRRVQWPGYHVMTTAAEAERFTVESFLEGGGWIPGTGVPFANGV 537

BLAST of Sgr017057 vs. ExPASy TrEMBL
Match: A0A6J1IBL7 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111472075 PE=3 SV=1)

HSP 1 Score: 772.3 bits (1993), Expect = 1.3e-219
Identity = 403/538 (74.91%), Postives = 453/538 (84.20%), Query Frame = 0

Query: 1   MLPHLLLLSLL-VSHGRSSNNLMSCSQTPYPDLCFHFINQD------DDDQTPRITRFRT 60
           ML   LL S L ++H +S +  MSCSQTPYPDLCFHFIN +      D  Q+  ITRFR 
Sbjct: 1   MLRCFLLFSFLGITHAQSFHTFMSCSQTPYPDLCFHFINPNHNIPTTDVHQSYHITRFRN 60

Query: 61  SAIRATLNQAMATHAHLLSAMDLASFDDDDHRAKLALIDCLELYEDSINELNRSTIISSI 120
           SAIRATLNQAM  H HLLSAMDLASF DDD+R K ALIDCLELYEDSI ELNRS +++S+
Sbjct: 61  SAIRATLNQAMVAHHHLLSAMDLASF-DDDYRVKSALIDCLELYEDSITELNRS-MMNSM 120

Query: 121 NGDPFDHLTSLSASMANQQTCLNGFRDFGFSSDHLR-YFPFQILSNFSKLVSNSLAIAKA 180
                DHLT LSAS+AN QTC +GFRDFGFSS +L   FP QILSNFSKL+SNSLAI K 
Sbjct: 121 GAHSIDHLTLLSASLANHQTCRDGFRDFGFSSHYLNIIFPHQILSNFSKLISNSLAITK- 180

Query: 181 AAAATSFSSSSTGGRRLLAEYSFPGWVSGDDRKLLQDTA---ADMVVAQDGTGNFRTISE 240
            AA T+ +SS+   +R+LA  SFP WVSGDDR LL+DT    ADMVVAQDG+GNFRTISE
Sbjct: 181 IAAVTATTSSTPYSQRILAG-SFPKWVSGDDRMLLRDTTVKRADMVVAQDGSGNFRTISE 240

Query: 241 AVAAAAEVRKGS-GRFVIHVKGGVYRENVVIKKTMENLMMIGDGIGSTIVTAMKNVQDGS 300
           AVAAAAEVRK S  RFVI+VKGG+YRENVVIKKT++N+MMIGDG+G+TIVTAMKNV+DGS
Sbjct: 241 AVAAAAEVRKASERRFVIYVKGGIYRENVVIKKTLKNVMMIGDGMGATIVTAMKNVKDGS 300

Query: 301 TTFRSATFAVAGDGFIAKDMTFENTAGPEKHQAVALLSFADHSVFYRCSFRGYQDTLYAH 360
           TTFRSATFAV G+GFIAKDMTFEN AGPEKHQAVAL + AD S+FYRCSF+GYQDTLYAH
Sbjct: 301 TTFRSATFAVVGEGFIAKDMTFENRAGPEKHQAVALRTNADRSIFYRCSFKGYQDTLYAH 360

Query: 361 SKRQFYRECDIYGTVDFIFGDGAAIFQNCNIFVRKPMENQKNTVTAQARSDPNENTGFVI 420
           S R F+REC IYGT+DFIFGDGA++FQNCNIFVRKPMENQKNTVTAQARSDPNENTGFVI
Sbjct: 361 SNRHFFRECHIYGTIDFIFGDGASVFQNCNIFVRKPMENQKNTVTAQARSDPNENTGFVI 420

Query: 421 HNSVVRAAPDLKPVEGSYRTYLGRPWKAYSRTVIMKSFLDGLVEAAGWLPWDGDFGLSTV 480
           HNSVVRAAPDLK VEG Y+TYLGRPWKAYSRTV+M++ LDGL+E AGWLPW GDFGL TV
Sbjct: 421 HNSVVRAAPDLKAVEGVYKTYLGRPWKAYSRTVVMRNVLDGLIEPAGWLPWGGDFGLKTV 480

Query: 481 YYGEFMNAGGGASTRGRVRWPGYHVMRSAEEAGRFTVDSFLGGGGWIPATGVPFTDGL 527
           YYGEF N+GGGAST  RV+WPGYHVM++  EA RFTV+SFL GGGWIP TGVPF +G+
Sbjct: 481 YYGEFKNSGGGASTMRRVQWPGYHVMKTEAEAERFTVESFLEGGGWIPGTGVPFGNGV 534

BLAST of Sgr017057 vs. ExPASy TrEMBL
Match: A0A6J1CZ97 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111015555 PE=3 SV=1)

HSP 1 Score: 768.8 bits (1984), Expect = 1.4e-218
Identity = 409/540 (75.74%), Postives = 444/540 (82.22%), Query Frame = 0

