Sed0012612 (gene) Chayote v1

Overview


Name	Sed0012612
Type	gene
Organism	Sechium edule (Chayote v1)
Description	Eukaryotic aspartyl protease family protein
Location	LG01: 5804527 .. 5807193 (-)
RNA-Seq Expression	Sed0012612
Synteny	Sed0012612

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTGGAATACTTTTAATTTCTTAATCCTTCACCTCCTCATTGTCCTCTCCGTTAGCCTCGTGGCGAACGAGGTTAACGCAGGGGGACAAGAGAAGGTGAGGCTGAATATGATGCATCGCCACCATCCAAAGATAGTGGAAAGGCTCAAAATCAATATGAACGCGGAAGACATGAACGATCGTGTGAAGGACATTCACATGCACGACAGTAGTAGGCACCGCATGCTCACGGCGTTGCGGGTTCGGCAGTTAGTTGAGACGAGAGTGCAAGGATTATCGACTACGGAGCTGCCAGCACCGTTGGCGACGGGGATAGGGCTGAAGATGTTCTCTGGTGCGGACTACGGAAGCAGCGAGTATTTCGTGGAGATGAAAATCGGAACGCCGCCGCAGAAACACATGTTGATCGTCGACACGGGAAGTGATCTGTTGTGGGTCAAATGTAGGTACAGGCGCTGCATAGGAGCAAATTGCAACCATGGGAATGCGATTCATAAGAGCCGCAATGATCAAAGAGAGAAAATGGGCCGTGTGTTCTATGCTAATCGTTCATCTTCTTTTGTGGCCGTACCCTGCAGTTCAAACGAGTGTTCGGATGAATTTACACATTTCTTCGTCTTGCGTGATTGCAAAGTTCCAACCGACCCTTGTCGCTATGATTACAGGTACTCATTTTTAATAACAAAAATTTCTCTGCATATCAATCTATATAAGTGGAACTCTACATTTAGGGTGTGTTTGGGCACCCGTTATGTAAACGACCGTGTTGGTTATAATAATTCAACCAATGTTTGCCCCACAATTTATTATAAACGGTGGTTACCTAAACCTCTGTGCCCGTTTATCTAAACCTTATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATACTTTTTGTTGCATAATCAGCCCCTCAAACATCGTCTATCATAATTCATGTTATAATAACTTGCTCCTTAAACACTATTTATAATAACTCACTGTTTATTATAACTTCTGTCTATCATAACTAAACCGGTGCTCCAAACACACCCTTTATATATATTCTATTAGGTATCACATAAATTTATTTTGGAATAAAAATATAGGGAAGTTTTTTTTTTTTTTTTTTTTTTTTATTCAATCCAAACTAAAATCCATTATGATTACTTTGATTAGGATTTTACATTAATTCACCCAAAATGATAGTTTTTTTTTATATAATTATTTGTAGTTTAATATCTAAATTTGCATTAAAAAAAACATTTTAGGACCTTTTTTCAAAATTTTCTAGCCAACAATTTCAAAAGTAGGTAATAATTGAGGCGTAAAAAGAAATGAATAAAACTCTCATACATGTGAATATGGTGTCACTATGGAATGTTACAAAAATTTATCTTATAAATAAAGATATTAAAAAATATATAAATTGTTGTGGCTCCATCTATATACACGAATGTGCAGGGAATAAGAAAATTGAAAAAATAAAATTTTACGTATGTGTCGCACAAATATTTTTTATAAATAAAAAAGAAGAAAAAGAATATGTATGGCAGGAAATGTGGGACATAGGTAAAATGTAGCTAAAATTCAAAATGGGGAGAAGAAAGACATGTATTGGATGATGGAGGATGGAAACTGAAAAGGGAAAGACATTTGCAAGTGTATGGAATTTAATTATGTTAGAAAAATGGAAGAATTTAATGACTAATATGGTGTAATGTTGAATATTGAATAATATTAGTATGATAAGAAAGTATGCGAGGAGGATAGTGGTGAATAGAAATGAGTATATTTATTTGTAACAGCTATGCAGGCGGATCAAGTGCAAGGGGGCTATTTGCAGTGGAAACATTAACGGTAAACTTAACCGACGGAACAGAGAAACAACTGAACGACAGTGTAATCGGGTGCACAGAATCAGCGTCCGGCAAAGTTTTTAAGGGAGCGGACGGGGTGGTTGGATTAGGTGCAAACGTGTTCTCGTTCACCTACAAAGCCGGCCAAAACTTCCAAGGCGGTGGCTTTACGTACTGCCTCGTCGACCACCTCAGCAACCGCCTCGCAACCAGCTACTTCATCCTCGGCCCCCCGTCTCCCGCCACACGCACCGCCGCCACCACCACCGCTGTCACCGGCGCCACCACCAAGACCGCACTCCTCGCTAGCGACATGTTTCGGGGGCTCTACGGCATGGGCCTCAACGGAATCTCGGTCGACGGCACCATCCTCGACATCCCGCCTCGCGTCTGGGACACCAACAGCAGCGGCGGCACCATCATCGACACCGGCACCAGCTTGACGATGCTGACGATGCCAGCCTACGAAATGGTGTCGGAAGCTTTTCTTTCCAAATTCTCAAACCTCCCCAGGTTGAGTTTCGATCCATTTGATATCTGCTTCAATAACACCATGTACCACAACTCCATGGCCCCGAAGCTGCGGCTCCATTTCAGCGACGGCGCCGTGTTCCAGCCGCCATCCAAGAGCTACATCCTCCCCATTGCCGGGGACACTAGTTGTTTAGGGATAGTTCCGGTGCCTTTTCCGGGCACCAATGTCATTGGGAATATTCTCCAACAAAATTATCTCTGGCAATTTGATTTCAATAAAAATACCGTCACTTTTACTCCCTCTGAATGCGTCTAG

mRNA sequence

ATGTGGAATACTTTTAATTTCTTAATCCTTCACCTCCTCATTGTCCTCTCCGTTAGCCTCGTGGCGAACGAGGTTAACGCAGGGGGACAAGAGAAGGTGAGGCTGAATATGATGCATCGCCACCATCCAAAGATAGTGGAAAGGCTCAAAATCAATATGAACGCGGAAGACATGAACGATCGTGTGAAGGACATTCACATGCACGACAGTAGTAGGCACCGCATGCTCACGGCGTTGCGGGTTCGGCAGTTAGTTGAGACGAGAGTGCAAGGATTATCGACTACGGAGCTGCCAGCACCGTTGGCGACGGGGATAGGGCTGAAGATGTTCTCTGGTGCGGACTACGGAAGCAGCGAGTATTTCGTGGAGATGAAAATCGGAACGCCGCCGCAGAAACACATGTTGATCGTCGACACGGGAAGTGATCTGTTGTGGGTCAAATGTAGGTACAGGCGCTGCATAGGAGCAAATTGCAACCATGGGAATGCGATTCATAAGAGCCGCAATGATCAAAGAGAGAAAATGGGCCGTGTGTTCTATGCTAATCGTTCATCTTCTTTTGTGGCCGTACCCTGCAGTTCAAACGAGTGTTCGGATGAATTTACACATTTCTTCGTCTTGCGTGATTGCAAAGTTCCAACCGACCCTTGTCGCTATGATTACAGCTATGCAGGCGGATCAAGTGCAAGGGGGCTATTTGCAGTGGAAACATTAACGGTAAACTTAACCGACGGAACAGAGAAACAACTGAACGACAGTGTAATCGGGTGCACAGAATCAGCGTCCGGCAAAGTTTTTAAGGGAGCGGACGGGGTGGTTGGATTAGGTGCAAACGTGTTCTCGTTCACCTACAAAGCCGGCCAAAACTTCCAAGGCGGTGGCTTTACGTACTGCCTCGTCGACCACCTCAGCAACCGCCTCGCAACCAGCTACTTCATCCTCGGCCCCCCGTCTCCCGCCACACGCACCGCCGCCACCACCACCGCTGTCACCGGCGCCACCACCAAGACCGCACTCCTCGCTAGCGACATGTTTCGGGGGCTCTACGGCATGGGCCTCAACGGAATCTCGGTCGACGGCACCATCCTCGACATCCCGCCTCGCGTCTGGGACACCAACAGCAGCGGCGGCACCATCATCGACACCGGCACCAGCTTGACGATGCTGACGATGCCAGCCTACGAAATGGTGTCGGAAGCTTTTCTTTCCAAATTCTCAAACCTCCCCAGGTTGAGTTTCGATCCATTTGATATCTGCTTCAATAACACCATGTACCACAACTCCATGGCCCCGAAGCTGCGGCTCCATTTCAGCGACGGCGCCGTGTTCCAGCCGCCATCCAAGAGCTACATCCTCCCCATTGCCGGGGACACTAGTTGTTTAGGGATAGTTCCGGTGCCTTTTCCGGGCACCAATGTCATTGGGAATATTCTCCAACAAAATTATCTCTGGCAATTTGATTTCAATAAAAATACCGTCACTTTTACTCCCTCTGAATGCGTCTAG

Coding sequence (CDS)

ATGTGGAATACTTTTAATTTCTTAATCCTTCACCTCCTCATTGTCCTCTCCGTTAGCCTCGTGGCGAACGAGGTTAACGCAGGGGGACAAGAGAAGGTGAGGCTGAATATGATGCATCGCCACCATCCAAAGATAGTGGAAAGGCTCAAAATCAATATGAACGCGGAAGACATGAACGATCGTGTGAAGGACATTCACATGCACGACAGTAGTAGGCACCGCATGCTCACGGCGTTGCGGGTTCGGCAGTTAGTTGAGACGAGAGTGCAAGGATTATCGACTACGGAGCTGCCAGCACCGTTGGCGACGGGGATAGGGCTGAAGATGTTCTCTGGTGCGGACTACGGAAGCAGCGAGTATTTCGTGGAGATGAAAATCGGAACGCCGCCGCAGAAACACATGTTGATCGTCGACACGGGAAGTGATCTGTTGTGGGTCAAATGTAGGTACAGGCGCTGCATAGGAGCAAATTGCAACCATGGGAATGCGATTCATAAGAGCCGCAATGATCAAAGAGAGAAAATGGGCCGTGTGTTCTATGCTAATCGTTCATCTTCTTTTGTGGCCGTACCCTGCAGTTCAAACGAGTGTTCGGATGAATTTACACATTTCTTCGTCTTGCGTGATTGCAAAGTTCCAACCGACCCTTGTCGCTATGATTACAGCTATGCAGGCGGATCAAGTGCAAGGGGGCTATTTGCAGTGGAAACATTAACGGTAAACTTAACCGACGGAACAGAGAAACAACTGAACGACAGTGTAATCGGGTGCACAGAATCAGCGTCCGGCAAAGTTTTTAAGGGAGCGGACGGGGTGGTTGGATTAGGTGCAAACGTGTTCTCGTTCACCTACAAAGCCGGCCAAAACTTCCAAGGCGGTGGCTTTACGTACTGCCTCGTCGACCACCTCAGCAACCGCCTCGCAACCAGCTACTTCATCCTCGGCCCCCCGTCTCCCGCCACACGCACCGCCGCCACCACCACCGCTGTCACCGGCGCCACCACCAAGACCGCACTCCTCGCTAGCGACATGTTTCGGGGGCTCTACGGCATGGGCCTCAACGGAATCTCGGTCGACGGCACCATCCTCGACATCCCGCCTCGCGTCTGGGACACCAACAGCAGCGGCGGCACCATCATCGACACCGGCACCAGCTTGACGATGCTGACGATGCCAGCCTACGAAATGGTGTCGGAAGCTTTTCTTTCCAAATTCTCAAACCTCCCCAGGTTGAGTTTCGATCCATTTGATATCTGCTTCAATAACACCATGTACCACAACTCCATGGCCCCGAAGCTGCGGCTCCATTTCAGCGACGGCGCCGTGTTCCAGCCGCCATCCAAGAGCTACATCCTCCCCATTGCCGGGGACACTAGTTGTTTAGGGATAGTTCCGGTGCCTTTTCCGGGCACCAATGTCATTGGGAATATTCTCCAACAAAATTATCTCTGGCAATTTGATTTCAATAAAAATACCGTCACTTTTACTCCCTCTGAATGCGTCTAG

