Sgr015334 (gene) Monk fruit (Qingpiguo) v1

Overview


Name	Sgr015334
Type	gene
Organism	Siraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Description	O-methyltransferase family protein
Location	tig00003412: 864464 .. 866651 (-)
RNA-Seq Expression	Sgr015334
Synteny	Sgr015334

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATTTGGAGGGTGGAAATGTCGCAGTTGTTGCCGGCGACGAGCTCCTGGAAGCTCAAGCTCACATATGGAACCACATCTTCAACTTCATCAACTCCATGGCCCTCAAATGCGCAGTCCAGCTCGGCATACCAGACGCCATCCACCGCCATGGCTGCCACCCCATGCCCCTCTCTGAGCTTGTTTCAGCCCTCCTACTCAATCCCCGAAAGACCCATGACGTATACCGCTTAATGCGTATACTAACCCACTCGGGTTTCTTTACAATACAGAAAGTGCGCAAAAGTGGTGAACAAGAAGAAGAAGAAGAAGAAGAAGGATATGTGCTGACCAATTCATCTCGTCTTCTCCTTAAAGACAACCCCTCTGCTGTATCTCCTTTCTTGCTTTCCATGCTCGACCCAGCCCTCGTGGAGCCATGGCAGTTCCTCTCTACCTGGTTCCAAAGTGACGATCGAACCCCATTTGATACGGCTCATGGAATGCCGTTCTGGGAGTATATGGGCAATAAGCAGAAAGATGGCGAGGTTTTTAACGCAGCCATGGCCAGCGATGCAAGGTTGGTGACGAGTATTGTGTTGGGCAAATATAAAGGCGTGTTTGACGGAGTTGAGTCGCTGGTTGATGTGGGAGGTGGCACTGGAACTGTGGCGAGAGCCATTGCTGAGGCTTTTCCAGAAATAGAATGTACTGTGTTTGATCTTCCCCAAGTTGTGGCTGACTTGAAAGGGAAAGACAACTTGAAATATGTTGAAGGGGACATGTTTGAAGCTATTCCTTCTGCTGATGCACTCCTGTTGAAGGTAAAACTAATTTTTCTTGTTGGTATATGCGTTTCAAGAGCTCACATATTAAAATATTTTTGCTATCAATACTTATTAATGACATAAATGAATAATTTCTCTGGTCATAATTAGATATGGTCGTCAAAATATCATTATTGATTTATCAACTATGCATCGAAGGGTGTATATATTGTTTAATTTGATTACACGTTTTCGTGAATAGAAAATTGAACTGTGCTATTCGATTTGCTATATGTGCTAATTAGATGATTTTTTCTTTTTGTACAAATGGATATTTTGGTTTGTTTTAAATCCATTCGATTTTTCAATGTTGGGTGTATTATGCATCATTCAAATATTTTAAATTTTAAGCCCAATTTTGAGTTTGATTTCAGTCTTTTTTGAAAAAAAAAAAAAAACTTTCCTATCTTTTTTCTTTTTCATTTTCATTTTAAGTTTCTCAATGAAATTCAATTTTTTATTTATTTATTTTCGATAACGAATAAACCCAATTTAAAAAAAGTTTCAGTTTTCACACATTGTTTTATAGCCAATATAACCCAAAATAATTATTAGAAAAATCAAATTGAATAGGTTGGATTGGTTCAATTCAATTTGACATTTTTTTCAATTTATATATAGCCCTACATATATCTAATAGTTATAAGAGTGATAATCACATTATGAAAATCACATAAAACGCATTTTTCAATTTTTCAACCTTACATTTAATGTTTATGAAAGAAATAATCCATTTATATATCATCTACACTTTAAACAATATTTTCCCCCTACATTAGCCTATTTACAACCATAGGTTGAACCTTTAATGGGGAAGCATAAATAAAATAAGCTAAATGGATTAAACAAAGGGTTCAAGGAAAAGAAATATTTAAGGCAAAGTTGCACCAGCTAGAAAAATTCCCCCAATGCATATATGCAACTGTTATATTATGATGAGATTATTATAATTTTTTTAACCTTCCCAAAGGATTTTTTTTAGTTCAATGAGAGGTGAGAGTGGGAATTGGAACCTCTGACCTTTAGGAAGTTATTGAATGCCTTAATCAATTGAGCTATGCTCGATTTGGCACCTTCCCAAATTAAATATTTGATATATGCTTAATAAAGTTTAATATTGTGAATTGTGAAATATGATATTTGCACATGTGACAAAACAGTGGATATTACATGATTGGAGTGATGAAGAATGTGTGAAGATACTGAAGAAATGCAAAGAGGCAATTACAGGCAATGAGAAAAAAGGGAAGGTGATTGTAATAGATATGGTGGTAGGCAATAAAAAAAGGACGACTGGATTGAGACTCAACTATTCTTTGACATGTTGATGATGATTTGGTAGGTGGAAAAGAGAGAGATGAAAAAGAGTGGGCTCAACTGA

mRNA sequence

ATGGATTTGGAGGGTGGAAATGTCGCAGTTGTTGCCGGCGACGAGCTCCTGGAAGCTCAAGCTCACATATGGAACCACATCTTCAACTTCATCAACTCCATGGCCCTCAAATGCGCAGTCCAGCTCGGCATACCAGACGCCATCCACCGCCATGGCTGCCACCCCATGCCCCTCTCTGAGCTTGTTTCAGCCCTCCTACTCAATCCCCGAAAGACCCATGACGTATACCGCTTAATGCGTATACTAACCCACTCGGGTTTCTTTACAATACAGAAAGTGCGCAAAAGTGGTGAACAAGAAGAAGAAGAAGAAGAAGAAGGATATGTGCTGACCAATTCATCTCGTCTTCTCCTTAAAGACAACCCCTCTGCTGTATCTCCTTTCTTGCTTTCCATGCTCGACCCAGCCCTCGTGGAGCCATGGCAGTTCCTCTCTACCTGGTTCCAAAGTGACGATCGAACCCCATTTGATACGGCTCATGGAATGCCGTTCTGGGAGTATATGGGCAATAAGCAGAAAGATGGCGAGGTTTTTAACGCAGCCATGGCCAGCGATGCAAGGTTGGTGACGAGTATTGTGTTGGGCAAATATAAAGGCGTGTTTGACGGAGTTGAGTCGCTGGTTGATGTGGGAGGTGGCACTGGAACTGTGGCGAGAGCCATTGCTGAGGCTTTTCCAGAAATAGAATGTACTGTGTTTGATCTTCCCCAAGTTGTGGCTGACTTGAAAGGGAAAGACAACTTGAAATATGTTGAAGGGGACATGTTTGAAGCTATTCCTTCTGCTGATGCACTCCTGTTGAAGTGGATATTACATGATTGGAGTGATGAAGAATGTGTGAAGATACTGAAGAAATGCAAAGAGGCAATTACAGGCAATGAGAAAAAAGGGAAGGTGGAAAAGAGAGAGATGAAAAAGAGTGGGCTCAACTGA

Coding sequence (CDS)

ATGGATTTGGAGGGTGGAAATGTCGCAGTTGTTGCCGGCGACGAGCTCCTGGAAGCTCAAGCTCACATATGGAACCACATCTTCAACTTCATCAACTCCATGGCCCTCAAATGCGCAGTCCAGCTCGGCATACCAGACGCCATCCACCGCCATGGCTGCCACCCCATGCCCCTCTCTGAGCTTGTTTCAGCCCTCCTACTCAATCCCCGAAAGACCCATGACGTATACCGCTTAATGCGTATACTAACCCACTCGGGTTTCTTTACAATACAGAAAGTGCGCAAAAGTGGTGAACAAGAAGAAGAAGAAGAAGAAGAAGGATATGTGCTGACCAATTCATCTCGTCTTCTCCTTAAAGACAACCCCTCTGCTGTATCTCCTTTCTTGCTTTCCATGCTCGACCCAGCCCTCGTGGAGCCATGGCAGTTCCTCTCTACCTGGTTCCAAAGTGACGATCGAACCCCATTTGATACGGCTCATGGAATGCCGTTCTGGGAGTATATGGGCAATAAGCAGAAAGATGGCGAGGTTTTTAACGCAGCCATGGCCAGCGATGCAAGGTTGGTGACGAGTATTGTGTTGGGCAAATATAAAGGCGTGTTTGACGGAGTTGAGTCGCTGGTTGATGTGGGAGGTGGCACTGGAACTGTGGCGAGAGCCATTGCTGAGGCTTTTCCAGAAATAGAATGTACTGTGTTTGATCTTCCCCAAGTTGTGGCTGACTTGAAAGGGAAAGACAACTTGAAATATGTTGAAGGGGACATGTTTGAAGCTATTCCTTCTGCTGATGCACTCCTGTTGAAGTGGATATTACATGATTGGAGTGATGAAGAATGTGTGAAGATACTGAAGAAATGCAAAGAGGCAATTACAGGCAATGAGAAAAAAGGGAAGGTGGAAAAGAGAGAGATGAAAAAGAGTGGGCTCAACTGA