Query: 1   MLPHLLLLSLLVSHGRSSNNLMSCSQTPYPDLCFHFINQDDD---------DQTPRITRF 60
           +L HLLL+SLLV HGRSS NLMSCSQTPYPDLCFHF+NQ+DD         D+T  +TRF
Sbjct: 3   ILHHLLLISLLVIHGRSSTNLMSCSQTPYPDLCFHFVNQNDDDADAISTFGDETSYVTRF 62

Query: 61  RTSAIRATLNQAMATHAHLLSAMDLASFDDDDHRAKLALIDCLELYEDSINELNRSTIIS 120
           R SAIRATLNQAM THAHLLS MD  +F   D+RAK AL+DCLELYEDSI ELNRSTI S
Sbjct: 63  RNSAIRATLNQAMTTHAHLLSNMDSDAF---DYRAKSALVDCLELYEDSIAELNRSTIDS 122

Query: 121 SINGDPFDHLTSLSASMANQQTCLNGFRDFGFSSDHLRYFPFQILSNFSKLVSNSLAIAK 180
           +      DHLT LSASMAN QTC NGFRDFG S      FPF+ILSNFSKLVSNSLAIA+
Sbjct: 123 T----TMDHLTLLSASMANHQTCENGFRDFGIS------FPFEILSNFSKLVSNSLAIAR 182

Query: 181 AAAAATSFSSSSTGGRRLLAEYSFPGWVSGDDRKLLQDT--AADMVVAQDGTGNFRTISE 240
           AAA     +++++             WV     +L+Q +  AADMVVAQDG+G+FR+ISE
Sbjct: 183 AAAGGGDNNAAASA-----------EWV-----ELVQGSAAAADMVVAQDGSGDFRSISE 242

Query: 241 AVAAAAEVRKGS---GRFVIHVKGGVYRENVVIKKTMENLMMIGDGIGSTIVTAMKNVQD 300
           AV AAAEVRKGS   GRFVI+VKGGVYRENVVIKKTM+N+MMIGDGIGSTIVTAMKNVQD
Sbjct: 243 AV-AAAEVRKGSGGGGRFVIYVKGGVYRENVVIKKTMKNVMMIGDGIGSTIVTAMKNVQD 302

Query: 301 GSTTFRSATFAVAGDGFIAKDMTFENTAGPEKHQAVALLSFADHSVFYRCSFRGYQDTLY 360
           GSTTFRS TFAV G+GFIA+DMTFEN AGPEK QAVA+LS ADHSVFYRCSFRGYQDTLY
Sbjct: 303 GSTTFRSPTFAVTGNGFIARDMTFENMAGPEKLQAVAVLSSADHSVFYRCSFRGYQDTLY 362

Query: 361 AHSKRQFYRECDIYGTVDFIFGDGAAIFQNCNIFVRKPMENQKNTVTAQARSDPNENTGF 420
           AHSKRQFYREC+IYGTVDFIFGD  A+FQNCNIFVRKPM NQKNTVTAQARSDPNENTGF
Sbjct: 363 AHSKRQFYRECEIYGTVDFIFGDATAVFQNCNIFVRKPMRNQKNTVTAQARSDPNENTGF 422

Query: 421 VIHNSVVRAAPDLKPVEGSYRTYLGRPWKAYSRTVIMKSFLDGLVEAAGWLPWDGDFGLS 480
           VIHNS V AAPDLKPVEG Y TYLGRPWKAYSRTV+M+S LDGLVE AGWLPW GDFGL 
Sbjct: 423 VIHNSAVGAAPDLKPVEGLYETYLGRPWKAYSRTVVMRSSLDGLVEPAGWLPWAGDFGLR 482

Query: 481 TVYYGEFMNAGGGASTRGRVRWPGYHVMRSAEEAGRFTVDSFLGGGGWIPATGVPFTDGL 527
           TVYYGEFMN G GAST GRVRWPGYHVM S  EAGRFTVD+FL G GWIPATGVPF +GL
Sbjct: 483 TVYYGEFMNTGDGASTMGRVRWPGYHVMTSVAEAGRFTVDNFLAGSGWIPATGVPFANGL 512

BLAST of Sgr017057 vs. ExPASy TrEMBL
Match: A0A0A0KUU2 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G568300 PE=3 SV=1)

HSP 1 Score: 758.8 bits (1958), Expect = 1.5e-215
Identity = 395/535 (73.83%), Postives = 441/535 (82.43%), Query Frame = 0

Query: 5   LLLLSLLVSHGRSSNNLMSCSQTPYPDLCFHFINQDDD------DQTPRITRFRTSAIRA 64
           LL   L++ HGRS NN MSCSQTPYPDLCFH+IN +D+      D+T  ITRFR SAI+A
Sbjct: 8   LLFSLLVIIHGRSFNNFMSCSQTPYPDLCFHYINPNDNIRTAHIDETYLITRFRNSAIQA 67