Protein sequence

MWNTFNFLILHLLIVLSVSLVANEVNAGGQEKVRLNMMHRHHPKIVERLKINMNAEDMNDRVKDIHMHDSSRHRMLTALRVRQLVETRVQGLSTTELPAPLATGIGLKMFSGADYGSSEYFVEMKIGTPPQKHMLIVDTGSDLLWVKCRYRRCIGANCNHGNAIHKSRNDQREKMGRVFYANRSSSFVAVPCSSNECSDEFTHFFVLRDCKVPTDPCRYDYSYAGGSSARGLFAVETLTVNLTDGTEKQLNDSVIGCTESASGKVFKGADGVVGLGANVFSFTYKAGQNFQGGGFTYCLVDHLSNRLATSYFILGPPSPATRTAATTTAVTGATTKTALLASDMFRGLYGMGLNGISVDGTILDIPPRVWDTNSSGGTIIDTGTSLTMLTMPAYEMVSEAFLSKFSNLPRLSFDPFDICFNNTMYHNSMAPKLRLHFSDGAVFQPPSKSYILPIAGDTSCLGIVPVPFPGTNVIGNILQQNYLWQFDFNKNTVTFTPSECV

Homology

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BLAST of Sed0012612 vs. NCBI nr
Match: XP_038901983.1 (aspartic proteinase NANA, chloroplast [Benincasa hispida])

HSP 1 Score: 553.1 bits (1424), Expect = 2.4e-153
Identity = 270/504 (53.57%), Postives = 358/504 (71.03%), Query Frame = 0


Query: 6   NFLILHLLIVLSVSLVANEVNAGGQEKVRLNMMHRHHPKIVERLKINMNAEDMNDRVKDI 65
           +F +  L   LSV +   +  +  QE V+L+++HRHHP++ E+L  ++  E+MNDR+KDI
Sbjct: 13  HFCLFFLFFFLSVPIAFGD-GSHDQENVKLDLLHRHHPQVSEKLHGDIKLENMNDRIKDI 72

Query: 66  HMHDSSRHRMLTALRVRQLVETRVQ------GLSTTELPAPLATGIGLKMFSGADYGSSE 125
             HD  R++ +++   R  ++ +++           +LP   +T IGLKM SG+DYGSSE
Sbjct: 73  LEHDQKRYQTISSSLNRNELDEQLRKEAAELAEKDLKLPPISSTPIGLKMISGSDYGSSE 132

Query: 126 YFVEMKIGTPPQKHMLIVDTGSDLLWVKCRYRRCIGANCNHGNAIHKSRNDQREKMGRVF 185
           YFV++K+GTPPQ  MLI DTGSDL W+KCRYRRCIG NC+  N  HK+RN+++ +    F
Sbjct: 133 YFVQLKVGTPPQTFMLIADTGSDLTWMKCRYRRCIG-NCS-SNPNHKTRNERKVRFRNAF 192

Query: 186 YANRSSSFVAVPCSSNECSDEFTHFFVLRDCKVPTDPCRYDYSYAGGSSARGLFAVETLT 245
            AN SSSF  + CSS  C+++    F + +C+ PT PC YDYSY+GG+SA+GLFA+ETLT
Sbjct: 193 LANYSSSFKTIDCSSKMCTNDLADLFSIGECQTPTSPCLYDYSYSGGASAKGLFAIETLT 252

Query: 246 VNLTDGTEKQLNDSVIGCTESASGKVFKGADGVVGLGANVFSFTYKAGQNFQGGGFTYCL 305
           V LT+G EKQL++S+IGCTES  G++F GADGV+GLG + +SFTYKA +N  GGGF YCL
Sbjct: 253 VGLTNGKEKQLHNSIIGCTESVQGRIFGGADGVIGLGTSSYSFTYKAAENANGGGFAYCL 312

Query: 306 VDHLSNRLATSYFILGPPSPATRTAATTTAV--TGATTKTALLASDMFRGLYGMGLNGIS 365
           VDHLS+R ATSYFILG P  +T +AA  ++V  TG  + T L   D +   YG+ L GIS
Sbjct: 313 VDHLSDRTATSYFILGNPISSTDSAAAASSVAPTGNMSFTKLFLGDPYSSFYGVDLVGIS 372

Query: 366 VDGTILDIPPRVWDTNSSGGTIIDTGTSLTMLTMPAYEMVSEAFLSKFSNLPRLSFDPFD 425
            DG +L+IPPRVWD NS GGTI+D+GTSLTML  PA++MV EA + K  +   +  +PFD
Sbjct: 373 ADGVMLNIPPRVWDINSGGGTIVDSGTSLTMLAAPAFDMVMEALVPKLKHFENIEIEPFD 432

Query: 426 ICFNNTMYHNSMAPKLRLHFSDGAVFQPPSKSYILPIAGDTSCLGIVPVPFPGTNVIGNI 485
            CFNN+ Y + MAPKLR HF DG VFQPP KSYI+ +    SC+G V +PFP TN+IGNI
Sbjct: 433 FCFNNSRYTHEMAPKLRFHFGDGTVFQPPPKSYIVSVGEYISCIGFVSMPFPATNIIGNI 492

Query: 486 LQQNYLWQFDFNKNTVTFTPSECV 502
           LQQN+LW+FDF+  TV F PSECV
Sbjct: 493 LQQNHLWKFDFHAGTVGFAPSECV 513

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BLAST of Sed0012612 vs. NCBI nr
Match: XP_004140022.2 (aspartic proteinase NANA, chloroplast [Cucumis sativus] >KGN46781.1 hypothetical protein Csa_021058 [Cucumis sativus])

HSP 1 Score: 534.3 bits (1375), Expect = 1.2e-147
Identity = 254/483 (52.59%), Postives = 340/483 (70.39%), Query Frame = 0


Query: 30  QEKVRLNMMHRHHPKIVERLKINMNAEDMNDRVKDIHMHDSSRHRMLTALRVRQLVE--- 89
           QE ++ +++HRHHP++ E++  +M  +D+++R+KDIH HD +RHR ++    ++ VE   
Sbjct: 57  QEIIKFDLLHRHHPQVAEKIHGDMKIQDVSERMKDIHEHDHNRHRSISKSMNQKQVEDAR 116

Query: 90  TRVQGLSTTE--------LPAPLATGIGLKMFSGADYGSSEYFVEMKIGTPPQKHMLIVD 149
            R +  + TE        LP   +T IG++M SGAD+GSSEYFVE+K+GTP Q  MLI D
Sbjct: 117 LRAEAEAATEEEVAKSAILPPATSTPIGMRMISGADFGSSEYFVELKVGTPAQTFMLIAD 176

Query: 150 TGSDLLWVKCRYRRCIGANCNHGNAIHKSRNDQREKMGRVFYANRSSSFVAVPCSSNECS 209
           TGSDL W+KCRYRRC G NC+  N  HKS+N+++++    F AN SSSF  V CSS  C+
Sbjct: 177 TGSDLTWMKCRYRRCFG-NCS-SNVNHKSKNEKKQRFRHAFLANHSSSFKTVSCSSTMCT 236

Query: 210 DEFTHFFVLRDCKVPTDPCRYDYSYAGGSSARGLFAVETLTVNLTDGTEKQLNDSVIGCT 269
           ++    F +R+C  PT PC YDYSY GG+SA+G+FA ETLTV LT+G EKQL++S+IGCT
Sbjct: 237 NDLADLFAVRECHNPTSPCVYDYSYTGGASAKGIFAWETLTVGLTNGKEKQLHNSIIGCT 296

Query: 270 ESASGKVFKGADGVVGLGANVFSFTYKAGQNFQGGGFTYCLVDHLSNRLATSYFILGPPS 329
           ES  G VF GADGV+GLG + +S TYKA +N  GGGF+YCLVDHL+++ A SYF+LG P+
Sbjct: 297 ESVQGSVFGGADGVMGLGTSSYSLTYKAAENANGGGFSYCLVDHLTDQRAISYFVLGIPT 356

Query: 330 PATRTAATTTAVTGATTKTALLASDMFRGLYGMGLNGISVDGTILDIPPRVWDTNSSGGT 389
           P+T  + ++  +    T T L   D +   YG+ L GIS +G +L+IP RVWD NS GGT
Sbjct: 357 PSTSASTSSAKLPAKMTYTKLYVGDPYSSFYGVDLIGISANGIMLNIPSRVWDINSGGGT 416

Query: 390 IIDTGTSLTMLTMPAYEMVSEAFLSKFSNLPRLSFDPFDICFNNTMYHNSMAPKLRLHFS 449
           IID+GTSLT+L  PA++MV EA   +     +L  +PFD CFNN+ Y + MAPKLR HF 
Sbjct: 417 IIDSGTSLTILAAPAFDMVMEALTPRLKKFQQLEIEPFDFCFNNSQYTHEMAPKLRFHFG 476

Query: 450 DGAVFQPPSKSYILPIAGDTSCLGIVPVPFPGTNVIGNILQQNYLWQFDFNKNTVTFTPS 502
           DG VF+PP+KSYI+ +    SC+G V +PFP  N+IGNILQQN+LWQFDF K  V F PS
Sbjct: 477 DGTVFEPPTKSYIVSVGKFISCIGFVSMPFPANNIIGNILQQNHLWQFDFQKRRVGFAPS 536

[Close]

BLAST of Sed0012612 vs. NCBI nr
Match: XP_008456273.1 (PREDICTED: aspartic proteinase CDR1 [Cucumis melo])

HSP 1 Score: 523.5 bits (1347), Expect = 2.0e-144
Identity = 256/517 (49.52%), Postives = 345/517 (66.73%), Query Frame = 0


Query: 5   FNFLILHLLIVLSVSLVA---------NEVNAGGQEKVRLNMMHRHHPKIVERLKINMNA 64
           F FL+L  L   S  L A         N  +   Q+ +R +++HRHHP++ E+L  +M  
Sbjct: 16  FFFLLLFFLSFSSSFLFALGDEANNYNNNDDEDEQQTIRFDLLHRHHPQVSEKLNGDMKI 75