Protein sequence

MDLEGGNVAVVAGDELLEAQAHIWNHIFNFINSMALKCAVQLGIPDAIHRHGCHPMPLSELVSALLLNPRKTHDVYRLMRILTHSGFFTIQKVRKSGEQEEEEEEEGYVLTNSSRLLLKDNPSAVSPFLLSMLDPALVEPWQFLSTWFQSDDRTPFDTAHGMPFWEYMGNKQKDGEVFNAAMASDARLVTSIVLGKYKGVFDGVESLVDVGGGTGTVARAIAEAFPEIECTVFDLPQVVADLKGKDNLKYVEGDMFEAIPSADALLLKWILHDWSDEECVKILKKCKEAITGNEKKGKVEKREMKKSGLN

Homology

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BLAST of Sgr015334 vs. NCBI nr
Match: XP_022952337.1 (trans-resveratrol di-O-methyltransferase-like [Cucurbita moschata])

HSP 1 Score: 497.7 bits (1280), Expect = 7.4e-137
Identity = 240/299 (80.27%), Postives = 264/299 (88.29%), Query Frame = 0


Query: 1   MDLEGGNVAVVAGDELLEAQAHIWNHIFNFINSMALKCAVQLGIPDAIHRHGCHPMPLSE 60
           M+LEGG +A +AG+ELLEAQAHIWNH+FNFINSM LKCA+QLGIPDAIHRHG HPMPLS 
Sbjct: 1   MNLEGGKMAAIAGNELLEAQAHIWNHLFNFINSMTLKCAIQLGIPDAIHRHGPHPMPLSR 60

Query: 61  LVSALLLNPRKTHDVYRLMRILTHSGFFTIQKVRKSGEQEEEEEEEGYVLTNSSRLLLKD 120
           LVSAL L+P KT  +YRLMR+LTHSGFF  QK+ K+   EEEEEEEGYVLTNSSRLLLK 
Sbjct: 61  LVSALQLHPNKTQFIYRLMRLLTHSGFFVAQKLPKNSGLEEEEEEEGYVLTNSSRLLLKH 120

Query: 121 NPSAVSPFLLSMLDPALVEPWQFLSTWFQSDDRTPFDTAHGMPFWEYMGNKQKDGEVFNA 180
           NP AV+PFLLSML PALVEPWQ LSTWFQS+DRTPF TAHGMPFWE++GNK KDGE FNA
Sbjct: 121 NPCAVTPFLLSMLQPALVEPWQLLSTWFQSEDRTPFHTAHGMPFWEFLGNKVKDGEGFNA 180

Query: 181 AMASDARLVTSIVLGKYKGVFDGVESLVDVGGGTGTVARAIAEAFPEIECTVFDLPQVVA 240
            MASDARLV S++L K+KGVFDGV SLVDVGGGTGTV +AIAEAFP+IECTVFDLPQVV 
Sbjct: 181 GMASDARLVMSVLLDKHKGVFDGVASLVDVGGGTGTVGKAIAEAFPQIECTVFDLPQVVG 240

Query: 241 DLKGKDNLKYVEGDMFEAIPSADALLLKWILHDWSDEECVKILKKCKEAITGNEKKGKV 300
           +LKG+ NLKYVEGDMF+AIP ADALLLKWILHDWSD+ECVKILK CK AIT N  KGKV
Sbjct: 241 ELKGEGNLKYVEGDMFKAIPPADALLLKWILHDWSDQECVKILKNCKAAITSNGYKGKV 299

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BLAST of Sgr015334 vs. NCBI nr
Match: KAG6572273.1 (Trans-resveratrol di-O-methyltransferase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 491.9 bits (1265), Expect = 4.1e-135
Identity = 240/300 (80.00%), Postives = 265/300 (88.33%), Query Frame = 0


Query: 1   MDLEGGNVAVVAGDELLEAQAHIWNHIFNFINSMALKCAVQLGIPDAIHRHGCHPMPLSE 60
           M+LEGG +A +AG+ELLEAQAHIWNH+FNFINSM LKCA+QLGIPDAIHRHG +PMPLS 
Sbjct: 1   MNLEGGKMAAIAGNELLEAQAHIWNHLFNFINSMTLKCAIQLGIPDAIHRHGPNPMPLSR 60

Query: 61  LVSALLLNPRKTHDVYRLMRILTHSGFFTIQKVRK-SGEQEEEEEEEGYVLTNSSRLLLK 120
           LVSAL L+P KT  +YRLMR+LTHSGFF  QK+ K SG +EEEEEEEGYVLTNSSRLLLK
Sbjct: 61  LVSALQLHPNKTQFIYRLMRLLTHSGFFVAQKLPKNSGLEEEEEEEEGYVLTNSSRLLLK 120

Query: 121 DNPSAVSPFLLSMLDPALVEPWQFLSTWFQSDDRTPFDTAHGMPFWEYMGNKQKDGEVFN 180
            NP  V+PFLLSML PALVEPWQ LSTWFQS+DRTPF TAHGMP WE++GNK KDGE FN
Sbjct: 121 HNPCGVTPFLLSMLQPALVEPWQILSTWFQSEDRTPFHTAHGMPLWEFLGNKVKDGEGFN 180

Query: 181 AAMASDARLVTSIVLGKYKGVFDGVESLVDVGGGTGTVARAIAEAFPEIECTVFDLPQVV 240
             MASDARLV S++L K+KGVFDGV SLVDVGGGTGTVA+AIA+AFP+IECTV DLPQVV
Sbjct: 181 EGMASDARLVMSVLLDKHKGVFDGVASLVDVGGGTGTVAKAIAQAFPQIECTVLDLPQVV 240

Query: 241 ADLKGKDNLKYVEGDMFEAIPSADALLLKWILHDWSDEECVKILKKCKEAITGNEKKGKV 300
           A+LKG+ NLKYVEGDMF+AIPSADALLLKWILHDWSDEECVKILK CK AIT N  KGKV
Sbjct: 241 AELKGEGNLKYVEGDMFKAIPSADALLLKWILHDWSDEECVKILKNCKAAITSNGYKGKV 300

[Close]

BLAST of Sgr015334 vs. NCBI nr
Match: XP_022969140.1 (trans-resveratrol di-O-methyltransferase-like [Cucurbita maxima])

HSP 1 Score: 486.1 bits (1250), Expect = 2.2e-133
Identity = 235/299 (78.60%), Postives = 260/299 (86.96%), Query Frame = 0


Query: 1   MDLEGGNVAVVAGDELLEAQAHIWNHIFNFINSMALKCAVQLGIPDAIHRHGCHPMPLSE 60
           M+LEGG +A +AG+ELLEAQAHIWNH+FNFINSM LKCA+QLGIPDAIHRH  HPMPLS 
Sbjct: 1   MNLEGGKMAAIAGNELLEAQAHIWNHLFNFINSMTLKCAIQLGIPDAIHRHEPHPMPLSR 60

Query: 61  LVSALLLNPRKTHDVYRLMRILTHSGFFTIQKVRKSGEQEEEEEEEGYVLTNSSRLLLKD 120
           LVSAL L+P KT  +YRLMR+LTHSGFF  QKV K+  +EEEEEEEGYVLTNSSRLLLK 
Sbjct: 61  LVSALQLHPNKTQFIYRLMRLLTHSGFFVAQKVPKNSGKEEEEEEEGYVLTNSSRLLLKH 120