Query: 65  TLNQAMATHAHLLSAMDLASFDDDDHRAKLALIDCLELYEDSINELNRSTIISSINGDPF 124
           TL QAM    HL S MDL SFDD+D R  +ALIDCLE YEDSI ELNRST +SS + +  
Sbjct: 68  TLYQAMKVRDHLRSTMDLGSFDDND-RNHVALIDCLEFYEDSIAELNRST-LSSTSANSI 127

Query: 125 DHLTSLSASMANQQTCLNGFRDFGFSSDHLRYF--PFQILSNFSKLVSNSLAIAKAAAAA 184
           DH T LSAS+AN QTCL+GFRDFGF  D    F  P QI+SNFSKLVSNSLAI KA AAA
Sbjct: 128 DHSTMLSASLANHQTCLDGFRDFGFLVDDSNNFFLPIQIMSNFSKLVSNSLAITKAIAAA 187

Query: 185 TSFSSSSTGGRRLLAEYSFPGWVSGDDRKLLQD---TAADMVVAQDGTGNFRTISEAVAA 244
            S SSS++ G+ LL +   P WV  DD+ LLQD     ADMVVAQDG+G+FRTISEAVAA
Sbjct: 188 PSTSSSTSCGQPLL-DGLCPEWVYDDDKTLLQDMVGKGADMVVAQDGSGDFRTISEAVAA 247

Query: 245 AAEVRK--GSGRFVIHVKGGVYRENVVIKKTMENLMMIGDGIGSTIVTAMKNVQDGSTTF 304
           A E RK  GSGRFVI+VKGG+Y+ENVVI+K M+N+MM+GDG+  TIVTAMKNVQDGSTTF
Sbjct: 248 AEEARKGSGSGRFVIYVKGGIYKENVVIEKRMKNIMMVGDGMDRTIVTAMKNVQDGSTTF 307

Query: 305 RSATFAVAGDGFIAKDMTFENTAGPEKHQAVALLSFADHSVFYRCSFRGYQDTLYAHSKR 364
           +SATFAVAG+GFIAKDMTFENTAGPEKHQAVAL S AD S+FYRCSF+GYQDTLYAHS R
Sbjct: 308 QSATFAVAGEGFIAKDMTFENTAGPEKHQAVALRSNADRSIFYRCSFKGYQDTLYAHSNR 367

Query: 365 QFYRECDIYGTVDFIFGDGAAIFQNCNIFVRKPMENQKNTVTAQARSDPNENTGFVIHNS 424
           QFYREC IYGTVDFIFGDG  +FQNCNIFVRKPM NQKNT+TAQARSDPNEN+GFVIHNS
Sbjct: 368 QFYRECHIYGTVDFIFGDGVVVFQNCNIFVRKPMLNQKNTITAQARSDPNENSGFVIHNS 427

Query: 425 VVRAAPDLKPVEGSYRTYLGRPWKAYSRTVIMKSFLDGLVEAAGWLPWDGDFGLSTVYYG 484
           V+ AAPDLKPV+G Y TYLGRPWKAYSRTVIMKS++DGL+E AGWLPW GDFGL TVYYG
Sbjct: 428 VIGAAPDLKPVQGLYGTYLGRPWKAYSRTVIMKSYMDGLIEPAGWLPWAGDFGLRTVYYG 487

Query: 485 EFMNAGGGASTRGRVRWPGYHVMRSAEEAGRFTVDSFLGGGGWIPATGVPFTDGL 527
           EFMN G GA+T+GRV WPGYHVM SA EA RFTV+SFL GGGWIPATGVPF +GL
Sbjct: 488 EFMNTGDGANTKGRVEWPGYHVMTSAVEAERFTVESFLEGGGWIPATGVPFVNGL 539

BLAST of Sgr017057 vs. ExPASy TrEMBL
Match: A0A1S3CSS2 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103504549 PE=3 SV=1)

HSP 1 Score: 753.1 bits (1943), Expect = 8.1e-214
Identity = 388/533 (72.80%), Postives = 440/533 (82.55%), Query Frame = 0

Query: 5   LLLLSLLVSHGRSSNNLMSCSQTPYPDLCFHFINQDDD------DQTPRITRFRTSAIRA 64
           LL   L++ HGRS NN MSC QTPYPDLCFH+IN +D+      D+T   TRFR SA++A
Sbjct: 8   LLFSLLVIIHGRSFNNFMSCFQTPYPDLCFHYINPNDNIPTADIDETYLNTRFRNSAVQA 67

Query: 65  TLNQAMATHAHLLSAMDLASFDDDDHRAKLALIDCLELYEDSINELNRSTIISSINGDPF 124
           TLNQA   H HL S MDL SF DDD+R K+ALIDCLE YEDSI +LNRS + SS + +  
Sbjct: 68  TLNQARKVHDHLRSIMDLGSF-DDDNRNKVALIDCLEFYEDSIAKLNRS-VFSSTSVNSI 127