Query: 65  EDMNDRVKDIHMHDSSRHRMLT-----------ALRVRQLVETRVQGLSTTELPAPLATG 124
           +D+++R+KDIH HD +RHR ++            LR      T+V+   +  LP   +T 
Sbjct: 76  QDLHERMKDIHEHDRNRHRSISKSMNQKQIEDARLRAEAEAATQVEVAKSAILPPATSTP 135

Query: 125 IGLKMFSGADYGSSEYFVEMKIGTPPQKHMLIVDTGSDLLWVKCRYRRCIGANCNHGNAI 184
           IG+KM SGAD+GSSEYFV++K+GTP Q  MLI DTGSDL W+KCRYRRC G NC+ GN  
Sbjct: 136 IGMKMISGADFGSSEYFVQLKVGTPAQTFMLIADTGSDLTWLKCRYRRCFG-NCS-GNVN 195

Query: 185 HKSRNDQREKMGRVFYANRSSSFVAVPCSSNECSDEFTHFFVLRDCKVPTDPCRYDYSYA 244
           HKS+N+++++      AN+SS+F  V CSS  C++     F + +C  PT PC YDYSYA
Sbjct: 196 HKSKNEKKQRFRHALLANQSSTFKTVSCSSTMCTNNLAELFAVAECDTPTSPCVYDYSYA 255

Query: 245 GGSSARGLFAVETLTVNLTDGTEKQLNDSVIGCTESASGKVFKGADGVVGLGANVFSFTY 304
           GG+SA+G+FA ETLTV LT+G EKQL +S+IGCTE   G VF GADGV+GLG + +S TY
Sbjct: 256 GGASAKGIFAWETLTVGLTNGKEKQLRNSIIGCTEIVQGNVFDGADGVMGLGTSSYSLTY 315

Query: 305 KAGQNFQGGGFTYCLVDHLSNRLATSYFILGPPSPATRTAATTTAVTGATTKTALLASDM 364
           KA +N  GGGF+YCLVDHL+++ A SYF+LG P+P+T  + ++       + T L   D 
Sbjct: 316 KAAENANGGGFSYCLVDHLTDQRAVSYFVLGVPTPSTSASTSSAKPPAKMSYTKLYVGDP 375

Query: 365 FRGLYGMGLNGISVDGTILDIPPRVWDTNSSGGTIIDTGTSLTMLTMPAYEMVSEAFLSK 424
           +   YG+ L GIS DG +L+IPPRVWD+    GTIID+GTSLT+L  PA+++V E   S+
Sbjct: 376 YSSFYGVDLIGISADGQMLNIPPRVWDSYKGCGTIIDSGTSLTVLATPAFDVVMEVLTSR 435

Query: 425 FSNLPRLSFDPFDICFNNTMYHNSMAPKLRLHFSDGAVFQPPSKSYILPIAGDTSCLGIV 484
                ++  +PF+ CFNN+ Y + MAPKLR HF DG VF+PP+KSYI+ +    SC+GIV
Sbjct: 436 LKQFQQIEIEPFNFCFNNSQYTHDMAPKLRFHFGDGTVFEPPTKSYIVSVGEFISCIGIV 495

Query: 485 PVPFPGTNVIGNILQQNYLWQFDFNKNTVTFTPSECV 502
            +PFP  N+IGNILQQN+LWQFDF K  V F  SEC+
Sbjct: 496 SMPFPSLNIIGNILQQNHLWQFDFQKRRVGFAASECI 530

[Close]

BLAST of Sed0012612 vs. NCBI nr
Match: KAA0033565.1 (aspartic proteinase CDR1 [Cucumis melo var. makuwa] >TYJ95622.1 aspartic proteinase CDR1 [Cucumis melo var. makuwa])

HSP 1 Score: 500.7 bits (1288), Expect = 1.4e-137
Identity = 238/460 (51.74%), Postives = 316/460 (68.70%), Query Frame = 0


Query: 53  MNAEDMNDRVKDIHMHDSSRHRMLT-----------ALRVRQLVETRVQGLSTTELPAPL 112
           M  +D+++R+KDIH HD +RHR ++            LR      T+V+   +  LP   
Sbjct: 1   MKIQDLHERMKDIHEHDRNRHRSISKSMNQKQIEDARLRAEAEAATQVEVAKSAILPPAT 60

Query: 113 ATGIGLKMFSGADYGSSEYFVEMKIGTPPQKHMLIVDTGSDLLWVKCRYRRCIGANCNHG 172
           +T IG+KM SGAD+GSSEYFV++K+GTP Q  MLI DTGSDL W+KCRYRRC G NC+ G
Sbjct: 61  STPIGMKMISGADFGSSEYFVQLKVGTPAQTFMLIADTGSDLTWLKCRYRRCFG-NCS-G 120

Query: 173 NAIHKSRNDQREKMGRVFYANRSSSFVAVPCSSNECSDEFTHFFVLRDCKVPTDPCRYDY 232
           N  HKS+N+++++      AN+SS+F  V CSS  C++     F + +C  PT PC YDY
Sbjct: 121 NVNHKSKNEKKQRFRHALLANQSSTFKTVSCSSTMCTNNLAELFAVAECDTPTSPCVYDY 180

Query: 233 SYAGGSSARGLFAVETLTVNLTDGTEKQLNDSVIGCTESASGKVFKGADGVVGLGANVFS 292
           SYAGG+SA+G+FA ETLTV LT+G EKQL +S+IGCTE   G VF GADGV+GLG + +S
Sbjct: 181 SYAGGASAKGIFAWETLTVGLTNGKEKQLRNSIIGCTEIVQGNVFDGADGVMGLGTSSYS 240

Query: 293 FTYKAGQNFQGGGFTYCLVDHLSNRLATSYFILGPPSPATRTAATTTAVTGATTKTALLA 352
            TYKA +N  GGGF+YCLVDHL+++ A SYF+LG P+P+T  + ++       + T L  
Sbjct: 241 LTYKAAENANGGGFSYCLVDHLTDQRAVSYFVLGVPTPSTSASTSSAKPPAKMSYTKLYV 300

Query: 353 SDMFRGLYGMGLNGISVDGTILDIPPRVWDTNSSGGTIIDTGTSLTMLTMPAYEMVSEAF 412
            D +   YG+ L GIS DG +L+IPPRVWD+    GTIID+GTSLT+L  PA+++V E  
Sbjct: 301 GDPYSSFYGVDLIGISADGQMLNIPPRVWDSYKGCGTIIDSGTSLTVLATPAFDVVMEVL 360

Query: 413 LSKFSNLPRLSFDPFDICFNNTMYHNSMAPKLRLHFSDGAVFQPPSKSYILPIAGDTSCL 472
            S+     ++  +PF+ CFNN+ Y + MAPKLR HF DG VF+PP+KSYI+ +    SC+
Sbjct: 361 TSRLKQFQQIEIEPFNFCFNNSQYTHDMAPKLRFHFGDGTVFEPPTKSYIVSVGEFISCI 420

Query: 473 GIVPVPFPGTNVIGNILQQNYLWQFDFNKNTVTFTPSECV 502
           GIV +PFP  N+IGNILQQN+LWQFDF K  V F  SEC+
Sbjct: 421 GIVSMPFPSLNIIGNILQQNHLWQFDFQKRRVGFAASECI 458

[Close]

BLAST of Sed0012612 vs. NCBI nr
Match: XP_023533886.1 (aspartic proteinase NANA, chloroplast-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 478.0 bits (1229), Expect = 9.9e-131
Identity = 238/491 (48.47%), Postives = 315/491 (64.15%), Query Frame = 0


Query: 23  NEVNAGGQEKVRLNMMHRHHPKIVERLKINMNAEDMNDRVKDIHMHDSSRHRMLTA---- 82
           ++ N   QE VRL+++HRHHP++V+RL   +  + M DR+KDI  HD SR R ++A    
Sbjct: 35  SDANNEEQEIVRLDLIHRHHPEVVKRLHDEIKVDKMEDRIKDIRYHDQSRLRAISAHLNW 94

Query: 83  -LRVRQLVETRVQGLSTTELPAPLATGIGLKMFSGADYGSSEYFVEMKIGTPPQKHMLIV 142
              V    E   +  S + LP    + I LK + GADYGSSE+FV++K+GTPPQK  +I 
Sbjct: 95  TKVVENAEEKEKKEASGSNLPPQSQSPIALKTYPGADYGSSEFFVQLKLGTPPQKFTMIA 154

Query: 143 DTGSDLLWVKCRYRRCIGANCNHGNAIHKSRNDQREKMGRVFYANRSSSFVAVPCSSNEC 202
           DTGSDLLW +CRYRRC G +C++ + IHK RN  R++     YAN+SSSF  +PCSS +C
Sbjct: 155 DTGSDLLWTRCRYRRCRG-DCSNPSPIHKMRNRMRDRFIYALYANQSSSFSPIPCSSEQC 214

Query: 203 SDEFTHFFVLRDCKVPTDPCRYDYSYAGGSSARGLFAVETLTVNLTDGTEKQLNDSVIGC 262
             +F+      DC  P  PC Y YSY  G  A G+FA ET+TV LT+G EKQL D + GC
Sbjct: 215 IQDFSELGGQPDCPTPNSPCSYTYSYLSGDCAMGIFATETVTVRLTNGKEKQLKDILYGC 274

Query: 263 TES-ASGKVFKGADGVVGLGANVFSFTYKAGQNFQGGGFTYCLVDHLSNRLATSYFILGP 322
           TE     K   GADG++GLG++++SF YKA +N  GGGF+YCL DH+ +  A SYF+ G 
Sbjct: 275 TEEITDSKFLDGADGLIGLGSSIYSFVYKAAENNIGGGFSYCLADHIRSITAISYFVFGT 334

Query: 323 PSPATRTAATTTAVTGATTKTALLASDMFRGLYGMGLNGISVDGTILDIPPRVWDTNSSG 382
           PSP T  A+T+T + G    T L+    +   YG+ L GISVDG IL+IPP VW+ NS  
Sbjct: 335 PSPKTFAASTSTPI-GPPATTKLITGGRYSCYYGVQLAGISVDGQILNIPPHVWNINSGC 394

Query: 383 GTIIDTGTSLTMLTMPAYEMVSEAFLSKFSNLPRLSFD-------PFDICFNNTMYHNSM 442
           GTI+DTGTSLTMLT PA++ V EA   K     R+  D        F +CFN+T +   M
Sbjct: 395 GTILDTGTSLTMLTAPAHDAVIEAMAPKIEKFGRMERDVKGEREKNFKLCFNDTEWRFGM 454

Query: 443 APKLRLHFSDGAVFQPPSKSYILPIAGDTSCLGIVPVPFPGTNVIGNILQQNYLWQFDFN 501
            PKL  HF  G VF+PP +SY++P +   SC+ I  +PFP  N++GNI+QQ YLWQFD +
Sbjct: 455 TPKLGFHFEGGVVFEPPDRSYVVPASEQCSCIAITSLPFPSINILGNIIQQTYLWQFDLH 514

[Close]

BLAST of Sed0012612 vs. ExPASy Swiss-Prot
Match: Q9LTW4 (Aspartic proteinase NANA, chloroplast OS=Arabidopsis thaliana OX=3702 GN=NANA PE=1 SV=1)