Query: 121 NPSAVSPFLLSMLDPALVEPWQFLSTWFQSDDRTPFDTAHGMPFWEYMGNKQKDGEVFNA 180
           NP   +PFLLSML PALVEPWQ LS WFQS+DRTPF TAHGMP WE++GNK KDGE FNA
Sbjct: 121 NPCGATPFLLSMLQPALVEPWQLLSIWFQSEDRTPFHTAHGMPLWEFLGNKVKDGEGFNA 180

Query: 181 AMASDARLVTSIVLGKYKGVFDGVESLVDVGGGTGTVARAIAEAFPEIECTVFDLPQVVA 240
            MASDARLV S++L K+KGVFDGV SLVDVGGGTGTVA+AIAEAFP+IECTV DLPQVV 
Sbjct: 181 GMASDARLVMSVLLDKHKGVFDGVASLVDVGGGTGTVAKAIAEAFPQIECTVLDLPQVVG 240

Query: 241 DLKGKDNLKYVEGDMFEAIPSADALLLKWILHDWSDEECVKILKKCKEAITGNEKKGKV 300
           +LKG+ NLKYVEGDMF+AIPSADALLLKWILHDWSD ECV+ILK CK AIT +  KGKV
Sbjct: 241 ELKGEGNLKYVEGDMFKAIPSADALLLKWILHDWSDPECVRILKNCKAAITSHGYKGKV 299

[Close]

BLAST of Sgr015334 vs. NCBI nr
Match: XP_022952338.1 (probable O-methyltransferase 3 [Cucurbita moschata])

HSP 1 Score: 481.9 bits (1239), Expect = 4.2e-132
Identity = 229/299 (76.59%), Postives = 260/299 (86.96%), Query Frame = 0


Query: 1   MDLEGGNVAVVAGDELLEAQAHIWNHIFNFINSMALKCAVQLGIPDAIHRHGCHPMPLSE 60
           M+ EG  + ++AGDE+LEAQAHIWNH+FNFINSM LKCAV+LGIPD IHRH  HPMPLS 
Sbjct: 1   MNFEGEKMPILAGDEMLEAQAHIWNHLFNFINSMTLKCAVELGIPDTIHRHAPHPMPLSH 60

Query: 61  LVSALLLNPRKTHDVYRLMRILTHSGFFTIQKVRKSGEQEEEEEEEGYVLTNSSRLLLKD 120
           LVSAL L+  KT  +YRLMR+LTHSGFF +QK+ K+  ++EEEEE+GYVLTNSSRLLLK 
Sbjct: 61  LVSALQLHSNKTQFIYRLMRLLTHSGFFVVQKLPKNNGKQEEEEEDGYVLTNSSRLLLKH 120

Query: 121 NPSAVSPFLLSMLDPALVEPWQFLSTWFQSDDRTPFDTAHGMPFWEYMGNKQKDGEVFNA 180
           NP AV+PFLLSML PALVEPWQ LS WFQS+DRTPF TAHGMPFWE++GNK KDGE FN 
Sbjct: 121 NPCAVTPFLLSMLQPALVEPWQLLSIWFQSEDRTPFHTAHGMPFWEFLGNKVKDGEGFNE 180

Query: 181 AMASDARLVTSIVLGKYKGVFDGVESLVDVGGGTGTVARAIAEAFPEIECTVFDLPQVVA 240
            MASDARLV S++L K+KGVFDGV SLVDVGGGTGTVA+AIAEAFP+IECTVFDLPQVV 
Sbjct: 181 GMASDARLVMSVLLDKHKGVFDGVASLVDVGGGTGTVAKAIAEAFPQIECTVFDLPQVVG 240

Query: 241 DLKGKDNLKYVEGDMFEAIPSADALLLKWILHDWSDEECVKILKKCKEAITGNEKKGKV 300
           +LKG+ NLKYVEGDMF+AIP AD L+LKWILHDW+D+EC KILKKCKEAI  N KKGKV
Sbjct: 241 ELKGEGNLKYVEGDMFKAIPPADVLVLKWILHDWNDDECAKILKKCKEAIRSNGKKGKV 299

[Close]

BLAST of Sgr015334 vs. NCBI nr
Match: XP_023554523.1 (trans-resveratrol di-O-methyltransferase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 478.8 bits (1231), Expect = 3.6e-131
Identity = 234/299 (78.26%), Postives = 260/299 (86.96%), Query Frame = 0


Query: 1   MDLEGGNVAVVAGDELLEAQAHIWNHIFNFINSMALKCAVQLGIPDAIHRHGCHPMPLSE 60
           M+LEGG +A +AG+ELLEAQAHIWNH+FNFINSM LKCA+QLGIPDAIHRHG +PMPLS 
Sbjct: 1   MNLEGGKMAAIAGNELLEAQAHIWNHLFNFINSMTLKCAIQLGIPDAIHRHGPNPMPLSR 60

Query: 61  LVSALLLNPRKTHDVYRLMRILTHSGFFTIQKVRKSGEQEEEEEEEGYVLTNSSRLLLKD 120
           LVSAL L+  KT  +YRLMR+LTHSGFF  QK+ K+    EEEEEEGYVLTNSSRLLLK 
Sbjct: 61  LVSALQLHSNKTQFIYRLMRLLTHSGFFVAQKLPKNSGL-EEEEEEGYVLTNSSRLLLKH 120

Query: 121 NPSAVSPFLLSMLDPALVEPWQFLSTWFQSDDRTPFDTAHGMPFWEYMGNKQKDGEVFNA 180
           NP  V+PFLLSML PALVEPWQ LSTWFQS+DRTPF TAHGMP WE++GNK KDGE FN 
Sbjct: 121 NPCGVTPFLLSMLQPALVEPWQLLSTWFQSEDRTPFHTAHGMPLWEFLGNKVKDGEGFNE 180

Query: 181 AMASDARLVTSIVLGKYKGVFDGVESLVDVGGGTGTVARAIAEAFPEIECTVFDLPQVVA 240
            MASDARLV S++L K+K VFDGV SLVDVGGGTGTVA+AIA+AFP+IECTV DLPQVVA
Sbjct: 181 GMASDARLVMSVLLDKHKAVFDGVASLVDVGGGTGTVAKAIAQAFPQIECTVLDLPQVVA 240

Query: 241 DLKGKDNLKYVEGDMFEAIPSADALLLKWILHDWSDEECVKILKKCKEAITGNEKKGKV 300
           +LKG+ NLKYVEGDMF+AIPSADALLLKWILHDWSDEECV+ILK CK AIT N  KGKV
Sbjct: 241 ELKGEGNLKYVEGDMFKAIPSADALLLKWILHDWSDEECVRILKNCKAAITSNGYKGKV 298

[Close]

BLAST of Sgr015334 vs. ExPASy Swiss-Prot
Match: B0ZB57 (Probable O-methyltransferase 3 OS=Humulus lupulus OX=3486 GN=OMT3 PE=2 SV=1)

HSP 1 Score: 379.8 bits (974), Expect = 3.0e-104
Identity = 191/292 (65.41%), Postives = 232/292 (79.45%), Query Frame = 0


Query: 15  ELLEAQAHIWNHIFNFINSMALKCAVQLGIPDAIHRHGCHPMPLSELVSALLLNPRKTHD 74
           E+L AQAHIWN IFNFINSM+LKCA+QLGIPD I+ HG  PM +S+L  AL +N +K+  
Sbjct: 25  EILGAQAHIWNQIFNFINSMSLKCAIQLGIPDIINNHG-KPMTISQLTLALPINRKKSPC 84

Query: 75  VYRLMRILTHSGFFTIQKVRKSGEQEEEEEEEGYVLTNSSRLLLKDNPSAVSPFLLSMLD 134
           VYRLMRIL HSGFF +QK      +E   EEEGYV+T++S+LLLKDNP +V+PFLL+MLD
Sbjct: 85  VYRLMRILIHSGFFALQKAEVG--EEGGGEEEGYVITDASKLLLKDNPMSVTPFLLAMLD 144

Query: 135 PALVEPWQFLSTWFQSDDRTPFDTAHGMPFWEYMGNKQKDGEVFNAAMASDARLVTSIVL 194
           P + +PW FLS WFQ+ D TPFDTA+GM FW+Y  ++ K    FN AMASDARLVTS+V+
Sbjct: 145 PVMTKPWDFLSNWFQNGDPTPFDTANGMAFWDYGSHEPKLARFFNDAMASDARLVTSVVI 204