Query: 125 DHLTSLSASMANQQTCLNGFRDFGFSSDHLRYFPFQILSNFSKLVSNSLAIAKAAAAATS 184
           DHLT LSAS+AN QTCL+GFRDF  S++   + P QI+SNFSKLVSNSLAI KAA A  S
Sbjct: 128 DHLTMLSASLANHQTCLDGFRDFDNSNNF--FLPIQIMSNFSKLVSNSLAITKAAVAPAS 187

Query: 185 FSSSSTGGRRLLAEYSFPGWVSGDDRKLLQD---TAADMVVAQDGTGNFRTISEAVAAAA 244
            SS+S G  + L +   P WV  DD+ LL+D     ADMVVAQDG+G+FRTISEAVAAA 
Sbjct: 188 SSSTSCG--QPLPDGLCPEWVYDDDKTLLRDMVGKGADMVVAQDGSGDFRTISEAVAAAE 247

Query: 245 EVRKGSG--RFVIHVKGGVYRENVVIKKTMENLMMIGDGIGSTIVTAMKNVQDGSTTFRS 304
           E RKGSG  RFVI+VKGG+Y+ENVVIKK+M+N+MMIGDG+  TI+TAMKNVQDGSTTF+S
Sbjct: 248 EARKGSGSERFVIYVKGGIYKENVVIKKSMKNVMMIGDGMDRTIITAMKNVQDGSTTFQS 307

Query: 305 ATFAVAGDGFIAKDMTFENTAGPEKHQAVALLSFADHSVFYRCSFRGYQDTLYAHSKRQF 364
           ATFAVAG+GFIAKDMTFENTAGPEKHQAVAL S AD S+FYRCSF+GYQDTLYAHS RQF
Sbjct: 308 ATFAVAGEGFIAKDMTFENTAGPEKHQAVALRSNADRSIFYRCSFKGYQDTLYAHSNRQF 367

Query: 365 YRECDIYGTVDFIFGDGAAIFQNCNIFVRKPMENQKNTVTAQARSDPNENTGFVIHNSVV 424
           YREC IYGTVDFIFGDGA +FQNCNIFVRKPM NQKNT+TAQ RSDPNENTGFVIHNSV+
Sbjct: 368 YRECHIYGTVDFIFGDGAVVFQNCNIFVRKPMMNQKNTITAQGRSDPNENTGFVIHNSVI 427

Query: 425 RAAPDLKPVEGSYRTYLGRPWKAYSRTVIMKSFLDGLVEAAGWLPWDGDFGLSTVYYGEF 484
           +AAPDLKPV+G Y TYLGRPWKAYSRTVIMKS+LDGL+E AGWLPW GDFGL T+YYGEF
Sbjct: 428 KAAPDLKPVQGLYGTYLGRPWKAYSRTVIMKSYLDGLIEPAGWLPWAGDFGLRTIYYGEF 487

Query: 485 MNAGGGASTRGRVRWPGYHVMRSAEEAGRFTVDSFLGGGGWIPATGVPFTDGL 527
           MN GGGA+T+GRVRWPGYHVM SA EA  FTV+SFLGG GWIP TGVPF +G+
Sbjct: 488 MNIGGGANTKGRVRWPGYHVMTSATEAEGFTVESFLGGRGWIPTTGVPFVNGV 534

BLAST of Sgr017057 vs. TAIR 10
Match: AT1G23200.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 544.7 bits (1402), Expect = 8.4e-155
Identity = 298/542 (54.98%), Postives = 373/542 (68.82%), Query Frame = 0

Query: 12  VSHGRSSNNLMSCSQTPYPDLCFHFINQDD----DDQTPRITRFRTSAIRATLNQAMATH 71
           +SH  +++ + SC QTPYP +C H ++       DDQT   T F    + +T++QA+  H
Sbjct: 27  ISH-LNAHFITSCKQTPYPSVCDHHMSNSPLKTLDDQTDGFT-FHDLVVSSTMDQAVQLH 86

Query: 72  AHLLSAMDLASFDDDDHRAKLALIDCLELYEDSINELNRSTIISSINGDPFDHLTSLSAS 131
             L+S+  L         A  AL DCLELYED+I++LN S         P D  TSLSA+
Sbjct: 87  -RLVSS--LKQHHSLHKHATSALFDCLELYEDTIDQLNHSRRSYGQYSSPHDRQTSLSAA 146

Query: 132 MANQQTCLNGFRDFGFSSDHLRYFPFQILSNFSKLVSNSLAIAKAAAAA----------- 191
           +ANQ TC NGFRDF  +S + +YFP Q   N +K +SNSLA+ KAAA A           
Sbjct: 147 IANQDTCRNGFRDFKLTSSYSKYFPVQFHRNLTKSISNSLAVTKAAAEAEAVAEKYPSTG 206