HSP 1 Score: 303.9 bits (777), Expect = 3.3e-81
Identity = 176/443 (39.73%), Postives = 242/443 (54.63%), Query Frame = 0


Query: 61  RVKDIHMHDSSRHRMLTALRVRQLVETRVQGLSTTELPAPLATGIGLKMFSGADYGSSEY 120
           R++D+   D  RH +++  R                       G+ + + SG DYG+++Y
Sbjct: 66  RIEDVIGADQKRHSLISRKR-------------------NSTVGVKMDLGSGIDYGTAQY 125

Query: 121 FVEMKIGTPPQKHMLIVDTGSDLLWVKCRYRRCIGANCNHGNAIHKSRNDQREKMGRVFY 180
           F E+++GTP +K  ++VDTGS+L WV CRYR                  D R    RVF 
Sbjct: 126 FTEIRVGTPAKKFRVVVDTGSELTWVNCRYR--------------ARGKDNR----RVFR 185

Query: 181 ANRSSSFVAVPCSSNECSDEFTHFFVLRDCKVPTDPCRYDYSYAGGSSARGLFAVETLTV 240
           A+ S SF  V C +  C  +  + F L  C  P+ PC YDY YA GS+A+G+FA ET+TV
Sbjct: 186 ADESKSFKTVGCLTQTCKVDLMNLFSLTTCPTPSTPCSYDYRYADGSAAQGVFAKETITV 245

Query: 241 NLTDGTEKQLNDSVIGCTESASGKVFKGADGVVGLGANVFSFTYKAGQNFQGGGFTYCLV 300
            LT+G   +L   +IGC+ S +G+ F+GADGV+GL  + FSFT  A  +  G  F+YCLV
Sbjct: 246 GLTNGRMARLPGHLIGCSSSFTGQSFQGADGVLGLAFSDFSFTSTA-TSLYGAKFSYCLV 305

Query: 301 DHLSNRLATSYFILGPPSPATRTAATTTAVTGATTKTALLASDMFRGLYGMGLNGISVDG 360
           DHLSN+  ++Y I G  S +T+TA   T     T              Y + + GIS+  
Sbjct: 306 DHLSNKNVSNYLIFG-SSRSTKTAFRRTTPLDLT---------RIPPFYAINVIGISLGY 365

Query: 361 TILDIPPRVWDTNSSGGTIIDTGTSLTMLTMPAYEMVSEAFLSKFSNLPRLSFD--PFDI 420
            +LDIP +VWD  S GGTI+D+GTSLT+L   AY+ V          L R+  +  P + 
Sbjct: 366 DMLDIPSQVWDATSGGGTILDSGTSLTLLADAAYKQVVTGLARYLVELKRVKPEGVPIEY 425

Query: 421 CFNNTMYHN-SMAPKLRLHFSDGAVFQPPSKSYILPIAGDTSCLGIVPVPFPGTNVIGNI 480
           CF+ T   N S  P+L  H   GA F+P  KSY++  A    CLG V    P TNVIGNI
Sbjct: 426 CFSFTSGFNVSKLPQLTFHLKGGARFEPHRKSYLVDAAPGVKCLGFVSAGTPATNVIGNI 460

Query: 481 LQQNYLWQFDFNKNTVTFTPSEC 501
           +QQNYLW+FD   +T++F PS C
Sbjct: 486 MQQNYLWEFDLMASTLSFAPSAC 460

[Close]

BLAST of Sed0012612 vs. ExPASy Swiss-Prot
Match: Q9LS40 (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASPG1 PE=1 SV=1)

HSP 1 Score: 169.5 bits (428), Expect = 9.8e-41
Identity = 116/395 (29.37%), Postives = 187/395 (47.34%), Query Frame = 0


Query: 111 SGADYGSSEYFVEMKIGTPPQKHMLIVDTGSDLLWVKCRYRRCIGANCNHGNAIHKSRND 170
           SGA  GS EYF  + +GTP ++  L++DTGSD+ W++C    C  A+C            
Sbjct: 153 SGASQGSGEYFSRIGVGTPAKEMYLVLDTGSDVNWIQC--EPC--ADC------------ 212

Query: 171 QREKMGRVFYANRSSSFVAVPCSSNECSDEFTHFFVLRDCKVPTDPCRYDYSYAGGSSAR 230
             ++   VF    SS++ ++ CS+ +CS       +L      ++ C Y  SY  GS   
Sbjct: 213 -YQQSDPVFNPTSSSTYKSLTCSAPQCS-------LLETSACRSNKCLYQVSYGDGSFTV 272

Query: 231 GLFAVETLTVNLTDGTEKQLNDSVIGCTESASGKVFKGADGVVGLGANVFSFTYKAGQNF 290
           G  A +T+T     G   ++N+  +GC     G +F GA G++GLG  V S T       
Sbjct: 273 GELATDTVTF----GNSGKINNVALGCGHDNEG-LFTGAAGLLGLGGGVLSIT----NQM 332

Query: 291 QGGGFTYCLVDHLSNRLATSYFILGPPSPATRTAATTTAVTGATTKTALLASDMFRGLYG 350
           +   F+YCLVD  S + ++  F              +  + G      LL +      Y 
Sbjct: 333 KATSFSYCLVDRDSGKSSSLDF-------------NSVQLGGGDATAPLLRNKKIDTFYY 392

Query: 351 MGLNGISVDGTILDIPPRVWDTNS--SGGTIIDTGTSLTMLTMPAYEMVSEAFLSKFSNL 410
           +GL+G SV G  + +P  ++D ++  SGG I+D GT++T L   AY  + +AFL    NL
Sbjct: 393 VGLSGFSVGGEKVVLPDAIFDVDASGSGGVILDCGTAVTRLQTQAYNSLRDAFLKLTVNL 452

Query: 411 PR--LSFDPFDICFNNTMYHNSMAPKLRLHFSDGAVFQPPSKSYILPI-AGDTSCLGIVP 470
            +   S   FD C++ +       P +  HF+ G     P+K+Y++P+    T C    P
Sbjct: 453 KKGSSSISLFDTCYDFSSLSTVKVPTVAFHFTGGKSLDLPAKNYLIPVDDSGTFCFAFAP 500

Query: 471 VPFPGTNVIGNILQQNYLWQFDFNKNTVTFTPSEC 501
                 ++IGN+ QQ     +D +KN +  + ++C
Sbjct: 513 TS-SSLSIIGNVQQQGTRITYDLSKNVIGLSGNKC 500

[Close]

BLAST of Sed0012612 vs. ExPASy Swiss-Prot
Match: Q9LHE3 (Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASPG2 PE=2 SV=1)

HSP 1 Score: 165.2 bits (417), Expect = 1.9e-39
Identity = 116/401 (28.93%), Postives = 191/401 (47.63%), Query Frame = 0


Query: 106 GLKMFSGADYGSSEYFVEMKIGTPPQKHMLIVDTGSDLLWVKCR-YRRCIGANCNHGNAI 165
           G  + SG D GS EYFV + +G+PP+   +++D+GSD++WV+C+  + C           
Sbjct: 117 GSDIVSGMDQGSGEYFVRIGVGSPPRDQYMVIDSGSDMVWVQCQPCKLCY---------- 176

Query: 166 HKSRNDQREKMGRVFYANRSSSFVAVPCSSNECSDEFTHFFVLRDCKVPTDPCRYDYSYA 225
                   ++   VF   +S S+  V C S+ C         + +    +  CRY+  Y 
Sbjct: 177 --------KQSDPVFDPAKSGSYTGVSCGSSVCDR-------IENSGCHSGGCRYEVMYG 236

Query: 226 GGSSARGLFAVETLTVNLTDGTEKQLNDSVIGCTESASGKVFKGADGVVGLGANVFSFTY 285
            GS  +G  A+ETLT   T      + +  +GC     G +F GA G++G+G    SF  
Sbjct: 237 DGSYTKGTLALETLTFAKT-----VVRNVAMGCGHRNRG-MFIGAAGLLGIGGGSMSFVG 296

Query: 286 K-AGQNFQGGGFTYCLVDHLSNRLATSYFILGPPSPATRTAATTTAVTGATTKTALLASD 345
           + +GQ   GG F YCLV   ++  +T   + G             A+    +   L+ + 
Sbjct: 297 QLSGQT--GGAFGYCLVSRGTD--STGSLVFG-----------REALPVGASWVPLVRNP 356

Query: 346 MFRGLYGMGLNGISVDGTILDIPPRVWDTNSS--GGTIIDTGTSLTMLTMPAYEMVSEAF 405
                Y +GL G+ V G  + +P  V+D   +  GG ++DTGT++T L   AY    + F
Sbjct: 357 RAPSFYYVGLKGLGVGGVRIPLPDGVFDLTETGDGGVVMDTGTAVTRLPTAAYVAFRDGF 416

Query: 406 LSKFSNLPRLS-FDPFDICFNNTMYHNSMAPKLRLHFSDGAVFQPPSKSYILPI-AGDTS 465
            S+ +NLPR S    FD C++ + + +   P +  +F++G V   P++++++P+    T 
Sbjct: 417 KSQTANLPRASGVSIFDTCYDLSGFVSVRVPTVSFYFTEGPVLTLPARNFLMPVDDSGTY 470

Query: 466 CLGIVPVPFPGTNVIGNILQQNYLWQFDFNKNTVTFTPSEC 501
           C      P  G ++IGNI Q+     FD     V F P+ C
Sbjct: 477 CFAFAASP-TGLSIIGNIQQEGIQVSFDGANGFVGFGPNVC 470

[Close]

BLAST of Sed0012612 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 164.1 bits (414), Expect = 4.1e-39
Identity = 141/494 (28.54%), Postives = 218/494 (44.13%), Query Frame = 0


Query: 17  SVSLVANEVNAGGQEK----VRLNMMHRHHPKIVERLKINMNAEDM-NDRVKDIHMHDSS 76
           S SL+ +E  +G   +    + LN+ H      ++ L  N   +++ + R++     DS 
Sbjct: 52  SESLLESEFESGSDSESSSSITLNLDH------IDALSSNKTPDELFSSRLQ----RDSR 111

Query: 77  RHRMLTALRVRQLVETRVQGLSTTELPAPLATGIGLKMFSGADYGSSEYFVEMKIGTPPQ 136
           R + +  L        ++ G + T  P P   G    + SG   GS EYF  + +GTP +
Sbjct: 112 RVKSIATL------AAQIPGRNVTHAPRP--GGFSSSVVSGLSQGSGEYFTRLGVGTPAR 171

Query: 137 KHMLIVDTGSDLLWVKCR-YRRCIGANCNHGNAIHKSRNDQREKMGRVFYANRSSSFVAV 196
              +++DTGSD++W++C   RRC             S++D       +F   +S ++  +
Sbjct: 172 YVYMVLDTGSDIVWLQCAPCRRCY------------SQSDP------IFDPRKSKTYATI 231

Query: 197 PCSSNECSDEFTHFFVLRDCKVPTDPCRYDYSYAGGSSARGLFAVETLTVNLTDGTEKQL 256
           PCSS  C            C      C Y  SY  GS   G F+ ETLT         ++
Sbjct: 232 PCSSPHC-----RRLDSAGCNTRRKTCLYQVSYGDGSFTVGDFSTETLTFR-----RNRV 291