Query: 195 GKYKGVFDGVESLVDVGGGTGTVARAIAEAFPEIECTVFDLPQVVADLKGKDNLKYVEGD 254
            K KGVF+GVESLVDVGGGTGTVA +IA AFP I+CTVFDLP VVADL+G +NL +V GD
Sbjct: 205 EKCKGVFEGVESLVDVGGGTGTVASSIAAAFPHIQCTVFDLPHVVADLQGGNNLNFVGGD 264

Query: 255 MFEAIPSADALLLKWILHDWSDEECVKILKKCKEAIT-GNEKKGKVEKREMK 306
           MF  +P+ + +LLKWILHDW+DEE VKILKKCKEAI+  N+K GKV   +MK
Sbjct: 265 MFVDVPATEVVLLKWILHDWNDEESVKILKKCKEAISKSNKKGGKVIIIDMK 313

[Close]

BLAST of Sgr015334 vs. ExPASy Swiss-Prot
Match: B6VJS4 (Trans-resveratrol di-O-methyltransferase OS=Vitis vinifera OX=29760 GN=ROMT PE=1 SV=2)

HSP 1 Score: 364.0 bits (933), Expect = 1.7e-99
Identity = 182/299 (60.87%), Postives = 223/299 (74.58%), Query Frame = 0


Query: 1   MDLEGGNVAVVAGDELLEAQAHIWNHIFNFINSMALKCAVQLGIPDAIHRHGCHPMPLSE 60
           MDL  G    V   ELL AQAH+WNHIFNFI SM+LKCA+QLGIPD IH HG  PM L E
Sbjct: 1   MDLANG----VISAELLHAQAHVWNHIFNFIKSMSLKCAIQLGIPDIIHNHG-KPMTLPE 60

Query: 61  LVSALLLNPRKTHDVYRLMRILTHSGFFTIQKVRKSGEQEEEEEEEGYVLTNSSRLLLKD 120
           LV+ L ++P+++  VYRLMRIL HSGF   Q+V      ++ +EEEGYVLT++SRLLL D
Sbjct: 61  LVAKLPVHPKRSQCVYRLMRILVHSGFLAAQRV------QQGKEEEGYVLTDASRLLLMD 120

Query: 121 NPSAVSPFLLSMLDPALVEPWQFLSTWFQSDDRTPFDTAHGMPFWEYMGNKQKDGEVFNA 180
           +  ++ P +L+MLDP L +PW +LS WFQ+DD TPF TA+   FW+Y G++ +    FN 
Sbjct: 121 DSLSIRPLVLAMLDPILTKPWHYLSAWFQNDDPTPFHTAYERSFWDYAGHEPQLNNSFNE 180

Query: 181 AMASDARLVTSIVLGKYKGVFDGVESLVDVGGGTGTVARAIAEAFPEIECTVFDLPQVVA 240
           AMASDARL+TS++L + +GVF G+ SLVDVGGGTG VA+AIA AFP + CTV DL  VVA
Sbjct: 181 AMASDARLLTSVLLKEGQGVFAGLNSLVDVGGGTGKVAKAIANAFPHLNCTVLDLSHVVA 240

Query: 241 DLKGKDNLKYVEGDMFEAIPSADALLLKWILHDWSDEECVKILKKCKEAITGNEKKGKV 300
            L+G  NL Y  GDMFEAIP ADA+LLKWILHDWS+EECVKILK+C+EAI   E  GKV
Sbjct: 241 GLQGSKNLNYFAGDMFEAIPPADAILLKWILHDWSNEECVKILKRCREAIPSKENGGKV 288

[Close]

BLAST of Sgr015334 vs. ExPASy Swiss-Prot
Match: A0A0N9HMN6 (Pluviatolide O-methyltransferase OS=Sinopodophyllum hexandrum OX=93608 GN=OMT3 PE=1 SV=1)

HSP 1 Score: 342.8 bits (878), Expect = 4.0e-93
Identity = 170/302 (56.29%), Postives = 217/302 (71.85%), Query Frame = 0


Query: 1   MDLE--GGNVAVVAGDELLEAQAHIWNHIFNFINSMALKCAVQLGIPDAIHRHGCHPMPL 60
           MDLE   GN    +G+ELL AQAHI+NHIFNFI+SMALKCAV+L IP+ +H H    + L
Sbjct: 7   MDLEIRNGNGYGDSGEELLAAQAHIYNHIFNFISSMALKCAVELNIPEILHNHQPKAVTL 66

Query: 61  SELVSALLLNPRKTHDVYRLMRILTHSGFFTIQKVRKSGEQEEEEEEEGYVLTNSSRLLL 120
           SELV AL +   K+  +YRL+RIL HSGFF I K++  G      +EEGY+ T SS+LLL
Sbjct: 67  SELVQALQIPQAKSACLYRLLRILVHSGFFAITKIQSEG------DEEGYLPTLSSKLLL 126

Query: 121 KDNPSAVSPFLLSMLDPALVEPWQFLSTWF-QSDDRTPFDTAHGMPFWEYMGNKQKDGEV 180
           K++P ++SP LL +++P +V P  F S WF +SDD TPF+  HG   W+Y G      E+
Sbjct: 127 KNHPMSMSPCLLGLVNPTMVAPMHFFSDWFKRSDDMTPFEATHGASLWKYFGETPHMAEI 186

Query: 181 FNAAMASDARLVTSIVLGKYKGVFDGVESLVDVGGGTGTVARAIAEAFPEIECTVFDLPQ 240
           FN AM  + RL  S+VL + KG  +G+ SLVDVGGGTG V RAIAEAFP ++CTV DLPQ
Sbjct: 187 FNEAMGCETRLAMSVVLKECKGKLEGISSLVDVGGGTGNVGRAIAEAFPNVKCTVLDLPQ 246

Query: 241 VVADLKGKDNLKYVEGDMFEAIPSADALLLKWILHDWSDEECVKILKKCKEAITGNEKKG 300
           VV +LKG +NL++V GDMF+ IP AD + LKWILHDW+DEEC+KILK+CKEAI   E+ G
Sbjct: 247 VVGNLKGSNNLEFVSGDMFQFIPPADVVFLKWILHDWNDEECIKILKRCKEAIPSKEEGG 302

[Close]

BLAST of Sgr015334 vs. ExPASy Swiss-Prot
Match: Q6VMW0 (8-hydroxyquercetin 8-O-methyltransferase OS=Mentha piperita OX=34256 GN=OMT2 PE=1 SV=1)

HSP 1 Score: 335.1 bits (858), Expect = 8.4e-91
Identity = 164/278 (58.99%), Postives = 208/278 (74.82%), Query Frame = 0


Query: 15  ELLEAQAHIWNHIFNFINSMALKCAVQLGIPDAIHRHGCHPMPLSELVSALLLNPRKTHD 74
           ELLEAQAH+WNHI+++INSM+LKCA+QLGIPDAIH+HG +P+ LS+L  AL +N  K+H 
Sbjct: 12  ELLEAQAHVWNHIYSYINSMSLKCAIQLGIPDAIHKHG-NPITLSQLADALNINKAKSHG 71

Query: 75  VYRLMRILTHSGFFTIQKVRKSGE-QEEEEEEEGYVLTNSSRLLLKDNPSAVSPFLLSML 134
           ++RLMRIL HSGFF   KV+   E ++EEEEE+ Y LT +SRLLL+  P +V+PF L+M 
Sbjct: 72  LFRLMRILVHSGFFDKVKVKVKVEGEDEEEEEDAYSLTPASRLLLRSEPLSVAPFALAMS 131

Query: 135 DPALVEPWQFLSTWFQSDDRTPFDTAHGMPFWEYMGNKQKDGEVFNAAMASDARLVTSIV 194
           DP   E W  LS WF++D    FDT +GM F EY     +   +FN AMA DA  V SI+
Sbjct: 132 DPVYTETWHHLSEWFRNDAVAAFDTKYGMTFPEYAVADDRLNVLFNEAMACDAGFVNSIL 191