Query: 192 -TSFSS--SSTGG---RRLL--AEYSFPGWVSGDDRKLLQDT----AADMVVAQDGTGNF 251
            T FS   SS GG   RRLL  ++  FP W    DRKLL+D+     AD+VVA+DG+G++
Sbjct: 207 FTKFSKQRSSAGGGSHRRLLLFSDEKFPSWFPLSDRKLLEDSKTTAKADLVVAKDGSGHY 266

Query: 252 RTISEAVAAAAEVRKGSGRFVIHVKGGVYRENVVIKKTMENLMMIGDGIGSTIVTAMKNV 311
            +I +AV AAA++ + + R VI+VK GVYRENVVIKK+++N+M+IGDGI STIVT  +NV
Sbjct: 267 TSIQQAVNAAAKLPRRNQRLVIYVKAGVYRENVVIKKSIKNVMVIGDGIDSTIVTGNRNV 326

Query: 312 QDGSTTFRSATFAVAGDGFIAKDMTFENTAGPEKHQAVALLSFADHSVFYRCSFRGYQDT 371
           QDG+TTFRSATFAV+G+GFIA+ +TFENTAGPEKHQAVAL S +D SVFY CSF+GYQDT
Sbjct: 327 QDGTTTFRSATFAVSGNGFIAQGITFENTAGPEKHQAVALRSSSDFSVFYACSFKGYQDT 386

Query: 372 LYAHSKRQFYRECDIYGTVDFIFGDGAAIFQNCNIFVRKPMENQKNTVTAQARSDPNENT 431
           LY HS RQF R C+IYGTVDFIFGD  AI QNCNI+ RKPM  QKNT+TAQ+R +P+E T
Sbjct: 387 LYLHSSRQFLRNCNIYGTVDFIFGDATAILQNCNIYARKPMSGQKNTITAQSRKEPDETT 446

Query: 432 GFVIHNSVVRAAPDLKPVEGSYRTYLGRPWKAYSRTVIMKSFLDGLVEAAGWLPWDGDFG 491
           GFVI +S V  A +         TYLGRPW+++SRTV MK  L  LV  AGWLPW G F 
Sbjct: 447 GFVIQSSTVATASE---------TYLGRPWRSHSRTVFMKCNLGALVSPAGWLPWSGSFA 506

Query: 492 LSTVYYGEFMNAGGGASTRGRVRWPGYHVMRSAEEAGRFTVDSFLGGGGWIPATGVPFTD 527
           LST+YYGE+ N G GAS  GRV+WPGYHV+++  EA +FTV++FL G  WI ATGVP  D
Sbjct: 507 LSTLYYGEYGNTGAGASVSGRVKWPGYHVIKTVTEAEKFTVENFLDGNYWITATGVPVND 554

BLAST of Sgr017057 vs. TAIR 10
Match: AT2G45220.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 455.7 bits (1171), Expect = 5.1e-128
Identity = 255/530 (48.11%), Postives = 339/530 (63.96%), Query Frame = 0

Query: 4   HLLLLSLLVSHGRSSNNLMS----CSQTPYPDLCFHFINQDDDDQTPRI-TRFRTSAIRA 63
           + ++L +LV+   S  N       CSQTP P  C +F+  + +++  +  + F   +++ 
Sbjct: 10  NFVILCILVASTVSGYNQKDVKAWCSQTPNPKPCEYFLTHNSNNEPIKSESEFLKISMKL 69

Query: 64  TLNQAMATHAHLLSAMDLASFDDDDHRAKLALIDCLELYEDSINELNRSTIISSINGDPF 123
            L++A+    H  +          D R K A  DC++LY+ +++++N  T+  ++     
Sbjct: 70  VLDRAILAKTHAFTLGPKCR----DTREKAAWEDCIKLYDLTVSKIN-ETMDPNVKCSKL 129

Query: 124 DHLTSLSASMANQQTCLNGFRDFGFSSDHLRYFPFQILSNFSKLVSNSLAIAKAAAAATS 183
           D  T LS ++ N  TC  GF + G +   L      + +N S L+ N+LAI K     T 
Sbjct: 130 DAQTWLSTALTNLDTCRAGFLELGVTDIVLPL----MSNNVSNLLCNTLAINKVPFNYTP 189

Query: 184 FSSSSTGGRRLLAEYSFPGWVSGDDRKLLQDTAA--DMVVAQDGTGNFRTISEAVAAAAE 243
                           FP WV   DRKLLQ +    + VVA+DG+GNF+TI EA+ AA+ 
Sbjct: 190 PEKD-----------GFPSWVKPGDRKLLQSSTPKDNAVVAKDGSGNFKTIKEAIDAAS- 249