Query: 257 NDSVIGCTESASGKVFKGADGVVGLGANVFSFTYKAGQNFQGGGFTYCLVDHLSNRLATS 316
               +GC     G +F GA G++GLG    SF  + G  F    F+YCLVD  ++     
Sbjct: 292 KGVALGCGHDNEG-LFVGAAGLLGLGKGKLSFPGQTGHRF-NQKFSYCLVDRSAS----- 351

Query: 317 YFILGPPSPATRTAATTTAVTGATTKTALLASDMFRGLYGMGLNGISVDGT-ILDIPPRV 376
                  S  +       AV+     T LL++      Y +GL GISV GT +  +   +
Sbjct: 352 -------SKPSSVVFGNAAVSRIARFTPLLSNPKLDTFYYVGLLGISVGGTRVPGVTASL 411

Query: 377 W--DTNSSGGTIIDTGTSLTMLTMPAYEMVSEAFLSKFSNLPRL-SFDPFDICFNNTMYH 436
           +  D   +GG IID+GTS+T L  PAY  + +AF      L R   F  FD CF+ +  +
Sbjct: 412 FKLDQIGNGGVIIDSGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFDTCFDLSNMN 471

Query: 437 NSMAPKLRLHFSDGAVFQPPSKSYILPIAGDTSCLGIVPVPFPGTNVIGNILQQNYLWQF 496
               P + LHF  GA    P+ +Y++P+  +            G ++IGNI QQ +   +
Sbjct: 472 EVKVPTVVLHFR-GADVSLPATNYLIPVDTNGKFCFAFAGTMGGLSIIGNIQQQGFRVVY 484

Query: 497 DFNKNTVTFTPSEC 501
           D   + V F P  C
Sbjct: 532 DLASSRVGFAPGGC 484

[Close]

BLAST of Sed0012612 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 149.4 bits (376), Expect = 1.1e-34
Identity = 118/392 (30.10%), Postives = 171/392 (43.62%), Query Frame = 0


Query: 116 GSSEYFVEMKIGTPPQKHMLIVDTGSDLLWVKCR-YRRCIGANCNHGNAIHKSRNDQREK 175
           G  EY + + IGTP Q    I+DTGSDL+W +C+   +C   +                 
Sbjct: 91  GDGEYLMNLSIGTPAQPFSAIMDTGSDLIWTQCQPCTQCFNQST---------------- 150

Query: 176 MGRVFYANRSSSFVAVPCSSNECSDEFTHFFVLRDCKVPTDPCRYDYSYAGGSSARGLFA 235
              +F    SSSF  +PCSS  C         L       + C+Y Y Y  GS  +G   
Sbjct: 151 --PIFNPQGSSSFSTLPCSSQLCQ-------ALSSPTCSNNFCQYTYGYGDGSETQGSMG 210

Query: 236 VETLTVNLTDGTEKQLNDSVIGCTESASGKVFKGADGVVGLGANVFSFTYKAGQNFQGGG 295
            ETLT          + +   GC E+  G       G+VG+G    S             
Sbjct: 211 TETLTFG-----SVSIPNITFGCGENNQGFGQGNGAGLVGMGRGPLSLP----SQLDVTK 270

Query: 296 FTYCLVDHLSNRLATSYFILGPPSPATRTAATTTAVTGATTKTALLASDMFRGLYGMGLN 355
           F+YC+    S+    S  +LG         +   +VT  +  T L+ S      Y + LN
Sbjct: 271 FSYCMTPIGSS--TPSNLLLG---------SLANSVTAGSPNTTLIQSSQIPTFYYITLN 330

Query: 356 GISVDGTILDIPPRVWDTNS---SGGTIIDTGTSLTMLTMPAYEMVSEAFLSKFSNLPRL 415
           G+SV  T L I P  +  NS   +GG IID+GT+LT     AY+ V + F+S+  NLP +
Sbjct: 331 GLSVGSTRLPIDPSAFALNSNNGTGGIIIDSGTTLTYFVNNAYQSVRQEFISQI-NLPVV 390

Query: 416 --SFDPFDICFNN-TMYHNSMAPKLRLHFSDGAVFQPPSKSYILPIAGDTSCLGIVPVPF 475
             S   FD+CF   +   N   P   +HF DG   + PS++Y +  +    CL  +    
Sbjct: 391 NGSSSGFDLCFQTPSDPSNLQIPTFVMHF-DGGDLELPSENYFISPSNGLICLA-MGSSS 434

Query: 476 PGTNVIGNILQQNYLWQFDFNKNTVTFTPSEC 501
            G ++ GNI QQN L  +D   + V+F  ++C
Sbjct: 451 QGMSIFGNIQQQNMLVVYDTGNSVVSFASAQC 434

[Close]

BLAST of Sed0012612 vs. ExPASy TrEMBL
Match: A0A0A0KG92 (Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G134390 PE=3 SV=1)

HSP 1 Score: 534.3 bits (1375), Expect = 5.6e-148
Identity = 254/483 (52.59%), Postives = 340/483 (70.39%), Query Frame = 0


Query: 30  QEKVRLNMMHRHHPKIVERLKINMNAEDMNDRVKDIHMHDSSRHRMLTALRVRQLVE--- 89
           QE ++ +++HRHHP++ E++  +M  +D+++R+KDIH HD +RHR ++    ++ VE   
Sbjct: 57  QEIIKFDLLHRHHPQVAEKIHGDMKIQDVSERMKDIHEHDHNRHRSISKSMNQKQVEDAR 116

Query: 90  TRVQGLSTTE--------LPAPLATGIGLKMFSGADYGSSEYFVEMKIGTPPQKHMLIVD 149
            R +  + TE        LP   +T IG++M SGAD+GSSEYFVE+K+GTP Q  MLI D
Sbjct: 117 LRAEAEAATEEEVAKSAILPPATSTPIGMRMISGADFGSSEYFVELKVGTPAQTFMLIAD 176

Query: 150 TGSDLLWVKCRYRRCIGANCNHGNAIHKSRNDQREKMGRVFYANRSSSFVAVPCSSNECS 209
           TGSDL W+KCRYRRC G NC+  N  HKS+N+++++    F AN SSSF  V CSS  C+
Sbjct: 177 TGSDLTWMKCRYRRCFG-NCS-SNVNHKSKNEKKQRFRHAFLANHSSSFKTVSCSSTMCT 236

Query: 210 DEFTHFFVLRDCKVPTDPCRYDYSYAGGSSARGLFAVETLTVNLTDGTEKQLNDSVIGCT 269
           ++    F +R+C  PT PC YDYSY GG+SA+G+FA ETLTV LT+G EKQL++S+IGCT
Sbjct: 237 NDLADLFAVRECHNPTSPCVYDYSYTGGASAKGIFAWETLTVGLTNGKEKQLHNSIIGCT 296

Query: 270 ESASGKVFKGADGVVGLGANVFSFTYKAGQNFQGGGFTYCLVDHLSNRLATSYFILGPPS 329
           ES  G VF GADGV+GLG + +S TYKA +N  GGGF+YCLVDHL+++ A SYF+LG P+
Sbjct: 297 ESVQGSVFGGADGVMGLGTSSYSLTYKAAENANGGGFSYCLVDHLTDQRAISYFVLGIPT 356

Query: 330 PATRTAATTTAVTGATTKTALLASDMFRGLYGMGLNGISVDGTILDIPPRVWDTNSSGGT 389
           P+T  + ++  +    T T L   D +   YG+ L GIS +G +L+IP RVWD NS GGT
Sbjct: 357 PSTSASTSSAKLPAKMTYTKLYVGDPYSSFYGVDLIGISANGIMLNIPSRVWDINSGGGT 416

Query: 390 IIDTGTSLTMLTMPAYEMVSEAFLSKFSNLPRLSFDPFDICFNNTMYHNSMAPKLRLHFS 449
           IID+GTSLT+L  PA++MV EA   +     +L  +PFD CFNN+ Y + MAPKLR HF 
Sbjct: 417 IIDSGTSLTILAAPAFDMVMEALTPRLKKFQQLEIEPFDFCFNNSQYTHEMAPKLRFHFG 476

Query: 450 DGAVFQPPSKSYILPIAGDTSCLGIVPVPFPGTNVIGNILQQNYLWQFDFNKNTVTFTPS 502
           DG VF+PP+KSYI+ +    SC+G V +PFP  N+IGNILQQN+LWQFDF K  V F PS
Sbjct: 477 DGTVFEPPTKSYIVSVGKFISCIGFVSMPFPANNIIGNILQQNHLWQFDFQKRRVGFAPS 536

[Close]

BLAST of Sed0012612 vs. ExPASy TrEMBL
Match: A0A1S3C2F3 (aspartic proteinase CDR1 OS=Cucumis melo OX=3656 GN=LOC103496268 PE=3 SV=1)

HSP 1 Score: 523.5 bits (1347), Expect = 9.9e-145
Identity = 256/517 (49.52%), Postives = 345/517 (66.73%), Query Frame = 0


Query: 5   FNFLILHLLIVLSVSLVA---------NEVNAGGQEKVRLNMMHRHHPKIVERLKINMNA 64
           F FL+L  L   S  L A         N  +   Q+ +R +++HRHHP++ E+L  +M  
Sbjct: 16  FFFLLLFFLSFSSSFLFALGDEANNYNNNDDEDEQQTIRFDLLHRHHPQVSEKLNGDMKI 75

Query: 65  EDMNDRVKDIHMHDSSRHRMLT-----------ALRVRQLVETRVQGLSTTELPAPLATG 124
           +D+++R+KDIH HD +RHR ++            LR      T+V+   +  LP   +T 
Sbjct: 76  QDLHERMKDIHEHDRNRHRSISKSMNQKQIEDARLRAEAEAATQVEVAKSAILPPATSTP 135

Query: 125 IGLKMFSGADYGSSEYFVEMKIGTPPQKHMLIVDTGSDLLWVKCRYRRCIGANCNHGNAI 184
           IG+KM SGAD+GSSEYFV++K+GTP Q  MLI DTGSDL W+KCRYRRC G NC+ GN  
Sbjct: 136 IGMKMISGADFGSSEYFVQLKVGTPAQTFMLIADTGSDLTWLKCRYRRCFG-NCS-GNVN 195

Query: 185 HKSRNDQREKMGRVFYANRSSSFVAVPCSSNECSDEFTHFFVLRDCKVPTDPCRYDYSYA 244
           HKS+N+++++      AN+SS+F  V CSS  C++     F + +C  PT PC YDYSYA
Sbjct: 196 HKSKNEKKQRFRHALLANQSSTFKTVSCSSTMCTNNLAELFAVAECDTPTSPCVYDYSYA 255

Query: 245 GGSSARGLFAVETLTVNLTDGTEKQLNDSVIGCTESASGKVFKGADGVVGLGANVFSFTY 304
           GG+SA+G+FA ETLTV LT+G EKQL +S+IGCTE   G VF GADGV+GLG + +S TY
Sbjct: 256 GGASAKGIFAWETLTVGLTNGKEKQLRNSIIGCTEIVQGNVFDGADGVMGLGTSSYSLTY 315