Query: 195 LGKYKGVFDGVESLVDVGGGTGTVARAIAEAFPEIECTVFDLPQVVADLKGKDNLKYVEG 254
             + + +FDG+ES+VDVGGGTG  A+ IA AFP +ECTV DLP VV  LKG +NL +V G
Sbjct: 192 TTECREIFDGLESMVDVGGGTGATAKGIAAAFPGMECTVLDLPNVVGGLKGSENLSFVSG 251

Query: 255 DMFEAIPSADALLLKWILHDWSDEECVKILKKCKEAIT 292
           DMF+ IP ADA+ +K+ILHDW+DEECVKILKKCKEAI+
Sbjct: 252 DMFDFIPHADAIFMKFILHDWNDEECVKILKKCKEAIS 288

[Close]

BLAST of Sgr015334 vs. ExPASy Swiss-Prot
Match: B0EXJ8 (Tabersonine 16-O-methyltransferase OS=Catharanthus roseus OX=4058 GN=16OMT PE=1 SV=1)

HSP 1 Score: 295.8 bits (756), Expect = 5.6e-79
Identity = 152/293 (51.88%), Postives = 207/293 (70.65%), Query Frame = 0


Query: 11  VAGDELLEAQAHIWNHIFNFINSMALKCAVQLGIPDAIHRHGCHPMPLSELVSALL--LN 70
           V  +E   AQA IW+   +FI S +LKCAV+LGIPD I  HG  P+ LSEL +AL+  ++
Sbjct: 3   VQSEEFRGAQAQIWSQSCSFITSASLKCAVKLGIPDTIDNHG-KPITLSELTNALVPPVH 62

Query: 71  PRKTHDVYRLMRILTHSGFFTIQKVRKSGEQEEEEEEEGYVLTNSSRLLLKDNPSAVSPF 130
           P K   +YRLMR+L  +GF        S EQ + E E  Y LT SSR+LLK  P  +   
Sbjct: 63  PSKAPFIYRLMRVLAKNGFC-------SEEQLDGETEPLYSLTPSSRILLKKEPLNLRGI 122

Query: 131 LLSMLDPALVEPWQFLSTWFQSDD--RTPFDTAHGMPFWEYMGNKQKDGEVFNAAMASDA 190
           +L+M DP  ++ W+ LS W+Q++D   T F+TAHG  FW Y     +  E FN AMASD+
Sbjct: 123 VLTMADPVQLKAWESLSDWYQNEDDSSTAFETAHGKNFWGYSSEHMEHAEFFNEAMASDS 182

Query: 191 RLVTSIVLGKYKGVFDGVESLVDVGGGTGTVARAIAEAFPEIECTVFDLPQVVADLKGKD 250
           +L++ +++G+YK +F+G+ SLVD+GGGTGT+A+AIA+ FP+++CTVFDLP VVA+L+ K+
Sbjct: 183 QLISKLLIGEYKFLFEGLASLVDIGGGTGTIAKAIAKNFPQLKCTVFDLPHVVANLESKE 242

Query: 251 NLKYVEGDMFEAIPSADALLLKWILHDWSDEECVKILKKCKEAITGNEKKGKV 300
           N+++V GDMFE IPSA+A+ LKWILHDW+DE+CVKILK CK+AI    K GKV
Sbjct: 243 NVEFVAGDMFEKIPSANAIFLKWILHDWNDEDCVKILKSCKKAIPA--KGGKV 285

[Close]

BLAST of Sgr015334 vs. ExPASy TrEMBL
Match: A0A6J1GK49 (trans-resveratrol di-O-methyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111455045 PE=3 SV=1)

HSP 1 Score: 497.7 bits (1280), Expect = 3.6e-137
Identity = 240/299 (80.27%), Postives = 264/299 (88.29%), Query Frame = 0


Query: 1   MDLEGGNVAVVAGDELLEAQAHIWNHIFNFINSMALKCAVQLGIPDAIHRHGCHPMPLSE 60
           M+LEGG +A +AG+ELLEAQAHIWNH+FNFINSM LKCA+QLGIPDAIHRHG HPMPLS 
Sbjct: 1   MNLEGGKMAAIAGNELLEAQAHIWNHLFNFINSMTLKCAIQLGIPDAIHRHGPHPMPLSR 60

Query: 61  LVSALLLNPRKTHDVYRLMRILTHSGFFTIQKVRKSGEQEEEEEEEGYVLTNSSRLLLKD 120
           LVSAL L+P KT  +YRLMR+LTHSGFF  QK+ K+   EEEEEEEGYVLTNSSRLLLK 
Sbjct: 61  LVSALQLHPNKTQFIYRLMRLLTHSGFFVAQKLPKNSGLEEEEEEEGYVLTNSSRLLLKH 120

Query: 121 NPSAVSPFLLSMLDPALVEPWQFLSTWFQSDDRTPFDTAHGMPFWEYMGNKQKDGEVFNA 180
           NP AV+PFLLSML PALVEPWQ LSTWFQS+DRTPF TAHGMPFWE++GNK KDGE FNA
Sbjct: 121 NPCAVTPFLLSMLQPALVEPWQLLSTWFQSEDRTPFHTAHGMPFWEFLGNKVKDGEGFNA 180

Query: 181 AMASDARLVTSIVLGKYKGVFDGVESLVDVGGGTGTVARAIAEAFPEIECTVFDLPQVVA 240
            MASDARLV S++L K+KGVFDGV SLVDVGGGTGTV +AIAEAFP+IECTVFDLPQVV 
Sbjct: 181 GMASDARLVMSVLLDKHKGVFDGVASLVDVGGGTGTVGKAIAEAFPQIECTVFDLPQVVG 240

Query: 241 DLKGKDNLKYVEGDMFEAIPSADALLLKWILHDWSDEECVKILKKCKEAITGNEKKGKV 300
           +LKG+ NLKYVEGDMF+AIP ADALLLKWILHDWSD+ECVKILK CK AIT N  KGKV
Sbjct: 241 ELKGEGNLKYVEGDMFKAIPPADALLLKWILHDWSDQECVKILKNCKAAITSNGYKGKV 299

[Close]

BLAST of Sgr015334 vs. ExPASy TrEMBL
Match: A0A6J1HZ45 (trans-resveratrol di-O-methyltransferase-like OS=Cucurbita maxima OX=3661 GN=LOC111468224 PE=3 SV=1)

HSP 1 Score: 486.1 bits (1250), Expect = 1.1e-133
Identity = 235/299 (78.60%), Postives = 260/299 (86.96%), Query Frame = 0


Query: 1   MDLEGGNVAVVAGDELLEAQAHIWNHIFNFINSMALKCAVQLGIPDAIHRHGCHPMPLSE 60
           M+LEGG +A +AG+ELLEAQAHIWNH+FNFINSM LKCA+QLGIPDAIHRH  HPMPLS 
Sbjct: 1   MNLEGGKMAAIAGNELLEAQAHIWNHLFNFINSMTLKCAIQLGIPDAIHRHEPHPMPLSR 60

Query: 61  LVSALLLNPRKTHDVYRLMRILTHSGFFTIQKVRKSGEQEEEEEEEGYVLTNSSRLLLKD 120
           LVSAL L+P KT  +YRLMR+LTHSGFF  QKV K+  +EEEEEEEGYVLTNSSRLLLK 
Sbjct: 61  LVSALQLHPNKTQFIYRLMRLLTHSGFFVAQKVPKNSGKEEEEEEEGYVLTNSSRLLLKH 120

Query: 121 NPSAVSPFLLSMLDPALVEPWQFLSTWFQSDDRTPFDTAHGMPFWEYMGNKQKDGEVFNA 180
           NP   +PFLLSML PALVEPWQ LS WFQS+DRTPF TAHGMP WE++GNK KDGE FNA
Sbjct: 121 NPCGATPFLLSMLQPALVEPWQLLSIWFQSEDRTPFHTAHGMPLWEFLGNKVKDGEGFNA 180

Query: 181 AMASDARLVTSIVLGKYKGVFDGVESLVDVGGGTGTVARAIAEAFPEIECTVFDLPQVVA 240
            MASDARLV S++L K+KGVFDGV SLVDVGGGTGTVA+AIAEAFP+IECTV DLPQVV 
Sbjct: 181 GMASDARLVMSVLLDKHKGVFDGVASLVDVGGGTGTVAKAIAEAFPQIECTVLDLPQVVG 240