Query: 244 VRKGSGRFVIHVKGGVYRENVVIKKTMENLMMIGDGIGSTIVTAMKNVQDGSTTFRSATF 303
              GSGRFVI+VK GVY EN+ I+K  +N+M+ GDGIG TI+T  K+V  G+TTF SAT 
Sbjct: 250 ---GSGRFVIYVKQGVYSENLEIRK--KNVMLRGDGIGKTIITGSKSVGGGTTTFNSATV 309

Query: 304 AVAGDGFIAKDMTFENTAGPEKHQAVALLSFADHSVFYRCSFRGYQDTLYAHSKRQFYRE 363
           A  GDGFIA+ +TF NTAG    QAVAL S +D SVFY+CSF  YQDTLY HS RQFYR+
Sbjct: 310 AAVGDGFIARGITFRNTAGASNEQAVALRSGSDLSVFYQCSFEAYQDTLYVHSNRQFYRD 369

Query: 364 CDIYGTVDFIFGDGAAIFQNCNIFVRKPMENQKNTVTAQARSDPNENTGFVIHNSVVRAA 423
           CD+YGTVDFIFG+ AA+ QNCNIF R+P  ++ NT+TAQ RSDPN+NTG +IHNS V AA
Sbjct: 370 CDVYGTVDFIFGNAAAVLQNCNIFARRP-RSKTNTITAQGRSDPNQNTGIIIHNSRVTAA 429

Query: 424 PDLKPVEGSYRTYLGRPWKAYSRTVIMKSFLDGLVEAAGWLPWDGDFGLSTVYYGEFMNA 483
            DL+PV GS +TYLGRPW+ YSRTV MK+ LD L++  GWL WDG+F L T++Y EF N 
Sbjct: 430 SDLRPVLGSTKTYLGRPWRQYSRTVFMKTSLDSLIDPRGWLEWDGNFALKTLFYAEFQNT 489

Query: 484 GGGASTRGRVRWPGYHVMRSAEEAGRFTVDSFLGGGGWIPATGVPFTDGL 527
           G GAST GRV WPG+ V+ SA EA +FTV +FL GG WIP++ VPFT GL
Sbjct: 490 GPGASTSGRVTWPGFRVLGSASEASKFTVGTFLAGGSWIPSS-VPFTSGL 511

BLAST of Sgr017057 vs. TAIR 10
Match: AT5G51490.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 440.3 bits (1131), Expect = 2.2e-123
Identity = 239/538 (44.42%), Postives = 338/538 (62.83%), Query Frame = 0

Query: 4   HLLLLSLLVSHGRSS----NNLMSCSQTPYPDLC-FHFINQDDDDQTPRITRFRTSAIRA 63
           HL+LL LL  H  ++    N+   C +TPYPD C  +F N +   Q  +++ FR   + A
Sbjct: 14  HLILLVLLCVHPLTTVADRNSTDWCDKTPYPDPCKCYFKNHNGFQQPTQLSEFRVMLVEA 73

Query: 64  TLNQAMATHAHLLSAMDLASFDDDDHRAKLALIDCLELYEDSINELNRSTI-ISSING-- 123
            +++A++  A L +    +  +  D + +  L DC++LY D+I +LNR+   +S   G  
Sbjct: 74  AMDRAISARAELTN----SGKNCTDSKKQAVLADCIDLYGDTIMQLNRTLHGVSPKAGAA 133

Query: 124 ---DPFDHLTSLSASMANQQTCLNGFRDFGFSSDHLRYFPFQILSN--FSKLVSNSLAIA 183
                FD  T LS ++ N +TC  G  D   +      F   I+SN   S L+SN LA+ 
Sbjct: 134 KSCTDFDAQTWLSTALTNTETCRRGSSDLNVTD-----FITPIVSNTKISHLISNCLAVN 193

Query: 184 KAAAAATSFSSSSTGGRRLLAEYSFPGWVSGDDRKLLQDTAADMVVAQDGTGNFRTISEA 243
            A   A         G     +  FP W+S  D++LL+   A++VVA+DG+G+F T+  A
Sbjct: 194 GALLTA------GNKGNTTANQKGFPTWLSRKDKRLLRAVRANLVVAKDGSGHFNTVQAA 253

Query: 244 VAAAAEVRKGSGRFVIHVKGGVYRENVVIKKTMENLMMIGDGIGSTIVTAMKNVQDGSTT 303
           +  A   +  SGRFVI+VK G+Y+EN+ ++   +++M++GDG+ STI+T  ++VQ G TT
Sbjct: 254 IDVAGRRKVTSGRFVIYVKRGIYQENINVRLNNDDIMLVGDGMRSTIITGGRSVQGGYTT 313

Query: 304 FRSATFAVAGDGFIAKDMTFENTAGPEKHQAVALLSFADHSVFYRCSFRGYQDTLYAHSK 363
           + SAT  + G  FIAK +TF NTAGP K QAVAL S +D S+FY+CS  GYQDTL  HS+
Sbjct: 314 YNSATAGIEGLHFIAKGITFRNTAGPAKGQAVALRSSSDLSIFYKCSIEGYQDTLMVHSQ 373