Query: 305 KAGQNFQGGGFTYCLVDHLSNRLATSYFILGPPSPATRTAATTTAVTGATTKTALLASDM 364
           KA +N  GGGF+YCLVDHL+++ A SYF+LG P+P+T  + ++       + T L   D 
Sbjct: 316 KAAENANGGGFSYCLVDHLTDQRAVSYFVLGVPTPSTSASTSSAKPPAKMSYTKLYVGDP 375

Query: 365 FRGLYGMGLNGISVDGTILDIPPRVWDTNSSGGTIIDTGTSLTMLTMPAYEMVSEAFLSK 424
           +   YG+ L GIS DG +L+IPPRVWD+    GTIID+GTSLT+L  PA+++V E   S+
Sbjct: 376 YSSFYGVDLIGISADGQMLNIPPRVWDSYKGCGTIIDSGTSLTVLATPAFDVVMEVLTSR 435

Query: 425 FSNLPRLSFDPFDICFNNTMYHNSMAPKLRLHFSDGAVFQPPSKSYILPIAGDTSCLGIV 484
                ++  +PF+ CFNN+ Y + MAPKLR HF DG VF+PP+KSYI+ +    SC+GIV
Sbjct: 436 LKQFQQIEIEPFNFCFNNSQYTHDMAPKLRFHFGDGTVFEPPTKSYIVSVGEFISCIGIV 495

Query: 485 PVPFPGTNVIGNILQQNYLWQFDFNKNTVTFTPSECV 502
            +PFP  N+IGNILQQN+LWQFDF K  V F  SEC+
Sbjct: 496 SMPFPSLNIIGNILQQNHLWQFDFQKRRVGFAASECI 530

[Close]

BLAST of Sed0012612 vs. ExPASy TrEMBL
Match: A0A5D3B701 (Aspartic proteinase CDR1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold104G00400 PE=3 SV=1)

HSP 1 Score: 500.7 bits (1288), Expect = 6.9e-138
Identity = 238/460 (51.74%), Postives = 316/460 (68.70%), Query Frame = 0


Query: 53  MNAEDMNDRVKDIHMHDSSRHRMLT-----------ALRVRQLVETRVQGLSTTELPAPL 112
           M  +D+++R+KDIH HD +RHR ++            LR      T+V+   +  LP   
Sbjct: 1   MKIQDLHERMKDIHEHDRNRHRSISKSMNQKQIEDARLRAEAEAATQVEVAKSAILPPAT 60

Query: 113 ATGIGLKMFSGADYGSSEYFVEMKIGTPPQKHMLIVDTGSDLLWVKCRYRRCIGANCNHG 172
           +T IG+KM SGAD+GSSEYFV++K+GTP Q  MLI DTGSDL W+KCRYRRC G NC+ G
Sbjct: 61  STPIGMKMISGADFGSSEYFVQLKVGTPAQTFMLIADTGSDLTWLKCRYRRCFG-NCS-G 120

Query: 173 NAIHKSRNDQREKMGRVFYANRSSSFVAVPCSSNECSDEFTHFFVLRDCKVPTDPCRYDY 232
           N  HKS+N+++++      AN+SS+F  V CSS  C++     F + +C  PT PC YDY
Sbjct: 121 NVNHKSKNEKKQRFRHALLANQSSTFKTVSCSSTMCTNNLAELFAVAECDTPTSPCVYDY 180

Query: 233 SYAGGSSARGLFAVETLTVNLTDGTEKQLNDSVIGCTESASGKVFKGADGVVGLGANVFS 292
           SYAGG+SA+G+FA ETLTV LT+G EKQL +S+IGCTE   G VF GADGV+GLG + +S
Sbjct: 181 SYAGGASAKGIFAWETLTVGLTNGKEKQLRNSIIGCTEIVQGNVFDGADGVMGLGTSSYS 240

Query: 293 FTYKAGQNFQGGGFTYCLVDHLSNRLATSYFILGPPSPATRTAATTTAVTGATTKTALLA 352
            TYKA +N  GGGF+YCLVDHL+++ A SYF+LG P+P+T  + ++       + T L  
Sbjct: 241 LTYKAAENANGGGFSYCLVDHLTDQRAVSYFVLGVPTPSTSASTSSAKPPAKMSYTKLYV 300

Query: 353 SDMFRGLYGMGLNGISVDGTILDIPPRVWDTNSSGGTIIDTGTSLTMLTMPAYEMVSEAF 412
            D +   YG+ L GIS DG +L+IPPRVWD+    GTIID+GTSLT+L  PA+++V E  
Sbjct: 301 GDPYSSFYGVDLIGISADGQMLNIPPRVWDSYKGCGTIIDSGTSLTVLATPAFDVVMEVL 360

Query: 413 LSKFSNLPRLSFDPFDICFNNTMYHNSMAPKLRLHFSDGAVFQPPSKSYILPIAGDTSCL 472
            S+     ++  +PF+ CFNN+ Y + MAPKLR HF DG VF+PP+KSYI+ +    SC+
Sbjct: 361 TSRLKQFQQIEIEPFNFCFNNSQYTHDMAPKLRFHFGDGTVFEPPTKSYIVSVGEFISCI 420

Query: 473 GIVPVPFPGTNVIGNILQQNYLWQFDFNKNTVTFTPSECV 502
           GIV +PFP  N+IGNILQQN+LWQFDF K  V F  SEC+
Sbjct: 421 GIVSMPFPSLNIIGNILQQNHLWQFDFQKRRVGFAASECI 458

[Close]

BLAST of Sed0012612 vs. ExPASy TrEMBL
Match: A0A6J1L6Y2 (aspartic proteinase NANA, chloroplast-like OS=Cucurbita maxima OX=3661 GN=LOC111499734 PE=3 SV=1)

HSP 1 Score: 477.6 bits (1228), Expect = 6.2e-131
Identity = 237/490 (48.37%), Postives = 320/490 (65.31%), Query Frame = 0


Query: 23  NEVNAGGQEKVRLNMMHRHHPKIVERLKINMNAEDMNDRVKDIHMHDSSRHRMLTA---- 82
           ++ N   QE VRL+++HRHHP++V+RL   +  + M DR+KDI  HD SR R ++A    
Sbjct: 37  SDANNEEQEFVRLDLIHRHHPEVVKRLHDEIKVDKMEDRIKDIRYHDQSRLRAISAHLNW 96

Query: 83  LRVRQLVETRVQGLSTTELPAPLATGIGLKMFSGADYGSSEYFVEMKIGTPPQKHMLIVD 142
            +V +  E +V+  S +  P    T I LK + GAD+GSSE+FV++K+GTPPQK  +I D
Sbjct: 97  TKVVENAEEKVKEASGSNHPPHSQTPIALKTYPGADFGSSEFFVQLKVGTPPQKFTMIAD 156

Query: 143 TGSDLLWVKCRYRRCIGANCNHGNAIHKSRNDQREKMGRVFYANRSSSFVAVPCSSNECS 202
           TGSDLLW +CRYRRC G +C++ + IHK RN  RE+     YAN+SSSF  +PCSS +C 
Sbjct: 157 TGSDLLWTRCRYRRCRG-DCSNPSPIHKMRNKMRERFNYALYANQSSSFSPIPCSSKQCI 216

Query: 203 DEFTHFFVLRDCKVPTDPCRYDYSYAGGSSARGLFAVETLTVNLTDGTEKQLNDSVIGCT 262
            +F+      DC  P  PC Y YSY  G  A G+FA ET+TV LT+G EKQL D + GCT
Sbjct: 217 QDFSELGGQPDCPTPNTPCSYTYSYLSGDRAMGIFATETVTVRLTNGKEKQLKDILYGCT 276

Query: 263 ESASGKVF-KGADGVVGLGANVFSFTYKAGQNFQGGGFTYCLVDHLSNRLATSYFILGPP 322
           E  +   F  GADG++GLG++++SF YKA +N  GGGF+YCL DHL N  A SYF+ G P
Sbjct: 277 EEMTDSQFLDGADGLIGLGSSIYSFVYKAAENNVGGGFSYCLADHLRNITAISYFVFGTP 336

Query: 323 SPATRTAATTTAVTGATTKTALLASDMFRGLYGMGLNGISVDGTILDIPPRVWDTNSSGG 382
           SP T +A+T++ + G    T L     +   YG+ L+GISVDG IL+IPP VW+  S  G
Sbjct: 337 SPKTFSASTSSPI-GPPATTKLFTGGRYSCYYGVQLSGISVDGQILNIPPHVWNIKSGCG 396

Query: 383 TIIDTGTSLTMLTMPAYEMVSEAFLSKFSNLPRLSFD-------PFDICFNNTMYHNSMA 442
           TI+DTGTSLTMLT PA++ V EA   K     R+  D        F +CFN+T ++  M 
Sbjct: 397 TILDTGTSLTMLTAPAHDAVIEAMAPKIEKFGRMEKDVKGEREKNFKLCFNDTEWNFGML 456

Query: 443 PKLRLHFSDGAVFQPPSKSYILPIAGDTSCLGIVPVPFPGTNVIGNILQQNYLWQFDFNK 501
           PKL  HF DGAVF+PP +SYI+  +   SC+ I  +PFP  N++GNI+QQ ++W++D  K
Sbjct: 457 PKLGFHFEDGAVFEPPDRSYIVSASYQCSCIAITSLPFPSINILGNIIQQTFIWKYDLLK 516

[Close]

BLAST of Sed0012612 vs. ExPASy TrEMBL
Match: A0A6J1G810 (aspartic proteinase NANA, chloroplast-like OS=Cucurbita moschata OX=3662 GN=LOC111451585 PE=3 SV=1)

HSP 1 Score: 474.2 bits (1219), Expect = 6.9e-130
Identity = 241/492 (48.98%), Postives = 316/492 (64.23%), Query Frame = 0


Query: 24  EVNAGGQEK--VRLNMMHRHHPKIVERLKINMNAEDMNDRVKDIHMHDSSRHRMLTALR- 83
           E +A  +EK  VRL+++HRHHP++V+RL   +  + M DR+KDI  HD SR R ++    
Sbjct: 34  ESDANNEEKEFVRLDLIHRHHPEVVKRLHDEIKVDKMEDRIKDIRYHDQSRLRAISVHMN 93

Query: 84  ----VRQLVETRVQGLSTTELPAPLATGIGLKMFSGADYGSSEYFVEMKIGTPPQKHMLI 143
               V    E   +  S++ LP    T I LK + GAD+GSSE+FV++K+GTPPQK  +I
Sbjct: 94  WTKVVENAEEKEKKEASSSNLPPQSQTPIALKTYPGADFGSSEFFVQLKLGTPPQKFTMI 153

Query: 144 VDTGSDLLWVKCRYRRCIGANCNHGNAIHKSRNDQREKMGRVFYANRSSSFVAVPCSSNE 203
            DTGSDLLW +CRYRRC G +C++ + IHK RN  RE+     YAN+SSSF  +PCSS +
Sbjct: 154 ADTGSDLLWTRCRYRRCRG-DCSNPSPIHKMRNRMRERFIYALYANQSSSFSPIPCSSKQ 213