Query: 241 DLKGKDNLKYVEGDMFEAIPSADALLLKWILHDWSDEECVKILKKCKEAITGNEKKGKV 300
           +LKG+ NLKYVEGDMF+AIPSADALLLKWILHDWSD ECV+ILK CK AIT +  KGKV
Sbjct: 241 ELKGEGNLKYVEGDMFKAIPSADALLLKWILHDWSDPECVRILKNCKAAITSHGYKGKV 299

[Close]

BLAST of Sgr015334 vs. ExPASy TrEMBL
Match: A0A6J1GKA3 (probable O-methyltransferase 3 OS=Cucurbita moschata OX=3662 GN=LOC111455047 PE=3 SV=1)

HSP 1 Score: 481.9 bits (1239), Expect = 2.0e-132
Identity = 229/299 (76.59%), Postives = 260/299 (86.96%), Query Frame = 0


Query: 1   MDLEGGNVAVVAGDELLEAQAHIWNHIFNFINSMALKCAVQLGIPDAIHRHGCHPMPLSE 60
           M+ EG  + ++AGDE+LEAQAHIWNH+FNFINSM LKCAV+LGIPD IHRH  HPMPLS 
Sbjct: 1   MNFEGEKMPILAGDEMLEAQAHIWNHLFNFINSMTLKCAVELGIPDTIHRHAPHPMPLSH 60

Query: 61  LVSALLLNPRKTHDVYRLMRILTHSGFFTIQKVRKSGEQEEEEEEEGYVLTNSSRLLLKD 120
           LVSAL L+  KT  +YRLMR+LTHSGFF +QK+ K+  ++EEEEE+GYVLTNSSRLLLK 
Sbjct: 61  LVSALQLHSNKTQFIYRLMRLLTHSGFFVVQKLPKNNGKQEEEEEDGYVLTNSSRLLLKH 120

Query: 121 NPSAVSPFLLSMLDPALVEPWQFLSTWFQSDDRTPFDTAHGMPFWEYMGNKQKDGEVFNA 180
           NP AV+PFLLSML PALVEPWQ LS WFQS+DRTPF TAHGMPFWE++GNK KDGE FN 
Sbjct: 121 NPCAVTPFLLSMLQPALVEPWQLLSIWFQSEDRTPFHTAHGMPFWEFLGNKVKDGEGFNE 180

Query: 181 AMASDARLVTSIVLGKYKGVFDGVESLVDVGGGTGTVARAIAEAFPEIECTVFDLPQVVA 240
            MASDARLV S++L K+KGVFDGV SLVDVGGGTGTVA+AIAEAFP+IECTVFDLPQVV 
Sbjct: 181 GMASDARLVMSVLLDKHKGVFDGVASLVDVGGGTGTVAKAIAEAFPQIECTVFDLPQVVG 240

Query: 241 DLKGKDNLKYVEGDMFEAIPSADALLLKWILHDWSDEECVKILKKCKEAITGNEKKGKV 300
           +LKG+ NLKYVEGDMF+AIP AD L+LKWILHDW+D+EC KILKKCKEAI  N KKGKV
Sbjct: 241 ELKGEGNLKYVEGDMFKAIPPADVLVLKWILHDWNDDECAKILKKCKEAIRSNGKKGKV 299

[Close]

BLAST of Sgr015334 vs. ExPASy TrEMBL
Match: A0A6J1GJY8 (probable O-methyltransferase 3 OS=Cucurbita moschata OX=3662 GN=LOC111455044 PE=3 SV=1)

HSP 1 Score: 466.8 bits (1200), Expect = 6.8e-128
Identity = 226/300 (75.33%), Postives = 258/300 (86.00%), Query Frame = 0


Query: 1   MDLEGGN-VAVVAGDELLEAQAHIWNHIFNFINSMALKCAVQLGIPDAIHRHGCHPMPLS 60
           M+ E  N + +VAGDELLEAQAHIWNH+ NFINSM LKCAV+LGIPDAIHRHG HPMPLS
Sbjct: 5   MNFESENMLPIVAGDELLEAQAHIWNHLLNFINSMTLKCAVELGIPDAIHRHGPHPMPLS 64

Query: 61  ELVSALLLNPRKTHDVYRLMRILTHSGFFTIQKVRKSGEQEEEEEEEGYVLTNSSRLLLK 120
            LVSAL L+P KT  +YRLMR+LTHSGFF +QK+ K+   EEEEEEEGYVLTNSSRLLLK
Sbjct: 65  RLVSALQLHPHKTRFIYRLMRLLTHSGFFVLQKLPKN-SGEEEEEEEGYVLTNSSRLLLK 124

Query: 121 DNPSAVSPFLLSMLDPALVEPWQFLSTWFQSDDRTPFDTAHGMPFWEYMGNKQKDGEVFN 180
            NP  V+P LLS+L PALVEPW  LS WF+S+DRTPFDTAHG+ +WE+MG+K K G++FN
Sbjct: 125 YNPCGVTPLLLSILQPALVEPWHLLSVWFKSEDRTPFDTAHGISYWEFMGSKPKHGDIFN 184

Query: 181 AAMASDARLVTSIVLGKYKGVFDGVESLVDVGGGTGTVARAIAEAFPEIECTVFDLPQVV 240
           A MASDARLV S++L K+KGVFDGV SLVDVGGGTGTV +AIAEAFP+IEC VFDLPQVV
Sbjct: 185 AGMASDARLVMSVLLDKHKGVFDGVASLVDVGGGTGTVGKAIAEAFPQIECAVFDLPQVV 244

Query: 241 ADLKGKDNLKYVEGDMFEAIPSADALLLKWILHDWSDEECVKILKKCKEAITGNEKKGKV 300
            +LKG+ NLKYVEGDMF+AIP AD L+LKWILHDW+DE+CVKILK CKEAIT N KKGKV
Sbjct: 245 GELKGEGNLKYVEGDMFKAIPPADVLVLKWILHDWNDEDCVKILKNCKEAITSNGKKGKV 303

[Close]

BLAST of Sgr015334 vs. ExPASy TrEMBL
Match: A0A6J1C1F3 (probable O-methyltransferase 3 OS=Momordica charantia OX=3673 GN=LOC111007572 PE=3 SV=1)

HSP 1 Score: 460.3 bits (1183), Expect = 6.4e-126
Identity = 226/299 (75.59%), Postives = 254/299 (84.95%), Query Frame = 0


Query: 1   MDLEGGNVAVVAGDELLEAQAHIWNHIFNFINSMALKCAVQLGIPDAIHRHGCHPMPLSE 60
           M++E G    V   ELLEAQAHIWNHIFNFINSMALKCAVQLGIPDAIHRHG +PMPLS+
Sbjct: 1   MNMELGGDIAVPSHELLEAQAHIWNHIFNFINSMALKCAVQLGIPDAIHRHGPNPMPLSQ 60

Query: 61  LVSALLLNPRKTHDVYRLMRILTHSGFFTIQKVRKSGEQEEEEEEEGYVLTNSSRLLLKD 120
           LVSAL L+P+KT  +YRLMR+LTHS FF   K+       +  EEE YVLTNSSRLLL D
Sbjct: 61  LVSALQLSPQKTQYIYRLMRLLTHSSFFVTHKM-----MTKNGEEEAYVLTNSSRLLLND 120

Query: 121 NPSAVSPFLLSMLDPALVEPWQFLSTWFQSDDRTPFDTAHGMPFWEYMGNKQKDGEVFNA 180
           NPSAV+PFLLSML+PALVEPWQ L+ W QS+DRTPF T HGMPFWE+   K KDG+VFN 
Sbjct: 121 NPSAVTPFLLSMLEPALVEPWQLLAAWLQSEDRTPFHTTHGMPFWEFFSEKPKDGDVFNV 180

Query: 181 AMASDARLVTSIVLGKYKGVFDGVESLVDVGGGTGTVARAIAEAFPEIECTVFDLPQVVA 240
           AMASDARLV SI+L KYKGVF+GV SLVDVGGGTGTVA+AIA+AFP+IEC VFDL QVV 
Sbjct: 181 AMASDARLVMSILLDKYKGVFEGVGSLVDVGGGTGTVAKAIAKAFPQIECIVFDLHQVVG 240