Query: 364 RQFYRECDIYGTVDFIFGDGAAIFQNCNIFVRKPMENQKNTVTAQARSDPNENTGFVIHN 423
           RQFYREC IYGTVDFIFG+ AA+FQNC I  R+P++ Q N +TAQ R+DP +NTG  IHN
Sbjct: 374 RQFYRECYIYGTVDFIFGNAAAVFQNCLILPRRPLKGQANVITAQGRADPFQNTGISIHN 433

Query: 424 SVVRAAPDLKPVEGSYRTYLGRPWKAYSRTVIMKSFLDGLVEAAGWLPW-DGD-FGLSTV 483
           S +  APDLKPV G+ +TY+GRPW  +SRTV+++++LD +V   GW PW +G  FGL T+
Sbjct: 434 SRILPAPDLKPVVGTVKTYMGRPWMKFSRTVVLQTYLDNVVSPVGWSPWIEGSVFGLDTL 493

Query: 484 YYGEFMNAGGGASTRGRVRWPGYHVMRSAEEAGRFTVDSFLGGGGWIPATGVPFTDGL 527
           +Y E+ N G  +STR RV W G+HV+  A +A  FTV  F+ G  W+P TG+PFT GL
Sbjct: 494 FYAEYKNTGPASSTRWRVSWKGFHVLGRASDASAFTVGKFIAGTAWLPRTGIPFTSGL 536

BLAST of Sgr017057 vs. TAIR 10
Match: AT3G49220.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 430.3 bits (1105), Expect = 2.3e-120
Identity = 240/517 (46.42%), Postives = 318/517 (61.51%), Query Frame = 0

Query: 23  SCSQTPYPDLCFHFINQDDDDQTPRITRFRTSAIRATLNQAMATHAHLLSAMDLASFDDD 82
           +C  T +P+LC   ++   D             I  T+N  +   +H L +    SF D 
Sbjct: 90  ACELTRFPELC---VDSLMDFPGSLAASSSKDLIHVTVNMTLHHFSHALYSSASLSFVDM 149

Query: 83  DHRAKLALIDCLELYEDSINELNR--STIISSINGDPFDHLTSLSASMANQQTCLNGFR- 142
             RA+ A   C+EL +DS++ L+R  S+++SS +  P D  T LSA++ N  TC  GF  
Sbjct: 150 PPRARSAYDSCVELLDDSVDALSRALSSVVSS-SAKPQDVTTWLSAALTNHDTCTEGFDG 209

Query: 143 -DFGFSSDHLRYFPFQILSNFSKLVSNSLAIAKAAAAATSFSSSSTGGRRLLA----EYS 202
            D G   DH+       L N S+LVSN LAI  A+     F+      RRLL     E  
Sbjct: 210 VDDGGVKDHMT----AALQNLSELVSNCLAIFSASHDGDDFAGVPIQNRRLLGVEEREEK 269

Query: 203 FPGWVSGDDRKLLQ----DTAADMVVAQDGTGNFRTISEAVAAAAEVRKGSGRFVIHVKG 262
           FP W+   +R++L+       AD++V++DG G  +TISEA+  A +    + R +I+VK 
Sbjct: 270 FPRWMRPKEREILEMPVSQIQADIIVSKDGNGTCKTISEAIKKAPQ--NSTRRIIIYVKA 329

Query: 263 GVYRE-NVVIKKTMENLMMIGDGIGSTIVTAMKNVQDGSTTFRSATFAVAGDGFIAKDMT 322
           G Y E N+ + +   NLM +GDG G T+++  K++ D  TTF +A+FA  G GFIA+D+T
Sbjct: 330 GRYEENNLKVGRKKINLMFVGDGKGKTVISGGKSIFDNITTFHTASFAATGAGFIARDIT 389

Query: 323 FENTAGPEKHQAVALLSFADHSVFYRCSFRGYQDTLYAHSKRQFYRECDIYGTVDFIFGD 382
           FEN AGP KHQAVAL   ADH+V YRC+  GYQDTLY HS RQF+RECDIYGTVDFIFG+
Sbjct: 390 FENWAGPAKHQAVALRIGADHAVIYRCNIIGYQDTLYVHSNRQFFRECDIYGTVDFIFGN 449

Query: 383 GAAIFQNCNIFVRKPMENQKNTVTAQARSDPNENTGFVIHNSVVRAAPDLKPVEGSYRTY 442
            A + QNC+I+ RKPM+ QKNT+TAQ R DPN+NTG  IH S V AA DL+   GS +TY
Sbjct: 450 AAVVLQNCSIYARKPMDFQKNTITAQNRKDPNQNTGISIHASRVLAASDLQATNGSTQTY 509