Query: 204 CSDEFTHFFVLRDCKVPTDPCRYDYSYAGGSSARGLFAVETLTVNLTDGTEKQLNDSVIG 263
           C  +F+      DC  P  PC Y YSY  G  A G+FA ET+TV LT+G EKQL D + G
Sbjct: 214 CIQDFSELGGQPDCPTPNSPCSYTYSYLSGDRAMGIFATETVTVRLTNGKEKQLKDILYG 273

Query: 264 CTESASGKVF-KGADGVVGLGANVFSFTYKAGQNFQGGGFTYCLVDHLSNRLATSYFILG 323
           CTE  +   F  GADG++GLG++++SF YKA +N  GGGF+YCL DHL N  A SYF+ G
Sbjct: 274 CTEEITDSQFLDGADGLIGLGSSIYSFVYKAAENNVGGGFSYCLADHLRNITAISYFVFG 333

Query: 324 PPSPATRTAATTTAVTGATTKTALLASDMFRGLYGMGLNGISVDGTILDIPPRVWDTNSS 383
            PSP T  AA+T++  G    T L+    +   YG+ L GISVDG IL+IPP VW+  S 
Sbjct: 334 TPSPKT-FAASTSSPIGPPATTRLITGGRYSCYYGVQLAGISVDGQILNIPPHVWNIKSG 393

Query: 384 GGTIIDTGTSLTMLTMPAYEMVSEAFLSKFSNLPRLSFD-------PFDICFNNTMYHNS 443
            GTI+DTGTSLTMLT PA++ V EA   K     R+  D        F +CFN+T ++  
Sbjct: 394 CGTILDTGTSLTMLTAPAHDAVIEAMAPKIEKFGRMERDVKGEREKNFKLCFNDTQWNFG 453

Query: 444 MAPKLRLHFSDGAVFQPPSKSYILPIAGDTSCLGIVPVPFPGTNVIGNILQQNYLWQFDF 501
           M PKL  HF  GAVF+PP +SYI+  +   SC+ I  +PFP  N++GNI+QQ YLWQFD 
Sbjct: 454 MLPKLGFHFEGGAVFEPPDRSYIVSASYQCSCIAITSLPFPSINILGNIIQQTYLWQFDL 513

[Close]

BLAST of Sed0012612 vs. TAIR 10
Match: AT3G12700.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 303.9 bits (777), Expect = 2.4e-82
Identity = 176/443 (39.73%), Postives = 242/443 (54.63%), Query Frame = 0


Query: 61  RVKDIHMHDSSRHRMLTALRVRQLVETRVQGLSTTELPAPLATGIGLKMFSGADYGSSEY 120
           R++D+   D  RH +++  R                       G+ + + SG DYG+++Y
Sbjct: 66  RIEDVIGADQKRHSLISRKR-------------------NSTVGVKMDLGSGIDYGTAQY 125

Query: 121 FVEMKIGTPPQKHMLIVDTGSDLLWVKCRYRRCIGANCNHGNAIHKSRNDQREKMGRVFY 180
           F E+++GTP +K  ++VDTGS+L WV CRYR                  D R    RVF 
Sbjct: 126 FTEIRVGTPAKKFRVVVDTGSELTWVNCRYR--------------ARGKDNR----RVFR 185

Query: 181 ANRSSSFVAVPCSSNECSDEFTHFFVLRDCKVPTDPCRYDYSYAGGSSARGLFAVETLTV 240
           A+ S SF  V C +  C  +  + F L  C  P+ PC YDY YA GS+A+G+FA ET+TV
Sbjct: 186 ADESKSFKTVGCLTQTCKVDLMNLFSLTTCPTPSTPCSYDYRYADGSAAQGVFAKETITV 245

Query: 241 NLTDGTEKQLNDSVIGCTESASGKVFKGADGVVGLGANVFSFTYKAGQNFQGGGFTYCLV 300
            LT+G   +L   +IGC+ S +G+ F+GADGV+GL  + FSFT  A  +  G  F+YCLV
Sbjct: 246 GLTNGRMARLPGHLIGCSSSFTGQSFQGADGVLGLAFSDFSFTSTA-TSLYGAKFSYCLV 305

Query: 301 DHLSNRLATSYFILGPPSPATRTAATTTAVTGATTKTALLASDMFRGLYGMGLNGISVDG 360
           DHLSN+  ++Y I G  S +T+TA   T     T              Y + + GIS+  
Sbjct: 306 DHLSNKNVSNYLIFG-SSRSTKTAFRRTTPLDLT---------RIPPFYAINVIGISLGY 365

Query: 361 TILDIPPRVWDTNSSGGTIIDTGTSLTMLTMPAYEMVSEAFLSKFSNLPRLSFD--PFDI 420
            +LDIP +VWD  S GGTI+D+GTSLT+L   AY+ V          L R+  +  P + 
Sbjct: 366 DMLDIPSQVWDATSGGGTILDSGTSLTLLADAAYKQVVTGLARYLVELKRVKPEGVPIEY 425

Query: 421 CFNNTMYHN-SMAPKLRLHFSDGAVFQPPSKSYILPIAGDTSCLGIVPVPFPGTNVIGNI 480
           CF+ T   N S  P+L  H   GA F+P  KSY++  A    CLG V    P TNVIGNI
Sbjct: 426 CFSFTSGFNVSKLPQLTFHLKGGARFEPHRKSYLVDAAPGVKCLGFVSAGTPATNVIGNI 460

Query: 481 LQQNYLWQFDFNKNTVTFTPSEC 501
           +QQNYLW+FD   +T++F PS C
Sbjct: 486 MQQNYLWEFDLMASTLSFAPSAC 460

[Close]

BLAST of Sed0012612 vs. TAIR 10
Match: AT3G25700.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 213.4 bits (542), Expect = 4.2e-55
Identity = 145/431 (33.64%), Postives = 206/431 (47.80%), Query Frame = 0


Query: 88  RVQGLSTTELPAPLATGIGLKMFSGADYGSSEYFVEMKIGTPPQKHMLIVDTGSDLLWVK 147
           R+  LS    P P    +   + SGA  GS +YFV+++IG PPQ  +LI DTGSDL+WVK
Sbjct: 55  RLHFLSLRRKPIPF---VKSPVVSGAASGSGQYFVDLRIGQPPQSLLLIADTGSDLVWVK 114

Query: 148 CRYRRCIGANCNHGNAIHKSRNDQREKMGRVFYANRSSSFVAVPCSSNECSDEFTHFFVL 207
           C   R    NC+H                 VF+   SS+F    C    C        V 
Sbjct: 115 CSACR----NCSH------------HSPATVFFPRHSSTFSPAHCYDPVCR------LVP 174

Query: 208 RDCKVPT-------DPCRYDYSYAGGSSARGLFAVETLTVNLTDGTEKQLNDSVIGC--- 267
           +  + P          C Y+Y YA GS   GLFA ET ++  + G E +L     GC   
Sbjct: 175 KPDRAPICNHTRIHSTCHYEYGYADGSLTSGLFARETTSLKTSSGKEARLKSVAFGCGFR 234

Query: 268 --TESASGKVFKGADGVVGLGANVFSFTYKAGQNFQGGGFTYCLVDHLSNRLATSYFILG 327
              +S SG  F GA+GV+GLG    SF  + G+ F G  F+YCL+D+  +   TSY I+G
Sbjct: 235 ISGQSVSGTSFNGANGVMGLGRGPISFASQLGRRF-GNKFSYCLMDYTLSPPPTSYLIIG 294

Query: 328 PPSPATRTAATTTAVTGATTKTALLASDMFRGLYGMGLNGISVDGTILDIPPRVW--DTN 387
                      T  +T   + T           Y + L  + V+G  L I P +W  D +
Sbjct: 295 NGGDGISKLFFTPLLTNPLSPT----------FYYVKLKSVFVNGAKLRIDPSIWEIDDS 354

Query: 388 SSGGTIIDTGTSLTMLTMPAYEMVSEAFLSKFSNLPRLSFDP-FDICFN--NTMYHNSMA 447
            +GGT++D+GT+L  L  PAY  V  A   +       +  P FD+C N         + 
Sbjct: 355 GNGGTVVDSGTTLAFLAEPAYRSVIAAVRRRVKLPIADALTPGFDLCVNVSGVTKPEKIL 414

Query: 448 PKLRLHFSDGAVFQPPSKSYILPIAGDTSCLGIVPV-PFPGTNVIGNILQQNYLWQFDFN 501
           P+L+  FS GAVF PP ++Y +       CL I  V P  G +VIGN++QQ +L++FD +
Sbjct: 415 PRLKFEFSGGAVFVPPPRNYFIETEEQIQCLAIQSVDPKVGFSVIGNLMQQGFLFEFDRD 449

[Close]

BLAST of Sed0012612 vs. TAIR 10
Match: AT2G42980.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 184.1 bits (466), Expect = 2.7e-46
Identity = 146/484 (30.17%), Postives = 228/484 (47.11%), Query Frame = 0


Query: 30  QEKVRLNMMHRHHPKIVERLKINMNAEDMNDRVKDIHMHDSSRHRMLTALRVRQLVETRV 89
           +E V+     +   K      +++  +D+  R+K +H   +   +     +VR+ + + +
Sbjct: 75  RESVKPQSRIKQETKRTTHSVVDLQIQDLT-RIKTLHARFNKSKKQKNE-KVRKKITSDI 134

Query: 90  QGLSTTEL-PAPLATGIGLKMFSGADYGSSEYFVEMKIGTPPQKHMLIVDTGSDLLWVKC 149
             +   E+ P  L       + SG   GS EYF+++ +GTPP+   LI+DTGSDL W++C
Sbjct: 135 SLVGAPEVSPGKLIA----TLESGMTLGSGEYFMDVLVGTPPKHFSLILDTGSDLNWLQC 194

Query: 150 RYRRCIGANCNHGNAIHKSRNDQREKMGRVFYANRSSSFVAVPCSSNECSDEFTHFFVLR 209
               C   +C H N             G  +    S+SF  + C+   CS   +    ++
Sbjct: 195 --LPCY--DCFHQN-------------GMFYDPKTSASFKNITCNDPRCSLISSPDPPVQ 254

Query: 210 DCKVPTDPCRYDYSYAGGSSARGLFAVETLTVNLT----DGTEKQLNDSVIGCTESASGK 269
            C+     C Y Y Y   S+  G FAVET TVNLT      +E ++ + + GC     G 
Sbjct: 255 -CESDNQSCPYFYWYGDRSNTTGDFAVETFTVNLTTTEGGSSEYKVGNMMFGCGHWNRG- 314

Query: 270 VFKGADGVVGLGANVFSFTYKAGQNFQGGGFTYCLVDHLSNRLATSYFILGPPSPATR-- 329
           +F GA G++GLG    SF+ +  Q+  G  F+YCLVD  SN   +S  I G         
Sbjct: 315 LFSGASGLLGLGRGPLSFSSQL-QSLYGHSFSYCLVDRNSNTNVSSKLIFGEDKDLLNHT 374