Query: 241 DLKGKDNLKYVEGDMFEAIPSADALLLKWILHDWSDEECVKILKKCKEAITGNEKKGKV 300
           +LKG+DNLKYVEG+MFEA+PSADALLLKWILHDW+DEECV+ILKKCKEAIT N KKGK+
Sbjct: 241 NLKGEDNLKYVEGNMFEAVPSADALLLKWILHDWNDEECVEILKKCKEAITNNGKKGKM 294

[Close]

BLAST of Sgr015334 vs. TAIR 10
Match: AT4G35160.1 (O-methyltransferase family protein )

HSP 1 Score: 201.4 bits (511), Expect = 1.0e-51
Identity = 114/287 (39.72%), Postives = 161/287 (56.10%), Query Frame = 0


Query: 14  DELLEAQAHIWNHIFNFINSMALKCAVQLGIPDAIHRH-GCHPMPLSELVSALLLNPRKT 73
           DE  +A   IW ++F F +  A KCA+ L IP+AI  H    P+ L+EL SA+  +P   
Sbjct: 24  DEEAKASLDIWKYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLAELSSAVSASP--- 83

Query: 74  HDVYRLMRILTHSGFFTIQKVRKSGEQEEEEEEEGYVLTNSSRLLL--KDNPSAVSPFLL 133
             + R+MR L H G F  +   K G         GYV T  SR L+  + +  +++PF+L
Sbjct: 84  SHLRRIMRFLVHQGIFK-EIPTKDG------LATGYVNTPLSRRLMITRRDGKSLAPFVL 143

Query: 134 SMLDPALVEPWQFLSTWFQS----DDRTPFDTAHGMPFWEYMGNKQKDGEVFNAAMASDA 193
               P ++ PW  LS+   S        PFD  HG   W +  +     ++ N AMA DA
Sbjct: 144 FETTPEMLAPWLRLSSVVSSPVNGSTPPPFDAVHGKDVWSFAQDNPFLSDMINEAMACDA 203

Query: 194 RLVTSIVLGKYKGVFDGVESLVDVGGGTGTVARAIAEAFPEIECTVFDLPQVVADLKGKD 253
           R V   V G   G+FDGV ++VDVGGGTG     + + FP I+   FDLP V+   +  D
Sbjct: 204 RRVVPRVAGACHGLFDGVTTMVDVGGGTGETMGMLVKEFPWIKGFNFDLPHVIEVAEVLD 263

Query: 254 NLKYVEGDMFEAIPSADALLLKWILHDWSDEECVKILKKCKEAITGN 294
            ++ VEGDMF++IP+ DA+ +KW+LHDW D++C+KILK CKEA+  N
Sbjct: 264 GVENVEGDMFDSIPACDAIFIKWVLHDWGDKDCIKILKNCKEAVPPN 300

[Close]

BLAST of Sgr015334 vs. TAIR 10
Match: AT4G35150.1 (O-methyltransferase family protein )

HSP 1 Score: 171.4 bits (433), Expect = 1.1e-42
Identity = 102/281 (36.30%), Postives = 141/281 (50.18%), Query Frame = 0


Query: 14  DELLEAQAHIWNHIFNFINSMALKCAVQLGIPDAIHRH-GCHPMPLSELVSALLLNPRKT 73
           DE  +A   IW ++F F +  A KCA+ L IP+AI  H    P+ LSEL SA+  +P   
Sbjct: 10  DEEAKASLDIWRYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLSELSSAVSASP--- 69

Query: 74  HDVYRLMRILTHSGFFTIQKVRKSGEQEEEEEEEGYVLTNSSRLLLKDNPSAVSPFLLSM 133
             + R+MR L H G F  +   K G         GY  T  SR                 
Sbjct: 70  SHLRRIMRFLVHQGLFK-EVPTKDG------LATGYTNTPLSR----------------- 129

Query: 134 LDPALVEPWQFLSTWFQSDDRTPFDTAHGMPFWEYMGNKQKDGEVFNAAMASDARLVTSI 193
                               R      HG   W +  +     ++ N AMA DAR V   
Sbjct: 130 --------------------RMMITKLHGKDLWAFAQDNLCHSQLINEAMACDARRVVPR 189

Query: 194 VLGKYKGVFDGVESLVDVGGGTGTVARAIAEAFPEIECTVFDLPQVVADLKGKDNLKYVE 253
           V G  +G+FDGV ++VDVGGGTG     + + FP I+   FDLP V+   +  D ++ VE
Sbjct: 190 VAGACQGLFDGVATVVDVGGGTGETMGILVKEFPWIKGFNFDLPHVIEVAQVLDGVENVE 243

Query: 254 GDMFEAIPSADALLLKWILHDWSDEECVKILKKCKEAITGN 294
           GDMF++IP++DA+++KW+LHDW D++C+KILK CKEA+  N
Sbjct: 250 GDMFDSIPASDAVIIKWVLHDWGDKDCIKILKNCKEAVLPN 243

[Close]

BLAST of Sgr015334 vs. TAIR 10
Match: AT5G54160.1 (O-methyltransferase 1 )

HSP 1 Score: 152.9 bits (385), Expect = 4.2e-37
Identity = 92/263 (34.98%), Postives = 140/263 (53.23%), Query Frame = 0


Query: 34  MALKCAVQLGIPDAIHRHGCHPMPLSELVSAL-LLNPRKTHDVYRLMRILTHSGFFTIQK 93
           MALK A++L + + + ++G  PM  +E+ S L   NP     + R++R+LT     T   
Sbjct: 34  MALKSALELDLLEIMAKNG-SPMSPTEIASKLPTKNPEAPVMLDRILRLLTSYSVLTCSN 93

Query: 94  VRKSGEQEEEEEEEGYVLTNSSRLLLKDNPSAVSPFLLSMLDPALVEPWQFLSTWFQSDD 153
            + SG+  E     G V      L   ++  +++   L   D  L+E W  L      D 
Sbjct: 94  RKLSGDGVERIYGLGPV---CKYLTKNEDGVSIAALCLMNQDKVLMESWYHLKDAI-LDG 153

Query: 154 RTPFDTAHGMPFWEYMGNKQKDGEVFNAAMASDARLVTSIVLGKYKGVFDGVESLVDVGG 213
             PF+ A+GM  +EY G   +  +VFN  M++ + +    +L  YKG F+G+ SLVDVGG
Sbjct: 154 GIPFNKAYGMSAFEYHGTDPRFNKVFNNGMSNHSTITMKKILETYKG-FEGLTSLVDVGG 213

Query: 214 GTGTVARAIAEAFPEIECTVFDLPQVVADLKGKDNLKYVEGDMFEAIPSADALLLKWILH 273
           G G   + I   +P ++   FDLP V+ D      +++V GDMF ++P  DA+ +KWI H
Sbjct: 214 GIGATLKMIVSKYPNLKGINFDLPHVIEDAPSHPGIEHVGGDMFVSVPKGDAIFMKWICH 273

Query: 274 DWSDEECVKILKKCKEAITGNEK 296
           DWSDE CVK LK C E++  + K
Sbjct: 274 DWSDEHCVKFLKNCYESLPEDGK 290

[Close]

BLAST of Sgr015334 vs. TAIR 10
Match: AT1G77520.1 (O-methyltransferase family protein )

HSP 1 Score: 142.9 bits (359), Expect = 4.3e-34
Identity = 89/275 (32.36%), Postives = 135/275 (49.09%), Query Frame = 0


Query: 34  MALKCAVQLGIPDAIHRHG----CHPMPLSELVSALLLNPRKTHDVYRLMRILTHSGFFT 93
           M LK A +LG+ D I   G      P  ++  +     NP     + R++ +L       
Sbjct: 44  MVLKAAFELGVIDTIAAAGNDTWLSPCEIACSLPTKPTNPEAPVLLDRMLSLLVSHSILK 103

Query: 94  IQKVRKSGEQEEEEEEEGYVLTNSSRLLLKDNPSAVSPFLLSMLDPALVEPWQFLSTWFQ 153
            + +         + E  Y      +  L+D+    S   L M    L+    F  TW  
Sbjct: 104 CRMIETGENGRTGKIERVYAAEPVCKYFLRDSDGTGSLVPLFM----LLHTQVFFKTWTN 163