Query: 443 LGRPWKAYSRTVIMKSFLDGLVEAAGWLPWDGDFGLSTVYYGEFMNAGGGASTRGRVRWP 502
           LGRPWK +SRTV M S++ G V   GWL W+  F L T+YYGE++N+G G+    RV WP
Sbjct: 510 LGRPWKLFSRTVYMMSYIGGHVHTRGWLEWNTTFALDTLYYGEYLNSGPGSGLGQRVSWP 569

Query: 503 GYHVMRSAEEAGRFTVDSFLGGGGWIPATGVPFTDGL 527
           GY V+ S  EA RFTV  F+ G  W+P+TGV F  GL
Sbjct: 570 GYRVINSTAEANRFTVAEFIYGSSWLPSTGVSFLAGL 596

BLAST of Sgr017057 vs. TAIR 10
Match: AT5G51500.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 424.1 bits (1089), Expect = 1.7e-118
Identity = 238/541 (43.99%), Postives = 336/541 (62.11%), Query Frame = 0

Query: 4   HLLLLSLL-------VSHGRSSNNLMSCSQTPYPDLCFHFINQDDDDQTP-RITRFRTSA 63
           HLLL+ LL       V+ G S+N    C +TPYP  C  +  +    + P +I+ FR   
Sbjct: 15  HLLLILLLCLRPLTTVADGNSTNIDGWCDKTPYPYPCKRYFIKHSGFRLPTQISEFRVLL 74

Query: 64  IRATLNQAMATHAHLLSAMDLASFDDDDHRAKLALIDCLELYEDSINELNRSTI-ISSIN 123
           + A +++A++    L +    +S +  D + +  L DC+ LY D++ +LNR+   +SS  
Sbjct: 75  VEAAMDRAVSAWDKLTN----SSKNCTDFKKQAVLADCINLYGDTVMQLNRTLQGVSSKT 134

Query: 124 G---DPFDHLTSLSASMANQQTCLNGFRDFGFSSDHLRYFPFQILSN--FSKLVSNSLAI 183
           G     FD  T LS ++ N +TC  G  D   S      F   I+SN   S L+SN LA+
Sbjct: 135 GRRCTDFDAQTWLSTALTNTETCRRGSSDLNVSD-----FTTPIVSNTKISHLISNCLAV 194

Query: 184 AKAAAAATSFSSSSTGGRRLLAEYSFPGWVSGDDRKL--LQDTAADMVVAQDGTGNFRTI 243
             A   A   + S+TG  +      FP WVS  +R+L  LQ   A++VVA+DG+G+F+T+
Sbjct: 195 NGALLTAGK-NDSTTGDSK-----GFPTWVSRKERRLLQLQSVRANLVVAKDGSGHFKTV 254

Query: 244 SEAVAAAAEVRKGSGRFVIHVKGGVYRENVVIKKTMENLMMIGDGIGSTIVTAMKNVQDG 303
             A+  A   +  SGRFVI+VK G+Y+EN+ ++   +N+M++GDG+  TI+T  ++V+ G
Sbjct: 255 QAAIDVAGRRKVTSGRFVIYVKRGIYQENLNVRLNNDNIMLVGDGMRYTIITGGRSVKGG 314

Query: 304 STTFRSATFAVAGDGFIAKDMTFENTAGPEKHQAVALLSFADHSVFYRCSFRGYQDTLYA 363
            TT+ SAT  + G  FIAK + F+NTAGP K QAVAL S +D S+FYRCS  GYQDTL  
Sbjct: 315 YTTYSSATAGIEGLHFIAKGIAFQNTAGPAKGQAVALRSSSDLSIFYRCSIEGYQDTLMV 374

Query: 364 HSKRQFYRECDIYGTVDFIFGDGAAIFQNCNIFVRKPMENQKNTVTAQARSDPNENTGFV 423
           HS+RQFYREC IYGTVDFIFG+ A +FQNC I  R P++ Q N +TAQ R+D  +NTG  
Sbjct: 375 HSQRQFYRECYIYGTVDFIFGNAAVVFQNCIILPRLPLKGQANVITAQGRTDLFQNTGIS 434

Query: 424 IHNSVVRAAPDLKPVEGSYRTYLGRPWKAYSRTVIMKSFLDGLVEAAGWLPW--DGDFGL 483
           IHNS++  APDLKPV  S +TY+GRPW  YSRTV++K+++D +V   GW PW     +GL
Sbjct: 435 IHNSIIIPAPDLKPVVRSVKTYMGRPWMMYSRTVVLKTYIDSVVSPVGWSPWTKGSTYGL 494

Query: 484 STVYYGEFMNAGGGASTRGRVRWPGYHVMRSAEEAGRFTVDSFLGGGGWIPATGVPFTDG 527
            T++Y E+ N G  +STR RVRW G+HV+  A +A  F+V  F+ G  W+P +G+PFT  
Sbjct: 495 DTLFYAEYKNIGPASSTRWRVRWKGFHVLSKASDASAFSVGKFIAGTAWLPGSGIPFTSE 540