Query: 330 TAATTTAVTGATTKTALLASDMFRGLYGMGLNGISVDGTILDIPPRVWDTNS--SGGTII 389
               T+ V G          +     Y + +  I V G  LDIP   W+ +S   GGTII
Sbjct: 375 NLNFTSFVNG--------KENSVETFYYIQIKSILVGGKALDIPEETWNISSDGDGGTII 434

Query: 390 DTGTSLTMLTMPAYEMVSEAFLSKF-SNLPRL-SFDPFDICFN--NTMYHNSMAPKLRLH 449
           D+GT+L+    PAYE++   F  K   N P    F   D CFN      +N   P+L + 
Sbjct: 435 DSGTTLSYFAEPAYEIIKNKFAEKMKENYPIFRDFPVLDPCFNVSGIEENNIHLPELGIA 494

Query: 450 FSDGAVFQPPSKSYILPIAGDTSCLGIVPVPFPGTNVIGNILQQNYLWQFDFNKNTVTFT 501
           F DG V+  P+++  + ++ D  CL I+  P    ++IGN  QQN+   +D  ++ + FT
Sbjct: 495 FVDGTVWNFPAENSFIWLSEDLVCLAILGTPKSTFSIIGNYQQQNFHILYDTKRSRLGFT 524

[Close]

BLAST of Sed0012612 vs. TAIR 10
Match: AT3G59080.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 178.3 bits (451), Expect = 1.5e-44
Identity = 125/400 (31.25%), Postives = 196/400 (49.00%), Query Frame = 0


Query: 111 SGADYGSSEYFVEMKIGTPPQKHMLIVDTGSDLLWVKCRYRRCIGANCNHGNAIHKSRND 170
           SG   GS EYF+++ +G+PP+   LI+DTGSDL W++C         C           D
Sbjct: 161 SGMTLGSGEYFMDVLVGSPPKHFSLILDTGSDLNWIQC-------LPC----------YD 220

Query: 171 QREKMGRVFYANRSSSFVAVPCSSNECSDEFTHFFVLRDCKVPTDPCRYDYSYAGGSSAR 230
             ++ G  +    S+S+  + C+   C+   +    +  CK     C Y Y Y   S+  
Sbjct: 221 CFQQNGAFYDPKASASYKNITCNDQRCNLVSSPDPPM-PCKSDNQSCPYYYWYGDSSNTT 280

Query: 231 GLFAVETLTVNL-TDGTEKQL---NDSVIGCTESASGKVFKGADGVVGLGANVFSFTYKA 290
           G FAVET TVNL T+G   +L    + + GC     G +F GA G++GLG    SF+ + 
Sbjct: 281 GDFAVETFTVNLTTNGGSSELYNVENMMFGCGHWNRG-LFHGAAGLLGLGRGPLSFSSQL 340

Query: 291 GQNFQGGGFTYCLVDHLSNRLATSYFILGPPSP--ATRTAATTTAVTGATTKTALLASDM 350
            Q+  G  F+YCLVD  S+   +S  I G      +      T+ V G          ++
Sbjct: 341 -QSLYGHSFSYCLVDRNSDTNVSSKLIFGEDKDLLSHPNLNFTSFVAG--------KENL 400

Query: 351 FRGLYGMGLNGISVDGTILDIPPRVWDTNS--SGGTIIDTGTSLTMLTMPAYEMVSEAFL 410
               Y + +  I V G +L+IP   W+ +S  +GGTIID+GT+L+    PAYE +     
Sbjct: 401 VDTFYYVQIKSILVAGEVLNIPEETWNISSDGAGGTIIDSGTTLSYFAEPAYEFIKNKIA 460

Query: 411 SKFSNLPRL--SFDPFDICFNNTMYHNSMAPKLRLHFSDGAVFQPPSKSYILPIAGDTSC 470
            K      +   F   D CFN +  HN   P+L + F+DGAV+  P+++  + +  D  C
Sbjct: 461 EKAKGKYPVYRDFPILDPCFNVSGIHNVQLPELGIAFADGAVWNFPTENSFIWLNEDLVC 520

Query: 471 LGIVPVPFPGTNVIGNILQQNYLWQFDFNKNTVTFTPSEC 501
           L ++  P    ++IGN  QQN+   +D  ++ + + P++C
Sbjct: 521 LAMLGTPKSAFSIIGNYQQQNFHILYDTKRSRLGYAPTKC 532

[Close]

BLAST of Sed0012612 vs. TAIR 10
Match: AT3G18490.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 169.5 bits (428), Expect = 7.0e-42
Identity = 116/395 (29.37%), Postives = 187/395 (47.34%), Query Frame = 0


Query: 111 SGADYGSSEYFVEMKIGTPPQKHMLIVDTGSDLLWVKCRYRRCIGANCNHGNAIHKSRND 170
           SGA  GS EYF  + +GTP ++  L++DTGSD+ W++C    C  A+C            
Sbjct: 153 SGASQGSGEYFSRIGVGTPAKEMYLVLDTGSDVNWIQC--EPC--ADC------------ 212

Query: 171 QREKMGRVFYANRSSSFVAVPCSSNECSDEFTHFFVLRDCKVPTDPCRYDYSYAGGSSAR 230
             ++   VF    SS++ ++ CS+ +CS       +L      ++ C Y  SY  GS   
Sbjct: 213 -YQQSDPVFNPTSSSTYKSLTCSAPQCS-------LLETSACRSNKCLYQVSYGDGSFTV 272

Query: 231 GLFAVETLTVNLTDGTEKQLNDSVIGCTESASGKVFKGADGVVGLGANVFSFTYKAGQNF 290
           G  A +T+T     G   ++N+  +GC     G +F GA G++GLG  V S T       
Sbjct: 273 GELATDTVTF----GNSGKINNVALGCGHDNEG-LFTGAAGLLGLGGGVLSIT----NQM 332

Query: 291 QGGGFTYCLVDHLSNRLATSYFILGPPSPATRTAATTTAVTGATTKTALLASDMFRGLYG 350
           +   F+YCLVD  S + ++  F              +  + G      LL +      Y 
Sbjct: 333 KATSFSYCLVDRDSGKSSSLDF-------------NSVQLGGGDATAPLLRNKKIDTFYY 392

Query: 351 MGLNGISVDGTILDIPPRVWDTNS--SGGTIIDTGTSLTMLTMPAYEMVSEAFLSKFSNL 410
           +GL+G SV G  + +P  ++D ++  SGG I+D GT++T L   AY  + +AFL    NL
Sbjct: 393 VGLSGFSVGGEKVVLPDAIFDVDASGSGGVILDCGTAVTRLQTQAYNSLRDAFLKLTVNL 452

Query: 411 PR--LSFDPFDICFNNTMYHNSMAPKLRLHFSDGAVFQPPSKSYILPI-AGDTSCLGIVP 470
            +   S   FD C++ +       P +  HF+ G     P+K+Y++P+    T C    P
Sbjct: 453 KKGSSSISLFDTCYDFSSLSTVKVPTVAFHFTGGKSLDLPAKNYLIPVDDSGTFCFAFAP 500

Query: 471 VPFPGTNVIGNILQQNYLWQFDFNKNTVTFTPSEC 501
                 ++IGN+ QQ     +D +KN +  + ++C
Sbjct: 513 TS-SSLSIIGNVQQQGTRITYDLSKNVIGLSGNKC 500

The following BLAST results are available for this feature:

BLAST of Sed0012612 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038901983.1	2.4e-153	53.57	aspartic proteinase NANA, chloroplast [Benincasa hispida]	[more]
XP_004140022.2	1.2e-147	52.59	aspartic proteinase NANA, chloroplast [Cucumis sativus] >KGN46781.1 hypothetical...	[more]
XP_008456273.1	2.0e-144	49.52	PREDICTED: aspartic proteinase CDR1 [Cucumis melo]	[more]
KAA0033565.1	1.4e-137	51.74	aspartic proteinase CDR1 [Cucumis melo var. makuwa] >TYJ95622.1 aspartic protein...	[more]
XP_023533886.1	9.9e-131	48.47	aspartic proteinase NANA, chloroplast-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Sed0012612 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LTW4	3.3e-81	39.73	Aspartic proteinase NANA, chloroplast OS=Arabidopsis thaliana OX=3702 GN=NANA PE...	[more]
Q9LS40	9.8e-41	29.37	Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASP...	[more]
Q9LHE3	1.9e-39	28.93	Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASP...	[more]
Q9LNJ3	4.1e-39	28.54	Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ...	[more]
Q766C3	1.1e-34	30.10	Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S...	[more]

BLAST of Sed0012612 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KG92	5.6e-148	52.59	Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G13439...	[more]
A0A1S3C2F3	9.9e-145	49.52	aspartic proteinase CDR1 OS=Cucumis melo OX=3656 GN=LOC103496268 PE=3 SV=1	[more]
A0A5D3B701	6.9e-138	51.74	Aspartic proteinase CDR1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol...	[more]
A0A6J1L6Y2	6.2e-131	48.37	aspartic proteinase NANA, chloroplast-like OS=Cucurbita maxima OX=3661 GN=LOC111...	[more]
A0A6J1G810	6.9e-130	48.98	aspartic proteinase NANA, chloroplast-like OS=Cucurbita moschata OX=3662 GN=LOC1...	[more]

BLAST of Sed0012612 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G12700.1	2.4e-82	39.73	Eukaryotic aspartyl protease family protein	[more]
AT3G25700.1	4.2e-55	33.64	Eukaryotic aspartyl protease family protein	[more]
AT2G42980.1	2.7e-46	30.17	Eukaryotic aspartyl protease family protein	[more]
AT3G59080.1	1.5e-44	31.25	Eukaryotic aspartyl protease family protein	[more]
AT3G18490.1	7.0e-42	29.37	Eukaryotic aspartyl protease family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001461	Aspartic peptidase A1 family	PRINTS	PR00792	PEPSIN	coord: 472..487 score: 21.86 coord: 126..146 score: 56.02 coord: 378..389 score: 50.05
IPR032861	Xylanase inhibitor, N-terminal	PFAM	PF14543	TAXi_N	coord: 120..315 e-value: 1.4E-44 score: 152.4
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 107..315 e-value: 1.3E-36 score: 128.5
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 319..501 e-value: 2.3E-39 score: 136.9
IPR021109	Aspartic peptidase domain superfamily	SUPERFAMILY	50630	Acid proteases	coord: 112..500
IPR032799	Xylanase inhibitor, C-terminal	PFAM	PF14541	TAXi_C	coord: 349..496 e-value: 1.6E-31 score: 109.3
None	No IPR available	PANTHER	PTHR47967:SF69	ASPARTIC PROTEINASE NANA, CHLOROPLAST	coord: 14..500
None	No IPR available	PANTHER	PTHR47967	OS07G0603500 PROTEIN-RELATED	coord: 14..500
IPR001969	Aspartic peptidase, active site	PROSITE	PS00141	ASP_PROTEASE	coord: 135..146
IPR033121	Peptidase family A1 domain	PROSITE	PS51767	PEPTIDASE_A1	coord: 120..496 score: 35.351368
IPR034161	Pepsin-like domain, plant	CDD	cd05476	pepsin_A_like_plant	coord: 119..500 e-value: 2.71294E-65 score: 210.197

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Sed0012612.1	Sed0012612.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0004190	aspartic-type endopeptidase activity