Query: 154 SDD-----RTPFDTAHGMPFWEYMGNKQKDGEVFNAAMASDARLVTSIVLGKYKGVFDGV 213
             D     R  F++AHGM  +EY+ + Q   E+FN AM+  + ++   VL  Y+G F+ V
Sbjct: 164 LKDVILEGRDAFNSAHGMKIFEYINSDQPFAELFNRAMSEPSTMIMKKVLDVYRG-FEDV 223

Query: 214 ESLVDVGGGTGTVARAIAEAFPEIECTVFDLPQVVADLKGKDNLKYVEGDMFEAIPSADA 273
            +LVDVGGG GTV   +   +P I+   FDL QV+        +++V GDMF  +P  DA
Sbjct: 224 NTLVDVGGGNGTVLGLVTSKYPHIKGVNFDLAQVLTQAPFYPGVEHVSGDMFVEVPKGDA 283

Query: 274 LLLKWILHDWSDEECVKILKKCKEAITGNEKKGKV 300
           + +KWILHDW DE+C+KILK C +++    +KGK+
Sbjct: 284 VFMKWILHDWGDEDCIKILKNCWKSL---PEKGKI 310

[Close]

BLAST of Sgr015334 vs. TAIR 10
Match: AT1G63140.2 (O-methyltransferase family protein )

HSP 1 Score: 139.4 bits (350), Expect = 4.8e-33
Identity = 97/293 (33.11%), Postives = 149/293 (50.85%), Query Frame = 0


Query: 14  DELLEAQAHIWNHIFNFINSMALKCAVQLGIPDAIHR--HGCHPMPLSELVSALLLNPRK 73
           ++ +  QA    H   F   M LK A++LG+ D I     G    P SE+   L   P  
Sbjct: 26  EDTVSLQAERILHAMTF--PMVLKTALELGVIDMITSVDDGVWLSP-SEIALGLPTKPTN 85

Query: 74  THDVYRLMRILTHSGFFTIQKVR--KSGEQ-EEEEEEEGYVLTNSSRLLLK--DNPSAVS 133
                 L R+L      +I K R  ++G+     + E  Y         L   D   +++
Sbjct: 86  PEAPVLLDRMLVLLASHSILKYRTVETGDNIGSRKTERVYAAEPVCTFFLNRGDGLGSLA 145

Query: 134 PFLLSMLDPALVEPWQFLSTWFQSDDRTPFDTAHGMPFWEYMGNKQKDGEVFNAAMASDA 193
              + +     ++PW+ L      + +  F +AHGM F+E +G+ ++  E+FN AM+  +
Sbjct: 146 TLFMVLQGEVCMKPWEHLKDMI-LEGKDAFTSAHGMRFFELIGSNEQFAEMFNRAMSEAS 205

Query: 194 RLVTSIVLGKYKGVFDGVESLVDVGGGTGTVARAIAEAFPEIECTVFDLPQVVADLKGKD 253
            L+   VL  YKG F+ V +LVDVGGG GT+   +   +P I+   FDL  V+A      
Sbjct: 206 TLIMKKVLEVYKG-FEDVNTLVDVGGGIGTIIGQVTSKYPHIKGINFDLASVLAHAPFNK 265

Query: 254 NLKYVEGDMFEAIPSADALLLKWILHDWSDEECVKILKKCKEAITGNEKKGKV 300
            +++V GDMF+ IP  DA+ +KWILHDW+DE+CVKILK   +++    +KGKV
Sbjct: 266 GVEHVSGDMFKEIPKGDAIFMKWILHDWTDEDCVKILKNYWKSL---PEKGKV 310

The following BLAST results are available for this feature:

BLAST of Sgr015334 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022952337.1	7.4e-137	80.27	trans-resveratrol di-O-methyltransferase-like [Cucurbita moschata]	[more]
KAG6572273.1	4.1e-135	80.00	Trans-resveratrol di-O-methyltransferase, partial [Cucurbita argyrosperma subsp....	[more]
XP_022969140.1	2.2e-133	78.60	trans-resveratrol di-O-methyltransferase-like [Cucurbita maxima]	[more]
XP_022952338.1	4.2e-132	76.59	probable O-methyltransferase 3 [Cucurbita moschata]	[more]
XP_023554523.1	3.6e-131	78.26	trans-resveratrol di-O-methyltransferase-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Sgr015334 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
B0ZB57	3.0e-104	65.41	Probable O-methyltransferase 3 OS=Humulus lupulus OX=3486 GN=OMT3 PE=2 SV=1	[more]
B6VJS4	1.7e-99	60.87	Trans-resveratrol di-O-methyltransferase OS=Vitis vinifera OX=29760 GN=ROMT PE=1...	[more]
A0A0N9HMN6	4.0e-93	56.29	Pluviatolide O-methyltransferase OS=Sinopodophyllum hexandrum OX=93608 GN=OMT3 P...	[more]
Q6VMW0	8.4e-91	58.99	8-hydroxyquercetin 8-O-methyltransferase OS=Mentha piperita OX=34256 GN=OMT2 PE=...	[more]
B0EXJ8	5.6e-79	51.88	Tabersonine 16-O-methyltransferase OS=Catharanthus roseus OX=4058 GN=16OMT PE=1 ...	[more]

BLAST of Sgr015334 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GK49	3.6e-137	80.27	trans-resveratrol di-O-methyltransferase-like OS=Cucurbita moschata OX=3662 GN=L...	[more]
A0A6J1HZ45	1.1e-133	78.60	trans-resveratrol di-O-methyltransferase-like OS=Cucurbita maxima OX=3661 GN=LOC...	[more]
A0A6J1GKA3	2.0e-132	76.59	probable O-methyltransferase 3 OS=Cucurbita moschata OX=3662 GN=LOC111455047 PE=...	[more]
A0A6J1GJY8	6.8e-128	75.33	probable O-methyltransferase 3 OS=Cucurbita moschata OX=3662 GN=LOC111455044 PE=...	[more]
A0A6J1C1F3	6.4e-126	75.59	probable O-methyltransferase 3 OS=Momordica charantia OX=3673 GN=LOC111007572 PE...	[more]

BLAST of Sgr015334 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G35160.1	1.0e-51	39.72	O-methyltransferase family protein	[more]
AT4G35150.1	1.1e-42	36.30	O-methyltransferase family protein	[more]
AT5G54160.1	4.2e-37	34.98	O-methyltransferase 1	[more]
AT1G77520.1	4.3e-34	32.36	O-methyltransferase family protein	[more]
AT1G63140.2	4.8e-33	33.11	O-methyltransferase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012967	Plant methyltransferase dimerisation	PFAM	PF08100	Dimerisation	coord: 34..83 e-value: 1.3E-15 score: 57.2
IPR001077	O-methyltransferase domain	PFAM	PF00891	Methyltransf_2	coord: 141..298 e-value: 8.7E-46 score: 155.9
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 118..304 e-value: 7.1E-65 score: 220.5
None	No IPR available	PIRSR	PIRSR005739-1	PIRSR005739-1	coord: 33..292 e-value: 5.2E-71 score: 236.9
None	No IPR available	PANTHER	PTHR11746:SF250	O-METHYLTRANSFERASE	coord: 11..299
None	No IPR available	CDD	cd02440	AdoMet_MTases	coord: 207..299 e-value: 1.47135E-4 score: 38.5651
IPR016461	O-methyltransferase COMT-type	PIRSF	PIRSF005739	O-mtase	coord: 4..304 e-value: 2.7E-108 score: 360.0
IPR016461	O-methyltransferase COMT-type	PANTHER	PTHR11746	O-METHYLTRANSFERASE	coord: 11..299
IPR016461	O-methyltransferase COMT-type	PROSITE	PS51683	SAM_OMT_II	coord: 24..310 score: 65.066353
IPR036388	Winged helix-like DNA-binding domain superfamily	GENE3D	1.10.10.10		coord: 10..117 e-value: 1.6E-39 score: 135.6
IPR036390	Winged helix DNA-binding domain superfamily	SUPERFAMILY	46785	"Winged helix" DNA-binding domain	coord: 13..122
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 124..291

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Sgr015334.1	Sgr015334.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0019438	aromatic compound biosynthetic process
biological_process	GO:0032259	methylation
molecular_function	GO:0008171	O-methyltransferase activity
molecular_function	GO:0046983	protein dimerization activity
molecular_function	GO:0008757	S-adenosylmethionine-dependent methyltransferase activity
molecular_function	GO:0008168	methyltransferase activity